Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

27194537 .. 27199723

Gene Structure 5187 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1253300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGGAGTCCTCTTCCAACTCGATGAAAATCTCACCCTTGGATCTGATGGCGGCAATCATCAAGGGCAAGATGGACCCCGCCAACGCCTCCGCCGACTTCTCCGGCGCCCAAGTCACCTCAGTCATTCTCGAGAACCGCGAGTTCGTCATGATCCTGACCACCTCCATTGCCGTCCTCATCGGCTGCGTCGTCGTTTTCGTCTTGCGGCGATTCAGTTCCCAGAAGCAGTCTCGAGTCCTCCAGCCTCCCAAGCCCCTCATCGTGCTGGAGCCGGAGCCGGAGGAGGTCGACGACGGCAGCAGGAAGGTCACCATCTTCTTCGGTACTCAGACTGGTACCGCCGAAGGCTTCGCCAAGGCTTTGGCCGAGGAAGCAAAAGCTCGATATGAGAAGGTCACATTCAAAGTTGTTGATTTGGATGATTACGCTGCCGATGACGAAGCATATGAGGAGAAATTGAAGAATGAGAGCTTGGCATTTTTCTTCCTGGCCACATATGGTGATGGTGAGCCAACCGACAACGCTGCGAGGTTTTACAAATGGTTTACAGAGGGTGAAGAGAGAGGGGAGTGGCTTAAGAATCTTCACTACGGAGTGTTTGGCCTCGGAAACAGGCAGTATGAGCATTTCAATAAGGTTGCCGTGGTTGTGGATGAGATCCTTGCTGCGCAAGGTGGGAAGCGACTTGTTAAAGTCGGTCTTGGAGATGATGATCAATGCATTGAGGATGACTTCTCTGCATGGCGAGAGTTGATGTGGCCCGAGTTGGATCAGTTGCTTCGAGATGAGGATGATGTCAAGTCACCTGTTTCTACACCTTACACTGCCGCTGTATTGGAGTATCGAGTTATAATTAAGGATGCTGCAGATGCATCAGTAGAAAGTAAGAGCTGGAATAATGCAAATGGTCATGCTGTCATTGATGCCCAACATCCATGCGGAGCTAATGTGGCTGTCAGAAGAGAACTTCATACTCCGGCATCTGATCGTTCTTGCACCCATCTCGAATTTGATATTTCTGGCACTGGACTTACATACGAAACTGGGGATCATGTTGGTGTTTACTGTGAGAATCTTCCTGAAGTTGTGGAAGAGGTGCTAAGTTTGTTGGGTTTGTCCCCAGAAATATACTTCTCGATACACACTGATAGAGAGGATGGCACACCACTAAGTGGAAGCTCCTTGACACCTCCCTTCCCACCATGCACACTAAGAACAGCACTAACTCAATATGCTGATGTTTTAAATGCTCCGAAAAAGTCTGCCTTACTCGCTTTAGCAGCACATGCATCTGACCCAACTGAAGCTGATCGATTAAGACATCTTGCATCGCCAGCTGGGAAGGATGAATATGCACAATGGGTGGTTGCAAGTCAGAGAAGCCTTCTTGAGGTCATGGCTGAATTTCCATCAGCCAAGCCTCCGCTTGGTGTCTTCTTCGCGGCAGTCGCTCCAAGATTACAGCCTAGATACTATTCAATCTCGTCATCCCCAAGGATGGCGTCTTCTAGAATTCACGTTACTTGTGCATTAGTTCATGAGAAAACACGAGCAGGAAGGATCCACAAAGGAGTGTGTTCAACCTGGATGAAGAATTCCATTCCTTTGGAGAAGAGCGATGACTGTAGCTGGGCACCCATTTTTGTTCGACAATCCAACTTCAAACTTCCTGCCGATACTAAAGTACCTATTCTTATGATTGGTCCTGGAACCGGATTTGCTCCATTCAGGGGTTTCCTGCAGGAAAGATTGGCTCTTAAAGAAGATGGAGCGGAATTGGGATCCTCCACATTATTCTTTGGATGTAGAAATAGTCAAATGGATTACATTTACGAAGATGAGCTGAACAACTTTTTAGCGACCGGTGCACTTTCTGAGCTGGTGGTTGCATTCTCACGTCAGGGACCTACCAAGGAATATGTGCAGCATAAAATGATGCAGAAGGCGTCAGACGTCTGGAACATGATATCTCAGGGAGGTTATATTTACGTTTGTGGTGATGCTAAGGGCATGGCCAGGGATGTCCACCGGACTCTTCACACAATCGTGCAGGAGCAGGGATGCATGGACAGCTCCAAAGCCGAAGGCCTGGTGAAAAATCTGCAAATGAACGGAAGATATCTGCGTGATGTGTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

713

Amino Acids

78.74

Weight (kDa)

5.22

Isoelectric Point (pI)

38.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	105 - 248	3e-32	Flavodoxin
FAD_binding_1	PF00667	308 - 529	4.9e-71	FAD binding domain
NAD_binding_1	PF00175	566 - 676	1.8e-15	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	851
AatII	GACGTC	1 cut(s)	1956
Acc16I	TGCGCA	1 cut(s)	669
Acc65I	GGTACC	1 cut(s)	335
AccB1I	GGYRCC	3 cut(s)	104, 335, 1633
AccB7I	CCANNNNNTGG	1 cut(s)	1172
AccBSI	CCGCTC	1 cut(s)	1772
AccI	GTMKAC	1 cut(s)	288
AccII	CGCG	2 cut(s)	138, 1442
AclWI	GGATC	9 cut(s)	48, 145, 652, 777, 1056, 1555, 1568, 1776, 1789
AcoI	YGGCCR	3 cut(s)	363, 489, 2013
AcsI	RAATTY	4 cut(s)	1007, 1403, 1512, 1594
AcuI	CTGAAG	2 cut(s)	1101, 1323
AcyI	GRCGYC	4 cut(s)	105, 1502, 1946, 1953
AdeI	CACNNNGTG	1 cut(s)	1172
AfaI	GTAC	3 cut(s)	325, 337, 1686
AfiI	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
AflII	CTTAAG	1 cut(s)	575
AgeI	ACCGGT	1 cut(s)	1862
AgsI	TTSAA	6 cut(s)	403, 460, 631, 1479, 1581, 1663
AjiI	CACGTC	1 cut(s)	1898
AjnI	CCWGG	5 cut(s)	486, 1583, 1705, 2015, 2088
AjuI	GAANNNNNNNTTGG	2 cut(s)	455, 487
AleI	CACNNNNGTG	1 cut(s)	2045
Alw21I	GWGCWC	1 cut(s)	1870
Alw26I	GTCTC	1 cut(s)	234
Alw44I	GTGCAC	1 cut(s)	1866
AlwI	GGATC	9 cut(s)	48, 145, 652, 777, 1056, 1555, 1568, 1776, 1789
Ama87I	CYCGRG	3 cut(s)	128, 231, 761
AoxI	GGCC	6 cut(s)	363, 489, 601, 758, 2013, 2086
ApaLI	GTGCAC	1 cut(s)	1866
ApeKI	GCWGC	8 cut(s)	183, 297, 428, 524, 665, 863, 1280, 1924
ApoI	RAATTY	4 cut(s)	1007, 1403, 1512, 1594
AsiGI	ACCGGT	1 cut(s)	1862
Asp718I	GGTACC	1 cut(s)	335
