RLG00000007193

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018134
Physical Location & Seq
Reverse (-)
14445385 .. 14447468
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007193

Sequence Viewer

Length: 798 bp
ATGAAGATTGATGGTAGTGATCATGATGTCCTCTGCACCCGCGTCGCACCCAATTACTCCCCCCTCCTCCACCACCACCGTCGCCATTGGAAAAAGACCCATAAGAATCTGAGACCAAGTGTGTTGATGCTAGCTGTAAATATAGTGTTGTTCTTCTTCTCTGCTTGTTTTCCGGGTGCTCAGACGGTGGTGCTTGCTGGGCATGAGGCTGTGAATTTATTCAAGTTGAAGATGCAGCACAATATATTTACTACAATGCGCAGCTGGAAGATCATGAATGGGTGTAAGGGCACCCAGGCTTTTACCATTAATCCATCTGGAACAACCCCTACAAATGGTGGAGTAGGAGACAAGGTTGGTCACAGCCGGGTCAACTCGGTTCGATCTAGATCGAACAGGAGTTTTCAAGCCCCGAACCCGACCAGAAACAATGCCTTGGTCGAGAGTCTTATTCCCTATGAACTCCCATCGTTAGATCTCCTTGAGCCCCAAATCGAGCCTTGCCTCAAGACTGTTGATTTTGTCGAAACATTGGCTGATGTGTACAGCAGGATTGAGAATTTTCTGCAATTCGAGAAATCTAAGATGTACGTGGAGCAATGCGCAACCTTTCGGGGTACGTCGGATCAGAAACTGTTCTGGCGGAGTCTCCAGTCGGCGAGGCAGCACGCGGTGGATGTCCATACCAAAGTGGTACTTGAGGCGTGGTTCAGGTATGAGAGGAGGGAGGATGAGCTGGTTGGGTCATCTTCAATGACTTGTTGCAGTAGAAATGTTGAACCAAGGCTAGTTTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

266

Amino Acids

30.26

Weight (kDa)

