Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

24311861 .. 24316984

Gene Structure 5124 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG112600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2049 bp

ATGGACCCCTCCAACGCCTCCGCCGAGGTCACCTCCGTCATTCTCGACAATCGCGACTTCGTTATGATCCTCACGACCTCGATTGCCGTTTTGATCGGCTGCGTCGTCGTTTTTGTGTGGCGGCGATCGAGCGCTCAGAAGCCCAGGGTCGTCCAGCCTCTTAAGCCGTTGATCATCGCGGAGCCTGAGCCGGAGCTCGACGATGGAAGCAAGAAGGTCACAATCTTCTTCGGCACGCAAACCGGTACTGCCGAGGGATTTGCCAAGGCTTTTGCTGAAGAAGCGAAAGCTCGCTATGAAAAGGTCACATTCAAAGTTGTTGATTTGGACGATTATGCAGCTGATGACGAGGCGTATGAGGAGAAATTGAAAAATGAGAGTCTGGCCATTTTCTTTTTGGCGACATATGGTGATGGCGAGCCGACGGATAATGCTGCTAGATTTTACAAGTGGTTTACAGAGGGTAAAGAGAAGGAAGCATGGCTTCAAAATCTTCATTACGGAGTGTTTGGTCTTGGAAACAGGCAGTATGAGCATTTCAACAAGGTGGCCGTGGTTGTGGATGAGTTCCTCGCTGCTCAGGGTGGGAAGCGACTTGTTCCAGTGGGCCTTGGAGATGATGATCAATGCATAGAGGATGATTTCACTGCATGGCGAGAGTTGATGTGGCCTGAGTTGGACCAGTTGCTTCGAGATGAGGATGATGCAACCACACCTGCTTGCACACCTTACACTGCTGCTGTCTTGGAGTATCGAGTTGTATTTAATGATGCTGCTGATGTATCAGTAGAGGATAAGAGCTGGAATACTGCAAATGGTCATGCTGTTATTGATGCTCAGCATCCATGCAGGGCTAATGTTGCTGTGAGAAAGGAACTTCATACTCCTGCATCTGATCGTTCTTGCACCCATCTCGAATTTGATATTGCTGGCACTGGACTTGCATACGAAACTGGTGATCATGTCGGTGTGTACTGTGAGAATCTTTTTGAAACTGTGGAAGAGGCAATAAGTTTGTTGGGCTTGGCACCGGAAACATACTTCTCGATGCACACTGATAAGGATGATGGTACACCACTTAGTGGAGGCTCGTTGTTGCCTCCCTTTCCACCGTGCACATTAAGAACAGCACTAACTCGATATGCTGATCTTTTGAGTTCTCCGAAAAAGTCTGCCCTGCTGGCTTTAGCTGCCTATGCATCTGATCCAGCTGAAGCGGATCGATTAAGACATCTTGCATCACCTGCTGGGAAGGATGAATATGCTCAGTGGATGGTTGCGAGTCAGAGAAGCCTTCTTGAGGTCATGGCTGAATTTCCATCAGCCAAGCCCCCACTTGGAGTCTTCTTTGCAGCAGTTGCTCCAAGGTTGCAGCCTAGATACTATTCAATTTCGTCATCCCCGAGGATGGCACCTTCTAGAATTCATGTTACTTGTGCACTAGTTTATGACAAAACATCAACAGGAAGGATTCACAAAGGAGTGTGTTCAACCTGGATGAAGAATTCTGTGCCTTCCGCAAAAAGTGATGATTGTAGTTGGGCACCCGTTTTTGTTCGGCAATCAAACTTCAGACTTCCCGTTGATACTGAAGTACCTATTCTTATGATTGGTCCCGGAACTGGATTGGCTCCTTTCAGGGGCTTCCTGCAGGAAAGATTAGCTCTTAAAGAAGATGGAGCAGAACTTGGACCCTCCGTATTATTCTTTGGATGCAGAAATAGTCAAATGGACTACATTTATGAAGATGAGCTGAACAACTTTGTAGAAAAAGGTGCACTTTCTGAGCTTGTGGTTGTGTTCTCCCGCCAGGGACCCACTAAGGAATATGTGCAACATAAAATGATGCAGAAGGCTTCAGACATCTGGAACATGCTATCTCAGGGAGGTTATATTTACGTTTGTGGTGATGCTAAGGGCATGGCCAGGGATGTCCACCGGACTCTTCACACAATCGTGCAGGAGCAGGGATCTCTGGACAGCTCCAAAGCTGAGAGCATGGTGAAAAATCTGCAAATGAATGGCAGGTATTTGCGTGATGTGTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

