RLG00000014508

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0018134
Physical Location & Seq
Forward (+)
54109243 .. 54109850
608 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014508

Sequence Viewer

Length: 465 bp
ATGCGCAGCTGGAAGATCATGAATGGGTGTAAGGGCACCCAGGCGTTTACCATTAATCCATCTGGAATAACCCCTGCAAATGGTGGAGTAGAAGACAAGCCTTGCCTCAAGACCGTTGATTTTGTCGAAACACTGGCTGATGTGTACCGCAGGATTGAGAATTTTCTGCAATTTGAGAAATCTAAGATGTACGTGGAGCAATGCGCGACCTTTTGGGGTACGTCGGATCCGAAACTATTCTGGTGGAGTCTCCAGTCGGCGAGGCAGCACGCGGTGGATGTCCATACCAAAGTGGTGCTTGCGGCGTGGTTCAGGTACGAGAGGAGGGAGGATGAGCTGGTTGGGTCATCTTCAATGACTTGTTGCAGTAGAAATGTTGAATGTCCCAAGACTAGTTTGGTAGCTGGGTATGATCCGAAGTCTGTCTATGAAGAGGACTTGATATATGAGAAAATTGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

155

Amino Acids

17.62

Weight (kDa)

5.75

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 35
AccII CGCG 2 cut(s) 206, 272
AciI CCGC 3 cut(s) 148, 272, 302
AclWI GGATC 3 cut(s) 221, 234, 407
AcsI RAATTY 1 cut(s) 160
AdeI CACNNNGTG 1 cut(s) 274
AfaI GTAC 4 cut(s) 146, 191, 220, 317
AfiI CCNNNNNNNGG 1 cut(s) 80
AgsI TTSAA 2 cut(s) 354, 380
AhlI ACTAGT 1 cut(s) 392
AjnI CCWGG 1 cut(s) 39
AluBI AGCT 3 cut(s) 9, 337, 404
AluI AGCT 3 cut(s) 9, 337, 404
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 3 cut(s) 221, 234, 407
ApeKI GCWGC 2 cut(s) 6, 265
ApoI RAATTY 1 cut(s) 160
AseI ATTAAT 1 cut(s) 54
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 2 cut(s) 6, 206
BaeGI GKGCMC 1 cut(s) 38
BamHI GGATCC 1 cut(s) 226
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 99
BbvI GCAGC 2 cut(s) 18, 277
BccI CCATC 1 cut(s) 67
BciT130I CCWGG 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 254
BcuI ACTAGT 1 cut(s) 392
BfaI CTAG 1 cut(s) 393
BisI GCNGC 3 cut(s) 7, 266, 303
BlsI GCNGC 3 cut(s) 8, 267, 304
Bme1390I CCNGG 1 cut(s) 41
BmiI GGNNCC 2 cut(s) 37, 228
BmrFI CCNGG 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 99
BpmI CTGGAG 1 cut(s) 236
BpuEI CTTGAG 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 193
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 80
Bse1I ACTGG 2 cut(s) 138, 253
Bse3DI GCAATG 1 cut(s) 206
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 283, 337
BseLI CCNNNNNNNGG 1 cut(s) 80
BseMI GCAATG 1 cut(s) 206
BseNI ACTGG 2 cut(s) 138, 253
BseRI GAGGAG 1 cut(s) 337
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 2 cut(s) 18, 277
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 2 cut(s) 206, 272
BshNI GGYRCC 1 cut(s) 35
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 80
BsmAI GTCTC 1 cut(s) 254
BsmFI GGGAC 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 15, 226, 412
BspACI CCGC 3 cut(s) 148, 272, 302
BspFNI CGCG 2 cut(s) 206, 272
BspHI TCATGA 1 cut(s) 18
BspLI GGNNCC 2 cut(s) 37, 228
BspPI GGATC 3 cut(s) 221, 234, 407
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 206
BsrI ACTGG 2 cut(s) 138, 253
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 3 cut(s) 15, 226, 412
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 1 cut(s) 115
Bst6I CTCTTC 1 cut(s) 426
BstBAI YACGTR 1 cut(s) 193
BstC8I GCNNGC 2 cut(s) 270, 300
BstDEI CTNAG 1 cut(s) 183
BstF5I GGATG 2 cut(s) 283, 337
BstFNI CGCG 2 cut(s) 206, 272
BstHHI GCGC 2 cut(s) 6, 206
BstKTI GATC 3 cut(s) 18, 229, 415
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 3 cut(s) 15, 226, 412
BstNI CCWGG 1 cut(s) 41
BstSCI CCNGG 1 cut(s) 39
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 2 cut(s) 206, 272
BstV1I GCAGC 2 cut(s) 18, 277
