Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0002068

Physical Location & Seq

Genomic Coordinates Forward (+)

15995595 .. 16001332

Gene Structure 5738 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG104100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2043 bp

ATGGACCCCTCCAACGAGGTCACCTCCGTCATTCTCGACAATCGCGACTTCGTTATGATCCTCACGACCTCGATTGCCGTTTTGATCGGCTGCGTCGTCGTTTTTGTGTGGCGGCGATCGAGCGCTCAGAAGAAGCCCAGGGTCGTCCAGCCTCTTAAGCCGTTGATCATCGCGGAGCCTGAGCCGGAGCTCGACGATGGAAGCAAGAAGGTCACAATCTTCTTCGGCACGCAAACCGGTACTGCTGAGGGATTTGCCAAGGCTTTTGCTGAGGAAGCGAAAGCTCGCTATGAAAATGTCACATTCAAAGTTGTTGATTTGGACGATTATGCAGCTGATGACGAGGCGTATGAGGAGAAATTGAAAAATGAGAGTCTGGCAATTTTCTTTTTGGCGACATATGGTGATGGTGAGCCGACGGATAATGCTGCTAGATTTTACAAGTGGTTTACAGAGGGTAAAGAGAAGGAAGCATGGCTTCAAAATCTTCATTACGGAGTGTTTGGTCTTGGAAACAGGCAGTATGAGCATTTCAACAAGGTGGCCGTGGTTGTGGATGAGTTCCTCGCTGCTCAGGGTGGGAAGCGACTTGTTCCAGTGGGCCTCGGAGATGATGATCAATGCATAGAGGATGATTTCACCGCATGGCGAGAGTTGATGTGGCCTGAGTTGGACCAGTTGCTTCGAGATGAGGATGATGCAACCACACCTGCTTGCACACCTTACACTGCTGCTGTTTTGGAGTATCGAGTTGTATTTCATGATGCTGCTGATGTATCAGTAGAGGATAAGAGCTGGAATAATGCAAATGGTCATGCTGTTATTGATGCTCAGCATCCATGCAGGGCTAATGTTGCTGTGAGAAAGGAACTTCATACTCCTGCATCTGATCGTTCTTGCACCCATCTCGAATTTGATATTGCTGGCACTGGACTTACATACGAAACTGGTGATCATGTCGGTGTGTACTGTGAGAATCTTTCTGAAACTGTGGAAGAGGCGATAAGTTTGTTGGGCTTGGCACCGGAAACATACTTCTCGATGCACACTGATAAGGATGATGGTACACCACTTAGCGGAGGCTCCTTGTTACCTCCCTTTCCACCGTGCACATTAAGAACAGCACTAACTCGATATGCTGATCTTTTGAGTTCTCCGAAAAAGTCTGCCCTGCTGGCTTTAGCTGCCTATGCATGTGATCCAGCTGAAGCGGATCGATTAAGACATCTTGCATCACCTGCTGGGAAGGATGAATATGCTCAGTGGATGGTTGCGAGTCAGAGAAGCCTTCTTGAGGTCATGGCTGAATTTCCATCAGCCAAGCCCCCACTTGGAGTCTTCTTTGCAGCAGTTGCTCCAAGGTTGCAGCCTAGATACTATTCAATCTCGTCATCCCCGAGGATGGCGCCATCTAGAATTCATGTTACTTATGCACTAGTTTATGACAAAACATCAACAGGAAGGATTCACAAAGGAGTGTGTTCAACCTGGATGAAGAATTCTGTGCCTTCCGCAAAAAGTGGAGATTGTAGTTGGGCACCCGTTTTTGTTCGGCAATCAAACTTCAGACTTCCCGTTGATACTAAAGTACCTATTCTTATGATTGGTCCTGGAACTGGATTGGCTCCTTTCAGGGGTTTCCTGCAGGAAAGATTAGCTCTTAAAGAAGATGGAGCAGAACTTGGACCCTCCGTATTATTCTTTGGATGCAGAAATAGTCAAATGGACTACATTTATGAAGATGAGCTGAACAACTTTGTAGAAAAAGATGCACTTTCTGAGCTTGTGGTTGCATTCTCCCGCCAGGGACCCACTAAGGAATATGTGCAACATAAAATGATGCAGAAGGCTTCAGACATCTGGAACATGCTATCTCAGGGAGGTTATATTTACGTTTGTGGTGATGCTAAGGGCATGGCCAGGGATGTCCACCGGACTCTTCACACAATCGTGCAGGAGCAGGGATCTCTGGACAGCTCCAAAGCTGAGAGCATGGTGAAAAATCTGCAAATGAATGGCAGGTATTTGCGTGATGTGTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003958 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006725 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009698 GO:0009987 GO:0012505 GO:0016491 GO:0016651 GO:0016653 GO:0019748 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FAD_binding_1 Flavodoxin_1 NAD_binding_1

Protein Analysis

680

Amino Acids

75.53

Weight (kDa)

5.12

Isoelectric Point (pI)

40.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Flavodoxin_1	PF00258	73 - 216	3.1e-32	Flavodoxin
FAD_binding_1	PF00667	276 - 497	8e-70	FAD binding domain
NAD_binding_1	PF00175	534 - 644	7.6e-17	Oxidoreductase NAD-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002068)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30210 AT4G30210 AT4G30210
fragaria_vesca	FvH4_4g08180
malus_domestica	MD13G1253300.v1.1 MD16G1257600.v1.1
prunus_persica	Prupe.1G085400_v2.0.a1
pyrus_communis	pycom12480g00040 pycom13g22470
rosa_chinensis	RchiOBHm_Chr1g0334331 RchiOBHm_Chr4g0402381 RchiOBHm_Chr4g0402391 RchiOBHm_Chr4g0419591 RchiOBHm_Chr7g0226121
rosa_laevigata	RLG00000009089 RLG00000009093 RLG00000009097 RLG00000009098
rosa_multiflora	Rmu_co8074388.1_g000001 Rmu_sc0002660.1_g000001 Rmu_sc0023635.1_g000001
rosa_roxburghii	Rroxscaffold_1G00022590 Rroxscaffold_5G00347040 Rroxscaffold_5G00347050
rosa_rugosa	Rorug03G0293000 Rorug04G0034500 Rorug04G0034500 Rorug04G0034600 Rorug04G0034700 Rorug04G0034800 Rorug04G0034900 Rorug04G0035000 Rorug05G0246500
rosa_samantha	Rh4AG112500 Rh4AG112600 Rh4BG105000 Rh4BG105100 Rh4CG004400 Rh4CG119300 Rh4CG119400 Rh4DG104000 Rh4DG104100 Rh5AG337100
rosa_wichuraiana	Rw4G009010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	718, 1246
