RLG00000031287

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018134
Physical Location & Seq
Reverse (-)
3735304 .. 3736037
734 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031287

Sequence Viewer

Length: 606 bp
ATGAGCTGGAAGATCATGAATGGGTGTAAGGGCACCCAGGCGTTTACCATTAATCCATCTGGAATAACCCCTGCAAATGGTGGAGTAGTAGACAAGGTTGGTCGCAGCCAGGTCAACTCGGTTCGATCTAGATCAAACTGGAGTTTCCAAGCCCCGAACCCGACCGGAAACAATGCCTTGGTTGAGAGTCTTCTTCCCTATGAACTCCCATCGTTAGATCTCCTTGAGACCCAAATCGAGCCTTGCCTCAAGACCGTTGATTTTGTCGAAACACTAGCTAATGTGTACCGCAGGATTGAGAATTTTCTGCAATTCGAGAAATCTAAGATGTACGTAGAGCAATGCGCGACCTTTCGGGGTACGTCGGATCTGAAATTGTTCTGGCGGAGTCTCCAGTCGGTGAGGCAGCACGCGGTGGATGTCCATACCAAAGTGGTGCTTGCGGCGTGGTTCAAGTACGAGAGGAGGGAGGATGAGCTGGTTGGGTCATCTTTAATGACTTGTTGCAGTAGAAATGTTGAATGTCCCAAGGCTAGTTTGGTAGCTGGGTATGATCCGAAGTCTGTTTATAAAGAGGACTTGATATATGAGAAAATTGCAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

202

Amino Acids

22.7

Weight (kDa)

7.56

Isoelectric Point (pI)

