Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

18235804 .. 18241610

Gene Structure 5807 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00347050.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGACCCCTCCAACGCCTCCGCCGAGGTCACCTCCGTCATTCTCGACAATCGCGACTTCGTTATGATCCTCACGACCTCGATTGCCGTTTTGATCGGCTGCGTCGTCGTTTTTGTGTGGCGGCGATCGAGCGCTCAGAAGCCCAGGGTCGTCCAGCCTCTTAAGCCGTTGATCATCGCGGAGCCGGAGCCGGAGCTCGACGATGGAAGTAAGAAGGTCACAATCTTCTTCGGCACGCAAACCGGTACTGCTGAGGGATTTGCCAAGGCTTTTGCTGAAGAAGCGAAAGCTCGCTATGAAAAGGTCACATTCAAAGTTGTTGATTTGGACGATTATGCAGCTGATGACGAGGCGTATGAGGAGAAATTGAAAAATGAGAGTCTGGCAATTTTCTTTTTTGGCGACGGTAAAGAGAAAGAAGCATGGCTTCAAAATCTTCATTACGGAGTGTTTGGTCTCGGAAACAGGCAGTATGAGCATTTCAACAAGGTGGCCGTGGTTGTGGATGAGTTCCTCGCTGCTCAGGGTGGGAAGCGACTTGTTCCAGTGGGCCTCGGAGATGACGATCAATGCATAGAGGATGATTTCACTGCATGGCGAGAGTTGATGTGGCCTGAGTTGGACCAGTTGCTTCGAGATGAGGATGATGCAACCACACCAGCTTGCACACCTTACACTGCTGCTGTCTTGGAGTATCGAGTTGTATTTCATGATGCTGCTGATGTATCAGTAAAGGATAAGAGCTGGAATAATGCAAATGGTCATGCTGTTATTGATGCTCAGCATCCATGCAGGGCTAATGTTGCTGTGAGAAAGGAACTTCATACTCCTGCATCTGATCGTTCTTGCACCCATCTCGAATTTGATATTGCTGGCACTGGACTTACATACGAAACTGGTGATCATGTCGGTGTGTACTGTGAGAATCTTTCTGAAACTGTGGAAGAGGCGATAAGTTTGTTGGGCTTGGCTCCGGAAACATACTTCTCGTTGCACACTGATAAGGATGATGGTACACCACTTAGCGGAGGCTCCTTGTTACCTCCCTTTCCACCGTGCACATTAAGAACAGCACTAACTCGATATGCTGACCTTTTGAGTTCTCCGAAAAAGTCTGCCCTGCTGGCTTTAGCTGCCTATGCATCTGATCCAGCTGAAGCGGATCGATTAAGACATCTTTCGTCACCTGCTGGGAAGGATGAATATGCTGAGTGGATGGTTGCGAGTCAGAGAAGCCTTCTGGAGGTCATGGCTGAATTTCCATCAGCCAAGCCCCAACTTGGAGTCTTCTTTGCAGCAGTTGCTCCAAGGTTGCAGCCTAGATACTATTCAATCTCGTCATCCCCGAGGATGGCACCATCTAGAATTCATGTTACTTGTGCACTAGTTTATGACAAAACATCAACAGGAAGGATTCACAAAGGAGTGTGTTCAACCTGGATGAAGAATTCTGTGCCTTCCGCAAAAAGTGATGATTGTAGTTGGGCACCTGTTTTTGTTCGGCAATCAAACTTCAGACTTCCCGTTGATACTAAAGTACCTATTCTTATGATTGGTCCTGGAACTGGATTGGCTCCTTTCAGGGGTTTCCTGCAGGACTACATTTATGAAGATGAGCTGAACAACTTTGTAGAAAAAGGTGCACTTTCTGAGCTTGTGGTTGCATTGTCCCGCCAGGGACCCACCAAGGAATATGTGCAACATAAAATGATGCAGAAGGCTTCAGACATCTGGAATATGCTATCTCAGGGAGGTTATATTTACGTTTGTGGTGATGCTAAGGGCATGGCCAGGGATGTCCACCGGGACCCTTCACACAATCGTGCAGGAGCAGGTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

