Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0011232

Physical Location & Seq

Genomic Coordinates Forward (+)

3575201 .. 3578109

Gene Structure 2909 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1686 bp

ATGGCGTCTCTTCCTTTCACTCACTTTCTCCTCCTACCCAGGTGCCATTCGGTTTGGCCCAATAGCCGGTCGATGAGTTTCGTGCAGCTTCAGGGTCTTAGATTGCATGGTAAAAACCGTGGACTTGCAGCTCTCCCTAAAAAGATAAACTCAGAGAACTCAGCTCTGGAGGGTTCCGTTGAAGATGAGGAGGTGGTTGTGAAGAAAAAGACATCTAGGACTCCAAGAAGAACCCGAAAGAAAGCAACATCCGATAATGAGTCTTCGGATGAGGAAACTGTCATTTCTGCATCTAGTGAAGAGACCAAGAAACCGCGTGGAAGAACTCGTAAGAAAGATCTAGTTGCATCCGCTTCCACAAGCGTGGAGGAAGAACAAACTGAGAAGAAGGTGAGGAAGAGAAGAACTAAGAAGAAAGACGATGCAATTGAGCAGCAGGTTAGTGACGGTGAACTTAGTGAGACTGAGGATTTTACATTCGTTATGGATATGAAGGATGAGAATGAGGAAGACCTAGAATTGAAAATTGATGAGGGAGAGGATATTAGCGAAACTTATGATTGGCCTCCTCTTGTTTGTTGCTTTGGAGCCGCACAACATGCTTTTGTGCCCTCAGGGAGGCCAGCCAACAGACTTGTAGATTATGAAATACATGAAAGAAAGAAAGATGCGTTATGGGCCCCTGAGAAATTTGTTAGGTCTCCTGGGGGATCTGCAGGCAGCGTCGCAATTGCTCTTGCAAGCTTGGGAGGCAAGGTTGCTTTCATGGGAAAGCTTGGAGATGATCAGTTTGGTCAGGCCATGCTGTATTATATGAATGTCAAAAATGTTCAAACCCGTTCTGTTCGCATAGATACTAAAAGAGCAACTGCAGTATCACAAATGAAGATTGGTAAAAGGGGTCGCATGAAATTGAGTTGTGTCAAACCGTGTGCTGAGGATTCTTTAACAAAGTCAGAGATCAACGTCGATGTGCTGAAGGAGGCAAAGATGTTTTATTTCAGCACAAATTCTCTGCTTGATCGAAACATGAGGTCAACTACACTGCAAGCAATCAAGATTTCGAAGAAACTTGGAGGAGTTATTTTCTACGATGTAAACCTTCCATTGCCACTATGGCAGTCTTGTGAAGAAACAAAGTTGTTCGTACAGCAAGTGTGGAATCTTGCTGATATTATTGAAGTTACTAAGCAAGAGCTTGAGTTTCTCTGTGGGATCCAGCCCTCTGAGGAATTTGACACTAAAAATAATGACAGGTCAAAGTTTGTTCATCATTCACCAGAAGTGGTTGAACAGCTTTGGCATGACAATCTTAAGGTTTTGTTTGTGACAAACGGGACTTCGAAGATACACTACTACACAAAGGACCACCAAGGTGCCGTTAATGGAATGGAAGATCCCCCCATTAGTCCTTTCACTTCTGATATGTCAGCATCTGGAGACGGCATTGTGGCGGCTCTCATGAGAATGTTGACAGTTCAACCACATCTTATTACTGATAAAGAATATCTGGAGCACTCAATCAACTATGCAATCGATTGCGGGGTGATAGATCAATGGCTGCTTGGACAAGAACGCGGCTTCCCTCCGAAGGAAGACATGGAAGAAGTGGTTCCTGATTCAGACGGTATAAGGTCTATAAGAGAAATGGAGTATCGCACTCTCACACCAGAGACTGTAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000427 GO:0000428 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006355 GO:0006725 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009295 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009662 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0030880 GO:0031323 GO:0031326 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0042644 GO:0042646 GO:0042793 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061695 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1903506 GO:1990234 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PfkB

Protein Analysis

562

Amino Acids

63.26

Weight (kDa)

5.8

Isoelectric Point (pI)

