pycom12g17020

CRAL/TRIO, N-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010322
Physical Location & Seq
Forward (+)
19302728 .. 19304578
1851 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1227 bp
ATGGACCAGAAAACCCCTCTGCAAGCCCTCCCGGAAGCCTCCCCAAAACCCAGCAAGAAAAGCTTCGTCACGTCCCTAATGTCCCCCCGCACCCCCTCCTTCCATGAAGACACCTACTTCGTCTCCAACCTCAAATCCTCCGAAAAGAAATCCCTCCAAGAACTCAAAGACAAGCTCGCCGCCTCGGACTCCTCCTCCGCCACCATGTGGGGCATTCCCCTTCTGGGAAACGACGAAAAGGCCGACGTCGTCCTCCTTAAGTTCCTCCGCGCCAGGGACTTCCGCGTCCCCGACACTTTCACCATGCTAACCAAATGCCTGGCGTGGCGGAAGGAGTTCGGCGCCGACAGCGTCGTGGAGGAGGACTTGGGGTTCAAGGAGCTGGAGGGGGTGGTGGCGTACATGCACGGATTCGACAAACAGGGGCACCCTGTTTGCTACAACGACTATGGAGTTTTTAAAGATAAGGAAATGTATGAGAGGACTTTCGGGGACGATGAGAAGCTGAAGAAGTTTCTGAGGTGGAGAGTGCAGGTTCTTGAGAGGGGGATCAATGCCCTGCACTTTAAGCCAGGTGGGATCAATTCGCTAATTCAAGTCACTGATCTGAAGGACATGCCTAAAAGGGAGCTCAGGGTTGCTTCCAACCAGATCCTCTCTCTGTTTCAGGACAACTATCCTGAAATGGTTGCAAGAAAGATTTTCATCAATGTGCCGTGGTACTTCAGTGTTTTGTATTCGATGTTCAGCCCATTTCTGACTCAGAGAACAAAGAGCAAGTTTGTGATGGCCAAGGAAGGAAATGTTGCAGAAACACTATACAAGTTCATAAGGCCCGAGGATGTTCCGGTTCAGTACGGAGGCCTGAGTCGACCGAGTGATGCGCAAAACGGCCCCCCCAAACCAGCTTCCGAGTTCACTGTCAAAGGAGGAGAGAAAGTGAACATACAGATTGAGGGCATTGAGGCTGATGCAACAATCACATGGGACATAGTAGTGGGAGGGTGGGACTTGGAGTACACTGCAGAGTTCGTGCCTAGTGCAGAAGGCAGCTACACTATCCAAGTGGAGACGCCAAGAAAAGTGATGCCCTCTGAAGAAGCAATCCACAGCTCCTTTACTCCTAGTGAAGCAGGCAAGATGGTTTTCTCAGTTGACAACTCTGCCTCCCGCCGGAAAAAAGTAGCTGCCTATCGCTACATTGTCTGCAAATCTGCTCCCGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

409

Amino Acids

46.08

Weight (kDa)

7.62

Isoelectric Point (pI)

40.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 52 - 105 4e-12 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 129 - 288 1e-33 CRAL/TRIO domain
GOLD_PATL1_C PF25099 309 - 393 4.2e-29 Patellin-1-like, C-terminal GOLD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AatII GACGTC 1 cut(s) 249
Acc16I TGCGCA 1 cut(s) 885
Acc36I ACCTGC 1 cut(s) 523
AccB1I GGYRCC 2 cut(s) 341, 426
AccB7I CCANNNNNTGG 1 cut(s) 207
AccI GTMKAC 1 cut(s) 871
AccII CGCG 2 cut(s) 270, 285
AciI CCGC 7 cut(s) 88, 180, 198, 268, 283, 328, 1171
AclWI GGATC 3 cut(s) 557, 587, 646
AcoI YGGCCR 1 cut(s) 789
AcuI CTGAAG 4 cut(s) 527, 629, 709, 1116
AcyI GRCGYC 3 cut(s) 246, 342, 1073
AdeI CACNNNGTG 1 cut(s) 207
