Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

30216637 .. 30221654

Gene Structure 5018 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1257600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGGAGTCCTCTTCTAACTCGATGAAAATCTCGCCGTTGGATCTGATGGCGGCGATTATCAAGGGCAAGATGGACCCCGCCGACTTCTCCGGTGCCCAAGTCACCTCAGTCATTCTCGAGAACCGCGAGTTCGTCATGATCTTGACCACCTCCATTGCTGTCCTCATCGGCTGCGTCGTCGTTTTGGTCTTGCGGCGATCCAGTGCCCAGAAGCACCCCGGCGTCATCCAGTCGCCTAGGCCCCTGATCGTGGAGGAGCCGGAGCCGGTGGAGGCTGACAACGGCAGCAAGAAGGTCACCATCTTCTTCGGCACTCAGACTGGCACCGCCGAAGGTTTCGCCAAGGCTTTGGCCGAGGAAGCAAAAGCTCGATACGAGAAGGTCACCTTCAAAGTTGTTGATTTGGACGATTACGCTGCCGATGACGAAGCATATGAGGAGAAGTTGAAGAATGAGAGCTTGGCATTTTTCTTCCTGGCCACATATGGTGATGGTGAGCCAACCGACAATGCTGCGAGGTTTTACAAGTGGTTTACAGAGGGTGAAGAAAGAGGGGAGTGGCTTAAGAATCTTAACTACGGGGTGTTTGGCCTTGGAAACAGACAGTATGAGCATTTCAATAAGGTTGCCGTGGTTGTGGATGAGATCCTCGCTGCACAGGGTGGGAAGCGACTTGTTAAAGTGGGTCTTGGAGATGATGATCAGTGCATTGAGGATGACTTCTCTGCATGGCGAGAGTTGCTGTGGCCTGAGTTGGATCAGTTGCTTCGAGATGAGGATGATGTAATGTCACCTGTTTCTACACCTTACACTGCTGCTGTGTTGGAGTATCGAGTTGTAATTAAAGATGCTGCAGATGCATCAGTAGATAGTAAGAGCTGGAATAATGCAAATGGTCATGCTGTCATCGATGCCCAACATCCATGCAGAGCTAATGTGGCTGTGAGAAGGGAACTTCATACTCCTGCATCTGATCGTTCTTGCACCCATCTTGAGTTTGATATTTCTGGCACTGGACTTACATACGAAACTGGGGATCATGTTGGTGTTTACTGTGAGAATCTTCCTGAAATTGTGGAAGAGGCACTAAGTTTGTTGGGCTTGTCCCCAGAAATATACTTCTCGATACACACTGATAAAGAGGATGGCACACCACTAAGTGGAAGCTCCTTGACACCTCCCTTCCCACCATGCACATTAAAAACAGCACTAACTCGATACGCTGATGTTTTAACTTCTCCGAAAAAGTCTGCCTTGCTCGCTTTAGCAGCACATGCATCTGACCCAACCGAAGCTGATCGATTAAGACATCTTGCATCACCAGCTGGGAAGGTGGATGAATATGCACAATTGGTGGTTGCAAGTCAGAGAAGCCTCCTTGAGGTCATGGCTGAATTTCCATCGGCCAAGCCTCCACTTGGTGTCTTTTTTGCAGCAGTTGCACCAAGATTGCAGCCTAGATACTATTCAATCTCGTCATCCTCAAGGATGGCATCTTCTAGAATTCACGTTACTTGTGCATTAGTATATGAGAAAACACCAGCAGGGAGGATACACAAAGGAGTGTGTTCAACCTGGATGAAGAATTCCGTTCCTTTGGAGAAGAGTGATGACTGTAGCTGGGCTCCAATTTTTGTTCGGCAGTCCAACTTCAAACTTCCTGCAGATACTAAAGTACCTATTATCATGATTGGTCCTGGAACCGGATTTGCTCCATTCAGGGGTTTCCTGCAGGAAAGATTGGCTCTTAAAGAAGATGGAGCAGAACTAGGACCCTCCACATTATTCTTTGGATGTAGAAATAGTAAAATGGATTACATTTATGAAGATGAGCTTAACAACTTTTTAGAAACCAGTGCACTGTCTGAGCTGGTGGTTGCATTCTCACGTCAGGGACCTACCAAGGAATATGTGCAGCACAAAATGATTCAGAAGGCCTCAGACGTCTGGAACATGATATCTCAGGGTGGTTATATTTACGTCTGTGGTGATGCTAAGGGCATGGCCAGGGATGTCCACCGGACCCTTCACACAATCGTGCAGGAGCAGGGATGTATGGGCAGCTCCAAAGCAGAGAGCCTGGTGAAAAATCTGCAAATGAACGGAAGGTATCTGCGCGACGTTTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