613

Amino Acids

67.57

Weight (kDa)

5.19

Isoelectric Point (pI)

38.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	75 - 199	2.6e-18	Flavodoxin
FAD_binding_1	PF00667	259 - 480	9e-71	FAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1195
Acc36I	ACCTGC	2 cut(s)	1195, 1823
AccB1I	GGYRCC	2 cut(s)	1354, 1486
AccII	CGCG	2 cut(s)	54, 179
AccIII	TCCGGA	1 cut(s)	973
AciI	CCGC	7 cut(s)	21, 121, 179, 1026, 1160, 1461, 1672
AclWI	GGATC	3 cut(s)	61, 1142, 1170
AcoI	YGGCCR	2 cut(s)	492, 1788
AcsI	RAATTY	4 cut(s)	860, 1256, 1365, 1447
AcuI	CTGAAG	4 cut(s)	297, 1176, 1498, 1707
AfaI	GTAC	4 cut(s)	247, 917, 1015, 1539
AfeI	AGCGCT	1 cut(s)	133
AfiI	CCNNNNNNNGG	6 cut(s)	1025, 1243, 1280, 1351, 1565, 1583
AflII	CTTAAG	1 cut(s)	161
AgeI	ACCGGT	1 cut(s)	242
AgsI	TTSAA	6 cut(s)	313, 370, 431, 484, 1332, 1434
AhlI	ACTAGT	1 cut(s)	1384
AjnI	CCWGG	5 cut(s)	143, 1436, 1558, 1674, 1790
AleI	CACNNNNGTG	1 cut(s)	1821
AluBI	AGCT	9 cut(s)	196, 290, 341, 662, 744, 1133, 1154, 1618, 1654
AluI	AGCT	9 cut(s)	196, 290, 341, 662, 744, 1133, 1154, 1618, 1654
Alw21I	GWGCWC	4 cut(s)	198, 1061, 1384, 1645
Alw26I	GTCTC	1 cut(s)	461
Alw44I	GTGCAC	3 cut(s)	1057, 1380, 1641
AlwI	GGATC	3 cut(s)	61, 1142, 1170
Ama87I	CYCGRG	1 cut(s)	1345
Aor13HI	TCCGGA	1 cut(s)	973
Aor51HI	AGCGCT	1 cut(s)	133
AoxI	GGCC	4 cut(s)	492, 550, 611, 1788
ApaLI	GTGCAC	3 cut(s)	1057, 1380, 1641
ApeKI	GCWGC	8 cut(s)	99, 338, 518, 680, 716, 1133, 1295, 1315
ApoI	RAATTY	4 cut(s)	860, 1256, 1365, 1447
AsiGI	ACCGGT	1 cut(s)	242
AspLEI	GCGC	1 cut(s)	134
AspS9I	GGNCC	6 cut(s)	4, 550, 622, 1556, 1679, 1807
AsuC2I	CCSGG	1 cut(s)	1805
AsuHPI	GGTGA	4 cut(s)	22, 911, 1176, 1784
AvaI	CYCGRG	1 cut(s)	1345
AvaII	GGWCC	5 cut(s)	4, 622, 1556, 1679, 1807
BaeGI	GKGCMC	4 cut(s)	1061, 1384, 1489, 1645
BaeI	ACNNNNGTAYC	4 cut(s)	1521, 1521, 1554, 1554
BalI	TGGCCA	1 cut(s)	1790
BanI	GGYRCC	2 cut(s)	1354, 1486
BanII	GRGCYC	1 cut(s)	198
BbsI	GAAGAC	1 cut(s)	1279