41.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 570
AccB1I GGYRCC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 90
AccII CGCG 2 cut(s) 347, 413
AciI CCGC 4 cut(s) 289, 385, 413, 443
AclWI GGATC 2 cut(s) 375, 548
AcsI RAATTY 1 cut(s) 301
AdeI CACNNNGTG 1 cut(s) 415
AfaI GTAC 4 cut(s) 287, 332, 361, 458
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 2 cut(s) 454, 521
AjnI CCWGG 2 cut(s) 36, 108
AluBI AGCT 4 cut(s) 6, 278, 478, 545
AluI AGCT 4 cut(s) 6, 278, 478, 545
Alw26I GTCTC 2 cut(s) 221, 395
AlwI GGATC 2 cut(s) 375, 548
ApeKI GCWGC 2 cut(s) 105, 406
ApoI RAATTY 1 cut(s) 301
AseI ATTAAT 1 cut(s) 51
Asp700I GAANNNNTTC 1 cut(s) 377
AspLEI GCGC 1 cut(s) 347
AsuHPI GGTGA 1 cut(s) 412
BaeGI GKGCMC 1 cut(s) 35
BanI GGYRCC 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 117, 418
BccI CCATC 2 cut(s) 64, 217
BciT130I CCWGG 2 cut(s) 38, 110
BcoDI GTCTC 2 cut(s) 221, 395
BfaI CTAG 3 cut(s) 129, 275, 534
BglII AGATCT 1 cut(s) 217
BisI GCNGC 3 cut(s) 106, 407, 444
BlsI GCNGC 3 cut(s) 107, 408, 445
Bme1390I CCNGG 2 cut(s) 38, 110
BmiI GGNNCC 1 cut(s) 34
BmrFI CCNGG 2 cut(s) 38, 110
BpiI GAAGAC 1 cut(s) 182
BpmI CTGGAG 2 cut(s) 160, 377
BpuEI CTTGAG 2 cut(s) 233, 245
BsaAI YACGTR 1 cut(s) 334
BsaBI GATNNNNATC 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 221
BsaJI CCNNGG 3 cut(s) 36, 177, 528
BsaWI WCCGGW 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 77
Bse1I ACTGG 2 cut(s) 143, 394
Bse3DI GCAATG 1 cut(s) 347
Bse8I GATNNNNATC 1 cut(s) 130
BseBI CCWGG 2 cut(s) 38, 110
BseDI CCNNGG 3 cut(s) 36, 177, 528
BseGI GGATG 2 cut(s) 424, 478
BseJI GATNNNNATC 1 cut(s) 130
BseLI CCNNNNNNNGG 1 cut(s) 77
BseMI GCAATG 1 cut(s) 347
BseNI ACTGG 2 cut(s) 143, 394
BseRI GAGGAG 1 cut(s) 478
BseSI GKGCMC 1 cut(s) 35
BseXI GCAGC 2 cut(s) 117, 418
BseYI CCCAGC 1 cut(s) 545
Bsh1236I CGCG 2 cut(s) 347, 413
Bsh1285I CGRYCG 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 32
BsiEI CGRYCG 1 cut(s) 165
BsiSI CCGG 1 cut(s) 165
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 2 cut(s) 221, 395
BsmFI GGGAC 1 cut(s) 510
Bso31I GGTCTC 1 cut(s) 221
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 6 cut(s) 12, 125, 131, 217, 367, 553
BspACI CCGC 4 cut(s) 289, 385, 413, 443
BspFNI CGCG 2 cut(s) 347, 413
BspHI TCATGA 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 34
BspPI GGATC 2 cut(s) 375, 548
BspT107I GGYRCC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 347
BsrI ACTGG 2 cut(s) 143, 394
BssECI CCNNGG 3 cut(s) 36, 177, 528
BssMI GATC 6 cut(s) 12, 125, 131, 217, 367, 553
BssT1I CCWWGG 2 cut(s) 177, 528
Bst2UI CCWGG 2 cut(s) 38, 110
Bst4CI ACNGT 1 cut(s) 256
BstBAI YACGTR 1 cut(s) 334
BstC8I GCNNGC 2 cut(s) 411, 441
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 2 cut(s) 424, 478
BstFNI CGCG 2 cut(s) 347, 413
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 6 cut(s) 15, 128, 134, 220, 370, 556
BstMAI GTCTC 2 cut(s) 221, 395
BstMBI GATC 6 cut(s) 12, 125, 131, 217, 367, 553
BstMCI CGRYCG 1 cut(s) 165
BstNI CCWGG 2 cut(s) 38, 110
BstSCI CCNGG 2 cut(s) 36, 108
BstSLI GKGCMC 1 cut(s) 35
BstSNI TACGTA 1 cut(s) 334
BstUI CGCG 2 cut(s) 347, 413
BstV1I GCAGC 2 cut(s) 117, 418
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 217, 367
BstYI RGATCY 2 cut(s) 217, 367
BtsCI GGATG 2 cut(s) 424, 478
Cac8I GCNNGC 2 cut(s) 411, 441
CciI TCATGA 1 cut(s) 15
CfoI GCGC 1 cut(s) 347
Csp6I GTAC 4 cut(s) 286, 331, 360, 457
CviAII CATG 1 cut(s) 16
CviJI RGCY 8 cut(s) 6, 108, 152, 241, 278, 478, 533, 545
CviKI_1 RGCY 8 cut(s) 6, 108, 152, 241, 278, 478, 533, 545
CviQI GTAC 4 cut(s) 286, 331, 360, 457
DdeI CTNAG 1 cut(s) 324
DpnI GATC 6 cut(s) 14, 127, 133, 219, 369, 555