AfaI GTAC 4 cut(s) 401, 722, 857, 1019
AfiI CCNNNNNNNGG 4 cut(s) 207, 224, 274, 1173
AflII CTTAAG 1 cut(s) 257
AgsI TTSAA 2 cut(s) 376, 596
AjiI CACGTC 1 cut(s) 72
AjnI CCWGG 3 cut(s) 272, 318, 571
AloI GAACNNNNNNTCC 2 cut(s) 356, 388
AluBI AGCT 9 cut(s) 63, 175, 382, 505, 631, 908, 1053, 1113, 1187
AluI AGCT 9 cut(s) 63, 175, 382, 505, 631, 908, 1053, 1113, 1187
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 2 cut(s) 127, 1064
AlwI GGATC 3 cut(s) 557, 587, 646
Ama87I CYCGRG 1 cut(s) 836
AoxI GGCC 5 cut(s) 240, 789, 833, 862, 892
ApeKI GCWGC 2 cut(s) 1050, 1187
ArsI GACNNNNNNTTYG 2 cut(s) 101, 133
Asp700I GAANNNNTTC 2 cut(s) 62, 335
AspLEI GCGC 3 cut(s) 272, 344, 886
AspS9I GGNCC 3 cut(s) 4, 834, 893
AsuC2I CCSGG 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 292
AvaI CYCGRG 1 cut(s) 836
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 429
BaeI ACNNNNGTAYC 2 cut(s) 712, 745
BalI TGGCCA 1 cut(s) 791
BanI GGYRCC 2 cut(s) 341, 426
BanII GRGCYC 1 cut(s) 633
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 1062, 1174
BccI CCATC 2 cut(s) 781, 1135
BceAI ACGGC 2 cut(s) 700, 907
BciT130I CCWGG 3 cut(s) 274, 320, 573
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 2 cut(s) 127, 1064
BfaI CTAG 3 cut(s) 1038, 1125, 1225
BfmI CTRYAG 1 cut(s) 1023
BfoI RGCGCY 1 cut(s) 345
BfrI CTTAAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 523
BisI GCNGC 3 cut(s) 180, 1051, 1188
BlsI GCNGC 3 cut(s) 181, 1052, 1189
Bme1390I CCNGG 4 cut(s) 32, 274, 320, 573
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 836
BmgBI CACGTC 1 cut(s) 72
BmgT120I GGNCC 3 cut(s) 4, 834, 893
BmiI GGNNCC 3 cut(s) 343, 428, 895
BmrFI CCNGG 4 cut(s) 32, 274, 320, 573
BmsI GCATC 3 cut(s) 871, 961, 1077
BpiI GAAGAC 1 cut(s) 114
BpmI CTGGAG 1 cut(s) 404
Bpu10I CCTNAGC 1 cut(s) 632
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 1 cut(s) 32
BsaBI GATNNNNATC 1 cut(s) 704
BsaHI GRCGYC 3 cut(s) 246, 342, 1073
BsaJI CCNNGG 5 cut(s) 183, 273, 716, 792, 837
BsaWI WCCGGW 1 cut(s) 847
BsaXI ACNNNNNCTCC 8 cut(s) 107, 137, 179, 209, 924, 954, 1151, 1181
Bsc4I CCNNNNNNNGG 4 cut(s) 207, 224, 274, 1173
Bse8I GATNNNNATC 1 cut(s) 704
BseBI CCWGG 3 cut(s) 274, 320, 573
BseDI CCNNGG 5 cut(s) 183, 273, 716, 792, 837
BseGI GGATG 1 cut(s) 847
