Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

24298330 .. 24303564

Gene Structure 5235 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG112500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2139 bp

ATGGAGTCGACTTCCGATAGCTCGATGAAAATCTCGCCGTTGGATCTGATGGCGGCGATCTTCAAGGGCAAGATGGACCCCTCCAACGCCTCCGCTGCCTCCTCTGCCGGCGCCGAGGTCGCCTCCGTCATTCTCGAGAATCGTGAGTTCGTTATGATCCTGACGACCTCGATTGCCATTTTGATTGGCTGCGTCGTCGTTTTTGTGTGGCGGCGCTCGAGTGCTCAGAAGCCCAAGGTTGTCCAGCCTCTTAAGCCGTTGGTCATCACGGAGCCTGAGCCGGAGCTCGACGATGGAAGTAAGAAGGTCACAATCTTCTTCGGCACGCAAACCGGTACTGCTGAGGGATTTGCCAAGGCTTTGGCTGAGGAAGCGAAAGCTCGCTATGAAAAGGTCACATTCAAAGTTGTTGATTTGGATGATTACGCAGCTGATGACGAGGAGTATGAGGAGAAATTGAAAAATGAGAGTTTGGCAATTTTCTTCTTGGCCACATATGGTGATGGTGAGCCGACGGATAATGCTGCTAGATTTTACAAGTGGTTTACAGAGGGTAAAGAGAAAGAAGCATGGCTTCAAAATCTTCATTACGGAGTGTTTGGTCTTGGAAACAGGCAGTATGAGCATTTCAATAAGGTGGCCGTGGTTGTGGATGAGATCCTTGCTGCTCAGGGTGGGAAGAGACTTGTTTCAGTGGGCCTTGGAGATGATGATCAATGCATAGAGGATGATTTCACTGCATGGCGAGAGTCGATGTGGCCTGAGTTGGACCAGTTGCTTCGAGATGAGGATGATGCAACCACACCTGCTTGCACACCTTACACTGCTGCTGTCTTGGAGTATCGAGTTGTATTTAATGATGCTGCTGATGCACCAGTAGAGGATAAGAGCTGGAATAATGCAAACGGTCATGCTGTCATTGATGCTCAGCATCCATGCAGGGCTAATGTTGCTGTGAGAAAGGAACTTCATACTCCTGCATCTGATCGTTCTTGCACGCATCTCGAATTTGATATTGCTGGCACTGGACTTATATATGAAACTGGTGATCATGTCGGTGTGTACTGTGAGAATCTTTCTGAAACTGTGGAAGAGGCGATTAGTTTGTTGGGCTTTGCTCCGGAAACATACTTCTCGATGCACACTGATAAGGATGATGGTACACCACTTAGCGGAGGCTCCTTGTTACCTCCCTTTCCACCGTGCACATTAAGAACAGCACTAACTCGATATGCTGATCTTTTGAGTTCTCCGAAAAAGTCTGCCCTACTGGCTTTAGCTGCCTATGCATCTGATCCAGCTGAAGCAGATCGATTAAGACATCTTGCATCACCTGCTGGGAAGGATGAATATGCTCAATGGATGGTTGCAAGTCAGAGAAGCCTTCTTGAGGTCATGGCTGAATTTCCATCAGCCAAGCCCCCACTTGGAGTCTTCTTTGCAGCAGTTGCTCCAAGATTGCAGCCTAGATACTATTCAATCTCGTCATCCCCGAGGATGGCACCATCTAGAATTCATGTTACTTGTGCACTAGTTTATGACAAAACATCAACAGGAAGGATTCACAAAGGAGTGTGTTCAACCTGGATGAAGAATTCTGTGCCTTCAGCAAAAAGTGATGATTGTAGTTGGGCACCCGTTTTTGTTCGGCAATCAAACTTCAGACTTCCCGTTGATACTAAAGTACCTATTCTTATGATTGGTCCTGGAACTGGATTGGCTCCTTTCAGGGGTTTCCTGCAGGAAAGATTAGCGCTTAAAGAAGATGGAGCAGAACTTGGACCCTCCGTATTATTCTTTGGATGCAGAAATAGTCAAATGGACTACATTTACGAAGATGAGCTGAGCAACTTTGTAGAAAAAGGTGCACTTTCTGAGCTTGTGCTTGCATTCTCTCGCCAGGGACCCACCAAGGAATATGTGCAACATAAAATGATGCAGAAGGCGTCAGACATCTGGAATATGCTATCTCAGGGAGGTTATATTTACGTTTGTGGTGACGCTAAGGGCATGGCCAGGGATGTCCACCGAACTCTTCACACAATCGTGCAGGAGCAGGGATCTCTGGACAGCTCAAAAGCTGAGAGCATGGTGAAAAATCTGCAAATGAATGGCAGGTATTTGCGTGATGTGTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

