pycom12g14890

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011575
Physical Location & Seq
Reverse (-)
17358017 .. 17360439
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ATGTCCCAAGTCGACTTAGAGCAAGGGCCCCACCGCCGCTCCTTCTCCGGAAGCGCTGACGGCGGAAGCAGCGTCTGCTTCTCCGACGCAGACGACGGGTCCTGTTACACTCAGTTCTTCTCGACCAATGGCGGCTCCTACGACGAGTACAATTTTGCGTCTCAGATCGGCGTGGTGTCGGATTCCCGGAGAGCCTCGTCTGTTTCGGACAGTTCGGTCGAGGTTGTCGAAATTGAGATTGGTGTGCCGGAAATTAAGGTGCATTTGGATAGAGTAGAGAGAGATTGTCGGATTTGTCATCTGGGTTTGGAGAGTAACAGCCACGAATCCGGGGCTCCGATTGAATTGGGTTGTTCTTGCAAGGATGATTTGGCTGCTGCCCACAAGAACTGTGCTGATACGTGGTTCAAGATTAAGGGCAACAAGACCTGTGAGATATGCCAGGCAGTTGCACGCAACGTCGTTGGGCCAAATGACATTGAGTTCACAGAGCAGTCGAACGAATCCAGTACTGCCTCAGCAACAGCTTCAGTTGCCGCCCCAGGGCCGTCCACGGAAACTCAAAGTTTCTGGCACGGTCACCGCTTCCTCAACTTCCTTCTTGCCTGCATGGTATTTGCTTTTGTCATATCATGGCTCTTTCACTTCAACGTTCCGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

221

Amino Acids

23.81

Weight (kDa)

4.82

Isoelectric Point (pI)

