pycom12g24280

Type II inositol 1,4,5-trisphosphate 5-phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010730
Physical Location & Seq
Forward (+)
24634241 .. 24643026
8786 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3294 bp
ATGGATGAAAACGAGGACCTGTTTTCGTCCCCGAGCTTCTCCGCTCCAAAGCTATTCCCGGACTCTTCCTCTTCCTCATCGGAAGATGAGGACAACGTCGTCTCCCTCCACTCCACCTCCAAACGCCTCGATTACATGCTCCAATTCCTTGACCGCAAGCTGTCCGTCATCAACGACGACGACTACAACAAGAACAACAACAGCAGCAGTAACTACAACAATCATAATAATAGGAGCAACGCCTCCGAGGGGAACTGCTTGTCTTCGCTTCCGGAGTTCGTGGCTAGTGGGGGAGGGACTGGGATCTTCAAAGTCCCGATTCGGGCGGCAGTCCATCCGAACCGGCCGCCGAGGCTGGAAGTGAGGCCCCACCCGCTGAGGGAGACTCAGATAGGGTGCTTCTTGCGGACAATGGTGAGCACCGCGTCCCAATTGTGGGTTGGGACGGAGTGCGCCGTTAGGGTTTGGAACTTGAGCGATCTGTACTCGGCGGCAGGACAAGGCGAATCAGGGGATGAGGAGGCGGTGCCGTATAGGGAGTCGGTATGCACATCGGCTGTGATATGCTTGGTTGGGGATGAGGGGAATAAGGTGGTGTGGAGCGGGCATAGGGATGGAAGGATCAGGTGTTGGAAGATGGATTCGGCTCCTACTCCGACGAATCCGTTTAAGGAGGGCTTATCTTGGCAGGCTCACAGAGGCCCCGTTCTTTCAATTGTCATCTCCTGCTATGGGGATCTATGGTCAGGCTCAGAGGGTGGTTCCATCAAGATATGGCCACGGGAAGCTCTTGAAAAGGCTCTCTCCCTAACAGCCGAAGAAAGGCACATGTCTTCTTTGTTGGTCGAGAGATCTTACATTGAACCTTGGACCCAAGTTGCCGTCAATGGTTTCAATAACATATTAACTTCAGATGTTCGCTACTTATTATCTGATCGTTCTGGAGCCAAGCTCTGGACTGCTGGTTATCTCTCATTCGCATTGTGGGATGCTCGTACAAGGGAGTTATTGAAGCTGTTCAGTACAGATGGTCAGATTGAGAATCGAGTGGACATTTCGTCAGCACAGGACTTCTCCGCTGAACCCATTGCTGGATCAAAGAAAGACAAAACACAAAGTTCTTTTGGTTTCTTTCAGCGGTCACGTAATGCCATAATGGGAGCAGCAGATGCTGTTCGTCGAGTTGCAGTGAAGGGTGCCTTTGGAGATGATAATCGAAGAACTGAAGCACTGGTGATAGCAATGGATGGAATGATTTGGACTGGGTGTACAAATGGCCTACTTGTACAATGGGATAGAAATGGCAATCGTATACAAGAATACCACTATCATTCTTCTGCTGTCCAATGCTTTTGTACATTTGGGCTACGTATATGGGTTGGTTATGCAAGTGGTACTGTCCACGTATTGGACCTTGAGGGTAATCTGCTTGGAGGATGGGTGGCTCACAGCAGTCCTGTCATAAAAATGGCTGCAGGAGCGGGTTTTATATTTACCTTGGCTAATCAAGGTGGTATATGTGGATGGAATATCATGTCCCCTGGACCTCTTGACAATATATTGTGCTCAGAGTTAGCCGGCAAAGAATTTACATATACAAGGATAGAAAATTTGAAAATATTGACTGGTACATGGAATGTTGGACAGGGAAGAGCATCTCAGGACTCACTTATATCCTGGCTGGGCTCTGTTGCTGCTACTGTTGGAATTGTTGTTGTCGGGTTGCAAGAAATTGAAATGGGTGCTGGTTTTCTTGCCATGTCTGCAGCCAAAGAAACTGTAGGGCTTGAGGGCAGCTCTGTTGGGCAGTGGTGGCTGGATATGATTGGAAAAACATTGGATGAAGGTTCGACATTTGAGCGCGTTGGGTCCAGGCAATTGGCAGGATTGCTCATTGCTGTGTGGGTGAGAAACAATCTTAGGACTCATGTTGGGGATGTTGATGCTGCAGCAGTTCCATGTGGTTTTGGGCGTGCCATTGGTAACAAGGGAGCTGTTGGTTTGAGGATTAGAATATATGGCCGGGTAATGTGCTTTGTTAATTGTCACTTTGCTGCACACTTAGAAGCCGTTAATCGCCGCAATGCAGACTTCGATCATGTATATCGAACAATGACCTTCTGCCGACCTAACTTTCTCAATTGTGCAGCTGCTAGTGCTTCATCTGCAGTTCAAATGCTTCGTGGCACACATGCTATTGGTAACAATTCTGCAGAAGGGATGCCTGAGTTATCTGAGGCAGACATGGTCATATTTCTTGGCGATTTTAACTATCGACTTGATGGTATATCTTACGATGAAGCTAGAGATTTTGTTTCTCAAAGATGTTTTGATTGGCTTAGAGAAAGGGATCAGCTCCGAGTGGAAATGGAAGCTGGAAATGTCTTCCAAGGAATGCGTGAGGCAGATATTAAGTTTCCTCCCACGTATAAGTTTGAAAGGCATCAAGCTGGTTTAGCAGGATATGATTCTGGTGAGAAGAAGCGCACTCCAGCTTGGTGCGATAGAATTTTGTACCGTGACAGCCGCTCAGCGTCAGTATCAGAGTGCAGCTTAGAATGCCCTGTAGTGTCTTCAATCTCACAGTACGAGGCCTGCATGGATGTGACAGACAGTGATCACAAGCCTGTCCGTTGCATTTTTACTGTTGATATTGCTCGAGTTGATGAGTCTCTAAGGAGACAAGAGTTTGGAGAAGTCCTTAAATCAAATGAGAAAATCAAATGTATTATTGAAGAACAATGCAAGATTCCAGAAACTATTGTCAGTACAAACAACATAATCCTTCAAAACCAGGACACCTCCATTTTGCGTATTACAAATAAATGTGGCGATAAAGATGCTTTTTTTGATATTATTTGCGAAGGACAGTCAATCATTAAGGAGGATGGACCCACATCAGACTATTGTTCCTTTGGCTTTCCACGATGGCTTGAGGTTACTCCTTCAGCTGGCATTATCAGACCAGATCATATAGCTGAGGTGACAGTTCATCACGAGGAGCACCAAACCCCAGAAGAGTTTCTTGATGTTGTTCCACAAACTTGGTGGTGTGAAGACACCGGAGACAAGGAAGTCATATTGGTGGTTAAGGTGCGTGGCAGTTACACTACTGACACAAGACATCACCGTGTATGTGTGCGCCAAAGCTGTTCAGCCAAGACAAACCAAAACGAACCCACTGGAGACAGCACCAGACAAGCACAAGGGACGGTTCTTCGCCGGTCTGATTTTCAACACCTGAGCAGTTCCTATGATGTGGTTGACCATCTCTGGAGTTCGCGTAGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 map00562 map01100 map04070
Pfam Domains
Protein Families
Exo_endo_phos

Protein Analysis

1098

Amino Acids

121.55

Weight (kDa)

5.55

Isoelectric Point (pI)

45.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_IP5PC_F PF23754 126 - 509 2e-182 IP5P C-F beta-propeller
Exo_endo_phos2 PF22669 543 - 878 3.9e-66 Endonuclease/Exonuclease/phosphatase family 2
Exo_endo_phos PF03372 543 - 874 2.5e-07 Endonuclease/Exonuclease/phosphatase family
Ig-like_IP5PC_F PF23755 917 - 1049 9.2e-63 IP5P C-F Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010730)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65580 AT1G65580
fragaria_vesca FvH4_6g00030 FvH4_6g00030
malus_domestica MD12G1260800.v1.1