712

Amino Acids

78.53

Weight (kDa)

5.08

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	105 - 248	1.5e-32	Flavodoxin
FAD_binding_1	PF00667	308 - 529	1.2e-69	FAD binding domain
NAD_binding_1	PF00175	566 - 676	6.2e-16	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	814, 1342
Acc36I	ACCTGC	3 cut(s)	814, 1342, 2106
AccB1I	GGYRCC	3 cut(s)	110, 1501, 1633
AccI	GTMKAC	1 cut(s)	8
AccIII	TCCGGA	1 cut(s)	1120
AciI	CCGC	4 cut(s)	53, 93, 211, 1173
AclWI	GGATC	5 cut(s)	51, 151, 652, 1289, 2068
AcoI	YGGCCR	3 cut(s)	489, 639, 2013
AcsI	RAATTY	4 cut(s)	1007, 1403, 1512, 1594
AcuI	CTGAAG	3 cut(s)	1323, 1590, 1645
AcyI	GRCGYC	2 cut(s)	111, 1946
AfaI	GTAC	4 cut(s)	337, 1064, 1162, 1686
AfeI	AGCGCT	1 cut(s)	1755
AfiI	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
AflII	CTTAAG	1 cut(s)	251
AgeI	ACCGGT	1 cut(s)	332
AgsI	TTSAA	7 cut(s)	64, 403, 460, 578, 631, 1479, 1581
AhlI	ACTAGT	1 cut(s)	1531
AjnI	CCWGG	4 cut(s)	1583, 1705, 1899, 2015
AleI	CACNNNNGTG	1 cut(s)	2045
Alw21I	GWGCWC	5 cut(s)	226, 288, 1208, 1531, 1870
Alw26I	GTCTC	1 cut(s)	676
Alw44I	GTGCAC	3 cut(s)	1204, 1527, 1866
AlwI	GGATC	5 cut(s)	51, 151, 652, 1289, 2068
Ama87I	CYCGRG	3 cut(s)	134, 217, 1492
Aor13HI	TCCGGA	1 cut(s)	1120
Aor51HI	AGCGCT	1 cut(s)	1755
AoxI	GGCC	5 cut(s)	489, 639, 697, 758, 2013
ApaLI	GTGCAC	3 cut(s)	1204, 1527, 1866
ApoI	RAATTY	4 cut(s)	1007, 1403, 1512, 1594
AsiGI	ACCGGT	1 cut(s)	332
AspLEI	GCGC	3 cut(s)	113, 216, 1756
AspS9I	GGNCC	6 cut(s)	76, 697, 769, 1703, 1781, 1904
AsuHPI	GGTGA	6 cut(s)	512, 518, 1058, 1323, 2009, 2104
AvaI	CYCGRG	3 cut(s)	134, 217, 1492
AvaII	GGWCC	5 cut(s)	76, 769, 1703, 1781, 1904
BaeGI	GKGCMC	4 cut(s)	1208, 1531, 1636, 1870
BaeI	ACNNNNGTAYC	4 cut(s)	1668, 1668, 1701, 1701
BalI	TGGCCA	2 cut(s)	491, 2015
BanI	GGYRCC	3 cut(s)	110, 1501, 1633
BanII	GRGCYC	1 cut(s)	288
BbsI	GAAGAC	1 cut(s)	1426
Bbv12I	GWGCWC	5 cut(s)	226, 288, 1208, 1531, 1870
