pycom13g00640

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005651
Physical Location & Seq
Reverse (-)
406465 .. 408661
2197 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g00640.1

Sequence Viewer

Length: 1047 bp
ATGGGCTTTTGTTTCACTTCCCAATCGATCAAATTTGAGTTGTCGCCGTTCCGCCTGTTCAATTTGATGGCAATTCCTGTTCTCTCAATGTTTTTGACCAAACTTCTCACCGAGGATTTACCTAGATTATTTGTACGCCCAAAAAAGATTGTCTTGGATTTCCAGAAAGGAAAAGCAGTTGGCCCTGTCGGAATTGATTTCAAGTCAGGGGACATACAAGAAGGAAACAAGGATTTTGTTGGAGAATTATCAGTGACCCTTGTAGATGCTCGGAAGCTTTCCTATGTTTTCTATGGCAAAACAGATCCCTATGTTACACTAAGCCTGGGAGACCAAATTATACGCAGTAAAAAAAACAGTCAAACTACTGTTATTGGACCTCCTGGTGAGCCGATCTGGAATCAGGATTTTGATATACTTGTGGCGAACCCTAAAAAACAAAAATTATACATCGAAGTCAAGGATTCTCTTGGATTCACAGACTTAACTATTGGTACAGGAGAGGTTGATCTGGGATCTCTCCAAGACACTGTACCAACGGACAGGATTGTGGTACTGCAAGGAGGTTGGGGCCTGTTTAAAACGAAATCTGCTGGTGAAATACTACTTCGATTGACATATAAGGCATATGTTGAGGATGAAGAGGATGACAAAATTCAGGTGGCTTCTATGGATACTGACGCTTCAGATTCAGATGATGAGTTGTCTGATTCTGTGAAGGATAAACTTGAGCCTGGAAATGAAGCAGACAAAGAGTCCTTCATGGACGTTCTAGCAGCTTTAATTGTGAGTGAAGAATTTCAAGGGATAGTGGCATCCGAAACAGGAAACGCCAAACTTGGGGATGATATCTCAATTGCAGGGTCAAAATTTTCAAAATTGCGGCGTGGACTTGATACTGAATCTGTGCCTTCAAATTCCAATAATAATTCGGAAGGCTCTCAAGGAGTATCCGTGGAAACCACCTTACTTTGGCTTTCTTTAGTTGCTGGCATGTCAGTGCTAATTGCAGTCAACATTGGTGGCTCAAACATTTTCAATCCTTGA

Protein Analysis

349

Amino Acids

37.9

Weight (kDa)

