pycom12g21570

Nucleolar protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0011411
Physical Location & Seq
Reverse (-)
22716120 .. 22717644
1525 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGAGGGTTCGAGAATGGTATGGTTGGCATTTTCCAGAGCTTGCTAAGATTGTACAGGACAACATCCATTATGCCAAGGTAGTGAAGCTGATGGGCTACCGTTCTAATGCTGCAAAGCTTGATTTCTCCGAGATATTGCCAGAGGAGGTTGAAACTGAATTGAAGGATGCGGCAATGATATCCATGGGAACAGAAGTTAGTGAGCTTGATTTGATGAACATCAAAGAACTCTGTGACCAAGTCCTTTCTCTCTCTGAGTACAGAGCTCAGCTATATGACTATTTGAAAAATAGGATGAACACCATTGCTCCAAATTTGACTGCTCTTGTTGGAGAGCTCGTCGGTGCTCGCCTCATTTCCCATGGTGGTAGCTTGTTGAACCTTGCAAAGCAGCCTGGGAGCACAGTTCAGATTCTTGGAGCTGAAAAGGCCCTTTTCAGAGCACTGAAGACTAAGCACGCAACACCAAAATATGGACTTATTTACCATGCATCCTTGATTGGTAAAGCAGCGCCAAAGCTGAAGGGTAAAATTTCTCGGTCACTTGCTAGTAAAGCTGCATTGGCAATTCGAGTTGATGCTCTTGGAGACAGTCAAGATAACTCAATGGGAGTGGAAAATTATCAAAAGCTTGAAGCACGGTTAAGGAATCTTGAAGGCAGAGAATTGGGTCACTCTGCTGGGTCGGCTAAAGGCAAACCAAAGATTGAAGTCTATGACAAGGATCGCAAGAAGGGAGCCGCAGGATTGATAACTGCTGCCAAGGCATATAATCCTGCAGCAGATGCGGTTCTTGGGAAAATGAGACCTAATACTGAGAAACCAGCTGCCGAGGTTCCCGTTACTGAAGATAAGAAAGAGAAGAAGAAAAAGAAGAAGAAGGCTGATGATGTGGACATGCCTGACGGAAATGCCAATGTGGAACCAGAAGTTGAGGAGCCTGCAAAGAAGGAAAAGAAGAAGAAGCATTCGGCTGAGGATGCTGAAGTAGTTGCTGTGGTTGAAAATAAAGATGCGGGAGAGAAGAAGAAGAGGAAAAGAAAGCATGCTGAGGAAGAAGAAACGGAGGTGCCAAGCGAGAAGAAAGAGAAAAAGAAGAAGAAGAAGAGCAAGGATTGA

Protein Analysis

373

Amino Acids

41.19

Weight (kDa)