AspLEI	GCGC	2 cut(s)	107, 670
AspS9I	GGNCC	4 cut(s)	73, 759, 1703, 1904
AsuHPI	GGTGA	9 cut(s)	24, 106, 301, 512, 518, 566, 795, 2009, 2104
AvaI	CYCGRG	3 cut(s)	128, 231, 761
AvaII	GGWCC	3 cut(s)	73, 1703, 1904
BaeGI	GKGCMC	2 cut(s)	1636, 1870
BaeI	ACNNNNGTAYC	4 cut(s)	1668, 1668, 1701, 1701
BalI	TGGCCA	2 cut(s)	491, 2015
BamHI	GGATCC	2 cut(s)	1560, 1781
BanI	GGYRCC	3 cut(s)	104, 335, 1633
BauI	CACGAG	1 cut(s)	1548
BbsI	GAAGAC	2 cut(s)	1426, 1497
Bbv12I	GWGCWC	1 cut(s)	1870
BbvI	GCAGC	8 cut(s)	170, 309, 415, 511, 652, 850, 1292, 1936
BccI	CCATC	9 cut(s)	40, 64, 320, 497, 1008, 1151, 1417, 1492, 1760
BceAI	ACGGC	3 cut(s)	155, 310, 626
BcgI	CGANNNNNNTGC	4 cut(s)	506, 540, 985, 1019
BciT130I	CCWGG	5 cut(s)	488, 1585, 1707, 2017, 2090
BclI	TGATCA	1 cut(s)	712
BcoDI	GTCTC	1 cut(s)	234
BfaI	CTAG	2 cut(s)	1467, 1509
BfmI	CTRYAG	3 cut(s)	864, 1624, 1739
BfoI	RGCGCY	1 cut(s)	108
BfrI	CTTAAG	1 cut(s)	575
Bme1390I	CCNGG	5 cut(s)	488, 1585, 1707, 2017, 2090
Bme18I	GGWCC	3 cut(s)	73, 1703, 1904
BmeT110I	CYCGRG	3 cut(s)	128, 231, 761
BmgBI	CACGTC	1 cut(s)	1898
BmgT120I	GGNCC	4 cut(s)	73, 759, 1703, 1904
BmrFI	CCNGG	5 cut(s)	488, 1585, 1707, 2017, 2090
BmrI	ACTGGG	1 cut(s)	1053
BmuI	ACTGGG	1 cut(s)	1053
BpiI	GAAGAC	2 cut(s)	1426, 1497
BpmI	CTGGAG	2 cut(s)	224, 287
Bpu10I	CCTNAGC	1 cut(s)	2004
BpuEI	CTTGAG	1 cut(s)	1409
Bsa29I	ATCGAT	1 cut(s)	1312
BsaBI	GATNNNNATC	2 cut(s)	26, 711
BsaHI	GRCGYC	4 cut(s)	105, 1502, 1946, 1953
BsaJI	CCNNGG	8 cut(s)	36, 354, 366, 604, 642, 1493, 1911, 2016
BsaWI	WCCGGW	3 cut(s)	1712, 1862, 2028
BsaXI	ACNNNNNCTCC	2 cut(s)	933, 963
Bsc4I	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
Bse118I	RCCGGY	1 cut(s)	1862
Bse1I	ACTGG	3 cut(s)	337, 1030, 1048
Bse3DI	GCAATG	1 cut(s)	165
Bse8I	GATNNNNATC	2 cut(s)	26, 711
BseBI	CCWGG	5 cut(s)	488, 1585, 1707, 2017, 2090
BseCI	ATCGAT	1 cut(s)	1312
BseDI	CCNNGG	8 cut(s)	36, 354, 366, 604, 642, 1493, 1911, 2016
BseJI	GATNNNNATC	2 cut(s)	26, 711
BseLI	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