682

Amino Acids

75.59

Weight (kDa)

5.03

Isoelectric Point (pI)

38.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	75 - 218	2.5e-32	Flavodoxin
FAD_binding_1	PF00667	278 - 499	7.6e-70	FAD binding domain
NAD_binding_1	PF00175	536 - 646	3.1e-16	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	724, 1252
Acc36I	ACCTGC	3 cut(s)	724, 1252, 2016
AccB1I	GGYRCC	3 cut(s)	1027, 1411, 1543
AccB7I	CCANNNNNTGG	1 cut(s)	1082
AccII	CGCG	2 cut(s)	54, 179
AciI	CCGC	6 cut(s)	21, 121, 179, 1217, 1518, 1807
AclWI	GGATC	4 cut(s)	61, 1199, 1227, 1978
AcoI	YGGCCR	3 cut(s)	384, 549, 1923
AcsI	RAATTY	4 cut(s)	917, 1313, 1422, 1504
AcuI	CTGAAG	5 cut(s)	297, 1233, 1555, 1611, 1842
AdeI	CACNNNGTG	1 cut(s)	1082
AfaI	GTAC	4 cut(s)	247, 974, 1072, 1596
AfeI	AGCGCT	1 cut(s)	133
AfiI	CCNNNNNNNGG	6 cut(s)	1082, 1300, 1337, 1408, 1622, 1640
AflII	CTTAAG	1 cut(s)	161
AgeI	ACCGGT	1 cut(s)	242
AgsI	TTSAA	7 cut(s)	313, 370, 488, 541, 992, 1389, 1491
AhlI	ACTAGT	1 cut(s)	1441
AjnI	CCWGG	4 cut(s)	143, 1493, 1809, 1925
AleI	CACNNNNGTG	1 cut(s)	1955
Alw21I	GWGCWC	4 cut(s)	198, 1118, 1441, 1780
Alw44I	GTGCAC	3 cut(s)	1114, 1437, 1776
AlwI	GGATC	4 cut(s)	61, 1199, 1227, 1978
Ama87I	CYCGRG	1 cut(s)	1402
Aor51HI	AGCGCT	1 cut(s)	133
AoxI	GGCC	5 cut(s)	384, 549, 607, 668, 1923
ApaLI	GTGCAC	3 cut(s)	1114, 1437, 1776
ApeKI	GCWGC	9 cut(s)	99, 338, 434, 575, 737, 773, 1190, 1352, 1372
ApoI	RAATTY	4 cut(s)	917, 1313, 1422, 1504
AsiGI	ACCGGT	1 cut(s)	242
AspLEI	GCGC	1 cut(s)	134
AspS9I	GGNCC	6 cut(s)	4, 607, 679, 1613, 1691, 1814
AsuC2I	CCSGG	1 cut(s)	1617
AsuHPI	GGTGA	6 cut(s)	22, 422, 968, 1233, 1919, 2014
AvaI	CYCGRG	1 cut(s)	1402
AvaII	GGWCC	5 cut(s)	4, 679, 1613, 1691, 1814
BaeGI	GKGCMC	4 cut(s)	1118, 1441, 1546, 1780
BaeI	ACNNNNGTAYC	4 cut(s)	1578, 1578, 1611, 1611
BalI	TGGCCA	2 cut(s)	386, 1925
BanI	GGYRCC	3 cut(s)	1027, 1411, 1543
BanII	GRGCYC	1 cut(s)	198