710

Amino Acids

78.17

Weight (kDa)

5.22

Isoelectric Point (pI)

38.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	101 - 244	3.1e-32	Flavodoxin
FAD_binding_1	PF00667	304 - 526	5e-68	FAD binding domain
NAD_binding_1	PF00175	563 - 673	8.5e-16	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1947
AccB1I	GGYRCC	2 cut(s)	92, 323
AccB7I	CCANNNNNTGG	1 cut(s)	1160
AccII	CGCG	2 cut(s)	126, 2118
AciI	CCGC	5 cut(s)	50, 78, 124, 193, 327
AclWI	GGATC	5 cut(s)	48, 192, 640, 765, 1044
AcoI	YGGCCR	4 cut(s)	351, 477, 1404, 2004
AcsI	RAATTY	3 cut(s)	1394, 1503, 1585
AcyI	GRCGYC	2 cut(s)	222, 1944
AdeI	CACNNNGTG	3 cut(s)	662, 1160, 1421
AfaI	GTAC	1 cut(s)	1677
AfiI	CCNNNNNNNGG	6 cut(s)	250, 1160, 1381, 1418, 1703, 1721
AflII	CTTAAG	1 cut(s)	563
AgsI	TTSAA	6 cut(s)	391, 448, 619, 1470, 1572, 1654
AjiI	CACGTC	1 cut(s)	1889
AjnI	CCWGG	5 cut(s)	474, 1574, 1696, 2006, 2079
AjuI	GAANNNNNNNTTGG	2 cut(s)	443, 475
AleI	CACNNNNGTG	1 cut(s)	2036
Alw21I	GWGCWC	1 cut(s)	1861
Alw44I	GTGCAC	1 cut(s)	1857
AlwI	GGATC	5 cut(s)	48, 192, 640, 765, 1044
Ama87I	CYCGRG	1 cut(s)	116
AoxI	GGCC	8 cut(s)	239, 351, 477, 589, 746, 1404, 1935, 2004
ApaLI	GTGCAC	1 cut(s)	1857
ApoI	RAATTY	3 cut(s)	1394, 1503, 1585
AspA2I	CCTAGG	1 cut(s)	236
AspLEI	GCGC	1 cut(s)	2118
AspS9I	GGNCC	6 cut(s)	73, 240, 1694, 1772, 1895, 2022
AsuC2I	CCSGG	1 cut(s)	219
AvaI	CYCGRG	1 cut(s)	116
AvaII	GGWCC	5 cut(s)	73, 1694, 1772, 1895, 2022
AvrII	CCTAGG	1 cut(s)	236
BaeGI	GKGCMC	3 cut(s)	97, 208, 1861
BaeI	ACNNNNGTAYC	4 cut(s)	1659, 1659, 1692, 1692
BalI	TGGCCA	2 cut(s)	479, 2006
BanI	GGYRCC	2 cut(s)	92, 323
BanII	GRGCYC	1 cut(s)	1627
Bbv12I	GWGCWC	1 cut(s)	1861
BccI	CCATC	9 cut(s)	40, 64, 308, 485, 996, 1139, 1408, 1483, 1751
BceAI	ACGGC	3 cut(s)	19, 298, 614
BcgI	CGANNNNNNTGC	4 cut(s)	398, 432, 494, 528
BciT130I	CCWGG	5 cut(s)	476, 1576, 1698, 2008, 2081
BciVI	GTATCC	1 cut(s)	1545
BclI	TGATCA	1 cut(s)	700
