pycom13g08180

Belongs to the PI3 PI4-kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010181
Physical Location & Seq
Reverse (-)
5432827 .. 5438843
6017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2376 bp
ATGAGCGGGAACGAGTTCCGCTTCTTCCTGTCCTGCGACATCAACCTCCCGGTAACCTTCCGGATCGAACGGCTAGAAGGATCCCTGCCTCCCCCCAAGTCACCTAGCTCCGACGTTGACCCCACTTCCGCGACAGAAGAGAGAAGACCAGAGCTGTACGTGGAGTGCGCATTGTACATTGATGGTGCTCCGTTTGGGCTTCCGACTAGGACAAGGTTGGAATCTTCAGGGCCATTATATTGTTGGAACGAACTCATTACTCTGAGTACGAAATACCGAGACTTAACCGGTCACTCACAACTTGCTTTGACAGTTTGGGATGTTTCATGTGGGAAGGATGAAGGGTTGGTTGGTGGTGCTACAATTCTTCTTTTTAATAGCAAAAAGCAACTCAAAACTGGGGAGCAAAAGCTGAGGCTTTGGAAGGGAAAAGTAGCAGATCGATCGTTTCCTACTTCTACTCCTGGAAAGGTCCCCAGGCATGAGCGTGGGGAGTTGGAACGGTTGGAGAAGCTAATGAATAGGTATGAGAGGGGGCAGATCCAATGTGTTGATTGGCTGGACCGCCTGGCTTTTAAAGCTATGGAGAGAATAAAGGAACGTGAAAGCTCTAAAAATGGAAGTTTGCATTTATACCTGGTTGTCGATTTCTGTAGTTTCGAACATCGTGTTGTTTTCCAGGAATCAGGGACAAATTTCTTATTACCATCCCCAATAGCTTCATCAAATGAAATTGTCACGGTCTGGGACCCGGAAGTGGGAAAGATAAATCCATCAGAGAACAAGCAACTAAAACTTGCTAGGAGTTTAACACGTGGTATCATTGACAGGGATCTCAAACCAAGCTCCAACGAAAGAAAGTCGATACATTGGATTTTGAAATACCCACCAACAAGGACTTTGAGTGGCGATGAGAGGCAGCTCCTCTGGAAATTTCGTTTCTCATTGATGTCAGAGAAGAGGGCCCTCACAAAGTTCCTCAGATGTGTTGAATGGAGTGATGTTCAGGAAGCAAAACAAGCGTTAGAACTGATGGGCAAATGGAGCATGATTGATGTCTGCGACGCATTAGAGCTGCTATCTCCTGTTTTTGAAAGTGAAGAGGTTCGTGCATATGCTGTCAGTGTTCTTGAAAGAGCTGACGATGAAGAGCTCCAGTGTTACTTACTTCAACTGGTTCAGGCTCTTCGATTTGAGCGCTCTGATAAATCTCGCCTTTCTCATTTCCTTGTGCAACGTGAACTTCATGATCCAGCATATGCCAAACGGTTTTACTGTACCTACGAAATTCTGGAAGAGAATATGATGAAGTTGCCAGCTGGTGTGCAAGGAGATGAAGATGGATTTAAACTGTGGCAAAGTTTGGTGCGCCAGACAGAATTGACTGCACAGTTGTGTTCAATAATGAGAGATGTAAGAAACGTACGCGGCAATACCCAGAAGAAGATTGAAAAGCTTAGACAACTACTTTCTGGTCTTCTTAGTGAACTTACGTATTTTGAAGAGCCAATAAGATCACCACTGGCTCCAGGAGTCCTCATCACTGGGATCGTGCCATCTGAGTCATCAATATTCAAAAGTGCACTGCATCCTTTGCGTTTGACTTTCCGAGCAGCAAATGGGGGAAGTTGCAAAATTATCTTTAAGAAGGGGGATGATATCCGCCAAGACCAATTAGTTGTTCAAATGGTATATCTCATGGATCGATTGCTGAAATTGGAAAATCTCGATCTGCACTTAACTCCTTATAGAGTTCTTGCGACTGGGCAAGATGAAGGAATGCTAGAGTTCATACCATCCCGCTCATTAGCACAGATTCTCTCAGAGCATAGGAGCATTACAAGCTACCTGCAGAAATTTCATCCAGATGAGCATGGACCGTTTGGTATTACTGCTACCTGTCTGGAAACATTTATAAAAAGCTGTGCTGGCTATTCTGTTATCACATATATACTGGGCATTGGAGACAGGCATCTTGATAACCTTCTCCTTGTGGACGATGGACGTCTTTTCCATGTTGATTTTGGCTTCATTCTGGGTCGTGATCCAAAGCCATTTCCACCGCCAATGAAGCTTTGCAAAGAAATGGTTGAGGCTATGGGTGGAGCTGAAAGCCAGTACTATACAAGGTTCAAATCCTACTGTTGCGAAGCATACAACATCATTAGGAAATCCAGTAACCTAATCTTGAACCTGTTTCACCTAATGGCGGGTTCCAACATTCCTGACATAGCTTCGGATCCTGAAAAAGGCATCCTCAAGCTCCAGGAGAAGTTCCGGTTGGACTTGGACGATGAAGCTTGCATACATTTCTTCCAGGACTTGATCAATGAGAGTTTGGGTGTCATCTACATCTTTGAAAGTTACCGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000407 