prunus_persica Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1 Prupe.6G365800_v2.0.a1
pyrus_communis pycom12g24280
rosa_chinensis RchiOBHm_Chr3g0447351
rosa_laevigata RLG00000026007
rosa_multiflora Rmu_ssc0000473.1_g000002
rosa_roxburghii Rroxscaffold_6G00425530
rosa_rugosa Rorug02G0600200 Rorug02G0600300
rosa_samantha Rh3AG000500 Rh3BG000300 Rh3CG000300 Rh3DG000500
rosa_wichuraiana Rw3G000030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 98, 3074
AccB1I GGYRCC 2 cut(s) 526, 1196
AccB7I CCANNNNNTGG 2 cut(s) 954, 1408
AccBSI CCGCTC 4 cut(s) 44, 603, 1481, 2529
AccI GTMKAC 1 cut(s) 1312
AccII CGCG 3 cut(s) 425, 1863, 3283
AccIII TCCGGA 1 cut(s) 271
AclWI GGATC 5 cut(s) 311, 629, 744, 1102, 2358
AcoI YGGCCR 3 cut(s) 344, 776, 2020
AcsI RAATTY 3 cut(s) 1586, 1609, 2508
AcuI CTGAAG 3 cut(s) 894, 1245, 2931
AflIII ACRYGT 1 cut(s) 828
AhdI GACNNNNNGTC 1 cut(s) 1057
AjnI CCWGG 4 cut(s) 1540, 1676, 1871, 2793
Alw21I GWGCWC 3 cut(s) 422, 1568, 3006
Alw26I GTCTC 6 cut(s) 106, 377, 2674, 2676, 3060, 3180
AlwI GGATC 5 cut(s) 311, 629, 744, 1102, 2358
AlwNI CAGNNNCTG 1 cut(s) 1172
Ama87I CYCGRG 2 cut(s) 31, 2658
Aor13HI TCCGGA 1 cut(s) 271
AoxI GGCC 7 cut(s) 344, 365, 700, 776, 1276, 2020, 2592
ApoI RAATTY 3 cut(s) 1586, 1609, 2508
Asp700I GAANNNNTTC 2 cut(s) 1016, 3021
AspLEI GCGC 4 cut(s) 455, 1863, 2487, 3144
AspS9I GGNCC 8 cut(s) 16, 366, 701, 870, 1411, 1544, 1869, 2891
AsuC2I CCSGG 2 cut(s) 59, 2024
AsuHPI GGTGA 6 cut(s) 427, 1246, 1918, 2486, 2995, 3119
AvaI CYCGRG 2 cut(s) 31, 2658
AvaII GGWCC 6 cut(s) 16, 870, 1411, 1544, 1869, 2891
BalI TGGCCA 1 cut(s) 778
BanI GGYRCC 2 cut(s) 526, 1196
BanII GRGCYC 1 cut(s) 1688
BauI CACGAG 1 cut(s) 2996
BbsI GAAGAC 5 cut(s) 255, 825, 2377, 2565, 3063
Bbv12I GWGCWC 3 cut(s) 422, 1568, 3006
BbvCI CCTCAGC 2 cut(s) 377, 2979
BceAI ACGGC 4 cut(s) 440, 514, 866, 2054
BcgI CGANNNNNNTGC 2 cut(s) 2822, 2856
BciT130I CCWGG 4 cut(s) 1542, 1678, 1873, 2795
BclI TGATCA 1 cut(s) 2617
BcnI CCSGG 2 cut(s) 59, 2024
BcoDI GTCTC 6 cut(s) 106, 377, 2674, 2676, 3060, 3180
BfaI CTAG 3 cut(s) 285, 2154, 2304
BfmI CTRYAG 7 cut(s) 1473, 1764, 1779, 1947, 2166, 2211, 2565
BglI GCCNNNNNGGC 1 cut(s) 352
