pycom12g07670

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012918
Physical Location & Seq
Reverse (-)
8243619 .. 8246388
2770 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 750 bp
ATGGGTAGTTCAGCCGACGGAGAGGATTTCTCAGTGGTGGTTCTGGCGTCCGATCTGGGCGTAGACGCGCGGCCCTTTTTGGCGAACCAAGACAGAGAGATTGAAGAGCAAGAGAATTGGTACGACTGCCCTCAGAACCTTATCTCCGACGAGGACTTTTCCGATCTCGAGCTTTTGCAGTTCTTTCGTGTCCAGGGCCATGACAAGTCTGGAAATCGTATTTTCCGCATCGTCGGAAAATATTTTCCCGCTCCAGTTGTTAGTGCAGACCGGCTGAAGAGGTATATTTTCCACAAATTATGCAGTGAGGTCCCAGAAGGGCCATACTCCATTGTTTACATGCATAGTACTGTGCAAAAGGAAGATAACTCCCCTGGCATAACTATCTTGCGGTGGCTTTATGAAGACCTTCCATCATACTTGAAGGACAGGCTTCAAGTTGTGTACTTTGTTCACCCTGGGCTCCGATCAAGACTTGTCTTTGCCACTCTTGGCCGATTTTTCTTAACTGGAGGCCTATATTGGAAAATCAAGTATGTAAGCCGGCTGCAGTACCTATGGGATGATATAAAGAAAGGGGAGGTCGAGATTCCTGAATTTGTCAAAAATCATGACGATGTTCTTGAGCATAGACCACTAACAGACTATGGCATCGAACCGGATCCTCTTCACATGCTTGAGGCTCCATCAACAGCCTACTCATTTGGAAGGTATGAGGAGAGATGGACGTCAAGAGAGTATATGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

250

Amino Acids

29.09

Weight (kDa)