BbsI	GAAGAC	1 cut(s)	1336
Bbv12I	GWGCWC	4 cut(s)	198, 1118, 1441, 1780
BbvI	GCAGC	9 cut(s)	86, 350, 421, 562, 724, 760, 1177, 1364, 1384
BccI	CCATC	8 cut(s)	197, 407, 918, 1061, 1267, 1327, 1402, 1670
BceAI	ACGGC	3 cut(s)	71, 151, 536
BcgI	CGANNNNNNTGC	4 cut(s)	242, 276, 895, 929
BciT130I	CCWGG	4 cut(s)	145, 1495, 1811, 1927
BclI	TGATCA	3 cut(s)	171, 622, 958
BcnI	CCSGG	1 cut(s)	1617
BcuI	ACTAGT	1 cut(s)	1441
BfaI	CTAG	4 cut(s)	438, 1377, 1419, 1442
BfmI	CTRYAG	1 cut(s)	1649
BfoI	RGCGCY	1 cut(s)	135
BfrI	CTTAAG	1 cut(s)	161
BfuAI	ACCTGC	3 cut(s)	724, 1252, 2016
BglI	GCCNNNNNGGC	1 cut(s)	1181
BlpI	GCTNAGC	1 cut(s)	837
Bme1390I	CCNGG	5 cut(s)	145, 1495, 1617, 1811, 1927
Bme18I	GGWCC	5 cut(s)	4, 679, 1613, 1691, 1814
BmeT110I	CYCGRG	1 cut(s)	1402
BmgT120I	GGNCC	6 cut(s)	4, 607, 679, 1613, 1691, 1814
BmrFI	CCNGG	5 cut(s)	145, 1495, 1617, 1811, 1927
BpiI	GAAGAC	1 cut(s)	1336
BplI	GAGNNNNNCTC	2 cut(s)	17, 49
Bpu10I	CCTNAGC	3 cut(s)	186, 579, 1914
Bpu1102I	GCTNAGC	1 cut(s)	837
BpuEI	CTTGAG	1 cut(s)	1319
BpuMI	CCSGG	1 cut(s)	1617
Bsa29I	ATCGAT	1 cut(s)	1222
BsaBI	GATNNNNATC	1 cut(s)	621
BsaWI	WCCGGW	3 cut(s)	242, 1030, 1938
Bsc4I	CCNNNNNNNGG	6 cut(s)	1082, 1300, 1337, 1408, 1622, 1640
Bse118I	RCCGGY	1 cut(s)	242
Bse1I	ACTGG	5 cut(s)	602, 682, 940, 958, 1627
Bse8I	GATNNNNATC	1 cut(s)	621
BseBI	CCWGG	4 cut(s)	145, 1495, 1811, 1927
BseCI	ATCGAT	1 cut(s)	1222
BseJI	GATNNNNATC	1 cut(s)	621
BseLI	CCNNNNNNNGG	6 cut(s)	1082, 1300, 1337, 1408, 1622, 1640
BseMII	CTCAG	9 cut(s)	149, 177, 593, 663, 851, 1280, 1776, 1895, 1983
BseNI	ACTGG	5 cut(s)	602, 682, 940, 958, 1627
BseRI	GAGGAG	1 cut(s)	374
BseSI	GKGCMC	4 cut(s)	1118, 1441, 1546, 1780
BseXI	GCAGC	9 cut(s)	86, 350, 421, 562, 724, 760, 1177, 1364, 1384
BseYI	CCCAGC	1 cut(s)	1247
BsgI	GTGCAG	1 cut(s)	1979
Bsh1236I	CGCG	2 cut(s)	54, 179
Bsh1285I	CGRYCG	1 cut(s)	128
BshFI	GGCC	5 cut(s)	386, 551, 609, 670, 1925
BshNI	GGYRCC	3 cut(s)	1027, 1411, 1543
BshTI	ACCGGT	1 cut(s)	242
BshVI	ATCGAT	1 cut(s)	1222
BsiEI	CGRYCG	1 cut(s)	128
BsiHKAI	GWGCWC	4 cut(s)	198, 1118, 1441, 1780
BsiHKCI	CYCGRG	1 cut(s)	1402
BsiSI	CCGG	5 cut(s)	191, 243, 1031, 1617, 1939