9.38

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop PF01798 1 - 216 1.3e-81 snoRNA binding domain, fibrillarin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05060 AT5G27120
fragaria_vesca FvH4_6g04090 FvH4_6g04090
malus_domestica MD04G1216600.v1.1 MD12G1233200.v1.1
prunus_persica Prupe.6G336300_v2.0.a1
pyrus_communis pycom04g19260 pycom12g21570
rosa_chinensis RchiOBHm_Chr3g0452641
rosa_laevigata RLG00000025576
rosa_multiflora Rmu_sc0022184.1_g000001
rosa_roxburghii Rroxscaffold_6G00429680
rosa_rugosa Rorug02G0642100 Rorug02G0642100
rosa_samantha Rh3AG044500 Rh3BG046800 Rh3CG045300 Rh3DG045700
rosa_wichuraiana Rw3G003470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1069
AccB7I CCANNNNNTGG 1 cut(s) 473
AciI CCGC 4 cut(s) 170, 741, 788, 1016
AclWI GGATC 1 cut(s) 732
AcsI RAATTY 2 cut(s) 313, 531
AcuI CTGAAG 4 cut(s) 467, 542, 867, 1005
AfaI GTAC 2 cut(s) 54, 260
AfiI CCNNNNNNNGG 1 cut(s) 473
AgsI TTSAA 8 cut(s) 152, 163, 286, 379, 635, 656, 710, 1004
AjnI CCWGG 1 cut(s) 394
Alw21I GWGCWC 5 cut(s) 268, 339, 349, 404, 445
Alw26I GTCTC 2 cut(s) 582, 799
AlwI GGATC 1 cut(s) 732
AoxI GGCC 1 cut(s) 429
ApeKI GCWGC 7 cut(s) 110, 391, 509, 557, 758, 779, 827
ApoI RAATTY 2 cut(s) 313, 531
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 1 cut(s) 430
BanI GGYRCC 1 cut(s) 1069
BanII GRGCYC 2 cut(s) 268, 339
BbsI GAAGAC 1 cut(s) 455
Bbv12I GWGCWC 5 cut(s) 268, 339, 349, 404, 445
BbvCI CCTCAGC 2 cut(s) 975, 1050
BbvI GCAGC 7 cut(s) 97, 403, 521, 544, 745, 791, 814
BccI CCATC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 582, 799
BfaI CTAG 1 cut(s) 549
BfmI CTRYAG 1 cut(s) 777
BfoI RGCGCY 1 cut(s) 515
BisI GCNGC 9 cut(s) 111, 171, 392, 510, 558, 741, 759, 780, 828
BlpI GCTNAGC 1 cut(s) 267
BlsI GCNGC 9 cut(s) 112, 172, 393, 511, 559, 742, 760, 781, 829
Bme1390I CCNGG 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 430
BmiI GGNNCC 5 cut(s) 739, 837, 924, 939, 1071
BmrFI CCNGG 1 cut(s) 396
BmsI GCATC 6 cut(s) 157, 500, 568, 775, 970, 1003
BpiI GAAGAC 1 cut(s) 455
Bpu10I CCTNAGC 2 cut(s) 975, 1050
Bpu1102I GCTNAGC 1 cut(s) 267
BsaBI GATNNNNATC 1 cut(s) 218
BsaI GGTCTC 1 cut(s) 799
BsaJI CCNNGG 6 cut(s) 75, 183, 361, 395, 762, 831
BsaXI ACNNNNNCTCC 4 cut(s) 292, 322, 324, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 473
Bse3DI GCAATG 2 cut(s) 180, 303
Bse8I GATNNNNATC 1 cut(s) 218
BseBI CCWGG 1 cut(s) 396
BseDI CCNNGG 6 cut(s) 75, 183, 361, 395, 762, 831
BseGI GGATG 5 cut(s) 63, 172, 300, 491, 985
BseJI GATNNNNATC 1 cut(s) 218
BseLI CCNNNNNNNGG 1 cut(s) 473
BseMI GCAATG 2 cut(s) 180, 303
BseMII CTCAG 5 cut(s) 246, 281, 807, 966, 1041
BseRI GAGGAG 2 cut(s) 158, 950
BseXI GCAGC 7 cut(s) 97, 403, 521, 544, 745, 791, 814
BseYI CCCAGC 1 cut(s) 680
BshFI GGCC 1 cut(s) 431
BshNI GGYRCC 1 cut(s) 1069
BsiHKAI GWGCWC 5 cut(s) 268, 339, 349, 404, 445
BslI CCNNNNNNNGG 1 cut(s) 473
BsmAI GTCTC 2 cut(s) 582, 799
BsmI GAATGC 1 cut(s) 967
BsnI GGCC 1 cut(s) 431
Bso31I GGTCTC 1 cut(s) 799
Bsp1286I GDGCHC 5 cut(s) 268, 339, 349, 404, 445
Bsp1407I TGTACA 1 cut(s) 52
Bsp143I GATC 1 cut(s) 724
Bsp1720I GCTNAGC 1 cut(s) 267
Bsp19I CCATGG 2 cut(s) 183, 361
BspACI CCGC 4 cut(s) 170, 741, 788, 1016