Bbv12I	GWGCWC	4 cut(s)	198, 1061, 1384, 1645
BbvCI	CCTCAGC	1 cut(s)	252
BbvI	GCAGC	8 cut(s)	86, 350, 505, 667, 703, 1120, 1307, 1327
BccI	CCATC	7 cut(s)	197, 861, 1004, 1210, 1270, 1345, 1366
BceAI	ACGGC	3 cut(s)	71, 151, 479
BcgI	CGANNNNNNTGC	2 cut(s)	838, 872
BciT130I	CCWGG	5 cut(s)	145, 1438, 1560, 1676, 1792
BclI	TGATCA	2 cut(s)	171, 901
BcnI	CCSGG	1 cut(s)	1805
BcoDI	GTCTC	1 cut(s)	461
BcuI	ACTAGT	1 cut(s)	1384
BfaI	CTAG	3 cut(s)	1320, 1362, 1385
BfmI	CTRYAG	1 cut(s)	1592
BfoI	RGCGCY	1 cut(s)	135
BfrI	CTTAAG	1 cut(s)	161
BfuAI	ACCTGC	2 cut(s)	1195, 1823
BglI	GCCNNNNNGGC	1 cut(s)	1124
BisI	GCNGC	9 cut(s)	100, 122, 339, 519, 681, 717, 1134, 1296, 1316
BlpI	GCTNAGC	1 cut(s)	780
BlsI	GCNGC	9 cut(s)	101, 123, 340, 520, 682, 718, 1135, 1297, 1317
Bme1390I	CCNGG	6 cut(s)	145, 1438, 1560, 1676, 1792, 1805
Bme18I	GGWCC	5 cut(s)	4, 622, 1556, 1679, 1807
BmeT110I	CYCGRG	1 cut(s)	1345
BmgT120I	GGNCC	6 cut(s)	4, 550, 622, 1556, 1679, 1807
BmrFI	CCNGG	6 cut(s)	145, 1438, 1560, 1676, 1792, 1805
BmsI	GCATC	8 cut(s)	637, 703, 766, 793, 842, 1151, 1701, 1765
BpiI	GAAGAC	1 cut(s)	1279
BplI	GAGNNNNNCTC	2 cut(s)	17, 49
BpmI	CTGGAG	1 cut(s)	1262
Bpu10I	CCTNAGC	3 cut(s)	252, 522, 1779
Bpu1102I	GCTNAGC	1 cut(s)	780
BpuMI	CCSGG	1 cut(s)	1805
Bsa29I	ATCGAT	1 cut(s)	1165
BsaBI	GATNNNNATC	1 cut(s)	564
BsaI	GGTCTC	1 cut(s)	461
BsaWI	WCCGGW	2 cut(s)	242, 973
Bsc4I	CCNNNNNNNGG	6 cut(s)	1025, 1243, 1280, 1351, 1565, 1583
Bse118I	RCCGGY	1 cut(s)	242
Bse1I	ACTGG	5 cut(s)	545, 625, 883, 901, 1570
Bse8I	GATNNNNATC	1 cut(s)	564
BseAI	TCCGGA	1 cut(s)	973
BseBI	CCWGG	5 cut(s)	145, 1438, 1560, 1676, 1792
BseCI	ATCGAT	1 cut(s)	1165
BseJI	GATNNNNATC	1 cut(s)	564
BseLI	CCNNNNNNNGG	6 cut(s)	1025, 1243, 1280, 1351, 1565, 1583
BseMII	CTCAG	8 cut(s)	149, 243, 536, 606, 794, 1200, 1641, 1760
BseNI	ACTGG	5 cut(s)	545, 625, 883, 901, 1570
BseRI	GAGGAG	1 cut(s)	374
BseSI	GKGCMC	4 cut(s)	1061, 1384, 1489, 1645
BseXI	GCAGC	8 cut(s)	86, 350, 505, 667, 703, 1120, 1307, 1327
BseYI	CCCAGC	1 cut(s)	1190
Bsh1236I	CGCG	2 cut(s)	54, 179
