pycom12g00550

Hsp20/alpha crystallin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012033
Physical Location & Seq
Forward (+)
506549 .. 507431
883 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGAAACCAAACCTGCAGCACAGCATGAACAGCGTTATGATGATTTTAAACCGCGTTGCCAATGGAAGCAGGAAGGACCTAAAATTCTTGAAGTTCGTCTACCAGGTTTCAGAAGACAAGATATTAGAATTCAAAACTGCACGAAGTTGGGCACTCTAACCATCAGCGGAGAGCAACCTAGGGTTGAAGAAACTAAATCTGCTGCTTTACCCAGACGCTTCAGGAAAGAGATCAAAATTTCTAAAAATTTTAATACGGACGGAATCGGGGCTAAGTTTTCCGGTGAAATTCTTTCCATAACTATTCCAAAGGAAGCTCAGCTTTCTGCATGGTCGTCGGTTAGTGACTACCTAGTTTGTTTAAGAAGCAAAGTTTCGAGGCCAAGACTGAGCAAGAAGACGGCTGTGACGGCGGTGGTGATTGTTTTGGTCGTGGCTCTGGGAGTTTGTGCAGTATCTAGATTTCCAAAGTCAACTTGCTCCCTGATGCCTTTGATCAAGTTCCTTGAATGCCTTTGTGGCAATACTAGCAAGTACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

181

Amino Acids

20.26

Weight (kDa)

9.89

Isoelectric Point (pI)

