pycom12g00240

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016922
Physical Location & Seq
Forward (+)
317991 .. 318548
558 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g00240.1

Sequence Viewer

Length: 558 bp
ATGGATAATACTTTTATACGCAATTTATTGGGGGGTGCCGCTGCTAAGATTGCTTCCCAGATGCCATTGGCTGCCGGGTTTCTTACTTTTGTGTGCATTGAGTGCTTTGTGGATCAACTGGTTGCTCCGATCAAACCCTTAACCGGCCAGTACTTGGCCAAGTATTTACTTGCCTGCAGTGGCCCCGTGGTCTCCAGATACTTGGACATTTACGTTGCTTACATCCTAAGCTACAAATCCAACGTCGACAACCTGCTGAAACAGCTCAAGAAGCTTAAGGCCACACGAGATATGGTGATCCAAACCGTCGAAGATGCAAAAAGGAAGGGAGAAGTGGCGGTCACAGCTGAAGTCGAGAAGTGGTTGAGCAGTGTCGATAGGCTGACCGAAGAAGCCGCCCAGTTTTTCGCATACAAAGGGGACGCAAACAGCGGGTGGTTCCGTTGGTTGTACGGTCTGTTGATGTCGAGATTCCAATTGAGCAAGAAAGCAAAGAAGATGATAGAGCTTCTCGTTCAGCTCCAAGCGGATGGAATTTTTGCTACTGTTTCTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

186

Amino Acids

20.66

Weight (kDa)