BseJI GATNNNNATC 1 cut(s) 704
BseLI CCNNNNNNNGG 4 cut(s) 207, 224, 274, 1173
BseMII CTCAG 5 cut(s) 509, 646, 776, 857, 1164
BseRI GAGGAG 4 cut(s) 181, 184, 374, 945
BseSI GKGCMC 1 cut(s) 429
BseXI GCAGC 2 cut(s) 1062, 1174
BseYI CCCAGC 1 cut(s) 50
BsgI GTGCAG 3 cut(s) 545, 551, 1062
Bsh1236I CGCG 2 cut(s) 270, 285
Bsh1285I CGRYCG 1 cut(s) 875
BshFI GGCC 5 cut(s) 242, 791, 835, 864, 894
BshNI GGYRCC 2 cut(s) 341, 426
BsiEI CGRYCG 1 cut(s) 875
BsiHKAI GWGCWC 1 cut(s) 633
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 3 cut(s) 32, 848, 1174
BslFI GGGAC 7 cut(s) 58, 67, 272, 290, 506, 1001, 1022
BslI CCNNNNNNNGG 4 cut(s) 207, 224, 274, 1173
BsmAI GTCTC 2 cut(s) 127, 1064
BsmBI CGTCTC 2 cut(s) 127, 1064
BsmFI GGGAC 7 cut(s) 58, 67, 272, 290, 506, 1001, 1022
BsmI GAATGC 1 cut(s) 213
BsnI GGCC 5 cut(s) 242, 791, 835, 864, 894
BsoBI CYCGRG 1 cut(s) 836
Bsp1286I GDGCHC 2 cut(s) 429, 633
Bsp143I GATC 4 cut(s) 549, 579, 604, 651
BspACI CCGC 7 cut(s) 88, 180, 198, 268, 283, 328, 1171
BspANI GGCC 5 cut(s) 242, 791, 835, 864, 894
BspCNI CTCAG 5 cut(s) 510, 645, 775, 858, 1163
BspFNI CGCG 2 cut(s) 270, 285
BspLI GGNNCC 3 cut(s) 343, 428, 895
BspMAI CTGCAG 1 cut(s) 1027
BspMI ACCTGC 1 cut(s) 523
BspPI GGATC 3 cut(s) 557, 587, 646
BspT107I GGYRCC 2 cut(s) 341, 426
BspTI CTTAAG 1 cut(s) 257
BssECI CCNNGG 5 cut(s) 183, 273, 716, 792, 837
BssMI GATC 4 cut(s) 549, 579, 604, 651
BssNI GRCGYC 3 cut(s) 246, 342, 1073
BssT1I CCWWGG 1 cut(s) 792
Bst2UI CCWGG 3 cut(s) 274, 320, 573
Bst4CI ACNGT 1 cut(s) 922
BstACI GRCGYC 3 cut(s) 246, 342, 1073
BstAFI CTTAAG 1 cut(s) 257
BstC8I GCNNGC 3 cut(s) 24, 177, 1135
BstDEI CTNAG 5 cut(s) 518, 632, 762, 866, 1150
BstDSI CCRYGG 1 cut(s) 716
BstF5I GGATG 1 cut(s) 847
BstFNI CGCG 2 cut(s) 270, 285
BstH2I RGCGCY 1 cut(s) 345
BstHHI GCGC 3 cut(s) 272, 344, 886
BstKTI GATC 4 cut(s) 552, 582, 607, 654
BstMAI GTCTC 2 cut(s) 127, 1064
BstMBI GATC 4 cut(s) 549, 579, 604, 651
BstMCI CGRYCG 1 cut(s) 875
BstMWI GCNNNNNNNGC 3 cut(s) 60, 348, 568
BstNI CCWGG 3 cut(s) 274, 320, 573
BstNSI RCATGY 2 cut(s) 406, 619
BstSCI CCNGG 4 cut(s) 30, 272, 318, 571
BstSFI CTRYAG 1 cut(s) 1023
BstSLI GKGCMC 1 cut(s) 429
BstUI CGCG 2 cut(s) 270, 285
BstV1I GCAGC 2 cut(s) 1062, 1174
BstV2I GAAGAC 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 651
