pycom12g05590
ERF Family

Pectate lyase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013425
Physical Location & Seq
Forward (+)
5474180 .. 5476499
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
ATGGCACCTCCTCCGCCCCTCCTCATCCAATCACTCACTCTCCCGCTCCTATTCCTCCTCTTTCTGCTTCCTCCCCTCCAATCCCACTTCGCCACGTCACCCATCATCGAACTCCCCAACAACCCCACCACCACCATCTCCGTCGCCGACTACGGCGCCGTCGGCGACGGCCTCCGCTACGACACCATCGCCATCCAGTCCGCCATCGACAGCTGCCCCTCCCCTTCCCCCTGCCGCGTCACCTTCCCCGCGGGCCTCACCTACCTCACCGCGACCATCCGCCTCAAATCCGGCGTCGTATTAGACATCCAAGAAGGCGCGACGCTCCTGGGCGGCCCCAGGCTCGAGGACTACCCGAAGGAGCAGAGCAAGTGGTACGTGGTGCTGGCGGAGAACGCGATCAACGTCGGCATCAGCGGGGGCGGAGTGGTGGACGGTCAGGGGTTGAAGTTTGTGACGAGGTTCGATGAGAGGAAGAATGTGATGGTGAGCTGGAATTGGACCGGGGCTTGCTTGGGCGACGAGTGCCGTCCCAGGCTCGTCGGCTTCATCAACTGCCGGAATGTCAATCTCTCCAATCTCAAGCTGCGTCAGCCCGCTTACTGGTGCTTGCACATTGTACAGTGCGAGAATATTTTCATTGAAGATGTTTCAATCTATGGAGATTTCAACACACCCAACAATGACGGGATAGATATTGAGGATTCAAATAACACAGTCATTACCAGATGCCATATCAATACTGGAGATGATGCTATCTGTCCAAAGACGTACACTGGCCCCCTTTATAACTTGACTGTAACAAATTGTTGGATTCGAACAAAGTCTTCGGCAATCAAACTTGGCAGCGCTAGTTGGTTCAATTTCAAGGGTCTGGTGTTCGACAACATCACCATTGTGGATTCTCATAGAGGGCTGGGACTCCAAATACGCGATGGAGGAAATGTGAGTGACATTACCTTCTCAAACATAAATATACGCACAAGATACTATGATCGATCACCTAGCAGGCCTGGTGGGGAAGAGCGGAGCCAATCTACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

348

Amino Acids

38.15

Weight (kDa)

5.63

Isoelectric Point (pI)

55.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 46 - 101 1.6e-07 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 78 - 327 2.8e-23 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013425)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57790 AT3G57790
fragaria_vesca FvH4_6g22970
malus_domestica MD03G1162500.v1.1 MD12G1064100.v1.1
prunus_persica Prupe.7G070000_v2.0.a1
pyrus_communis pycom12g05590
rosa_chinensis RchiOBHm_Chr3g0480761
rosa_laevigata RLG00000023454
rosa_multiflora Rmu_co8289587.1_g000001 Rmu_sc0003902.1_g000012
rosa_roxburghii Rroxscaffold_6G00401090
rosa_rugosa Rorug03G0185600
rosa_samantha Rh3AG237700 Rh3BG271100 Rh3CG267200 Rh3DG263200
rosa_wichuraiana Rw3G021290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
AccB1I GGYRCC 2 cut(s) 4, 155
AccBSI CCGCTC 2 cut(s) 46, 1027
AccII CGCG 6 cut(s) 237, 251, 272, 320, 398, 933
AcyI GRCGYC 2 cut(s) 156, 294
AfaI GTAC 3 cut(s) 377, 621, 773
AfeI AGCGCT 1 cut(s) 850
AfiI CCNNNNNNNGG 1 cut(s) 603
