Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

29973396 .. 29979883

Gene Structure 6488 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0293000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2328 bp

ATGGCGGGGTTCGATGGCTCCGAATCCCAGCAACAAAACCAGCTATTCAGCGTAGGGTCAGAAGTGGAAGTGAGGAGCGACGAGGACGGGTTCAAAGGAGCGTGGTTCAGAGCCACCATTGTTCAGAGCCCAGCTGCTAGCTCAGCCTCAAAGAAGAAGAAGAGGGCTCTGGTGGAGTACAAGACCTTGGTCACCGAAGACGGGTCTCAGCAGCCACTGAAAGAACAAGTCGATTCCGCTTACATAAGGCCTCTGCCGCCTCACGTGGCTGACCAAGATCTTGAATACGGCCACATAGTCGACGCCGACTACAAAGACGGTTGGTGGACCGGAGTTATTGACAAGGTTGTCAAGAAGAACTCCAAATATAGGATCTTCTTTGAGAACCCGCCCGACTTTCTGGAATTCGAGAGAGACCGTCTGCGACTCCATCAGGATTGGGTTGCCGGCTTTTGGGTTCGGCCCGACAAGCAGATACTGAGTGCTTCAGATTCTCAACAAGCGGAAGAGCATCGGCTGCGACTGCTGCCTGTCCAAAATGATTCCAACCATCCTGAGGTGGCTGCACAACCGAAAACTTCAGGTGCTGCAGGGGATAACCCTGAATCAACTAGTTCAAAGAAGAATCTTGTGGAGCAGTCAAGTTGTACCCAAAGCATCAAGGATGTAGCGCCTAATGGTGGTGCTACAGACTCGCGTCCTTTGAAAAAGTTAAAGGACGACAAAGTGTCAGAGGCTACATTATCTGTTACACCACGGCAATTGAGGAAAACTCCAAATGATAAAGAAATGCTATCTGAATTAGCCACTGCGACCAGGGAAAGCAGTGGAACTAGAGGATCAAGACGAAAGACAAAGCAAGAGAAAGATGGTGAGCTAGAAAACCACTGGATGCACTCTGCCAAAAGTAAAGGAAGGCGTTCGAAGGCTCAAGTTGGAAGTGCCCAGGAAGCGTTTATATGTGCGCTTCCTAGCCAAGAGAGACATAAGGAAAAAGGTAAGGAAGGGTATGGAGTTATTGGTTCTGCTGGAAATGTTGTTCAAAAGGAGGGCATAAGTAAAGAAGCTGAATTGTCTCTCACTATAGAGTCAAAGGTTAATGGAAAGCAGGATTCATCAGCTGAATATCCATGTCAGATTCCCAACGAAGATTTGCTGAAGCTCGTGAGAGATCAGAAGAAGAATGCAAATGGTCCAGCACAAGAAAACAGCACGGTTACATCAGCTGAACATGGCAGTGATCCAGTTTTGGATAACCTTCTCCGTAGCCTGGTTCTTTTTCCAGACATGGATTTTAAGCAGCAACCAACTGAAGGAAGCAGCCACAAAAGAAAGAGGGGCAGACCTAGAAAGGTTCCTAAAAACTTTATCGTAATCACACAAGCCTCACAGGGAGTCAGGGAACTAAATGGATCAAGAAATGTTGTTGACAAAAGTGCTGTAGAAGATCAGACACTTGATGAAGTTGCTTTGCATTTTCCCGGAGGGAGAGATGCTTCAGATTCACAAGATGCGTCCAGGAGAAATACAGCTGATGTTCCTGGAACAAGGGGCATACCAAATGAAGCTGCTAGGACCCGTGTTCAAGCATCCGGAAATATAGATGATGATGACCAACCTCTCTCCACATGGTTTGGAGGAATGCAGGGTCCAGCAAACATGGATGAATTAAGTAAAACTGTTGAACAAGTTAAGCTTGCCAGAGATTCTACTCCAGTTGATTCAGTCAGTGACACTGTGCCAGATGAGAACCAAATTATGCCATTTGTGAAGAGCTCCCCAATCTGGAAAGCAATTGAATCATTTGACGTATTCAGAATAATGCCACAAAATCCACATTTTCGTCCCTTGGTTAAATGTAAAGAGGAATATCGCGAGGGATTTGCTATTGGCAATATGGTGACCTTTTCCAGTTTGGTGGAGAAGATATCGAGTCTGCATTTTGATGACCCTATACAGGTTTTTGATAGCATTTTGGAGAGTCTTTTTGACTTGGAAAAATATGGATTCAATGTCACCGTTTTACGTGGGCGTGTGAACGAACTTTTGTCTATCAAACAAAGGCAAGGACAGTTTGAGGGCGAATCAAAAGACGCGGAAAGTAAGATAGTGGAGCAAACTCAAGAGAAATCAAAACTTGAAGATGAGGCTGATTGCATCGAGAAAAAAATAATTGAGTTAAAGGAGAAGCATGCATCGATCCAGGCAGAGATAGGGGCTAAAGAGCGTGACATTGCTAGCTTGCAGTTGAGTCTGGATGCTATCAATGAAAGCATTAAGAACACACGTTCTGATTTTGAGAAGGCAGCTTTGGTCCAATTGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

775

Amino Acids

86.21

Weight (kDa)

5.75

Isoelectric Point (pI)

43.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Agenet	PF05641	19 - 87	8.9e-19	Agenet domain
DUF724	PF05266	587 - 770	1.4e-61	Protein of unknown function (DUF724)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	347
AccI	GTMKAC	1 cut(s)	300
AccII	CGCG	3 cut(s)	697, 1875, 2096
AccIII	TCCGGA	1 cut(s)	1592
AciI	CCGC	6 cut(s)	5, 237, 257, 389, 503, 2096
AclWI	GGATC	5 cut(s)	380, 847, 1235, 1420, 2194
AcoI	YGGCCR	1 cut(s)	289
AcsI	RAATTY	1 cut(s)	404
AcuI	CTGAAG	5 cut(s)	471, 564, 1178, 1332, 1482
AcvI	CACGTG	1 cut(s)	265
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	2 cut(s)	179, 649
AfiI	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 556, 680, 1270, 1313, 1785, 1957
AflIII	ACRYGT	1 cut(s)	2285
AhdI	GACNNNNNGTC	1 cut(s)	727
AhlI	ACTAGT	1 cut(s)	611
AjnI	CCWGG	6 cut(s)	815, 945, 1269, 1517, 1540, 2202
AjuI	GAANNNNNNNTTGG	2 cut(s)	2294, 2326
AloI	GAACNNNNNNTCC	2 cut(s)	1023, 1055
Alw21I	GWGCWC	1 cut(s)	1778
Alw26I	GTCTC	4 cut(s)	210, 408, 976, 1080
AlwI	GGATC	5 cut(s)	380, 847, 1235, 1420, 2194
AlwNI	CAGNNNCTG	3 cut(s)	217, 478, 587
Aor13HI	TCCGGA	1 cut(s)	1592
AoxI	GGCC	3 cut(s)	248, 289, 461
ApoI	RAATTY	1 cut(s)	404
Asp700I	GAANNNNTTC	1 cut(s)	919
AspLEI	GCGC	2 cut(s)	673, 967
AspS9I	GGNCC	6 cut(s)	327, 462, 1193, 1575, 1649, 2314
AsuC2I	CCSGG	1 cut(s)	1482
AsuHPI	GGTGA	4 cut(s)	184, 884, 1912, 2008
AsuII	TTCGAA	1 cut(s)	923
AsuNHI	GCTAGC	2 cut(s)	137, 2237
AvaII	GGWCC	5 cut(s)	327, 1193, 1575, 1649, 2314
AxyI	CCTNAGG	1 cut(s)	555
BaeGI	GKGCMC	1 cut(s)	946
BanII	GRGCYC	3 cut(s)	131, 169, 1778
BauI	CACGAG	1 cut(s)	1163
BbrPI	CACGTG	1 cut(s)	265
BbsI	GAAGAC	1 cut(s)	204
Bbv12I	GWGCWC	1 cut(s)	1778
BccI	CCATC	4 cut(s)	8, 438, 558, 863
BceAI	ACGGC	2 cut(s)	304, 773