Acc36I	ACCTGC	3 cut(s)	718, 1246, 2010
AccB1I	GGYRCC	3 cut(s)	1021, 1405, 1537
AccII	CGCG	2 cut(s)	45, 173
AciI	CCGC	7 cut(s)	112, 173, 642, 1077, 1211, 1512, 1801
AclWI	GGATC	4 cut(s)	52, 1193, 1221, 1972
AcoI	YGGCCR	2 cut(s)	543, 1917
AcsI	RAATTY	4 cut(s)	911, 1307, 1416, 1498
AcuI	CTGAAG	3 cut(s)	1227, 1549, 1836
AcyI	GRCGYC	1 cut(s)	1406
AfaI	GTAC	4 cut(s)	241, 968, 1066, 1590
AfeI	AGCGCT	1 cut(s)	124
AfiI	CCNNNNNNNGG	6 cut(s)	1076, 1294, 1331, 1402, 1616, 1634
AflII	CTTAAG	1 cut(s)	155
AgeI	ACCGGT	1 cut(s)	236
AgsI	TTSAA	6 cut(s)	307, 364, 482, 535, 1383, 1485
AhlI	ACTAGT	1 cut(s)	1435
AjnI	CCWGG	5 cut(s)	137, 1487, 1609, 1803, 1919
AleI	CACNNNNGTG	1 cut(s)	1949
Alw21I	GWGCWC	2 cut(s)	192, 1112
Alw44I	GTGCAC	1 cut(s)	1108
AlwI	GGATC	4 cut(s)	52, 1193, 1221, 1972
Ama87I	CYCGRG	1 cut(s)	1396
Aor51HI	AGCGCT	1 cut(s)	124
AoxI	GGCC	4 cut(s)	543, 601, 662, 1917
ApaLI	GTGCAC	1 cut(s)	1108
ApeKI	GCWGC	9 cut(s)	90, 332, 428, 569, 731, 767, 1184, 1346, 1366
ApoI	RAATTY	4 cut(s)	911, 1307, 1416, 1498
AsiGI	ACCGGT	1 cut(s)	236
AspLEI	GCGC	2 cut(s)	125, 1408
AspS9I	GGNCC	6 cut(s)	4, 601, 673, 1607, 1685, 1808
AsuHPI	GGTGA	8 cut(s)	13, 416, 422, 631, 962, 1227, 1913, 2008
AvaI	CYCGRG	1 cut(s)	1396
AvaII	GGWCC	5 cut(s)	4, 673, 1607, 1685, 1808
BaeGI	GKGCMC	2 cut(s)	1112, 1540
BaeI	ACNNNNGTAYC	4 cut(s)	1572, 1572, 1605, 1605
BalI	TGGCCA	1 cut(s)	1919
BanI	GGYRCC	3 cut(s)	1021, 1405, 1537
BanII	GRGCYC	1 cut(s)	192
BbsI	GAAGAC	1 cut(s)	1330
Bbv12I	GWGCWC	2 cut(s)	192, 1112
BbvCI	CCTCAGC	2 cut(s)	246, 270
BbvI	GCAGC	9 cut(s)	77, 344, 415, 556, 718, 754, 1171, 1358, 1378
BccI	CCATC	9 cut(s)	191, 401, 912, 1055, 1261, 1321, 1396, 1417, 1664
BceAI	ACGGC	3 cut(s)	62, 145, 530
BcgI	CGANNNNNNTGC	2 cut(s)	889, 923
BciT130I	CCWGG	5 cut(s)	139, 1489, 1611, 1805, 1921
BclI	TGATCA	3 cut(s)	165, 616, 952
BcuI	ACTAGT	1 cut(s)	1435
BfaI	CTAG	4 cut(s)	432, 1371, 1413, 1436
BfmI	CTRYAG	1 cut(s)	1643
BfoI	RGCGCY	2 cut(s)	126, 1409
BfrI	CTTAAG	1 cut(s)	155
BfuAI	ACCTGC	3 cut(s)	718, 1246, 2010
BglI	GCCNNNNNGGC	1 cut(s)	1175