BbvCI	CCTCAGC	2 cut(s)	342, 366
BceAI	ACGGC	3 cut(s)	22, 241, 626
BcgI	CGANNNNNNTGC	2 cut(s)	985, 1019
BciT130I	CCWGG	4 cut(s)	1585, 1707, 1901, 2017
BclI	TGATCA	2 cut(s)	712, 1048
BcoDI	GTCTC	1 cut(s)	676
BcuI	ACTAGT	1 cut(s)	1531
BfaI	CTAG	4 cut(s)	528, 1467, 1509, 1532
BfmI	CTRYAG	1 cut(s)	1739
BfoI	RGCGCY	3 cut(s)	114, 217, 1757
BfrI	CTTAAG	1 cut(s)	251
BfuAI	ACCTGC	3 cut(s)	814, 1342, 2106
BglI	GCCNNNNNGGC	1 cut(s)	1271
BlpI	GCTNAGC	2 cut(s)	927, 1844
Bme1390I	CCNGG	4 cut(s)	1585, 1707, 1901, 2017
Bme18I	GGWCC	5 cut(s)	76, 769, 1703, 1781, 1904
BmeT110I	CYCGRG	3 cut(s)	134, 217, 1492
BmgT120I	GGNCC	6 cut(s)	76, 697, 769, 1703, 1781, 1904
BmrFI	CCNGG	4 cut(s)	1585, 1707, 1901, 2017
BpiI	GAAGAC	1 cut(s)	1426
BplI	GAGNNNNNCTC	2 cut(s)	107, 139
Bpu10I	CCTNAGC	5 cut(s)	276, 342, 366, 669, 2004
Bpu1102I	GCTNAGC	2 cut(s)	927, 1844
BpuEI	CTTGAG	1 cut(s)	1409
Bsa29I	ATCGAT	1 cut(s)	1312
BsaBI	GATNNNNATC	2 cut(s)	29, 711
BsaHI	GRCGYC	2 cut(s)	111, 1946
BsaJI	CCNNGG	9 cut(s)	114, 234, 354, 642, 700, 1493, 1900, 1911, 2016
BsaWI	WCCGGW	2 cut(s)	332, 1120
Bsc4I	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
Bse118I	RCCGGY	2 cut(s)	107, 332
Bse1I	ACTGG	6 cut(s)	772, 875, 1030, 1048, 1275, 1717
Bse8I	GATNNNNATC	2 cut(s)	29, 711
BseAI	TCCGGA	1 cut(s)	1120
BseBI	CCWGG	4 cut(s)	1585, 1707, 1901, 2017
BseCI	ATCGAT	1 cut(s)	1312
BseDI	CCNNGG	9 cut(s)	114, 234, 354, 642, 700, 1493, 1900, 1911, 2016
BseJI	GATNNNNATC	2 cut(s)	29, 711
BseLI	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
BseNI	ACTGG	6 cut(s)	772, 875, 1030, 1048, 1275, 1717
BseRI	GAGGAG	3 cut(s)	91, 455, 464
BseSI	GKGCMC	4 cut(s)	1208, 1531, 1636, 1870
BseYI	CCCAGC	1 cut(s)	1337
BsgI	GTGCAG	1 cut(s)	2069
BshFI	GGCC	5 cut(s)	491, 641, 699, 760, 2015
BshNI	GGYRCC	3 cut(s)	110, 1501, 1633
BshTI	ACCGGT	1 cut(s)	332
BshVI	ATCGAT	1 cut(s)	1312
BsiHKAI	GWGCWC	5 cut(s)	226, 288, 1208, 1531, 1870
BsiHKCI	CYCGRG	3 cut(s)	134, 217, 1492
BsiSI	CCGG	4 cut(s)	108, 281, 333, 1121
BslFI	GGGAC	1 cut(s)	1917
BslI	CCNNNNNNNGG	6 cut(s)	1172, 1390, 1427, 1498, 1712, 1730
BsmAI	GTCTC	1 cut(s)	676
BsmFI	GGGAC	1 cut(s)	1917
BsmI	GAATGC	1 cut(s)	1889
BsnI	GGCC	5 cut(s)	491, 641, 699, 760, 2015
