pycom12g04530

strigolactone esterase D14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013729
Physical Location & Seq
Forward (+)
4397469 .. 4397924
456 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g04530.1

Sequence Viewer

Length: 456 bp
ATGGAAGCCATGTGCCATGGAGGAGGCATTGTCCAAGCCCTAAACACTCATGTGTACGGAAATGAAACTCAGACTTTGGTTCTTGCTCATGGGTTTGGTGCAGACCAGACAATTTGGCACTTCCTCATCCCGTTTCTTGCATGCTACTTCAAGGTTGTGGTTTTTGACTCGGTTTACTCTCCAAATGTTGATCCCAAACTCTATGATCCCAAAAGGTACTCGAACTTCAGTGCTTACGCAGAAGACCTGCTCTGCCTTCTTGATCATCTCAATGTTAAAAGGACTGTATATTTGGGCTCCATGGCTGCCATGGTTGGATGTATCGCTTCTGTTCAAAGACCACAACTCTTCCAGCACCTTATCCTACTAAGTGGCTCTCCCAGGTCATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

152

Amino Acids

16.59

Weight (kDa)

6.63

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 25 - 127 2.3e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 255
AclWI GGATC 2 cut(s) 185, 200
AcuI CTGAAG 1 cut(s) 211
AfaI GTAC 2 cut(s) 56, 218
AgsI TTSAA 2 cut(s) 151, 335
AjnI CCWGG 1 cut(s) 380
AleI CACNNNNGTG 1 cut(s) 50
AlwI GGATC 2 cut(s) 185, 200
ApeKI GCWGC 1 cut(s) 305
ArsI GACNNNNNNTTYG 2 cut(s) 274, 306
BanII GRGCYC 1 cut(s) 299
BarI GAAGNNNNNNTAC 2 cut(s) 209, 241
BbsI GAAGAC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 292
BciT130I CCWGG 1 cut(s) 382
BclI TGATCA 1 cut(s) 262
BfuAI ACCTGC 1 cut(s) 255
BisI GCNGC 1 cut(s) 306
BlsI GCNGC 1 cut(s) 307
Bme1390I CCNGG 1 cut(s) 382
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 249
BsaJI CCNNGG 4 cut(s) 16, 300, 309, 380
BsaXI ACNNNNNCTCC 2 cut(s) 15, 45
BseBI CCWGG 1 cut(s) 382
BseDI CCNNGG 4 cut(s) 16, 300, 309, 380
BseGI GGATG 2 cut(s) 126, 323
BseMII CTCAG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 36
BseXI GCAGC 1 cut(s) 292
BsgI GTGCAG 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 3 cut(s) 190, 205, 262
Bsp19I CCATGG 3 cut(s) 16, 300, 309
BspCNI CTCAG 1 cut(s) 82
BspLI GGNNCC 1 cut(s) 298
BspMI ACCTGC 1 cut(s) 255
BspPI GGATC 2 cut(s) 185, 200
BssECI CCNNGG 4 cut(s) 16, 300, 309, 380
BssMI GATC 3 cut(s) 190, 205, 262
BssT1I CCWWGG 3 cut(s) 16, 300, 309
Bst2UI CCWGG 1 cut(s) 382
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 2 cut(s) 69, 368
BstDSI CCRYGG 3 cut(s) 16, 300, 309
BstF5I GGATG 2 cut(s) 126, 323
BstKTI GATC 3 cut(s) 193, 208, 265
BstMBI GATC 3 cut(s) 190, 205, 262
BstNI CCWGG 1 cut(s) 382
BstNSI RCATGY 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 380
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 249
BtgI CCRYGG 3 cut(s) 16, 300, 309
BtsCI GGATG 2 cut(s) 126, 323
BtsIMutI CAGTG 1 cut(s) 235
BveI ACCTGC 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 55, 217
CviAII CATG 7 cut(s) 10, 17, 50, 89, 141, 301, 310
CviJI RGCY 5 cut(s) 8, 38, 297, 305, 375
CviKI_1 RGCY 5 cut(s) 8, 38, 297, 305, 375