38.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 30 - 107 8.3e-07 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54400
fragaria_vesca FvH4_7g04830
malus_domestica MD12G1007500.v1.1 MD12G1007600.v1.1 MD14G1004900.v1.1
prunus_persica Prupe.7G008300_v2.0.a1
pyrus_communis pycom12g00550 pycom12g00560 pycom14g00330
rosa_chinensis RchiOBHm_Chr3g0496561
rosa_laevigata RLG00000022518
rosa_multiflora Rmu_sc0004122.1_g000006
rosa_roxburghii Rroxscaffold_6G00388910
rosa_rugosa Rorug03G0284600
rosa_samantha Rh3AG329800 Rh3BG365500 Rh3CG362800 Rh3DG365800
rosa_wichuraiana Rw3G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 100
AccII CGCG 1 cut(s) 55
AciI CCGC 3 cut(s) 53, 168, 413
AcsI RAATTY 5 cut(s) 84, 129, 237, 247, 288
AcuI CTGAAG 1 cut(s) 205
AfaI GTAC 1 cut(s) 536
AgsI TTSAA 4 cut(s) 92, 134, 188, 509
AjnI CCWGG 1 cut(s) 103
AluBI AGCT 2 cut(s) 317, 322
AluI AGCT 2 cut(s) 317, 322
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 17, 203
ApoI RAATTY 5 cut(s) 84, 129, 237, 247, 288
AspA2I CCTAGG 1 cut(s) 179
AspS9I GGNCC 1 cut(s) 77
AsuHPI GGTGA 2 cut(s) 296, 430
AvaII GGWCC 1 cut(s) 77
AvrII CCTAGG 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 155
BarI GAAGNNNNNNTAC 2 cut(s) 84, 116
BbsI GAAGAC 2 cut(s) 121, 404
BbvI GCAGC 2 cut(s) 29, 190
BccI CCATC 1 cut(s) 170
BceAI ACGGC 2 cut(s) 417, 426
BcgI CGANNNNNNTGC 2 cut(s) 318, 352
BciT130I CCWGG 1 cut(s) 105
BclI TGATCA 1 cut(s) 495
BfaI CTAG 4 cut(s) 180, 353, 459, 528
BfmI CTRYAG 1 cut(s) 15
BfuAI ACCTGC 1 cut(s) 22
BglI GCCNNNNNGGC 1 cut(s) 519
BisI GCNGC 2 cut(s) 18, 204
BlnI CCTAGG 1 cut(s) 179
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 2 cut(s) 19, 205
Bme1390I CCNGG 1 cut(s) 105
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 477
BpiI GAAGAC 2 cut(s) 121, 404
Bpu1102I GCTNAGC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 179
BsaWI WCCGGW 1 cut(s) 281
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 179
BseMII CTCAG 2 cut(s) 332, 380
BseSI GKGCMC 1 cut(s) 155
BseXI GCAGC 2 cut(s) 29, 190
BsgI GTGCAG 2 cut(s) 124, 471
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 1 cut(s) 382
BsiSI CCGG 1 cut(s) 282
BsmI GAATGC 1 cut(s) 515
BsnI GGCC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 155
Bsp143I GATC 2 cut(s) 231, 495
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 3 cut(s) 53, 168, 413
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 2 cut(s) 331, 381
BspFNI CGCG 1 cut(s) 55
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 22
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 231, 495
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 539
BstDEI CTNAG 3 cut(s) 273, 318, 389
BstFNI CGCG 1 cut(s) 55
BstKTI GATC 2 cut(s) 234, 498
BstMBI GATC 2 cut(s) 231, 495
BstMWI GCNNNNNNNGC 4 cut(s) 31, 410, 519, 528
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 155
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 29, 190
BstV2I GAAGAC 2 cut(s) 121, 404
BsuRI GGCC 1 cut(s) 382
BveI ACCTGC 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 77
CseI GACGC 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 103
Csp6I GTAC 1 cut(s) 535
CviAII CATG 2 cut(s) 26, 330
CviJI RGCY 6 cut(s) 272, 317, 322, 382, 404, 437
CviKI_1 RGCY 6 cut(s) 272, 317, 322, 382, 404, 437
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 3 cut(s) 273, 318, 389
DpnI GATC 2 cut(s) 233, 497
DpnII GATC 2 cut(s) 231, 495
DraI TTTAAA 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 77
Eco57I CTGAAG 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 2 cut(s) 29, 333
FaiI YATR 4 cut(s) 27, 39, 299, 331
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FatI CATG 2 cut(s) 25, 329
FbaI TGATCA 1 cut(s) 495
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 2 cut(s) 18, 204
Fsp4HI GCNGC 2 cut(s) 18, 204
FspBI CTAG 4 cut(s) 180, 353, 459, 528
GluI GCNGC 2 cut(s) 18, 204
HaeIII GGCC 1 cut(s) 382
HapII CCGG 1 cut(s) 282
HgaI GACGC 1 cut(s) 225
Hin1II CATG 2 cut(s) 29, 333
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 1 cut(s) 264
HpaII CCGG 1 cut(s) 282
HphI GGTGA 2 cut(s) 296, 430
Hpy166II GTNNAC 2 cut(s) 101, 474
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 3 cut(s) 89, 223, 459
Hpy8I GTNNAC 2 cut(s) 101, 474
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 68
HpyCH4III ACNGT 1 cut(s) 539
HpyCH4V TGCA 4 cut(s) 17, 141, 329, 452
HpyF10VI GCNNNNNNNGC 4 cut(s) 31, 410, 519, 528
HpyF3I CTNAG 3 cut(s) 273, 318, 389
Hsp92II CATG 2 cut(s) 29, 333
Ksp22I TGATCA 1 cut(s) 495
Kzo9I GATC 2 cut(s) 231, 495
LmnI GCTCC 1 cut(s) 485
LpnPI CCDG 9 cut(s) 27, 56, 90, 117, 208, 226, 295, 425, 497
Lsp1109I GCAGC 2 cut(s) 29, 190
LweI GCATC 1 cut(s) 477
MabI ACCWGGT 1 cut(s) 103
MaeI CTAG 4 cut(s) 180, 353, 459, 528
MaeIII GTNAC 2 cut(s) 344, 406
MalI GATC 2 cut(s) 233, 497
MboI GATC 2 cut(s) 231, 495
MboII GAAGA 3 cut(s) 126, 200, 409
MhlI GDGCHC 1 cut(s) 155
MluCI AATT 5 cut(s) 84, 129, 237, 247, 288
MnlI CCTC 1 cut(s) 372
MseI TTAA 3 cut(s) 48, 252, 362
MspA1I CMGCKG 1 cut(s) 168
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 1 cut(s) 105
Mva1269I GAATGC 1 cut(s) 515
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 4 cut(s) 31, 410, 519, 528
NdeII GATC 2 cut(s) 231, 495
NlaIII CATG 2 cut(s) 29, 333
NmuCI GTSAC 2 cut(s) 344, 406
PctI GAATGC 1 cut(s) 515
PfeI GAWTC 1 cut(s) 264
PkrI GCNGC 2 cut(s) 19, 205
PpuMI RGGWCCY 1 cut(s) 77
Psp5II RGGWCCY 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspPI GGNCC 1 cut(s) 77
PspPPI RGGWCCY 1 cut(s) 77
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
SaqAI TTAA 3 cut(s) 48, 252, 362
SatI GCNGC 2 cut(s) 18, 204
Sau3AI GATC 2 cut(s) 231, 495
Sau96I GGNCC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 155
SetI ASST 7 cut(s) 16, 82, 109, 181, 319, 324, 354
SexAI ACCWGGT 1 cut(s) 103
SfaNI GCATC 1 cut(s) 477
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 1 cut(s) 77
Sse9I AATT 5 cut(s) 84, 129, 237, 247, 288
SsiI CCGC 3 cut(s) 53, 168, 413
SspMI CTAG 4 cut(s) 180, 353, 459, 528
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 539
TaqI TCGA 1 cut(s) 377
TasI AATT 5 cut(s) 84, 129, 237, 247, 288
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 3 cut(s) 48, 252, 362
Tru9I TTAA 3 cut(s) 48, 252, 362
TseFI GTSAC 2 cut(s) 344, 406
TseI GCWGC 2 cut(s) 17, 203
Tsp45I GTSAC 2 cut(s) 344, 406
TspDTI ATGAA 1 cut(s) 42
TspGWI ACGGA 2 cut(s) 272, 276
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 5 cut(s) 84, 129, 237, 247, 288
XbaI TCTAGA 1 cut(s) 458
XmaJI CCTAGG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 100
XspI CTAG 4 cut(s) 180, 353, 459, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.