BsoBI	CYCGRG	3 cut(s)	134, 217, 1492
Bsp1286I	GDGCHC	6 cut(s)	226, 288, 1208, 1531, 1636, 1870
Bsp13I	TCCGGA	1 cut(s)	1120
Bsp1720I	GCTNAGC	2 cut(s)	927, 1844
BspACI	CCGC	4 cut(s)	53, 93, 211, 1173
BspANI	GGCC	5 cut(s)	491, 641, 699, 760, 2015
BspDI	ATCGAT	1 cut(s)	1312
BspEI	TCCGGA	1 cut(s)	1120
BspMAI	CTGCAG	1 cut(s)	1743
BspMI	ACCTGC	3 cut(s)	814, 1342, 2106
BspPI	GGATC	5 cut(s)	51, 151, 652, 1289, 2068
BspT107I	GGYRCC	3 cut(s)	110, 1501, 1633
BspTI	CTTAAG	1 cut(s)	251
BsrFI	RCCGGY	2 cut(s)	107, 332
BsrI	ACTGG	6 cut(s)	772, 875, 1030, 1048, 1275, 1717
BssAI	RCCGGY	2 cut(s)	107, 332
BssECI	CCNNGG	9 cut(s)	114, 234, 354, 642, 700, 1493, 1900, 1911, 2016
BssNI	GRCGYC	2 cut(s)	111, 1946
BssT1I	CCWWGG	4 cut(s)	234, 354, 700, 1911
Bst2UI	CCWGG	4 cut(s)	1585, 1707, 1901, 2017
Bst4CI	ACNGT	4 cut(s)	908, 1067, 1087, 1203
Bst6I	CTCTTC	3 cut(s)	674, 1086, 2040
BstACI	GRCGYC	2 cut(s)	111, 1946
BstAFI	CTTAAG	1 cut(s)	251
BstAPI	GCANNNNNTGC	2 cut(s)	1334, 1448
BstC8I	GCNNGC	7 cut(s)	109, 326, 382, 811, 998, 1021, 1887
BstDSI	CCRYGG	1 cut(s)	642
BstENI	CCTNNNNNAGG	1 cut(s)	1388
BstH2I	RGCGCY	3 cut(s)	114, 217, 1757
BstHHI	GCGC	3 cut(s)	113, 216, 1756
BstMAI	GTCTC	1 cut(s)	676
BstNI	CCWGG	4 cut(s)	1585, 1707, 1901, 2017
BstSCI	CCNGG	4 cut(s)	1583, 1705, 1899, 2015
BstSFI	CTRYAG	1 cut(s)	1739
BstSLI	GKGCMC	4 cut(s)	1208, 1531, 1636, 1870
BstV2I	GAAGAC	1 cut(s)	1426
BstX2I	RGATCY	3 cut(s)	43, 657, 2060
BstXI	CCANNNNNNTGG	1 cut(s)	361
BstYI	RGATCY	3 cut(s)	43, 657, 2060
Bsu15I	ATCGAT	1 cut(s)	1312
BsuRI	GGCC	5 cut(s)	491, 641, 699, 760, 2015
BsuTUI	ATCGAT	1 cut(s)	1312
BtgI	CCRYGG	1 cut(s)	642
BtsI	GCAGTG	2 cut(s)	735, 822
BtsIMutI	CAGTG	5 cut(s)	699, 735, 822, 1023, 1143
BveI	ACCTGC	3 cut(s)	814, 1342, 2106
Cac8I	GCNNGC	7 cut(s)	109, 326, 382, 811, 998, 1021, 1887
CfoI	GCGC	3 cut(s)	113, 216, 1756
Cfr10I	RCCGGY	2 cut(s)	107, 332
Cfr13I	GGNCC	6 cut(s)	76, 697, 769, 1703, 1781, 1904
ClaI	ATCGAT	1 cut(s)	1312
CseI	GACGC	3 cut(s)	181, 1935, 2009
Csp6I	GTAC	4 cut(s)	336, 1063, 1161, 1685
CspAI	ACCGGT	1 cut(s)	332
CviAII	CATG	9 cut(s)	570, 741, 911, 936, 1052, 1396, 1517, 2011, 2089
CviQI	GTAC	4 cut(s)	336, 1063, 1161, 1685