BcgI	CGANNNNNNTGC	2 cut(s)	1865, 1899
BciT130I	CCWGG	6 cut(s)	817, 947, 1271, 1519, 1542, 2204
BcnI	CCSGG	1 cut(s)	1482
BcoDI	GTCTC	4 cut(s)	210, 408, 976, 1080
BcuI	ACTAGT	1 cut(s)	611
BfaI	CTAG	8 cut(s)	138, 612, 834, 878, 972, 1347, 1572, 2238
BfmI	CTRYAG	4 cut(s)	588, 687, 1083, 1440
BfoI	RGCGCY	1 cut(s)	674
BglII	AGATCT	1 cut(s)	277
BlpI	GCTNAGC	1 cut(s)	142
Bme1390I	CCNGG	7 cut(s)	817, 947, 1271, 1482, 1519, 1542, 2204
Bme18I	GGWCC	5 cut(s)	327, 1193, 1575, 1649, 2314
BmeRI	GACNNNNNGTC	1 cut(s)	727
BmgT120I	GGNCC	6 cut(s)	327, 462, 1193, 1575, 1649, 2314
BmiI	GGNNCC	4 cut(s)	19, 1356, 1577, 1650
BmrFI	CCNGG	7 cut(s)	817, 947, 1271, 1482, 1519, 1542, 2204
BmsI	GCATC	9 cut(s)	520, 666, 882, 1483, 1501, 1598, 2166, 2204, 2248
BmtI	GCTAGC	2 cut(s)	141, 2241
BoxI	GACNNNNGTC	2 cut(s)	188, 696
BpiI	GAAGAC	1 cut(s)	204
BplI	GAGNNNNNCTC	2 cut(s)	757, 789
BpmI	CTGGAG	1 cut(s)	1698
Bpu1102I	GCTNAGC	1 cut(s)	142
Bpu14I	TTCGAA	1 cut(s)	923
BpuEI	CTTGAG	2 cut(s)	915, 2106
BpuMI	CCSGG	1 cut(s)	1482
Bsa29I	ATCGAT	1 cut(s)	2198
BsaAI	YACGTR	2 cut(s)	265, 2027
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	2 cut(s)	210, 408
BsaJI	CCNNGG	5 cut(s)	186, 755, 816, 945, 1848
BsaWI	WCCGGW	2 cut(s)	329, 1592
Bsc4I	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 556, 680, 1270, 1313, 1785, 1957
Bse118I	RCCGGY	1 cut(s)	446
Bse1I	ACTGG	4 cut(s)	893, 1244, 1715, 1911
Bse21I	CCTNAGG	1 cut(s)	555
Bse3DI	GCAATG	1 cut(s)	2232
BseAI	TCCGGA	1 cut(s)	1592
BseBI	CCWGG	6 cut(s)	817, 947, 1271, 1519, 1542, 2204
BseCI	ATCGAT	1 cut(s)	2198
BseDI	CCNNGG	5 cut(s)	186, 755, 816, 945, 1848
BseGI	GGATG	6 cut(s)	550, 670, 897, 1589, 1669, 2263
BseLI	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 556, 680, 1270, 1313, 1785, 1957
BseMI	GCAATG	1 cut(s)	2232
BseMII	CTCAG	4 cut(s)	156, 221, 470, 546
BseNI	ACTGG	4 cut(s)	893, 1244, 1715, 1911
BseRI	GAGGAG	1 cut(s)	88
BseSI	GKGCMC	1 cut(s)	946
BseYI	CCCAGC	2 cut(s)	27, 130
BsgI	GTGCAG	1 cut(s)	549
Bsh1236I	CGCG	3 cut(s)	697, 1875, 2096
BshFI	GGCC	3 cut(s)	250, 291, 463
BshVI	ATCGAT	1 cut(s)	2198
BsiHKAI	GWGCWC	1 cut(s)	1778
BsiSI	CCGG	4 cut(s)	330, 447, 1482, 1593
BslFI	GGGAC	1 cut(s)	1830
BslI	CCNNNNNNNGG	9 cut(s)	201, 369, 453, 556, 680, 1270, 1313, 1785, 1957
