Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

7987845 .. 7993443

Gene Structure 5599 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g08180.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGGAGTCGACTTCCGGCAGCTCGATGAAAATCTCGCCGTTGGATCTGATGGCGGCGATCTTCAGGGGCAAGATGGACCCCTCCAACACCTCCTCCGCCGGCGCCGAGGTTGCCTCCGTCATTCTCGAGAATCGGGAGTTCGTTATGATCCTGACGACCTCGATTGCCGTCTTGATCGGCTGCGTCGTCGTTTTTGTGTGGCGGCGGTCTAGTGCTCAAAAGCCTAAGCTTGTCCAGCCGCTTAAGCCGTTGGTTATTCCGGAGCCTGAGCCGGAGCTCGACGATGGAAGCAAGAAGGTCACTATCTTCTTCGGCACGCAAACCGGTACCGCTGAGGGGTTTGCCAAGGCTTTGGCTGAAGAGGCGAAAGCTCGCTACGATAAGGTCACATTCAAAGTTGTTGATTTGGACGATTATGCAGCTGATGATGAGGCATATGAGGAGAAATTGAAAAATGAGAGTTTGGCAATTTTCTTCTTGGCCACATATGGTGATGGTGAGCCAACGGATAATGCTGCTAGATTTTACAAGTGGTTTACAGAGGGTAAAGAGAAGGAAGCATGGCTTCAAAATCTTCATTACGGAGTGTTTGGTCTTGGAAACAGGCAGTATGAGCATTTCAATAAGGTGGCCGTGGTGGTGGATGAGATCCTTGCTGCCCAGGGTGGGAAGCGTCTTGTTCCAGTGGGCCTCGGAGATGACGATCAATGCATAGAGGATGATTTCACTGCATGGCGAGAATTGATGTGGCCTGAGTTGGACCAGTTGCTTCGAGATGAGGATGATGCAACCACACCTGCTTGCACACCTTACACTGCTGCTGTCTTGGAGTATCGAGTTGTATTTAATGATACCGCTGATGCATCAGTAGAGGATAAGAGCTGGAATAATGTAAATGGTCATGCTGTCATTGATGCTCAGCACCCATGCAGGGCTAATGTTGCCGTGAGAAAGGAACTTCATACCCCTGCATCTGATCGTTCTTGCACCCATCTCGAATTTGATATTGCTGGCACTGGACTTACATACGAAACTGGCGATCATGTTGGTGTGTACTGTGAGAATCTTTCTGAAACTGTGGAAGAGGCAATAAGTTTGTTGGGCCTGGCTCCGGAAGCACACTTTTCATTGTACACTGAGAAGGATGATGGTACACCACTAAGCGGAGGCTCCTTGTTGCCTCCCTTTCCACCATGCACATTAAGAACAGCACTTACTCGATATGCTGATCTTTTGAGTTCTCCTAAGAAGTCTGCCCTACTGGCTTTGGCTGCCTATGCATCTGATCCAGCTGAAGCGGATCGATTAAGACATCTTGCATCACCTGCTGGGAAGGATGAATATGCTCAATGGATGGTTGCAAGTCAGAGAAGCCTTCTTGAGGTCATGGCTGAATTTCCATCAGCCAAGCCCCCACTTGGAGTCTTCTTTGCAGCAGTTGCTCCGAGGTTGCAGCCTAGATACTACTCAATCTCTTCATCTCCAAGGATGGCACCATCTAGGATTCATGTCACTTGTGCACTAGTTTGTGACAAAACACCAACAGGAAGGATTCACAAAGGAATATGTTCAACCTGGATGAAGAATTCTGTGCCTTGGGCCAAAAGTGATGATTGTAGTTGGGCACCCATTTTTGTTCGGCAATCAAACTTCAGACTTCCTGTTGATAGTAAAGTACCTATTCTCATGATTGGTCCTGGAACTGGATTTGCTCCTTTCAGGGGTTTCCTGCAGGAAAGATTAGCTCTTAAAGAAGATGGAGCAGAACTTGGACCCTCCATTTTATTCTTTGGATGCAGAAATAGTCAAATGGACTATATTTATGAAGATGAGCTGAACAACTTTGTAGAAAAAGGTGCACTTTCTGAGCTTGTGGTTGCATTCTCCCGCCAGGGACCCACCAAGGAATATGTGCAACATAAAATGATGCAGAAGGCTTCGGATATCTGGAACATGATATCTCAGGGAGGTTATATATACGTTTGTGGTGATGCTAAGGGCATGGCCAAGGATGTCCACCGGACTCTTCACACAATCTTGCAGGAGCAGGGATCTCTGGACAGCTCGAAAGCTGAGAGCATGGTGAAAAATCTGCAAACGAATGGCAGGTATCTGCGTGATGTGTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

