Rw3G019680

strigolactone esterase D14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013729
Physical Location & Seq
Forward (+)
22755368 .. 22757452
2085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G019680.1

Sequence Viewer

Length: 438 bp
ATGAAGTACCTCAACACCACAGGATACACCGGAGGCTTTACCAGACCAGAGCTCGATGCAATCTTCAACCAAATCGATACAAATTTCTCAAGCTGGATACCAAATTTTGCTCCACTAGCAATAGGTGCAAACAACACAACTGCAATAGTGGAGTTAGAACGCAGTTTCCATAGAATGAAACCCAAGATTGCACTCAGTGCTGCTGAAAATGTTTTGCTAAGTGACCTCAGAAAAGTTTTGCCGAAAGTAATGGTGCCAACTAGCATAATCCAATCAAAGAAAGACATGGTGGTTCCAGAATCAGTTGCATTTTACATGAAAAGAATGCTTGGCGCTCCAGCAGAAGTTACAATCCTAAACACACAAGGACATTTCCCTCAGTTGACTGTCTATCCTTTACTCTTAAAGGCTCTCAAAGGAGTTCTCCACATCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

145

Amino Acids

16.11

Weight (kDa)

9.7

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 253
AcsI RAATTY 2 cut(s) 82, 103
AdeI CACNNNGTG 1 cut(s) 197
AfaI GTAC 1 cut(s) 8
AgsI TTSAA 1 cut(s) 67
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 2 cut(s) 52, 93
AluI AGCT 2 cut(s) 52, 93
Alw21I GWGCWC 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 2 cut(s) 82, 103
AspLEI GCGC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 253
BanII GRGCYC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 54
BbvI GCAGC 1 cut(s) 187
BciVI GTATCC 2 cut(s) 17, 90
BfaI CTAG 2 cut(s) 116, 261
BfoI RGCGCY 1 cut(s) 336
BfuI GTATCC 2 cut(s) 17, 90
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 255, 294
BmsI GCATC 1 cut(s) 46
BpmI CTGGAG 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 73
Bsa29I ATCGAT 1 cut(s) 75
BsaWI WCCGGW 1 cut(s) 29
BseCI ATCGAT 1 cut(s) 75
BseMII CTCAG 3 cut(s) 208, 241, 392
BseXI GCAGC 1 cut(s) 187
BshNI GGYRCC 1 cut(s) 253
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 54
BsiSI CCGG 1 cut(s) 30
BsmI GAATGC 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 54
BspCNI CTCAG 3 cut(s) 207, 240, 391
BspDI ATCGAT 1 cut(s) 75
BspLI GGNNCC 2 cut(s) 255, 294
BspT107I GGYRCC 1 cut(s) 253
Bst4CI ACNGT 1 cut(s) 388
BstAPI GCANNNNNTGC 2 cut(s) 125, 197
BstDEI CTNAG 4 cut(s) 194, 218, 227, 378
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 1 cut(s) 335
BstMWI GCNNNNNNNGC 3 cut(s) 116, 125, 197
BstV1I GCAGC 1 cut(s) 187
Bsu15I ATCGAT 1 cut(s) 75
BsuI GTATCC 2 cut(s) 17, 90
BsuTUI ATCGAT 1 cut(s) 75
BtsIMutI CAGTG 1 cut(s) 202
CfoI GCGC 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 75
Csp6I GTAC 1 cut(s) 7
CviAII CATG 2 cut(s) 286, 316
CviJI RGCY 4 cut(s) 36, 52, 93, 410
CviKI_1 RGCY 4 cut(s) 36, 52, 93, 410
CviQI GTAC 1 cut(s) 7
DdeI CTNAG 4 cut(s) 194, 218, 227, 378
DraIII CACNNNGTG 1 cut(s) 197
Ecl136II GAGCTC 1 cut(s) 52
Eco24I GRGCYC 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 52
EcoT38I GRGCYC 1 cut(s) 54
FaeI CATG 2 cut(s) 289, 319
FaiI YATR 4 cut(s) 171, 266, 287, 317
FatI CATG 2 cut(s) 285, 315
Fnu4HI GCNGC 1 cut(s) 201
FriOI GRGCYC 1 cut(s) 54
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 2 cut(s) 116, 261
GlaI GCGC 1 cut(s) 334
GluI GCNGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 321
HaeII RGCGCY 1 cut(s) 336
HapII CCGG 1 cut(s) 30
HhaI GCGC 1 cut(s) 335
Hin1II CATG 2 cut(s) 289, 319
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 384
HindII GTYRAC 1 cut(s) 384
HinfI GANTC 1 cut(s) 299
HpaII CCGG 1 cut(s) 30
Hpy166II GTNNAC 1 cut(s) 384
Hpy188I TCNGA 1 cut(s) 230
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 1 cut(s) 384
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4V TGCA 5 cut(s) 59, 128, 143, 191, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 125, 197
HpyF3I CTNAG 4 cut(s) 194, 218, 227, 378
Hsp92II CATG 2 cut(s) 289, 319
HspAI GCGC 1 cut(s) 333
LmnI GCTCC 2 cut(s) 115, 340
LpnPI CCDG 7 cut(s) 6, 43, 55, 60, 79, 309, 351
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 1 cut(s) 46
MaeI CTAG 2 cut(s) 116, 261
MaeIII GTNAC 2 cut(s) 221, 346
MboII GAAGA 1 cut(s) 55
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 2 cut(s) 82, 103
MnlI CCTC 4 cut(s) 20, 26, 236, 387
MseI TTAA 1 cut(s) 404
MspI CCGG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 116, 125, 197
NlaIII CATG 2 cut(s) 289, 319
NlaIV GGNNCC 2 cut(s) 255, 294
NmuCI GTSAC 1 cut(s) 221
PctI GAATGC 1 cut(s) 330
PfeI GAWTC 1 cut(s) 299
PkrI GCNGC 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 54
PspN4I GGNNCC 2 cut(s) 255, 294
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
SacI GAGCTC 1 cut(s) 54
SaqAI TTAA 1 cut(s) 404
SatI GCNGC 1 cut(s) 201
SduI GDGCHC 1 cut(s) 54
SetI ASST 5 cut(s) 12, 54, 95, 127, 228
SfaNI GCATC 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
Sse9I AATT 2 cut(s) 82, 103
SspMI CTAG 2 cut(s) 116, 261
SstI GAGCTC 1 cut(s) 54
TaaI ACNGT 1 cut(s) 388
TaqI TCGA 2 cut(s) 54, 75
TasI AATT 2 cut(s) 82, 103
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 221
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 3 cut(s) 17, 191, 332
TspRI CASTG 1 cut(s) 202
XapI RAATTY 2 cut(s) 82, 103
XspI CTAG 2 cut(s) 116, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.