BcnI	CCSGG	1 cut(s)	219
BfaI	CTAG	4 cut(s)	237, 1458, 1500, 1769
BfmI	CTRYAG	4 cut(s)	852, 1615, 1662, 1730
BfrI	CTTAAG	1 cut(s)	563
BfuI	GTATCC	1 cut(s)	1545
BlnI	CCTAGG	1 cut(s)	236
Bme1390I	CCNGG	6 cut(s)	219, 476, 1576, 1698, 2008, 2081
Bme18I	GGWCC	5 cut(s)	73, 1694, 1772, 1895, 2022
BmeT110I	CYCGRG	1 cut(s)	116
BmgBI	CACGTC	1 cut(s)	1889
BmgT120I	GGNCC	6 cut(s)	73, 240, 1694, 1772, 1895, 2022
BmrFI	CCNGG	6 cut(s)	219, 476, 1576, 1698, 2008, 2081
BmrI	ACTGGG	1 cut(s)	1041
BmsI	GCATC	9 cut(s)	838, 847, 869, 901, 977, 1286, 1325, 1502, 1981
BmuI	ACTGGG	1 cut(s)	1041
Bpu10I	CCTNAGC	1 cut(s)	1995
BpuEI	CTTGAG	3 cut(s)	1013, 1400, 1468
BpuMI	CCSGG	1 cut(s)	219
Bsa29I	ATCGAT	2 cut(s)	909, 1300
BsaBI	GATNNNNATC	2 cut(s)	26, 699
BsaHI	GRCGYC	2 cut(s)	222, 1944
BsaJI	CCNNGG	8 cut(s)	217, 236, 342, 354, 592, 630, 1902, 2007
BsaWI	WCCGGW	3 cut(s)	89, 1703, 2019
Bsc4I	CCNNNNNNNGG	6 cut(s)	250, 1160, 1381, 1418, 1703, 1721
Bse118I	RCCGGY	1 cut(s)	265
Bse1I	ACTGG	6 cut(s)	201, 229, 325, 1018, 1036, 1854
Bse3DI	GCAATG	1 cut(s)	153
Bse8I	GATNNNNATC	2 cut(s)	26, 699
BseBI	CCWGG	5 cut(s)	476, 1576, 1698, 2008, 2081
BseCI	ATCGAT	2 cut(s)	909, 1300
BseDI	CCNNGG	8 cut(s)	217, 236, 342, 354, 592, 630, 1902, 2007
BseJI	GATNNNNATC	2 cut(s)	26, 699
BseLI	CCNNNNNNNGG	6 cut(s)	250, 1160, 1381, 1418, 1703, 1721
BseMI	GCAATG	1 cut(s)	153
BseMII	CTCAG	6 cut(s)	120, 329, 741, 1857, 1953, 1976
BseNI	ACTGG	6 cut(s)	201, 229, 325, 1018, 1036, 1854
BseRI	GAGGAG	2 cut(s)	269, 452
BseSI	GKGCMC	3 cut(s)	97, 208, 1861
BseYI	CCCAGC	2 cut(s)	1325, 1620
BsgI	GTGCAG	3 cut(s)	639, 1934, 2060
Bsh1236I	CGCG	2 cut(s)	126, 2118
BshFI	GGCC	8 cut(s)	241, 353, 479, 591, 748, 1406, 1937, 2006
BshNI	GGYRCC	2 cut(s)	92, 323
BshVI	ATCGAT	2 cut(s)	909, 1300
BsiHKAI	GWGCWC	1 cut(s)	1861
BsiHKCI	CYCGRG	1 cut(s)	116
BsiSI	CCGG	6 cut(s)	90, 219, 260, 266, 1704, 2020
BslFI	GGGAC	2 cut(s)	1090, 1908
BslI	CCNNNNNNNGG	6 cut(s)	250, 1160, 1381, 1418, 1703, 1721
BsmFI	GGGAC	2 cut(s)	1090, 1908
BsmI	GAATGC	1 cut(s)	1880
BsnI	GGCC	8 cut(s)	241, 353, 479, 591, 748, 1406, 1937, 2006
BsoBI	CYCGRG	1 cut(s)	116
Bsp1286I	GDGCHC	4 cut(s)	97, 208, 1627, 1861
