RLG00000024870

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018134
Physical Location & Seq
Forward (+)
39928181 .. 39929127
947 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024870

Sequence Viewer

Length: 873 bp
ATGCAGCACAATATATTTACTACAATGCGTAGCTTGAAGATCATGGATGGGTGTAAGGGCACCCAGGTGTTCGCCATTAATCCATCTGGAACAACCCCCACAAATGGTGGAGTAGGAGACAAGGTTGGTCACAGCCGGGTCAACTCGGTTCGATCCAGATCGAACTGGAGTTTCCAAGCCCCGAACCCGACCGGAAACAATGCCTTGGTCGAGAGTCTTCTTCCCTATGAACTCCCATCGTCGGATCTCCTCGAGCCCCAAATCGAGCCTTGCCTCAAGACCGTTGATTTCGTCGAGACGCTGGCTGATGTGTACCGCAGGATTGAGAATTGCCCGCAATTCGAGAAATCTAAGATGTACGTGGAGCAATGCGCGATCTTTCGGGGTGTGTCGGATCCGAAACTGTTCCGGCGGAGTCTCCGGTCGGCGAGGCAGCACGCGGTGGACGTCCACACGAAGGTGGTGCTTGCGGCGTGGTTGAGGTACGAGAGGAGGGAGGATGAGCTGGTTGGGTCATCTTCGATGACTTGTTGCGGTAGAAATGTTGAATGTCCCAAGGCTAGTTTGGTAGCTGGGTATGATCCGGAGTCTGTTTATGATTCATGTTGCTGCTCTCGGAGCGGCGGCGATGTGGACGGTGAGACTGCGGATGCGGATGAAGAATGCTCGACTTCGAAGGAGGAAGAAGCTGATATGTCATTTTACATTGGAGAGGATGAGATAATGTGTGTTAGGTATAAGATTGCATCGCTCTCGACACCCTTTAAGACAATGTTGTATGGGGGCTTTACGGAGACGAGGAGGCAGAAGATAAATTTCTCACAGAATGGAGTTTCTCCGGAGGCAATGAGAGCAGTGGAGGTTTTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

291

Amino Acids

32.25

Weight (kDa)

5.31

Isoelectric Point (pI)

54.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 450
AccB1I GGYRCC 1 cut(s) 59
AccBSI CCGCTC 1 cut(s) 621
AccII CGCG 2 cut(s) 374, 440
AccIII TCCGGA 2 cut(s) 583, 838
AclWI GGATC 5 cut(s) 147, 252, 389, 402, 575
AcsI RAATTY 1 cut(s) 814
AcyI GRCGYC 1 cut(s) 447
AdeI CACNNNGTG 1 cut(s) 442
AfaI GTAC 3 cut(s) 314, 359, 485
AfiI CCNNNNNNNGG 3 cut(s) 104, 241, 457
AgsI TTSAA 2 cut(s) 37, 548
AjnI CCWGG 1 cut(s) 63
AleI CACNNNNGTG 2 cut(s) 65, 458
AluBI AGCT 4 cut(s) 33, 505, 572, 689
AluI AGCT 4 cut(s) 33, 505, 572, 689
Alw26I GTCTC 5 cut(s) 111, 290, 422, 635, 788
AlwI GGATC 5 cut(s) 147, 252, 389, 402, 575
Ama87I CYCGRG 1 cut(s) 251
Aor13HI TCCGGA 2 cut(s) 583, 838
ApeKI GCWGC 3 cut(s) 4, 433, 609
ApoI RAATTY 1 cut(s) 814
AseI ATTAAT 1 cut(s) 78
Asp700I GAANNNNTTC 1 cut(s) 404
AspLEI GCGC 1 cut(s) 374
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 1 cut(s) 650
AsuII TTCGAA 1 cut(s) 674
AvaI CYCGRG 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 62
BamHI GGATCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 209
BbvI GCAGC 3 cut(s) 16, 445, 596
BccI CCATC 3 cut(s) 41, 91, 244
BcgI CGANNNNNNTGC 4 cut(s) 445, 479, 735, 769
BciT130I CCWGG 1 cut(s) 65
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 5 cut(s) 111, 290, 422, 635, 788
BfaI CTAG 1 cut(s) 561
BisI GCNGC 6 cut(s) 5, 434, 471, 610, 622, 625
BlsI GCNGC 6 cut(s) 6, 435, 472, 611, 623, 626
Bme1390I CCNGG 2 cut(s) 65, 137
BmeT110I CYCGRG 1 cut(s) 251
BmiI GGNNCC 2 cut(s) 61, 396
BmrFI CCNGG 2 cut(s) 65, 137
BmsI GCATC 2 cut(s) 640, 755
BpiI GAAGAC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 187
Bpu14I TTCGAA 1 cut(s) 674
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 137
BsaAI YACGTR 1 cut(s) 361
BsaBI GATNNNNATC 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 447
BsaJI CCNNGG 3 cut(s) 63, 204, 555
BsaWI WCCGGW 4 cut(s) 191, 420, 583, 838
BsaXI ACNNNNNCTCC 3 cut(s) 108, 138, 851
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 241, 457
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 2 cut(s) 374, 852
Bse8I GATNNNNATC 1 cut(s) 157
BseAI TCCGGA 2 cut(s) 583, 838
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 3 cut(s) 63, 204, 555
BseGI GGATG 5 cut(s) 52, 505, 655, 661, 721
BseJI GATNNNNATC 1 cut(s) 157
BseLI CCNNNNNNNGG 3 cut(s) 104, 241, 457
BseMI GCAATG 2 cut(s) 374, 852
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 3 cut(s) 239, 505, 814
BseSI GKGCMC 1 cut(s) 62
BseXI GCAGC 3 cut(s) 16, 445, 596
BseYI CCCAGC 1 cut(s) 572
Bsh1236I CGCG 2 cut(s) 374, 440
Bsh1285I CGRYCG 2 cut(s) 192, 425
BshNI GGYRCC 1 cut(s) 59
BsiEI CGRYCG 2 cut(s) 192, 425
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 6 cut(s) 136, 192, 409, 421, 584, 839
BslFI GGGAC 1 cut(s) 537
BslI CCNNNNNNNGG 3 cut(s) 104, 241, 457
BsmAI GTCTC 5 cut(s) 111, 290, 422, 635, 788
BsmBI CGTCTC 2 cut(s) 290, 788
BsmFI GGGAC 1 cut(s) 537
BsmI GAATGC 1 cut(s) 668
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 674
Bsp1286I GDGCHC 2 cut(s) 62, 258
Bsp13I TCCGGA 2 cut(s) 583, 838
Bsp143I GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
BspEI TCCGGA 2 cut(s) 583, 838
BspFNI CGCG 2 cut(s) 374, 440
BspLI GGNNCC 2 cut(s) 61, 396