4.57

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 81 - 179 2.9e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 52, 883
AclWI GGATC 2 cut(s) 299, 523
AcsI RAATTY 5 cut(s) 32, 654, 797, 869, 916
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 4 cut(s) 135, 496, 534, 555
AfiI CCNNNNNNNGG 2 cut(s) 840, 972
AgsI TTSAA 6 cut(s) 61, 202, 803, 876, 915, 1039
AhdI GACNNNNNGTC 1 cut(s) 754
AjnI CCWGG 3 cut(s) 324, 382, 733
AluBI AGCT 2 cut(s) 277, 779
AluI AGCT 2 cut(s) 277, 779
Alw26I GTCTC 1 cut(s) 324
AlwI GGATC 2 cut(s) 299, 523
AoxI GGCC 2 cut(s) 181, 571
ApeKI GCWGC 1 cut(s) 776
ApoI RAATTY 5 cut(s) 32, 654, 797, 869, 916
Asp700I GAANNNNTTC 1 cut(s) 798
AspS9I GGNCC 3 cut(s) 182, 377, 571
AsuHPI GGTGA 3 cut(s) 100, 398, 608
AvaII GGWCC 1 cut(s) 377
BbvI GCAGC 1 cut(s) 788
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 31
BciT130I CCWGG 3 cut(s) 326, 384, 735
BciVI GTATCC 2 cut(s) 667, 961
BcoDI GTCTC 1 cut(s) 324
BfaI CTAG 2 cut(s) 123, 773
BfuI GTATCC 2 cut(s) 667, 961
BisI GCNGC 2 cut(s) 777, 884
BlsI GCNGC 2 cut(s) 778, 885
Bme1390I CCNGG 3 cut(s) 326, 384, 735
Bme18I GGWCC 1 cut(s) 377
BmeRI GACNNNNNGTC 1 cut(s) 754
BmgT120I GGNCC 3 cut(s) 182, 377, 571
BmiI GGNNCC 1 cut(s) 572
BmrFI CCNGG 3 cut(s) 326, 384, 735
BmsI GCATC 2 cut(s) 256, 824
BpuEI CTTGAG 2 cut(s) 749, 927
Bsa29I ATCGAT 1 cut(s) 26
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 111, 325, 954
BsaXI ACNNNNNCTCC 2 cut(s) 939, 969
Bsc4I CCNNNNNNNGG 2 cut(s) 840, 972
BseBI CCWGG 3 cut(s) 326, 384, 735
BseCI ATCGAT 1 cut(s) 26
BseDI CCNNGG 3 cut(s) 111, 325, 954
BseGI GGATG 4 cut(s) 643, 652, 815, 850
BseLI CCNNNNNNNGG 2 cut(s) 840, 972
BseXI GCAGC 1 cut(s) 788
BshFI GGCC 2 cut(s) 183, 573
BshVI ATCGAT 1 cut(s) 26
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 2 cut(s) 840, 972
BsmAI GTCTC 1 cut(s) 324
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 2 cut(s) 183, 573
Bso31I GGTCTC 1 cut(s) 324
Bsp143I GATC 5 cut(s) 27, 304, 393, 508, 515
BspACI CCGC 2 cut(s) 52, 883
BspANI GGCC 2 cut(s) 183, 573
BspDI ATCGAT 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 572
BspPI GGATC 2 cut(s) 299, 523
BspTNI GGTCTC 1 cut(s) 324
BssECI CCNNGG 3 cut(s) 111, 325, 954
BssMI GATC 5 cut(s) 27, 304, 393, 508, 515
Bst2UI CCWGG 3 cut(s) 326, 384, 735
Bst4CI ACNGT 3 cut(s) 359, 370, 532
Bst6I CTCTTC 1 cut(s) 636
BstC8I GCNNGC 1 cut(s) 991
BstDEI CTNAG 1 cut(s) 320
BstDSI CCRYGG 1 cut(s) 954
BstF5I GGATG 4 cut(s) 643, 652, 815, 850
BstKTI GATC 5 cut(s) 30, 307, 396, 511, 518
BstMAI GTCTC 1 cut(s) 324
BstMBI GATC 5 cut(s) 27, 304, 393, 508, 515
BstNI CCWGG 3 cut(s) 326, 384, 735
BstNSI RCATGY 1 cut(s) 997
BstSCI CCNGG 3 cut(s) 324, 382, 733
BstV1I GCAGC 1 cut(s) 788
BstX2I RGATCY 2 cut(s) 304, 515
BstYI RGATCY 2 cut(s) 304, 515
Bsu15I ATCGAT 1 cut(s) 26
BsuI GTATCC 2 cut(s) 667, 961
BsuRI GGCC 2 cut(s) 183, 573
BsuTUI ATCGAT 1 cut(s) 26
BtgI CCRYGG 1 cut(s) 954
BtsCI GGATG 4 cut(s) 643, 652, 815, 850
BtsIMutI CAGTG 3 cut(s) 258, 528, 1005
Cac8I GCNNGC 1 cut(s) 991
Cfr13I GGNCC 3 cut(s) 182, 377, 571
ClaI ATCGAT 1 cut(s) 26
CseI GACGC 1 cut(s) 689
Csp6I GTAC 4 cut(s) 134, 495, 533, 554
CspCI CAANNNNNGTGG 2 cut(s) 1003, 1038
CviAII CATG 2 cut(s) 763, 994
CviQI GTAC 4 cut(s) 134, 495, 533, 554
DdeI CTNAG 1 cut(s) 320
DpnI GATC 5 cut(s) 29, 306, 395, 510, 517
DpnII GATC 5 cut(s) 27, 304, 393, 508, 515
DraI TTTAAA 1 cut(s) 580
DriI GACNNNNNGTC 1 cut(s) 754
Eam1104I CTCTTC 1 cut(s) 636
Eam1105I GACNNNNNGTC 1 cut(s) 754
EarI CTCTTC 1 cut(s) 636
EciI GGCGGA 1 cut(s) 41
Eco31I GGTCTC 1 cut(s) 324
Eco32I GATATC 1 cut(s) 850
Eco47I GGWCC 1 cut(s) 377