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005777 GO:0005942 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006897 GO:0006914 GO:0006950 GO:0006970 GO:0006996 GO:0007033 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009056 GO:0009058 GO:0009555 GO:0009628 GO:0009651 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016236 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019637 GO:0019898 GO:0022607 GO:0023052 GO:0030242 GO:0030258 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032991 GO:0034271 GO:0034272 GO:0035004 GO:0035032 GO:0035556 GO:0036092 GO:0036211 GO:0042579 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045017 GO:0046474 GO:0046486 GO:0046488 GO:0046834 GO:0046854 GO:0046907 GO:0048015 GO:0048017 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0052742 GO:0055046 GO:0061695 GO:0061919 GO:0065007 GO:0070925 GO:0071704 GO:0071840 GO:0072593 GO:0090407 GO:0097708 GO:0098657 GO:0098796 GO:1901564 GO:1901576 GO:1902494 GO:1905037 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04070 ko04136 ko04138 ko04140 ko04145 ko04371 ko05152 ko05167 map00562 map01100 map04070 map04136 map04138 map04140 map04145 map04371 map05152 map05167
Pfam Domains
Protein Families
PI3K_C2 PI3Ka PI3_PI4_kinase

Protein Analysis

792

Amino Acids

90.55

Weight (kDa)

6.31

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI3K_C2 PF00792 50 - 192 2.1e-41 Phosphoinositide 3-kinase C2
PI3Ka PF00613 278 - 425 2.4e-53 Phosphoinositide 3-kinase family, accessory domain (PIK domain)
PI3_PI4_kinase PF00454 544 - 745 9.5e-57 Phosphatidylinositol 3- and 4-kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1916
AatII GACGTC 1 cut(s) 2008
Acc16I TGCGCA 1 cut(s) 169
Acc36I ACCTGC 1 cut(s) 1857
AccBSI CCGCTC 2 cut(s) 6, 1803
AccII CGCG 2 cut(s) 131, 1428
AccIII TCCGGA 1 cut(s) 60
AciI CCGC 9 cut(s) 6, 19, 129, 565, 1428, 1663, 1801, 2063, 2210
AcsI RAATTY 4 cut(s) 694, 932, 1287, 1856
AcuI CTGAAG 1 cut(s) 210
AcvI CACGTG 1 cut(s) 815
AcyI GRCGYC 1 cut(s) 2005
AfaI GTAC 6 cut(s) 158, 176, 268, 1279, 1425, 2120