AgsI TTSAA 7 cut(s) 448, 644, 654, 670, 708, 862, 868
AjiI CACGTC 1 cut(s) 96
AjnI CCWGG 4 cut(s) 327, 338, 533, 1012
AleI CACNNNNGTG 1 cut(s) 896
AluBI AGCT 3 cut(s) 213, 492, 586
AluI AGCT 3 cut(s) 213, 492, 586
Ama87I CYCGRG 1 cut(s) 344
Aor51HI AGCGCT 1 cut(s) 850
AoxI GGCC 5 cut(s) 169, 253, 334, 778, 1010
ApeKI GCWGC 3 cut(s) 213, 586, 846
ArsI GACNNNNNNTTYG 2 cut(s) 279, 311
Asp700I GAANNNNTTC 1 cut(s) 635
AspLEI GCGC 3 cut(s) 158, 320, 851
AspS9I GGNCC 4 cut(s) 253, 335, 501, 779
AsuC2I CCSGG 1 cut(s) 505
AsuHPI GGTGA 7 cut(s) 90, 232, 250, 259, 499, 883, 993
AsuII TTCGAA 1 cut(s) 817
AvaI CYCGRG 1 cut(s) 344
AvaII GGWCC 1 cut(s) 501
BanI GGYRCC 2 cut(s) 4, 155
BbsI GAAGAC 1 cut(s) 819
BbvI GCAGC 3 cut(s) 200, 573, 858
BccI CCATC 8 cut(s) 110, 143, 194, 200, 212, 284, 478, 929
BceAI ACGGC 4 cut(s) 143, 169, 184, 513
BciT130I CCWGG 4 cut(s) 329, 340, 535, 1014
BcnI CCSGG 1 cut(s) 505
BfaI CTAG 2 cut(s) 852, 1005
BfoI RGCGCY 2 cut(s) 159, 852
BisI GCNGC 5 cut(s) 214, 235, 334, 587, 847
BlsI GCNGC 5 cut(s) 215, 236, 335, 588, 848
Bme1390I CCNGG 5 cut(s) 329, 340, 505, 535, 1014
Bme18I GGWCC 1 cut(s) 501
BmeT110I CYCGRG 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 96
BmgT120I GGNCC 4 cut(s) 253, 335, 501, 779
BmiI GGNNCC 5 cut(s) 6, 157, 337, 781, 1031
BmrFI CCNGG 5 cut(s) 329, 340, 505, 535, 1014
BmsI GCATC 3 cut(s) 420, 719, 742
BpiI GAAGAC 1 cut(s) 819
BpmI CTGGAG 1 cut(s) 765
Bpu14I TTCGAA 1 cut(s) 817
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 505
Bsa29I ATCGAT 1 cut(s) 997
BsaAI YACGTR 2 cut(s) 379, 1041
BsaHI GRCGYC 2 cut(s) 156, 294
BsaJI CCNNGG 5 cut(s) 249, 328, 338, 504, 533
BsaXI ACNNNNNCTCC 4 cut(s) 24, 54, 930, 960
Bsc4I CCNNNNNNNGG 1 cut(s) 603
Bse1I ACTGG 4 cut(s) 196, 608, 748, 781
BseBI CCWGG 4 cut(s) 329, 340, 535, 1014
BseCI ATCGAT 1 cut(s) 997
BseDI CCNNGG 5 cut(s) 249, 328, 338, 504, 533
BseGI GGATG 4 cut(s) 24, 192, 276, 306
BseLI CCNNNNNNNGG 1 cut(s) 603
BseNI ACTGG 4 cut(s) 196, 608, 748, 781
BseRI GAGGAG 2 cut(s) 11, 47
BseXI GCAGC 3 cut(s) 200, 573, 858
BseYI CCCAGC 1 cut(s) 916
Bsh1236I CGCG 6 cut(s) 237, 251, 272, 320, 398, 933
BshFI GGCC 5 cut(s) 171, 255, 336, 780, 1012
BshNI GGYRCC 2 cut(s) 4, 155
BshVI ATCGAT 1 cut(s) 997
BsiHKCI CYCGRG 1 cut(s) 344
BsiSI CCGG 3 cut(s) 291, 504, 559
BslFI GGGAC 2 cut(s) 516, 933
BslI CCNNNNNNNGG 1 cut(s) 603