Bsh1285I	CGRYCG	1 cut(s)	128
BshFI	GGCC	4 cut(s)	494, 552, 613, 1790
BshNI	GGYRCC	2 cut(s)	1354, 1486
BshTI	ACCGGT	1 cut(s)	242
BshVI	ATCGAT	1 cut(s)	1165
BsiEI	CGRYCG	1 cut(s)	128
BsiHKAI	GWGCWC	4 cut(s)	198, 1061, 1384, 1645
BsiHKCI	CYCGRG	1 cut(s)	1345
BsiSI	CCGG	5 cut(s)	185, 191, 243, 974, 1804
BslFI	GGGAC	3 cut(s)	1654, 1692, 1820
BslI	CCNNNNNNNGG	6 cut(s)	1025, 1243, 1280, 1351, 1565, 1583
BsmAI	GTCTC	1 cut(s)	461
BsmFI	GGGAC	3 cut(s)	1654, 1692, 1820
BsnI	GGCC	4 cut(s)	494, 552, 613, 1790
Bso31I	GGTCTC	1 cut(s)	461
BsoBI	CYCGRG	1 cut(s)	1345
Bsp1286I	GDGCHC	5 cut(s)	198, 1061, 1384, 1489, 1645
Bsp13I	TCCGGA	1 cut(s)	973
Bsp143I	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
Bsp1720I	GCTNAGC	1 cut(s)	780
Bsp68I	TCGCGA	1 cut(s)	54
BspACI	CCGC	7 cut(s)	21, 121, 179, 1026, 1160, 1461, 1672
BspANI	GGCC	4 cut(s)	494, 552, 613, 1790
BspCNI	CTCAG	8 cut(s)	148, 244, 535, 607, 793, 1201, 1642, 1759
BspDI	ATCGAT	1 cut(s)	1165
BspEI	TCCGGA	1 cut(s)	973
BspFNI	CGCG	2 cut(s)	54, 179
BspHI	TCATGA	1 cut(s)	709
BspMAI	CTGCAG	1 cut(s)	1596
BspMI	ACCTGC	2 cut(s)	1195, 1823
BspPI	GGATC	3 cut(s)	61, 1142, 1170
BspT107I	GGYRCC	2 cut(s)	1354, 1486
BspTI	CTTAAG	1 cut(s)	161
BspTNI	GGTCTC	1 cut(s)	461
BsrFI	RCCGGY	1 cut(s)	242
BsrI	ACTGG	5 cut(s)	545, 625, 883, 901, 1570
BssAI	RCCGGY	1 cut(s)	242
BssMI	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
BssT1I	CCWWGG	3 cut(s)	264, 1307, 1686
Bst2UI	CCWGG	5 cut(s)	145, 1438, 1560, 1676, 1792
Bst4CI	ACNGT	4 cut(s)	407, 920, 940, 1056
Bst6I	CTCTTC	1 cut(s)	939
BstAFI	CTTAAG	1 cut(s)	161
BstAPI	GCANNNNNTGC	1 cut(s)	1301
BstC8I	GCNNGC	5 cut(s)	236, 292, 664, 874, 1125
BstDSI	CCRYGG	1 cut(s)	495
BstEII	GGTNACC	1 cut(s)	28
BstENI	CCTNNNNNAGG	1 cut(s)	1241
BstFNI	CGCG	2 cut(s)	54, 179
BstH2I	RGCGCY	1 cut(s)	135
BstHHI	GCGC	1 cut(s)	134
BstKTI	GATC	9 cut(s)	69, 96, 128, 174, 568, 841, 904, 1150, 1165
BstMAI	GTCTC	1 cut(s)	461
BstMBI	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
BstMCI	CGRYCG	1 cut(s)	128
BstMWI	GCNNNNNNNGC	8 cut(s)	163, 281, 475, 803, 1124, 1133, 1139, 1301