AfeI AGCGCT 1 cut(s) 1199
AfiI CCNNNNNNNGG 4 cut(s) 757, 758, 2209, 2249
AflIII ACRYGT 1 cut(s) 812
AgeI ACCGGT 2 cut(s) 287, 2367
AjnI CCWGG 8 cut(s) 463, 476, 567, 636, 678, 1528, 2265, 2316
AjuI GAANNNNNNNTTGG 6 cut(s) 333, 365, 1665, 1697, 2264, 2296
AleI CACNNNNGTG 1 cut(s) 1393
Alw21I GWGCWC 3 cut(s) 190, 1155, 1585
Alw26I GTCTC 2 cut(s) 273, 1959
Alw44I GTGCAC 1 cut(s) 1581
Aor13HI TCCGGA 1 cut(s) 60
Aor51HI AGCGCT 1 cut(s) 1199
AoxI GGCC 2 cut(s) 230, 963
ApaI GGGCCC 1 cut(s) 967
ApaLI GTGCAC 1 cut(s) 1581
ApeKI GCWGC 3 cut(s) 919, 1075, 1613
ApoI RAATTY 4 cut(s) 694, 932, 1287, 1856
AsiGI ACCGGT 2 cut(s) 287, 2367
Asp700I GAANNNNTTC 2 cut(s) 14, 1104
AspLEI GCGC 3 cut(s) 170, 1200, 1371
AspS9I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1877
AsuC2I CCSGG 2 cut(s) 50, 752
AsuHPI GGTGA 3 cut(s) 93, 1509, 2192
AsuII TTCGAA 1 cut(s) 660
AvaII GGWCC 4 cut(s) 472, 562, 748, 1877
BaeGI GKGCMC 2 cut(s) 967, 1585
BamHI GGATCC 2 cut(s) 80, 2239
BanII GRGCYC 2 cut(s) 967, 1155
BarI GAAGNNNNNNTAC 2 cut(s) 2289, 2321
BbrPI CACGTG 1 cut(s) 815
BbsI GAAGAC 2 cut(s) 151, 1469
Bbv12I GWGCWC 3 cut(s) 190, 1155, 1585
BbvCI CCTCAGC 1 cut(s) 413
BbvI GCAGC 3 cut(s) 931, 1062, 1625
BccI CCATC 8 cut(s) 176, 715, 781, 1027, 1334, 1564, 1804, 1994
BceAI ACGGC 1 cut(s) 86
BcgI CGANNNNNNTGC 2 cut(s) 426, 460
BciT130I CCWGG 8 cut(s) 465, 478, 569, 638, 680, 1530, 2267, 2318
BclI TGATCA 1 cut(s) 2325
BcnI CCSGG 2 cut(s) 50, 752
BcoDI GTCTC 2 cut(s) 273, 1959
BfaI CTAG 5 cut(s) 74, 105, 207, 801, 1784
BfmI CTRYAG 2 cut(s) 652, 1850
BfoI RGCGCY 1 cut(s) 1201
BfuAI ACCTGC 1 cut(s) 1857
BisI GCNGC 4 cut(s) 920, 1076, 1429, 1614
BlsI GCNGC 4 cut(s) 921, 1077, 1430, 1615
BmcAI AGTACT 1 cut(s) 2120
Bme18I GGWCC 4 cut(s) 472, 562, 748, 1877
BmgT120I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1877
BmiI GGNNCC 8 cut(s) 82, 474, 749, 750, 965, 1527, 2215, 2241
BmrI ACTGGG 4 cut(s) 408, 1554, 1773, 1964
BmsI GCATC 3 cut(s) 1597, 1981, 2262
BmuI ACTGGG 4 cut(s) 408, 1554, 1773, 1964
BpiI GAAGAC 2 cut(s) 151, 1469
BpmI CTGGAG 3 cut(s) 1139, 1512, 2249
Bpu10I CCTNAGC 1 cut(s) 413
Bpu14I TTCGAA 1 cut(s) 660
BpuEI CTTGAG 1 cut(s) 2243
BpuMI CCSGG 2 cut(s) 50, 752
Bsa29I ATCGAT 2 cut(s) 442, 1705
BsaAI YACGTR 3 cut(s) 160, 815, 1494
BsaHI GRCGYC 1 cut(s) 2005
BsaJI CCNNGG 1 cut(s) 476
BsaWI WCCGGW 4 cut(s) 60, 287, 2277, 2367