BspPI GGATC 5 cut(s) 147, 252, 389, 402, 575
BspT104I TTCGAA 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 59
BsrBI CCGCTC 1 cut(s) 621
BsrDI GCAATG 2 cut(s) 374, 852
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 63, 204, 555
BssMI GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
BssNI GRCGYC 1 cut(s) 447
BssT1I CCWWGG 2 cut(s) 204, 555
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 3 cut(s) 283, 405, 638
BstACI GRCGYC 1 cut(s) 447
BstBAI YACGTR 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 303, 335, 438, 468
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 5 cut(s) 52, 505, 655, 661, 721
BstFNI CGCG 2 cut(s) 374, 440
BstHHI GCGC 1 cut(s) 374
BstKTI GATC 7 cut(s) 42, 155, 161, 247, 378, 397, 583
BstMAI GTCTC 5 cut(s) 111, 290, 422, 635, 788
BstMBI GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
BstMCI CGRYCG 2 cut(s) 192, 425
BstMWI GCNNNNNNNGC 2 cut(s) 618, 851
BstNI CCWGG 1 cut(s) 65
BstSCI CCNGG 2 cut(s) 63, 135
BstSLI GKGCMC 1 cut(s) 62
BstUI CGCG 2 cut(s) 374, 440
BstV1I GCAGC 3 cut(s) 16, 445, 596
BstV2I GAAGAC 1 cut(s) 209
BstX2I RGATCY 2 cut(s) 244, 394
BstYI RGATCY 2 cut(s) 244, 394
BtgZI GCGATG 2 cut(s) 642, 732
BtsCI GGATG 5 cut(s) 52, 505, 655, 661, 721
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 1 cut(s) 861
Cac8I GCNNGC 4 cut(s) 303, 335, 438, 468
CfoI GCGC 1 cut(s) 374
CseI GACGC 1 cut(s) 307
Csp6I GTAC 3 cut(s) 313, 358, 484
CviAII CATG 2 cut(s) 43, 603
CviQI GTAC 3 cut(s) 313, 358, 484
DdeI CTNAG 1 cut(s) 351
DpnI GATC 7 cut(s) 41, 154, 160, 246, 377, 396, 582
DpnII GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
DraIII CACNNNGTG 1 cut(s) 442
EciI GGCGGA 1 cut(s) 427
Eco130I CCWWGG 2 cut(s) 204, 555
Eco24I GRGCYC 1 cut(s) 258
Eco88I CYCGRG 1 cut(s) 251
EcoRII CCWGG 1 cut(s) 63
EcoT14I CCWWGG 2 cut(s) 204, 555
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 2 cut(s) 204, 555
Esp3I CGTCTC 2 cut(s) 290, 788
FaeI CATG 2 cut(s) 46, 606
FaiI YATR 9 cut(s) 14, 44, 228, 579, 597, 604, 695, 738, 780
FaqI GGGAC 1 cut(s) 537
FatI CATG 2 cut(s) 42, 602
FauI CCCGC 1 cut(s) 342
Fnu4HI GCNGC 6 cut(s) 5, 434, 471, 610, 622, 625
FokI GGATG 5 cut(s) 59, 512, 662, 668, 728
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 6 cut(s) 5, 434, 471, 610, 622, 625
FspBI CTAG 1 cut(s) 561
GlaI GCGC 1 cut(s) 373
GluI GCNGC 6 cut(s) 5, 434, 471, 610, 622, 625
GsaI CCCAGC 1 cut(s) 576
GsuI CTGGAG 1 cut(s) 187
HapII CCGG 6 cut(s) 136, 192, 409, 421, 584, 839
HgaI GACGC 1 cut(s) 307
HhaI GCGC 1 cut(s) 374
Hin1I GRCGYC 1 cut(s) 447
Hin1II CATG 2 cut(s) 46, 606
Hin6I GCGC 1 cut(s) 372
HinP1I GCGC 1 cut(s) 372
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 4 cut(s) 214, 415, 587, 599
HpaII CCGG 6 cut(s) 136, 192, 409, 421, 584, 839
HphI GGTGA 1 cut(s) 650
Hpy166II GTNNAC 5 cut(s) 142, 313, 445, 451, 634
Hpy188I TCNGA 4 cut(s) 244, 394, 399, 618
Hpy188III TCNNGA 9 cut(s) 87, 156, 211, 277, 295, 343, 584, 754, 839
Hpy8I GTNNAC 5 cut(s) 142, 313, 445, 451, 634
Hpy99I CGWCG 2 cut(s) 244, 296
HpyAV CCTTC 2 cut(s) 451, 670
HpyCH4III ACNGT 3 cut(s) 283, 405, 638
HpyCH4IV ACGT 2 cut(s) 360, 447
HpyCH4V TGCA 2 cut(s) 4, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 618, 851
