pycom13g00600

Uncharacterized ACR, COG1678

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009991
Physical Location & Seq
Forward (+)
381920 .. 386237
4318 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3306 bp
ATGACGACGAAGAATAAGAAAGTGGTGTTCTTAGCTTGCTGGGTATTCCTCGCGGCAGCAATCCCAGCCTTTGAAACTAAATCAGACGGCGTAGGCGAATGGCAACTCCTGACAAAGCAAAATTTCTCCTCCCAGATCCGCCTCCACCCTCACATCCTCCTCATCGTCACTCTCCCATGGTCGGGAGAATCTCGATCGCTCATGAGAGATGTGGCTCGATTGGTTACCGATAATCAAGAAGATTTCAGCTCATTGAGATTGAGGTTTATGTACAGAAACGCAGAGAAGGTGTTGGCGGACGCCATTGGAGCTGCCACGGAAGCAGAGGGAACAACAGTTTTGTACTATCACAATTCTGTGTCTTACAAGTATGGCGGAAGACTTAGGGCGCAAAATGTATTGGCCTCGCTCCGTCCGTATGTGGCGGTTGGACCAGAAGAGCTTCCGTTCAAGTCCCTAAAAACTGCGGAGAAATTGAAAGAGTTCGTTGATTCAACTGACAAAGCTTTGATTCTGTTTGAGTTTTGCGAATGGACTTCAAAATTGCTTGCCAGGAGGAAGAATAATGGAACCGATGGAAACGGTTTTGCTCTGCAAGGGAATTTTCTTGGATTCAACATCAGCGGAGAGGCGAGTAGATCACCAGCACACTTGGGGAAGAATATCCAGAAGGGTATTGAAAGTGCAAAGATGTGCGGTGTTGATTATGGGCTTGGTGGTGTTCCTTGGCTTGGGGACTTCAGCTCGGTAAATGACAGTGCTTCTTTTGTGGAGTCTGAGCAAATGAGCTCATTCTGTAATCGCGAAGAATATCAGCTGTTTGACTCTTTCCTCTCGAAGTTCACAACCGTTGCACGGGAGTTCTTTCTACCTCCTGAAAGGTATAAATTTGGTCTGGTCTCAGAAAGATCGATGCTCTCAACTTTTGGGATTGAAGATTCCAGTACATGGTTGACAGTCCTGTACTTATCTGGATGCCCCGGTTGTTCAAAGATTATTAAGAAAGAGGAGGACCTCAAAAATGCTTTACAGATGGATAATTTGGTTGTTATGGAGTTGGAAGGTCTTGGAAACACTTTGAAGCCTTCTTTACCTGCAAACCAACCATCAGCACTCCTATTTGTGGATAGATCATCTGACTTGTCAGAGACTAGAATAAAATGTAAGAAAGCTCTTGATTCTTTTCGAGAGCTGGCACTGCACTGTCATATTTCACGCCAAAGTGGTGGGCAATATGGAGACAAGTCTGAGAAACCCTCGGTCCAAGATTATCAAGCTTTAAGGAGTACATCTGGATATCCAAAACTAAAGTTGTCTCAGACAATCAAATTAAAAGACAAAACGTCTACTTTCATGATTGTAAATGAAGGAAAACAAGTCACTTTGGATAATATAGCTTTGGATCTACAGGGTGGTTCCTTGAAGGAGATCTTGGATATCTTACTTAAGCAAAAGAACAAAGCTAAATTAAGCTCGCTTGCAAAAGAGTTAGGTTTCCAACTTATATCAGATGACATGGACATCAAGTTAGTGAATACATTGCCAGTGCAGAAAGAACTTCAGTCTGATCAACTTACAGAAGAACTGTCTAAGGAAGGCCTTGTGACAAGTAGTGTTAACTCAGACAAGGATCAATTGCCACACAGGACTAGTGTATCTGCTGAAGAGCATCTGGAAACTTCCGAAGTCACAGATTCTGAAATCTTTTCTCAGACTGATGAAGAGAAAACAGCATATGTTGGTACAAGTAAGCAGTTCTTATCTGCAGATAGTGAACAACATCTTGCAGATCATAAACTTGATAGTACTGAAGATATAAAGGTCGATGAAGAAAGTTCTTCACAGGAAGACAAATCAGGGGAGCAACAGCTTCGCTTTCAAGGTTTTGAGGGCTCTTTTTTCTTTTCTGATGGTAATTATCGATTACTTAATAGTCTCACTGGTGGATCAAAAATTCCTGCCTTGGTGATAGTTGATCCCATTGCCCAGCAACATTTTGTCTTCTCAGAAGAGGCTGATCTTAGCTATTCTTCACTGGCTAATTTTCTTTCTGGGTTTGTTAATGGAAGTCTTCTTCCATATCAACAGTCTGAATCTGTTCTTCAAAGCTCTAGGGAGGCCACTCAACCACCATTTGTTAATCTGGATTTTCGTGAAGTGGATTCTGTCCCTCGAGTTACAAGTCGTACCTTCACCGAGCAGGTGATTGGGTTTAATCAATCTGACACTGACGCTTGGAATAAGGATGTCTTGGTCCTTTTTAGCAATAGATGGTGTGGATTTTGCCAGAGAATGGAATTGGTTGTTCGTGAAGTATATCGGGCTATGAAGGACTATATTAAGATGCTGAAAAGCGGATCAAAGAATGAGAAAACAAGGTTTCACGATGGTGACTTGAAGGATGAAATGCTGAAGCTTCCATTATTATACTTGCTTGACTGCACCTTGAATGATTGCAGTGTGATATTGAAATCAATGAATCAGAGGGAAGTTTATCCTGCTCTGGTGCTCTTTCCAGCTGAAAAGAAGAATGCTGTCCTTTATGAAGGGGATATGGCAGTCACTGAAATCTTCAAGTTTATGGCCGATCATGGAAGTAACTCGGATGATCTTATCAGTGAGAAGGGAAGCTTATGGACTGTAGCAAAAAAGTGGGGAAGGAATCAAAATTTATTTAAGGTCCAGTCTTCTGACATTCACGAGGGAGCTCCCTTTGAAAAGGACACACTTCATGAAATACTTTTAACACAGACGCATAAACAAGGCATTAGAGACAATCAGCCCGAATCACACACATCACAAGGTTTGCAGGAAGCAGCCCTGCATGTGGTGACCGGTTCCACTTTAGTTGCTACTGATAAACTTCTTACTGTACATCCCTTTGATAAATCAGAGGTCCTTATCGTCAAGGCAGATCGAGTTTCTGGATTTGAAGGTCTGATTATTAACAAGAATATCAAATGGGATGTTCTTCTTGAATTGGACAAAGGGCTGGAGATGCTATCTGAGGCTCCTCTGTCTTTTGGAGGACCACTTATAAAGGTTGGAATGCCTCTTGTCGCATTAACTCGAAGATTTGTTATGAATGAGTATCCGGAAGTCCTGCCGGGTGTTTACTTTCTTGATCAATCAGCAACATTTCGTAAAGTCGAAGAGATCAAGTTAGGAAATCAATCTGTCAGCGACTACTGGTTTTTCTATGGGTATTCAAGTTGGGGTTGGGACCAACTCTTTGATGAGATTGCTGAAGGAGCTTGGAACTTGAGTGATGATGGTACGAGGCATTTAGATTGGCCATCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF179 Thioredoxin

Protein Analysis

1102

Amino Acids

123.44

Weight (kDa)

5.23

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF179 PF02622 959 - 1090 4.3e-12 AlgH-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3038
AarI CACCTGC 1 cut(s) 2191
Acc36I ACCTGC 2 cut(s) 1102, 2191
AccB7I CCANNNNNTGG 2 cut(s) 948, 2293
AccI GTMKAC 1 cut(s) 1346
AccII CGCG 2 cut(s) 53, 804
AccIII TCCGGA 1 cut(s) 3094
AciI CCGC 9 cut(s) 53, 139, 296, 375, 425, 467, 624, 696, 2355
AclWI GGATC 6 cut(s) 130, 1410, 1638, 1954, 1970, 2365
AcoI YGGCCR 2 cut(s) 2583, 3293
AcsI RAATTY 5 cut(s) 121, 601, 887, 1953, 2668
AcuI CTGAAG 6 cut(s) 724, 1544, 1683, 1830, 2432, 3267
AcyI GRCGYC 1 cut(s) 300
AfiI CCNNNNNNNGG 8 cut(s) 181, 182, 731, 855, 948, 1123, 2293, 2827
AflII CTTAAG 1 cut(s) 1445
AgeI ACCGGT 1 cut(s) 2834
AhdI GACNNNNNGTC 1 cut(s) 1342
