pycom13g05750

CLAVATA3 ESR (CLE)-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0018288
Physical Location & Seq
Forward (+)
3792248 .. 3793511
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
ATGTTAGCCGGATATTCGTTTCAGGTAGCTCACCTACAATGCACATCCGGCAGTGGTTCCGGGCACTACCTGACAGGAGCCATGGATAAGGTACGCTTGCATCCCTCCAGTTGCTTGGGTCACAAGGCAGAGTGGGTGATTTTCAATGACTGTTGCGTTGTGGAAACCAAGAAGTTTATCCTCACAGTGACCCAGATTCCCAGCGAAGGGCTCGTTGCTGGTAGCTCTCACTCTTCTTTTTCGTTTCCCTCCTCCACTTCCGGCCTCCCGCGGCTTCTTCTCATGGCGGCAGCGGCGGTCATCGTCTCCCTACTTTTTGTCACATCCGCTGCCACACCCATCTCTAACCCAACCGATGCTACACCAAGAAACCCCCACCGCCTCCATAGTCAAGAAACCCACCACCACGGTGAATGCGATCATCAGACTTCAGCTTCGGAATCGTCGAGCGCACAATTACGTTCTTTGTGCCTTCAACTCACCCACCACAGAATATTCCACGCCAGCCTTCCCCTCTCACCGCTGTCACCGGTATCCCCATCCGGCCCCTCGCAGCCGGCTCCTGGCGACCGTCAAGATGGTCACGAGATTGATCCAAGATACGGTGTGGAGAAGAGATTAGTCCCCTCCGGTCCCAACCCTCTTCACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

23.16

Weight (kDa)

