Rmu_co8278443.1_g000001

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8278443.1
OG0018134
Physical Location & Seq
Forward (+)
235 .. 843
609 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8278443.1_g000001.1.cds

Sequence Viewer

Length: 609 bp
atgcgtagcttgaagatcatggatgggtgtaagggcacccaggtgtttgccattaacccgtctggaacaacccccacaaatggtggagtaggagacaaggttggtcacagccgggtcaactcggttcgatccagatcgaactggagtttccaagccccgaacccgaccggaaacaatgccttggtcgagagtcttcttccctatgaactcccatcgtcggatctcctcgaaccccaaatcgagccttgcctcaagaccgttgattttgtcgaaacgctggctgatgtgtaccgcaggattgagaattgcccgcaattcgagaaatctaagatgtacgtggagcaatgcgcgatctttcggggtatgtcggatccgaaactgttccggcggagtctccggtcggcgaggcagcacgcggtggatgtccacacgaaagtggtgcttgcggcgtggttgaggtacgagaggagggaggatgagctggttgggtcatcttcgatgacttgttgcggtagaaatgttgaatgtcccaaggctagtttggcagctgggtatgatccggagtctgtttatgattcatgttgctgctctcggagcggtggcgatgtg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

203

Amino Acids

22.43

Weight (kDa)

7.49

Isoelectric Point (pI)