AhlI ACTAGT 1 cut(s) 1649
AjnI CCWGG 1 cut(s) 551
AjuI GAANNNNNNNTTGG 2 cut(s) 1415, 1447
AleI CACNNNNGTG 1 cut(s) 2388
Alw21I GWGCWC 3 cut(s) 791, 2511, 2710
Alw26I GTCTC 6 cut(s) 904, 1142, 1233, 1320, 1940, 2766
AlwI GGATC 6 cut(s) 130, 1410, 1638, 1954, 1970, 2365
AlwNI CAGNNNCTG 2 cut(s) 1697, 2564
Ama87I CYCGRG 1 cut(s) 2172
Aor13HI TCCGGA 1 cut(s) 3094
AoxI GGCC 5 cut(s) 402, 1597, 2118, 2583, 3293
ApeKI GCWGC 3 cut(s) 56, 311, 2816
ApoI RAATTY 5 cut(s) 121, 601, 887, 1953, 2668
ArsI GACNNNNNNTTYG 2 cut(s) 3215, 3247
AsiGI ACCGGT 1 cut(s) 2834
Asp700I GAANNNNTTC 3 cut(s) 441, 482, 2097
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
AsuC2I CCSGG 2 cut(s) 981, 3108
AsuHPI GGTGA 6 cut(s) 633, 1978, 2185, 2215, 2402, 2842
AvaI CYCGRG 1 cut(s) 2172
AvaII GGWCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
BaeI ACNNNNGTAYC 2 cut(s) 1638, 1671
BalI TGGCCA 1 cut(s) 3295
BanII GRGCYC 3 cut(s) 791, 1895, 2710
BauI CACGAG 1 cut(s) 2699
BbsI GAAGAC 5 cut(s) 385, 1854, 1993, 2063, 2679
Bbv12I GWGCWC 3 cut(s) 791, 2511, 2710
BbvI GCAGC 3 cut(s) 68, 298, 2828
BccI CCATC 8 cut(s) 569, 1027, 1114, 1904, 2265, 2381, 3266, 3304
BceAI ACGGC 1 cut(s) 103
BcgI CGANNNNNNTGC 2 cut(s) 1853, 1887
BciT130I CCWGG 1 cut(s) 553
BciVI GTATCC 1 cut(s) 3102
BclI TGATCA 2 cut(s) 1567, 3124
BcnI CCSGG 2 cut(s) 981, 3108
BcoDI GTCTC 6 cut(s) 904, 1142, 1233, 1320, 1940, 2766
BcuI ACTAGT 1 cut(s) 1649
BfaI CTAG 3 cut(s) 1152, 1650, 2112
BfmI CTRYAG 3 cut(s) 1406, 1764, 2640
BfrI CTTAAG 1 cut(s) 1445
BfuAI ACCTGC 2 cut(s) 1102, 2191
BfuI GTATCC 1 cut(s) 3102
BglII AGATCT 1 cut(s) 1428
BisI GCNGC 4 cut(s) 54, 57, 312, 2817
BlsI GCNGC 4 cut(s) 55, 58, 313, 2818
BmcAI AGTACT 1 cut(s) 1807
Bme1390I CCNGG 3 cut(s) 553, 981, 3108
Bme18I GGWCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
BmeRI GACNNNNNGTC 1 cut(s) 1342
BmeT110I CYCGRG 1 cut(s) 2172
BmgT120I GGNCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
BmiI GGNNCC 5 cut(s) 571, 1417, 2839, 3012, 3224
BmrFI CCNGG 3 cut(s) 553, 981, 3108
BmsI GCATC 5 cut(s) 903, 965, 1678, 2334, 2988
BpiI GAAGAC 5 cut(s) 385, 1854, 1993, 2063, 2679
BplI GAGNNNNNCTC 2 cut(s) 1241, 1273
BpmI CTGGAG 1 cut(s) 3014
BpuEI CTTGAG 1 cut(s) 3283