9.32

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 260, 604
AccB1I GGYRCC 1 cut(s) 290
AccII CGCG 2 cut(s) 42, 671
AciI CCGC 3 cut(s) 40, 643, 671
AclWI GGATC 1 cut(s) 633
AcsI RAATTY 2 cut(s) 214, 559
AdeI CACNNNGTG 1 cut(s) 673
AfaI GTAC 4 cut(s) 545, 590, 619, 696
AfiI CCNNNNNNNGG 1 cut(s) 335
AgsI TTSAA 5 cut(s) 223, 229, 407, 753, 779
AjnI CCWGG 1 cut(s) 294
AluBI AGCT 3 cut(s) 134, 264, 736
AluI AGCT 3 cut(s) 134, 264, 736
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 3 cut(s) 106, 342, 653
AlwI GGATC 1 cut(s) 633
AlwNI CAGNNNCTG 1 cut(s) 634
ApeKI GCWGC 3 cut(s) 235, 261, 664
ApoI RAATTY 2 cut(s) 214, 559
AseI ATTAAT 1 cut(s) 309
Asp700I GAANNNNTTC 2 cut(s) 218, 635
AspLEI GCGC 2 cut(s) 261, 605
AsuC2I CCSGG 2 cut(s) 174, 368
AsuNHI GCTAGC 1 cut(s) 130
BaeGI GKGCMC 1 cut(s) 293
BanI GGYRCC 1 cut(s) 290
BanII GRGCYC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 181
BbvI GCAGC 3 cut(s) 247, 273, 676
BccI CCATC 3 cut(s) 5, 322, 475
BcgI CGANNNNNNTGC 2 cut(s) 25, 59
BciT130I CCWGG 1 cut(s) 296
BclI TGATCA 1 cut(s) 19
BcnI CCSGG 2 cut(s) 174, 368
BcoDI GTCTC 3 cut(s) 106, 342, 653
BfaI CTAG 3 cut(s) 131, 387, 788
BglII AGATCT 1 cut(s) 475
BisI GCNGC 3 cut(s) 236, 262, 665
BlsI GCNGC 3 cut(s) 237, 263, 666
Bme1390I CCNGG 3 cut(s) 174, 296, 368
BmiI GGNNCC 1 cut(s) 292
BmrFI CCNGG 3 cut(s) 174, 296, 368
BmsI GCATC 2 cut(s) 117, 222
BmtI GCTAGC 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 635
BpuEI CTTGAG 3 cut(s) 491, 503, 719
BpuMI CCSGG 2 cut(s) 174, 368
BsaAI YACGTR 1 cut(s) 592
BsaBI GATNNNNATC 1 cut(s) 388
BsaI GGTCTC 1 cut(s) 106
BsaJI CCNNGG 3 cut(s) 294, 435, 782
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse1I ACTGG 1 cut(s) 652
Bse3DI GCAATG 1 cut(s) 605
Bse8I GATNNNNATC 1 cut(s) 388
BseBI CCWGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 294, 435, 782
BseGI GGATG 2 cut(s) 682, 736
BseJI GATNNNNATC 1 cut(s) 388
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMI GCAATG 1 cut(s) 605
BseMII CTCAG 2 cut(s) 101, 194
BseNI ACTGG 1 cut(s) 652
BseRI GAGGAG 2 cut(s) 56, 736
BseSI GKGCMC 1 cut(s) 293
BseXI GCAGC 3 cut(s) 247, 273, 676
BseYI CCCAGC 1 cut(s) 197
BsgI GTGCAG 1 cut(s) 19
Bsh1236I CGCG 2 cut(s) 42, 671
BshNI GGYRCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 181
BsiSI CCGG 2 cut(s) 173, 367
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 3 cut(s) 106, 342, 653
Bso31I GGTCTC 1 cut(s) 106
Bsp1286I GDGCHC 3 cut(s) 181, 293, 489
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 6 cut(s) 19, 270, 383, 389, 475, 625
BspACI CCGC 3 cut(s) 40, 643, 671
BspCNI CTCAG 2 cut(s) 102, 193
BspFNI CGCG 2 cut(s) 42, 671
BspHI TCATGA 2 cut(s) 22, 273
BspLI GGNNCC 1 cut(s) 292
BspOI GCTAGC 1 cut(s) 134
BspPI GGATC 1 cut(s) 633
BspT107I GGYRCC 1 cut(s) 290
BspTNI GGTCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 605
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 3 cut(s) 294, 435, 782
BssMI GATC 6 cut(s) 19, 270, 383, 389, 475, 625
BssT1I CCWWGG 2 cut(s) 435, 782
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 4 cut(s) 80, 187, 514, 636
BstAUI TGTACA 1 cut(s) 543
BstBAI YACGTR 1 cut(s) 592
BstC8I GCNNGC 3 cut(s) 132, 195, 669
BstDEI CTNAG 3 cut(s) 110, 180, 582
BstF5I GGATG 2 cut(s) 682, 736
BstFNI CGCG 2 cut(s) 42, 671
BstHHI GCGC 2 cut(s) 261, 605
BstKTI GATC 6 cut(s) 22, 273, 386, 392, 478, 628
BstMAI GTCTC 3 cut(s) 106, 342, 653
BstMBI GATC 6 cut(s) 19, 270, 383, 389, 475, 625
BstMWI GCNNNNNNNGC 1 cut(s) 199
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 3 cut(s) 172, 294, 366
BstSLI GKGCMC 1 cut(s) 293
BstUI CGCG 2 cut(s) 42, 671
BstV1I GCAGC 3 cut(s) 247, 273, 676
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
BtsCI GGATG 2 cut(s) 682, 736
Cac8I GCNNGC 3 cut(s) 132, 195, 669
CaiI CAGNNNCTG 1 cut(s) 634
CciI TCATGA 2 cut(s) 22, 273
CfoI GCGC 2 cut(s) 261, 605
CseI GACGC 1 cut(s) 31
Csp6I GTAC 4 cut(s) 544, 589, 618, 695
CviAII CATG 3 cut(s) 23, 203, 274
CviQI GTAC 4 cut(s) 544, 589, 618, 695
DdeI CTNAG 3 cut(s) 110, 180, 582
DpnI GATC 6 cut(s) 21, 272, 385, 391, 477, 627
DpnII GATC 6 cut(s) 19, 270, 383, 389, 475, 625
DraIII CACNNNGTG 1 cut(s) 673
EciI GGCGGA 1 cut(s) 658
Eco130I CCWWGG 2 cut(s) 435, 782
Eco24I GRGCYC 1 cut(s) 489