BslFI	GGGAC	2 cut(s)	1599, 1827
BslI	CCNNNNNNNGG	6 cut(s)	1082, 1300, 1337, 1408, 1622, 1640
BsmFI	GGGAC	2 cut(s)	1599, 1827
BsnI	GGCC	5 cut(s)	386, 551, 609, 670, 1925
BsoBI	CYCGRG	1 cut(s)	1402
Bsp1286I	GDGCHC	5 cut(s)	198, 1118, 1441, 1546, 1780
Bsp1720I	GCTNAGC	1 cut(s)	837
Bsp68I	TCGCGA	1 cut(s)	54
BspACI	CCGC	6 cut(s)	21, 121, 179, 1217, 1518, 1807
BspANI	GGCC	5 cut(s)	386, 551, 609, 670, 1925
BspCNI	CTCAG	9 cut(s)	148, 178, 592, 664, 850, 1279, 1777, 1894, 1984
BspDI	ATCGAT	1 cut(s)	1222
BspFNI	CGCG	2 cut(s)	54, 179
BspMAI	CTGCAG	1 cut(s)	1653
BspMI	ACCTGC	3 cut(s)	724, 1252, 2016
BspPI	GGATC	4 cut(s)	61, 1199, 1227, 1978
BspT107I	GGYRCC	3 cut(s)	1027, 1411, 1543
BspTI	CTTAAG	1 cut(s)	161
BsrFI	RCCGGY	1 cut(s)	242
BsrI	ACTGG	5 cut(s)	602, 682, 940, 958, 1627
BssAI	RCCGGY	1 cut(s)	242
BssT1I	CCWWGG	3 cut(s)	264, 610, 1364
Bst2UI	CCWGG	4 cut(s)	145, 1495, 1811, 1927
Bst4CI	ACNGT	3 cut(s)	977, 997, 1113
Bst6I	CTCTTC	2 cut(s)	996, 1950
BstAFI	CTTAAG	1 cut(s)	161
BstAPI	GCANNNNNTGC	2 cut(s)	1244, 1358
BstC8I	GCNNGC	6 cut(s)	236, 292, 419, 721, 931, 1182
BstDSI	CCRYGG	1 cut(s)	552
BstEII	GGTNACC	1 cut(s)	28
BstENI	CCTNNNNNAGG	1 cut(s)	1298
BstFNI	CGCG	2 cut(s)	54, 179
BstH2I	RGCGCY	1 cut(s)	135
BstHHI	GCGC	1 cut(s)	134
BstMCI	CGRYCG	1 cut(s)	128
BstMWI	GCNNNNNNNGC	9 cut(s)	163, 281, 532, 860, 1181, 1190, 1196, 1244, 1358
BstNI	CCWGG	4 cut(s)	145, 1495, 1811, 1927
BstNSI	RCATGY	1 cut(s)	1876
BstPI	GGTNACC	1 cut(s)	28
BstSCI	CCNGG	5 cut(s)	143, 1493, 1615, 1809, 1925
BstSFI	CTRYAG	1 cut(s)	1649
BstSLI	GKGCMC	4 cut(s)	1118, 1441, 1546, 1780
BstUI	CGCG	2 cut(s)	54, 179
BstV1I	GCAGC	9 cut(s)	86, 350, 421, 562, 724, 760, 1177, 1364, 1384
BstV2I	GAAGAC	1 cut(s)	1336
BstX2I	RGATCY	1 cut(s)	1970
BstYI	RGATCY	1 cut(s)	1970
Bsu15I	ATCGAT	1 cut(s)	1222
BsuRI	GGCC	5 cut(s)	386, 551, 609, 670, 1925
BsuTUI	ATCGAT	1 cut(s)	1222
BtgI	CCRYGG	1 cut(s)	552
BtgZI	GCGATG	1 cut(s)	160
BtsI	GCAGTG	2 cut(s)	645, 732
BtsIMutI	CAGTG	6 cut(s)	609, 645, 732, 933, 1053, 1274
BtuMI	TCGCGA	1 cut(s)	54
BveI	ACCTGC	3 cut(s)	724, 1252, 2016
Cac8I	GCNNGC	6 cut(s)	236, 292, 419, 721, 931, 1182