BpuMI CCSGG 2 cut(s) 981, 3108
Bsa29I ATCGAT 2 cut(s) 911, 1921
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 904
BsaJI CCNNGG 6 cut(s) 176, 315, 725, 979, 1257, 1962
BsaWI WCCGGW 2 cut(s) 2834, 3094
Bsc4I CCNNNNNNNGG 8 cut(s) 181, 182, 731, 855, 948, 1123, 2293, 2827
Bse118I RCCGGY 1 cut(s) 2834
Bse1I ACTGG 6 cut(s) 942, 1544, 1945, 2040, 2683, 3194
Bse3DI GCAATG 2 cut(s) 1538, 1980
BseAI TCCGGA 1 cut(s) 3094
BseBI CCWGG 1 cut(s) 553
BseCI ATCGAT 2 cut(s) 911, 1921
BseDI CCNNGG 6 cut(s) 176, 315, 725, 979, 1257, 1962
BseGI GGATG 7 cut(s) 153, 980, 2251, 2407, 2611, 2875, 2971
BseLI CCNNNNNNNGG 8 cut(s) 181, 182, 731, 855, 948, 1123, 2293, 2827
BseMI GCAATG 2 cut(s) 1538, 1980
BseMII CTCAG 8 cut(s) 768, 915, 1239, 1331, 1635, 1724, 2019, 2997
BseNI ACTGG 6 cut(s) 942, 1544, 1945, 2040, 2683, 3194
BseRI GAGGAG 4 cut(s) 118, 149, 1022, 3003
BseXI GCAGC 3 cut(s) 68, 298, 2828
BseYI CCCAGC 3 cut(s) 39, 64, 1986
BsgI GTGCAG 3 cut(s) 1184, 1568, 2425
Bsh1236I CGCG 2 cut(s) 53, 804
Bsh1285I CGRYCG 1 cut(s) 197
BshFI GGCC 5 cut(s) 404, 1599, 2120, 2585, 3295
BshTI ACCGGT 1 cut(s) 2834
BshVI ATCGAT 2 cut(s) 911, 1921
BsiEI CGRYCG 1 cut(s) 197
BsiHKAI GWGCWC 3 cut(s) 791, 2511, 2710
BsiHKCI CYCGRG 1 cut(s) 2172
BsiSI CCGG 4 cut(s) 981, 2835, 3095, 3107
BslFI GGGAC 4 cut(s) 439, 749, 2153, 3236
BslI CCNNNNNNNGG 8 cut(s) 181, 182, 731, 855, 948, 1123, 2293, 2827
BsmAI GTCTC 6 cut(s) 904, 1142, 1233, 1320, 1940, 2766
BsmFI GGGAC 4 cut(s) 439, 749, 2153, 3236
BsmI GAATGC 2 cut(s) 2536, 3054
BsnI GGCC 5 cut(s) 404, 1599, 2120, 2585, 3295
Bso31I GGTCTC 1 cut(s) 904
BsoBI CYCGRG 1 cut(s) 2172
Bsp1286I GDGCHC 4 cut(s) 791, 1895, 2511, 2710
Bsp13I TCCGGA 1 cut(s) 3094
Bsp1407I TGTACA 2 cut(s) 270, 2872
Bsp19I CCATGG 1 cut(s) 176
Bsp68I TCGCGA 1 cut(s) 804
BspACI CCGC 9 cut(s) 53, 139, 296, 375, 425, 467, 624, 696, 2355
BspANI GGCC 5 cut(s) 404, 1599, 2120, 2585, 3295
BspCNI CTCAG 8 cut(s) 769, 914, 1240, 1330, 1634, 1723, 2018, 2998
BspDI ATCGAT 2 cut(s) 911, 1921
BspEI TCCGGA 1 cut(s) 3094
BspFNI CGCG 2 cut(s) 53, 804
BspHI TCATGA 3 cut(s) 201, 1353, 2731
BspLI GGNNCC 5 cut(s) 571, 1417, 2839, 3012, 3224
BspMAI CTGCAG 1 cut(s) 1768
BspMI ACCTGC 2 cut(s) 1102, 2191