Eco31I GGTCTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 294
EcoT14I CCWWGG 2 cut(s) 435, 782
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 2 cut(s) 435, 782
FaeI CATG 3 cut(s) 26, 206, 277
FaiI YATR 9 cut(s) 24, 102, 143, 204, 245, 275, 459, 684, 717
FalI AAGNNNNNCTT 2 cut(s) 681, 713
FatI CATG 3 cut(s) 22, 202, 273
FauI CCCGC 1 cut(s) 47
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 3 cut(s) 236, 262, 665
FokI GGATG 2 cut(s) 689, 743
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 3 cut(s) 236, 262, 665
FspBI CTAG 3 cut(s) 131, 387, 788
FspI TGCGCA 2 cut(s) 260, 604
GlaI GCGC 2 cut(s) 260, 604
GluI GCNGC 3 cut(s) 236, 262, 665
GsaI CCCAGC 1 cut(s) 201
GsuI CTGGAG 1 cut(s) 635
HapII CCGG 2 cut(s) 173, 367
HgaI GACGC 1 cut(s) 31
HhaI GCGC 2 cut(s) 261, 605
Hin1II CATG 3 cut(s) 26, 206, 277
Hin6I GCGC 2 cut(s) 259, 603
HinP1I GCGC 2 cut(s) 259, 603
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HinfI GANTC 3 cut(s) 106, 445, 646
HpaII CCGG 2 cut(s) 173, 367
Hpy166II GTNNAC 2 cut(s) 373, 544
Hpy188I TCNGA 4 cut(s) 111, 183, 625, 630
Hpy188III TCNNGA 7 cut(s) 23, 274, 318, 387, 442, 508, 574
Hpy8I GTNNAC 2 cut(s) 373, 544
Hpy99I CGWCG 3 cut(s) 47, 84, 625
HpyCH4III ACNGT 4 cut(s) 80, 187, 514, 636
HpyCH4IV ACGT 2 cut(s) 591, 620
HpyCH4V TGCA 4 cut(s) 36, 235, 568, 765
HpyF10VI GCNNNNNNNGC 1 cut(s) 199
HpyF3I CTNAG 3 cut(s) 110, 180, 582
HpySE526I ACGT 2 cut(s) 591, 620
Hsp92II CATG 3 cut(s) 26, 206, 277
HspAI GCGC 2 cut(s) 259, 603
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 6 cut(s) 19, 270, 383, 389, 475, 625
LmnI GCTCC 1 cut(s) 595
Lsp1109I GCAGC 3 cut(s) 247, 273, 676
LweI GCATC 2 cut(s) 117, 222
MaeI CTAG 3 cut(s) 131, 387, 788
MaeII ACGT 2 cut(s) 591, 620
MaeIII GTNAC 1 cut(s) 359
MalI GATC 6 cut(s) 21, 272, 385, 391, 477, 627
MboI GATC 6 cut(s) 19, 270, 383, 389, 475, 625
MboII GAAGA 6 cut(s) 16, 145, 148, 241, 280, 741
MflI RGATCY 1 cut(s) 475
MhlI GDGCHC 3 cut(s) 181, 293, 489
MluCI AATT 4 cut(s) 52, 214, 559, 569
MlyI GAGTC 2 cut(s) 454, 655
MmeI TCCRAC 1 cut(s) 603
MroXI GAANNNNTTC 2 cut(s) 218, 635
MseI TTAA 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 2 cut(s) 173, 367
MspR9I CCNGG 3 cut(s) 174, 296, 368
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 2 cut(s) 42, 671
MwoI GCNNNNNNNGC 1 cut(s) 199
NciI CCSGG 2 cut(s) 174, 368
NdeII GATC 6 cut(s) 19, 270, 383, 389, 475, 625
NheI GCTAGC 1 cut(s) 130
NlaIII CATG 3 cut(s) 26, 206, 277
NlaIV GGNNCC 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 359
NsbI TGCGCA 2 cut(s) 260, 604
PagI TCATGA 2 cut(s) 22, 273
PdmI GAANNNNTTC 2 cut(s) 218, 635
PfeI GAWTC 1 cut(s) 106
PkrI GCNGC 3 cut(s) 237, 263, 666
PleI GAGTC 2 cut(s) 453, 654
PpsI GAGTC 2 cut(s) 453, 654
Ppu21I YACGTR 1 cut(s) 592
PshBI ATTAAT 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 294
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 292
PsrI GAACNNNNNNTAC 2 cut(s) 313, 345
PstNI CAGNNNCTG 1 cut(s) 634
PsuI RGATCY 1 cut(s) 475
PvuII CAGCTG 1 cut(s) 264
RsaI GTAC 4 cut(s) 545, 590, 619, 696
RsaNI GTAC 4 cut(s) 544, 589, 618, 695
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 3 cut(s) 236, 262, 665
Sau3AI GATC 6 cut(s) 19, 270, 383, 389, 475, 625
SchI GAGTC 2 cut(s) 454, 655
ScrFI CCNGG 3 cut(s) 174, 296, 368
SduI GDGCHC 3 cut(s) 181, 293, 489
SetI ASST 8 cut(s) 136, 266, 357, 594, 611, 623, 716, 738
SfaNI GCATC 2 cut(s) 117, 222
SmlI CTYRAG 3 cut(s) 482, 506, 698
SmoI CTYRAG 3 cut(s) 482, 506, 698
Sse9I AATT 4 cut(s) 52, 214, 559, 569
SsiI CCGC 3 cut(s) 40, 643, 671
SspMI CTAG 3 cut(s) 131, 387, 788
StyD4I CCNGG 3 cut(s) 172, 294, 366
StyI CCWWGG 2 cut(s) 435, 782
TaaI ACNGT 4 cut(s) 80, 187, 514, 636
TaiI ACGT 2 cut(s) 594, 623
TaqI TCGA 6 cut(s) 382, 392, 441, 495, 525, 573
TasI AATT 4 cut(s) 52, 214, 559, 569
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 1 cut(s) 106
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 3 cut(s) 235, 261, 664
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 3 cut(s) 17, 290, 474
VspI ATTAAT 1 cut(s) 309
XapI RAATTY 2 cut(s) 214, 559
XbaI TCTAGA 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 789
XmnI GAANNNNTTC 2 cut(s) 218, 635
XspI CTAG 3 cut(s) 131, 387, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.