BstNI	CCWGG	5 cut(s)	145, 1438, 1560, 1676, 1792
BstPI	GGTNACC	1 cut(s)	28
BstSCI	CCNGG	6 cut(s)	143, 1436, 1558, 1674, 1790, 1803
BstSFI	CTRYAG	1 cut(s)	1592
BstSLI	GKGCMC	4 cut(s)	1061, 1384, 1489, 1645
BstUI	CGCG	2 cut(s)	54, 179
BstV1I	GCAGC	8 cut(s)	86, 350, 505, 667, 703, 1120, 1307, 1327
BstV2I	GAAGAC	1 cut(s)	1279
Bsu15I	ATCGAT	1 cut(s)	1165
BsuRI	GGCC	4 cut(s)	494, 552, 613, 1790
BsuTUI	ATCGAT	1 cut(s)	1165
BtgI	CCRYGG	1 cut(s)	495
BtgZI	GCGATG	1 cut(s)	160
BtsI	GCAGTG	2 cut(s)	588, 675
BtsIMutI	CAGTG	5 cut(s)	552, 588, 675, 876, 996
BtuMI	TCGCGA	1 cut(s)	54
BveI	ACCTGC	2 cut(s)	1195, 1823
Cac8I	GCNNGC	5 cut(s)	236, 292, 664, 874, 1125
CciI	TCATGA	1 cut(s)	709
CfoI	GCGC	1 cut(s)	134
Cfr10I	RCCGGY	1 cut(s)	242
Cfr13I	GGNCC	6 cut(s)	4, 550, 622, 1556, 1679, 1807
ClaI	ATCGAT	1 cut(s)	1165
CseI	GACGC	1 cut(s)	91
Csp6I	GTAC	4 cut(s)	246, 916, 1014, 1538
CspAI	ACCGGT	1 cut(s)	242
CviAII	CATG	9 cut(s)	423, 594, 710, 764, 789, 905, 1249, 1370, 1786
CviQI	GTAC	4 cut(s)	246, 916, 1014, 1538
DpnI	GATC	9 cut(s)	68, 95, 127, 173, 567, 840, 903, 1149, 1164
DpnII	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
EaeI	YGGCCR	2 cut(s)	492, 1788
Eam1104I	CTCTTC	1 cut(s)	939
EarI	CTCTTC	1 cut(s)	939
EciI	GGCGGA	1 cut(s)	10
Ecl136II	GAGCTC	1 cut(s)	196
Eco130I	CCWWGG	3 cut(s)	264, 1307, 1686
Eco24I	GRGCYC	1 cut(s)	198
Eco31I	GGTCTC	1 cut(s)	461
Eco47I	GGWCC	5 cut(s)	4, 622, 1556, 1679, 1807
Eco47III	AGCGCT	1 cut(s)	133
Eco53kI	GAGCTC	1 cut(s)	196
Eco57I	CTGAAG	4 cut(s)	297, 1176, 1498, 1707
Eco88I	CYCGRG	1 cut(s)	1345
Eco91I	GGTNACC	1 cut(s)	28
EcoICRI	GAGCTC	1 cut(s)	196
EcoNI	CCTNNNNNAGG	1 cut(s)	1241
EcoO109I	RGGNCCY	2 cut(s)	1679, 1807
EcoO65I	GGTNACC	1 cut(s)	28
EcoRI	GAATTC	2 cut(s)	1365, 1447
EcoRII	CCWGG	5 cut(s)	143, 1436, 1558, 1674, 1790
EcoT14I	CCWWGG	3 cut(s)	264, 1307, 1686
EcoT22I	ATGCAT	2 cut(s)	575, 1144
EcoT38I	GRGCYC	1 cut(s)	198
ErhI	CCWWGG	3 cut(s)	264, 1307, 1686
FaeI	CATG	9 cut(s)	426, 597, 713, 767, 792, 908, 1252, 1373, 1789