BspPI GGATC 6 cut(s) 130, 1410, 1638, 1954, 1970, 2365
BspQI GCTCTTC 2 cut(s) 432, 1659
BspTI CTTAAG 1 cut(s) 1445
BspTNI GGTCTC 1 cut(s) 904
BsrDI GCAATG 2 cut(s) 1538, 1980
BsrFI RCCGGY 1 cut(s) 2834
BsrGI TGTACA 2 cut(s) 270, 2872
BsrI ACTGG 6 cut(s) 942, 1544, 1945, 2040, 2683, 3194
BssAI RCCGGY 1 cut(s) 2834
BssECI CCNNGG 6 cut(s) 176, 315, 725, 979, 1257, 1962
BssNI GRCGYC 1 cut(s) 300
BssSI CACGAG 1 cut(s) 2699
BssT1I CCWWGG 3 cut(s) 176, 725, 1962
Bst2BI CACGAG 1 cut(s) 2699
Bst2UI CCWGG 1 cut(s) 553
Bst6I CTCTTC 5 cut(s) 432, 1659, 1716, 2004, 3147
BstACI GRCGYC 1 cut(s) 300
BstAFI CTTAAG 1 cut(s) 1445
BstAUI TGTACA 2 cut(s) 270, 2872
BstC8I GCNNGC 5 cut(s) 37, 549, 1194, 1475, 1479
BstDSI CCRYGG 2 cut(s) 176, 315
BstEII GGTNACC 2 cut(s) 223, 2830
BstF5I GGATG 7 cut(s) 153, 980, 2251, 2407, 2611, 2875, 2971
BstFNI CGCG 2 cut(s) 53, 804
BstHHI GCGC 1 cut(s) 391
BstMAI GTCTC 6 cut(s) 904, 1142, 1233, 1320, 1940, 2766
BstMCI CGRYCG 1 cut(s) 197
BstMWI GCNNNNNNNGC 6 cut(s) 65, 308, 320, 1198, 2998, 3251
BstNI CCWGG 1 cut(s) 553
BstNSI RCATGY 1 cut(s) 2828
BstPI GGTNACC 2 cut(s) 223, 2830
BstSCI CCNGG 3 cut(s) 551, 979, 3106
BstSFI CTRYAG 3 cut(s) 1406, 1764, 2640
BstUI CGCG 2 cut(s) 53, 804
BstV1I GCAGC 3 cut(s) 68, 298, 2828
BstV2I GAAGAC 5 cut(s) 385, 1854, 1993, 2063, 2679
BstX2I RGATCY 3 cut(s) 135, 1402, 1428
BstXI CCANNNNNNTGG 1 cut(s) 1226
BstYI RGATCY 3 cut(s) 135, 1402, 1428
Bsu15I ATCGAT 2 cut(s) 911, 1921
BsuI GTATCC 1 cut(s) 3102
BsuRI GGCC 5 cut(s) 404, 1599, 2120, 2585, 3295
BsuTUI ATCGAT 2 cut(s) 911, 1921
BtgI CCRYGG 2 cut(s) 176, 315
BtsCI GGATG 7 cut(s) 153, 980, 2251, 2407, 2611, 2875, 2971
BtsI GCAGTG 2 cut(s) 1196, 2464
BtuMI TCGCGA 1 cut(s) 804
BveI ACCTGC 2 cut(s) 1102, 2191
Cac8I GCNNGC 5 cut(s) 37, 549, 1194, 1475, 1479
CaiI CAGNNNCTG 2 cut(s) 1697, 2564
CciI TCATGA 3 cut(s) 201, 1353, 2731
CfoI GCGC 1 cut(s) 391
Cfr10I RCCGGY 1 cut(s) 2834
Cfr13I GGNCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
ClaI ATCGAT 2 cut(s) 911, 1921
CseI GACGC 3 cut(s) 308, 2240, 2761
CspAI ACCGGT 1 cut(s) 2834
CspCI CAANNNNNGTGG 2 cut(s) 2830, 2865
CviAII CATG 8 cut(s) 177, 202, 948, 1354, 1516, 2591, 2732, 2825
DriI GACNNNNNGTC 1 cut(s) 1342
EaeI YGGCCR 2 cut(s) 2583, 3293