710

Amino Acids

78.31

Weight (kDa)

5.14

Isoelectric Point (pI)

40.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	102 - 245	5.3e-33	Flavodoxin
FAD_binding_1	PF00667	305 - 526	8e-70	FAD binding domain
NAD_binding_1	PF00175	563 - 673	3e-16	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	805, 1333
Acc36I	ACCTGC	3 cut(s)	805, 1333, 2097
Acc65I	GGTACC	1 cut(s)	326
AccB1I	GGYRCC	4 cut(s)	101, 326, 1492, 1624
AccI	GTMKAC	1 cut(s)	8
AccIII	TCCGGA	2 cut(s)	259, 1111
AclWI	GGATC	6 cut(s)	51, 142, 643, 1280, 1308, 2059
AcoI	YGGCCR	3 cut(s)	480, 630, 2004
AcsI	RAATTY	3 cut(s)	998, 1394, 1585
AcuI	CTGAAG	4 cut(s)	46, 378, 1314, 1636
AcyI	GRCGYC	1 cut(s)	102
AfaI	GTAC	5 cut(s)	328, 1055, 1133, 1153, 1677
AfiI	CCNNNNNNNGG	9 cut(s)	336, 666, 931, 1111, 1163, 1381, 1418, 1703, 1721
AflII	CTTAAG	1 cut(s)	242
AgeI	ACCGGT	1 cut(s)	323
AgsI	TTSAA	5 cut(s)	394, 451, 569, 622, 1572
AhlI	ACTAGT	1 cut(s)	1522
AjnI	CCWGG	5 cut(s)	660, 1104, 1574, 1696, 1890
Alw21I	GWGCWC	4 cut(s)	217, 279, 1522, 1861
Alw44I	GTGCAC	2 cut(s)	1518, 1857
AlwI	GGATC	6 cut(s)	51, 142, 643, 1280, 1308, 2059
Ama87I	CYCGRG	1 cut(s)	125
Aor13HI	TCCGGA	2 cut(s)	259, 1111
AoxI	GGCC	7 cut(s)	480, 630, 688, 749, 1102, 1599, 2004
ApaLI	GTGCAC	2 cut(s)	1518, 1857
ApeKI	GCWGC	9 cut(s)	18, 180, 419, 515, 656, 818, 1271, 1433, 1453
ApoI	RAATTY	3 cut(s)	998, 1394, 1585
AsiGI	ACCGGT	1 cut(s)	323
Asp700I	GAANNNNTTC	1 cut(s)	1567
Asp718I	GGTACC	1 cut(s)	326
AspLEI	GCGC	1 cut(s)	104
AspS9I	GGNCC	8 cut(s)	76, 688, 760, 1102, 1599, 1694, 1772, 1895
AsuHPI	GGTGA	5 cut(s)	503, 509, 1314, 2000, 2095
AvaI	CYCGRG	1 cut(s)	125
AvaII	GGWCC	5 cut(s)	76, 760, 1694, 1772, 1895
BaeGI	GKGCMC	3 cut(s)	1522, 1627, 1861
BalI	TGGCCA	2 cut(s)	482, 2006
BanI	GGYRCC	4 cut(s)	101, 326, 1492, 1624
BanII	GRGCYC	1 cut(s)	279
BbsI	GAAGAC	1 cut(s)	1417
Bbv12I	GWGCWC	4 cut(s)	217, 279, 1522, 1861
BbvCI	CCTCAGC	1 cut(s)	333
BbvI	GCAGC	9 cut(s)	30, 167, 431, 502, 643, 805, 1258, 1445, 1465