BsmFI GGGAC 2 cut(s) 516, 933
BsnI GGCC 5 cut(s) 171, 255, 336, 780, 1012
BsoBI CYCGRG 1 cut(s) 344
Bsp119I TTCGAA 1 cut(s) 817
Bsp1407I TGTACA 1 cut(s) 619
Bsp143I GATC 3 cut(s) 399, 994, 998
BspANI GGCC 5 cut(s) 171, 255, 336, 780, 1012
BspDI ATCGAT 1 cut(s) 997
BspFNI CGCG 6 cut(s) 237, 251, 272, 320, 398, 933
BspLI GGNNCC 5 cut(s) 6, 157, 337, 781, 1031
BspQI GCTCTTC 1 cut(s) 1017
BspT104I TTCGAA 1 cut(s) 817
BspT107I GGYRCC 2 cut(s) 4, 155
BsrBI CCGCTC 2 cut(s) 46, 1027
BsrGI TGTACA 1 cut(s) 619
BsrI ACTGG 4 cut(s) 196, 608, 748, 781
BssECI CCNNGG 5 cut(s) 249, 328, 338, 504, 533
BssMI GATC 3 cut(s) 399, 994, 998
BssNI GRCGYC 2 cut(s) 156, 294
Bst2UI CCWGG 4 cut(s) 329, 340, 535, 1014
Bst4CI ACNGT 4 cut(s) 437, 624, 718, 799
Bst6I CTCTTC 1 cut(s) 1017
BstACI GRCGYC 2 cut(s) 156, 294
BstAUI TGTACA 1 cut(s) 619
BstBAI YACGTR 2 cut(s) 379, 1041
BstBI TTCGAA 1 cut(s) 817
BstC8I GCNNGC 6 cut(s) 253, 387, 511, 597, 611, 1010
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 4 cut(s) 24, 192, 276, 306
BstFNI CGCG 6 cut(s) 237, 251, 272, 320, 398, 933
BstH2I RGCGCY 2 cut(s) 159, 852
BstHHI GCGC 3 cut(s) 158, 320, 851
BstKTI GATC 3 cut(s) 402, 997, 1001
BstMBI GATC 3 cut(s) 399, 994, 998
BstMWI GCNNNNNNNGC 4 cut(s) 162, 395, 525, 592
BstNI CCWGG 4 cut(s) 329, 340, 535, 1014
BstSCI CCNGG 5 cut(s) 327, 338, 503, 533, 1012
BstUI CGCG 6 cut(s) 237, 251, 272, 320, 398, 933
BstV1I GCAGC 3 cut(s) 200, 573, 858
BstV2I GAAGAC 1 cut(s) 819
Bsu15I ATCGAT 1 cut(s) 997
BsuRI GGCC 5 cut(s) 171, 255, 336, 780, 1012
BsuTUI ATCGAT 1 cut(s) 997
BtgI CCRYGG 1 cut(s) 249
BtgZI GCGATG 2 cut(s) 172, 948
BtrI CACGTC 1 cut(s) 96
BtsCI GGATG 4 cut(s) 24, 192, 276, 306
BtsIMutI CAGTG 2 cut(s) 629, 774
Cac8I GCNNGC 6 cut(s) 253, 387, 511, 597, 611, 1010
CfoI GCGC 3 cut(s) 158, 320, 851
Cfr13I GGNCC 4 cut(s) 253, 335, 501, 779
Cfr42I CCGCGG 1 cut(s) 252
ClaI ATCGAT 1 cut(s) 997
CseI GACGC 4 cut(s) 226, 283, 331, 578
Csp6I GTAC 3 cut(s) 376, 620, 772
CviQI GTAC 3 cut(s) 376, 620, 772
DinI GGCGCC 1 cut(s) 157
DpnI GATC 3 cut(s) 401, 996, 1000
DpnII GATC 3 cut(s) 399, 994, 998
Eam1104I CTCTTC 1 cut(s) 1017
EarI CTCTTC 1 cut(s) 1017
EciI GGCGGA 4 cut(s) 190, 269, 404, 438
Eco147I AGGCCT 1 cut(s) 1012
Eco47I GGWCC 1 cut(s) 501
Eco47III AGCGCT 1 cut(s) 850
Eco88I CYCGRG 1 cut(s) 344
EcoRII CCWGG 4 cut(s) 327, 338, 533, 1012