Eam1104I CTCTTC 5 cut(s) 432, 1659, 1716, 2004, 3147
Eam1105I GACNNNNNGTC 1 cut(s) 1342
EarI CTCTTC 5 cut(s) 432, 1659, 1716, 2004, 3147
EciI GGCGGA 3 cut(s) 128, 311, 390
Ecl136II GAGCTC 2 cut(s) 789, 2708
Eco130I CCWWGG 3 cut(s) 176, 725, 1962
Eco147I AGGCCT 1 cut(s) 1599
Eco24I GRGCYC 3 cut(s) 791, 1895, 2710
Eco31I GGTCTC 1 cut(s) 904
Eco32I GATATC 2 cut(s) 1298, 1438
Eco47I GGWCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
Eco53kI GAGCTC 2 cut(s) 789, 2708
Eco57I CTGAAG 6 cut(s) 724, 1544, 1683, 1830, 2432, 3267
Eco88I CYCGRG 1 cut(s) 2172
Eco91I GGTNACC 2 cut(s) 223, 2830
EcoICRI GAGCTC 2 cut(s) 789, 2708
EcoO109I RGGNCCY 2 cut(s) 1012, 2896
EcoO65I GGTNACC 2 cut(s) 223, 2830
EcoRII CCWGG 1 cut(s) 551
EcoRV GATATC 2 cut(s) 1298, 1438
EcoT14I CCWWGG 3 cut(s) 176, 725, 1962
EcoT38I GRGCYC 3 cut(s) 791, 1895, 2710
ErhI CCWWGG 3 cut(s) 176, 725, 1962
FaeI CATG 8 cut(s) 180, 205, 951, 1357, 1519, 2594, 2735, 2828
FalI AAGNNNNNCTT 8 cut(s) 1415, 1447, 1742, 1774, 2234, 2266, 2615, 2647
FaqI GGGAC 4 cut(s) 439, 749, 2153, 3236
FatI CATG 8 cut(s) 176, 201, 947, 1353, 1515, 2590, 2731, 2824
FauNDI CATATG 1 cut(s) 1735
FbaI TGATCA 2 cut(s) 1567, 3124
FblI GTMKAC 1 cut(s) 1346
Fnu4HI GCNGC 4 cut(s) 54, 57, 312, 2817
FokI GGATG 7 cut(s) 140, 987, 2258, 2414, 2618, 2862, 2978
FriOI GRGCYC 3 cut(s) 791, 1895, 2710
Fsp4HI GCNGC 4 cut(s) 54, 57, 312, 2817
FspBI CTAG 3 cut(s) 1152, 1650, 2112
GlaI GCGC 1 cut(s) 390
GluI GCNGC 4 cut(s) 54, 57, 312, 2817
GsaI CCCAGC 3 cut(s) 43, 68, 1990
GsuI CTGGAG 1 cut(s) 3014
HaeIII GGCC 5 cut(s) 404, 1599, 2120, 2585, 3295
HapII CCGG 4 cut(s) 981, 2835, 3095, 3107
HgaI GACGC 3 cut(s) 308, 2240, 2761
HhaI GCGC 1 cut(s) 391
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 8 cut(s) 180, 205, 951, 1357, 1519, 2594, 2735, 2828
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HincII GTYRAC 2 cut(s) 954, 1618
HindII GTYRAC 2 cut(s) 954, 1618
HindIII AAGCTT 4 cut(s) 504, 1275, 2414, 2629
HpaI GTTAAC 1 cut(s) 1618
HpaII CCGG 4 cut(s) 981, 2835, 3095, 3107
HphI GGTGA 6 cut(s) 633, 1978, 2185, 2215, 2402, 2842
Hpy166II GTNNAC 6 cut(s) 843, 954, 1347, 1618, 1775, 3115
Hpy8I GTNNAC 6 cut(s) 843, 954, 1347, 1618, 1775, 3115