FalI	AAGNNNNNCTT	4 cut(s)	411, 443, 1629, 1661
FaqI	GGGAC	3 cut(s)	1654, 1692, 1820
FatI	CATG	9 cut(s)	422, 593, 709, 763, 788, 904, 1248, 1369, 1785
FauI	CCCGC	1 cut(s)	1679
FbaI	TGATCA	2 cut(s)	171, 901
Fnu4HI	GCNGC	9 cut(s)	100, 122, 339, 519, 681, 717, 1134, 1296, 1316
FriOI	GRGCYC	1 cut(s)	198
Fsp4HI	GCNGC	9 cut(s)	100, 122, 339, 519, 681, 717, 1134, 1296, 1316
FspBI	CTAG	3 cut(s)	1320, 1362, 1385
GlaI	GCGC	1 cut(s)	133
GluI	GCNGC	9 cut(s)	100, 122, 339, 519, 681, 717, 1134, 1296, 1316
GsaI	CCCAGC	1 cut(s)	1194
GsuI	CTGGAG	1 cut(s)	1262
HaeII	RGCGCY	1 cut(s)	135
HaeIII	GGCC	4 cut(s)	494, 552, 613, 1790
HapII	CCGG	5 cut(s)	185, 191, 243, 974, 1804
HgaI	GACGC	1 cut(s)	91
HhaI	GCGC	1 cut(s)	134
Hin1II	CATG	9 cut(s)	426, 597, 713, 767, 792, 908, 1252, 1373, 1789
Hin6I	GCGC	1 cut(s)	132
HinP1I	GCGC	1 cut(s)	132
HinfI	GANTC	5 cut(s)	379, 925, 1225, 1284, 1414
HpaII	CCGG	5 cut(s)	185, 191, 243, 974, 1804
HphI	GGTGA	4 cut(s)	22, 911, 1176, 1784
Hpy166II	GTNNAC	6 cut(s)	916, 1016, 1059, 1382, 1643, 1801
Hpy8I	GTNNAC	6 cut(s)	916, 1016, 1059, 1382, 1643, 1801
Hpy99I	CGWCG	4 cut(s)	107, 110, 203, 407
HpyAV	CCTTC	7 cut(s)	208, 1189, 1247, 1404, 1467, 1711, 1821
HpyCH4III	ACNGT	4 cut(s)	407, 920, 940, 1056
HpyCH4IV	ACGT	1 cut(s)	1764
HpyF10VI	GCNNNNNNNGC	8 cut(s)	163, 281, 475, 803, 1124, 1133, 1139, 1301
HpySE526I	ACGT	1 cut(s)	1764
Hsp92II	CATG	9 cut(s)	426, 597, 713, 767, 792, 908, 1252, 1373, 1789
HspAI	GCGC	1 cut(s)	132
KflI	GGGWCCC	2 cut(s)	1679, 1807
Kpn2I	TCCGGA	1 cut(s)	973
Ksp22I	TGATCA	2 cut(s)	171, 901
Kzo9I	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
LmnI	GCTCC	8 cut(s)	181, 187, 193, 976, 1037, 1309, 1579, 1829
Lsp1109I	GCAGC	8 cut(s)	86, 350, 505, 667, 703, 1120, 1307, 1327
LweI	GCATC	8 cut(s)	637, 703, 766, 793, 842, 1151, 1701, 1765
MaeI	CTAG	3 cut(s)	1320, 1362, 1385
MaeII	ACGT	1 cut(s)	1764
MaeIII	GTNAC	6 cut(s)	28, 217, 304, 1038, 1182, 1372
MalI	GATC	9 cut(s)	68, 95, 127, 173, 567, 840, 903, 1149, 1164
MboI	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