DinI	GGCGCC	1 cut(s)	112
EaeI	YGGCCR	3 cut(s)	489, 639, 2013
Eam1104I	CTCTTC	3 cut(s)	674, 1086, 2040
EarI	CTCTTC	3 cut(s)	674, 1086, 2040
Ecl136II	GAGCTC	1 cut(s)	286
Eco130I	CCWWGG	4 cut(s)	234, 354, 700, 1911
Eco24I	GRGCYC	1 cut(s)	288
Eco47I	GGWCC	5 cut(s)	76, 769, 1703, 1781, 1904
Eco47III	AGCGCT	1 cut(s)	1755
Eco53kI	GAGCTC	1 cut(s)	286
Eco57I	CTGAAG	3 cut(s)	1323, 1590, 1645
Eco88I	CYCGRG	3 cut(s)	134, 217, 1492
EcoICRI	GAGCTC	1 cut(s)	286
EcoNI	CCTNNNNNAGG	1 cut(s)	1388
EcoO109I	RGGNCCY	1 cut(s)	1904
EcoRI	GAATTC	2 cut(s)	1512, 1594
EcoRII	CCWGG	4 cut(s)	1583, 1705, 1899, 2015
EcoT14I	CCWWGG	4 cut(s)	234, 354, 700, 1911
EcoT22I	ATGCAT	2 cut(s)	722, 1291
EcoT38I	GRGCYC	1 cut(s)	288
EgeI	GGCGCC	1 cut(s)	112
EheI	GGCGCC	1 cut(s)	112
ErhI	CCWWGG	4 cut(s)	234, 354, 700, 1911
FaeI	CATG	9 cut(s)	573, 744, 914, 939, 1055, 1399, 1520, 2014, 2092
FalI	AAGNNNNNCTT	4 cut(s)	558, 590, 1854, 1886
FaqI	GGGAC	1 cut(s)	1917
FatI	CATG	9 cut(s)	569, 740, 910, 935, 1051, 1395, 1516, 2010, 2088
FauNDI	CATATG	1 cut(s)	496
FbaI	TGATCA	2 cut(s)	712, 1048
FblI	GTMKAC	1 cut(s)	8
FriOI	GRGCYC	1 cut(s)	288
FspBI	CTAG	4 cut(s)	528, 1467, 1509, 1532
GlaI	GCGC	3 cut(s)	112, 215, 1755
GsaI	CCCAGC	1 cut(s)	1341
HaeII	RGCGCY	3 cut(s)	114, 217, 1757
HaeIII	GGCC	5 cut(s)	491, 641, 699, 760, 2015
HapII	CCGG	4 cut(s)	108, 281, 333, 1121
HgaI	GACGC	3 cut(s)	181, 1935, 2009
HhaI	GCGC	3 cut(s)	113, 216, 1756
Hin1I	GRCGYC	2 cut(s)	111, 1946
Hin1II	CATG	9 cut(s)	573, 744, 914, 939, 1055, 1399, 1520, 2014, 2092
Hin6I	GCGC	3 cut(s)	111, 214, 1754
HinP1I	GCGC	3 cut(s)	111, 214, 1754
HincII	GTYRAC	1 cut(s)	9
HindII	GTYRAC	1 cut(s)	9
HinfI	GANTC	6 cut(s)	5, 139, 749, 1072, 1431, 1561
HpaII	CCGG	4 cut(s)	108, 281, 333, 1121
HphI	GGTGA	6 cut(s)	512, 518, 1058, 1323, 2009, 2104
Hpy166II	GTNNAC	8 cut(s)	9, 546, 1063, 1163, 1206, 1529, 1868, 2026
Hpy8I	GTNNAC	8 cut(s)	9, 546, 1063, 1163, 1206, 1529, 1868, 2026
Hpy99I	CGWCG	4 cut(s)	197, 200, 293, 517
HpyAV	CCTTC	6 cut(s)	298, 1336, 1394, 1551, 1614, 1936
HpyCH4III	ACNGT	4 cut(s)	908, 1067, 1087, 1203
HpyCH4IV	ACGT	1 cut(s)	1989
HpySE526I	ACGT	1 cut(s)	1989
Hsp92I	GRCGYC	2 cut(s)	111, 1946