DpnII GATC 6 cut(s) 12, 125, 131, 217, 367, 553
DraIII CACNNNGTG 1 cut(s) 415
EciI GGCGGA 1 cut(s) 400
Eco105I TACGTA 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 177, 528
Eco31I GGTCTC 1 cut(s) 221
EcoRII CCWGG 2 cut(s) 36, 108
EcoT14I CCWWGG 2 cut(s) 177, 528
ErhI CCWWGG 2 cut(s) 177, 528
FaeI CATG 1 cut(s) 19
FaiI YATR 7 cut(s) 17, 201, 426, 552, 570, 586, 588
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FaqI GGGAC 1 cut(s) 510
FatI CATG 1 cut(s) 15
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 106, 407, 444
FokI GGATG 2 cut(s) 431, 485
Fsp4HI GCNGC 3 cut(s) 106, 407, 444
FspBI CTAG 3 cut(s) 129, 275, 534
GlaI GCGC 1 cut(s) 346
GluI GCNGC 3 cut(s) 106, 407, 444
GsaI CCCAGC 1 cut(s) 549
GsuI CTGGAG 2 cut(s) 160, 377
HapII CCGG 1 cut(s) 165
HhaI GCGC 1 cut(s) 347
Hin1II CATG 1 cut(s) 19
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 2 cut(s) 187, 388
HpaII CCGG 1 cut(s) 165
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 4 cut(s) 45, 91, 115, 286
Hpy188I TCNGA 3 cut(s) 367, 372, 558
Hpy188III TCNNGA 5 cut(s) 16, 60, 129, 250, 316
Hpy8I GTNNAC 4 cut(s) 45, 91, 115, 286
Hpy99I CGWCG 1 cut(s) 367
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 2 cut(s) 333, 362
HpyCH4V TGCA 4 cut(s) 74, 310, 507, 599
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 2 cut(s) 333, 362
Hsp92II CATG 1 cut(s) 19
HspAI GCGC 1 cut(s) 345
Kzo9I GATC 6 cut(s) 12, 125, 131, 217, 367, 553
Lsp1109I GCAGC 2 cut(s) 117, 418
MaeI CTAG 3 cut(s) 129, 275, 534
MaeII ACGT 2 cut(s) 333, 362
MalI GATC 6 cut(s) 14, 127, 133, 219, 369, 555
MboI GATC 6 cut(s) 12, 125, 131, 217, 367, 553
MboII GAAGA 3 cut(s) 22, 182, 185
MflI RGATCY 2 cut(s) 217, 367
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 4 cut(s) 301, 311, 374, 594
MlyI GAGTC 2 cut(s) 196, 397
MmeI TCCRAC 1 cut(s) 345
MnlI CCTC 6 cut(s) 257, 396, 456, 459, 463, 568
MroXI GAANNNNTTC 1 cut(s) 377
MseI TTAA 2 cut(s) 51, 494
MspI CCGG 1 cut(s) 165
MspR9I CCNGG 2 cut(s) 38, 110
MvaI CCWGG 2 cut(s) 38, 110
MvnI CGCG 2 cut(s) 347, 413
NdeII GATC 6 cut(s) 12, 125, 131, 217, 367, 553
NlaIII CATG 1 cut(s) 19
NlaIV GGNNCC 1 cut(s) 34
PagI TCATGA 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 377
PkrI GCNGC 3 cut(s) 107, 408, 445
PleI GAGTC 2 cut(s) 195, 396
PpsI GAGTC 2 cut(s) 195, 396
Ppu21I YACGTR 1 cut(s) 334
PshBI ATTAAT 1 cut(s) 51
PsiI TTATAA 1 cut(s) 570
Psp6I CCWGG 2 cut(s) 36, 108
PspFI CCCAGC 1 cut(s) 545
PspGI CCWGG 2 cut(s) 36, 108
PspN4I GGNNCC 1 cut(s) 34
PsuI RGATCY 2 cut(s) 217, 367
RsaI GTAC 4 cut(s) 287, 332, 361, 458
RsaNI GTAC 4 cut(s) 286, 331, 360, 457
SaqAI TTAA 2 cut(s) 51, 494
SatI GCNGC 3 cut(s) 106, 407, 444
Sau3AI GATC 6 cut(s) 12, 125, 131, 217, 367, 553
SchI GAGTC 2 cut(s) 196, 397
ScrFI CCNGG 2 cut(s) 38, 110
SduI GDGCHC 1 cut(s) 35
SetI ASST 9 cut(s) 8, 99, 114, 280, 336, 353, 365, 480, 547
SmlI CTYRAG 2 cut(s) 224, 248
SmoI CTYRAG 2 cut(s) 224, 248
SnaBI TACGTA 1 cut(s) 334
Sse9I AATT 4 cut(s) 301, 311, 374, 594
SsiI CCGC 4 cut(s) 289, 385, 413, 443
SspMI CTAG 3 cut(s) 129, 275, 534
StyD4I CCNGG 2 cut(s) 36, 108
StyI CCWWGG 2 cut(s) 177, 528
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 2 cut(s) 336, 365
TaqI TCGA 4 cut(s) 124, 237, 267, 315
TasI AATT 4 cut(s) 301, 311, 374, 594
TauI GCSGC 1 cut(s) 446
Tru1I TTAA 2 cut(s) 51, 494
Tru9I TTAA 2 cut(s) 51, 494
TseI GCWGC 2 cut(s) 105, 406
TspDTI ATGAA 2 cut(s) 32, 216
VspI ATTAAT 1 cut(s) 51
XapI RAATTY 1 cut(s) 301
XbaI TCTAGA 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 535
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 377
XspI CTAG 3 cut(s) 129, 275, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.