BsmAI	GTCTC	4 cut(s)	210, 408, 976, 1080
BsmFI	GGGAC	1 cut(s)	1830
BsmI	GAATGC	2 cut(s)	1189, 1647
BsnI	GGCC	3 cut(s)	250, 291, 463
Bso31I	GGTCTC	2 cut(s)	210, 408
Bsp119I	TTCGAA	1 cut(s)	923
Bsp1286I	GDGCHC	4 cut(s)	131, 169, 946, 1778
Bsp13I	TCCGGA	1 cut(s)	1592
Bsp143I	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
Bsp1720I	GCTNAGC	1 cut(s)	142
Bsp68I	TCGCGA	1 cut(s)	1875
BspACI	CCGC	6 cut(s)	5, 237, 257, 389, 503, 2096
BspANI	GGCC	3 cut(s)	250, 291, 463
BspCNI	CTCAG	4 cut(s)	155, 220, 471, 547
BspDI	ATCGAT	1 cut(s)	2198
BspEI	TCCGGA	1 cut(s)	1592
BspFNI	CGCG	3 cut(s)	697, 1875, 2096
BspLI	GGNNCC	4 cut(s)	19, 1356, 1577, 1650
BspMAI	CTGCAG	1 cut(s)	592
BspOI	GCTAGC	2 cut(s)	141, 2241
BspPI	GGATC	5 cut(s)	380, 847, 1235, 1420, 2194
BspQI	GCTCTTC	2 cut(s)	501, 1766
BspT104I	TTCGAA	1 cut(s)	923
BspTNI	GGTCTC	2 cut(s)	210, 408
BsrDI	GCAATG	1 cut(s)	2232
BsrFI	RCCGGY	1 cut(s)	446
BsrI	ACTGG	4 cut(s)	893, 1244, 1715, 1911
BssAI	RCCGGY	1 cut(s)	446
BssECI	CCNNGG	5 cut(s)	186, 755, 816, 945, 1848
BssMI	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
BssNI	GRCGYC	1 cut(s)	303
BssSI	CACGAG	1 cut(s)	1163
BssT1I	CCWWGG	2 cut(s)	186, 1848
Bst2BI	CACGAG	1 cut(s)	1163
Bst2UI	CCWGG	6 cut(s)	817, 947, 1271, 1519, 1542, 2204
Bst4CI	ACNGT	7 cut(s)	320, 419, 1216, 1681, 1738, 2020, 2073
Bst6I	CTCTTC	3 cut(s)	155, 501, 1766
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	1 cut(s)	517
BstBAI	YACGTR	2 cut(s)	265, 2027
BstBI	TTCGAA	1 cut(s)	923
BstC8I	GCNNGC	6 cut(s)	139, 448, 1698, 2193, 2239, 2243
BstDEI	CTNAG	4 cut(s)	142, 207, 479, 555
BstDSI	CCRYGG	1 cut(s)	755
BstEII	GGTNACC	2 cut(s)	190, 1900
BstF5I	GGATG	6 cut(s)	550, 670, 897, 1589, 1669, 2263
BstFNI	CGCG	3 cut(s)	697, 1875, 2096
BstH2I	RGCGCY	1 cut(s)	674
BstHHI	GCGC	2 cut(s)	673, 967
BstKTI	GATC	8 cut(s)	280, 375, 842, 1174, 1243, 1415, 1450, 2202
BstMAI	GTCTC	4 cut(s)	210, 408, 976, 1080
BstMBI	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
BstMWI	GCNNNNNNNGC	7 cut(s)	143, 256, 469, 517, 523, 526, 950
BstNI	CCWGG	6 cut(s)	817, 947, 1271, 1519, 1542, 2204
BstNSI	RCATGY	1 cut(s)	2195
BstPAI	GACNNNNGTC	2 cut(s)	188, 696
BstPI	GGTNACC	2 cut(s)	190, 1900