BspACI	CCGC	5 cut(s)	50, 78, 124, 193, 327
BspANI	GGCC	8 cut(s)	241, 353, 479, 591, 748, 1406, 1937, 2006
BspCNI	CTCAG	6 cut(s)	119, 328, 742, 1858, 1952, 1975
BspDI	ATCGAT	2 cut(s)	909, 1300
BspFNI	CGCG	2 cut(s)	126, 2118
BspHI	TCATGA	2 cut(s)	135, 1686
BspMAI	CTGCAG	3 cut(s)	856, 1666, 1734
BspPI	GGATC	5 cut(s)	48, 192, 640, 765, 1044
BspT107I	GGYRCC	2 cut(s)	92, 323
BspTI	CTTAAG	1 cut(s)	563
BsrDI	GCAATG	1 cut(s)	153
BsrFI	RCCGGY	1 cut(s)	265
BsrI	ACTGG	6 cut(s)	201, 229, 325, 1018, 1036, 1854
BssAI	RCCGGY	1 cut(s)	265
BssECI	CCNNGG	8 cut(s)	217, 236, 342, 354, 592, 630, 1902, 2007
BssNI	GRCGYC	2 cut(s)	222, 1944
BssT1I	CCWWGG	4 cut(s)	236, 342, 592, 1902
Bst2UI	CCWGG	5 cut(s)	476, 1576, 1698, 2008, 2081
Bst4CI	ACNGT	4 cut(s)	606, 1055, 1616, 1863
Bst6I	CTCTTC	3 cut(s)	16, 1074, 1598
BstACI	GRCGYC	2 cut(s)	222, 1944
BstAFI	CTTAAG	1 cut(s)	563
BstAPI	GCANNNNNTGC	2 cut(s)	1274, 1439
BstC8I	GCNNGC	1 cut(s)	1260
BstDEI	CTNAG	9 cut(s)	106, 315, 750, 1088, 1157, 1866, 1939, 1962, 1995
BstDSI	CCRYGG	1 cut(s)	630
BstEII	GGTNACC	2 cut(s)	295, 382
BstENI	CCTNNNNNAGG	1 cut(s)	1379
BstFNI	CGCG	2 cut(s)	126, 2118
BstHHI	GCGC	1 cut(s)	2118
BstMWI	GCNNNNNNNGC	9 cut(s)	359, 739, 857, 938, 1259, 1268, 1274, 1322, 1439
BstNI	CCWGG	5 cut(s)	476, 1576, 1698, 2008, 2081
BstNSI	RCATGY	1 cut(s)	1277
BstPI	GGTNACC	2 cut(s)	295, 382
BstSCI	CCNGG	6 cut(s)	217, 474, 1574, 1696, 2006, 2079
BstSFI	CTRYAG	4 cut(s)	852, 1615, 1662, 1730
BstSLI	GKGCMC	3 cut(s)	97, 208, 1861
BstUI	CGCG	2 cut(s)	126, 2118
BstX2I	RGATCY	2 cut(s)	40, 645
BstXI	CCANNNNNNTGG	1 cut(s)	349
BstYI	RGATCY	2 cut(s)	40, 645
Bsu15I	ATCGAT	2 cut(s)	909, 1300
BsuI	GTATCC	1 cut(s)	1545
BsuRI	GGCC	8 cut(s)	241, 353, 479, 591, 748, 1406, 1937, 2006
BsuTUI	ATCGAT	2 cut(s)	909, 1300
BtgI	CCRYGG	1 cut(s)	630
BtrI	CACGTC	1 cut(s)	1889
BtsI	GCAGTG	1 cut(s)	810
BtsIMutI	CAGTG	7 cut(s)	208, 710, 810, 1011, 1131, 1859, 1861
Cac8I	GCNNGC	1 cut(s)	1260
CciI	TCATGA	2 cut(s)	135, 1686
CfoI	GCGC	1 cut(s)	2118
Cfr10I	RCCGGY	1 cut(s)	265
Cfr13I	GGNCC	6 cut(s)	73, 240, 1694, 1772, 1895, 2022
ClaI	ATCGAT	2 cut(s)	909, 1300
CseI	GACGC	2 cut(s)	163, 211
Csp6I	GTAC	1 cut(s)	1676