BstV2I GAAGAC 1 cut(s) 99
BstX2I RGATCY 1 cut(s) 226
BstYI RGATCY 1 cut(s) 226
BtsCI GGATG 2 cut(s) 283, 337
BtsIMutI CAGTG 1 cut(s) 131
Cac8I GCNNGC 2 cut(s) 270, 300
CciI TCATGA 1 cut(s) 18
CfoI GCGC 2 cut(s) 6, 206
Csp6I GTAC 4 cut(s) 145, 190, 219, 316
CviAII CATG 1 cut(s) 19
CviJI RGCY 5 cut(s) 9, 100, 137, 337, 404
CviKI_1 RGCY 5 cut(s) 9, 100, 137, 337, 404
CviQI GTAC 4 cut(s) 145, 190, 219, 316
DdeI CTNAG 1 cut(s) 183
DpnI GATC 3 cut(s) 17, 228, 414
DpnII GATC 3 cut(s) 15, 226, 412
DraIII CACNNNGTG 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
EcoRII CCWGG 1 cut(s) 39
FaeI CATG 1 cut(s) 22
FaiI YATR 6 cut(s) 20, 285, 411, 429, 445, 447
FalI AAGNNNNNCTT 2 cut(s) 282, 314
FaqI GGGAC 1 cut(s) 369
FatI CATG 1 cut(s) 18
Fnu4HI GCNGC 3 cut(s) 7, 266, 303
FokI GGATG 2 cut(s) 290, 344
Fsp4HI GCNGC 3 cut(s) 7, 266, 303
FspBI CTAG 1 cut(s) 393
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 2 cut(s) 5, 205
GluI GCNGC 3 cut(s) 7, 266, 303
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 236
HhaI GCGC 2 cut(s) 6, 206
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 2 cut(s) 4, 204
HinP1I GCGC 2 cut(s) 4, 204
HinfI GANTC 1 cut(s) 247
Hpy166II GTNNAC 2 cut(s) 48, 145
Hpy188I TCNGA 3 cut(s) 226, 231, 417
Hpy188III TCNNGA 3 cut(s) 19, 63, 109
Hpy8I GTNNAC 2 cut(s) 48, 145
Hpy99I CGWCG 1 cut(s) 226
HpyCH4III ACNGT 1 cut(s) 115
HpyCH4IV ACGT 2 cut(s) 192, 221
HpyCH4V TGCA 4 cut(s) 77, 169, 366, 458
HpyF3I CTNAG 1 cut(s) 183
HpySE526I ACGT 2 cut(s) 192, 221
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 2 cut(s) 4, 204
Kzo9I GATC 3 cut(s) 15, 226, 412
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 2 cut(s) 18, 277
MaeI CTAG 1 cut(s) 393
MaeII ACGT 2 cut(s) 192, 221
MalI GATC 3 cut(s) 17, 228, 414
MboI GATC 3 cut(s) 15, 226, 412
MboII GAAGA 4 cut(s) 25, 104, 342, 443
MflI RGATCY 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 3 cut(s) 160, 170, 453
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 6 cut(s) 116, 255, 315, 318, 322, 427
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 9
MspR9I CCNGG 1 cut(s) 41
MvaI CCWGG 1 cut(s) 41
MvnI CGCG 2 cut(s) 206, 272
NdeII GATC 3 cut(s) 15, 226, 412
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 2 cut(s) 37, 228
NsbI TGCGCA 1 cut(s) 5
PagI TCATGA 1 cut(s) 18
PcsI WCGNNNNNNNCGW 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 236
PkrI GCNGC 3 cut(s) 8, 267, 304
PleI GAGTC 1 cut(s) 255
PpsI GAGTC 1 cut(s) 255
Ppu21I YACGTR 1 cut(s) 193
PshBI ATTAAT 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 37, 228
PsuI RGATCY 1 cut(s) 226
PvuII CAGCTG 1 cut(s) 9
RsaI GTAC 4 cut(s) 146, 191, 220, 317
RsaNI GTAC 4 cut(s) 145, 190, 219, 316
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 3 cut(s) 7, 266, 303
Sau3AI GATC 3 cut(s) 15, 226, 412
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 38
SetI ASST 7 cut(s) 11, 195, 212, 224, 317, 339, 406
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 392
Sse9I AATT 3 cut(s) 160, 170, 453
SsiI CCGC 3 cut(s) 148, 272, 302
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 1 cut(s) 39
TaaI ACNGT 1 cut(s) 115
TaiI ACGT 2 cut(s) 195, 224
TaqI TCGA 1 cut(s) 126
TasI AATT 3 cut(s) 160, 170, 453
TauI GCSGC 1 cut(s) 305
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 1 cut(s) 138
TseI GCWGC 2 cut(s) 6, 265
TspDTI ATGAA 2 cut(s) 35, 444
TspRI CASTG 1 cut(s) 138
VspI ATTAAT 1 cut(s) 54
XapI RAATTY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.