MboII	GAAGA	8 cut(s)	217, 220, 290, 428, 956, 1279, 1456, 1622
MhlI	GDGCHC	5 cut(s)	198, 1061, 1384, 1489, 1645
MlsI	TGGCCA	1 cut(s)	1790
MluCI	AATT	6 cut(s)	365, 387, 860, 1256, 1365, 1447
MluNI	TGGCCA	1 cut(s)	1790
MlyI	GAGTC	3 cut(s)	388, 1234, 1293
MmeI	TCCRAC	2 cut(s)	36, 600
Mox20I	TGGCCA	1 cut(s)	1790
Mph1103I	ATGCAT	2 cut(s)	575, 1144
MroI	TCCGGA	1 cut(s)	973
MscI	TGGCCA	1 cut(s)	1790
MseI	TTAA	3 cut(s)	162, 1064, 1169
MslI	CAYNNNNRTG	2 cut(s)	909, 1821
Msp20I	TGGCCA	1 cut(s)	1790
MspA1I	CMGCKG	2 cut(s)	341, 1154
MspCI	CTTAAG	1 cut(s)	161
MspI	CCGG	5 cut(s)	185, 191, 243, 974, 1804
MspR9I	CCNGG	6 cut(s)	145, 1438, 1560, 1676, 1792, 1805
MvaI	CCWGG	5 cut(s)	145, 1438, 1560, 1676, 1792
MvnI	CGCG	2 cut(s)	54, 179
MwoI	GCNNNNNNNGC	8 cut(s)	163, 281, 475, 803, 1124, 1133, 1139, 1301
NciI	CCSGG	1 cut(s)	1805
NdeII	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
NlaIII	CATG	9 cut(s)	426, 597, 713, 767, 792, 908, 1252, 1373, 1789
NmeAIII	GCCGAG	1 cut(s)	49
NmuCI	GTSAC	4 cut(s)	28, 217, 304, 1182
NruI	TCGCGA	1 cut(s)	54
NsiI	ATGCAT	2 cut(s)	575, 1144
OliI	CACNNNNGTG	1 cut(s)	1821
PagI	TCATGA	1 cut(s)	709
PaqCI	CACCTGC	1 cut(s)	1195
PasI	CCCWGGG	1 cut(s)	144
PcsI	WCGNNNNNNNCGW	5 cut(s)	21, 51, 102, 561, 1343
PfeI	GAWTC	2 cut(s)	925, 1414
PfoI	TCCNGGA	1 cut(s)	1558
PinAI	ACCGGT	1 cut(s)	242
PkrI	GCNGC	9 cut(s)	101, 123, 340, 520, 682, 718, 1135, 1297, 1317
Ple19I	CGATCG	1 cut(s)	128
PleI	GAGTC	3 cut(s)	387, 1233, 1292
PpsI	GAGTC	3 cut(s)	387, 1233, 1292
PpuMI	RGGWCCY	2 cut(s)	1679, 1807
Psp124BI	GAGCTC	1 cut(s)	198
Psp5II	RGGWCCY	2 cut(s)	1679, 1807
Psp6I	CCWGG	5 cut(s)	143, 1436, 1558, 1674, 1790
PspEI	GGTNACC	1 cut(s)	28
PspFI	CCCAGC	1 cut(s)	1190
PspGI	CCWGG	5 cut(s)	143, 1436, 1558, 1674, 1790
PspPI	GGNCC	6 cut(s)	4, 550, 622, 1556, 1679, 1807
PspPPI	RGGWCCY	2 cut(s)	1679, 1807
PstI	CTGCAG	1 cut(s)	1596
PvuI	CGATCG	1 cut(s)	128
PvuII	CAGCTG	2 cut(s)	341, 1154
RruI	TCGCGA	1 cut(s)	54
RsaI	GTAC	4 cut(s)	247, 917, 1015, 1539
RsaNI	GTAC	4 cut(s)	246, 916, 1014, 1538