BspANI GGCC 1 cut(s) 431
BspCNI CTCAG 5 cut(s) 247, 280, 808, 967, 1042
BspLI GGNNCC 5 cut(s) 739, 837, 924, 939, 1071
BspMAI CTGCAG 1 cut(s) 781
BspPI GGATC 1 cut(s) 732
BspQI GCTCTTC 1 cut(s) 1100
BspT107I GGYRCC 1 cut(s) 1069
BspTNI GGTCTC 1 cut(s) 799
BsrDI GCAATG 2 cut(s) 180, 303
BsrGI TGTACA 1 cut(s) 52
BssECI CCNNGG 6 cut(s) 75, 183, 361, 395, 762, 831
BssMI GATC 1 cut(s) 724
BssT1I CCWWGG 4 cut(s) 75, 183, 361, 762
Bst2UI CCWGG 1 cut(s) 396
Bst4CI ACNGT 4 cut(s) 101, 406, 593, 642
Bst6I CTCTTC 2 cut(s) 1025, 1100
BstAPI GCANNNNNTGC 1 cut(s) 785
BstAUI TGTACA 1 cut(s) 52
BstC8I GCNNGC 5 cut(s) 42, 349, 459, 942, 1047
BstDEI CTNAG 7 cut(s) 45, 255, 267, 453, 816, 975, 1050
BstDSI CCRYGG 2 cut(s) 183, 361
BstF5I GGATG 5 cut(s) 63, 172, 300, 491, 985
BstH2I RGCGCY 1 cut(s) 515
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 1 cut(s) 727
BstMAI GTCTC 2 cut(s) 582, 799
BstMBI GATC 1 cut(s) 724
BstMWI GCNNNNNNNGC 7 cut(s) 428, 554, 563, 686, 764, 785, 980
BstNI CCWGG 1 cut(s) 396
BstNSI RCATGY 2 cut(s) 901, 1049
BstSCI CCNGG 1 cut(s) 394
BstSFI CTRYAG 1 cut(s) 777
BstV1I GCAGC 7 cut(s) 97, 403, 521, 544, 745, 791, 814
BstV2I GAAGAC 1 cut(s) 455
BsuRI GGCC 1 cut(s) 431
BtgI CCRYGG 2 cut(s) 183, 361
BtsCI GGATG 5 cut(s) 63, 172, 300, 491, 985
BtsIMutI CAGTG 1 cut(s) 443
Cac8I GCNNGC 5 cut(s) 42, 349, 459, 942, 1047
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 1 cut(s) 430
Csp6I GTAC 2 cut(s) 53, 259
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 5 cut(s) 184, 362, 488, 898, 1046
CviQI GTAC 2 cut(s) 53, 259
DdeI CTNAG 7 cut(s) 45, 255, 267, 453, 816, 975, 1050
DpnI GATC 1 cut(s) 726
DpnII GATC 1 cut(s) 724
Eam1104I CTCTTC 2 cut(s) 1025, 1100
EarI CTCTTC 2 cut(s) 1025, 1100
Ecl136II GAGCTC 2 cut(s) 266, 337
Eco130I CCWWGG 4 cut(s) 75, 183, 361, 762
Eco24I GRGCYC 2 cut(s) 268, 339
Eco31I GGTCTC 1 cut(s) 799
Eco32I GATATC 1 cut(s) 180
Eco53kI GAGCTC 2 cut(s) 266, 337
Eco57I CTGAAG 4 cut(s) 467, 542, 867, 1005
EcoICRI GAGCTC 2 cut(s) 266, 337
EcoO109I RGGNCCY 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 394
EcoRV GATATC 1 cut(s) 180
EcoT14I CCWWGG 4 cut(s) 75, 183, 361, 762
EcoT22I ATGCAT 1 cut(s) 493
EcoT38I GRGCYC 2 cut(s) 268, 339
ErhI CCWWGG 4 cut(s) 75, 183, 361, 762
FaeI CATG 5 cut(s) 187, 365, 491, 901, 1049
FatI CATG 5 cut(s) 183, 361, 487, 897, 1045
FauI CCCGC 1 cut(s) 1009
Fnu4HI GCNGC 9 cut(s) 111, 171, 392, 510, 558, 741, 759, 780, 828
FokI GGATG 5 cut(s) 50, 179, 307, 478, 992
FriOI GRGCYC 2 cut(s) 268, 339
Fsp4HI GCNGC 9 cut(s) 111, 171, 392, 510, 558, 741, 759, 780, 828
FspBI CTAG 1 cut(s) 549
GlaI GCGC 1 cut(s) 513
GluI GCNGC 9 cut(s) 111, 171, 392, 510, 558, 741, 759, 780, 828
GsaI CCCAGC 1 cut(s) 684
HaeII RGCGCY 1 cut(s) 515
HaeIII GGCC 1 cut(s) 431
HhaI GCGC 1 cut(s) 514
Hin1II CATG 5 cut(s) 187, 365, 491, 901, 1049
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HindIII AAGCTT 2 cut(s) 116, 629
HinfI GANTC 2 cut(s) 412, 649
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 4 cut(s) 130, 256, 411, 440
Hpy188III TCNNGA 4 cut(s) 11, 35, 596, 653
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 6 cut(s) 157, 517, 650, 727, 874, 943