BglII AGATCT 1 cut(s) 851
BlpI GCTNAGC 1 cut(s) 2530
Bme1390I CCNGG 6 cut(s) 59, 1542, 1678, 1873, 2024, 2795
Bme18I GGWCC 6 cut(s) 16, 870, 1411, 1544, 1869, 2891
BmeRI GACNNNNNGTC 1 cut(s) 1057
BmeT110I CYCGRG 2 cut(s) 31, 2658
BmgT120I GGNCC 8 cut(s) 16, 366, 701, 870, 1411, 1544, 1869, 2891
BmrFI CCNGG 6 cut(s) 59, 1542, 1678, 1873, 2024, 2795
BmrI ACTGGG 2 cut(s) 309, 1272
BmsI GCATC 7 cut(s) 979, 1159, 1664, 1933, 2211, 2452, 2830
BmuI ACTGGG 2 cut(s) 309, 1272
BpiI GAAGAC 5 cut(s) 255, 825, 2377, 2565, 3063
BplI GAGNNNNNCTC 6 cut(s) 370, 402, 936, 968, 1781, 1813
BpmI CTGGAG 3 cut(s) 963, 2475, 3204
Bpu10I CCTNAGC 3 cut(s) 377, 2979, 3242
Bpu1102I GCTNAGC 1 cut(s) 2530
BpuEI CTTGAG 4 cut(s) 493, 1436, 1808, 2954
BpuMI CCSGG 2 cut(s) 59, 2024
BsaAI YACGTR 4 cut(s) 1145, 1370, 1405, 2427
BsaBI GATNNNNATC 1 cut(s) 1212
BsaJI CCNNGG 7 cut(s) 246, 350, 779, 866, 1497, 1540, 2389
BsaWI WCCGGW 2 cut(s) 271, 3062
BsaXI ACNNNNNCTCC 7 cut(s) 86, 101, 116, 131, 1425, 1455, 3268
Bse118I RCCGGY 3 cut(s) 342, 1577, 3222
Bse1I ACTGG 5 cut(s) 304, 1236, 1267, 1630, 3187
Bse3DI GCAATG 4 cut(s) 1086, 1248, 1893, 2089
Bse8I GATNNNNATC 1 cut(s) 1212
BseAI TCCGGA 1 cut(s) 271
BseBI CCWGG 4 cut(s) 1542, 1678, 1873, 2795
BseDI CCNNGG 7 cut(s) 246, 350, 779, 866, 1497, 1540, 2389
BseJI GATNNNNATC 1 cut(s) 1212
BseMI GCAATG 4 cut(s) 1086, 1248, 1893, 2089
BseNI ACTGG 5 cut(s) 304, 1236, 1267, 1630, 3187
BseRI GAGGAG 2 cut(s) 533, 3014
BseX3I CGGCCG 1 cut(s) 344
BseYI CCCAGC 1 cut(s) 1681
BsgI GTGCAG 3 cut(s) 2040, 2166, 2569
Bsh1236I CGCG 3 cut(s) 425, 1863, 3283
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 7 cut(s) 346, 367, 702, 778, 1278, 2022, 2594
BshNI GGYRCC 2 cut(s) 526, 1196
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 3 cut(s) 422, 1568, 3006
BsiHKCI CYCGRG 2 cut(s) 31, 2658
BsiSI CCGG 7 cut(s) 59, 272, 343, 1578, 2023, 3063, 3223
BslFI GGGAC 7 cut(s) 13, 299, 310, 412, 457, 1522, 3223
BsmAI GTCTC 6 cut(s) 106, 377, 2674, 2676, 3060, 3180
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 7 cut(s) 13, 299, 310, 412, 457, 1522, 3223
BsmI GAATGC 2 cut(s) 2400, 2564