6.96

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OB_NTP_bind PF07717 1 - 73 1e-08 Oligonucleotide/oligosaccharide-binding (OB)-fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26600 AT1G26600 AT1G69320
fragaria_vesca FvH4_4g30410
prunus_persica Prupe.1G283100_v2.0.a1
pyrus_communis pycom13g05750
rosa_chinensis RchiOBHm_Chr4g0438701
rosa_laevigata RLG00000006336
rosa_rugosa Rorug04G0312700
rosa_wichuraiana Rw0G016720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 271
AciI CCGC 8 cut(s) 269, 271, 287, 293, 296, 327, 379, 521
AclWI GGATC 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 414
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 4 cut(s) 206, 207, 563, 602
AgeI ACCGGT 1 cut(s) 529
AgsI TTSAA 2 cut(s) 145, 476
AjnI CCWGG 1 cut(s) 562
AleI CACNNNNGTG 1 cut(s) 408
AluBI AGCT 3 cut(s) 29, 225, 434
AluI AGCT 3 cut(s) 29, 225, 434
Alw26I GTCTC 1 cut(s) 310
AlwI GGATC 1 cut(s) 587
AoxI GGCC 2 cut(s) 262, 544
ApeKI GCWGC 3 cut(s) 290, 329, 553
ArsI GACNNNNNNTTYG 2 cut(s) 418, 450
AsiGI ACCGGT 1 cut(s) 529
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 2 cut(s) 545, 632
AsuC2I CCSGG 1 cut(s) 61
AsuHPI GGTGA 6 cut(s) 23, 148, 422, 472, 510, 519
AvaII GGWCC 1 cut(s) 632
BaeGI GKGCMC 1 cut(s) 66
BanII GRGCYC 1 cut(s) 213
BauI CACGAG 1 cut(s) 584
BbvI GCAGC 3 cut(s) 302, 316, 565
BccI CCATC 3 cut(s) 347, 547, 572
BciT130I CCWGG 1 cut(s) 564
BciVI GTATCC 1 cut(s) 544
BcnI CCSGG 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 310
BfuI GTATCC 1 cut(s) 544
BisI GCNGC 6 cut(s) 272, 288, 291, 294, 330, 554
BlsI GCNGC 6 cut(s) 273, 289, 292, 295, 331, 555
Bme1390I CCNGG 2 cut(s) 61, 564
Bme18I GGWCC 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 545, 632
BmiI GGNNCC 5 cut(s) 58, 79, 547, 561, 634
BmrFI CCNGG 2 cut(s) 61, 564
BmsI GCATC 2 cut(s) 109, 346
BpmI CTGGAG 1 cut(s) 91
BpuMI CCSGG 1 cut(s) 61
BsaJI CCNNGG 3 cut(s) 81, 269, 406
BsaWI WCCGGW 2 cut(s) 529, 629
Bsc4I CCNNNNNNNGG 4 cut(s) 206, 207, 563, 602
Bse118I RCCGGY 2 cut(s) 529, 556
Bse1I ACTGG 1 cut(s) 108
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 81, 269, 406
BseGI GGATG 4 cut(s) 44, 100, 323, 539
BseLI CCNNNNNNNGG 4 cut(s) 206, 207, 563, 602
BseNI ACTGG 1 cut(s) 108
BseRI GAGGAG 1 cut(s) 241
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 3 cut(s) 302, 316, 565
BseYI CCCAGC 1 cut(s) 200
Bsh1236I CGCG 1 cut(s) 271
Bsh1285I CGRYCG 1 cut(s) 571
BshFI GGCC 2 cut(s) 264, 546
BshTI ACCGGT 1 cut(s) 529
BsiEI CGRYCG 1 cut(s) 571
BsiSI CCGG 8 cut(s) 9, 48, 60, 261, 530, 543, 557, 630
BslFI GGGAC 2 cut(s) 608, 618
BslI CCNNNNNNNGG 4 cut(s) 206, 207, 563, 602
BsmAI GTCTC 1 cut(s) 310
BsmBI CGTCTC 1 cut(s) 310
BsmFI GGGAC 2 cut(s) 608, 618
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 2 cut(s) 264, 546
Bsp1286I GDGCHC 2 cut(s) 66, 213
Bsp143I GATC 2 cut(s) 418, 592
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 8 cut(s) 269, 271, 287, 293, 296, 327, 379, 521
BspANI GGCC 2 cut(s) 264, 546
BspFNI CGCG 1 cut(s) 271
BspLI GGNNCC 5 cut(s) 58, 79, 547, 561, 634
BspPI GGATC 1 cut(s) 587
BsrFI RCCGGY 2 cut(s) 529, 556
BsrI ACTGG 1 cut(s) 108
BssAI RCCGGY 2 cut(s) 529, 556
BssECI CCNNGG 3 cut(s) 81, 269, 406
BssMI GATC 2 cut(s) 418, 592
BssSI CACGAG 1 cut(s) 584
BssT1I CCWWGG 1 cut(s) 81
Bst2BI CACGAG 1 cut(s) 584
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 5 cut(s) 152, 187, 410, 572, 605
Bst6I CTCTTC 3 cut(s) 238, 608, 648
BstC8I GCNNGC 3 cut(s) 98, 505, 558
BstDSI CCRYGG 3 cut(s) 81, 269, 406
BstF5I GGATG 4 cut(s) 44, 100, 323, 539
BstFNI CGCG 1 cut(s) 271
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 2 cut(s) 421, 595
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 2 cut(s) 418, 592
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 2 cut(s) 48, 293
BstNI CCWGG 1 cut(s) 564
BstSCI CCNGG 2 cut(s) 59, 562
BstSLI GKGCMC 1 cut(s) 66
BstUI CGCG 1 cut(s) 271
BstV1I GCAGC 3 cut(s) 302, 316, 565
BstXI CCANNNNNNTGG 1 cut(s) 115
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 2 cut(s) 264, 546
BtgI CCRYGG 3 cut(s) 81, 269, 406
BtsCI GGATG 4 cut(s) 44, 100, 323, 539
BtsI GCAGTG 1 cut(s) 58
BtsIMutI CAGTG 2 cut(s) 58, 192