BstSCI	CCNGG	7 cut(s)	815, 945, 1269, 1480, 1517, 1540, 2202
BstSFI	CTRYAG	4 cut(s)	588, 687, 1083, 1440
BstSLI	GKGCMC	1 cut(s)	946
BstUI	CGCG	3 cut(s)	697, 1875, 2096
BstV2I	GAAGAC	1 cut(s)	204
BstX2I	RGATCY	2 cut(s)	277, 372
BstXI	CCANNNNNNTGG	1 cut(s)	1918
BstYI	RGATCY	2 cut(s)	277, 372
Bsu15I	ATCGAT	1 cut(s)	2198
Bsu36I	CCTNAGG	1 cut(s)	555
BsuRI	GGCC	3 cut(s)	250, 291, 463
BsuTUI	ATCGAT	1 cut(s)	2198
BtgI	CCRYGG	1 cut(s)	755
BtsCI	GGATG	6 cut(s)	550, 670, 897, 1589, 1669, 2263
BtsI	GCAGTG	3 cut(s)	807, 832, 1243
BtsIMutI	CAGTG	7 cut(s)	215, 807, 832, 886, 1243, 1734, 1735
BtuMI	TCGCGA	1 cut(s)	1875
Cac8I	GCNNGC	6 cut(s)	139, 448, 1698, 2193, 2239, 2243
CaiI	CAGNNNCTG	3 cut(s)	217, 478, 587
CfoI	GCGC	2 cut(s)	673, 967
Cfr10I	RCCGGY	1 cut(s)	446
Cfr13I	GGNCC	6 cut(s)	327, 462, 1193, 1575, 1649, 2314
ClaI	ATCGAT	1 cut(s)	2198
CseI	GACGC	4 cut(s)	311, 686, 1503, 2102
Csp6I	GTAC	2 cut(s)	178, 648
CspCI	CAANNNNNGTGG	4 cut(s)	744, 779, 1615, 1650
CviAII	CATG	6 cut(s)	1131, 1232, 1288, 1629, 1660, 2192
CviQI	GTAC	2 cut(s)	178, 648
DdeI	CTNAG	4 cut(s)	142, 207, 479, 555
DpnI	GATC	8 cut(s)	279, 374, 841, 1173, 1242, 1414, 1449, 2201
DpnII	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
DrdI	GACNNNNNNGTC	1 cut(s)	347
DriI	GACNNNNNGTC	1 cut(s)	727
DseDI	GACNNNNNNGTC	1 cut(s)	347
EaeI	YGGCCR	1 cut(s)	289
Eam1104I	CTCTTC	3 cut(s)	155, 501, 1766
Eam1105I	GACNNNNNGTC	1 cut(s)	727
EarI	CTCTTC	3 cut(s)	155, 501, 1766
Ecl136II	GAGCTC	1 cut(s)	1776
Eco130I	CCWWGG	2 cut(s)	186, 1848
Eco147I	AGGCCT	1 cut(s)	250
Eco24I	GRGCYC	3 cut(s)	131, 169, 1778
Eco31I	GGTCTC	2 cut(s)	210, 408
Eco32I	GATATC	1 cut(s)	1929
Eco47I	GGWCC	5 cut(s)	327, 1193, 1575, 1649, 2314
Eco53kI	GAGCTC	1 cut(s)	1776
Eco57I	CTGAAG	5 cut(s)	471, 564, 1178, 1332, 1482
Eco72I	CACGTG	1 cut(s)	265
Eco81I	CCTNAGG	1 cut(s)	555
Eco91I	GGTNACC	2 cut(s)	190, 1900
EcoICRI	GAGCTC	1 cut(s)	1776
EcoO109I	RGGNCCY	1 cut(s)	1575
EcoO65I	GGTNACC	2 cut(s)	190, 1900
EcoRI	GAATTC	1 cut(s)	404
EcoRII	CCWGG	6 cut(s)	815, 945, 1269, 1517, 1540, 2202
EcoRV	GATATC	1 cut(s)	1929
EcoT14I	CCWWGG	2 cut(s)	186, 1848
EcoT22I	ATGCAT	1 cut(s)	2197
EcoT38I	GRGCYC	3 cut(s)	131, 169, 1778
ErhI	CCWWGG	2 cut(s)	186, 1848