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 1 cut(s) 651
BsuRI GGCC 5 cut(s) 242, 791, 835, 864, 894
BtgI CCRYGG 1 cut(s) 716
BtrI CACGTC 1 cut(s) 72
BtsCI GGATG 1 cut(s) 847
BtsI GCAGTG 1 cut(s) 1020
BtsIMutI CAGTG 4 cut(s) 600, 733, 918, 1020
BveI ACCTGC 1 cut(s) 523
Cac8I GCNNGC 3 cut(s) 24, 177, 1135
CfoI GCGC 3 cut(s) 272, 344, 886
Cfr13I GGNCC 3 cut(s) 4, 834, 893
CseI GACGC 3 cut(s) 274, 340, 1081
Csp6I GTAC 4 cut(s) 400, 721, 856, 1018
CviAII CATG 6 cut(s) 104, 205, 304, 403, 616, 984
CviQI GTAC 4 cut(s) 400, 721, 856, 1018
DdeI CTNAG 5 cut(s) 518, 632, 762, 866, 1150
DinI GGCGCC 1 cut(s) 343
DpnI GATC 4 cut(s) 551, 581, 606, 653
DpnII GATC 4 cut(s) 549, 579, 604, 651
DraI TTTAAA 1 cut(s) 460
DraIII CACNNNGTG 1 cut(s) 207
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 789
EciI GGCGGA 2 cut(s) 187, 343
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 792
Eco147I AGGCCT 1 cut(s) 864
Eco24I GRGCYC 1 cut(s) 633
Eco47I GGWCC 1 cut(s) 4
Eco53kI GAGCTC 1 cut(s) 631
Eco57I CTGAAG 4 cut(s) 527, 629, 709, 1116
Eco88I CYCGRG 1 cut(s) 836
EcoICRI GAGCTC 1 cut(s) 631
EcoRII CCWGG 3 cut(s) 272, 318, 571
EcoT14I CCWWGG 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 633
EgeI GGCGCC 1 cut(s) 343
EheI GGCGCC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 792
Esp3I CGTCTC 2 cut(s) 127, 1064
FaeI CATG 6 cut(s) 107, 208, 307, 406, 619, 987
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 7 cut(s) 58, 67, 272, 290, 506, 1001, 1022
FatI CATG 6 cut(s) 103, 204, 303, 402, 615, 983
FauI CCCGC 2 cut(s) 95, 1178
FblI GTMKAC 1 cut(s) 871
Fnu4HI GCNGC 3 cut(s) 180, 1051, 1188
FokI GGATG 1 cut(s) 854
FriOI GRGCYC 1 cut(s) 633
Fsp4HI GCNGC 3 cut(s) 180, 1051, 1188
FspBI CTAG 3 cut(s) 1038, 1125, 1225
FspI TGCGCA 1 cut(s) 885
GlaI GCGC 3 cut(s) 271, 343, 885
GluI GCNGC 3 cut(s) 180, 1051, 1188
GsaI CCCAGC 1 cut(s) 54
GsuI CTGGAG 1 cut(s) 404
HaeII RGCGCY 1 cut(s) 345
HaeIII GGCC 5 cut(s) 242, 791, 835, 864, 894
HapII CCGG 3 cut(s) 32, 848, 1174
HgaI GACGC 3 cut(s) 274, 340, 1081
HhaI GCGC 3 cut(s) 272, 344, 886
Hin1I GRCGYC 3 cut(s) 246, 342, 1073
Hin1II CATG 6 cut(s) 107, 208, 307, 406, 619, 987
Hin6I GCGC 3 cut(s) 270, 342, 884
HinP1I GCGC 3 cut(s) 270, 342, 884
HincII GTYRAC 2 cut(s) 872, 1156