BlpI	GCTNAGC	1 cut(s)	831
Bme1390I	CCNGG	5 cut(s)	139, 1489, 1611, 1805, 1921
Bme18I	GGWCC	5 cut(s)	4, 673, 1607, 1685, 1808
BmeT110I	CYCGRG	1 cut(s)	1396
BmgT120I	GGNCC	6 cut(s)	4, 601, 673, 1607, 1685, 1808
BmrFI	CCNGG	5 cut(s)	139, 1489, 1611, 1805, 1921
BpiI	GAAGAC	1 cut(s)	1330
BplI	GAGNNNNNCTC	2 cut(s)	8, 40
Bpu10I	CCTNAGC	5 cut(s)	180, 246, 270, 573, 1908
Bpu1102I	GCTNAGC	1 cut(s)	831
BpuEI	CTTGAG	1 cut(s)	1313
Bsa29I	ATCGAT	1 cut(s)	1216
BsaBI	GATNNNNATC	1 cut(s)	615
BsaHI	GRCGYC	1 cut(s)	1406
BsaJI	CCNNGG	9 cut(s)	137, 138, 258, 546, 604, 1358, 1397, 1804, 1920
BsaWI	WCCGGW	3 cut(s)	236, 1024, 1932
Bsc4I	CCNNNNNNNGG	6 cut(s)	1076, 1294, 1331, 1402, 1616, 1634
Bse118I	RCCGGY	1 cut(s)	236
Bse1I	ACTGG	5 cut(s)	596, 676, 934, 952, 1621
Bse8I	GATNNNNATC	1 cut(s)	615
BseBI	CCWGG	5 cut(s)	139, 1489, 1611, 1805, 1921
BseCI	ATCGAT	1 cut(s)	1216
BseDI	CCNNGG	9 cut(s)	137, 138, 258, 546, 604, 1358, 1397, 1804, 1920
BseJI	GATNNNNATC	1 cut(s)	615
BseLI	CCNNNNNNNGG	6 cut(s)	1076, 1294, 1331, 1402, 1616, 1634
BseNI	ACTGG	5 cut(s)	596, 676, 934, 952, 1621
BseRI	GAGGAG	1 cut(s)	368
BseSI	GKGCMC	2 cut(s)	1112, 1540
BseXI	GCAGC	9 cut(s)	77, 344, 415, 556, 718, 754, 1171, 1358, 1378
BseYI	CCCAGC	1 cut(s)	1241
BsgI	GTGCAG	1 cut(s)	1973
Bsh1236I	CGCG	2 cut(s)	45, 173
Bsh1285I	CGRYCG	1 cut(s)	119
BshFI	GGCC	4 cut(s)	545, 603, 664, 1919
BshNI	GGYRCC	3 cut(s)	1021, 1405, 1537
BshTI	ACCGGT	1 cut(s)	236
BshVI	ATCGAT	1 cut(s)	1216
BsiEI	CGRYCG	1 cut(s)	119
BsiHKAI	GWGCWC	2 cut(s)	192, 1112
BsiHKCI	CYCGRG	1 cut(s)	1396
BsiSI	CCGG	4 cut(s)	185, 237, 1025, 1933
BslFI	GGGAC	1 cut(s)	1821
BslI	CCNNNNNNNGG	6 cut(s)	1076, 1294, 1331, 1402, 1616, 1634
BsmFI	GGGAC	1 cut(s)	1821
BsmI	GAATGC	1 cut(s)	1793
BsnI	GGCC	4 cut(s)	545, 603, 664, 1919
BsoBI	CYCGRG	1 cut(s)	1396
Bsp1286I	GDGCHC	3 cut(s)	192, 1112, 1540
Bsp1720I	GCTNAGC	1 cut(s)	831
Bsp68I	TCGCGA	1 cut(s)	45
BspACI	CCGC	7 cut(s)	112, 173, 642, 1077, 1211, 1512, 1801
BspANI	GGCC	4 cut(s)	545, 603, 664, 1919
BspDI	ATCGAT	1 cut(s)	1216
BspFNI	CGCG	2 cut(s)	45, 173