Eco57I CTGAAG 1 cut(s) 669
EcoO109I RGGNCCY 1 cut(s) 571
EcoRII CCWGG 3 cut(s) 324, 382, 733
EcoRV GATATC 1 cut(s) 850
FaeI CATG 2 cut(s) 766, 997
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FaqI GGGAC 1 cut(s) 224
FatI CATG 2 cut(s) 762, 993
FauNDI CATATG 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 777, 884
FokI GGATG 4 cut(s) 650, 659, 802, 857
Fsp4HI GCNGC 2 cut(s) 777, 884
FspBI CTAG 2 cut(s) 123, 773
GluI GCNGC 2 cut(s) 777, 884
HaeIII GGCC 2 cut(s) 183, 573
HgaI GACGC 1 cut(s) 689
Hin1II CATG 2 cut(s) 766, 997
HincII GTYRAC 1 cut(s) 1015
HindII GTYRAC 1 cut(s) 1015
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 7 cut(s) 400, 464, 474, 689, 710, 755, 902
HphI GGTGA 3 cut(s) 100, 398, 608
Hpy166II GTNNAC 2 cut(s) 890, 1015
Hpy188I TCNGA 7 cut(s) 191, 273, 688, 694, 709, 820, 934
Hpy188III TCNNGA 3 cut(s) 163, 397, 404
Hpy8I GTNNAC 2 cut(s) 890, 1015
HpyAV CCTTC 5 cut(s) 215, 712, 769, 921, 929
HpyCH4III ACNGT 3 cut(s) 359, 370, 532
HpyCH4IV ACGT 1 cut(s) 768
HpyCH4V TGCA 3 cut(s) 559, 860, 1010
HpyF3I CTNAG 1 cut(s) 320
HpySE526I ACGT 1 cut(s) 768
Hsp92II CATG 2 cut(s) 766, 997
Kzo9I GATC 5 cut(s) 27, 304, 393, 508, 515
Lsp1109I GCAGC 1 cut(s) 788
LweI GCATC 2 cut(s) 256, 824
MaeI CTAG 2 cut(s) 123, 773
MaeII ACGT 1 cut(s) 768
MaeIII GTNAC 2 cut(s) 253, 313
MalI GATC 5 cut(s) 29, 306, 395, 510, 517
MboI GATC 5 cut(s) 27, 304, 393, 508, 515
MboII GAAGA 2 cut(s) 653, 806
MfeI CAATTG 1 cut(s) 855
MflI RGATCY 2 cut(s) 304, 515
MlyI GAGTC 1 cut(s) 764
MmeI TCCRAC 2 cut(s) 169, 220
MnlI CCTC 6 cut(s) 106, 390, 496, 557, 628, 637
MroXI GAANNNNTTC 1 cut(s) 798
MseI TTAA 3 cut(s) 485, 579, 782
MslI CAYNNNNRTG 1 cut(s) 998
MspR9I CCNGG 3 cut(s) 326, 384, 735
MunI CAATTG 1 cut(s) 855
MvaI CCWGG 3 cut(s) 326, 384, 735
NdeI CATATG 1 cut(s) 628
NdeII GATC 5 cut(s) 27, 304, 393, 508, 515
NlaIII CATG 2 cut(s) 766, 997
NlaIV GGNNCC 1 cut(s) 572
NmuCI GTSAC 1 cut(s) 253
NspI RCATGY 1 cut(s) 997
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 6 cut(s) 400, 464, 474, 689, 710, 902
PkrI GCNGC 2 cut(s) 778, 885
PleI GAGTC 1 cut(s) 763
PpsI GAGTC 1 cut(s) 763
Psp6I CCWGG 3 cut(s) 324, 382, 733
PspGI CCWGG 3 cut(s) 324, 382, 733
PspN4I GGNNCC 1 cut(s) 572
PspPI GGNCC 3 cut(s) 182, 377, 571
PsuI RGATCY 2 cut(s) 304, 515
RsaI GTAC 4 cut(s) 135, 496, 534, 555
RsaNI GTAC 4 cut(s) 134, 495, 533, 554
RseI CAYNNNNRTG 1 cut(s) 998
SaqAI TTAA 3 cut(s) 485, 579, 782
SatI GCNGC 2 cut(s) 777, 884
Sau3AI GATC 5 cut(s) 27, 304, 393, 508, 515
Sau96I GGNCC 3 cut(s) 182, 377, 571
SchI GAGTC 1 cut(s) 764
ScrFI CCNGG 3 cut(s) 326, 384, 735
SetI ASST 9 cut(s) 124, 279, 382, 507, 568, 663, 771, 781, 968
SfaNI GCATC 2 cut(s) 256, 824
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 998
SmlI CTYRAG 2 cut(s) 728, 942
SmoI CTYRAG 2 cut(s) 728, 942
SsiI CCGC 2 cut(s) 52, 883
SspMI CTAG 2 cut(s) 123, 773
StyD4I CCNGG 3 cut(s) 324, 382, 733
TaaI ACNGT 3 cut(s) 359, 370, 532
TaiI ACGT 1 cut(s) 771
TaqI TCGA 3 cut(s) 26, 453, 610
TauI GCSGC 1 cut(s) 886
TfiI GAWTC 6 cut(s) 400, 464, 474, 689, 710, 902
Tru1I TTAA 3 cut(s) 485, 579, 782
Tru9I TTAA 3 cut(s) 485, 579, 782
TscAI CASTG 3 cut(s) 258, 535, 1005
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 1 cut(s) 776
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 3 cut(s) 654, 751, 756
TspGWI ACGGA 2 cut(s) 554, 943
TspRI CASTG 3 cut(s) 258, 535, 1005
VpaK11BI GGWCC 1 cut(s) 377
XapI RAATTY 5 cut(s) 32, 654, 797, 869, 916
XceI RCATGY 1 cut(s) 997
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 2 cut(s) 123, 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.