pycom12g18660

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009550
Physical Location & Seq
Forward (+)
20781533 .. 20782033
501 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 291 bp
ATGGCGTTGAGGCAGGTTTTAGGGTTCTCGGATGGCGAGTTGATGAGGTCGGATTGCAAACCTTGTTCCAGATTGATGAGACAGACTGCCGGTATTTTCACTGTTGGCGGATCATTGGGATTCTGGATCCTTTGCAGATTGCATTACGGTCCGAGAATTACAGTTCCAAGGAGTCTTCGGTGGGCAGCTTGTGGAGCGGTATCTGTGAGCTCATCCACTGCTTTGCTGGTTCGGTTGTTTAGTCCAGAGTGCGAGCCCCAAAACATAGCTACTTATGACAAGGGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

97

Amino Acids

10.54

Weight (kDa)

9.49

Isoelectric Point (pI)

62.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7875 PF25285 1 - 95 9.7e-56 Domain of unknown function (DUF7875)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 4
AccBSI CCGCTC 1 cut(s) 197
AciI CCGC 2 cut(s) 108, 197
AclWI GGATC 3 cut(s) 118, 121, 134
AluBI AGCT 3 cut(s) 188, 210, 269
AluI AGCT 3 cut(s) 188, 210, 269
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 3 cut(s) 118, 121, 134
ApeKI GCWGC 1 cut(s) 185
AspS9I GGNCC 1 cut(s) 149
AvaII GGWCC 1 cut(s) 149
BamHI GGATCC 1 cut(s) 126
BanII GRGCYC 2 cut(s) 212, 258
BbsI GAAGAC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 197
BccI CCATC 1 cut(s) 26
BcoDI GTCTC 1 cut(s) 73
BfuAI ACCTGC 1 cut(s) 4
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 1 cut(s) 128
BpiI GAAGAC 1 cut(s) 167
BsaJI CCNNGG 1 cut(s) 167
Bse118I RCCGGY 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 2 cut(s) 37, 212
BseXI GCAGC 1 cut(s) 197
BsiHKAI GWGCWC 1 cut(s) 212
BsiSI CCGG 1 cut(s) 90
BsmAI GTCTC 1 cut(s) 73
Bsp1286I GDGCHC 2 cut(s) 212, 258
Bsp143I GATC 2 cut(s) 110, 126
BspACI CCGC 2 cut(s) 108, 197
BspLI GGNNCC 1 cut(s) 128
BspMI ACCTGC 1 cut(s) 4
BspPI GGATC 3 cut(s) 118, 121, 134
BsrBI CCGCTC 1 cut(s) 197
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 2 cut(s) 110, 126
BssT1I CCWWGG 1 cut(s) 167
Bst4CI ACNGT 3 cut(s) 103, 149, 163
BstC8I GCNNGC 1 cut(s) 254
BstF5I GGATG 2 cut(s) 37, 212
BstKTI GATC 2 cut(s) 113, 129
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 2 cut(s) 110, 126
BstMWI GCNNNNNNNGC 1 cut(s) 194
BstV1I GCAGC 1 cut(s) 197
BstV2I GAAGAC 1 cut(s) 167
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BtsCI GGATG 2 cut(s) 37, 212
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 2 cut(s) 99, 216
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 1 cut(s) 254
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 149
CpoI CGGWCCG 1 cut(s) 149
CspI CGGWCCG 1 cut(s) 149
CviJI RGCY 4 cut(s) 188, 210, 256, 269
CviKI_1 RGCY 4 cut(s) 188, 210, 256, 269
DpnI GATC 2 cut(s) 112, 128
DpnII GATC 2 cut(s) 110, 126
EciI GGCGGA 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 210
Eco130I CCWWGG 1 cut(s) 167
Eco24I GRGCYC 2 cut(s) 212, 258
Eco47I GGWCC 1 cut(s) 149
Eco53kI GAGCTC 1 cut(s) 210
EcoICRI GAGCTC 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 167
EcoT38I GRGCYC 2 cut(s) 212, 258
ErhI CCWWGG 1 cut(s) 167
FaiI YATR 2 cut(s) 266, 276
Fnu4HI GCNGC 1 cut(s) 186
FokI GGATG 2 cut(s) 44, 199
FriOI GRGCYC 2 cut(s) 212, 258
Fsp4HI GCNGC 1 cut(s) 186
GluI GCNGC 1 cut(s) 186
HapII CCGG 1 cut(s) 90
HinfI GANTC 2 cut(s) 120, 172
HpaII CCGG 1 cut(s) 90
Hpy188I TCNGA 3 cut(s) 31, 52, 153
Hpy188III TCNNGA 3 cut(s) 69, 124, 245
HpyCH4III ACNGT 3 cut(s) 103, 149, 163
HpyCH4V TGCA 3 cut(s) 57, 135, 142
HpyF10VI GCNNNNNNNGC 1 cut(s) 194
Kzo9I GATC 2 cut(s) 110, 126
LmnI GCTCC 1 cut(s) 194
LpnPI CCDG 5 cut(s) 82, 103, 109, 212, 258
Lsp1109I GCAGC 1 cut(s) 197
MalI GATC 2 cut(s) 112, 128
MbiI CCGCTC 1 cut(s) 197
MboI GATC 2 cut(s) 110, 126
MboII GAAGA 1 cut(s) 167
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 2 cut(s) 212, 258
MluCI AATT 1 cut(s) 156
MlyI GAGTC 1 cut(s) 181
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 2 cut(s) 3, 39
MspI CCGG 1 cut(s) 90
MwoI GCNNNNNNNGC 1 cut(s) 194
NdeII GATC 2 cut(s) 110, 126
NlaIV GGNNCC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 120
PkrI GCNGC 1 cut(s) 187
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 212
PspN4I GGNNCC 1 cut(s) 128
PspPI GGNCC 1 cut(s) 149
PsuI RGATCY 1 cut(s) 126
Rsr2I CGGWCCG 1 cut(s) 149
RsrII CGGWCCG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 212
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 110, 126
Sau96I GGNCC 1 cut(s) 149
SchI GAGTC 1 cut(s) 181
SduI GDGCHC 2 cut(s) 212, 258
SetI ASST 6 cut(s) 18, 50, 64, 190, 212, 271
SinI GGWCC 1 cut(s) 149
Sse9I AATT 1 cut(s) 156
SsiI CCGC 2 cut(s) 108, 197
SstI GAGCTC 1 cut(s) 212
StyI CCWWGG 1 cut(s) 167
TaaI ACNGT 3 cut(s) 103, 149, 163
TasI AATT 1 cut(s) 156
TfiI GAWTC 1 cut(s) 120
TscAI CASTG 2 cut(s) 106, 223
TseI GCWGC 1 cut(s) 185
TspRI CASTG 2 cut(s) 106, 223
VpaK11BI GGWCC 1 cut(s) 149
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.