BsnI GGCC 7 cut(s) 346, 367, 702, 778, 1278, 2022, 2594
BsoBI CYCGRG 2 cut(s) 31, 2658
Bsp1286I GDGCHC 4 cut(s) 422, 1568, 1688, 3006
Bsp13I TCCGGA 1 cut(s) 271
Bsp1407I TGTACA 3 cut(s) 1268, 1285, 1355
Bsp1720I GCTNAGC 1 cut(s) 2530
BspANI GGCC 7 cut(s) 346, 367, 702, 778, 1278, 2022, 2594
BspEI TCCGGA 1 cut(s) 271
BspFNI CGCG 3 cut(s) 425, 1863, 3283
BspMAI CTGCAG 5 cut(s) 1477, 1768, 1951, 2170, 2215
BspPI GGATC 5 cut(s) 311, 629, 744, 1102, 2358
BspQI GCTCTTC 1 cut(s) 1645
BspT107I GGYRCC 2 cut(s) 526, 1196
BsrBI CCGCTC 4 cut(s) 44, 603, 1481, 2529
BsrDI GCAATG 4 cut(s) 1086, 1248, 1893, 2089
BsrFI RCCGGY 3 cut(s) 342, 1577, 3222
BsrGI TGTACA 3 cut(s) 1268, 1285, 1355
BsrI ACTGG 5 cut(s) 304, 1236, 1267, 1630, 3187
BssAI RCCGGY 3 cut(s) 342, 1577, 3222
BssECI CCNNGG 7 cut(s) 246, 350, 779, 866, 1497, 1540, 2389
BssNAI GTATAC 1 cut(s) 1313
BssSI CACGAG 1 cut(s) 2996
BssT1I CCWWGG 3 cut(s) 866, 1497, 2389
Bst1107I GTATAC 1 cut(s) 1313
Bst2BI CACGAG 1 cut(s) 2996
Bst2UI CCWGG 4 cut(s) 1542, 1678, 1873, 2795
Bst6I CTCTTC 4 cut(s) 70, 76, 1645, 3012
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstAUI TGTACA 3 cut(s) 1268, 1285, 1355
BstBAI YACGTR 4 cut(s) 1145, 1370, 1405, 2427
BstC8I GCNNGC 7 cut(s) 158, 605, 690, 1579, 1974, 2596, 2953
BstDSI CCRYGG 1 cut(s) 779
BstFNI CGCG 3 cut(s) 425, 1863, 3283
BstHHI GCGC 4 cut(s) 455, 1863, 2487, 3144
BstMAI GTCTC 6 cut(s) 106, 377, 2674, 2676, 3060, 3180
BstMCI CGRYCG 1 cut(s) 347
BstNI CCWGG 4 cut(s) 1542, 1678, 1873, 2795
BstNSI RCATGY 3 cut(s) 139, 832, 2195
BstSCI CCNGG 6 cut(s) 57, 1540, 1676, 1871, 2022, 2793
BstSFI CTRYAG 7 cut(s) 1473, 1764, 1779, 1947, 2166, 2211, 2565
BstSNI TACGTA 1 cut(s) 1370
BstUI CGCG 3 cut(s) 425, 1863, 3283
BstV2I GAAGAC 5 cut(s) 255, 825, 2377, 2565, 3063
BstX2I RGATCY 3 cut(s) 303, 736, 851
BstXI CCANNNNNNTGG 2 cut(s) 1879, 3045
BstYI RGATCY 3 cut(s) 303, 736, 851
BstZ17I GTATAC 1 cut(s) 1313
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 7 cut(s) 346, 367, 702, 778, 1278, 2022, 2594
BtgI CCRYGG 1 cut(s) 779
BtsI GCAGTG 2 cut(s) 1194, 1814
BtsIMutI CAGTG 5 cut(s) 1194, 1229, 1814, 2620, 3180
Cac8I GCNNGC 7 cut(s) 158, 605, 690, 1579, 1974, 2596, 2953