57.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 35
AccBSI CCGCTC 1 cut(s) 597
AccII CGCG 2 cut(s) 350, 416
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 7 cut(s) 292, 311, 388, 416, 446, 510, 597
AclWI GGATC 5 cut(s) 123, 228, 365, 378, 551
AdeI CACNNNGTG 1 cut(s) 418
AfaI GTAC 3 cut(s) 290, 335, 461
AfiI CCNNNNNNNGG 2 cut(s) 80, 217
AgsI TTSAA 2 cut(s) 13, 524
AjnI CCWGG 1 cut(s) 39
AleI CACNNNNGTG 2 cut(s) 41, 434
AluBI AGCT 3 cut(s) 9, 481, 548
AluI AGCT 3 cut(s) 9, 481, 548
Alw26I GTCTC 2 cut(s) 87, 398
AlwI GGATC 5 cut(s) 123, 228, 365, 378, 551
Aor13HI TCCGGA 1 cut(s) 559
ApeKI GCWGC 3 cut(s) 409, 545, 585
Asp700I GAANNNNTTC 1 cut(s) 380
AspLEI GCGC 1 cut(s) 350
AsuC2I CCSGG 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 38
BamHI GGATCC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 35
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 3 cut(s) 421, 557, 572
BccI CCATC 2 cut(s) 17, 220
BcgI CGANNNNNNTGC 2 cut(s) 421, 455
BciT130I CCWGG 1 cut(s) 41
BcnI CCSGG 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 87, 398
BfaI CTAG 1 cut(s) 537
BisI GCNGC 4 cut(s) 410, 447, 546, 586
BlsI GCNGC 4 cut(s) 411, 448, 547, 587
Bme1390I CCNGG 2 cut(s) 41, 113
BmiI GGNNCC 2 cut(s) 37, 372
BmrFI CCNGG 2 cut(s) 41, 113
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 163
BpuEI CTTGAG 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 337
BsaBI GATNNNNATC 1 cut(s) 133
BsaJI CCNNGG 3 cut(s) 39, 180, 531
BsaWI WCCGGW 3 cut(s) 167, 396, 559
BsaXI ACNNNNNCTCC 2 cut(s) 84, 114
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 217
Bse1I ACTGG 1 cut(s) 146
Bse3DI GCAATG 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 133
BseAI TCCGGA 1 cut(s) 559
BseBI CCWGG 1 cut(s) 41
BseDI CCNNGG 3 cut(s) 39, 180, 531
BseGI GGATG 3 cut(s) 28, 427, 481
BseJI GATNNNNATC 1 cut(s) 133
BseLI CCNNNNNNNGG 2 cut(s) 80, 217
BseMI GCAATG 1 cut(s) 350
BseNI ACTGG 1 cut(s) 146
BseRI GAGGAG 2 cut(s) 215, 481
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 3 cut(s) 421, 557, 572
BseYI CCCAGC 1 cut(s) 548
Bsh1236I CGCG 2 cut(s) 350, 416
Bsh1285I CGRYCG 2 cut(s) 168, 401
BshNI GGYRCC 1 cut(s) 35
BsiEI CGRYCG 2 cut(s) 168, 401
BsiSI CCGG 5 cut(s) 112, 168, 385, 397, 560
BslFI GGGAC 1 cut(s) 513
BslI CCNNNNNNNGG 2 cut(s) 80, 217
BsmAI GTCTC 2 cut(s) 87, 398
BsmFI GGGAC 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 38
Bsp13I TCCGGA 1 cut(s) 559
Bsp143I GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
BspACI CCGC 7 cut(s) 292, 311, 388, 416, 446, 510, 597
BspEI TCCGGA 1 cut(s) 559
BspFNI CGCG 2 cut(s) 350, 416
BspLI GGNNCC 2 cut(s) 37, 372
BspPI GGATC 5 cut(s) 123, 228, 365, 378, 551
BspT107I GGYRCC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 597
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 146
BssECI CCNNGG 3 cut(s) 39, 180, 531
BssMI GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
BssT1I CCWWGG 2 cut(s) 180, 531
Bst2UI CCWGG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 259, 381
BstBAI YACGTR 1 cut(s) 337
BstC8I GCNNGC 4 cut(s) 279, 311, 414, 444
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 3 cut(s) 28, 427, 481
BstFNI CGCG 2 cut(s) 350, 416
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 7 cut(s) 18, 131, 137, 223, 354, 373, 559
BstMAI GTCTC 2 cut(s) 87, 398
BstMBI GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
BstMCI CGRYCG 2 cut(s) 168, 401
BstMWI GCNNNNNNNGC 2 cut(s) 542, 594
BstNI CCWGG 1 cut(s) 41
BstSCI CCNGG 2 cut(s) 39, 111
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 2 cut(s) 350, 416
BstV1I GCAGC 3 cut(s) 421, 557, 572
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 2 cut(s) 220, 370
BstYI RGATCY 2 cut(s) 220, 370
BtsCI GGATG 3 cut(s) 28, 427, 481
Cac8I GCNNGC 4 cut(s) 279, 311, 414, 444
CfoI GCGC 1 cut(s) 350
Csp6I GTAC 3 cut(s) 289, 334, 460
CviAII CATG 2 cut(s) 19, 579
CviJI RGCY 8 cut(s) 9, 111, 155, 244, 281, 481, 536, 548
CviKI_1 RGCY 8 cut(s) 9, 111, 155, 244, 281, 481, 536, 548