FaeI	CATG	6 cut(s)	1134, 1235, 1291, 1632, 1663, 2195
FalI	AAGNNNNNCTT	2 cut(s)	1680, 1712
FaqI	GGGAC	1 cut(s)	1830
FatI	CATG	6 cut(s)	1130, 1231, 1287, 1628, 1659, 2191
FauI	CCCGC	1 cut(s)	396
FblI	GTMKAC	1 cut(s)	300
FokI	GGATG	6 cut(s)	537, 677, 904, 1576, 1676, 2270
FriOI	GRGCYC	3 cut(s)	131, 169, 1778
FspBI	CTAG	8 cut(s)	138, 612, 834, 878, 972, 1347, 1572, 2238
GlaI	GCGC	2 cut(s)	672, 966
GsaI	CCCAGC	2 cut(s)	31, 134
GsuI	CTGGAG	1 cut(s)	1698
HaeII	RGCGCY	1 cut(s)	674
HaeIII	GGCC	3 cut(s)	250, 291, 463
HapII	CCGG	4 cut(s)	330, 447, 1482, 1593
HgaI	GACGC	4 cut(s)	311, 686, 1503, 2102
HhaI	GCGC	2 cut(s)	673, 967
Hin1I	GRCGYC	1 cut(s)	303
Hin1II	CATG	6 cut(s)	1134, 1235, 1291, 1632, 1663, 2195
Hin6I	GCGC	2 cut(s)	671, 965
HinP1I	GCGC	2 cut(s)	671, 965
HincII	GTYRAC	2 cut(s)	301, 1429
HindII	GTYRAC	2 cut(s)	301, 1429
HindIII	AAGCTT	1 cut(s)	1694
HpaII	CCGG	4 cut(s)	330, 447, 1482, 1593
HphI	GGTGA	4 cut(s)	184, 884, 1912, 2008
Hpy166II	GTNNAC	4 cut(s)	301, 327, 1429, 2038
Hpy8I	GTNNAC	4 cut(s)	301, 327, 1429, 2038
Hpy99I	CGWCG	2 cut(s)	83, 305
HpyAV	CCTTC	6 cut(s)	909, 919, 998, 1268, 1307, 2296
HpyCH4III	ACNGT	7 cut(s)	320, 419, 1216, 1681, 1738, 2020, 2073
HpyCH4IV	ACGT	4 cut(s)	264, 1809, 2026, 2287
HpyF10VI	GCNNNNNNNGC	7 cut(s)	143, 256, 469, 517, 523, 526, 950
HpyF3I	CTNAG	4 cut(s)	142, 207, 479, 555
HpySE526I	ACGT	4 cut(s)	264, 1809, 2026, 2287
Hsp92I	GRCGYC	1 cut(s)	303
Hsp92II	CATG	6 cut(s)	1134, 1235, 1291, 1632, 1663, 2195
HspAI	GCGC	2 cut(s)	671, 965
Kpn2I	TCCGGA	1 cut(s)	1592
KroI	GCCGGC	1 cut(s)	446
KroNI	GCCGGC	1 cut(s)	448
Kzo9I	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
LguI	GCTCTTC	2 cut(s)	501, 1766
LmnI	GCTCC	6 cut(s)	23, 75, 98, 634, 1781, 2113
LweI	GCATC	9 cut(s)	520, 666, 882, 1483, 1501, 1598, 2166, 2204, 2248
MaeI	CTAG	8 cut(s)	138, 612, 834, 878, 972, 1347, 1572, 2238
MaeII	ACGT	4 cut(s)	264, 1809, 2026, 2287
MaeIII	GTNAC	7 cut(s)	190, 748, 1216, 1730, 1900, 2014, 2228
MalI	GATC	8 cut(s)	279, 374, 841, 1173, 1242, 1414, 1449, 2201
MboI	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
MfeI	CAATTG	3 cut(s)	761, 1794, 2318
MflI	RGATCY	2 cut(s)	277, 372
MhlI	GDGCHC	4 cut(s)	131, 169, 946, 1778
MluCI	AATT	9 cut(s)	404, 761, 800, 1070, 1667, 1755, 1794, 2172, 2318