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 4 cut(s) 757, 758, 2209, 2249
Bse118I RCCGGY 2 cut(s) 287, 2367
Bse1I ACTGG 9 cut(s) 403, 1156, 1179, 1527, 1549, 1768, 1959, 2116, 2175
BseAI TCCGGA 1 cut(s) 60
BseBI CCWGG 8 cut(s) 465, 478, 569, 638, 680, 1530, 2267, 2318
BseCI ATCGAT 2 cut(s) 442, 1705
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 8 cut(s) 325, 343, 707, 1588, 1660, 1796, 1861, 2253
BseLI CCNNNNNNNGG 4 cut(s) 757, 758, 2209, 2249
BseMII CTCAG 5 cut(s) 254, 404, 994, 1551, 1836
BseNI ACTGG 9 cut(s) 403, 1156, 1179, 1527, 1549, 1768, 1959, 2116, 2175
BseRI GAGGAG 1 cut(s) 914
BseSI GKGCMC 2 cut(s) 967, 1585
BseXI GCAGC 3 cut(s) 931, 1062, 1625
BsgI GTGCAG 2 cut(s) 1371, 1718
Bsh1236I CGCG 2 cut(s) 131, 1428
Bsh1285I CGRYCG 1 cut(s) 446
BshFI GGCC 2 cut(s) 232, 965
BshTI ACCGGT 2 cut(s) 287, 2367
BshVI ATCGAT 2 cut(s) 442, 1705
BsiEI CGRYCG 1 cut(s) 446
BsiHKAI GWGCWC 3 cut(s) 190, 1155, 1585
BsiSI CCGG 6 cut(s) 50, 61, 288, 752, 2278, 2368
BsiWI CGTACG 1 cut(s) 1423
BslFI GGGAC 3 cut(s) 458, 703, 761
BslI CCNNNNNNNGG 4 cut(s) 757, 758, 2209, 2249
BsmAI GTCTC 2 cut(s) 273, 1959
BsmFI GGGAC 3 cut(s) 458, 703, 761
BsmI GAATGC 1 cut(s) 1785
BsnI GGCC 2 cut(s) 232, 965
Bsp119I TTCGAA 1 cut(s) 660
Bsp120I GGGCCC 1 cut(s) 963
Bsp1286I GDGCHC 4 cut(s) 190, 967, 1155, 1585
Bsp13I TCCGGA 1 cut(s) 60
Bsp1407I TGTACA 1 cut(s) 174
BspACI CCGC 9 cut(s) 6, 19, 129, 565, 1428, 1663, 1801, 2063, 2210
BspANI GGCC 2 cut(s) 232, 965
BspCNI CTCAG 5 cut(s) 255, 405, 993, 1552, 1835
BspDI ATCGAT 2 cut(s) 442, 1705
BspEI TCCGGA 1 cut(s) 60
BspFNI CGCG 2 cut(s) 131, 1428
BspHI TCATGA 1 cut(s) 1246
BspLI GGNNCC 8 cut(s) 82, 474, 749, 750, 965, 1527, 2215, 2241
BspMAI CTGCAG 1 cut(s) 1854
BspMI ACCTGC 1 cut(s) 1857
BspQI GCTCTTC 3 cut(s) 1143, 1191, 1497
BspT104I TTCGAA 1 cut(s) 660
BsrBI CCGCTC 2 cut(s) 6, 1803
BsrFI RCCGGY 2 cut(s) 287, 2367
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 9 cut(s) 403, 1156, 1179, 1527, 1549, 1768, 1959, 2116, 2175
BssAI RCCGGY 2 cut(s) 287, 2367
BssECI CCNNGG 1 cut(s) 476
BssNI GRCGYC 1 cut(s) 2005
Bst2UI CCWGG 8 cut(s) 465, 478, 569, 638, 680, 1530, 2267, 2318
Bst4CI ACNGT 9 cut(s) 313, 504, 742, 1269, 1277, 1353, 1392, 1881, 2144
Bst6I CTCTTC 7 cut(s) 132, 953, 1095, 1143, 1191, 1290, 1497
BstACI GRCGYC 1 cut(s) 2005
BstAPI GCANNNNNTGC 1 cut(s) 1594
BstAUI TGTACA 1 cut(s) 174