HindII GTYRAC 2 cut(s) 872, 1156
HindIII AAGCTT 1 cut(s) 61
HinfI GANTC 4 cut(s) 188, 411, 760, 868
HpaII CCGG 3 cut(s) 32, 848, 1174
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 5 cut(s) 872, 918, 943, 1020, 1156
Hpy188I TCNGA 8 cut(s) 142, 187, 519, 609, 759, 765, 913, 1096
Hpy188III TCNNGA 3 cut(s) 539, 668, 680
Hpy8I GTNNAC 5 cut(s) 872, 918, 943, 1020, 1156
Hpy99I CGWCG 4 cut(s) 236, 248, 251, 356
HpyAV CCTTC 6 cut(s) 109, 230, 325, 604, 791, 1040
HpyCH4III ACNGT 1 cut(s) 922
HpyCH4IV ACGT 2 cut(s) 71, 246
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 348, 568
HpyF3I CTNAG 5 cut(s) 518, 632, 762, 866, 1150
HpySE526I ACGT 2 cut(s) 71, 246
Hsp92I GRCGYC 3 cut(s) 246, 342, 1073
Hsp92II CATG 6 cut(s) 107, 208, 307, 406, 619, 987
HspAI GCGC 3 cut(s) 270, 342, 884
KasI GGCGCC 1 cut(s) 341
Kzo9I GATC 4 cut(s) 549, 579, 604, 651
LmnI GCTCC 4 cut(s) 379, 628, 1118, 1222
Lsp1109I GCAGC 2 cut(s) 1062, 1174
LweI GCATC 3 cut(s) 871, 961, 1077
MaeI CTAG 3 cut(s) 1038, 1125, 1225
MaeII ACGT 2 cut(s) 71, 246
MaeIII GTNAC 2 cut(s) 67, 598
MalI GATC 4 cut(s) 551, 581, 606, 653
MboI GATC 4 cut(s) 549, 579, 604, 651
MboII GAAGA 3 cut(s) 119, 520, 1109
MflI RGATCY 1 cut(s) 651
MhlI GDGCHC 2 cut(s) 429, 633
MlsI TGGCCA 1 cut(s) 791
MluCI AATT 2 cut(s) 583, 591
MluNI TGGCCA 1 cut(s) 791
Mly113I GGCGCC 1 cut(s) 342
MlyI GAGTC 3 cut(s) 182, 754, 877
MmeI TCCRAC 2 cut(s) 150, 669
Mox20I TGGCCA 1 cut(s) 791
MroXI GAANNNNTTC 2 cut(s) 62, 335
MscI TGGCCA 1 cut(s) 791
MseI TTAA 3 cut(s) 258, 459, 567
MslI CAYNNNNRTG 2 cut(s) 710, 995
Msp20I TGGCCA 1 cut(s) 791
MspCI CTTAAG 1 cut(s) 257
MspI CCGG 3 cut(s) 32, 848, 1174
MspR9I CCNGG 4 cut(s) 32, 274, 320, 573
Mva1269I GAATGC 1 cut(s) 213
MvaI CCWGG 3 cut(s) 274, 320, 573
MvnI CGCG 2 cut(s) 270, 285
MwoI GCNNNNNNNGC 3 cut(s) 60, 348, 568
NarI GGCGCC 1 cut(s) 342
NciI CCSGG 1 cut(s) 32
NdeII GATC 4 cut(s) 549, 579, 604, 651
NlaIII CATG 6 cut(s) 107, 208, 307, 406, 619, 987
NlaIV GGNNCC 3 cut(s) 343, 428, 895
NmuCI GTSAC 2 cut(s) 67, 598
NsbI TGCGCA 1 cut(s) 885
NspI RCATGY 2 cut(s) 406, 619
PceI AGGCCT 1 cut(s) 864
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 213
PdmI GAANNNNTTC 2 cut(s) 62, 335
PfeI GAWTC 1 cut(s) 411
PflFI GACNNNGTC 2 cut(s) 248, 350