BspHI	TCATGA	1 cut(s)	760
BspMAI	CTGCAG	1 cut(s)	1647
BspMI	ACCTGC	3 cut(s)	718, 1246, 2010
BspPI	GGATC	4 cut(s)	52, 1193, 1221, 1972
BspT107I	GGYRCC	3 cut(s)	1021, 1405, 1537
BspTI	CTTAAG	1 cut(s)	155
BsrFI	RCCGGY	1 cut(s)	236
BsrI	ACTGG	5 cut(s)	596, 676, 934, 952, 1621
BssAI	RCCGGY	1 cut(s)	236
BssECI	CCNNGG	9 cut(s)	137, 138, 258, 546, 604, 1358, 1397, 1804, 1920
BssNI	GRCGYC	1 cut(s)	1406
BssT1I	CCWWGG	2 cut(s)	258, 1358
Bst2UI	CCWGG	5 cut(s)	139, 1489, 1611, 1805, 1921
Bst4CI	ACNGT	3 cut(s)	971, 991, 1107
Bst6I	CTCTTC	2 cut(s)	990, 1944
BstACI	GRCGYC	1 cut(s)	1406
BstAFI	CTTAAG	1 cut(s)	155
BstAPI	GCANNNNNTGC	2 cut(s)	1238, 1352
BstC8I	GCNNGC	5 cut(s)	230, 286, 715, 925, 1176
BstDSI	CCRYGG	1 cut(s)	546
BstEII	GGTNACC	1 cut(s)	19
BstENI	CCTNNNNNAGG	1 cut(s)	1292
BstFNI	CGCG	2 cut(s)	45, 173
BstH2I	RGCGCY	2 cut(s)	126, 1409
BstHHI	GCGC	2 cut(s)	125, 1408
BstMCI	CGRYCG	1 cut(s)	119
BstMWI	GCNNNNNNNGC	9 cut(s)	157, 275, 526, 854, 1175, 1184, 1190, 1238, 1352
BstNI	CCWGG	5 cut(s)	139, 1489, 1611, 1805, 1921
BstNSI	RCATGY	2 cut(s)	1197, 1870
BstPI	GGTNACC	1 cut(s)	19
BstSCI	CCNGG	5 cut(s)	137, 1487, 1609, 1803, 1919
BstSFI	CTRYAG	1 cut(s)	1643
BstSLI	GKGCMC	2 cut(s)	1112, 1540
BstUI	CGCG	2 cut(s)	45, 173
BstV1I	GCAGC	9 cut(s)	77, 344, 415, 556, 718, 754, 1171, 1358, 1378
BstV2I	GAAGAC	1 cut(s)	1330
BstX2I	RGATCY	1 cut(s)	1964
BstYI	RGATCY	1 cut(s)	1964
Bsu15I	ATCGAT	1 cut(s)	1216
BsuRI	GGCC	4 cut(s)	545, 603, 664, 1919
BsuTUI	ATCGAT	1 cut(s)	1216
BtgI	CCRYGG	1 cut(s)	546
BtgZI	GCGATG	1 cut(s)	154
BtsI	GCAGTG	1 cut(s)	726
BtsIMutI	CAGTG	5 cut(s)	603, 726, 927, 1047, 1268
BtuMI	TCGCGA	1 cut(s)	45
BveI	ACCTGC	3 cut(s)	718, 1246, 2010
Cac8I	GCNNGC	5 cut(s)	230, 286, 715, 925, 1176
CciI	TCATGA	1 cut(s)	760
CfoI	GCGC	2 cut(s)	125, 1408
Cfr10I	RCCGGY	1 cut(s)	236
Cfr13I	GGNCC	6 cut(s)	4, 601, 673, 1607, 1685, 1808
ClaI	ATCGAT	1 cut(s)	1216
CseI	GACGC	1 cut(s)	82
Csp6I	GTAC	4 cut(s)	240, 967, 1065, 1589
CspAI	ACCGGT	1 cut(s)	236
CviQI	GTAC	4 cut(s)	240, 967, 1065, 1589
DinI	GGCGCC	1 cut(s)	1407
