pycom13g05330

Cytochrome b5-like Heme/Steroid binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012398
Physical Location & Seq
Forward (+)
3562188 .. 3563700
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 405 bp
ATGCCGACGTTGACGAAGCTGTACACAATGCAAGAAGCGTCGCAGCACAACACCAAAGACAACTGCTGGGTCGTCATCGATGGCAAGGTGTACGATGTGTCAACCTATTTGGACGACCACCCTGGTGGAGACGATGTGCTCCTGGCTGCAACTGGAAGAGATGCCACCGAAGATTTTGAAGATGCCGGGCACAGCAAAACTGCGAGGGAGGAAATGGAAGCCTTCTGCATTGGGGAGCTTGACACCACCTCCTCGGATATTCCAGAGCTCGAGATTTTCTCCAAGAATCAGCCAACCGATTATCCAAAGAAGCTCGTGGACTTGACAAAGCAATACTGGGCTGTTCCTGCGGCTGTTGTTAGCATCTCCGTGGTTGTCGCGGTCTTGCACTTGCGCAAGAATTAA

Protein Analysis

135

Amino Acids

14.88

Weight (kDa)

4.58

Isoelectric Point (pI)

28.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 81 4.7e-28 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26340
fragaria_vesca FvH4_4g29701
malus_domestica MD13G1059800.v1.1 MD13G1059900.v1.1 MD16G1059300.v1.1
prunus_persica Prupe.1G289700_v2.0.a1
pyrus_communis pycom13g05330
rosa_chinensis RchiOBHm_Chr4g0437951
rosa_laevigata RLG00000006399
rosa_multiflora Rmu_sc0007538.1_g000013 Rmu_sc0025141.1_g000004
rosa_roxburghii Rroxscaffold_5G00379110
rosa_rugosa Rorug04G0305800
rosa_samantha Rh4AG357400 Rh4BG368300 Rh4CG382400 Rh4DG363200
rosa_wichuraiana Rw0G011530 Rw0G016140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 395
AccII CGCG 1 cut(s) 380
AciI CCGC 2 cut(s) 350, 380
AfaI GTAC 2 cut(s) 23, 92
AgsI TTSAA 1 cut(s) 179
AjnI CCWGG 2 cut(s) 121, 141
AleI CACNNNNGTG 1 cut(s) 123
AluBI AGCT 4 cut(s) 19, 238, 268, 313
AluI AGCT 4 cut(s) 19, 238, 268, 313
Alw21I GWGCWC 2 cut(s) 141, 270
Alw26I GTCTC 1 cut(s) 123
Ama87I CYCGRG 1 cut(s) 269
ApeKI GCWGC 2 cut(s) 43, 146
AspLEI GCGC 1 cut(s) 396
AsuC2I CCSGG 1 cut(s) 187
AvaI CYCGRG 1 cut(s) 269
BaeGI GKGCMC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 270
BauI CACGAG 1 cut(s) 314
Bbv12I GWGCWC 2 cut(s) 141, 270
BbvI GCAGC 2 cut(s) 55, 133
BccI CCATC 1 cut(s) 74
BciT130I CCWGG 2 cut(s) 123, 143
BcnI CCSGG 1 cut(s) 187
BcoDI GTCTC 1 cut(s) 123
BisI GCNGC 3 cut(s) 44, 147, 351
BlsI GCNGC 3 cut(s) 45, 148, 352
Bme1390I CCNGG 3 cut(s) 123, 143, 187
BmeT110I CYCGRG 1 cut(s) 269
BmrFI CCNGG 3 cut(s) 123, 143, 187
BmrI ACTGGG 1 cut(s) 346
BmsI GCATC 3 cut(s) 151, 172, 372
BmuI ACTGGG 1 cut(s) 346
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpuMI CCSGG 1 cut(s) 187
Bsa29I ATCGAT 1 cut(s) 78
BsaJI CCNNGG 3 cut(s) 121, 252, 369
BsaXI ACNNNNNCTCC 4 cut(s) 120, 150, 233, 263
Bse1I ACTGG 2 cut(s) 157, 341
BseBI CCWGG 2 cut(s) 123, 143
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 3 cut(s) 121, 252, 369
BseNI ACTGG 2 cut(s) 157, 341
BseRI GAGGAG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 2 cut(s) 55, 133
BseYI CCCAGC 1 cut(s) 66
Bsh1236I CGCG 1 cut(s) 380
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 141, 270
BsiHKCI CYCGRG 1 cut(s) 269
BsiSI CCGG 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 123
BsmBI CGTCTC 1 cut(s) 123
BsoBI CYCGRG 1 cut(s) 269
Bsp1286I GDGCHC 3 cut(s) 141, 192, 270
Bsp1407I TGTACA 1 cut(s) 21
BspACI CCGC 2 cut(s) 350, 380
BspDI ATCGAT 1 cut(s) 78
BspFNI CGCG 1 cut(s) 380
BsrGI TGTACA 1 cut(s) 21
BsrI ACTGG 2 cut(s) 157, 341
BssECI CCNNGG 3 cut(s) 121, 252, 369
BssSI CACGAG 1 cut(s) 314
Bst2BI CACGAG 1 cut(s) 314
Bst2UI CCWGG 2 cut(s) 123, 143
Bst6I CTCTTC 1 cut(s) 151
BstAUI TGTACA 1 cut(s) 21
BstDSI CCRYGG 1 cut(s) 369