PflMI CCANNNNNTGG 1 cut(s) 207
PfoI TCCNGGA 1 cut(s) 30
PkrI GCNGC 3 cut(s) 181, 1052, 1189
PleI GAGTC 3 cut(s) 182, 754, 876
PluTI GGCGCC 1 cut(s) 345
PpsI GAGTC 3 cut(s) 182, 754, 876
Psp124BI GAGCTC 1 cut(s) 633
Psp6I CCWGG 3 cut(s) 272, 318, 571
PspFI CCCAGC 1 cut(s) 50
PspGI CCWGG 3 cut(s) 272, 318, 571
PspN4I GGNNCC 3 cut(s) 343, 428, 895
PspPI GGNCC 3 cut(s) 4, 834, 893
PsrI GAACNNNNNNTAC 2 cut(s) 728, 760
PstI CTGCAG 1 cut(s) 1027
PsuI RGATCY 1 cut(s) 651
PsyI GACNNNGTC 2 cut(s) 248, 350
RsaI GTAC 4 cut(s) 401, 722, 857, 1019
RsaNI GTAC 4 cut(s) 400, 721, 856, 1018
RseI CAYNNNNRTG 2 cut(s) 710, 995
SacI GAGCTC 1 cut(s) 633
SalI GTCGAC 1 cut(s) 870
SaqAI TTAA 3 cut(s) 258, 459, 567
SatI GCNGC 3 cut(s) 180, 1051, 1188
Sau3AI GATC 4 cut(s) 549, 579, 604, 651
Sau96I GGNCC 3 cut(s) 4, 834, 893
SchI GAGTC 3 cut(s) 182, 754, 877
ScrFI CCNGG 4 cut(s) 32, 274, 320, 573
SduI GDGCHC 2 cut(s) 429, 633
SfaNI GCATC 3 cut(s) 871, 961, 1077
SfcI CTRYAG 1 cut(s) 1023
SfoI GGCGCC 1 cut(s) 343
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 710, 995
SmlI CTYRAG 2 cut(s) 257, 539
SmoI CTYRAG 2 cut(s) 257, 539
Sse9I AATT 2 cut(s) 583, 591
SseBI AGGCCT 1 cut(s) 864
SsiI CCGC 7 cut(s) 88, 180, 198, 268, 283, 328, 1171
SspDI GGCGCC 1 cut(s) 341
SspMI CTAG 3 cut(s) 1038, 1125, 1225
SstI GAGCTC 1 cut(s) 633
StuI AGGCCT 1 cut(s) 864
StyD4I CCNGG 4 cut(s) 30, 272, 318, 571
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 1 cut(s) 922
TaiI ACGT 2 cut(s) 74, 249
TaqI TCGA 3 cut(s) 414, 740, 871
TaqII GACCGA 1 cut(s) 889
TasI AATT 2 cut(s) 583, 591
TatI WGTACW 1 cut(s) 1017
TauI GCSGC 1 cut(s) 182
TfiI GAWTC 1 cut(s) 411
Tru1I TTAA 3 cut(s) 258, 459, 567
Tru9I TTAA 3 cut(s) 258, 459, 567
TscAI CASTG 4 cut(s) 607, 733, 925, 1027
TseFI GTSAC 2 cut(s) 67, 598
TseI GCWGC 2 cut(s) 1050, 1187
Tsp45I GTSAC 2 cut(s) 67, 598
TspDTI ATGAA 3 cut(s) 120, 694, 817
TspGWI ACGGA 2 cut(s) 423, 873
TspRI CASTG 4 cut(s) 607, 733, 925, 1027
Tth111I GACNNNGTC 2 cut(s) 248, 350
Van91I CCANNNNNTGG 1 cut(s) 207
Vha464I CTTAAG 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 4
XceI RCATGY 2 cut(s) 406, 619
XmiI GTMKAC 1 cut(s) 871
XmnI GAANNNNTTC 2 cut(s) 62, 335
XspI CTAG 3 cut(s) 1038, 1125, 1225
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.