BceAI	ACGGC	5 cut(s)	22, 152, 232, 617, 929
BcgI	CGANNNNNNTGC	2 cut(s)	976, 1010
BciT130I	CCWGG	5 cut(s)	662, 1106, 1576, 1698, 1892
BcuI	ACTAGT	1 cut(s)	1522
BfaI	CTAG	5 cut(s)	210, 519, 1458, 1500, 1523
BfmI	CTRYAG	1 cut(s)	1730
BfoI	RGCGCY	1 cut(s)	105
BfrI	CTTAAG	1 cut(s)	242
BfuAI	ACCTGC	3 cut(s)	805, 1333, 2097
BglI	GCCNNNNNGGC	1 cut(s)	1262
BlpI	GCTNAGC	1 cut(s)	918
Bme1390I	CCNGG	5 cut(s)	662, 1106, 1576, 1698, 1892
Bme18I	GGWCC	5 cut(s)	76, 760, 1694, 1772, 1895
BmeT110I	CYCGRG	1 cut(s)	125
BmgT120I	GGNCC	8 cut(s)	76, 688, 760, 1102, 1599, 1694, 1772, 1895
BmrFI	CCNGG	5 cut(s)	662, 1106, 1576, 1698, 1892
BpiI	GAAGAC	1 cut(s)	1417
BplI	GAGNNNNNCTC	2 cut(s)	98, 130
Bpu10I	CCTNAGC	4 cut(s)	225, 267, 333, 1995
Bpu1102I	GCTNAGC	1 cut(s)	918
BpuEI	CTTGAG	1 cut(s)	1400
Bsa29I	ATCGAT	1 cut(s)	1303
BsaBI	GATNNNNATC	2 cut(s)	29, 702
BsaHI	GRCGYC	1 cut(s)	102
BsaWI	WCCGGW	4 cut(s)	259, 323, 1111, 2019
BsaXI	ACNNNNNCTCC	2 cut(s)	77, 107
Bsc4I	CCNNNNNNNGG	9 cut(s)	336, 666, 931, 1111, 1163, 1381, 1418, 1703, 1721
Bse118I	RCCGGY	2 cut(s)	98, 323
Bse1I	ACTGG	6 cut(s)	683, 763, 1021, 1039, 1266, 1708
Bse8I	GATNNNNATC	2 cut(s)	29, 702
BseAI	TCCGGA	2 cut(s)	259, 1111
BseBI	CCWGG	5 cut(s)	662, 1106, 1576, 1698, 1892
BseCI	ATCGAT	1 cut(s)	1303
BseJI	GATNNNNATC	2 cut(s)	29, 702
BseLI	CCNNNNNNNGG	9 cut(s)	336, 666, 931, 1111, 1163, 1381, 1418, 1703, 1721
BseMII	CTCAG	8 cut(s)	258, 324, 744, 932, 1128, 1857, 1976, 2064
BseNI	ACTGG	6 cut(s)	683, 763, 1021, 1039, 1266, 1708
BseRI	GAGGAG	2 cut(s)	82, 455
BseSI	GKGCMC	3 cut(s)	1522, 1627, 1861
BseXI	GCAGC	9 cut(s)	30, 167, 431, 502, 643, 805, 1258, 1445, 1465
BseYI	CCCAGC	1 cut(s)	1328
BshFI	GGCC	7 cut(s)	482, 632, 690, 751, 1104, 1601, 2006
BshNI	GGYRCC	4 cut(s)	101, 326, 1492, 1624
BshTI	ACCGGT	1 cut(s)	323
BshVI	ATCGAT	1 cut(s)	1303
BsiHKAI	GWGCWC	4 cut(s)	217, 279, 1522, 1861
BsiHKCI	CYCGRG	1 cut(s)	125
BsiSI	CCGG	7 cut(s)	15, 99, 260, 272, 324, 1112, 2020
BslFI	GGGAC	1 cut(s)	1908
BslI	CCNNNNNNNGG	9 cut(s)	336, 666, 931, 1111, 1163, 1381, 1418, 1703, 1721
BsmFI	GGGAC	1 cut(s)	1908
BsmI	GAATGC	1 cut(s)	1880
BsnI	GGCC	7 cut(s)	482, 632, 690, 751, 1104, 1601, 2006