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
FaiI YATR 7 cut(s) 660, 735, 789, 909, 971, 977, 993
FaqI GGGAC 2 cut(s) 516, 933
FauI CCCGC 5 cut(s) 51, 244, 256, 410, 604
Fnu4HI GCNGC 5 cut(s) 214, 235, 334, 587, 847
FokI GGATG 4 cut(s) 11, 179, 263, 293
Fsp4HI GCNGC 5 cut(s) 214, 235, 334, 587, 847
FspBI CTAG 2 cut(s) 852, 1005
GlaI GCGC 3 cut(s) 157, 319, 850
GluI GCNGC 5 cut(s) 214, 235, 334, 587, 847
GsaI CCCAGC 1 cut(s) 920
GsuI CTGGAG 1 cut(s) 765
HaeII RGCGCY 2 cut(s) 159, 852
HaeIII GGCC 5 cut(s) 171, 255, 336, 780, 1012
HapII CCGG 3 cut(s) 291, 504, 559
HgaI GACGC 4 cut(s) 226, 283, 331, 578
HhaI GCGC 3 cut(s) 158, 320, 851
Hin1I GRCGYC 2 cut(s) 156, 294
Hin6I GCGC 3 cut(s) 156, 318, 849
HinP1I GCGC 3 cut(s) 156, 318, 849
HinfI GANTC 4 cut(s) 704, 814, 902, 921
HpaII CCGG 3 cut(s) 291, 504, 559
HphI GGTGA 7 cut(s) 90, 232, 250, 259, 499, 883, 993
Hpy166II GTNNAC 2 cut(s) 433, 774
Hpy8I GTNNAC 2 cut(s) 433, 774
Hpy99I CGWCG 8 cut(s) 146, 164, 170, 299, 325, 410, 524, 545
HpyAV CCTTC 5 cut(s) 234, 253, 308, 352, 970
HpyCH4III ACNGT 4 cut(s) 437, 624, 718, 799
HpyCH4IV ACGT 5 cut(s) 95, 378, 405, 770, 1040
HpyCH4V TGCA 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 395, 525, 592
HpySE526I ACGT 5 cut(s) 95, 378, 405, 770, 1040
Hsp92I GRCGYC 2 cut(s) 156, 294
HspAI GCGC 3 cut(s) 156, 318, 849
KasI GGCGCC 1 cut(s) 155
KspI CCGCGG 1 cut(s) 252
Kzo9I GATC 3 cut(s) 399, 994, 998
LguI GCTCTTC 1 cut(s) 1017
LmnI GCTCC 4 cut(s) 51, 330, 361, 1029
Lsp1109I GCAGC 3 cut(s) 200, 573, 858
LweI GCATC 3 cut(s) 420, 719, 742
MaeI CTAG 2 cut(s) 852, 1005
MaeII ACGT 5 cut(s) 95, 378, 405, 770, 1040
MaeIII GTNAC 5 cut(s) 96, 238, 454, 799, 950
MalI GATC 3 cut(s) 401, 996, 1000
MbiI CCGCTC 2 cut(s) 46, 1027
MboI GATC 3 cut(s) 399, 994, 998
MboII GAAGA 4 cut(s) 487, 656, 819, 1034
MluCI AATT 3 cut(s) 496, 805, 862
Mly113I GGCGCC 1 cut(s) 156
MlyI GAGTC 1 cut(s) 915
MmeI TCCRAC 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 635
MslI CAYNNNNRTG 1 cut(s) 896
MspA1I CMGCKG 3 cut(s) 213, 251, 417
MspI CCGG 3 cut(s) 291, 504, 559
MspR9I CCNGG 5 cut(s) 329, 340, 505, 535, 1014
MvaI CCWGG 4 cut(s) 329, 340, 535, 1014
MvnI CGCG 6 cut(s) 237, 251, 272, 320, 398, 933
MwoI GCNNNNNNNGC 4 cut(s) 162, 395, 525, 592
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 1 cut(s) 505
NdeII GATC 3 cut(s) 399, 994, 998