50.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PfkB	PF00294	221 - 493	6.5e-32	pfkB family carbohydrate kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011232)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69200
fragaria_vesca	FvH4_4g29720 FvH4_4g29720 FvH4_4g29720 FvH4_4g29720
malus_domestica	MD13G1060100.v1.1
prunus_persica	Prupe.1G289300_v2.0.a1 Prupe.1G289300_v2.0.a1
pyrus_communis	pycom13g05350
rosa_chinensis	RchiOBHm_Chr4g0437971
rosa_laevigata	RLG00000006397
rosa_multiflora	Rmu_sc0007538.1_g000008
rosa_roxburghii	Rroxscaffold_5G00379130
rosa_rugosa	Rorug04G0306000
rosa_samantha	Rh4AG357600 Rh4BG368500 Rh4CG382600 Rh4DG363400
rosa_wichuraiana	Rw0G011510 Rw0G016160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	427
AccB1I	GGYRCC	2 cut(s)	42, 1376
AccII	CGCG	2 cut(s)	316, 1578
AciI	CCGC	6 cut(s)	314, 351, 591, 1454, 1542, 1578
AclWI	GGATC	4 cut(s)	718, 1210, 1223, 1391
AcsI	RAATTY	3 cut(s)	689, 1009, 1232
AcuI	CTGAAG	2 cut(s)	74, 998
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	1149
AfiI	CCNNNNNNNGG	3 cut(s)	66, 618, 1591
AflII	CTTAAG	1 cut(s)	1313
AgsI	TTSAA	6 cut(s)	182, 523, 833, 1181, 1292, 1481
AjnI	CCWGG	2 cut(s)	38, 703
AleI	CACNNNNGTG	2 cut(s)	362, 1374
AloI	GAACNNNNNNTCC	2 cut(s)	1566, 1598
AluBI	AGCT	7 cut(s)	88, 131, 164, 744, 775, 1198, 1297
AluI	AGCT	7 cut(s)	88, 131, 164, 744, 775, 1198, 1297
Alw21I	GWGCWC	1 cut(s)	1518
Alw26I	GTCTC	6 cut(s)	12, 296, 455, 705, 1434, 1667
AlwI	GGATC	4 cut(s)	718, 1210, 1223, 1391
AlwNI	CAGNNNCTG	2 cut(s)	1436, 1676
AoxI	GGCC	5 cut(s)	56, 563, 620, 678, 798
ApaI	GGGCCC	1 cut(s)	682
ApeKI	GCWGC	5 cut(s)	85, 128, 433, 720, 1561
ApoI	RAATTY	3 cut(s)	689, 1009, 1232
Asp700I	GAANNNNTTC	1 cut(s)	1611
AspS9I	GGNCC	4 cut(s)	57, 678, 679, 1366
AsuHPI	GGTGA	4 cut(s)	403, 461, 1269, 1558
AsuII	TTCGAA	2 cut(s)	1064, 1343
AvaII	GGWCC	1 cut(s)	1366
AxyI	CCTNAGG	1 cut(s)	613
BaeGI	GKGCMC	2 cut(s)	612, 682
BaeI	ACNNNNGTAYC	2 cut(s)	858, 891
BamHI	GGATCC	1 cut(s)	1215
BanI	GGYRCC	2 cut(s)	42, 1376
BanII	GRGCYC	1 cut(s)	682
BarI	GAAGNNNNNNTAC	2 cut(s)	1337, 1369
BbsI	GAAGAC	3 cut(s)	255, 516, 1602
Bbv12I	GWGCWC	1 cut(s)	1518
BbvCI	CCTCAGC	1 cut(s)	936
BbvI	GCAGC	5 cut(s)	97, 140, 445, 732, 1548
BceAI	ACGGC	2 cut(s)	1364, 1459
BciT130I	CCWGG	2 cut(s)	40, 705
BclI	TGATCA	1 cut(s)	784
BcoDI	GTCTC	6 cut(s)	12, 296, 455, 705, 1434, 1667
BfaI	CTAG	4 cut(s)	216, 294, 341, 515
BfmI	CTRYAG	2 cut(s)	714, 870