HpyCH4III ACNGT 4 cut(s) 101, 406, 593, 642
HpyCH4V TGCA 6 cut(s) 113, 386, 491, 560, 779, 944
HpyF10VI GCNNNNNNNGC 7 cut(s) 428, 554, 563, 686, 764, 785, 980
HpyF3I CTNAG 7 cut(s) 45, 255, 267, 453, 816, 975, 1050
Hsp92II CATG 5 cut(s) 187, 365, 491, 901, 1049
HspAI GCGC 1 cut(s) 512
Kzo9I GATC 1 cut(s) 724
LguI GCTCTTC 1 cut(s) 1100
LmnI GCTCC 5 cut(s) 313, 399, 419, 737, 937
Lsp1109I GCAGC 7 cut(s) 97, 403, 521, 544, 745, 791, 814
LweI GCATC 6 cut(s) 157, 500, 568, 775, 970, 1003
MaeI CTAG 1 cut(s) 549
MaeIII GTNAC 4 cut(s) 233, 540, 671, 841
MalI GATC 1 cut(s) 726
MboI GATC 1 cut(s) 724
MhlI GDGCHC 5 cut(s) 268, 339, 349, 404, 445
MluCI AATT 6 cut(s) 158, 313, 531, 567, 619, 665
MmeI TCCRAC 1 cut(s) 310
MnlI CCTC 9 cut(s) 136, 139, 362, 826, 928, 970, 1026, 1045, 1060
Mph1103I ATGCAT 1 cut(s) 493
MseI TTAA 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 827
MspR9I CCNGG 1 cut(s) 396
Mva1269I GAATGC 1 cut(s) 967
MvaI CCWGG 1 cut(s) 396
MwoI GCNNNNNNNGC 7 cut(s) 428, 554, 563, 686, 764, 785, 980
NcoI CCATGG 2 cut(s) 183, 361
NdeII GATC 1 cut(s) 724
NlaIII CATG 5 cut(s) 187, 365, 491, 901, 1049
NlaIV GGNNCC 5 cut(s) 739, 837, 924, 939, 1071
NmeAIII GCCGAG 1 cut(s) 856
NmuCI GTSAC 3 cut(s) 233, 540, 671
NsiI ATGCAT 1 cut(s) 493
NspI RCATGY 2 cut(s) 901, 1049
PaeI GCATGC 1 cut(s) 1049
PciSI GCTCTTC 1 cut(s) 1100
PctI GAATGC 1 cut(s) 967
PfeI GAWTC 2 cut(s) 412, 649
PflFI GACNNNGTC 1 cut(s) 239
PflMI CCANNNNNTGG 1 cut(s) 473
PkrI GCNGC 9 cut(s) 112, 172, 393, 511, 559, 742, 760, 781, 829
Psp124BI GAGCTC 2 cut(s) 268, 339
Psp6I CCWGG 1 cut(s) 394
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 1 cut(s) 394
PspN4I GGNNCC 5 cut(s) 739, 837, 924, 939, 1071
PspPI GGNCC 1 cut(s) 430
PstI CTGCAG 1 cut(s) 781
PsyI GACNNNGTC 1 cut(s) 239
PvuII CAGCTG 1 cut(s) 827
RsaI GTAC 2 cut(s) 54, 260
RsaNI GTAC 2 cut(s) 53, 259
SacI GAGCTC 2 cut(s) 268, 339
SapI GCTCTTC 1 cut(s) 1100
SaqAI TTAA 1 cut(s) 644
SatI GCNGC 9 cut(s) 111, 171, 392, 510, 558, 741, 759, 780, 828
Sau3AI GATC 1 cut(s) 724
Sau96I GGNCC 1 cut(s) 430
ScrFI CCNGG 1 cut(s) 396
SduI GDGCHC 5 cut(s) 268, 339, 349, 404, 445
SfaNI GCATC 6 cut(s) 157, 500, 568, 775, 970, 1003
SfcI CTRYAG 1 cut(s) 777
SphI GCATGC 1 cut(s) 1049
Sse9I AATT 6 cut(s) 158, 313, 531, 567, 619, 665
SsiI CCGC 4 cut(s) 170, 741, 788, 1016
SspMI CTAG 1 cut(s) 549
SstI GAGCTC 2 cut(s) 268, 339
StyD4I CCNGG 1 cut(s) 394
StyI CCWWGG 4 cut(s) 75, 183, 361, 762
TaaI ACNGT 4 cut(s) 101, 406, 593, 642
TaqI TCGA 2 cut(s) 10, 571
TaqII GACCGA 1 cut(s) 528
TasI AATT 6 cut(s) 158, 313, 531, 567, 619, 665
TatI WGTACW 2 cut(s) 52, 258
TauI GCSGC 2 cut(s) 173, 743
TfiI GAWTC 2 cut(s) 412, 649
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TscAI CASTG 1 cut(s) 450
TseFI GTSAC 3 cut(s) 233, 540, 671
TseI GCWGC 7 cut(s) 110, 391, 509, 557, 758, 779, 827
Tsp45I GTSAC 3 cut(s) 233, 540, 671
TspDTI ATGAA 2 cut(s) 230, 311
TspGWI ACGGA 2 cut(s) 921, 1079
TspRI CASTG 1 cut(s) 450
Tth111I GACNNNGTC 1 cut(s) 239
Van91I CCANNNNNTGG 1 cut(s) 473
XapI RAATTY 2 cut(s) 313, 531
XceI RCATGY 2 cut(s) 901, 1049
XspI CTAG 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.