5.06

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 71 - 206 6.4e-33 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10210
fragaria_vesca FvH4_6g25120
malus_domestica MD00G1048900.v1.1 MD00G1074100.v1.1
prunus_persica Prupe.7G046200_v2.0.a1 Prupe.7G046200_v2.0.a1
pyrus_communis pycom12g07670
rosa_chinensis RchiOBHm_Chr2g0124961
rosa_laevigata RLG00000018840
rosa_multiflora Rmu_sc0008068.1_g000005
rosa_roxburghii Rroxscaffold_2G00118490 Rroxscaffold_2G00118790
rosa_rugosa Rorug02G0254500
rosa_samantha Rh2AG313100 Rh2BG321300 Rh2CG302600 Rh2DG338700
rosa_wichuraiana Rw2G025290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 731
AccBSI CCGCTC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 63
AccII CGCG 2 cut(s) 68, 70
AciI CCGC 4 cut(s) 70, 226, 249, 391
AclWI GGATC 2 cut(s) 656, 669
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 596
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 2 cut(s) 47, 728
AfaI GTAC 4 cut(s) 122, 349, 446, 554
AgsI TTSAA 3 cut(s) 104, 424, 437
AjnI CCWGG 3 cut(s) 192, 373, 457
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 1 cut(s) 172
AluI AGCT 1 cut(s) 172
AlwI GGATC 2 cut(s) 656, 669
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 5 cut(s) 71, 196, 320, 493, 514
ApeKI GCWGC 1 cut(s) 547
ApoI RAATTY 1 cut(s) 596
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 4 cut(s) 72, 196, 310, 320
AsuHPI GGTGA 1 cut(s) 446
AvaI CYCGRG 1 cut(s) 167
AvaII GGWCC 1 cut(s) 310
BamHI GGATCC 1 cut(s) 661
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 534
BccI CCATC 3 cut(s) 421, 694, 717
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BciT130I CCWGG 3 cut(s) 194, 375, 459
BfmI CTRYAG 1 cut(s) 548
BisI GCNGC 2 cut(s) 71, 548
BlsI GCNGC 2 cut(s) 72, 549
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 3 cut(s) 194, 375, 459
Bme18I GGWCC 1 cut(s) 310
BmeT110I CYCGRG 1 cut(s) 167
BmgT120I GGNCC 4 cut(s) 72, 196, 310, 320
BmiI GGNNCC 4 cut(s) 312, 464, 663, 684
BmrFI CCNGG 3 cut(s) 194, 375, 459
BmsI GCATC 2 cut(s) 237, 660
BpiI GAAGAC 1 cut(s) 411
BplI GAGNNNNNCTC 2 cut(s) 14, 46
BpmI CTGGAG 2 cut(s) 237, 531
BpuEI CTTGAG 2 cut(s) 644, 698
BsaHI GRCGYC 2 cut(s) 47, 728
BsaJI CCNNGG 4 cut(s) 193, 373, 457, 458
BsaWI WCCGGW 1 cut(s) 658
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bse118I RCCGGY 2 cut(s) 270, 543
Bse1I ACTGG 2 cut(s) 254, 514
BseBI CCWGG 3 cut(s) 194, 375, 459
BseDI CCNNGG 4 cut(s) 193, 373, 457, 458
BseGI GGATG 1 cut(s) 568
BseMII CTCAG 2 cut(s) 45, 146
BseNI ACTGG 2 cut(s) 254, 514
BseRI GAGGAG 1 cut(s) 731
BseXI GCAGC 1 cut(s) 534
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 2 cut(s) 68, 70
BshFI GGCC 5 cut(s) 73, 198, 322, 495, 516
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 3 cut(s) 271, 544, 659
BslFI GGGAC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 296
BsnI GGCC 5 cut(s) 73, 198, 322, 495, 516
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 4 cut(s) 52, 163, 467, 661
BspACI CCGC 4 cut(s) 70, 226, 249, 391
BspANI GGCC 5 cut(s) 73, 198, 322, 495, 516
BspCNI CTCAG 2 cut(s) 44, 145
BspFNI CGCG 2 cut(s) 68, 70