MlyI	GAGTC	7 cut(s)	420, 686, 1097, 1404, 1942, 1990, 2260
MmeI	TCCRAC	2 cut(s)	570, 916
Mph1103I	ATGCAT	1 cut(s)	2197
MroI	TCCGGA	1 cut(s)	1592
MroNI	GCCGGC	1 cut(s)	446
MroXI	GAANNNNTTC	1 cut(s)	919
MseI	TTAA	8 cut(s)	713, 1098, 1296, 1670, 1692, 1854, 2180, 2277
MslI	CAYNNNNRTG	2 cut(s)	1236, 1944
MspA1I	CMGCKG	4 cut(s)	134, 1121, 1226, 1532
MspI	CCGG	4 cut(s)	330, 447, 1482, 1593
MspR9I	CCNGG	7 cut(s)	817, 947, 1271, 1482, 1519, 1542, 2204
MunI	CAATTG	3 cut(s)	761, 1794, 2318
Mva1269I	GAATGC	2 cut(s)	1189, 1647
MvaI	CCWGG	6 cut(s)	817, 947, 1271, 1519, 1542, 2204
MvnI	CGCG	3 cut(s)	697, 1875, 2096
MwoI	GCNNNNNNNGC	7 cut(s)	143, 256, 469, 517, 523, 526, 950
NaeI	GCCGGC	1 cut(s)	448
NciI	CCSGG	1 cut(s)	1482
NdeII	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
NgoMIV	GCCGGC	1 cut(s)	446
NheI	GCTAGC	2 cut(s)	137, 2237
NlaIII	CATG	6 cut(s)	1134, 1235, 1291, 1632, 1663, 2195
NlaIV	GGNNCC	4 cut(s)	19, 1356, 1577, 1650
NmuCI	GTSAC	5 cut(s)	190, 1730, 1900, 2014, 2228
NruI	TCGCGA	1 cut(s)	1875
NsiI	ATGCAT	1 cut(s)	2197
NspI	RCATGY	1 cut(s)	2195
NspV	TTCGAA	1 cut(s)	923
PaeI	GCATGC	1 cut(s)	2195
PceI	AGGCCT	1 cut(s)	250
PciSI	GCTCTTC	2 cut(s)	501, 1766
PcsI	WCGNNNNNNNCGW	1 cut(s)	18
PctI	GAATGC	2 cut(s)	1189, 1647
PdiI	GCCGGC	1 cut(s)	448
PdmI	GAANNNNTTC	1 cut(s)	919
PfoI	TCCNGGA	3 cut(s)	1480, 1517, 1540
PleI	GAGTC	7 cut(s)	420, 686, 1096, 1403, 1941, 1989, 2259
PmaCI	CACGTG	1 cut(s)	265
PmlI	CACGTG	1 cut(s)	265
PpsI	GAGTC	7 cut(s)	420, 686, 1096, 1403, 1941, 1989, 2259
Ppu21I	YACGTR	2 cut(s)	265, 2027
PpuMI	RGGWCCY	1 cut(s)	1575
PshAI	GACNNNNGTC	2 cut(s)	188, 696
Psp124BI	GAGCTC	1 cut(s)	1778
Psp5II	RGGWCCY	1 cut(s)	1575
Psp6I	CCWGG	6 cut(s)	815, 945, 1269, 1517, 1540, 2202
PspCI	CACGTG	1 cut(s)	265
PspEI	GGTNACC	2 cut(s)	190, 1900
PspFI	CCCAGC	2 cut(s)	27, 130
PspGI	CCWGG	6 cut(s)	815, 945, 1269, 1517, 1540, 2202
PspN4I	GGNNCC	4 cut(s)	19, 1356, 1577, 1650
PspPI	GGNCC	6 cut(s)	327, 462, 1193, 1575, 1649, 2314
PspPPI	RGGWCCY	1 cut(s)	1575
PstI	CTGCAG	1 cut(s)	592
PstNI	CAGNNNCTG	3 cut(s)	217, 478, 587
PsuI	RGATCY	2 cut(s)	277, 372
PvuII	CAGCTG	4 cut(s)	134, 1121, 1226, 1532
RruI	TCGCGA	1 cut(s)	1875
RsaI	GTAC	2 cut(s)	179, 649