Hsp92II	CATG	9 cut(s)	573, 744, 914, 939, 1055, 1399, 1520, 2014, 2092
HspAI	GCGC	3 cut(s)	111, 214, 1754
KasI	GGCGCC	1 cut(s)	110
KflI	GGGWCCC	1 cut(s)	1904
Kpn2I	TCCGGA	1 cut(s)	1120
KroI	GCCGGC	1 cut(s)	107
KroNI	GCCGGC	1 cut(s)	109
Ksp22I	TGATCA	2 cut(s)	712, 1048
LmnI	GCTCC	8 cut(s)	271, 283, 1123, 1184, 1456, 1726, 1769, 2053
MaeI	CTAG	4 cut(s)	528, 1467, 1509, 1532
MaeII	ACGT	1 cut(s)	1989
MaeIII	GTNAC	5 cut(s)	307, 394, 1185, 1519, 1997
MflI	RGATCY	3 cut(s)	43, 657, 2060
MhlI	GDGCHC	6 cut(s)	226, 288, 1208, 1531, 1636, 1870
MlsI	TGGCCA	2 cut(s)	491, 2015
MluCI	AATT	6 cut(s)	455, 477, 1007, 1403, 1512, 1594
MluNI	TGGCCA	2 cut(s)	491, 2015
Mly113I	GGCGCC	1 cut(s)	111
MlyI	GAGTC	3 cut(s)	14, 758, 1440
MmeI	TCCRAC	3 cut(s)	21, 108, 747
Mox20I	TGGCCA	2 cut(s)	491, 2015
Mph1103I	ATGCAT	2 cut(s)	722, 1291
MroI	TCCGGA	1 cut(s)	1120
MroNI	GCCGGC	1 cut(s)	107
MscI	TGGCCA	2 cut(s)	491, 2015
MseI	TTAA	5 cut(s)	252, 855, 1211, 1316, 1758
MslI	CAYNNNNRTG	2 cut(s)	1056, 2045
Msp20I	TGGCCA	2 cut(s)	491, 2015
MspA1I	CMGCKG	3 cut(s)	95, 431, 1301
MspCI	CTTAAG	1 cut(s)	251
MspI	CCGG	4 cut(s)	108, 281, 333, 1121
MspR9I	CCNGG	4 cut(s)	1585, 1707, 1901, 2017
Mva1269I	GAATGC	1 cut(s)	1889
MvaI	CCWGG	4 cut(s)	1585, 1707, 1901, 2017
NaeI	GCCGGC	1 cut(s)	109
NarI	GGCGCC	1 cut(s)	111
NdeI	CATATG	1 cut(s)	496
NgoMIV	GCCGGC	1 cut(s)	107
NlaIII	CATG	9 cut(s)	573, 744, 914, 939, 1055, 1399, 1520, 2014, 2092
NmeAIII	GCCGAG	1 cut(s)	139
NmuCI	GTSAC	3 cut(s)	307, 394, 1997
NsiI	ATGCAT	2 cut(s)	722, 1291
OliI	CACNNNNGTG	1 cut(s)	2045
PaeR7I	CTCGAG	2 cut(s)	134, 217
PaqCI	CACCTGC	2 cut(s)	814, 1342
PcsI	WCGNNNNNNNCGW	1 cut(s)	1490
PctI	GAATGC	1 cut(s)	1889
PdiI	GCCGGC	1 cut(s)	109
PfeI	GAWTC	3 cut(s)	139, 1072, 1561
PfoI	TCCNGGA	1 cut(s)	1705
PinAI	ACCGGT	1 cut(s)	332
PleI	GAGTC	3 cut(s)	13, 757, 1439
PluTI	GGCGCC	1 cut(s)	114
PpsI	GAGTC	3 cut(s)	13, 757, 1439
PpuMI	RGGWCCY	1 cut(s)	1904
Psp124BI	GAGCTC	1 cut(s)	288
Psp5II	RGGWCCY	1 cut(s)	1904
Psp6I	CCWGG	4 cut(s)	1583, 1705, 1899, 2015
PspFI	CCCAGC	1 cut(s)	1337
PspGI	CCWGG	4 cut(s)	1583, 1705, 1899, 2015
PspPI	GGNCC	6 cut(s)	76, 697, 769, 1703, 1781, 1904
PspPPI	RGGWCCY	1 cut(s)	1904