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 4 cut(s) 312, 464, 663, 684
BspMAI CTGCAG 1 cut(s) 552
BspPI GGATC 2 cut(s) 656, 669
BspQI GCTCTTC 1 cut(s) 99
BsrBI CCGCTC 1 cut(s) 251
BsrFI RCCGGY 2 cut(s) 270, 543
BsrI ACTGG 2 cut(s) 254, 514
BssAI RCCGGY 2 cut(s) 270, 543
BssECI CCNNGG 4 cut(s) 193, 373, 457, 458
BssMI GATC 4 cut(s) 52, 163, 467, 661
BssNI GRCGYC 2 cut(s) 47, 728
Bst2UI CCWGG 3 cut(s) 194, 375, 459
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 3 cut(s) 99, 272, 672
BstACI GRCGYC 2 cut(s) 47, 728
BstC8I GCNNGC 1 cut(s) 545
BstDEI CTNAG 2 cut(s) 31, 132
BstF5I GGATG 1 cut(s) 568
BstFNI CGCG 2 cut(s) 68, 70
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 4 cut(s) 55, 166, 470, 664
BstMBI GATC 4 cut(s) 52, 163, 467, 661
BstNI CCWGG 3 cut(s) 194, 375, 459
BstNSI RCATGY 2 cut(s) 343, 676
BstSCI CCNGG 3 cut(s) 192, 373, 457
BstSFI CTRYAG 1 cut(s) 548
BstUI CGCG 2 cut(s) 68, 70
BstV1I GCAGC 1 cut(s) 534
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 661
BstYI RGATCY 1 cut(s) 661
BsuRI GGCC 5 cut(s) 73, 198, 322, 495, 516
BtsCI GGATG 1 cut(s) 568
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 2 cut(s) 39, 310
Cac8I GCNNGC 1 cut(s) 545
CciI TCATGA 1 cut(s) 610
CfoI GCGC 1 cut(s) 70
Cfr10I RCCGGY 2 cut(s) 270, 543
Cfr13I GGNCC 4 cut(s) 72, 196, 310, 320
CseI GACGC 2 cut(s) 36, 74
Csp6I GTAC 4 cut(s) 121, 348, 445, 553
CviAII CATG 4 cut(s) 200, 340, 611, 673
CviQI GTAC 4 cut(s) 121, 348, 445, 553
DdeI CTNAG 2 cut(s) 31, 132
DpnI GATC 4 cut(s) 54, 165, 469, 663
DpnII GATC 4 cut(s) 52, 163, 467, 661
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 3 cut(s) 99, 272, 672
EarI CTCTTC 3 cut(s) 99, 272, 672
Eco147I AGGCCT 1 cut(s) 516
Eco24I GRGCYC 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 310
EcoRII CCWGG 3 cut(s) 192, 373, 457
EcoT22I ATGCAT 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 465
FaeI CATG 4 cut(s) 203, 343, 614, 676
FaqI GGGAC 1 cut(s) 296
FatI CATG 4 cut(s) 199, 339, 610, 672
FauI CCCGC 1 cut(s) 256
FblI GTMKAC 1 cut(s) 63
Fnu4HI GCNGC 2 cut(s) 71, 548
FokI GGATG 1 cut(s) 575
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 2 cut(s) 71, 548
GlaI GCGC 1 cut(s) 69
GluI GCNGC 2 cut(s) 71, 548
GsuI CTGGAG 2 cut(s) 237, 531
HaeIII GGCC 5 cut(s) 73, 198, 322, 495, 516
HapII CCGG 3 cut(s) 271, 544, 659
HgaI GACGC 2 cut(s) 36, 74
HhaI GCGC 1 cut(s) 70
Hin1I GRCGYC 2 cut(s) 47, 728
Hin1II CATG 4 cut(s) 203, 343, 614, 676
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HinfI GANTC 1 cut(s) 589
HpaII CCGG 3 cut(s) 271, 544, 659
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 4 cut(s) 64, 337, 445, 454
Hpy188I TCNGA 6 cut(s) 52, 135, 148, 163, 236, 467
Hpy188III TCNNGA 8 cut(s) 167, 210, 471, 586, 593, 611, 623, 732
Hpy8I GTNNAC 4 cut(s) 64, 337, 445, 454
Hpy99I CGWCG 3 cut(s) 20, 152, 236
HpyAV CCTTC 4 cut(s) 311, 418, 419, 702
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 1 cut(s) 728
HpyCH4V TGCA 6 cut(s) 178, 266, 303, 343, 355, 550
HpyF3I CTNAG 2 cut(s) 31, 132
HpySE526I ACGT 1 cut(s) 728
Hsp92I GRCGYC 2 cut(s) 47, 728
Hsp92II CATG 4 cut(s) 203, 343, 614, 676
HspAI GCGC 1 cut(s) 68
KroI GCCGGC 1 cut(s) 543