BfrI	CTTAAG	1 cut(s)	1313
BfuAI	ACCTGC	1 cut(s)	427
BglI	GCCNNNNNGGC	1 cut(s)	1117
BglII	AGATCT	1 cut(s)	337
BisI	GCNGC	8 cut(s)	86, 129, 434, 591, 721, 1455, 1562, 1579
BlsI	GCNGC	8 cut(s)	87, 130, 435, 592, 722, 1456, 1563, 1580
Bme1390I	CCNGG	2 cut(s)	40, 705
Bme18I	GGWCC	1 cut(s)	1366
BmgT120I	GGNCC	4 cut(s)	57, 678, 679, 1366
BmiI	GGNNCC	8 cut(s)	44, 175, 589, 680, 681, 1217, 1378, 1614
BmrFI	CCNGG	2 cut(s)	40, 705
BmsI	GCATC	5 cut(s)	299, 356, 412, 658, 1442
BpiI	GAAGAC	3 cut(s)	255, 516, 1602
BpmI	CTGGAG	3 cut(s)	188, 1458, 1532
Bpu10I	CCTNAGC	1 cut(s)	936
Bpu14I	TTCGAA	2 cut(s)	1064, 1343
BpuEI	CTTGAG	1 cut(s)	1220
Bsa29I	ATCGAT	1 cut(s)	1536
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	2 cut(s)	296, 705
BsaJI	CCNNGG	4 cut(s)	38, 118, 704, 1372
BsaXI	ACNNNNNCTCC	2 cut(s)	741, 771
Bsc4I	CCNNNNNNNGG	3 cut(s)	66, 618, 1591
Bse118I	RCCGGY	1 cut(s)	66
Bse21I	CCTNAGG	1 cut(s)	613
Bse3DI	GCAATG	1 cut(s)	1106
BseBI	CCWGG	2 cut(s)	40, 705
BseCI	ATCGAT	1 cut(s)	1536
BseDI	CCNNGG	4 cut(s)	38, 118, 704, 1372
BseGI	GGATG	4 cut(s)	248, 274, 347, 502
BseLI	CCNNNNNNNGG	3 cut(s)	66, 618, 1591
BseMI	GCAATG	1 cut(s)	1106
BseMII	CTCAG	8 cut(s)	165, 174, 372, 456, 627, 675, 927, 1218
BseRI	GAGGAG	4 cut(s)	20, 203, 558, 1092
BseSI	GKGCMC	2 cut(s)	612, 682
BseXI	GCAGC	5 cut(s)	97, 140, 445, 732, 1548
BsgI	GTGCAG	1 cut(s)	104
Bsh1236I	CGCG	2 cut(s)	316, 1578
Bsh1285I	CGRYCG	1 cut(s)	71
BshFI	GGCC	5 cut(s)	58, 565, 622, 680, 800
BshNI	GGYRCC	2 cut(s)	42, 1376
BshVI	ATCGAT	1 cut(s)	1536
BsiEI	CGRYCG	1 cut(s)	71
BsiHKAI	GWGCWC	1 cut(s)	1518
BsiSI	CCGG	1 cut(s)	67
BslFI	GGGAC	1 cut(s)	1351
BslI	CCNNNNNNNGG	3 cut(s)	66, 618, 1591
BsmAI	GTCTC	6 cut(s)	12, 296, 455, 705, 1434, 1667
BsmBI	CGTCTC	2 cut(s)	12, 1434
BsmFI	GGGAC	1 cut(s)	1351
BsnI	GGCC	5 cut(s)	58, 565, 622, 680, 800
Bso31I	GGTCTC	2 cut(s)	296, 705
Bsp119I	TTCGAA	2 cut(s)	1064, 1343
Bsp120I	GGGCCC	1 cut(s)	678
Bsp1286I	GDGCHC	3 cut(s)	612, 682, 1518
Bsp143I	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
BspACI	CCGC	6 cut(s)	314, 351, 591, 1454, 1542, 1578
BspANI	GGCC	5 cut(s)	58, 565, 622, 680, 800
BspCNI	CTCAG	8 cut(s)	164, 173, 373, 457, 626, 676, 928, 1219
BspDI	ATCGAT	1 cut(s)	1536
BspFNI	CGCG	2 cut(s)	316, 1578
BspHI	TCATGA	1 cut(s)	1461
BspLI	GGNNCC	8 cut(s)	44, 175, 589, 680, 681, 1217, 1378, 1614
BspMAI	CTGCAG	2 cut(s)	718, 874
BspMI	ACCTGC	1 cut(s)	427