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 2 cut(s) 360, 447
Hsp92I GRCGYC 1 cut(s) 447
Hsp92II CATG 2 cut(s) 46, 606
HspAI GCGC 1 cut(s) 372
Kpn2I TCCGGA 2 cut(s) 583, 838
Kzo9I GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
LmnI GCTCC 2 cut(s) 364, 618
Lsp1109I GCAGC 3 cut(s) 16, 445, 596
LweI GCATC 2 cut(s) 640, 755
MaeI CTAG 1 cut(s) 561
MaeII ACGT 2 cut(s) 360, 447
MaeIII GTNAC 1 cut(s) 128
MalI GATC 7 cut(s) 41, 154, 160, 246, 377, 396, 582
MbiI CCGCTC 1 cut(s) 621
MboI GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
MboII GAAGA 7 cut(s) 49, 209, 212, 510, 671, 695, 820
MflI RGATCY 2 cut(s) 244, 394
MhlI GDGCHC 2 cut(s) 62, 258
MluCI AATT 3 cut(s) 328, 338, 814
MlyI GAGTC 3 cut(s) 223, 424, 596
MmeI TCCRAC 2 cut(s) 222, 372
MroI TCCGGA 2 cut(s) 583, 838
MroXI GAANNNNTTC 1 cut(s) 404
MseI TTAA 2 cut(s) 78, 765
MslI CAYNNNNRTG 2 cut(s) 65, 458
MspI CCGG 6 cut(s) 136, 192, 409, 421, 584, 839
MspR9I CCNGG 2 cut(s) 65, 137
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 2 cut(s) 374, 440
MwoI GCNNNNNNNGC 2 cut(s) 618, 851
NciI CCSGG 1 cut(s) 137
NdeII GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
NlaIII CATG 2 cut(s) 46, 606
NlaIV GGNNCC 2 cut(s) 61, 396
NmuCI GTSAC 1 cut(s) 128
NspV TTCGAA 1 cut(s) 674
OliI CACNNNNGTG 2 cut(s) 65, 458
PaeR7I CTCGAG 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PctI GAATGC 1 cut(s) 668
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 1 cut(s) 599
PkrI GCNGC 6 cut(s) 6, 435, 472, 611, 623, 626
PleI GAGTC 3 cut(s) 222, 423, 595
PpsI GAGTC 3 cut(s) 222, 423, 595
Ppu21I YACGTR 1 cut(s) 361
PshBI ATTAAT 1 cut(s) 78
Psp6I CCWGG 1 cut(s) 63
PspFI CCCAGC 1 cut(s) 572
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 2 cut(s) 61, 396
PspXI VCTCGAGB 1 cut(s) 251
PsuI RGATCY 2 cut(s) 244, 394
RsaI GTAC 3 cut(s) 314, 359, 485
RsaNI GTAC 3 cut(s) 313, 358, 484
RseI CAYNNNNRTG 2 cut(s) 65, 458
SaqAI TTAA 2 cut(s) 78, 765
SatI GCNGC 6 cut(s) 5, 434, 471, 610, 622, 625
Sau3AI GATC 7 cut(s) 39, 152, 158, 244, 375, 394, 580
SchI GAGTC 3 cut(s) 223, 424, 596
ScrFI CCNGG 2 cut(s) 65, 137
SduI GDGCHC 2 cut(s) 62, 258
SfaNI GCATC 2 cut(s) 640, 755
Sfr274I CTCGAG 1 cut(s) 251
SfuI TTCGAA 1 cut(s) 674
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 2 cut(s) 65, 458
SmlI CTYRAG 2 cut(s) 251, 275
SmoI CTYRAG 2 cut(s) 251, 275
Sse9I AATT 3 cut(s) 328, 338, 814
SspMI CTAG 1 cut(s) 561
StyD4I CCNGG 2 cut(s) 63, 135
StyI CCWWGG 2 cut(s) 204, 555
TaaI ACNGT 3 cut(s) 283, 405, 638
TaiI ACGT 2 cut(s) 363, 450
TasI AATT 3 cut(s) 328, 338, 814
TauI GCSGC 3 cut(s) 473, 624, 627
TfiI GAWTC 1 cut(s) 599
Tru1I TTAA 2 cut(s) 78, 765
Tru9I TTAA 2 cut(s) 78, 765
TscAI CASTG 1 cut(s) 861
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 3 cut(s) 4, 433, 609
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 3 cut(s) 243, 591, 672
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 1 cut(s) 861
VspI ATTAAT 1 cut(s) 78
XapI RAATTY 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XhoI CTCGAG 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 1 cut(s) 561
ZraI GACGTC 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.