CspCI	CAANNNNNGTGG	2 cut(s)	136, 171
CviQI	GTAC	1 cut(s)	1676
DdeI	CTNAG	9 cut(s)	106, 315, 750, 1088, 1157, 1866, 1939, 1962, 1995
DraIII	CACNNNGTG	3 cut(s)	662, 1160, 1421
EaeI	YGGCCR	4 cut(s)	351, 477, 1404, 2004
Eam1104I	CTCTTC	3 cut(s)	16, 1074, 1598
EarI	CTCTTC	3 cut(s)	16, 1074, 1598
Eco130I	CCWWGG	4 cut(s)	236, 342, 592, 1902
Eco147I	AGGCCT	1 cut(s)	1937
Eco24I	GRGCYC	1 cut(s)	1627
Eco32I	GATATC	1 cut(s)	1959
Eco47I	GGWCC	5 cut(s)	73, 1694, 1772, 1895, 2022
Eco88I	CYCGRG	1 cut(s)	116
Eco91I	GGTNACC	2 cut(s)	295, 382
EcoNI	CCTNNNNNAGG	1 cut(s)	1379
EcoO109I	RGGNCCY	3 cut(s)	240, 1772, 1895
EcoO65I	GGTNACC	2 cut(s)	295, 382
EcoRI	GAATTC	2 cut(s)	1503, 1585
EcoRII	CCWGG	5 cut(s)	474, 1574, 1696, 2006, 2079
EcoRV	GATATC	1 cut(s)	1959
EcoT14I	CCWWGG	4 cut(s)	236, 342, 592, 1902
EcoT22I	ATGCAT	2 cut(s)	862, 1279
EcoT38I	GRGCYC	1 cut(s)	1627
ErhI	CCWWGG	4 cut(s)	236, 342, 592, 1902
FalI	AAGNNNNNCTT	8 cut(s)	371, 403, 672, 704, 1238, 1270, 1818, 1850
FaqI	GGGAC	2 cut(s)	1090, 1908
FauI	CCCGC	1 cut(s)	85
FauNDI	CATATG	2 cut(s)	433, 484
FbaI	TGATCA	1 cut(s)	700
FriOI	GRGCYC	1 cut(s)	1627
FspBI	CTAG	4 cut(s)	237, 1458, 1500, 1769
GlaI	GCGC	1 cut(s)	2117
GsaI	CCCAGC	2 cut(s)	1329, 1624
HaeIII	GGCC	8 cut(s)	241, 353, 479, 591, 748, 1406, 1937, 2006
HapII	CCGG	6 cut(s)	90, 219, 260, 266, 1704, 2020
HgaI	GACGC	2 cut(s)	163, 211
HhaI	GCGC	1 cut(s)	2118
Hin1I	GRCGYC	2 cut(s)	222, 1944
Hin6I	GCGC	1 cut(s)	2116
HinP1I	GCGC	1 cut(s)	2116
HinfI	GANTC	4 cut(s)	5, 568, 1060, 1927
HpaII	CCGG	6 cut(s)	90, 219, 260, 266, 1704, 2020
Hpy166II	GTNNAC	4 cut(s)	534, 1051, 1859, 2017
Hpy188I	TCNGA	9 cut(s)	45, 318, 973, 1242, 1282, 1368, 1867, 1932, 1942
Hpy8I	GTNNAC	4 cut(s)	534, 1051, 1859, 2017
Hpy99I	CGWCG	3 cut(s)	179, 182, 2123
HpyCH4III	ACNGT	4 cut(s)	606, 1055, 1616, 1863
HpyCH4IV	ACGT	5 cut(s)	1509, 1888, 1944, 1980, 2121
HpyF10VI	GCNNNNNNNGC	9 cut(s)	359, 739, 857, 938, 1259, 1268, 1274, 1322, 1439
HpyF3I	CTNAG	9 cut(s)	106, 315, 750, 1088, 1157, 1866, 1939, 1962, 1995
HpySE526I	ACGT	5 cut(s)	1509, 1888, 1944, 1980, 2121
Hsp92I	GRCGYC	2 cut(s)	222, 1944
HspAI	GCGC	1 cut(s)	2116
Ksp22I	TGATCA	1 cut(s)	700