EaeI	YGGCCR	2 cut(s)	543, 1917
Eam1104I	CTCTTC	2 cut(s)	990, 1944
EarI	CTCTTC	2 cut(s)	990, 1944
Ecl136II	GAGCTC	1 cut(s)	190
Eco130I	CCWWGG	2 cut(s)	258, 1358
Eco24I	GRGCYC	1 cut(s)	192
Eco47I	GGWCC	5 cut(s)	4, 673, 1607, 1685, 1808
Eco47III	AGCGCT	1 cut(s)	124
Eco53kI	GAGCTC	1 cut(s)	190
Eco57I	CTGAAG	3 cut(s)	1227, 1549, 1836
Eco88I	CYCGRG	1 cut(s)	1396
Eco91I	GGTNACC	1 cut(s)	19
EcoICRI	GAGCTC	1 cut(s)	190
EcoNI	CCTNNNNNAGG	1 cut(s)	1292
EcoO109I	RGGNCCY	1 cut(s)	1808
EcoO65I	GGTNACC	1 cut(s)	19
EcoRI	GAATTC	2 cut(s)	1416, 1498
EcoRII	CCWGG	5 cut(s)	137, 1487, 1609, 1803, 1919
EcoT14I	CCWWGG	2 cut(s)	258, 1358
EcoT22I	ATGCAT	2 cut(s)	626, 1195
EcoT38I	GRGCYC	1 cut(s)	192
EgeI	GGCGCC	1 cut(s)	1407
EheI	GGCGCC	1 cut(s)	1407
ErhI	CCWWGG	2 cut(s)	258, 1358
FalI	AAGNNNNNCTT	4 cut(s)	462, 494, 1758, 1790
FaqI	GGGAC	1 cut(s)	1821
FauI	CCCGC	1 cut(s)	1808
FauNDI	CATATG	1 cut(s)	400
FbaI	TGATCA	3 cut(s)	165, 616, 952
FriOI	GRGCYC	1 cut(s)	192
FspBI	CTAG	4 cut(s)	432, 1371, 1413, 1436
GlaI	GCGC	2 cut(s)	124, 1407
GsaI	CCCAGC	1 cut(s)	1245
HaeII	RGCGCY	2 cut(s)	126, 1409
HaeIII	GGCC	4 cut(s)	545, 603, 664, 1919
HapII	CCGG	4 cut(s)	185, 237, 1025, 1933
HgaI	GACGC	1 cut(s)	82
HhaI	GCGC	2 cut(s)	125, 1408
Hin1I	GRCGYC	1 cut(s)	1406
Hin6I	GCGC	2 cut(s)	123, 1406
HinP1I	GCGC	2 cut(s)	123, 1406
HinfI	GANTC	6 cut(s)	373, 976, 1276, 1335, 1465, 1936
HpaII	CCGG	4 cut(s)	185, 237, 1025, 1933
HphI	GGTGA	8 cut(s)	13, 416, 422, 631, 962, 1227, 1913, 2008
Hpy166II	GTNNAC	5 cut(s)	450, 967, 1067, 1110, 1930
Hpy188I	TCNGA	9 cut(s)	129, 608, 889, 985, 1158, 1281, 1568, 1780, 1855
Hpy8I	GTNNAC	5 cut(s)	450, 967, 1067, 1110, 1930
Hpy99I	CGWCG	4 cut(s)	98, 101, 197, 421
HpyAV	CCTTC	7 cut(s)	202, 460, 1240, 1298, 1455, 1518, 1840
HpyCH4III	ACNGT	3 cut(s)	971, 991, 1107
HpyCH4IV	ACGT	1 cut(s)	1893
HpyF10VI	GCNNNNNNNGC	9 cut(s)	157, 275, 526, 854, 1175, 1184, 1190, 1238, 1352
HpySE526I	ACGT	1 cut(s)	1893
Hsp92I	GRCGYC	1 cut(s)	1406
HspAI	GCGC	2 cut(s)	123, 1406
KasI	GGCGCC	1 cut(s)	1405
KflI	GGGWCCC	1 cut(s)	1808