BseMI	GCAATG	1 cut(s)	165
BseMII	CTCAG	4 cut(s)	132, 341, 1866, 1985
BseNI	ACTGG	3 cut(s)	337, 1030, 1048
BseRI	GAGGAG	2 cut(s)	296, 464
BseSI	GKGCMC	2 cut(s)	1636, 1870
BseXI	GCAGC	8 cut(s)	170, 309, 415, 511, 652, 850, 1292, 1936
BseYI	CCCAGC	2 cut(s)	1337, 1629
BsgI	GTGCAG	2 cut(s)	1943, 2069
Bsh1236I	CGCG	2 cut(s)	138, 1442
Bsh1285I	CGRYCG	1 cut(s)	1863
BshFI	GGCC	6 cut(s)	365, 491, 603, 760, 2015, 2088
BshNI	GGYRCC	3 cut(s)	104, 335, 1633
BshTI	ACCGGT	1 cut(s)	1862
BshVI	ATCGAT	1 cut(s)	1312
BsiEI	CGRYCG	1 cut(s)	1863
BsiHKAI	GWGCWC	1 cut(s)	1870
BsiHKCI	CYCGRG	3 cut(s)	128, 231, 761
BsiSI	CCGG	7 cut(s)	102, 272, 278, 977, 1713, 1863, 2029
BslFI	GGGAC	2 cut(s)	1102, 1917
BslI	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
BsmAI	GTCTC	1 cut(s)	234
BsmFI	GGGAC	2 cut(s)	1102, 1917
BsmI	GAATGC	1 cut(s)	1889
BsnI	GGCC	6 cut(s)	365, 491, 603, 760, 2015, 2088
BsoBI	CYCGRG	3 cut(s)	128, 231, 761
Bsp1286I	GDGCHC	2 cut(s)	1636, 1870
BspANI	GGCC	6 cut(s)	365, 491, 603, 760, 2015, 2088
BspCNI	CTCAG	4 cut(s)	131, 340, 1867, 1984
BspDI	ATCGAT	1 cut(s)	1312
BspFNI	CGCG	2 cut(s)	138, 1442
BspHI	TCATGA	2 cut(s)	147, 1537
BspMAI	CTGCAG	2 cut(s)	868, 1743
BspPI	GGATC	9 cut(s)	48, 145, 652, 777, 1056, 1555, 1568, 1776, 1789
BspQI	GCTCTTC	1 cut(s)	1607
BspT107I	GGYRCC	3 cut(s)	104, 335, 1633
BspTI	CTTAAG	1 cut(s)	575
BsrBI	CCGCTC	1 cut(s)	1772
BsrDI	GCAATG	1 cut(s)	165
BsrFI	RCCGGY	1 cut(s)	1862
BsrI	ACTGG	3 cut(s)	337, 1030, 1048
BssAI	RCCGGY	1 cut(s)	1862
BssECI	CCNNGG	8 cut(s)	36, 354, 366, 604, 642, 1493, 1911, 2016
BssNI	GRCGYC	4 cut(s)	105, 1502, 1946, 1953
BssSI	CACGAG	1 cut(s)	1548
BssT1I	CCWWGG	4 cut(s)	36, 354, 1493, 1911
Bst2BI	CACGAG	1 cut(s)	1548
Bst2UI	CCWGG	5 cut(s)	488, 1585, 1707, 2017, 2090
Bst4CI	ACNGT	2 cut(s)	1067, 1625
Bst6I	CTCTTC	6 cut(s)	16, 552, 955, 1086, 1607, 2040
BstACI	GRCGYC	4 cut(s)	105, 1502, 1946, 1953
BstAFI	CTTAAG	1 cut(s)	575
BstAPI	GCANNNNNTGC	1 cut(s)	1286
BstC8I	GCNNGC	1 cut(s)	1335
