pycom12g16640

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0018134
Physical Location & Seq
Forward (+)
18983945 .. 18984637
693 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 693 bp
ATGCAGCATAATATATTTACTACAATGCGGAGCTTGAAGATCATGGATGGGTGTAAAGGAAGTCAAGTTTTCGCGCTTAATCCTTCCGGAGCTACAGCCGCCGGCGGAAACGGCGGTGGTGGTGGTGGTGCGGGGGACAAGCTTCTTTACGACCATCTCCGGGTCAACTCGATTCGATCCAGAGCGAGTCGGGGCAGTTTTCAGGCTCCGAACCCTACCGCCAACAATGTTTTGCTCGAGACTCTTCTCCCTTACGGTCTGCCGGTGTCCGATCTCCTTGAGCCCCAAATCGAACCATCCCTCAAATCGGTCGACTTTGTCGAAACCTTAGCTGATATGTACCGTAGGATTGTGATTTGCCCCCAATTCGAGAAGTGGAAGATGTATTTGGAGCAATGTGCGACGTTTCGGGGCCTTTCGGATCCGAAATTGTTCCGGCGGAGCCTCAGGTCGGCGAGGCAGCACGCGGTTGATGTGCACTCGAAGGTGGTGCTGGCGTCTTGGTTGAGGTATGAGAGGAGAGAGGATGAGCTTATTGGGTCGTCAGCAATGGATTGTTGTGGTAGAAATGTTGAATGTCCGAAGGCTAGTTTGGTTTCCGGGTATGATCCTGAGTCTGTTTTTGAGTCCTGTATGTGTTCTAGGACGCCGCGGGGGCAAGATGACGATGATCGATCTTGTGATGAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

231

Amino Acids

25.37

Weight (kDa)

6.43

Isoelectric Point (pI)