LmnI	GCTCC	8 cut(s)	256, 262, 1172, 1630, 1717, 1760, 2044, 2069
LweI	GCATC	9 cut(s)	838, 847, 869, 901, 977, 1286, 1325, 1502, 1981
MaeI	CTAG	4 cut(s)	237, 1458, 1500, 1769
MaeII	ACGT	5 cut(s)	1509, 1888, 1944, 1980, 2121
MaeIII	GTNAC	5 cut(s)	100, 295, 382, 789, 1510
MfeI	CAATTG	1 cut(s)	1349
MflI	RGATCY	2 cut(s)	40, 645
MhlI	GDGCHC	4 cut(s)	97, 208, 1627, 1861
MlsI	TGGCCA	2 cut(s)	479, 2006
MluCI	AATT	7 cut(s)	840, 1071, 1349, 1394, 1503, 1585, 1629
MluNI	TGGCCA	2 cut(s)	479, 2006
MlyI	GAGTC	1 cut(s)	14
MmeI	TCCRAC	4 cut(s)	18, 735, 804, 1671
Mox20I	TGGCCA	2 cut(s)	479, 2006
Mph1103I	ATGCAT	2 cut(s)	862, 1279
MscI	TGGCCA	2 cut(s)	479, 2006
MseI	TTAA	9 cut(s)	564, 573, 678, 843, 1199, 1232, 1304, 1749, 1836
MslI	CAYNNNNRTG	2 cut(s)	1044, 2036
Msp20I	TGGCCA	2 cut(s)	479, 2006
MspA1I	CMGCKG	1 cut(s)	1325
MspCI	CTTAAG	1 cut(s)	563
MspI	CCGG	6 cut(s)	90, 219, 260, 266, 1704, 2020
MspR9I	CCNGG	6 cut(s)	219, 476, 1576, 1698, 2008, 2081
MunI	CAATTG	1 cut(s)	1349
Mva1269I	GAATGC	1 cut(s)	1880
MvaI	CCWGG	5 cut(s)	476, 1576, 1698, 2008, 2081
MvnI	CGCG	2 cut(s)	126, 2118
MwoI	GCNNNNNNNGC	9 cut(s)	359, 739, 857, 938, 1259, 1268, 1274, 1322, 1439
NciI	CCSGG	1 cut(s)	219
NdeI	CATATG	2 cut(s)	433, 484
NmeAIII	GCCGAG	1 cut(s)	379
NmuCI	GTSAC	4 cut(s)	100, 295, 382, 789
NsiI	ATGCAT	2 cut(s)	862, 1279
NspI	RCATGY	1 cut(s)	1277
OliI	CACNNNNGTG	1 cut(s)	2036
PaeR7I	CTCGAG	1 cut(s)	116
PagI	TCATGA	2 cut(s)	135, 1686
PceI	AGGCCT	1 cut(s)	1937
PcsI	WCGNNNNNNNCGW	2 cut(s)	123, 174
PctI	GAATGC	1 cut(s)	1880
PfeI	GAWTC	3 cut(s)	568, 1060, 1927
PflMI	CCANNNNNTGG	1 cut(s)	1160
PfoI	TCCNGGA	1 cut(s)	1696
PleI	GAGTC	1 cut(s)	13
PpsI	GAGTC	1 cut(s)	13
PpuMI	RGGWCCY	2 cut(s)	1772, 1895
Psp5II	RGGWCCY	2 cut(s)	1772, 1895
Psp6I	CCWGG	5 cut(s)	474, 1574, 1696, 2006, 2079
PspEI	GGTNACC	2 cut(s)	295, 382
PspFI	CCCAGC	2 cut(s)	1325, 1620
PspGI	CCWGG	5 cut(s)	474, 1574, 1696, 2006, 2079
PspPI	GGNCC	6 cut(s)	73, 240, 1694, 1772, 1895, 2022
PspPPI	RGGWCCY	2 cut(s)	1772, 1895
PstI	CTGCAG	3 cut(s)	856, 1666, 1734
PsuI	RGATCY	2 cut(s)	40, 645
PvuII	CAGCTG	1 cut(s)	1325
RsaI	GTAC	1 cut(s)	1677
RsaNI	GTAC	1 cut(s)	1676
RseI	CAYNNNNRTG	2 cut(s)	1044, 2036