RseI	CAYNNNNRTG	2 cut(s)	909, 1821
SacI	GAGCTC	1 cut(s)	198
SaqAI	TTAA	3 cut(s)	162, 1064, 1169
SatI	GCNGC	9 cut(s)	100, 122, 339, 519, 681, 717, 1134, 1296, 1316
Sau3AI	GATC	9 cut(s)	66, 93, 125, 171, 565, 838, 901, 1147, 1162
Sau96I	GGNCC	6 cut(s)	4, 550, 622, 1556, 1679, 1807
SbfI	CCTGCAGG	1 cut(s)	1596
SchI	GAGTC	3 cut(s)	388, 1234, 1293
ScrFI	CCNGG	6 cut(s)	145, 1438, 1560, 1676, 1792, 1805
SdaI	CCTGCAGG	1 cut(s)	1596
SduI	GDGCHC	5 cut(s)	198, 1061, 1384, 1489, 1645
SfaNI	GCATC	8 cut(s)	637, 703, 766, 793, 842, 1151, 1701, 1765
SfcI	CTRYAG	1 cut(s)	1592
SinI	GGWCC	5 cut(s)	4, 622, 1556, 1679, 1807
SmiMI	CAYNNNNRTG	2 cut(s)	909, 1821
SmlI	CTYRAG	1 cut(s)	161
SmoI	CTYRAG	1 cut(s)	161
SpeI	ACTAGT	1 cut(s)	1384
Sse8387I	CCTGCAGG	1 cut(s)	1596
Sse9I	AATT	6 cut(s)	365, 387, 860, 1256, 1365, 1447
SsiI	CCGC	7 cut(s)	21, 121, 179, 1026, 1160, 1461, 1672
SspMI	CTAG	3 cut(s)	1320, 1362, 1385
SstI	GAGCTC	1 cut(s)	198
StyD4I	CCNGG	6 cut(s)	143, 1436, 1558, 1674, 1790, 1803
StyI	CCWWGG	3 cut(s)	264, 1307, 1686
TaaI	ACNGT	4 cut(s)	407, 920, 940, 1056
TaiI	ACGT	1 cut(s)	1767
TaqI	TCGA	9 cut(s)	45, 80, 128, 198, 634, 697, 858, 1081, 1165
TasI	AATT	6 cut(s)	365, 387, 860, 1256, 1365, 1447
TatI	WGTACW	1 cut(s)	915
TauI	GCSGC	1 cut(s)	124
TfiI	GAWTC	2 cut(s)	925, 1414
Tru1I	TTAA	3 cut(s)	162, 1064, 1169
Tru9I	TTAA	3 cut(s)	162, 1064, 1169
TscAI	CASTG	5 cut(s)	552, 595, 682, 883, 1003
TseFI	GTSAC	4 cut(s)	28, 217, 304, 1182
TseI	GCWGC	8 cut(s)	99, 338, 518, 680, 716, 1133, 1295, 1315
Tsp45I	GTSAC	4 cut(s)	28, 217, 304, 1182
TspDTI	ATGAA	8 cut(s)	312, 428, 698, 812, 1215, 1358, 1457, 1623
TspGWI	ACGGA	2 cut(s)	25, 459
TspRI	CASTG	5 cut(s)	552, 595, 682, 883, 1003
Vha464I	CTTAAG	1 cut(s)	161
VneI	GTGCAC	3 cut(s)	1057, 1380, 1641
VpaK11BI	GGWCC	5 cut(s)	4, 622, 1556, 1679, 1807
XagI	CCTNNNNNAGG	1 cut(s)	1241
XapI	RAATTY	4 cut(s)	860, 1256, 1365, 1447
XbaI	TCTAGA	1 cut(s)	1361
XspI	CTAG	3 cut(s)	1320, 1362, 1385
Zsp2I	ATGCAT	2 cut(s)	575, 1144

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.