58.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 312
AccII CGCG 3 cut(s) 74, 467, 652
AccIII TCCGGA 1 cut(s) 86
AclWI GGATC 4 cut(s) 171, 416, 429, 602
AcyI GRCGYC 2 cut(s) 497, 647
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 3 cut(s) 160, 307, 451
AgsI TTSAA 2 cut(s) 37, 575
AjuI GAANNNNNNNTTGG 4 cut(s) 371, 403, 575, 607
AluBI AGCT 5 cut(s) 33, 92, 142, 332, 532
AluI AGCT 5 cut(s) 33, 92, 142, 332, 532
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 1 cut(s) 233
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 4 cut(s) 171, 416, 429, 602
Ama87I CYCGRG 1 cut(s) 236
Aor13HI TCCGGA 1 cut(s) 86
AoxI GGCC 1 cut(s) 412
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 4, 460
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 412
AsuC2I CCSGG 2 cut(s) 161, 601
AvaI CYCGRG 1 cut(s) 236
AxyI CCTNAGG 1 cut(s) 446
BaeGI GKGCMC 1 cut(s) 480
BamHI GGATCC 1 cut(s) 421
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 16, 472
BccI CCATC 3 cut(s) 41, 162, 304
BceAI ACGGC 1 cut(s) 127
BcgI CGANNNNNNTGC 2 cut(s) 472, 506
BcnI CCSGG 2 cut(s) 161, 601
BcoDI GTCTC 1 cut(s) 233
BfaI CTAG 2 cut(s) 588, 642
BfmI CTRYAG 1 cut(s) 93
BglI GCCNNNNNGGC 1 cut(s) 655
BisI GCNGC 4 cut(s) 5, 99, 461, 650
BlsI GCNGC 4 cut(s) 6, 100, 462, 651
Bme1390I CCNGG 2 cut(s) 161, 601
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 412
BmiI GGNNCC 4 cut(s) 207, 413, 423, 443
BmrFI CCNGG 2 cut(s) 161, 601
Bpu10I CCTNAGC 1 cut(s) 328
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 2 cut(s) 161, 601
Bsa29I ATCGAT 1 cut(s) 673
BsaHI GRCGYC 2 cut(s) 497, 647
BsaJI CCNNGG 1 cut(s) 650
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 3 cut(s) 160, 307, 451
Bse118I RCCGGY 2 cut(s) 101, 262
Bse21I CCTNAGG 1 cut(s) 446
Bse3DI GCAATG 2 cut(s) 401, 555
BseAI TCCGGA 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 673
BseDI CCNNGG 1 cut(s) 650
BseGI GGATG 3 cut(s) 52, 296, 532
BseLI CCNNNNNNNGG 3 cut(s) 160, 307, 451
BseMI GCAATG 2 cut(s) 401, 555
BseMII CTCAG 2 cut(s) 460, 603
BseRI GAGGAG 1 cut(s) 532
BseSI GKGCMC 1 cut(s) 480
BseXI GCAGC 2 cut(s) 16, 472
Bsh1236I CGCG 3 cut(s) 74, 467, 652
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 1 cut(s) 414
BshVI ATCGAT 1 cut(s) 673
BsiEI CGRYCG 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 480
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 6 cut(s) 87, 102, 160, 263, 436, 600
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 3 cut(s) 160, 307, 451
BsmAI GTCTC 1 cut(s) 233
BsmFI GGGAC 1 cut(s) 149
BsnI GGCC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 285, 480
Bsp13I TCCGGA 1 cut(s) 86
Bsp143I GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
BspANI GGCC 1 cut(s) 414
BspCNI CTCAG 2 cut(s) 459, 604
BspDI ATCGAT 1 cut(s) 673
BspEI TCCGGA 1 cut(s) 86
BspFNI CGCG 3 cut(s) 74, 467, 652
BspLI GGNNCC 4 cut(s) 207, 413, 423, 443
BspPI GGATC 4 cut(s) 171, 416, 429, 602
BsrDI GCAATG 2 cut(s) 401, 555
BsrFI RCCGGY 2 cut(s) 101, 262
BssAI RCCGGY 2 cut(s) 101, 262
BssECI CCNNGG 1 cut(s) 650
BssMI GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
BssNI GRCGYC 2 cut(s) 497, 647
Bst4CI ACNGT 2 cut(s) 257, 344
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 2 cut(s) 497, 647
BstC8I GCNNGC 3 cut(s) 103, 465, 495
BstDEI CTNAG 3 cut(s) 328, 446, 612
BstDSI CCRYGG 1 cut(s) 650
BstF5I GGATG 3 cut(s) 52, 296, 532
BstFNI CGCG 3 cut(s) 74, 467, 652
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 7 cut(s) 42, 179, 274, 424, 610, 673, 677
BstMAI GTCTC 1 cut(s) 233
BstMBI GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 3 cut(s) 98, 111, 655
BstSCI CCNGG 2 cut(s) 159, 599
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 480
BstUI CGCG 3 cut(s) 74, 467, 652
BstV1I GCAGC 2 cut(s) 16, 472
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
Bsu15I ATCGAT 1 cut(s) 673
Bsu36I CCTNAGG 1 cut(s) 446
BsuRI GGCC 1 cut(s) 414
BsuTUI ATCGAT 1 cut(s) 673
BtgI CCRYGG 1 cut(s) 650
BtsCI GGATG 3 cut(s) 52, 296, 532
Cac8I GCNNGC 3 cut(s) 103, 465, 495
CfoI GCGC 1 cut(s) 76
Cfr10I RCCGGY 2 cut(s) 101, 262
Cfr13I GGNCC 1 cut(s) 412
Cfr42I CCGCGG 1 cut(s) 653
ClaI ATCGAT 1 cut(s) 673
CseI GACGC 2 cut(s) 486, 655
Csp6I GTAC 1 cut(s) 340
CviAII CATG 1 cut(s) 43
CviQI GTAC 1 cut(s) 340
DdeI CTNAG 3 cut(s) 328, 446, 612