Ksp22I	TGATCA	3 cut(s)	165, 616, 952
LmnI	GCTCC	8 cut(s)	175, 187, 1088, 1360, 1630, 1673, 1957, 1982
Lsp1109I	GCAGC	9 cut(s)	77, 344, 415, 556, 718, 754, 1171, 1358, 1378
MaeI	CTAG	4 cut(s)	432, 1371, 1413, 1436
MaeII	ACGT	1 cut(s)	1893
MaeIII	GTNAC	5 cut(s)	19, 211, 298, 1089, 1423
MflI	RGATCY	1 cut(s)	1964
MhlI	GDGCHC	3 cut(s)	192, 1112, 1540
MlsI	TGGCCA	1 cut(s)	1919
MluCI	AATT	6 cut(s)	359, 381, 911, 1307, 1416, 1498
MluNI	TGGCCA	1 cut(s)	1919
Mly113I	GGCGCC	1 cut(s)	1406
MlyI	GAGTC	4 cut(s)	382, 1285, 1344, 1930
MmeI	TCCRAC	2 cut(s)	36, 651
Mox20I	TGGCCA	1 cut(s)	1919
Mph1103I	ATGCAT	2 cut(s)	626, 1195
MscI	TGGCCA	1 cut(s)	1919
MseI	TTAA	4 cut(s)	156, 1115, 1220, 1662
MslI	CAYNNNNRTG	2 cut(s)	960, 1949
Msp20I	TGGCCA	1 cut(s)	1919
MspA1I	CMGCKG	2 cut(s)	335, 1205
MspCI	CTTAAG	1 cut(s)	155
MspI	CCGG	4 cut(s)	185, 237, 1025, 1933
MspR9I	CCNGG	5 cut(s)	139, 1489, 1611, 1805, 1921
Mva1269I	GAATGC	1 cut(s)	1793
MvaI	CCWGG	5 cut(s)	139, 1489, 1611, 1805, 1921
MvnI	CGCG	2 cut(s)	45, 173
MwoI	GCNNNNNNNGC	9 cut(s)	157, 275, 526, 854, 1175, 1184, 1190, 1238, 1352
NarI	GGCGCC	1 cut(s)	1406
NdeI	CATATG	1 cut(s)	400
NmuCI	GTSAC	3 cut(s)	19, 211, 298
NruI	TCGCGA	1 cut(s)	45
NsiI	ATGCAT	2 cut(s)	626, 1195
NspI	RCATGY	2 cut(s)	1197, 1870
OliI	CACNNNNGTG	1 cut(s)	1949
PagI	TCATGA	1 cut(s)	760
PaqCI	CACCTGC	2 cut(s)	718, 1246
PasI	CCCWGGG	1 cut(s)	138
PcsI	WCGNNNNNNNCGW	3 cut(s)	42, 93, 1394
PctI	GAATGC	1 cut(s)	1793
PfeI	GAWTC	2 cut(s)	976, 1465
PfoI	TCCNGGA	1 cut(s)	1609
PinAI	ACCGGT	1 cut(s)	236
Ple19I	CGATCG	1 cut(s)	119
PleI	GAGTC	4 cut(s)	381, 1284, 1343, 1930
PluTI	GGCGCC	1 cut(s)	1409
PpsI	GAGTC	4 cut(s)	381, 1284, 1343, 1930
PpuMI	RGGWCCY	1 cut(s)	1808
Psp124BI	GAGCTC	1 cut(s)	192
Psp5II	RGGWCCY	1 cut(s)	1808
Psp6I	CCWGG	5 cut(s)	137, 1487, 1609, 1803, 1919
PspEI	GGTNACC	1 cut(s)	19
PspFI	CCCAGC	1 cut(s)	1241
PspGI	CCWGG	5 cut(s)	137, 1487, 1609, 1803, 1919
PspPI	GGNCC	6 cut(s)	4, 601, 673, 1607, 1685, 1808
PspPPI	RGGWCCY	1 cut(s)	1808
PstI	CTGCAG	1 cut(s)	1647
PsuI	RGATCY	1 cut(s)	1964