BsoBI	CYCGRG	1 cut(s)	125
Bsp1286I	GDGCHC	5 cut(s)	217, 279, 1522, 1627, 1861
Bsp13I	TCCGGA	2 cut(s)	259, 1111
Bsp1407I	TGTACA	1 cut(s)	1131
Bsp1720I	GCTNAGC	1 cut(s)	918
BspANI	GGCC	7 cut(s)	482, 632, 690, 751, 1104, 1601, 2006
BspCNI	CTCAG	8 cut(s)	259, 325, 745, 931, 1129, 1858, 1975, 2065
BspDI	ATCGAT	1 cut(s)	1303
BspEI	TCCGGA	2 cut(s)	259, 1111
BspHI	TCATGA	1 cut(s)	1686
BspMAI	CTGCAG	1 cut(s)	1734
BspMI	ACCTGC	3 cut(s)	805, 1333, 2097
BspPI	GGATC	6 cut(s)	51, 142, 643, 1280, 1308, 2059
BspT107I	GGYRCC	4 cut(s)	101, 326, 1492, 1624
BspTI	CTTAAG	1 cut(s)	242
BsrFI	RCCGGY	2 cut(s)	98, 323
BsrGI	TGTACA	1 cut(s)	1131
BsrI	ACTGG	6 cut(s)	683, 763, 1021, 1039, 1266, 1708
BssAI	RCCGGY	2 cut(s)	98, 323
BssNI	GRCGYC	1 cut(s)	102
BssT1I	CCWWGG	5 cut(s)	345, 1484, 1595, 1902, 2007
Bst2UI	CCWGG	5 cut(s)	662, 1106, 1576, 1698, 1892
Bst4CI	ACNGT	2 cut(s)	1058, 1078
Bst6I	CTCTTC	4 cut(s)	354, 1077, 1480, 2031
BstACI	GRCGYC	1 cut(s)	102
BstAFI	CTTAAG	1 cut(s)	242
BstAPI	GCANNNNNTGC	2 cut(s)	1325, 1439
BstAUI	TGTACA	1 cut(s)	1131
BstC8I	GCNNGC	5 cut(s)	100, 317, 373, 802, 1012
BstDSI	CCRYGG	1 cut(s)	633
BstENI	CCTNNNNNAGG	1 cut(s)	1379
BstH2I	RGCGCY	1 cut(s)	105
BstHHI	GCGC	1 cut(s)	104
BstNI	CCWGG	5 cut(s)	662, 1106, 1576, 1698, 1892
BstSCI	CCNGG	5 cut(s)	660, 1104, 1574, 1696, 1890
BstSFI	CTRYAG	1 cut(s)	1730
BstSLI	GKGCMC	3 cut(s)	1522, 1627, 1861
BstV1I	GCAGC	9 cut(s)	30, 167, 431, 502, 643, 805, 1258, 1445, 1465
BstV2I	GAAGAC	1 cut(s)	1417
BstX2I	RGATCY	3 cut(s)	43, 648, 2051
BstXI	CCANNNNNNTGG	1 cut(s)	352
BstYI	RGATCY	3 cut(s)	43, 648, 2051
Bsu15I	ATCGAT	1 cut(s)	1303
BsuRI	GGCC	7 cut(s)	482, 632, 690, 751, 1104, 1601, 2006
BsuTUI	ATCGAT	1 cut(s)	1303
BtgI	CCRYGG	1 cut(s)	633
BtsI	GCAGTG	2 cut(s)	726, 813
BtsIMutI	CAGTG	5 cut(s)	690, 726, 813, 1014, 1134
BveI	ACCTGC	3 cut(s)	805, 1333, 2097
Cac8I	GCNNGC	5 cut(s)	100, 317, 373, 802, 1012
CciI	TCATGA	1 cut(s)	1686
CfoI	GCGC	1 cut(s)	104
Cfr10I	RCCGGY	2 cut(s)	98, 323
Cfr13I	GGNCC	8 cut(s)	76, 688, 760, 1102, 1599, 1694, 1772, 1895
ClaI	ATCGAT	1 cut(s)	1303
CseI	GACGC	2 cut(s)	172, 662
Csp6I	GTAC	5 cut(s)	327, 1054, 1132, 1152, 1676
CspAI	ACCGGT	1 cut(s)	323
CviQI	GTAC	5 cut(s)	327, 1054, 1132, 1152, 1676