CaiI CAGNNNCTG 1 cut(s) 1172
CfoI GCGC 4 cut(s) 455, 1863, 2487, 3144
Cfr10I RCCGGY 3 cut(s) 342, 1577, 3222
Cfr13I GGNCC 8 cut(s) 16, 366, 701, 870, 1411, 1544, 1869, 2891
CseI GACGC 2 cut(s) 414, 2523
CspCI CAANNNNNGTGG 2 cut(s) 3029, 3064
DrdI GACNNNNNNGTC 2 cut(s) 98, 3074
DriI GACNNNNNGTC 1 cut(s) 1057
DseDI GACNNNNNNGTC 2 cut(s) 98, 3074
EaeI YGGCCR 3 cut(s) 344, 776, 2020
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 4 cut(s) 70, 76, 1645, 3012
Eam1105I GACNNNNNGTC 1 cut(s) 1057
EarI CTCTTC 4 cut(s) 70, 76, 1645, 3012
EclXI CGGCCG 1 cut(s) 344
Eco105I TACGTA 1 cut(s) 1370
Eco130I CCWWGG 3 cut(s) 866, 1497, 2389
Eco147I AGGCCT 1 cut(s) 2594
Eco24I GRGCYC 1 cut(s) 1688
Eco47I GGWCC 6 cut(s) 16, 870, 1411, 1544, 1869, 2891
Eco52I CGGCCG 1 cut(s) 344
Eco57I CTGAAG 3 cut(s) 894, 1245, 2931
Eco88I CYCGRG 2 cut(s) 31, 2658
EcoO109I RGGNCCY 3 cut(s) 16, 366, 701
EcoRII CCWGG 4 cut(s) 1540, 1676, 1871, 2793
EcoT14I CCWWGG 3 cut(s) 866, 1497, 2389
EcoT38I GRGCYC 1 cut(s) 1688
ErhI CCWWGG 3 cut(s) 866, 1497, 2389
Esp3I CGTCTC 1 cut(s) 106
FalI AAGNNNNNCTT 6 cut(s) 662, 694, 1184, 1216, 3009, 3041
FaqI GGGAC 7 cut(s) 13, 299, 310, 412, 457, 1522, 3223
FauI CCCGC 3 cut(s) 381, 596, 1474
FbaI TGATCA 1 cut(s) 2617
FblI GTMKAC 1 cut(s) 1312
FriOI GRGCYC 1 cut(s) 1688
FspBI CTAG 3 cut(s) 285, 2154, 2304
GlaI GCGC 4 cut(s) 454, 1862, 2486, 3143
GsaI CCCAGC 1 cut(s) 1685
GsuI CTGGAG 3 cut(s) 963, 2475, 3204
HaeIII GGCC 7 cut(s) 346, 367, 702, 778, 1278, 2022, 2594
HapII CCGG 7 cut(s) 59, 272, 343, 1578, 2023, 3063, 3223
HgaI GACGC 2 cut(s) 414, 2523
HhaI GCGC 4 cut(s) 455, 1863, 2487, 3144
Hin6I GCGC 4 cut(s) 453, 1861, 2485, 3142
HinP1I GCGC 4 cut(s) 453, 1861, 2485, 3142
HincII GTYRAC 1 cut(s) 3265
HindII GTYRAC 1 cut(s) 3265
HpaII CCGG 7 cut(s) 59, 272, 343, 1578, 2023, 3063, 3223
HphI GGTGA 6 cut(s) 427, 1246, 1918, 2486, 2995, 3119
Hpy166II GTNNAC 5 cut(s) 1051, 1269, 1313, 1402, 3265
Hpy8I GTNNAC 5 cut(s) 1051, 1269, 1313, 1402, 3265
Hpy99I CGWCG 5 cut(s) 101, 179, 182, 661, 1182
HpyAV CCTTC 8 cut(s) 612, 1186, 1838, 2128, 2210, 2795, 2858, 2955
HpyCH4IV ACGT 5 cut(s) 96, 1144, 1369, 1404, 2426