53.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 96 - 147 2e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 215
AccBSI CCGCTC 1 cut(s) 39
AccI GTMKAC 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 47
AciI CCGC 6 cut(s) 34, 37, 63, 132, 537, 583
AclI AACGTT 1 cut(s) 651
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 2 cut(s) 149, 511
AfeI AGCGCT 1 cut(s) 55
AfiI CCNNNNNNNGG 1 cut(s) 543
AgsI TTSAA 3 cut(s) 344, 409, 649
AhdI GACNNNNNGTC 1 cut(s) 97
AjnI CCWGG 2 cut(s) 441, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 222, 254
AluBI AGCT 1 cut(s) 527
AluI AGCT 1 cut(s) 527
Alw26I GTCTC 1 cut(s) 165
AlwNI CAGNNNCTG 1 cut(s) 75
Aor13HI TCCGGA 1 cut(s) 47
Aor51HI AGCGCT 1 cut(s) 55
AoxI GGCC 3 cut(s) 26, 467, 545
ApaI GGGCCC 1 cut(s) 30
ApeKI GCWGC 3 cut(s) 69, 374, 377
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 5 cut(s) 26, 27, 99, 467, 545
AsuC2I CCSGG 2 cut(s) 187, 331
AsuHPI GGTGA 1 cut(s) 572
AvaII GGWCC 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 30, 337
BbvCI CCTCAGC 1 cut(s) 517
BbvI GCAGC 3 cut(s) 81, 361, 364
BceAI ACGGC 2 cut(s) 76, 532
BciT130I CCWGG 2 cut(s) 443, 543
BcnI CCSGG 2 cut(s) 187, 331
BcoDI GTCTC 1 cut(s) 165
BfoI RGCGCY 1 cut(s) 57
BisI GCNGC 6 cut(s) 37, 70, 133, 375, 378, 537
BlsI GCNGC 6 cut(s) 38, 71, 134, 376, 379, 538
BmcAI AGTACT 1 cut(s) 511
Bme1390I CCNGG 4 cut(s) 187, 331, 443, 543
Bme18I GGWCC 1 cut(s) 99
BmeRI GACNNNNNGTC 1 cut(s) 97
BmgT120I GGNCC 5 cut(s) 26, 27, 99, 467, 545
BmiI GGNNCC 5 cut(s) 28, 29, 100, 136, 336
BmrFI CCNGG 4 cut(s) 187, 331, 443, 543
Bpu10I CCTNAGC 1 cut(s) 517
BpuMI CCSGG 2 cut(s) 187, 331
BsaAI YACGTR 1 cut(s) 402
BsaJI CCNNGG 4 cut(s) 330, 541, 542, 552
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 1 cut(s) 543
Bse1I ACTGG 1 cut(s) 507
BseAI TCCGGA 1 cut(s) 47
BseBI CCWGG 2 cut(s) 443, 543
BseDI CCNNGG 4 cut(s) 330, 541, 542, 552
BseGI GGATG 1 cut(s) 370
BseLI CCNNNNNNNGG 1 cut(s) 543
BseMII CTCAG 3 cut(s) 125, 176, 531
BseNI ACTGG 1 cut(s) 507
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 3 cut(s) 81, 361, 364
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 28, 469, 547
BsiEI CGRYCG 1 cut(s) 219
BsiSI CCGG 4 cut(s) 48, 187, 248, 330
BslI CCNNNNNNNGG 1 cut(s) 543
BsmAI GTCTC 1 cut(s) 165
BsmBI CGTCTC 1 cut(s) 165
BsnI GGCC 3 cut(s) 28, 469, 547
Bsp120I GGGCCC 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 30, 337
Bsp13I TCCGGA 1 cut(s) 47
Bsp143I GATC 1 cut(s) 165
BspACI CCGC 6 cut(s) 34, 37, 63, 132, 537, 583
BspANI GGCC 3 cut(s) 28, 469, 547
BspCNI CTCAG 3 cut(s) 124, 175, 530
BspEI TCCGGA 1 cut(s) 47
BspLI GGNNCC 5 cut(s) 28, 29, 100, 136, 336
BsrBI CCGCTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 507
BssECI CCNNGG 4 cut(s) 330, 541, 542, 552
BssMI GATC 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 443, 543
Bst4CI ACNGT 3 cut(s) 212, 392, 578
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 402
BstC8I GCNNGC 2 cut(s) 454, 607
BstDEI CTNAG 4 cut(s) 16, 111, 162, 517
BstDSI CCRYGG 1 cut(s) 552
BstEII GGTNACC 1 cut(s) 578
BstF5I GGATG 1 cut(s) 370
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 1 cut(s) 168
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 1 cut(s) 165
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 4 cut(s) 60, 69, 75, 533
BstNI CCWGG 2 cut(s) 443, 543
BstPI GGTNACC 1 cut(s) 578
BstSCI CCNGG 4 cut(s) 185, 329, 441, 541
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 81, 361, 364
BsuRI GGCC 3 cut(s) 28, 469, 547
BtgI CCRYGG 1 cut(s) 552
BtsCI GGATG 1 cut(s) 370
Cac8I GCNNGC 2 cut(s) 454, 607
CaiI CAGNNNCTG 1 cut(s) 75
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 5 cut(s) 26, 27, 99, 467, 545
CseI GACGC 3 cut(s) 61, 95, 147
Csp6I GTAC 2 cut(s) 148, 510
CviAII CATG 2 cut(s) 610, 633
CviQI GTAC 2 cut(s) 148, 510
DdeI CTNAG 4 cut(s) 16, 111, 162, 517
DpnI GATC 1 cut(s) 167
DpnII GATC 1 cut(s) 165
DrdI GACNNNNNNGTC 1 cut(s) 215
DriI GACNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 215
Eam1105I GACNNNNNGTC 1 cut(s) 97