NlaIV GGNNCC 5 cut(s) 6, 157, 337, 781, 1031
NmuCI GTSAC 4 cut(s) 96, 238, 454, 950
NspV TTCGAA 1 cut(s) 817
OliI CACNNNNGTG 1 cut(s) 896
PaeR7I CTCGAG 1 cut(s) 344
PceI AGGCCT 1 cut(s) 1012
PciSI GCTCTTC 1 cut(s) 1017
PcsI WCGNNNNNNNCGW 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 635
PfeI GAWTC 3 cut(s) 704, 814, 902
PkrI GCNGC 5 cut(s) 215, 236, 335, 588, 848
PleI GAGTC 1 cut(s) 915
PluTI GGCGCC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 915
Ppu21I YACGTR 2 cut(s) 379, 1041
PsiI TTATAA 1 cut(s) 789
Psp6I CCWGG 4 cut(s) 327, 338, 533, 1012
PspFI CCCAGC 1 cut(s) 916
PspGI CCWGG 4 cut(s) 327, 338, 533, 1012
PspN4I GGNNCC 5 cut(s) 6, 157, 337, 781, 1031
PspPI GGNCC 4 cut(s) 253, 335, 501, 779
PspXI VCTCGAGB 1 cut(s) 344
PvuII CAGCTG 1 cut(s) 213
RsaI GTAC 3 cut(s) 377, 621, 773
RsaNI GTAC 3 cut(s) 376, 620, 772
RseI CAYNNNNRTG 1 cut(s) 896
SacII CCGCGG 1 cut(s) 252
SapI GCTCTTC 1 cut(s) 1017
SatI GCNGC 5 cut(s) 214, 235, 334, 587, 847
Sau3AI GATC 3 cut(s) 399, 994, 998
Sau96I GGNCC 4 cut(s) 253, 335, 501, 779
SchI GAGTC 1 cut(s) 915
ScrFI CCNGG 5 cut(s) 329, 340, 505, 535, 1014
SfaNI GCATC 3 cut(s) 420, 719, 742
SfoI GGCGCC 1 cut(s) 157
Sfr274I CTCGAG 1 cut(s) 344
Sfr303I CCGCGG 1 cut(s) 252
SfuI TTCGAA 1 cut(s) 817
SgrBI CCGCGG 1 cut(s) 252
SinI GGWCC 1 cut(s) 501
SlaI CTCGAG 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 896
SmlI CTYRAG 2 cut(s) 344, 581
SmoI CTYRAG 2 cut(s) 344, 581
Sse9I AATT 3 cut(s) 496, 805, 862
SseBI AGGCCT 1 cut(s) 1012
SspDI GGCGCC 1 cut(s) 155
SspI AATATT 1 cut(s) 634
SspMI CTAG 2 cut(s) 852, 1005
StuI AGGCCT 1 cut(s) 1012
StyD4I CCNGG 5 cut(s) 327, 338, 503, 533, 1012
TaaI ACNGT 4 cut(s) 437, 624, 718, 799
TaiI ACGT 5 cut(s) 98, 381, 408, 773, 1043
TaqI TCGA 7 cut(s) 108, 207, 345, 465, 817, 882, 997
TasI AATT 3 cut(s) 496, 805, 862
TatI WGTACW 1 cut(s) 619
TauI GCSGC 2 cut(s) 237, 336
TfiI GAWTC 3 cut(s) 704, 814, 902
TscAI CASTG 2 cut(s) 629, 781
TseFI GTSAC 4 cut(s) 96, 238, 454, 950
TseI GCWGC 3 cut(s) 213, 586, 846
Tsp45I GTSAC 4 cut(s) 96, 238, 454, 950
TspDTI ATGAA 2 cut(s) 538, 628
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 2 cut(s) 629, 781
VpaK11BI GGWCC 1 cut(s) 501
XcmI CCANNNNNNNNNTGG 2 cut(s) 740, 932
XhoI CTCGAG 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 635
XspI CTAG 2 cut(s) 852, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.