DpnI GATC 7 cut(s) 41, 178, 273, 423, 609, 672, 676
DpnII GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
EciI GGCGGA 2 cut(s) 120, 454
Eco24I GRGCYC 1 cut(s) 285
Eco81I CCTNAGG 1 cut(s) 446
Eco88I CYCGRG 1 cut(s) 236
EcoO109I RGGNCCY 1 cut(s) 412
EcoT38I GRGCYC 1 cut(s) 285
FaeI CATG 1 cut(s) 46
FaiI YATR 7 cut(s) 9, 14, 44, 338, 513, 606, 635
FaqI GGGAC 1 cut(s) 149
FatI CATG 1 cut(s) 42
FauI CCCGC 2 cut(s) 124, 645
FblI GTMKAC 1 cut(s) 312
Fnu4HI GCNGC 4 cut(s) 5, 99, 461, 650
FokI GGATG 3 cut(s) 59, 283, 539
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 4 cut(s) 5, 99, 461, 650
FspBI CTAG 2 cut(s) 588, 642
GlaI GCGC 1 cut(s) 75
GluI GCNGC 4 cut(s) 5, 99, 461, 650
HaeIII GGCC 1 cut(s) 414
HapII CCGG 6 cut(s) 87, 102, 160, 263, 436, 600
HgaI GACGC 2 cut(s) 486, 655
HhaI GCGC 1 cut(s) 76
Hin1I GRCGYC 2 cut(s) 497, 647
Hin1II CATG 1 cut(s) 46
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 2 cut(s) 166, 313
HindII GTYRAC 2 cut(s) 166, 313
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 5 cut(s) 172, 187, 241, 614, 626
HpaII CCGG 6 cut(s) 87, 102, 160, 263, 436, 600
Hpy166II GTNNAC 3 cut(s) 166, 313, 478
Hpy188I TCNGA 5 cut(s) 210, 271, 421, 426, 582
Hpy188III TCNNGA 5 cut(s) 87, 180, 238, 370, 611
Hpy8I GTNNAC 3 cut(s) 166, 313, 478
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 3 cut(s) 93, 478, 577
HpyCH4III ACNGT 2 cut(s) 257, 344
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 2 cut(s) 4, 478
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 111, 655
HpyF3I CTNAG 3 cut(s) 328, 446, 612
HpySE526I ACGT 1 cut(s) 404
Hsp92I GRCGYC 2 cut(s) 497, 647
Hsp92II CATG 1 cut(s) 46
HspAI GCGC 1 cut(s) 74
Kpn2I TCCGGA 1 cut(s) 86
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
KspI CCGCGG 1 cut(s) 653
Kzo9I GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
LmnI GCTCC 5 cut(s) 30, 89, 211, 391, 441
Lsp1109I GCAGC 2 cut(s) 16, 472
MaeI CTAG 2 cut(s) 588, 642
MaeII ACGT 1 cut(s) 404
MalI GATC 7 cut(s) 41, 178, 273, 423, 609, 672, 676
MboI GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
MboII GAAGA 3 cut(s) 49, 236, 391
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 285, 480
MluCI AATT 2 cut(s) 365, 428
MlyI GAGTC 4 cut(s) 196, 235, 623, 635
MnlI CCTC 7 cut(s) 311, 450, 455, 501, 510, 517, 679
MreI CGCCGGCG 1 cut(s) 101
MroI TCCGGA 1 cut(s) 86
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 652
MspI CCGG 6 cut(s) 87, 102, 160, 263, 436, 600
MspR9I CCNGG 2 cut(s) 161, 601
MvnI CGCG 3 cut(s) 74, 467, 652
MwoI GCNNNNNNNGC 3 cut(s) 98, 111, 655
NaeI GCCGGC 1 cut(s) 103
NciI CCSGG 2 cut(s) 161, 601
NdeII GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 1 cut(s) 46
NlaIV GGNNCC 4 cut(s) 207, 413, 423, 443
PaeR7I CTCGAG 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 431
PfeI GAWTC 1 cut(s) 172
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 4 cut(s) 6, 100, 462, 651
PleI GAGTC 4 cut(s) 195, 235, 622, 634
PpsI GAGTC 4 cut(s) 195, 235, 622, 634
PspN4I GGNNCC 4 cut(s) 207, 413, 423, 443
PspPI GGNCC 1 cut(s) 412
PsuI RGATCY 1 cut(s) 421
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SacII CCGCGG 1 cut(s) 653
SalI GTCGAC 1 cut(s) 311
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 4 cut(s) 5, 99, 461, 650
Sau3AI GATC 7 cut(s) 39, 176, 271, 421, 607, 670, 674
Sau96I GGNCC 1 cut(s) 412
SchI GAGTC 4 cut(s) 196, 235, 623, 635
ScrFI CCNGG 2 cut(s) 161, 601
SduI GDGCHC 2 cut(s) 285, 480
SfcI CTRYAG 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 236
Sfr303I CCGCGG 1 cut(s) 653
SgrAI CRCCGGYG 1 cut(s) 101
SgrBI CCGCGG 1 cut(s) 653
SlaI CTCGAG 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 236, 278
SmoI CTYRAG 2 cut(s) 236, 278
Sse9I AATT 2 cut(s) 365, 428
SspMI CTAG 2 cut(s) 588, 642
StyD4I CCNGG 2 cut(s) 159, 599
TaaI ACNGT 2 cut(s) 257, 344
TaiI ACGT 1 cut(s) 407
TaqI TCGA 9 cut(s) 170, 175, 237, 291, 312, 321, 369, 482, 673
TaqII GACCGA 1 cut(s) 298
TasI AATT 2 cut(s) 365, 428
TauI GCSGC 2 cut(s) 101, 652
TfiI GAWTC 1 cut(s) 172
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TseI GCWGC 2 cut(s) 4, 460
Tth111I GACNNNGTC 1 cut(s) 317
VneI GTGCAC 1 cut(s) 476
XhoI CTCGAG 1 cut(s) 236
XmiI GTMKAC 1 cut(s) 312
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 2 cut(s) 588, 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.