Cac8I GCNNGC 3 cut(s) 98, 505, 558
CfoI GCGC 1 cut(s) 452
Cfr10I RCCGGY 2 cut(s) 529, 556
Cfr13I GGNCC 2 cut(s) 545, 632
Cfr42I CCGCGG 1 cut(s) 272
Csp6I GTAC 1 cut(s) 92
CspAI ACCGGT 1 cut(s) 529
CviAII CATG 2 cut(s) 82, 283
CviQI GTAC 1 cut(s) 92
DpnI GATC 2 cut(s) 420, 594
DpnII GATC 2 cut(s) 418, 592
Eam1104I CTCTTC 3 cut(s) 238, 608, 648
EarI CTCTTC 3 cut(s) 238, 608, 648
Eco130I CCWWGG 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 1 cut(s) 632
Eco57I CTGAAG 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 81
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 310
FaeI CATG 2 cut(s) 85, 286
FaiI YATR 3 cut(s) 83, 284, 387
FalI AAGNNNNNCTT 2 cut(s) 80, 112
FaqI GGGAC 2 cut(s) 608, 618
FatI CATG 2 cut(s) 81, 282
FauI CCCGC 1 cut(s) 276
Fnu4HI GCNGC 6 cut(s) 272, 288, 291, 294, 330, 554
FokI GGATG 4 cut(s) 31, 87, 310, 526
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 6 cut(s) 272, 288, 291, 294, 330, 554
GlaI GCGC 1 cut(s) 451
GluI GCNGC 6 cut(s) 272, 288, 291, 294, 330, 554
GsaI CCCAGC 1 cut(s) 204
GsuI CTGGAG 1 cut(s) 91
HaeIII GGCC 2 cut(s) 264, 546
HapII CCGG 8 cut(s) 9, 48, 60, 261, 530, 543, 557, 630
HhaI GCGC 1 cut(s) 452
Hin1II CATG 2 cut(s) 85, 286
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 2 cut(s) 196, 440
HpaII CCGG 8 cut(s) 9, 48, 60, 261, 530, 543, 557, 630
HphI GGTGA 6 cut(s) 23, 148, 422, 472, 510, 519
Hpy188I TCNGA 2 cut(s) 426, 439
Hpy188III TCNNGA 3 cut(s) 392, 575, 584
Hpy99I CGWCG 1 cut(s) 448
HpyAV CCTTC 3 cut(s) 200, 482, 518
HpyCH4III ACNGT 5 cut(s) 152, 187, 410, 572, 605
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 2 cut(s) 42, 100
HpyF10VI GCNNNNNNNGC 2 cut(s) 48, 293
HpySE526I ACGT 1 cut(s) 460
Hsp92II CATG 2 cut(s) 85, 286
HspAI GCGC 1 cut(s) 450
KroI GCCGGC 1 cut(s) 556
KroNI GCCGGC 1 cut(s) 558
KspI CCGCGG 1 cut(s) 272
Kzo9I GATC 2 cut(s) 418, 592
LmnI GCTCC 2 cut(s) 77, 565
Lsp1109I GCAGC 3 cut(s) 302, 316, 565
LweI GCATC 2 cut(s) 109, 346
MaeII ACGT 1 cut(s) 460
MaeIII GTNAC 5 cut(s) 119, 187, 319, 525, 581
MalI GATC 2 cut(s) 420, 594
MboI GATC 2 cut(s) 418, 592
MboII GAAGA 4 cut(s) 225, 269, 625, 635
MfeI CAATTG 1 cut(s) 649
MhlI GDGCHC 2 cut(s) 66, 213
MluCI AATT 2 cut(s) 455, 649
MroNI GCCGGC 1 cut(s) 556
MslI CAYNNNNRTG 1 cut(s) 408
MspA1I CMGCKG 4 cut(s) 271, 293, 329, 523
MspI CCGG 8 cut(s) 9, 48, 60, 261, 530, 543, 557, 630
MspR9I CCNGG 2 cut(s) 61, 564
MunI CAATTG 1 cut(s) 649
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 2 cut(s) 48, 293
NaeI GCCGGC 1 cut(s) 558
NciI CCSGG 1 cut(s) 61
NcoI CCATGG 1 cut(s) 81
NdeII GATC 2 cut(s) 418, 592
NgoMIV GCCGGC 1 cut(s) 556
NlaIII CATG 2 cut(s) 85, 286
NlaIV GGNNCC 5 cut(s) 58, 79, 547, 561, 634
NmuCI GTSAC 5 cut(s) 119, 187, 319, 525, 581
OliI CACNNNNGTG 1 cut(s) 408
PcsI WCGNNNNNNNCGW 2 cut(s) 414, 443
PctI GAATGC 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 196, 440
PinAI ACCGGT 1 cut(s) 529
PkrI GCNGC 6 cut(s) 273, 289, 292, 295, 331, 555
Psp6I CCWGG 1 cut(s) 562
PspFI CCCAGC 1 cut(s) 200
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 5 cut(s) 58, 79, 547, 561, 634
PspPI GGNCC 2 cut(s) 545, 632
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 408
SacII CCGCGG 1 cut(s) 272
SatI GCNGC 6 cut(s) 272, 288, 291, 294, 330, 554
Sau3AI GATC 2 cut(s) 418, 592
Sau96I GGNCC 2 cut(s) 545, 632
ScrFI CCNGG 2 cut(s) 61, 564
SduI GDGCHC 2 cut(s) 66, 213
SetI ASST 8 cut(s) 27, 31, 36, 72, 93, 227, 436, 463
SfaNI GCATC 2 cut(s) 109, 346
Sfr303I CCGCGG 1 cut(s) 272
SgrBI CCGCGG 1 cut(s) 272
SinI GGWCC 1 cut(s) 632
SmiMI CAYNNNNRTG 1 cut(s) 408
Sse9I AATT 2 cut(s) 455, 649
SsiI CCGC 8 cut(s) 269, 271, 287, 293, 296, 327, 379, 521
SspI AATATT 1 cut(s) 495
StyD4I CCNGG 2 cut(s) 59, 562
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 5 cut(s) 152, 187, 410, 572, 605
TaiI ACGT 1 cut(s) 463
TaqI TCGA 1 cut(s) 446
TasI AATT 2 cut(s) 455, 649
TauI GCSGC 3 cut(s) 274, 290, 296
TfiI GAWTC 2 cut(s) 196, 440
TscAI CASTG 2 cut(s) 58, 192
TseFI GTSAC 5 cut(s) 119, 187, 319, 525, 581
TseI GCWGC 3 cut(s) 290, 329, 553
Tsp45I GTSAC 5 cut(s) 119, 187, 319, 525, 581
TspRI CASTG 2 cut(s) 58, 192
VpaK11BI GGWCC 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.