pycom12g00860

Splicing factor 3B subunit 10 (SF3b10)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007702
Physical Location & Seq
Reverse (-)
732213 .. 733916
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 264 bp
ATGCAGGCCAGTGACAGGTTCAACATCAATTCCCAGCTTGAACACCTTCAAGCTAAGTACGTTGGAACCGGGCACGCCGACTTGAATCGATATGAATGGGCGGTGAATATTCAGCGTGATAGCTACGCTTCATACGTTGGCCATTATCCGATTCTCGCATACTTTGCTCTTGCCGAGAATGAGTCCATTGGGAGAGAACGCTACAACTTTATGCAGAAAATGCTTTTGCCTTGTGGTCCTCCCCCAGAACGAGAAGACGATTGA

Protein Analysis

88

Amino Acids

10.11

Weight (kDa)

5.23

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SF3b10 PF07189 5 - 82 1.7e-37 Splicing factor 3B subunit 10 (SF3b10)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 101
AcoI YGGCCR 1 cut(s) 139
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 1 cut(s) 15
AgsI TTSAA 4 cut(s) 22, 41, 50, 85
AluBI AGCT 3 cut(s) 37, 53, 123
AluI AGCT 3 cut(s) 37, 53, 123
AoxI GGCC 2 cut(s) 6, 139
Asp700I GAANNNNTTC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 236
AsuC2I CCSGG 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 115
AvaII GGWCC 1 cut(s) 236
BaeGI GKGCMC 1 cut(s) 75
BalI TGGCCA 1 cut(s) 141
BcnI CCSGG 1 cut(s) 70
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 236
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 1 cut(s) 70
BpuMI CCSGG 1 cut(s) 70
Bsa29I ATCGAT 1 cut(s) 88
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse1I ACTGG 1 cut(s) 9
BseCI ATCGAT 1 cut(s) 88
BseLI CCNNNNNNNGG 1 cut(s) 15
BseNI ACTGG 1 cut(s) 9
BseSI GKGCMC 1 cut(s) 75
BseYI CCCAGC 1 cut(s) 33
BshFI GGCC 2 cut(s) 8, 141
BshVI ATCGAT 1 cut(s) 88
BsiSI CCGG 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 15
BsnI GGCC 2 cut(s) 8, 141
Bsp1286I GDGCHC 1 cut(s) 75
BspACI CCGC 1 cut(s) 101
BspANI GGCC 2 cut(s) 8, 141
BspDI ATCGAT 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 67
BsrI ACTGG 1 cut(s) 9
BstAPI GCANNNNNTGC 2 cut(s) 164, 220
BstC8I GCNNGC 2 cut(s) 6, 75
BstDEI CTNAG 1 cut(s) 54
BstMWI GCNNNNNNNGC 2 cut(s) 164, 220
BstSCI CCNGG 1 cut(s) 68
BstSLI GKGCMC 1 cut(s) 75
Bsu15I ATCGAT 1 cut(s) 88
BsuRI GGCC 2 cut(s) 8, 141
BsuTUI ATCGAT 1 cut(s) 88
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 2 cut(s) 6, 75
Cfr13I GGNCC 1 cut(s) 236
ClaI ATCGAT 1 cut(s) 88
Csp6I GTAC 1 cut(s) 58
CviJI RGCY 5 cut(s) 8, 37, 53, 123, 141
CviKI_1 RGCY 5 cut(s) 8, 37, 53, 123, 141
CviQI GTAC 1 cut(s) 58
DdeI CTNAG 1 cut(s) 54
EaeI YGGCCR 1 cut(s) 139
Eco47I GGWCC 1 cut(s) 236
FaiI YATR 4 cut(s) 93, 133, 160, 212
GsaI CCCAGC 1 cut(s) 37
HaeIII GGCC 2 cut(s) 8, 141
HapII CCGG 1 cut(s) 69
HinfI GANTC 3 cut(s) 85, 151, 182
HpaII CCGG 1 cut(s) 69
HphI GGTGA 1 cut(s) 115
Hpy188I TCNGA 1 cut(s) 150
HpyAV CCTTC 1 cut(s) 56
HpyCH4IV ACGT 2 cut(s) 60, 135
HpyCH4V TGCA 2 cut(s) 4, 214
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 220
HpyF3I CTNAG 1 cut(s) 54
HpySE526I ACGT 2 cut(s) 60, 135
LpnPI CCDG 4 cut(s) 22, 47, 82, 258
MaeII ACGT 2 cut(s) 60, 135
MaeIII GTNAC 1 cut(s) 11
MhlI GDGCHC 1 cut(s) 75
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 1 cut(s) 28
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 43
MnlI CCTC 1 cut(s) 249
Mox20I TGGCCA 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 45
MscI TGGCCA 1 cut(s) 141
Msp20I TGGCCA 1 cut(s) 141
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 70
MwoI GCNNNNNNNGC 2 cut(s) 164, 220
NciI CCSGG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 67
NmeAIII GCCGAG 1 cut(s) 199
NmuCI GTSAC 1 cut(s) 11
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 2 cut(s) 85, 151
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 1 cut(s) 236
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
Sau96I GGNCC 1 cut(s) 236
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 70
SduI GDGCHC 1 cut(s) 75
SetI ASST 7 cut(s) 20, 39, 48, 55, 63, 125, 138
SinI GGWCC 1 cut(s) 236
Sse9I AATT 1 cut(s) 28
SsiI CCGC 1 cut(s) 101
SspI AATATT 1 cut(s) 109
StyD4I CCNGG 1 cut(s) 68
TaiI ACGT 2 cut(s) 63, 138
TaqI TCGA 1 cut(s) 88
TasI AATT 1 cut(s) 28
TfiI GAWTC 2 cut(s) 85, 151
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 2 cut(s) 108, 120
TspRI CASTG 1 cut(s) 16
VpaK11BI GGWCC 1 cut(s) 236
XmnI GAANNNNTTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.