DinI	GGCGCC	1 cut(s)	103
EaeI	YGGCCR	3 cut(s)	480, 630, 2004
Eam1104I	CTCTTC	4 cut(s)	354, 1077, 1480, 2031
EarI	CTCTTC	4 cut(s)	354, 1077, 1480, 2031
EciI	GGCGGA	1 cut(s)	85
Ecl136II	GAGCTC	1 cut(s)	277
Eco130I	CCWWGG	5 cut(s)	345, 1484, 1595, 1902, 2007
Eco24I	GRGCYC	1 cut(s)	279
Eco32I	GATATC	2 cut(s)	1945, 1959
Eco47I	GGWCC	5 cut(s)	76, 760, 1694, 1772, 1895
Eco53kI	GAGCTC	1 cut(s)	277
Eco57I	CTGAAG	4 cut(s)	46, 378, 1314, 1636
Eco88I	CYCGRG	1 cut(s)	125
EcoICRI	GAGCTC	1 cut(s)	277
EcoNI	CCTNNNNNAGG	1 cut(s)	1379
EcoO109I	RGGNCCY	1 cut(s)	1895
EcoRI	GAATTC	1 cut(s)	1585
EcoRII	CCWGG	5 cut(s)	660, 1104, 1574, 1696, 1890
EcoRV	GATATC	2 cut(s)	1945, 1959
EcoT14I	CCWWGG	5 cut(s)	345, 1484, 1595, 1902, 2007
EcoT22I	ATGCAT	3 cut(s)	713, 865, 1282
EcoT38I	GRGCYC	1 cut(s)	279
EgeI	GGCGCC	1 cut(s)	103
EheI	GGCGCC	1 cut(s)	103
ErhI	CCWWGG	5 cut(s)	345, 1484, 1595, 1902, 2007
FalI	AAGNNNNNCTT	4 cut(s)	549, 581, 1845, 1877
FaqI	GGGAC	1 cut(s)	1908
FauI	CCCGC	1 cut(s)	1895
FauNDI	CATATG	2 cut(s)	436, 487
FblI	GTMKAC	1 cut(s)	8
FriOI	GRGCYC	1 cut(s)	279
FspBI	CTAG	5 cut(s)	210, 519, 1458, 1500, 1523
GlaI	GCGC	1 cut(s)	103
GsaI	CCCAGC	1 cut(s)	1332
HaeII	RGCGCY	1 cut(s)	105
HaeIII	GGCC	7 cut(s)	482, 632, 690, 751, 1104, 1601, 2006
HapII	CCGG	7 cut(s)	15, 99, 260, 272, 324, 1112, 2020
HgaI	GACGC	2 cut(s)	172, 662
HhaI	GCGC	1 cut(s)	104
Hin1I	GRCGYC	1 cut(s)	102
Hin6I	GCGC	1 cut(s)	102
HinP1I	GCGC	1 cut(s)	102
HincII	GTYRAC	1 cut(s)	9
HindII	GTYRAC	1 cut(s)	9
HindIII	AAGCTT	1 cut(s)	227
HinfI	GANTC	7 cut(s)	5, 130, 1063, 1422, 1504, 1552, 2023
HpaII	CCGG	7 cut(s)	15, 99, 260, 272, 324, 1112, 2020
HphI	GGTGA	5 cut(s)	503, 509, 1314, 2000, 2095
Hpy166II	GTNNAC	8 cut(s)	9, 537, 1054, 1134, 1154, 1520, 1859, 2017
Hpy8I	GTNNAC	8 cut(s)	9, 537, 1054, 1134, 1154, 1520, 1859, 2017
Hpy99I	CGWCG	3 cut(s)	188, 191, 284
HpyAV	CCTTC	7 cut(s)	289, 547, 1135, 1327, 1385, 1542, 1927
HpyCH4III	ACNGT	2 cut(s)	1058, 1078
HpyCH4IV	ACGT	1 cut(s)	1980
HpySE526I	ACGT	1 cut(s)	1980
Hsp92I	GRCGYC	1 cut(s)	102
HspAI	GCGC	1 cut(s)	102
KasI	GGCGCC	1 cut(s)	101
KflI	GGGWCCC	1 cut(s)	1895
Kpn2I	TCCGGA	2 cut(s)	259, 1111
KpnI	GGTACC	1 cut(s)	330