PspXI	VCTCGAGB	1 cut(s)	217
PstI	CTGCAG	1 cut(s)	1743
PsuI	RGATCY	3 cut(s)	43, 657, 2060
PvuII	CAGCTG	2 cut(s)	431, 1301
RsaI	GTAC	4 cut(s)	337, 1064, 1162, 1686
RsaNI	GTAC	4 cut(s)	336, 1063, 1161, 1685
RseI	CAYNNNNRTG	2 cut(s)	1056, 2045
SacI	GAGCTC	1 cut(s)	288
SalI	GTCGAC	1 cut(s)	7
SaqAI	TTAA	5 cut(s)	252, 855, 1211, 1316, 1758
Sau96I	GGNCC	6 cut(s)	76, 697, 769, 1703, 1781, 1904
SbfI	CCTGCAGG	1 cut(s)	1743
SchI	GAGTC	3 cut(s)	14, 758, 1440
ScrFI	CCNGG	4 cut(s)	1585, 1707, 1901, 2017
SdaI	CCTGCAGG	1 cut(s)	1743
SduI	GDGCHC	6 cut(s)	226, 288, 1208, 1531, 1636, 1870
SfcI	CTRYAG	1 cut(s)	1739
SfoI	GGCGCC	1 cut(s)	112
Sfr274I	CTCGAG	2 cut(s)	134, 217
SinI	GGWCC	5 cut(s)	76, 769, 1703, 1781, 1904
SlaI	CTCGAG	2 cut(s)	134, 217
SmiMI	CAYNNNNRTG	2 cut(s)	1056, 2045
SmlI	CTYRAG	4 cut(s)	134, 217, 251, 1388
SmoI	CTYRAG	4 cut(s)	134, 217, 251, 1388
SpeI	ACTAGT	1 cut(s)	1531
Sse8387I	CCTGCAGG	1 cut(s)	1743
Sse9I	AATT	6 cut(s)	455, 477, 1007, 1403, 1512, 1594
SsiI	CCGC	4 cut(s)	53, 93, 211, 1173
SspDI	GGCGCC	1 cut(s)	110
SspMI	CTAG	4 cut(s)	528, 1467, 1509, 1532
SstI	GAGCTC	1 cut(s)	288
StyD4I	CCNGG	4 cut(s)	1583, 1705, 1899, 2015
StyI	CCWWGG	4 cut(s)	234, 354, 700, 1911
TaaI	ACNGT	4 cut(s)	908, 1067, 1087, 1203
TaiI	ACGT	1 cut(s)	1992
TasI	AATT	6 cut(s)	455, 477, 1007, 1403, 1512, 1594
TatI	WGTACW	1 cut(s)	1062
TauI	GCSGC	2 cut(s)	56, 214
TfiI	GAWTC	3 cut(s)	139, 1072, 1561
Tru1I	TTAA	5 cut(s)	252, 855, 1211, 1316, 1758
Tru9I	TTAA	5 cut(s)	252, 855, 1211, 1316, 1758
TscAI	CASTG	5 cut(s)	699, 742, 829, 1030, 1150
TseFI	GTSAC	3 cut(s)	307, 394, 1997
Tsp45I	GTSAC	3 cut(s)	307, 394, 1997
TspDTI	ATGAA	9 cut(s)	41, 402, 575, 959, 1053, 1362, 1505, 1604, 2123
TspGWI	ACGGA	5 cut(s)	115, 284, 530, 606, 1777
TspRI	CASTG	5 cut(s)	699, 742, 829, 1030, 1150
Vha464I	CTTAAG	1 cut(s)	251
VneI	GTGCAC	3 cut(s)	1204, 1527, 1866
VpaK11BI	GGWCC	5 cut(s)	76, 769, 1703, 1781, 1904
XagI	CCTNNNNNAGG	1 cut(s)	1388
XapI	RAATTY	4 cut(s)	1007, 1403, 1512, 1594
XbaI	TCTAGA	1 cut(s)	1508
XhoI	CTCGAG	2 cut(s)	134, 217
XmiI	GTMKAC	1 cut(s)	8
XspI	CTAG	4 cut(s)	528, 1467, 1509, 1532
Zsp2I	ATGCAT	2 cut(s)	722, 1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.