BstBAI YACGTR 3 cut(s) 160, 815, 1494
BstBI TTCGAA 1 cut(s) 660
BstC8I GCNNGC 3 cut(s) 1317, 1930, 2302
BstDEI CTNAG 7 cut(s) 263, 413, 980, 1457, 1481, 1560, 1822
BstEII GGTNACC 1 cut(s) 52
BstENI CCTNNNNNAGG 1 cut(s) 2247
BstF5I GGATG 8 cut(s) 325, 343, 707, 1588, 1660, 1796, 1861, 2253
BstFNI CGCG 2 cut(s) 131, 1428
BstH2I RGCGCY 1 cut(s) 1201
BstHHI GCGC 3 cut(s) 170, 1200, 1371
BstMAI GTCTC 2 cut(s) 273, 1959
BstMCI CGRYCG 1 cut(s) 446
BstMWI GCNNNNNNNGC 7 cut(s) 578, 1019, 1044, 1594, 1842, 1929, 2071
BstNI CCWGG 8 cut(s) 465, 478, 569, 638, 680, 1530, 2267, 2318
BstPI GGTNACC 1 cut(s) 52
BstSFI CTRYAG 2 cut(s) 652, 1850
BstSLI GKGCMC 2 cut(s) 967, 1585
BstSNI TACGTA 1 cut(s) 1494
BstUI CGCG 2 cut(s) 131, 1428
BstV1I GCAGC 3 cut(s) 931, 1062, 1625
BstV2I GAAGAC 2 cut(s) 151, 1469
BstX2I RGATCY 4 cut(s) 80, 540, 832, 2239
BstYI RGATCY 4 cut(s) 80, 540, 832, 2239
Bsu15I ATCGAT 2 cut(s) 442, 1705
BsuRI GGCC 2 cut(s) 232, 965
BsuTUI ATCGAT 2 cut(s) 442, 1705
BtgZI GCGATG 1 cut(s) 924
BtsCI GGATG 8 cut(s) 325, 343, 707, 1588, 1660, 1796, 1861, 2253
BtsI GCAGTG 1 cut(s) 1583
BtsIMutI CAGTG 5 cut(s) 1129, 1163, 1520, 1542, 1583
BveI ACCTGC 1 cut(s) 1857
Cac8I GCNNGC 3 cut(s) 1317, 1930, 2302
CciI TCATGA 1 cut(s) 1246
CfoI GCGC 3 cut(s) 170, 1200, 1371
Cfr10I RCCGGY 2 cut(s) 287, 2367
Cfr13I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1877
ClaI ATCGAT 2 cut(s) 442, 1705
CseI GACGC 1 cut(s) 1073
CsiI ACCWGGT 1 cut(s) 636
Csp6I GTAC 6 cut(s) 157, 175, 267, 1278, 1424, 2119
CspAI ACCGGT 2 cut(s) 287, 2367
CviAII CATG 7 cut(s) 327, 482, 1048, 1247, 1699, 1874, 2015
CviQI GTAC 6 cut(s) 157, 175, 267, 1278, 1424, 2119
DdeI CTNAG 7 cut(s) 263, 413, 980, 1457, 1481, 1560, 1822
DraI TTTAAA 2 cut(s) 577, 1348
Eam1104I CTCTTC 7 cut(s) 132, 953, 1095, 1143, 1191, 1290, 1497
EarI CTCTTC 7 cut(s) 132, 953, 1095, 1143, 1191, 1290, 1497
EciI GGCGGA 1 cut(s) 1652
Ecl136II GAGCTC 1 cut(s) 1153
Eco105I TACGTA 1 cut(s) 1494
Eco24I GRGCYC 2 cut(s) 967, 1155
Eco32I GATATC 1 cut(s) 1660
Eco47I GGWCC 4 cut(s) 472, 562, 748, 1877
Eco47III AGCGCT 1 cut(s) 1199
Eco53kI GAGCTC 1 cut(s) 1153
Eco57I CTGAAG 1 cut(s) 210
Eco72I CACGTG 1 cut(s) 815
Eco91I GGTNACC 1 cut(s) 52
EcoICRI GAGCTC 1 cut(s) 1153
EcoNI CCTNNNNNAGG 1 cut(s) 2247
EcoO109I RGGNCCY 4 cut(s) 472, 748, 963, 964
EcoO65I GGTNACC 1 cut(s) 52
EcoRII CCWGG 8 cut(s) 463, 476, 567, 636, 678, 1528, 2265, 2316