SaqAI	TTAA	9 cut(s)	564, 573, 678, 843, 1199, 1232, 1304, 1749, 1836
Sau96I	GGNCC	6 cut(s)	73, 240, 1694, 1772, 1895, 2022
SbfI	CCTGCAGG	1 cut(s)	1734
SchI	GAGTC	1 cut(s)	14
ScrFI	CCNGG	6 cut(s)	219, 476, 1576, 1698, 2008, 2081
SdaI	CCTGCAGG	1 cut(s)	1734
SduI	GDGCHC	4 cut(s)	97, 208, 1627, 1861
SfaNI	GCATC	9 cut(s)	838, 847, 869, 901, 977, 1286, 1325, 1502, 1981
SfcI	CTRYAG	4 cut(s)	852, 1615, 1662, 1730
Sfr274I	CTCGAG	1 cut(s)	116
SinI	GGWCC	5 cut(s)	73, 1694, 1772, 1895, 2022
SlaI	CTCGAG	1 cut(s)	116
SmiMI	CAYNNNNRTG	2 cut(s)	1044, 2036
SmlI	CTYRAG	5 cut(s)	116, 563, 992, 1379, 1483
SmoI	CTYRAG	5 cut(s)	116, 563, 992, 1379, 1483
Sse8387I	CCTGCAGG	1 cut(s)	1734
Sse9I	AATT	7 cut(s)	840, 1071, 1349, 1394, 1503, 1585, 1629
SseBI	AGGCCT	1 cut(s)	1937
SsiI	CCGC	5 cut(s)	50, 78, 124, 193, 327
SspMI	CTAG	4 cut(s)	237, 1458, 1500, 1769
StuI	AGGCCT	1 cut(s)	1937
StyD4I	CCNGG	6 cut(s)	217, 474, 1574, 1696, 2006, 2079
StyI	CCWWGG	4 cut(s)	236, 342, 592, 1902
TaaI	ACNGT	4 cut(s)	606, 1055, 1616, 1863
TaiI	ACGT	5 cut(s)	1512, 1891, 1947, 1983, 2124
TaqI	TCGA	9 cut(s)	20, 117, 370, 769, 832, 909, 1124, 1216, 1300
TasI	AATT	7 cut(s)	840, 1071, 1349, 1394, 1503, 1585, 1629
TauI	GCSGC	2 cut(s)	53, 196
TfiI	GAWTC	3 cut(s)	568, 1060, 1927
Tru1I	TTAA	9 cut(s)	564, 573, 678, 843, 1199, 1232, 1304, 1749, 1836
Tru9I	TTAA	9 cut(s)	564, 573, 678, 843, 1199, 1232, 1304, 1749, 1836
TscAI	CASTG	7 cut(s)	208, 710, 817, 1018, 1138, 1861, 1866
TseFI	GTSAC	4 cut(s)	100, 295, 382, 789
Tsp45I	GTSAC	4 cut(s)	100, 295, 382, 789
TspDTI	ATGAA	6 cut(s)	38, 947, 1353, 1595, 1839, 2114
TspGWI	ACGGA	2 cut(s)	1579, 2118
TspRI	CASTG	7 cut(s)	208, 710, 817, 1018, 1138, 1861, 1866
Van91I	CCANNNNNTGG	1 cut(s)	1160
Vha464I	CTTAAG	1 cut(s)	563
VneI	GTGCAC	1 cut(s)	1857
VpaK11BI	GGWCC	5 cut(s)	73, 1694, 1772, 1895, 2022
XagI	CCTNNNNNAGG	1 cut(s)	1379
XapI	RAATTY	3 cut(s)	1394, 1503, 1585
XbaI	TCTAGA	1 cut(s)	1499
XceI	RCATGY	1 cut(s)	1277
XhoI	CTCGAG	1 cut(s)	116
XmaJI	CCTAGG	1 cut(s)	236
XspI	CTAG	4 cut(s)	237, 1458, 1500, 1769
ZraI	GACGTC	1 cut(s)	1945
Zsp2I	ATGCAT	2 cut(s)	862, 1279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.