RsaNI	GTAC	2 cut(s)	178, 648
RseI	CAYNNNNRTG	2 cut(s)	1236, 1944
SacI	GAGCTC	1 cut(s)	1778
SalI	GTCGAC	1 cut(s)	299
SapI	GCTCTTC	2 cut(s)	501, 1766
SaqAI	TTAA	8 cut(s)	713, 1098, 1296, 1670, 1692, 1854, 2180, 2277
Sau3AI	GATC	8 cut(s)	277, 372, 839, 1171, 1240, 1412, 1447, 2199
Sau96I	GGNCC	6 cut(s)	327, 462, 1193, 1575, 1649, 2314
SchI	GAGTC	7 cut(s)	420, 686, 1097, 1404, 1942, 1990, 2260
ScrFI	CCNGG	7 cut(s)	817, 947, 1271, 1482, 1519, 1542, 2204
SduI	GDGCHC	4 cut(s)	131, 169, 946, 1778
SfaNI	GCATC	9 cut(s)	520, 666, 882, 1483, 1501, 1598, 2166, 2204, 2248
SfcI	CTRYAG	4 cut(s)	588, 687, 1083, 1440
SfuI	TTCGAA	1 cut(s)	923
SinI	GGWCC	5 cut(s)	327, 1193, 1575, 1649, 2314
SmiMI	CAYNNNNRTG	2 cut(s)	1236, 1944
SmlI	CTYRAG	2 cut(s)	930, 2121
SmoI	CTYRAG	2 cut(s)	930, 2121
SpeI	ACTAGT	1 cut(s)	611
SphI	GCATGC	1 cut(s)	2195
Sse9I	AATT	9 cut(s)	404, 761, 800, 1070, 1667, 1755, 1794, 2172, 2318
SseBI	AGGCCT	1 cut(s)	250
SsiI	CCGC	6 cut(s)	5, 237, 257, 389, 503, 2096
SspMI	CTAG	8 cut(s)	138, 612, 834, 878, 972, 1347, 1572, 2238
SstI	GAGCTC	1 cut(s)	1778
StuI	AGGCCT	1 cut(s)	250
StyD4I	CCNGG	7 cut(s)	815, 945, 1269, 1480, 1517, 1540, 2202
StyI	CCWWGG	2 cut(s)	186, 1848
TaaI	ACNGT	7 cut(s)	320, 419, 1216, 1681, 1738, 2020, 2073
TaiI	ACGT	4 cut(s)	267, 1812, 2029, 2290
TaqI	TCGA	8 cut(s)	12, 231, 300, 408, 923, 1931, 2160, 2198
TasI	AATT	9 cut(s)	404, 761, 800, 1070, 1667, 1755, 1794, 2172, 2318
TatI	WGTACW	1 cut(s)	177
TauI	GCSGC	1 cut(s)	259
Tru1I	TTAA	8 cut(s)	713, 1098, 1296, 1670, 1692, 1854, 2180, 2277
Tru9I	TTAA	8 cut(s)	713, 1098, 1296, 1670, 1692, 1854, 2180, 2277
TscAI	CASTG	7 cut(s)	222, 814, 832, 893, 1243, 1735, 1741
TseFI	GTSAC	5 cut(s)	190, 1730, 1900, 2014, 2228
Tsp45I	GTSAC	5 cut(s)	190, 1730, 1900, 2014, 2228
TspDTI	ATGAA	5 cut(s)	1104, 1476, 1578, 1680, 2283
TspGWI	ACGGA	1 cut(s)	1253
TspRI	CASTG	7 cut(s)	222, 814, 832, 893, 1243, 1735, 1741
VpaK11BI	GGWCC	5 cut(s)	327, 1193, 1575, 1649, 2314
XapI	RAATTY	1 cut(s)	404
XceI	RCATGY	1 cut(s)	2195
XmiI	GTMKAC	1 cut(s)	300
XmnI	GAANNNNTTC	1 cut(s)	919
XspI	CTAG	8 cut(s)	138, 612, 834, 878, 972, 1347, 1572, 2238
Zsp2I	ATGCAT	1 cut(s)	2197

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.