EciI GGCGGA 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 30, 337
Eco47I GGWCC 1 cut(s) 99
Eco47III AGCGCT 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 513
Eco91I GGTNACC 1 cut(s) 578
EcoO109I RGGNCCY 3 cut(s) 26, 27, 99
EcoO65I GGTNACC 1 cut(s) 578
EcoRII CCWGG 2 cut(s) 441, 541
EcoT38I GRGCYC 2 cut(s) 30, 337
Esp3I CGTCTC 1 cut(s) 165
FaeI CATG 2 cut(s) 613, 636
FaiI YATR 4 cut(s) 439, 611, 629, 634
FatI CATG 2 cut(s) 609, 632
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 6 cut(s) 37, 70, 133, 375, 378, 537
FokI GGATG 1 cut(s) 377
FriOI GRGCYC 2 cut(s) 30, 337
Fsp4HI GCNGC 6 cut(s) 37, 70, 133, 375, 378, 537
GlaI GCGC 1 cut(s) 55
GluI GCNGC 6 cut(s) 37, 70, 133, 375, 378, 537
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 3 cut(s) 28, 469, 547
HapII CCGG 4 cut(s) 48, 187, 248, 330
HgaI GACGC 3 cut(s) 61, 95, 147
HhaI GCGC 1 cut(s) 56
Hin1II CATG 2 cut(s) 613, 636
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 3 cut(s) 182, 326, 503
HpaII CCGG 4 cut(s) 48, 187, 248, 330
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 3 cut(s) 13, 486, 552
Hpy188I TCNGA 6 cut(s) 85, 165, 181, 208, 291, 339
Hpy188III TCNNGA 4 cut(s) 48, 121, 409, 660
Hpy8I GTNNAC 3 cut(s) 13, 486, 552
Hpy99I CGWCG 5 cut(s) 89, 98, 146, 464, 661
HpyAV CCTTC 2 cut(s) 52, 608
HpyCH4III ACNGT 3 cut(s) 212, 392, 578
HpyCH4IV ACGT 3 cut(s) 401, 459, 651
HpyCH4V TGCA 4 cut(s) 262, 360, 452, 609
HpyF10VI GCNNNNNNNGC 4 cut(s) 60, 69, 75, 533
HpyF3I CTNAG 4 cut(s) 16, 111, 162, 517
HpySE526I ACGT 3 cut(s) 401, 459, 651
Hsp92II CATG 2 cut(s) 613, 636
HspAI GCGC 1 cut(s) 54
Kpn2I TCCGGA 1 cut(s) 47
Kzo9I GATC 1 cut(s) 165
LmnI GCTCC 3 cut(s) 44, 140, 340
Lsp1109I GCAGC 3 cut(s) 81, 361, 364
MaeII ACGT 3 cut(s) 401, 459, 651
MaeIII GTNAC 3 cut(s) 104, 314, 578
MalI GATC 1 cut(s) 167
MbiI CCGCTC 1 cut(s) 39
MboI GATC 1 cut(s) 165
MboII GAAGA 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 30, 337
MluCI AATT 4 cut(s) 151, 231, 252, 344
MmeI TCCRAC 3 cut(s) 108, 159, 269
MnlI CCTC 4 cut(s) 205, 214, 526, 599
MroI TCCGGA 1 cut(s) 47
MseI TTAA 2 cut(s) 255, 414
MspI CCGG 4 cut(s) 48, 187, 248, 330
MspR9I CCNGG 4 cut(s) 187, 331, 443, 543
MvaI CCWGG 2 cut(s) 443, 543
MwoI GCNNNNNNNGC 4 cut(s) 60, 69, 75, 533
NciI CCSGG 2 cut(s) 187, 331
NdeII GATC 1 cut(s) 165
NlaIII CATG 2 cut(s) 613, 636
NlaIV GGNNCC 5 cut(s) 28, 29, 100, 136, 336
NmuCI GTSAC 1 cut(s) 578
PasI CCCWGGG 1 cut(s) 542
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PfeI GAWTC 3 cut(s) 182, 326, 503
PfoI TCCNGGA 1 cut(s) 185
PkrI GCNGC 6 cut(s) 38, 71, 134, 376, 379, 538
Ppu21I YACGTR 1 cut(s) 402
PpuMI RGGWCCY 1 cut(s) 99
Psp1406I AACGTT 1 cut(s) 651
Psp5II RGGWCCY 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 441, 541
PspEI GGTNACC 1 cut(s) 578
PspGI CCWGG 2 cut(s) 441, 541
PspN4I GGNNCC 5 cut(s) 28, 29, 100, 136, 336
PspOMI GGGCCC 1 cut(s) 26
PspPI GGNCC 5 cut(s) 26, 27, 99, 467, 545
PspPPI RGGWCCY 1 cut(s) 99
PstNI CAGNNNCTG 1 cut(s) 75
RsaI GTAC 2 cut(s) 149, 511
RsaNI GTAC 2 cut(s) 148, 510
SalI GTCGAC 1 cut(s) 11
SaqAI TTAA 2 cut(s) 255, 414
SatI GCNGC 6 cut(s) 37, 70, 133, 375, 378, 537
Sau3AI GATC 1 cut(s) 165
Sau96I GGNCC 5 cut(s) 26, 27, 99, 467, 545
ScaI AGTACT 1 cut(s) 511
ScrFI CCNGG 4 cut(s) 187, 331, 443, 543
SduI GDGCHC 2 cut(s) 30, 337
SetI ASST 7 cut(s) 225, 261, 404, 431, 462, 529, 654
SinI GGWCC 1 cut(s) 99
Sse9I AATT 4 cut(s) 151, 231, 252, 344
SsiI CCGC 6 cut(s) 34, 37, 63, 132, 537, 583
StyD4I CCNGG 4 cut(s) 185, 329, 441, 541
TaaI ACNGT 3 cut(s) 212, 392, 578
TaiI ACGT 3 cut(s) 404, 462, 654
TaqI TCGA 5 cut(s) 12, 122, 219, 228, 497
TaqII GACCGA 1 cut(s) 205
TasI AATT 4 cut(s) 151, 231, 252, 344
TatI WGTACW 2 cut(s) 147, 509
TauI GCSGC 3 cut(s) 39, 135, 539
TfiI GAWTC 3 cut(s) 182, 326, 503
Tru1I TTAA 2 cut(s) 255, 414
Tru9I TTAA 2 cut(s) 255, 414
TseFI GTSAC 1 cut(s) 578
TseI GCWGC 3 cut(s) 69, 374, 377
Tsp45I GTSAC 1 cut(s) 578
TspGWI ACGGA 2 cut(s) 569, 645
VpaK11BI GGWCC 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.