CfoI	GCGC	1 cut(s)	134
Cfr10I	RCCGGY	1 cut(s)	242
Cfr13I	GGNCC	6 cut(s)	4, 607, 679, 1613, 1691, 1814
ClaI	ATCGAT	1 cut(s)	1222
CseI	GACGC	1 cut(s)	91
Csp6I	GTAC	4 cut(s)	246, 973, 1071, 1595
CspAI	ACCGGT	1 cut(s)	242
CviQI	GTAC	4 cut(s)	246, 973, 1071, 1595
DraIII	CACNNNGTG	1 cut(s)	1082
EaeI	YGGCCR	3 cut(s)	384, 549, 1923
Eam1104I	CTCTTC	2 cut(s)	996, 1950
EarI	CTCTTC	2 cut(s)	996, 1950
EciI	GGCGGA	1 cut(s)	10
Ecl136II	GAGCTC	1 cut(s)	196
Eco130I	CCWWGG	3 cut(s)	264, 610, 1364
Eco24I	GRGCYC	1 cut(s)	198
Eco47I	GGWCC	5 cut(s)	4, 679, 1613, 1691, 1814
Eco47III	AGCGCT	1 cut(s)	133
Eco53kI	GAGCTC	1 cut(s)	196
Eco57I	CTGAAG	5 cut(s)	297, 1233, 1555, 1611, 1842
Eco88I	CYCGRG	1 cut(s)	1402
Eco91I	GGTNACC	1 cut(s)	28
EcoICRI	GAGCTC	1 cut(s)	196
EcoNI	CCTNNNNNAGG	1 cut(s)	1298
EcoO109I	RGGNCCY	1 cut(s)	1814
EcoO65I	GGTNACC	1 cut(s)	28
EcoRI	GAATTC	2 cut(s)	1422, 1504
EcoRII	CCWGG	4 cut(s)	143, 1493, 1809, 1925
EcoT14I	CCWWGG	3 cut(s)	264, 610, 1364
EcoT22I	ATGCAT	2 cut(s)	632, 1201
EcoT38I	GRGCYC	1 cut(s)	198
ErhI	CCWWGG	3 cut(s)	264, 610, 1364
FalI	AAGNNNNNCTT	4 cut(s)	468, 500, 1764, 1796
FaqI	GGGAC	2 cut(s)	1599, 1827
FauI	CCCGC	1 cut(s)	1814
FauNDI	CATATG	1 cut(s)	406
FbaI	TGATCA	3 cut(s)	171, 622, 958
FriOI	GRGCYC	1 cut(s)	198
FspBI	CTAG	4 cut(s)	438, 1377, 1419, 1442
GlaI	GCGC	1 cut(s)	133
GsaI	CCCAGC	1 cut(s)	1251
HaeII	RGCGCY	1 cut(s)	135
HaeIII	GGCC	5 cut(s)	386, 551, 609, 670, 1925
HapII	CCGG	5 cut(s)	191, 243, 1031, 1617, 1939
HgaI	GACGC	1 cut(s)	91
HhaI	GCGC	1 cut(s)	134
Hin6I	GCGC	1 cut(s)	132
HinP1I	GCGC	1 cut(s)	132
HinfI	GANTC	6 cut(s)	379, 982, 1282, 1341, 1471, 1942
HpaII	CCGG	5 cut(s)	191, 243, 1031, 1617, 1939
HphI	GGTGA	6 cut(s)	22, 422, 968, 1233, 1919, 2014
Hpy166II	GTNNAC	7 cut(s)	456, 973, 1073, 1116, 1439, 1778, 1936
Hpy188I	TCNGA	8 cut(s)	138, 895, 1164, 1204, 1287, 1574, 1786, 1861
Hpy8I	GTNNAC	7 cut(s)	456, 973, 1073, 1116, 1439, 1778, 1936
Hpy99I	CGWCG	4 cut(s)	107, 110, 203, 427
HpyAV	CCTTC	8 cut(s)	208, 466, 1246, 1304, 1425, 1461, 1524, 1846
HpyCH4III	ACNGT	3 cut(s)	977, 997, 1113
HpyCH4IV	ACGT	1 cut(s)	1899