BstDEI	CTNAG	8 cut(s)	118, 327, 1100, 1169, 1211, 1875, 1971, 2004
BstDSI	CCRYGG	1 cut(s)	642
BstEII	GGTNACC	1 cut(s)	307
BstENI	CCTNNNNNAGG	1 cut(s)	1388
BstFNI	CGCG	2 cut(s)	138, 1442
BstH2I	RGCGCY	1 cut(s)	108
BstHHI	GCGC	2 cut(s)	107, 670
BstMAI	GTCTC	1 cut(s)	234
BstMCI	CGRYCG	1 cut(s)	1863
BstNI	CCWGG	5 cut(s)	488, 1585, 1707, 2017, 2090
BstNSI	RCATGY	1 cut(s)	1289
BstPI	GGTNACC	1 cut(s)	307
BstSCI	CCNGG	5 cut(s)	486, 1583, 1705, 2015, 2088
BstSFI	CTRYAG	3 cut(s)	864, 1624, 1739
BstSLI	GKGCMC	2 cut(s)	1636, 1870
BstUI	CGCG	2 cut(s)	138, 1442
BstV1I	GCAGC	8 cut(s)	170, 309, 415, 511, 652, 850, 1292, 1936
BstV2I	GAAGAC	2 cut(s)	1426, 1497
BstX2I	RGATCY	4 cut(s)	40, 657, 1560, 1781
BstXI	CCANNNNNNTGG	2 cut(s)	361, 1606
BstYI	RGATCY	4 cut(s)	40, 657, 1560, 1781
Bsu15I	ATCGAT	1 cut(s)	1312
BsuRI	GGCC	6 cut(s)	365, 491, 603, 760, 2015, 2088
BsuTUI	ATCGAT	1 cut(s)	1312
BtgI	CCRYGG	1 cut(s)	642
BtgZI	GCGATG	2 cut(s)	1314, 1632
BtrI	CACGTC	1 cut(s)	1898
BtsI	GCAGTG	1 cut(s)	822
BtsIMutI	CAGTG	3 cut(s)	822, 1023, 1143
Cac8I	GCNNGC	1 cut(s)	1335
CciI	TCATGA	2 cut(s)	147, 1537
CfoI	GCGC	2 cut(s)	107, 670
Cfr10I	RCCGGY	1 cut(s)	1862
Cfr13I	GGNCC	4 cut(s)	73, 759, 1703, 1904
ClaI	ATCGAT	1 cut(s)	1312
CseI	GACGC	3 cut(s)	175, 1491, 1935
Csp6I	GTAC	3 cut(s)	324, 336, 1685
CspAI	ACCGGT	1 cut(s)	1862
CspCI	CAANNNNNGTGG	2 cut(s)	148, 183
CviQI	GTAC	3 cut(s)	324, 336, 1685
DdeI	CTNAG	8 cut(s)	118, 327, 1100, 1169, 1211, 1875, 1971, 2004
DinI	GGCGCC	1 cut(s)	106
DraI	TTTAAA	1 cut(s)	1245
DraIII	CACNNNGTG	1 cut(s)	1172
EaeI	YGGCCR	3 cut(s)	363, 489, 2013
Eam1104I	CTCTTC	6 cut(s)	16, 552, 955, 1086, 1607, 2040
EarI	CTCTTC	6 cut(s)	16, 552, 955, 1086, 1607, 2040
EciI	GGCGGA	1 cut(s)	79
Eco130I	CCWWGG	4 cut(s)	36, 354, 1493, 1911
Eco147I	AGGCCT	1 cut(s)	2088
Eco32I	GATATC	2 cut(s)	1968, 2120
Eco47I	GGWCC	3 cut(s)	73, 1703, 1904
Eco57I	CTGAAG	2 cut(s)	1101, 1323
Eco88I	CYCGRG	3 cut(s)	128, 231, 761
Eco91I	GGTNACC	1 cut(s)	307
EcoNI	CCTNNNNNAGG	1 cut(s)	1388
EcoO109I	RGGNCCY	1 cut(s)	1904