Hpy99I CGWCG 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 1343
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 308, 320, 1198, 2998, 3251
HpySE526I ACGT 1 cut(s) 1343
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 8 cut(s) 180, 205, 951, 1357, 1519, 2594, 2735, 2828
HspAI GCGC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 3094
Ksp22I TGATCA 2 cut(s) 1567, 3124
KspAI GTTAAC 1 cut(s) 1618
LguI GCTCTTC 2 cut(s) 432, 1659
LmnI GCTCC 7 cut(s) 308, 414, 1861, 2705, 2713, 3016, 3251
Lsp1109I GCAGC 3 cut(s) 68, 298, 2828
LweI GCATC 5 cut(s) 903, 965, 1678, 2334, 2988
MaeI CTAG 3 cut(s) 1152, 1650, 2112
MaeII ACGT 1 cut(s) 1343
MfeI CAATTG 1 cut(s) 1634
MflI RGATCY 3 cut(s) 135, 1402, 1428
MhlI GDGCHC 4 cut(s) 791, 1895, 2511, 2710
MlsI TGGCCA 1 cut(s) 3295
MluNI TGGCCA 1 cut(s) 3295
MlyI GAGTC 2 cut(s) 782, 818
MmeI TCCRAC 4 cut(s) 409, 1038, 1522, 3025
Mox20I TGGCCA 1 cut(s) 3295
MroI TCCGGA 1 cut(s) 3094
MroXI GAANNNNTTC 3 cut(s) 441, 482, 2097
MscI TGGCCA 1 cut(s) 3295
MslI CAYNNNNRTG 1 cut(s) 2388
Msp20I TGGCCA 1 cut(s) 3295
MspA1I CMGCKG 3 cut(s) 624, 817, 2519
MspCI CTTAAG 1 cut(s) 1445
MspI CCGG 4 cut(s) 981, 2835, 3095, 3107
MspR9I CCNGG 3 cut(s) 553, 981, 3108
MunI CAATTG 1 cut(s) 1634
Mva1269I GAATGC 2 cut(s) 2536, 3054
MvaI CCWGG 1 cut(s) 553
MvnI CGCG 2 cut(s) 53, 804
MwoI GCNNNNNNNGC 6 cut(s) 65, 308, 320, 1198, 2998, 3251
NciI CCSGG 2 cut(s) 981, 3108
NcoI CCATGG 1 cut(s) 176
NdeI CATATG 1 cut(s) 1735
NlaIII CATG 8 cut(s) 180, 205, 951, 1357, 1519, 2594, 2735, 2828
NlaIV GGNNCC 5 cut(s) 571, 1417, 2839, 3012, 3224
NmuCI GTSAC 7 cut(s) 166, 1378, 1603, 1687, 2390, 2560, 2830
NruI TCGCGA 1 cut(s) 804
NspI RCATGY 1 cut(s) 2828
OliI CACNNNNGTG 1 cut(s) 2388
PaeR7I CTCGAG 1 cut(s) 2172
PagI TCATGA 3 cut(s) 201, 1353, 2731
PaqCI CACCTGC 1 cut(s) 2191
PceI AGGCCT 1 cut(s) 1599
PciSI GCTCTTC 2 cut(s) 432, 1659
PcsI WCGNNNNNNNCGW 2 cut(s) 93, 413
PctI GAATGC 2 cut(s) 2536, 3054
PdmI GAANNNNTTC 3 cut(s) 441, 482, 2097
PflMI CCANNNNNTGG 2 cut(s) 948, 2293
PinAI ACCGGT 1 cut(s) 2834
PkrI GCNGC 4 cut(s) 55, 58, 313, 2818
Ple19I CGATCG 1 cut(s) 197
PleI GAGTC 2 cut(s) 781, 818
PpsI GAGTC 2 cut(s) 781, 818
PpuMI RGGWCCY 2 cut(s) 1012, 2896
PsiI TTATAA 1 cut(s) 3038