KroI	GCCGGC	1 cut(s)	98
KroNI	GCCGGC	1 cut(s)	100
LmnI	GCTCC	8 cut(s)	262, 274, 1114, 1175, 1447, 1717, 1760, 2044
Lsp1109I	GCAGC	9 cut(s)	30, 167, 431, 502, 643, 805, 1258, 1445, 1465
MaeI	CTAG	5 cut(s)	210, 519, 1458, 1500, 1523
MaeII	ACGT	1 cut(s)	1980
MaeIII	GTNAC	4 cut(s)	298, 385, 1510, 1529
MflI	RGATCY	3 cut(s)	43, 648, 2051
MhlI	GDGCHC	5 cut(s)	217, 279, 1522, 1627, 1861
MlsI	TGGCCA	2 cut(s)	482, 2006
MluCI	AATT	6 cut(s)	446, 468, 740, 998, 1394, 1585
MluNI	TGGCCA	2 cut(s)	482, 2006
Mly113I	GGCGCC	1 cut(s)	102
MlyI	GAGTC	3 cut(s)	14, 1431, 2017
MmeI	TCCRAC	3 cut(s)	21, 108, 738
Mox20I	TGGCCA	2 cut(s)	482, 2006
Mph1103I	ATGCAT	3 cut(s)	713, 865, 1282
MreI	CGCCGGCG	1 cut(s)	98
MroI	TCCGGA	2 cut(s)	259, 1111
MroNI	GCCGGC	1 cut(s)	98
MroXI	GAANNNNTTC	1 cut(s)	1567
MscI	TGGCCA	2 cut(s)	482, 2006
MseI	TTAA	5 cut(s)	243, 846, 1202, 1307, 1749
MslI	CAYNNNNRTG	1 cut(s)	1047
Msp20I	TGGCCA	2 cut(s)	482, 2006
MspA1I	CMGCKG	4 cut(s)	332, 422, 857, 1292
MspCI	CTTAAG	1 cut(s)	242
MspI	CCGG	7 cut(s)	15, 99, 260, 272, 324, 1112, 2020
MspR9I	CCNGG	5 cut(s)	662, 1106, 1576, 1698, 1892
Mva1269I	GAATGC	1 cut(s)	1880
MvaI	CCWGG	5 cut(s)	662, 1106, 1576, 1698, 1892
NaeI	GCCGGC	1 cut(s)	100
NarI	GGCGCC	1 cut(s)	102
NdeI	CATATG	2 cut(s)	436, 487
NgoMIV	GCCGGC	1 cut(s)	98
NmeAIII	GCCGAG	1 cut(s)	130
NmuCI	GTSAC	4 cut(s)	298, 385, 1510, 1529
NsiI	ATGCAT	3 cut(s)	713, 865, 1282
PaeR7I	CTCGAG	1 cut(s)	125
PagI	TCATGA	1 cut(s)	1686
PaqCI	CACCTGC	2 cut(s)	805, 1333
PasI	CCCWGGG	1 cut(s)	661
PcsI	WCGNNNNNNNCGW	3 cut(s)	183, 699, 1035
PctI	GAATGC	1 cut(s)	1880
PdiI	GCCGGC	1 cut(s)	100
PdmI	GAANNNNTTC	1 cut(s)	1567
PfeI	GAWTC	4 cut(s)	130, 1063, 1504, 1552
PfoI	TCCNGGA	1 cut(s)	1696
PinAI	ACCGGT	1 cut(s)	323
PleI	GAGTC	3 cut(s)	13, 1430, 2017
PluTI	GGCGCC	1 cut(s)	105
PpsI	GAGTC	3 cut(s)	13, 1430, 2017
PpuMI	RGGWCCY	1 cut(s)	1895
Psp124BI	GAGCTC	1 cut(s)	279
Psp5II	RGGWCCY	1 cut(s)	1895
Psp6I	CCWGG	5 cut(s)	660, 1104, 1574, 1696, 1890
PspFI	CCCAGC	1 cut(s)	1328
PspGI	CCWGG	5 cut(s)	660, 1104, 1574, 1696, 1890
PspPI	GGNCC	8 cut(s)	76, 688, 760, 1102, 1599, 1694, 1772, 1895
PspPPI	RGGWCCY	1 cut(s)	1895
PsrI	GAACNNNNNNTAC	2 cut(s)	1198, 1230
PstI	CTGCAG	1 cut(s)	1734