HpyF10VI	GCNNNNNNNGC	9 cut(s)	163, 281, 532, 860, 1181, 1190, 1196, 1244, 1358
HpySE526I	ACGT	1 cut(s)	1899
HspAI	GCGC	1 cut(s)	132
KflI	GGGWCCC	1 cut(s)	1814
Ksp22I	TGATCA	3 cut(s)	171, 622, 958
LmnI	GCTCC	7 cut(s)	181, 193, 1366, 1636, 1679, 1963, 1988
Lsp1109I	GCAGC	9 cut(s)	86, 350, 421, 562, 724, 760, 1177, 1364, 1384
MaeI	CTAG	4 cut(s)	438, 1377, 1419, 1442
MaeII	ACGT	1 cut(s)	1899
MaeIII	GTNAC	4 cut(s)	28, 217, 304, 1429
MflI	RGATCY	1 cut(s)	1970
MhlI	GDGCHC	5 cut(s)	198, 1118, 1441, 1546, 1780
MlsI	TGGCCA	2 cut(s)	386, 1925
MluCI	AATT	6 cut(s)	365, 917, 1313, 1389, 1422, 1504
MluNI	TGGCCA	2 cut(s)	386, 1925
MlyI	GAGTC	4 cut(s)	388, 1291, 1350, 1936
MmeI	TCCRAC	2 cut(s)	36, 657
Mox20I	TGGCCA	2 cut(s)	386, 1925
Mph1103I	ATGCAT	2 cut(s)	632, 1201
MscI	TGGCCA	2 cut(s)	386, 1925
MseI	TTAA	5 cut(s)	162, 765, 1121, 1226, 1668
MslI	CAYNNNNRTG	2 cut(s)	966, 1955
Msp20I	TGGCCA	2 cut(s)	386, 1925
MspA1I	CMGCKG	2 cut(s)	341, 1211
MspCI	CTTAAG	1 cut(s)	161
MspI	CCGG	5 cut(s)	191, 243, 1031, 1617, 1939
MspR9I	CCNGG	5 cut(s)	145, 1495, 1617, 1811, 1927
MvaI	CCWGG	4 cut(s)	145, 1495, 1811, 1927
MvnI	CGCG	2 cut(s)	54, 179
MwoI	GCNNNNNNNGC	9 cut(s)	163, 281, 532, 860, 1181, 1190, 1196, 1244, 1358
NciI	CCSGG	1 cut(s)	1617
NdeI	CATATG	1 cut(s)	406
NmeAIII	GCCGAG	2 cut(s)	49, 277
NmuCI	GTSAC	3 cut(s)	28, 217, 304
NruI	TCGCGA	1 cut(s)	54
NsiI	ATGCAT	2 cut(s)	632, 1201
NspI	RCATGY	1 cut(s)	1876
OliI	CACNNNNGTG	1 cut(s)	1955
PaqCI	CACCTGC	2 cut(s)	724, 1252
PasI	CCCWGGG	1 cut(s)	144
PcsI	WCGNNNNNNNCGW	4 cut(s)	21, 51, 102, 1400
PfeI	GAWTC	2 cut(s)	982, 1471
PflMI	CCANNNNNTGG	1 cut(s)	1082
PfoI	TCCNGGA	1 cut(s)	1615
PinAI	ACCGGT	1 cut(s)	242
Ple19I	CGATCG	1 cut(s)	128
PleI	GAGTC	4 cut(s)	387, 1290, 1349, 1936
PpsI	GAGTC	4 cut(s)	387, 1290, 1349, 1936
PpuMI	RGGWCCY	1 cut(s)	1814
Psp124BI	GAGCTC	1 cut(s)	198
Psp5II	RGGWCCY	1 cut(s)	1814
Psp6I	CCWGG	4 cut(s)	143, 1493, 1809, 1925
PspEI	GGTNACC	1 cut(s)	28
PspFI	CCCAGC	1 cut(s)	1247
PspGI	CCWGG	4 cut(s)	143, 1493, 1809, 1925
PspPI	GGNCC	6 cut(s)	4, 607, 679, 1613, 1691, 1814
PspPPI	RGGWCCY	1 cut(s)	1814
PstI	CTGCAG	1 cut(s)	1653