CviQI GTAC 2 cut(s) 55, 217
DdeI CTNAG 2 cut(s) 69, 368
DpnI GATC 3 cut(s) 192, 207, 264
DpnII GATC 3 cut(s) 190, 205, 262
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco130I CCWWGG 3 cut(s) 16, 300, 309
Eco24I GRGCYC 1 cut(s) 299
Eco57I CTGAAG 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 380
EcoT14I CCWWGG 3 cut(s) 16, 300, 309
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 3 cut(s) 16, 300, 309
FaeI CATG 7 cut(s) 13, 20, 53, 92, 144, 304, 313
FatI CATG 7 cut(s) 9, 16, 49, 88, 140, 300, 309
FbaI TGATCA 1 cut(s) 262
Fnu4HI GCNGC 1 cut(s) 306
FokI GGATG 2 cut(s) 113, 330
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 1 cut(s) 306
GluI GCNGC 1 cut(s) 306
Hin1II CATG 7 cut(s) 13, 20, 53, 92, 144, 304, 313
HinfI GANTC 1 cut(s) 167
Hpy166II GTNNAC 2 cut(s) 55, 175
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 1 cut(s) 260
Hpy8I GTNNAC 2 cut(s) 55, 175
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 2 cut(s) 101, 140
HpyF3I CTNAG 2 cut(s) 69, 368
Hsp92II CATG 7 cut(s) 13, 20, 53, 92, 144, 304, 313
Ksp22I TGATCA 1 cut(s) 262
Kzo9I GATC 3 cut(s) 190, 205, 262
LmnI GCTCC 1 cut(s) 302
LpnPI CCDG 5 cut(s) 119, 260, 365, 367, 394
Lsp1109I GCAGC 1 cut(s) 292
MalI GATC 3 cut(s) 192, 207, 264
MboI GATC 3 cut(s) 190, 205, 262
MboII GAAGA 2 cut(s) 254, 340
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 1 cut(s) 111
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 3 cut(s) 14, 17, 134
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 2 cut(s) 50, 270
MspR9I CCNGG 1 cut(s) 382
MvaI CCWGG 1 cut(s) 382
NcoI CCATGG 3 cut(s) 16, 300, 309
NdeII GATC 3 cut(s) 190, 205, 262
NlaIII CATG 7 cut(s) 13, 20, 53, 92, 144, 304, 313
NlaIV GGNNCC 1 cut(s) 298
NspI RCATGY 1 cut(s) 144
OliI CACNNNNGTG 1 cut(s) 50
PaeI GCATGC 1 cut(s) 144
PkrI GCNGC 1 cut(s) 307
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
Psp6I CCWGG 1 cut(s) 380
PspGI CCWGG 1 cut(s) 380
PspN4I GGNNCC 1 cut(s) 298
RsaI GTAC 2 cut(s) 56, 218
RsaNI GTAC 2 cut(s) 55, 217
RseI CAYNNNNRTG 2 cut(s) 50, 270
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 1 cut(s) 306
Sau3AI GATC 3 cut(s) 190, 205, 262
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 382
SduI GDGCHC 1 cut(s) 299
SetI ASST 5 cut(s) 156, 218, 249, 360, 386
SmiMI CAYNNNNRTG 2 cut(s) 50, 270
SphI GCATGC 1 cut(s) 144
Sse9I AATT 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 380
StyI CCWWGG 3 cut(s) 16, 300, 309
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 1 cut(s) 221
TasI AATT 1 cut(s) 111
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 1 cut(s) 235
TseI GCWGC 1 cut(s) 305
TspDTI ATGAA 1 cut(s) 78
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 1 cut(s) 235
XceI RCATGY 1 cut(s) 144
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.