KroNI GCCGGC 1 cut(s) 545
Kzo9I GATC 4 cut(s) 52, 163, 467, 661
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 3 cut(s) 256, 468, 688
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 2 cut(s) 237, 660
MaeII ACGT 1 cut(s) 728
MalI GATC 4 cut(s) 54, 165, 469, 663
MbiI CCGCTC 1 cut(s) 251
MboI GATC 4 cut(s) 52, 163, 467, 661
MboII GAAGA 5 cut(s) 116, 289, 374, 416, 659
MflI RGATCY 1 cut(s) 661
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 3 cut(s) 115, 296, 596
MmeI TCCRAC 2 cut(s) 171, 214
Mph1103I ATGCAT 1 cut(s) 345
MroNI GCCGGC 1 cut(s) 543
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 1 cut(s) 506
MslI CAYNNNNRTG 1 cut(s) 615
MspI CCGG 3 cut(s) 271, 544, 659
MspR9I CCNGG 3 cut(s) 194, 375, 459
MvaI CCWGG 3 cut(s) 194, 375, 459
MvnI CGCG 2 cut(s) 68, 70
NaeI GCCGGC 1 cut(s) 545
NdeII GATC 4 cut(s) 52, 163, 467, 661
NgoMIV GCCGGC 1 cut(s) 543
NlaIII CATG 4 cut(s) 203, 343, 614, 676
NlaIV GGNNCC 4 cut(s) 312, 464, 663, 684
NsiI ATGCAT 1 cut(s) 345
NspI RCATGY 2 cut(s) 343, 676
PaeR7I CTCGAG 1 cut(s) 167
PagI TCATGA 1 cut(s) 610
PasI CCCWGGG 1 cut(s) 458
PceI AGGCCT 1 cut(s) 516
PciSI GCTCTTC 1 cut(s) 99
PdiI GCCGGC 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 1 cut(s) 589
PkrI GCNGC 2 cut(s) 72, 549
PpuMI RGGWCCY 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 3 cut(s) 192, 373, 457
PspGI CCWGG 3 cut(s) 192, 373, 457
PspN4I GGNNCC 4 cut(s) 312, 464, 663, 684
PspPI GGNCC 4 cut(s) 72, 196, 310, 320
PspPPI RGGWCCY 1 cut(s) 310
PstI CTGCAG 1 cut(s) 552
PsuI RGATCY 1 cut(s) 661
RsaI GTAC 4 cut(s) 122, 349, 446, 554
RsaNI GTAC 4 cut(s) 121, 348, 445, 553
RseI CAYNNNNRTG 1 cut(s) 615
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 1 cut(s) 506
SatI GCNGC 2 cut(s) 71, 548
Sau3AI GATC 4 cut(s) 52, 163, 467, 661
Sau96I GGNCC 4 cut(s) 72, 196, 310, 320
ScaI AGTACT 1 cut(s) 349
ScrFI CCNGG 3 cut(s) 194, 375, 459
SduI GDGCHC 1 cut(s) 465
SetI ASST 9 cut(s) 141, 174, 284, 312, 411, 558, 585, 713, 731
SfaNI GCATC 2 cut(s) 237, 660
SfcI CTRYAG 1 cut(s) 548
Sfr274I CTCGAG 1 cut(s) 167
SinI GGWCC 1 cut(s) 310
SlaI CTCGAG 1 cut(s) 167
SmiMI CAYNNNNRTG 1 cut(s) 615
SmlI CTYRAG 3 cut(s) 167, 623, 677
SmoI CTYRAG 3 cut(s) 167, 623, 677
Sse9I AATT 3 cut(s) 115, 296, 596
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 4 cut(s) 70, 226, 249, 391
SspI AATATT 1 cut(s) 242
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 3 cut(s) 192, 373, 457
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 1 cut(s) 731
TaqI TCGA 3 cut(s) 168, 585, 654
TasI AATT 3 cut(s) 115, 296, 596
TatI WGTACW 2 cut(s) 347, 444
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 1 cut(s) 589
Tru1I TTAA 1 cut(s) 506
Tru9I TTAA 1 cut(s) 506
TscAI CASTG 2 cut(s) 39, 310
TseI GCWGC 1 cut(s) 547
TspDTI ATGAA 1 cut(s) 417
TspGWI ACGGA 1 cut(s) 33
TspRI CASTG 2 cut(s) 39, 310
VpaK11BI GGWCC 1 cut(s) 310
XapI RAATTY 1 cut(s) 596
XceI RCATGY 2 cut(s) 343, 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 206
XhoI CTCGAG 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 408
ZraI GACGTC 1 cut(s) 729
ZrmI AGTACT 1 cut(s) 349
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.