BspPI	GGATC	4 cut(s)	718, 1210, 1223, 1391
BspT104I	TTCGAA	2 cut(s)	1064, 1343
BspT107I	GGYRCC	2 cut(s)	42, 1376
BspTI	CTTAAG	1 cut(s)	1313
BspTNI	GGTCTC	2 cut(s)	296, 705
BsrDI	GCAATG	1 cut(s)	1106
BsrFI	RCCGGY	1 cut(s)	66
BssAI	RCCGGY	1 cut(s)	66
BssECI	CCNNGG	4 cut(s)	38, 118, 704, 1372
BssMI	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	1372
Bst2UI	CCWGG	2 cut(s)	40, 705
Bst4CI	ACNGT	7 cut(s)	119, 280, 449, 930, 1477, 1628, 1678
Bst6I	CTCTTC	3 cut(s)	15, 294, 392
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	1313
BstAPI	GCANNNNNTGC	1 cut(s)	599
BstBI	TTCGAA	2 cut(s)	1064, 1343
BstC8I	GCNNGC	4 cut(s)	624, 718, 742, 1050
BstDSI	CCRYGG	1 cut(s)	118
BstENI	CCTNNNNNAGG	1 cut(s)	616
BstF5I	GGATG	4 cut(s)	248, 274, 347, 502
BstFNI	CGCG	2 cut(s)	316, 1578
BstKTI	GATC	8 cut(s)	340, 713, 787, 963, 1024, 1218, 1399, 1555
BstMAI	GTCTC	6 cut(s)	12, 296, 455, 705, 1434, 1667
BstMBI	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
BstMCI	CGRYCG	1 cut(s)	71
BstMWI	GCNNNNNNNGC	4 cut(s)	599, 677, 750, 1117
BstNI	CCWGG	2 cut(s)	40, 705
BstNSI	RCATGY	1 cut(s)	602
BstSCI	CCNGG	2 cut(s)	38, 703
BstSFI	CTRYAG	2 cut(s)	714, 870
BstSLI	GKGCMC	2 cut(s)	612, 682
BstUI	CGCG	2 cut(s)	316, 1578
BstV1I	GCAGC	5 cut(s)	97, 140, 445, 732, 1548
BstV2I	GAAGAC	3 cut(s)	255, 516, 1602
BstX2I	RGATCY	4 cut(s)	337, 710, 1215, 1396
BstXI	CCANNNNNNTGG	1 cut(s)	364
BstYI	RGATCY	4 cut(s)	337, 710, 1215, 1396
Bsu15I	ATCGAT	1 cut(s)	1536
Bsu36I	CCTNAGG	1 cut(s)	613
BsuRI	GGCC	5 cut(s)	58, 565, 622, 680, 800
BsuTUI	ATCGAT	1 cut(s)	1536
BtgI	CCRYGG	1 cut(s)	118
BtsCI	GGATG	4 cut(s)	248, 274, 347, 502
BtsI	GCAGTG	1 cut(s)	1043
BtsIMutI	CAGTG	1 cut(s)	1043
BveI	ACCTGC	1 cut(s)	427
Cac8I	GCNNGC	4 cut(s)	624, 718, 742, 1050
CaiI	CAGNNNCTG	2 cut(s)	1436, 1676
CciI	TCATGA	1 cut(s)	1461
Cfr10I	RCCGGY	1 cut(s)	66
Cfr13I	GGNCC	4 cut(s)	57, 678, 679, 1366
ClaI	ATCGAT	1 cut(s)	1536
CseI	GACGC	1 cut(s)	712
Csp6I	GTAC	1 cut(s)	1148
CviQI	GTAC	1 cut(s)	1148
DpnI	GATC	8 cut(s)	339, 712, 786, 962, 1023, 1217, 1398, 1554
DpnII	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
Eam1104I	CTCTTC	3 cut(s)	15, 294, 392
EarI	CTCTTC	3 cut(s)	15, 294, 392
Eco130I	CCWWGG	1 cut(s)	1372
Eco24I	GRGCYC	1 cut(s)	682
Eco31I	GGTCTC	2 cut(s)	296, 705
Eco47I	GGWCC	1 cut(s)	1366
Eco57I	CTGAAG	2 cut(s)	74, 998
Eco81I	CCTNAGG	1 cut(s)	613
EcoNI	CCTNNNNNAGG	1 cut(s)	616
EcoO109I	RGGNCCY	1 cut(s)	679
EcoRII	CCWGG	2 cut(s)	38, 703