9.18

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016922)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18500
prunus_persica Prupe.7G003000_v2.0.a1
pyrus_communis pycom12g00240
rosa_chinensis RchiOBHm_Chr3g0497431
rosa_laevigata RLG00000022462 RLG00000022464
rosa_roxburghii Rroxscaffold_166G00440670 Rroxscaffold_6G00388050
rosa_rugosa Rorug03G0293000 Rorug03G0293000
rosa_samantha Rh3AG336500 Rh3BG374800 Rh3CG370700 Rh3DG371600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 35
AccB7I CCANNNNNTGG 1 cut(s) 154
AccI GTMKAC 1 cut(s) 246
AciI CCGC 5 cut(s) 39, 338, 396, 432, 527
AclWI GGATC 2 cut(s) 120, 292
AcoI YGGCCR 2 cut(s) 145, 156
AcsI RAATTY 1 cut(s) 534
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 2 cut(s) 152, 452
AfiI CCNNNNNNNGG 2 cut(s) 143, 154
AflII CTTAAG 1 cut(s) 275
AluBI AGCT 6 cut(s) 231, 265, 274, 347, 508, 520
AluI AGCT 6 cut(s) 231, 265, 274, 347, 508, 520
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 2 cut(s) 120, 292
AoxI GGCC 4 cut(s) 145, 156, 181, 279
ApeKI GCWGC 2 cut(s) 41, 71
ApoI RAATTY 1 cut(s) 534
ArsI GACNNNNNNTTYG 2 cut(s) 228, 260
AspS9I GGNCC 1 cut(s) 182
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 307
BalI TGGCCA 1 cut(s) 158
BanI GGYRCC 1 cut(s) 35
BauI CACGAG 1 cut(s) 285
BbvI GCAGC 2 cut(s) 28, 58
BccI CCATC 1 cut(s) 524
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 196
BfmI CTRYAG 1 cut(s) 175
BfrI CTTAAG 1 cut(s) 275
BfuAI ACCTGC 1 cut(s) 261
BisI GCNGC 4 cut(s) 39, 42, 72, 396
BlsI GCNGC 4 cut(s) 40, 43, 73, 397
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 76
BmgT120I GGNCC 1 cut(s) 182
BmiI GGNNCC 3 cut(s) 37, 184, 440
BmrFI CCNGG 1 cut(s) 76
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 2 cut(s) 51, 304
BmuI ACTGGG 1 cut(s) 394
BpmI CTGGAG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 227
BpuEI CTTGAG 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 76
BsaI GGTCTC 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 2 cut(s) 143, 154
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 3 cut(s) 123, 148, 400
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 222, 535
BseLI CCNNNNNNNGG 2 cut(s) 143, 154
BseNI ACTGG 3 cut(s) 123, 148, 400
BseXI GCAGC 2 cut(s) 28, 58
BshFI GGCC 4 cut(s) 147, 158, 183, 281
BshNI GGYRCC 1 cut(s) 35
BsiSI CCGG 2 cut(s) 75, 144
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 2 cut(s) 143, 154
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 4 cut(s) 147, 158, 183, 281
Bso31I GGTCTC 1 cut(s) 196
Bsp143I GATC 3 cut(s) 112, 129, 297
BspACI CCGC 5 cut(s) 39, 338, 396, 432, 527
BspANI GGCC 4 cut(s) 147, 158, 183, 281
BspLI GGNNCC 3 cut(s) 37, 184, 440
BspMAI CTGCAG 1 cut(s) 179
BspMI ACCTGC 1 cut(s) 261
BspPI GGATC 2 cut(s) 120, 292
BspT107I GGYRCC 1 cut(s) 35
BspTI CTTAAG 1 cut(s) 275
BspTNI GGTCTC 1 cut(s) 196
BsrFI RCCGGY 1 cut(s) 143
BsrI ACTGG 3 cut(s) 123, 148, 400
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 3 cut(s) 112, 129, 297
BssSI CACGAG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 285
Bst4CI ACNGT 3 cut(s) 307, 455, 547
BstAFI CTTAAG 1 cut(s) 275
BstAPI GCANNNNNTGC 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 175
BstDEI CTNAG 2 cut(s) 45, 227
BstDSI CCRYGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 222, 535
BstKTI GATC 3 cut(s) 115, 132, 300
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 3 cut(s) 112, 129, 297
BstMWI GCNNNNNNNGC 5 cut(s) 50, 102, 262, 271, 344
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 28, 58
BstXI CCANNNNNNTGG 2 cut(s) 202, 530
BsuRI GGCC 4 cut(s) 147, 158, 183, 281
BtgI CCRYGG 1 cut(s) 186
BtsCI GGATG 2 cut(s) 222, 535
BtsI GCAGTG 2 cut(s) 184, 376
BtsIMutI CAGTG 2 cut(s) 184, 376
BveI ACCTGC 1 cut(s) 261