EcoO65I	GGTNACC	1 cut(s)	307
EcoRI	GAATTC	2 cut(s)	1512, 1594
EcoRII	CCWGG	5 cut(s)	486, 1583, 1705, 2015, 2088
EcoRV	GATATC	2 cut(s)	1968, 2120
EcoT14I	CCWWGG	4 cut(s)	36, 354, 1493, 1911
EcoT22I	ATGCAT	4 cut(s)	722, 874, 1291, 2066
EgeI	GGCGCC	1 cut(s)	106
EheI	GGCGCC	1 cut(s)	106
ErhI	CCWWGG	4 cut(s)	36, 354, 1493, 1911
FalI	AAGNNNNNCTT	4 cut(s)	684, 716, 1250, 1282
FaqI	GGGAC	2 cut(s)	1102, 1917
FauI	CCCGC	1 cut(s)	85
FauNDI	CATATG	2 cut(s)	445, 496
FbaI	TGATCA	1 cut(s)	712
FblI	GTMKAC	1 cut(s)	288
FspBI	CTAG	2 cut(s)	1467, 1509
FspI	TGCGCA	1 cut(s)	669
GlaI	GCGC	2 cut(s)	106, 669
GsaI	CCCAGC	2 cut(s)	1341, 1633
GsuI	CTGGAG	2 cut(s)	224, 287
HaeII	RGCGCY	1 cut(s)	108
HaeIII	GGCC	6 cut(s)	365, 491, 603, 760, 2015, 2088
HapII	CCGG	7 cut(s)	102, 272, 278, 977, 1713, 1863, 2029
HgaI	GACGC	3 cut(s)	175, 1491, 1935
HhaI	GCGC	2 cut(s)	107, 670
Hin1I	GRCGYC	4 cut(s)	105, 1502, 1946, 1953
Hin6I	GCGC	2 cut(s)	105, 668
HinP1I	GCGC	2 cut(s)	105, 668
HincII	GTYRAC	1 cut(s)	289
HindII	GTYRAC	1 cut(s)	289
HinfI	GANTC	6 cut(s)	5, 210, 234, 580, 1072, 2032
HpaII	CCGG	7 cut(s)	102, 272, 278, 977, 1713, 1863, 2029
HphI	GGTGA	9 cut(s)	24, 106, 301, 512, 518, 566, 795, 2009, 2104
Hpy166II	GTNNAC	5 cut(s)	289, 546, 1063, 1868, 2026
Hpy8I	GTNNAC	5 cut(s)	289, 546, 1063, 1868, 2026
Hpy99I	CGWCG	4 cut(s)	191, 194, 293, 296
HpyAV	CCTTC	9 cut(s)	298, 338, 385, 1204, 1336, 1394, 1551, 1936, 2078
HpyCH4III	ACNGT	2 cut(s)	1067, 1625
HpyCH4IV	ACGT	4 cut(s)	1518, 1897, 1953, 1989
HpyF3I	CTNAG	8 cut(s)	118, 327, 1100, 1169, 1211, 1875, 1971, 2004
HpySE526I	ACGT	4 cut(s)	1518, 1897, 1953, 1989
Hsp92I	GRCGYC	4 cut(s)	105, 1502, 1946, 1953
HspAI	GCGC	2 cut(s)	105, 668
KasI	GGCGCC	1 cut(s)	104
KpnI	GGTACC	1 cut(s)	339
Ksp22I	TGATCA	1 cut(s)	712
LguI	GCTCTTC	1 cut(s)	1607
Lsp1109I	GCAGC	8 cut(s)	170, 309, 415, 511, 652, 850, 1292, 1936
MaeI	CTAG	2 cut(s)	1467, 1509
MaeII	ACGT	4 cut(s)	1518, 1897, 1953, 1989
MaeIII	GTNAC	5 cut(s)	112, 307, 394, 801, 1519
MbiI	CCGCTC	1 cut(s)	1772