PvuI	CGATCG	1 cut(s)	119
PvuII	CAGCTG	2 cut(s)	335, 1205
RruI	TCGCGA	1 cut(s)	45
RsaI	GTAC	4 cut(s)	241, 968, 1066, 1590
RsaNI	GTAC	4 cut(s)	240, 967, 1065, 1589
RseI	CAYNNNNRTG	2 cut(s)	960, 1949
SacI	GAGCTC	1 cut(s)	192
SaqAI	TTAA	4 cut(s)	156, 1115, 1220, 1662
Sau96I	GGNCC	6 cut(s)	4, 601, 673, 1607, 1685, 1808
SbfI	CCTGCAGG	1 cut(s)	1647
SchI	GAGTC	4 cut(s)	382, 1285, 1344, 1930
ScrFI	CCNGG	5 cut(s)	139, 1489, 1611, 1805, 1921
SdaI	CCTGCAGG	1 cut(s)	1647
SduI	GDGCHC	3 cut(s)	192, 1112, 1540
SfcI	CTRYAG	1 cut(s)	1643
SfoI	GGCGCC	1 cut(s)	1407
SinI	GGWCC	5 cut(s)	4, 673, 1607, 1685, 1808
SmiMI	CAYNNNNRTG	2 cut(s)	960, 1949
SmlI	CTYRAG	2 cut(s)	155, 1292
SmoI	CTYRAG	2 cut(s)	155, 1292
SpeI	ACTAGT	1 cut(s)	1435
Sse8387I	CCTGCAGG	1 cut(s)	1647
Sse9I	AATT	6 cut(s)	359, 381, 911, 1307, 1416, 1498
SsiI	CCGC	7 cut(s)	112, 173, 642, 1077, 1211, 1512, 1801
SspDI	GGCGCC	1 cut(s)	1405
SspMI	CTAG	4 cut(s)	432, 1371, 1413, 1436
SstI	GAGCTC	1 cut(s)	192
StyD4I	CCNGG	5 cut(s)	137, 1487, 1609, 1803, 1919
StyI	CCWWGG	2 cut(s)	258, 1358
TaaI	ACNGT	3 cut(s)	971, 991, 1107
TaiI	ACGT	1 cut(s)	1896
TasI	AATT	6 cut(s)	359, 381, 911, 1307, 1416, 1498
TatI	WGTACW	1 cut(s)	966
TauI	GCSGC	1 cut(s)	115
TfiI	GAWTC	2 cut(s)	976, 1465
Tru1I	TTAA	4 cut(s)	156, 1115, 1220, 1662
Tru9I	TTAA	4 cut(s)	156, 1115, 1220, 1662
TscAI	CASTG	5 cut(s)	603, 733, 934, 1054, 1268
TseFI	GTSAC	3 cut(s)	19, 211, 298
TseI	GCWGC	9 cut(s)	90, 332, 428, 569, 731, 767, 1184, 1346, 1366
Tsp45I	GTSAC	3 cut(s)	19, 211, 298
TspDTI	ATGAA	9 cut(s)	306, 479, 749, 863, 1266, 1409, 1508, 1752, 2027
TspGWI	ACGGA	4 cut(s)	16, 434, 510, 1681
TspRI	CASTG	5 cut(s)	603, 733, 934, 1054, 1268
Vha464I	CTTAAG	1 cut(s)	155
VneI	GTGCAC	1 cut(s)	1108
VpaK11BI	GGWCC	5 cut(s)	4, 673, 1607, 1685, 1808
XagI	CCTNNNNNAGG	1 cut(s)	1292
XapI	RAATTY	4 cut(s)	911, 1307, 1416, 1498
XbaI	TCTAGA	1 cut(s)	1412
XceI	RCATGY	2 cut(s)	1197, 1870
XspI	CTAG	4 cut(s)	432, 1371, 1413, 1436
Zsp2I	ATGCAT	2 cut(s)	626, 1195

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.