EcoT14I	CCWWGG	1 cut(s)	1372
EcoT38I	GRGCYC	1 cut(s)	682
ErhI	CCWWGG	1 cut(s)	1372
Esp3I	CGTCTC	2 cut(s)	12, 1434
FaqI	GGGAC	1 cut(s)	1351
FauI	CCCGC	1 cut(s)	1535
FbaI	TGATCA	1 cut(s)	784
Fnu4HI	GCNGC	8 cut(s)	86, 129, 434, 591, 721, 1455, 1562, 1579
FokI	GGATG	4 cut(s)	235, 281, 334, 509
FriOI	GRGCYC	1 cut(s)	682
Fsp4HI	GCNGC	8 cut(s)	86, 129, 434, 591, 721, 1455, 1562, 1579
FspBI	CTAG	4 cut(s)	216, 294, 341, 515
GluI	GCNGC	8 cut(s)	86, 129, 434, 591, 721, 1455, 1562, 1579
GsuI	CTGGAG	3 cut(s)	188, 1458, 1532
HaeIII	GGCC	5 cut(s)	58, 565, 622, 680, 800
HapII	CCGG	1 cut(s)	67
HgaI	GACGC	1 cut(s)	712
Hin1I	GRCGYC	1 cut(s)	5
HincII	GTYRAC	2 cut(s)	1038, 1473
HindII	GTYRAC	2 cut(s)	1038, 1473
HindIII	AAGCTT	2 cut(s)	742, 773
HinfI	GANTC	5 cut(s)	220, 260, 941, 1162, 1619
HpaII	CCGG	1 cut(s)	67
HphI	GGTGA	4 cut(s)	403, 461, 1269, 1558
Hpy166II	GTNNAC	5 cut(s)	122, 452, 1038, 1099, 1473
Hpy188I	TCNGA	8 cut(s)	154, 253, 268, 958, 1228, 1423, 1590, 1624
Hpy188III	TCNNGA	6 cut(s)	167, 1057, 1437, 1462, 1511, 1616
Hpy8I	GTNNAC	5 cut(s)	122, 452, 1038, 1099, 1473
Hpy99I	CGWCG	2 cut(s)	728, 971
HpyAV	CCTTC	5 cut(s)	382, 487, 973, 1112, 1585
HpyCH4III	ACNGT	7 cut(s)	119, 280, 449, 930, 1477, 1628, 1678
HpyCH4IV	ACGT	1 cut(s)	966
HpyF10VI	GCNNNNNNNGC	4 cut(s)	599, 677, 750, 1117
HpySE526I	ACGT	1 cut(s)	966
Hsp92I	GRCGYC	1 cut(s)	5
Ksp22I	TGATCA	1 cut(s)	784
Kzo9I	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
LmnI	GCTCC	2 cut(s)	587, 1513
Lsp1109I	GCAGC	5 cut(s)	97, 140, 445, 732, 1548
LweI	GCATC	5 cut(s)	299, 356, 412, 658, 1442
MaeI	CTAG	4 cut(s)	216, 294, 341, 515
MaeII	ACGT	1 cut(s)	966
MaeIII	GTNAC	3 cut(s)	443, 1183, 1327
MalI	GATC	8 cut(s)	339, 712, 786, 962, 1023, 1217, 1398, 1554
MboI	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
MfeI	CAATTG	2 cut(s)	426, 729
MflI	RGATCY	4 cut(s)	337, 710, 1215, 1396
MhlI	GDGCHC	3 cut(s)	612, 682, 1518
MluCI	AATT	8 cut(s)	426, 518, 525, 689, 729, 911, 1009, 1232
MlyI	GAGTC	2 cut(s)	214, 269
MroXI	GAANNNNTTC	1 cut(s)	1611
MseI	TTAA	3 cut(s)	947, 1314, 1383
MslI	CAYNNNNRTG	3 cut(s)	362, 1374, 1466
MspCI	CTTAAG	1 cut(s)	1313
MspI	CCGG	1 cut(s)	67
MspR9I	CCNGG	2 cut(s)	40, 705
MunI	CAATTG	2 cut(s)	426, 729
MvaI	CCWGG	2 cut(s)	40, 705
MvnI	CGCG	2 cut(s)	316, 1578
MwoI	GCNNNNNNNGC	4 cut(s)	599, 677, 750, 1117
NdeII	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
NlaIV	GGNNCC	8 cut(s)	44, 175, 589, 680, 681, 1217, 1378, 1614