PsuI	RGATCY	1 cut(s)	1970
PvuI	CGATCG	1 cut(s)	128
PvuII	CAGCTG	2 cut(s)	341, 1211
RruI	TCGCGA	1 cut(s)	54
RsaI	GTAC	4 cut(s)	247, 974, 1072, 1596
RsaNI	GTAC	4 cut(s)	246, 973, 1071, 1595
RseI	CAYNNNNRTG	2 cut(s)	966, 1955
SacI	GAGCTC	1 cut(s)	198
SaqAI	TTAA	5 cut(s)	162, 765, 1121, 1226, 1668
Sau96I	GGNCC	6 cut(s)	4, 607, 679, 1613, 1691, 1814
SbfI	CCTGCAGG	1 cut(s)	1653
SchI	GAGTC	4 cut(s)	388, 1291, 1350, 1936
ScrFI	CCNGG	5 cut(s)	145, 1495, 1617, 1811, 1927
SdaI	CCTGCAGG	1 cut(s)	1653
SduI	GDGCHC	5 cut(s)	198, 1118, 1441, 1546, 1780
SfcI	CTRYAG	1 cut(s)	1649
SinI	GGWCC	5 cut(s)	4, 679, 1613, 1691, 1814
SmiMI	CAYNNNNRTG	2 cut(s)	966, 1955
SmlI	CTYRAG	2 cut(s)	161, 1298
SmoI	CTYRAG	2 cut(s)	161, 1298
SpeI	ACTAGT	1 cut(s)	1441
Sse8387I	CCTGCAGG	1 cut(s)	1653
Sse9I	AATT	6 cut(s)	365, 917, 1313, 1389, 1422, 1504
SsiI	CCGC	6 cut(s)	21, 121, 179, 1217, 1518, 1807
SspMI	CTAG	4 cut(s)	438, 1377, 1419, 1442
SstI	GAGCTC	1 cut(s)	198
StyD4I	CCNGG	5 cut(s)	143, 1493, 1615, 1809, 1925
StyI	CCWWGG	3 cut(s)	264, 610, 1364
TaaI	ACNGT	3 cut(s)	977, 997, 1113
TaiI	ACGT	1 cut(s)	1902
TasI	AATT	6 cut(s)	365, 917, 1313, 1389, 1422, 1504
TatI	WGTACW	1 cut(s)	972
TauI	GCSGC	1 cut(s)	124
TfiI	GAWTC	2 cut(s)	982, 1471
Tru1I	TTAA	5 cut(s)	162, 765, 1121, 1226, 1668
Tru9I	TTAA	5 cut(s)	162, 765, 1121, 1226, 1668
TscAI	CASTG	6 cut(s)	609, 652, 739, 940, 1060, 1274
TseFI	GTSAC	3 cut(s)	28, 217, 304
TseI	GCWGC	9 cut(s)	99, 338, 434, 575, 737, 773, 1190, 1352, 1372
Tsp45I	GTSAC	3 cut(s)	28, 217, 304
TspDTI	ATGAA	8 cut(s)	312, 485, 869, 1272, 1415, 1514, 1758, 2033
TspGWI	ACGGA	4 cut(s)	25, 440, 516, 1687
TspRI	CASTG	6 cut(s)	609, 652, 739, 940, 1060, 1274
Van91I	CCANNNNNTGG	1 cut(s)	1082
Vha464I	CTTAAG	1 cut(s)	161
VneI	GTGCAC	3 cut(s)	1114, 1437, 1776
VpaK11BI	GGWCC	5 cut(s)	4, 679, 1613, 1691, 1814
XagI	CCTNNNNNAGG	1 cut(s)	1298
XapI	RAATTY	4 cut(s)	917, 1313, 1422, 1504
XbaI	TCTAGA	1 cut(s)	1418
XceI	RCATGY	1 cut(s)	1876
XcmI	CCANNNNNNNNNTGG	1 cut(s)	394
XspI	CTAG	4 cut(s)	438, 1377, 1419, 1442
Zsp2I	ATGCAT	2 cut(s)	632, 1201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.