Cac8I GCNNGC 1 cut(s) 175
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 1 cut(s) 182
CseI GACGC 1 cut(s) 431
Csp6I GTAC 2 cut(s) 151, 451
CviQI GTAC 2 cut(s) 151, 451
DdeI CTNAG 2 cut(s) 45, 227
DpnI GATC 3 cut(s) 114, 131, 299
DpnII GATC 3 cut(s) 112, 129, 297
EaeI YGGCCR 2 cut(s) 145, 156
Eco31I GGTCTC 1 cut(s) 196
Eco57I CTGAAG 1 cut(s) 369
FaiI YATR 3 cut(s) 17, 293, 412
FaqI GGGAC 1 cut(s) 434
FauI CCCGC 1 cut(s) 425
FblI GTMKAC 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 39, 42, 72, 396
FokI GGATG 2 cut(s) 209, 542
Fsp4HI GCNGC 4 cut(s) 39, 42, 72, 396
GluI GCNGC 4 cut(s) 39, 42, 72, 396
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 4 cut(s) 147, 158, 183, 281
HapII CCGG 2 cut(s) 75, 144
HgaI GACGC 1 cut(s) 431
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 1 cut(s) 471
HpaII CCGG 2 cut(s) 75, 144
HphI GGTGA 1 cut(s) 307
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 1 cut(s) 129
Hpy188III TCNNGA 4 cut(s) 195, 268, 355, 468
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 2 cut(s) 248, 311
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 3 cut(s) 307, 455, 547
HpyCH4IV ACGT 2 cut(s) 213, 243
HpyCH4V TGCA 3 cut(s) 96, 177, 317
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 102, 262, 271, 344
HpyF3I CTNAG 2 cut(s) 45, 227
HpySE526I ACGT 2 cut(s) 213, 243
Kzo9I GATC 3 cut(s) 112, 129, 297
LmnI GCTCC 2 cut(s) 130, 525
LpnPI CCDG 9 cut(s) 71, 88, 104, 157, 161, 187, 208, 266, 413
Lsp1109I GCAGC 2 cut(s) 28, 58
LweI GCATC 2 cut(s) 51, 304
MaeII ACGT 2 cut(s) 213, 243
MaeIII GTNAC 1 cut(s) 340
MalI GATC 3 cut(s) 114, 131, 299
MboI GATC 3 cut(s) 112, 129, 297
MboII GAAGA 3 cut(s) 323, 401, 508
MfeI CAATTG 1 cut(s) 476
MlsI TGGCCA 1 cut(s) 158
MluCI AATT 4 cut(s) 22, 476, 534, 553
MluNI TGGCCA 1 cut(s) 158
MmeI TCCRAC 1 cut(s) 264
Mox20I TGGCCA 1 cut(s) 158
MscI TGGCCA 1 cut(s) 158
MseI TTAA 3 cut(s) 140, 276, 556
Msp20I TGGCCA 1 cut(s) 158
MspA1I CMGCKG 3 cut(s) 41, 347, 432
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 2 cut(s) 75, 144
MspR9I CCNGG 1 cut(s) 76
MunI CAATTG 1 cut(s) 476
MwoI GCNNNNNNNGC 5 cut(s) 50, 102, 262, 271, 344
NciI CCSGG 1 cut(s) 76
NdeII GATC 3 cut(s) 112, 129, 297
NlaIV GGNNCC 3 cut(s) 37, 184, 440
NmuCI GTSAC 1 cut(s) 340
PfeI GAWTC 1 cut(s) 471
PflMI CCANNNNNTGG 1 cut(s) 154
PkrI GCNGC 4 cut(s) 40, 43, 73, 397
PspN4I GGNNCC 3 cut(s) 37, 184, 440
PspPI GGNCC 1 cut(s) 182
PstI CTGCAG 1 cut(s) 179
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 152, 452
RsaNI GTAC 2 cut(s) 151, 451
SalI GTCGAC 1 cut(s) 245
SaqAI TTAA 3 cut(s) 140, 276, 556
SatI GCNGC 4 cut(s) 39, 42, 72, 396
Sau3AI GATC 3 cut(s) 112, 129, 297
Sau96I GGNCC 1 cut(s) 182
ScaI AGTACT 1 cut(s) 152
ScrFI CCNGG 1 cut(s) 76
SetI ASST 9 cut(s) 216, 233, 246, 255, 267, 276, 349, 510, 522
SfaNI GCATC 2 cut(s) 51, 304
SfcI CTRYAG 1 cut(s) 175
SmlI CTYRAG 2 cut(s) 266, 275
SmoI CTYRAG 2 cut(s) 266, 275
Sse9I AATT 4 cut(s) 22, 476, 534, 553
SsiI CCGC 5 cut(s) 39, 338, 396, 432, 527
StyD4I CCNGG 1 cut(s) 74
TaaI ACNGT 3 cut(s) 307, 455, 547
TaiI ACGT 2 cut(s) 216, 246
TaqI TCGA 5 cut(s) 246, 309, 354, 375, 467
TaqII GACCGA 1 cut(s) 401
TasI AATT 4 cut(s) 22, 476, 534, 553
TatI WGTACW 1 cut(s) 150
TauI GCSGC 2 cut(s) 41, 398
TfiI GAWTC 1 cut(s) 471
Tru1I TTAA 3 cut(s) 140, 276, 556
Tru9I TTAA 3 cut(s) 140, 276, 556
TscAI CASTG 2 cut(s) 184, 376
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 41, 71
Tsp45I GTSAC 1 cut(s) 340
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 2 cut(s) 184, 376
Van91I CCANNNNNTGG 1 cut(s) 154
Vha464I CTTAAG 1 cut(s) 275
XapI RAATTY 1 cut(s) 534
XcmI CCANNNNNNNNNTGG 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 246
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.