MflI	RGATCY	4 cut(s)	40, 657, 1560, 1781
MhlI	GDGCHC	2 cut(s)	1636, 1870
MlsI	TGGCCA	2 cut(s)	491, 2015
MluCI	AATT	7 cut(s)	455, 852, 1007, 1403, 1512, 1594, 1775
MluNI	TGGCCA	2 cut(s)	491, 2015
Mly113I	GGCGCC	1 cut(s)	105
MlyI	GAGTC	3 cut(s)	14, 243, 2026
MmeI	TCCRAC	3 cut(s)	39, 747, 1680
Mox20I	TGGCCA	2 cut(s)	491, 2015
Mph1103I	ATGCAT	4 cut(s)	722, 874, 1291, 2066
MscI	TGGCCA	2 cut(s)	491, 2015
MseI	TTAA	6 cut(s)	576, 690, 855, 1244, 1316, 1758
MslI	CAYNNNNRTG	2 cut(s)	1056, 2045
Msp20I	TGGCCA	2 cut(s)	491, 2015
MspA1I	CMGCKG	2 cut(s)	830, 1337
MspCI	CTTAAG	1 cut(s)	575
MspI	CCGG	7 cut(s)	102, 272, 278, 977, 1713, 1863, 2029
MspR9I	CCNGG	5 cut(s)	488, 1585, 1707, 2017, 2090
Mva1269I	GAATGC	1 cut(s)	1889
MvaI	CCWGG	5 cut(s)	488, 1585, 1707, 2017, 2090
MvnI	CGCG	2 cut(s)	138, 1442
NarI	GGCGCC	1 cut(s)	105
NdeI	CATATG	2 cut(s)	445, 496
NmeAIII	GCCGAG	1 cut(s)	391
NmuCI	GTSAC	4 cut(s)	112, 307, 394, 801
NsbI	TGCGCA	1 cut(s)	669
NsiI	ATGCAT	4 cut(s)	722, 874, 1291, 2066
NspI	RCATGY	1 cut(s)	1289
OliI	CACNNNNGTG	1 cut(s)	2045
PaeR7I	CTCGAG	2 cut(s)	128, 231
PagI	TCATGA	2 cut(s)	147, 1537
PceI	AGGCCT	1 cut(s)	2088
PciSI	GCTCTTC	1 cut(s)	1607
PcsI	WCGNNNNNNNCGW	4 cut(s)	90, 135, 186, 195
PctI	GAATGC	1 cut(s)	1889
PfeI	GAWTC	3 cut(s)	210, 580, 1072
PflMI	CCANNNNNTGG	1 cut(s)	1172
PfoI	TCCNGGA	1 cut(s)	1705
PinAI	ACCGGT	1 cut(s)	1862
PleI	GAGTC	3 cut(s)	13, 242, 2026
PluTI	GGCGCC	1 cut(s)	108
PpsI	GAGTC	3 cut(s)	13, 242, 2026
PpuMI	RGGWCCY	1 cut(s)	1904
PsiI	TTATAA	1 cut(s)	851
Psp5II	RGGWCCY	1 cut(s)	1904
Psp6I	CCWGG	5 cut(s)	486, 1583, 1705, 2015, 2088
PspEI	GGTNACC	1 cut(s)	307
PspFI	CCCAGC	2 cut(s)	1337, 1629
PspGI	CCWGG	5 cut(s)	486, 1583, 1705, 2015, 2088
PspPI	GGNCC	4 cut(s)	73, 759, 1703, 1904
PspPPI	RGGWCCY	1 cut(s)	1904
PstI	CTGCAG	2 cut(s)	868, 1743
PsuI	RGATCY	4 cut(s)	40, 657, 1560, 1781
PvuII	CAGCTG	1 cut(s)	1337
RsaI	GTAC	3 cut(s)	325, 337, 1686
RsaNI	GTAC	3 cut(s)	324, 336, 1685
RseI	CAYNNNNRTG	2 cut(s)	1056, 2045