NmuCI	GTSAC	2 cut(s)	443, 1327
NspI	RCATGY	1 cut(s)	602
NspV	TTCGAA	2 cut(s)	1064, 1343
OliI	CACNNNNGTG	2 cut(s)	362, 1374
PagI	TCATGA	1 cut(s)	1461
PdmI	GAANNNNTTC	1 cut(s)	1611
PfeI	GAWTC	3 cut(s)	941, 1162, 1619
PkrI	GCNGC	8 cut(s)	87, 130, 435, 592, 722, 1456, 1563, 1580
PleI	GAGTC	2 cut(s)	214, 268
PpsI	GAGTC	2 cut(s)	214, 268
Psp6I	CCWGG	2 cut(s)	38, 703
PspGI	CCWGG	2 cut(s)	38, 703
PspN4I	GGNNCC	8 cut(s)	44, 175, 589, 680, 681, 1217, 1378, 1614
PspOMI	GGGCCC	1 cut(s)	678
PspPI	GGNCC	4 cut(s)	57, 678, 679, 1366
PstI	CTGCAG	2 cut(s)	718, 874
PstNI	CAGNNNCTG	2 cut(s)	1436, 1676
PsuI	RGATCY	4 cut(s)	337, 710, 1215, 1396
RsaI	GTAC	1 cut(s)	1149
RsaNI	GTAC	1 cut(s)	1148
RseI	CAYNNNNRTG	3 cut(s)	362, 1374, 1466
SaqAI	TTAA	3 cut(s)	947, 1314, 1383
SatI	GCNGC	8 cut(s)	86, 129, 434, 591, 721, 1455, 1562, 1579
Sau3AI	GATC	8 cut(s)	337, 710, 784, 960, 1021, 1215, 1396, 1552
Sau96I	GGNCC	4 cut(s)	57, 678, 679, 1366
SchI	GAGTC	2 cut(s)	214, 269
ScrFI	CCNGG	2 cut(s)	40, 705
SduI	GDGCHC	3 cut(s)	612, 682, 1518
SfaNI	GCATC	5 cut(s)	299, 356, 412, 658, 1442
SfcI	CTRYAG	2 cut(s)	714, 870
SfuI	TTCGAA	2 cut(s)	1064, 1343
SinI	GGWCC	1 cut(s)	1366
SmiMI	CAYNNNNRTG	3 cut(s)	362, 1374, 1466
SmlI	CTYRAG	2 cut(s)	1199, 1313
SmoI	CTYRAG	2 cut(s)	1199, 1313
Sse9I	AATT	8 cut(s)	426, 518, 525, 689, 729, 911, 1009, 1232
SsiI	CCGC	6 cut(s)	314, 351, 591, 1454, 1542, 1578
SspMI	CTAG	4 cut(s)	216, 294, 341, 515
StyD4I	CCNGG	2 cut(s)	38, 703
StyI	CCWWGG	1 cut(s)	1372
TaaI	ACNGT	7 cut(s)	119, 280, 449, 930, 1477, 1628, 1678
TaiI	ACGT	1 cut(s)	969
TaqI	TCGA	6 cut(s)	71, 969, 1024, 1064, 1343, 1536
TasI	AATT	8 cut(s)	426, 518, 525, 689, 729, 911, 1009, 1232
TauI	GCSGC	3 cut(s)	593, 1457, 1581
TfiI	GAWTC	3 cut(s)	941, 1162, 1619
Tru1I	TTAA	3 cut(s)	947, 1314, 1383
Tru9I	TTAA	3 cut(s)	947, 1314, 1383
TscAI	CASTG	1 cut(s)	1050
TseFI	GTSAC	2 cut(s)	443, 1327
TseI	GCWGC	5 cut(s)	85, 128, 433, 720, 1561
Tsp45I	GTSAC	2 cut(s)	443, 1327
TspDTI	ATGAA	8 cut(s)	506, 660, 669, 754, 830, 899, 923, 1259
TspGWI	ACGGA	1 cut(s)	166
TspRI	CASTG	1 cut(s)	1050
Vha464I	CTTAAG	1 cut(s)	1313
VpaK11BI	GGWCC	1 cut(s)	1366
XagI	CCTNNNNNAGG	1 cut(s)	616
XapI	RAATTY	3 cut(s)	689, 1009, 1232
XceI	RCATGY	1 cut(s)	602
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1113
XmnI	GAANNNNTTC	1 cut(s)	1611
XspI	CTAG	4 cut(s)	216, 294, 341, 515

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.