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 1 cut(s) 396
BstMAI GTCTC 1 cut(s) 123
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstNI CCWGG 2 cut(s) 123, 143
BstSCI CCNGG 3 cut(s) 121, 141, 185
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 1 cut(s) 380
BstV1I GCAGC 2 cut(s) 55, 133
BstXI CCANNNNNNTGG 1 cut(s) 125
Bsu15I ATCGAT 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 78
BtgI CCRYGG 1 cut(s) 369
CfoI GCGC 1 cut(s) 396
ClaI ATCGAT 1 cut(s) 78
CseI GACGC 1 cut(s) 27
Csp6I GTAC 2 cut(s) 22, 91
CviJI RGCY 9 cut(s) 19, 146, 221, 238, 268, 292, 313, 341, 353
CviKI_1 RGCY 9 cut(s) 19, 146, 221, 238, 268, 292, 313, 341, 353
CviQI GTAC 2 cut(s) 22, 91
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco53kI GAGCTC 1 cut(s) 268
Eco88I CYCGRG 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 268
EcoRII CCWGG 2 cut(s) 121, 141
EcoT38I GRGCYC 1 cut(s) 270
Esp3I CGTCTC 1 cut(s) 123
Fnu4HI GCNGC 3 cut(s) 44, 147, 351
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 3 cut(s) 44, 147, 351
FspI TGCGCA 1 cut(s) 395
GlaI GCGC 1 cut(s) 395
GluI GCNGC 3 cut(s) 44, 147, 351
GsaI CCCAGC 1 cut(s) 70
HapII CCGG 1 cut(s) 186
HgaI GACGC 1 cut(s) 27
HhaI GCGC 1 cut(s) 396
Hin6I GCGC 1 cut(s) 394
HinP1I GCGC 1 cut(s) 394
HincII GTYRAC 2 cut(s) 12, 102
HindII GTYRAC 2 cut(s) 12, 102
HinfI GANTC 1 cut(s) 286
HpaII CCGG 1 cut(s) 186
Hpy166II GTNNAC 5 cut(s) 12, 24, 91, 102, 319
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 2 cut(s) 263, 271
Hpy8I GTNNAC 5 cut(s) 12, 24, 91, 102, 319
Hpy99I CGWCG 2 cut(s) 10, 43
HpyAV CCTTC 1 cut(s) 232
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 4 cut(s) 31, 149, 228, 388
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 8
HspAI GCGC 1 cut(s) 394
LmnI GCTCC 2 cut(s) 144, 235
Lsp1109I GCAGC 2 cut(s) 55, 133
LweI GCATC 3 cut(s) 151, 172, 372
MaeII ACGT 1 cut(s) 8
MboII GAAGA 3 cut(s) 168, 182, 191
MhlI GDGCHC 3 cut(s) 141, 192, 270
MluCI AATT 1 cut(s) 400
MnlI CCTC 4 cut(s) 198, 202, 259, 262
MseI TTAA 1 cut(s) 403
MslI CAYNNNNRTG 2 cut(s) 123, 368
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 3 cut(s) 123, 143, 187
MvaI CCWGG 2 cut(s) 123, 143
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 1 cut(s) 347
NciI CCSGG 1 cut(s) 187
NsbI TGCGCA 1 cut(s) 395
OliI CACNNNNGTG 1 cut(s) 123
PaeR7I CTCGAG 1 cut(s) 269
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 3 cut(s) 45, 148, 352
Psp124BI GAGCTC 1 cut(s) 270
Psp6I CCWGG 2 cut(s) 121, 141
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 2 cut(s) 121, 141
RsaI GTAC 2 cut(s) 23, 92
RsaNI GTAC 2 cut(s) 22, 91
RseI CAYNNNNRTG 2 cut(s) 123, 368
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 403
SatI GCNGC 3 cut(s) 44, 147, 351
ScrFI CCNGG 3 cut(s) 123, 143, 187
SduI GDGCHC 3 cut(s) 141, 192, 270
SetI ASST 8 cut(s) 11, 21, 90, 107, 240, 251, 270, 315
SfaNI GCATC 3 cut(s) 151, 172, 372
Sfr274I CTCGAG 1 cut(s) 269
SlaI CTCGAG 1 cut(s) 269
SmiMI CAYNNNNRTG 2 cut(s) 123, 368
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 1 cut(s) 400
SsiI CCGC 2 cut(s) 350, 380
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 3 cut(s) 121, 141, 185
TaiI ACGT 1 cut(s) 11
TaqI TCGA 2 cut(s) 78, 270
TasI AATT 1 cut(s) 400
TatI WGTACW 1 cut(s) 21
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 1 cut(s) 403
Tru9I TTAA 1 cut(s) 403
TseI GCWGC 2 cut(s) 43, 146
TspGWI ACGGA 1 cut(s) 358
XhoI CTCGAG 1 cut(s) 269
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.