CviQI GTAC 3 cut(s) 289, 334, 460
DdeI CTNAG 1 cut(s) 327
DpnI GATC 7 cut(s) 17, 130, 136, 222, 353, 372, 558
DpnII GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
DraIII CACNNNGTG 1 cut(s) 418
EciI GGCGGA 1 cut(s) 403
Eco130I CCWWGG 2 cut(s) 180, 531
EcoRII CCWGG 1 cut(s) 39
EcoT14I CCWWGG 2 cut(s) 180, 531
ErhI CCWWGG 2 cut(s) 180, 531
FaeI CATG 2 cut(s) 22, 582
FaiI YATR 6 cut(s) 20, 204, 365, 555, 573, 580
FalI AAGNNNNNCTT 2 cut(s) 426, 458
FaqI GGGAC 1 cut(s) 513
FatI CATG 2 cut(s) 18, 578
FauI CCCGC 1 cut(s) 318
Fnu4HI GCNGC 4 cut(s) 410, 447, 546, 586
FokI GGATG 3 cut(s) 35, 434, 488
Fsp4HI GCNGC 4 cut(s) 410, 447, 546, 586
FspBI CTAG 1 cut(s) 537
GlaI GCGC 1 cut(s) 349
GluI GCNGC 4 cut(s) 410, 447, 546, 586
GsaI CCCAGC 1 cut(s) 552
GsuI CTGGAG 1 cut(s) 163
HapII CCGG 5 cut(s) 112, 168, 385, 397, 560
HhaI GCGC 1 cut(s) 350
Hin1II CATG 2 cut(s) 22, 582
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HinfI GANTC 4 cut(s) 190, 391, 563, 575
HpaII CCGG 5 cut(s) 112, 168, 385, 397, 560
Hpy166II GTNNAC 3 cut(s) 118, 289, 427
Hpy188I TCNGA 4 cut(s) 220, 370, 375, 594
Hpy188III TCNNGA 6 cut(s) 63, 132, 187, 253, 319, 560
Hpy8I GTNNAC 3 cut(s) 118, 289, 427
Hpy99I CGWCG 1 cut(s) 220
HpyCH4III ACNGT 2 cut(s) 259, 381
HpyCH4IV ACGT 1 cut(s) 336
HpyF10VI GCNNNNNNNGC 2 cut(s) 542, 594
HpyF3I CTNAG 1 cut(s) 327
HpySE526I ACGT 1 cut(s) 336
Hsp92II CATG 2 cut(s) 22, 582
HspAI GCGC 1 cut(s) 348
Kpn2I TCCGGA 1 cut(s) 559
Kzo9I GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
LmnI GCTCC 2 cut(s) 340, 594
Lsp1109I GCAGC 3 cut(s) 421, 557, 572
MaeI CTAG 1 cut(s) 537
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 104
MalI GATC 7 cut(s) 17, 130, 136, 222, 353, 372, 558
MbiI CCGCTC 1 cut(s) 597
MboI GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
MboII GAAGA 4 cut(s) 25, 185, 188, 486
MflI RGATCY 2 cut(s) 220, 370
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 2 cut(s) 304, 314
MlyI GAGTC 3 cut(s) 199, 400, 572
MmeI TCCRAC 2 cut(s) 198, 348
MnlI CCTC 7 cut(s) 236, 260, 399, 450, 459, 462, 466
MroI TCCGGA 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 1 cut(s) 54
MslI CAYNNNNRTG 2 cut(s) 41, 434
MspA1I CMGCKG 1 cut(s) 548
MspI CCGG 5 cut(s) 112, 168, 385, 397, 560
MspR9I CCNGG 2 cut(s) 41, 113
MvaI CCWGG 1 cut(s) 41
MvnI CGCG 2 cut(s) 350, 416
MwoI GCNNNNNNNGC 2 cut(s) 542, 594
NciI CCSGG 1 cut(s) 113
NdeII GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
NlaIII CATG 2 cut(s) 22, 582
NlaIV GGNNCC 2 cut(s) 37, 372
NmuCI GTSAC 1 cut(s) 104
OliI CACNNNNGTG 2 cut(s) 41, 434
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 1 cut(s) 575
PkrI GCNGC 4 cut(s) 411, 448, 547, 587
PleI GAGTC 3 cut(s) 198, 399, 571
PpsI GAGTC 3 cut(s) 198, 399, 571
Ppu21I YACGTR 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 548
PspGI CCWGG 1 cut(s) 39
PspN4I GGNNCC 2 cut(s) 37, 372
PsuI RGATCY 2 cut(s) 220, 370
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 3 cut(s) 290, 335, 461
RsaNI GTAC 3 cut(s) 289, 334, 460
RseI CAYNNNNRTG 2 cut(s) 41, 434
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 4 cut(s) 410, 447, 546, 586
Sau3AI GATC 7 cut(s) 15, 128, 134, 220, 351, 370, 556
SchI GAGTC 3 cut(s) 199, 400, 572
ScrFI CCNGG 2 cut(s) 41, 113
SduI GDGCHC 1 cut(s) 38
SetI ASST 7 cut(s) 11, 45, 102, 339, 461, 483, 550
SmiMI CAYNNNNRTG 2 cut(s) 41, 434
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
Sse9I AATT 2 cut(s) 304, 314
SsiI CCGC 7 cut(s) 292, 311, 388, 416, 446, 510, 597
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 2 cut(s) 39, 111
StyI CCWWGG 2 cut(s) 180, 531
TaaI ACNGT 2 cut(s) 259, 381
TaiI ACGT 1 cut(s) 339
TaqI TCGA 8 cut(s) 127, 137, 186, 228, 240, 270, 318, 497
TasI AATT 2 cut(s) 304, 314
TauI GCSGC 1 cut(s) 449
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 3 cut(s) 409, 545, 585
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 2 cut(s) 219, 567
XcmI CCANNNNNNNNNTGG 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.