Psp124BI GAGCTC 2 cut(s) 791, 2710
Psp5II RGGWCCY 2 cut(s) 1012, 2896
Psp6I CCWGG 1 cut(s) 551
PspEI GGTNACC 2 cut(s) 223, 2830
PspFI CCCAGC 3 cut(s) 39, 64, 1986
PspGI CCWGG 1 cut(s) 551
PspN4I GGNNCC 5 cut(s) 571, 1417, 2839, 3012, 3224
PspPI GGNCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
PspPPI RGGWCCY 2 cut(s) 1012, 2896
PspXI VCTCGAGB 1 cut(s) 2172
PstI CTGCAG 1 cut(s) 1768
PstNI CAGNNNCTG 2 cut(s) 1697, 2564
PsuI RGATCY 3 cut(s) 135, 1402, 1428
PvuI CGATCG 1 cut(s) 197
PvuII CAGCTG 2 cut(s) 817, 2519
RruI TCGCGA 1 cut(s) 804
RseI CAYNNNNRTG 1 cut(s) 2388
SacI GAGCTC 2 cut(s) 791, 2710
SapI GCTCTTC 2 cut(s) 432, 1659
SatI GCNGC 4 cut(s) 54, 57, 312, 2817
Sau96I GGNCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
ScaI AGTACT 1 cut(s) 1807
SchI GAGTC 2 cut(s) 782, 818
ScrFI CCNGG 3 cut(s) 553, 981, 3108
SduI GDGCHC 4 cut(s) 791, 1895, 2511, 2710
SfaNI GCATC 5 cut(s) 903, 965, 1678, 2334, 2988
SfcI CTRYAG 3 cut(s) 1406, 1764, 2640
Sfr274I CTCGAG 1 cut(s) 2172
SinI GGWCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
SlaI CTCGAG 1 cut(s) 2172
SmiMI CAYNNNNRTG 1 cut(s) 2388
SmlI CTYRAG 3 cut(s) 1445, 2172, 3262
SmoI CTYRAG 3 cut(s) 1445, 2172, 3262
SpeI ACTAGT 1 cut(s) 1649
SseBI AGGCCT 1 cut(s) 1599
SsiI CCGC 9 cut(s) 53, 139, 296, 375, 425, 467, 624, 696, 2355
SspMI CTAG 3 cut(s) 1152, 1650, 2112
SstI GAGCTC 2 cut(s) 791, 2710
StuI AGGCCT 1 cut(s) 1599
StyD4I CCNGG 3 cut(s) 551, 979, 3106
StyI CCWWGG 3 cut(s) 176, 725, 1962
TaiI ACGT 1 cut(s) 1346
TaqII GACCGA 1 cut(s) 1249
TatI WGTACW 7 cut(s) 270, 342, 944, 963, 1286, 1805, 2872
TauI GCSGC 1 cut(s) 56
TseFI GTSAC 7 cut(s) 166, 1378, 1603, 1687, 2390, 2560, 2830
TseI GCWGC 3 cut(s) 56, 311, 2816
Tsp45I GTSAC 7 cut(s) 166, 1378, 1603, 1687, 2390, 2560, 2830
TspGWI ACGGA 4 cut(s) 332, 401, 405, 435
Van91I CCANNNNNTGG 2 cut(s) 948, 2293
Vha464I CTTAAG 1 cut(s) 1445
VpaK11BI GGWCC 8 cut(s) 431, 1012, 1261, 2254, 2680, 2896, 3029, 3223
XapI RAATTY 5 cut(s) 121, 601, 887, 1953, 2668
XceI RCATGY 1 cut(s) 2828
XhoI CTCGAG 1 cut(s) 2172
XmiI GTMKAC 1 cut(s) 1346
XmnI GAANNNNTTC 3 cut(s) 441, 482, 2097
XspI CTAG 3 cut(s) 1152, 1650, 2112
ZrmI AGTACT 1 cut(s) 1807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.