SalI	GTCGAC	1 cut(s)	287
SapI	GCTCTTC	1 cut(s)	1607
SaqAI	TTAA	6 cut(s)	576, 690, 855, 1244, 1316, 1758
Sau96I	GGNCC	4 cut(s)	73, 759, 1703, 1904
SbfI	CCTGCAGG	1 cut(s)	1743
SchI	GAGTC	3 cut(s)	14, 243, 2026
ScrFI	CCNGG	5 cut(s)	488, 1585, 1707, 2017, 2090
SdaI	CCTGCAGG	1 cut(s)	1743
SduI	GDGCHC	2 cut(s)	1636, 1870
SfcI	CTRYAG	3 cut(s)	864, 1624, 1739
SfoI	GGCGCC	1 cut(s)	106
Sfr274I	CTCGAG	2 cut(s)	128, 231
SinI	GGWCC	3 cut(s)	73, 1703, 1904
SlaI	CTCGAG	2 cut(s)	128, 231
SmiMI	CAYNNNNRTG	2 cut(s)	1056, 2045
SmlI	CTYRAG	4 cut(s)	128, 231, 575, 1388
SmoI	CTYRAG	4 cut(s)	128, 231, 575, 1388
Sse8387I	CCTGCAGG	1 cut(s)	1743
Sse9I	AATT	7 cut(s)	455, 852, 1007, 1403, 1512, 1594, 1775
SseBI	AGGCCT	1 cut(s)	2088
SspDI	GGCGCC	1 cut(s)	104
SspMI	CTAG	2 cut(s)	1467, 1509
StuI	AGGCCT	1 cut(s)	2088
StyD4I	CCNGG	5 cut(s)	486, 1583, 1705, 2015, 2088
StyI	CCWWGG	4 cut(s)	36, 354, 1493, 1911
TaaI	ACNGT	2 cut(s)	1067, 1625
TaiI	ACGT	4 cut(s)	1521, 1900, 1956, 1992
TaqII	GACCGA	1 cut(s)	686
TasI	AATT	7 cut(s)	455, 852, 1007, 1403, 1512, 1594, 1775
TauI	GCSGC	4 cut(s)	53, 208, 830, 1445
TfiI	GAWTC	3 cut(s)	210, 580, 1072
Tru1I	TTAA	6 cut(s)	576, 690, 855, 1244, 1316, 1758
Tru9I	TTAA	6 cut(s)	576, 690, 855, 1244, 1316, 1758
TscAI	CASTG	3 cut(s)	829, 1030, 1150
TseFI	GTSAC	4 cut(s)	112, 307, 394, 801
TseI	GCWGC	8 cut(s)	183, 297, 428, 524, 665, 863, 1280, 1924
Tsp45I	GTSAC	4 cut(s)	112, 307, 394, 801
TspDTI	ATGAA	6 cut(s)	38, 959, 1362, 1526, 1604, 2123
TspGWI	ACGGA	2 cut(s)	606, 2127
TspRI	CASTG	3 cut(s)	829, 1030, 1150
Van91I	CCANNNNNTGG	1 cut(s)	1172
Vha464I	CTTAAG	1 cut(s)	575
VneI	GTGCAC	1 cut(s)	1866
VpaK11BI	GGWCC	3 cut(s)	73, 1703, 1904
XagI	CCTNNNNNAGG	1 cut(s)	1388
XapI	RAATTY	4 cut(s)	1007, 1403, 1512, 1594
XbaI	TCTAGA	1 cut(s)	1508
XceI	RCATGY	1 cut(s)	1289
XhoI	CTCGAG	2 cut(s)	128, 231
XmiI	GTMKAC	1 cut(s)	288
XspI	CTAG	2 cut(s)	1467, 1509
ZraI	GACGTC	1 cut(s)	1954
Zsp2I	ATGCAT	4 cut(s)	722, 874, 1291, 2066

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.