PsuI	RGATCY	3 cut(s)	43, 648, 2051
PvuII	CAGCTG	2 cut(s)	422, 1292
RsaI	GTAC	5 cut(s)	328, 1055, 1133, 1153, 1677
RsaNI	GTAC	5 cut(s)	327, 1054, 1132, 1152, 1676
RseI	CAYNNNNRTG	1 cut(s)	1047
SacI	GAGCTC	1 cut(s)	279
SalI	GTCGAC	1 cut(s)	7
SaqAI	TTAA	5 cut(s)	243, 846, 1202, 1307, 1749
Sau96I	GGNCC	3 cut(s)	76, 688, 760
SbfI	CCTGCAGG	1 cut(s)	1734
SchI	GAGTC	3 cut(s)	14, 1431, 2017
ScrFI	CCNGG	5 cut(s)	662, 1106, 1576, 1698, 1892
SdaI	CCTGCAGG	1 cut(s)	1734
SduI	GDGCHC	5 cut(s)	217, 279, 1522, 1627, 1861
SfcI	CTRYAG	1 cut(s)	1730
SfoI	GGCGCC	1 cut(s)	103
Sfr274I	CTCGAG	1 cut(s)	125
SgrAI	CRCCGGYG	1 cut(s)	98
SinI	GGWCC	5 cut(s)	76, 760, 1694, 1772, 1895
SlaI	CTCGAG	1 cut(s)	125
SmiMI	CAYNNNNRTG	1 cut(s)	1047
SmlI	CTYRAG	3 cut(s)	125, 242, 1379
SmoI	CTYRAG	3 cut(s)	125, 242, 1379
SpeI	ACTAGT	1 cut(s)	1522
Sse8387I	CCTGCAGG	1 cut(s)	1734
Sse9I	AATT	6 cut(s)	446, 468, 740, 998, 1394, 1585
SspDI	GGCGCC	1 cut(s)	101
SspMI	CTAG	5 cut(s)	210, 519, 1458, 1500, 1523
SstI	GAGCTC	1 cut(s)	279
StyD4I	CCNGG	5 cut(s)	660, 1104, 1574, 1696, 1890
StyI	CCWWGG	5 cut(s)	345, 1484, 1595, 1902, 2007
TaaI	ACNGT	2 cut(s)	1058, 1078
TaiI	ACGT	1 cut(s)	1983
TasI	AATT	6 cut(s)	446, 468, 740, 998, 1394, 1585
TatI	WGTACW	2 cut(s)	1053, 1131
TauI	GCSGC	3 cut(s)	56, 205, 241
TfiI	GAWTC	4 cut(s)	130, 1063, 1504, 1552
Tru1I	TTAA	5 cut(s)	243, 846, 1202, 1307, 1749
Tru9I	TTAA	5 cut(s)	243, 846, 1202, 1307, 1749
TscAI	CASTG	5 cut(s)	690, 733, 820, 1021, 1141
TseFI	GTSAC	4 cut(s)	298, 385, 1510, 1529
TseI	GCWGC	9 cut(s)	18, 180, 419, 515, 656, 818, 1271, 1433, 1453
Tsp45I	GTSAC	4 cut(s)	298, 385, 1510, 1529
TspDTI	ATGAA	9 cut(s)	41, 566, 950, 1116, 1353, 1467, 1496, 1595, 1839
TspGWI	ACGGA	3 cut(s)	106, 521, 597
TspRI	CASTG	5 cut(s)	690, 733, 820, 1021, 1141
Vha464I	CTTAAG	1 cut(s)	242
VneI	GTGCAC	2 cut(s)	1518, 1857
VpaK11BI	GGWCC	5 cut(s)	76, 760, 1694, 1772, 1895
XagI	CCTNNNNNAGG	1 cut(s)	1379
XapI	RAATTY	3 cut(s)	998, 1394, 1585
XhoI	CTCGAG	1 cut(s)	125
XmiI	GTMKAC	1 cut(s)	8
XmnI	GAANNNNTTC	1 cut(s)	1567
XspI	CTAG	5 cut(s)	210, 519, 1458, 1500, 1523
Zsp2I	ATGCAT	3 cut(s)	713, 865, 1282

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.