HpySE526I ACGT 5 cut(s) 96, 1144, 1369, 1404, 2426
HspAI GCGC 4 cut(s) 453, 1861, 2485, 3142
Kpn2I TCCGGA 1 cut(s) 271
KroI GCCGGC 1 cut(s) 1577
KroNI GCCGGC 1 cut(s) 1579
Ksp22I TGATCA 1 cut(s) 2617
LguI GCTCTTC 1 cut(s) 1645
LweI GCATC 7 cut(s) 979, 1159, 1664, 1933, 2211, 2452, 2830
MaeI CTAG 3 cut(s) 285, 2154, 2304
MaeII ACGT 5 cut(s) 96, 1144, 1369, 1404, 2426
MbiI CCGCTC 4 cut(s) 44, 603, 1481, 2529
MfeI CAATTG 4 cut(s) 431, 714, 1877, 2140
MflI RGATCY 3 cut(s) 303, 736, 851
MhlI GDGCHC 4 cut(s) 422, 1568, 1688, 3006
MlsI TGGCCA 1 cut(s) 778
MluNI TGGCCA 1 cut(s) 778
MlyI GAGTC 6 cut(s) 56, 379, 548, 1658, 1918, 2678
MmeI TCCRAC 4 cut(s) 611, 680, 1621, 1684
Mox20I TGGCCA 1 cut(s) 778
MroI TCCGGA 1 cut(s) 271
MroNI GCCGGC 1 cut(s) 1577
MroXI GAANNNNTTC 2 cut(s) 1016, 3021
MscI TGGCCA 1 cut(s) 778
MslI CAYNNNNRTG 6 cut(s) 612, 1466, 1898, 2603, 3083, 3129
Msp20I TGGCCA 1 cut(s) 778
MspA1I CMGCKG 5 cut(s) 376, 1079, 1138, 2150, 2951
MspI CCGG 7 cut(s) 59, 272, 343, 1578, 2023, 3063, 3223
MspR9I CCNGG 6 cut(s) 59, 1542, 1678, 1873, 2024, 2795
MunI CAATTG 4 cut(s) 431, 714, 1877, 2140
Mva1269I GAATGC 2 cut(s) 2400, 2564
MvaI CCWGG 4 cut(s) 1542, 1678, 1873, 2795
MvnI CGCG 3 cut(s) 425, 1863, 3283
NaeI GCCGGC 1 cut(s) 1579
NciI CCSGG 2 cut(s) 59, 2024
NgoMIV GCCGGC 1 cut(s) 1577
NmeAIII GCCGAG 2 cut(s) 375, 467
NmuCI GTSAC 5 cut(s) 1140, 2045, 2519, 2605, 2983
NspI RCATGY 3 cut(s) 139, 832, 2195
PaeR7I CTCGAG 1 cut(s) 2658
PceI AGGCCT 1 cut(s) 2594
PciI ACATGT 1 cut(s) 828
PciSI GCTCTTC 1 cut(s) 1645
PctI GAATGC 2 cut(s) 2400, 2564
PdiI GCCGGC 1 cut(s) 1579
PdmI GAANNNNTTC 2 cut(s) 1016, 3021
PfeI GAWTC 7 cut(s) 319, 506, 641, 661, 1042, 2468, 2749
PflFI GACNNNGTC 2 cut(s) 95, 2246
PflMI CCANNNNNTGG 2 cut(s) 954, 1408
PfoI TCCNGGA 1 cut(s) 57
PleI GAGTC 6 cut(s) 56, 379, 547, 1658, 1918, 2677
PpsI GAGTC 6 cut(s) 56, 379, 547, 1658, 1918, 2677
Ppu21I YACGTR 4 cut(s) 1145, 1370, 1405, 2427
PpuMI RGGWCCY 1 cut(s) 16
PscI ACATGT 1 cut(s) 828
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 4 cut(s) 1540, 1676, 1871, 2793
PspFI CCCAGC 1 cut(s) 1681
PspGI CCWGG 4 cut(s) 1540, 1676, 1871, 2793
PspPI GGNCC 8 cut(s) 16, 366, 701, 870, 1411, 1544, 1869, 2891
PspPPI RGGWCCY 1 cut(s) 16
PspXI VCTCGAGB 1 cut(s) 2658
PstI CTGCAG 5 cut(s) 1477, 1768, 1951, 2170, 2215
PstNI CAGNNNCTG 1 cut(s) 1172
PsuI RGATCY 3 cut(s) 303, 736, 851
PsyI GACNNNGTC 2 cut(s) 95, 2246
PvuII CAGCTG 2 cut(s) 2150, 2951
RseI CAYNNNNRTG 6 cut(s) 612, 1466, 1898, 2603, 3083, 3129
SapI GCTCTTC 1 cut(s) 1645
Sau96I GGNCC 8 cut(s) 16, 366, 701, 870, 1411, 1544, 1869, 2891
SchI GAGTC 6 cut(s) 56, 379, 548, 1658, 1918, 2678
ScrFI CCNGG 6 cut(s) 59, 1542, 1678, 1873, 2024, 2795
SduI GDGCHC 4 cut(s) 422, 1568, 1688, 3006
SfaNI GCATC 7 cut(s) 979, 1159, 1664, 1933, 2211, 2452, 2830
SfcI CTRYAG 7 cut(s) 1473, 1764, 1779, 1947, 2166, 2211, 2565
Sfr274I CTCGAG 1 cut(s) 2658
SinI GGWCC 6 cut(s) 16, 870, 1411, 1544, 1869, 2891
SlaI CTCGAG 1 cut(s) 2658
SmiMI CAYNNNNRTG 6 cut(s) 612, 1466, 1898, 2603, 3083, 3129
SmlI CTYRAG 5 cut(s) 472, 1415, 1787, 2658, 2933
SmoI CTYRAG 5 cut(s) 472, 1415, 1787, 2658, 2933
SnaBI TACGTA 1 cut(s) 1370
SseBI AGGCCT 1 cut(s) 2594
SspI AATATT 1 cut(s) 1620
SspMI CTAG 3 cut(s) 285, 2154, 2304
StuI AGGCCT 1 cut(s) 2594
StyD4I CCNGG 6 cut(s) 57, 1540, 1676, 1871, 2022, 2793
StyI CCWWGG 3 cut(s) 866, 1497, 2389
TaiI ACGT 5 cut(s) 99, 1147, 1372, 1407, 2429
TatI WGTACW 6 cut(s) 483, 1022, 1268, 1285, 1355, 2768
TauI GCSGC 5 cut(s) 329, 349, 494, 2082, 2529
TfiI GAWTC 7 cut(s) 319, 506, 641, 661, 1042, 2468, 2749
TscAI CASTG 5 cut(s) 1194, 1236, 1814, 2620, 3187
TseFI GTSAC 5 cut(s) 1140, 2045, 2519, 2605, 2983
Tsp45I GTSAC 5 cut(s) 1140, 2045, 2519, 2605, 2983
TspDTI ATGAA 5 cut(s) 21, 1857, 2151, 2313, 2981
TspGWI ACGGA 4 cut(s) 154, 461, 654, 2621
TspRI CASTG 5 cut(s) 1194, 1236, 1814, 2620, 3187
Tth111I GACNNNGTC 2 cut(s) 95, 2246
Van91I CCANNNNNTGG 2 cut(s) 954, 1408
VpaK11BI GGWCC 6 cut(s) 16, 870, 1411, 1544, 1869, 2891
XapI RAATTY 3 cut(s) 1586, 1609, 2508
XceI RCATGY 3 cut(s) 139, 832, 2195
XcmI CCANNNNNNNNNTGG 2 cut(s) 437, 3045
XhoI CTCGAG 1 cut(s) 2658
XmiI GTMKAC 1 cut(s) 1312
XmnI GAANNNNTTC 2 cut(s) 1016, 3021
XspI CTAG 3 cut(s) 285, 2154, 2304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.