EcoRV GATATC 1 cut(s) 1660
EcoT38I GRGCYC 2 cut(s) 967, 1155
FaeI CATG 7 cut(s) 330, 485, 1051, 1250, 1702, 1877, 2018
FaqI GGGAC 3 cut(s) 458, 703, 761
FatI CATG 7 cut(s) 326, 481, 1047, 1246, 1698, 1873, 2014
FauI CCCGC 2 cut(s) 1808, 2203
FauNDI CATATG 2 cut(s) 1114, 1258
FbaI TGATCA 1 cut(s) 2325
Fnu4HI GCNGC 4 cut(s) 920, 1076, 1429, 1614
FokI GGATG 8 cut(s) 332, 350, 694, 1575, 1667, 1783, 1848, 2240
FriOI GRGCYC 2 cut(s) 967, 1155
Fsp4HI GCNGC 4 cut(s) 920, 1076, 1429, 1614
FspAI RTGCGCAY 1 cut(s) 169
FspBI CTAG 5 cut(s) 74, 105, 207, 801, 1784
FspI TGCGCA 1 cut(s) 169
GlaI GCGC 3 cut(s) 169, 1199, 1370
GluI GCNGC 4 cut(s) 920, 1076, 1429, 1614
GsuI CTGGAG 3 cut(s) 1139, 1512, 2249
HaeII RGCGCY 1 cut(s) 1201
HaeIII GGCC 2 cut(s) 232, 965
HapII CCGG 6 cut(s) 50, 61, 288, 752, 2278, 2368
HgaI GACGC 1 cut(s) 1073
HhaI GCGC 3 cut(s) 170, 1200, 1371
Hin1I GRCGYC 1 cut(s) 2005
Hin1II CATG 7 cut(s) 330, 485, 1051, 1250, 1702, 1877, 2018
Hin6I GCGC 3 cut(s) 168, 1198, 1369
HinP1I GCGC 3 cut(s) 168, 1198, 1369
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 3 cut(s) 1454, 2072, 2298
HinfI GANTC 5 cut(s) 221, 683, 1533, 1562, 1816
HpaII CCGG 6 cut(s) 50, 61, 288, 752, 2278, 2368
HphI GGTGA 3 cut(s) 93, 1509, 2192
Hpy166II GTNNAC 5 cut(s) 118, 1241, 1487, 1583, 1996
Hpy8I GTNNAC 5 cut(s) 118, 1241, 1487, 1583, 1996
Hpy99I CGWCG 2 cut(s) 116, 1067
HpyAV CCTTC 8 cut(s) 67, 71, 328, 335, 418, 1642, 1769, 1994
HpyCH4III ACNGT 9 cut(s) 313, 504, 742, 1269, 1277, 1353, 1392, 1881, 2144
HpyCH4IV ACGT 8 cut(s) 114, 159, 601, 814, 1237, 1422, 1493, 2005
HpyF10VI GCNNNNNNNGC 7 cut(s) 578, 1019, 1044, 1594, 1842, 1929, 2071
HpyF3I CTNAG 7 cut(s) 263, 413, 980, 1457, 1481, 1560, 1822
HpySE526I ACGT 8 cut(s) 114, 159, 601, 814, 1237, 1422, 1493, 2005
Hsp92I GRCGYC 1 cut(s) 2005
Hsp92II CATG 7 cut(s) 330, 485, 1051, 1250, 1702, 1877, 2018
HspAI GCGC 3 cut(s) 168, 1198, 1369
KflI GGGWCCC 1 cut(s) 748
Kpn2I TCCGGA 1 cut(s) 60
Ksp22I TGATCA 1 cut(s) 2325
LguI GCTCTTC 3 cut(s) 1143, 1191, 1497
Lsp1109I GCAGC 3 cut(s) 931, 1062, 1625
LweI GCATC 3 cut(s) 1597, 1981, 2262
MabI ACCWGGT 1 cut(s) 636
MaeI CTAG 5 cut(s) 74, 105, 207, 801, 1784
MaeII ACGT 8 cut(s) 114, 159, 601, 814, 1237, 1422, 1493, 2005
MaeIII GTNAC 7 cut(s) 52, 99, 290, 736, 1160, 2177, 2363
MbiI CCGCTC 2 cut(s) 6, 1803
MflI RGATCY 4 cut(s) 80, 540, 832, 2239
MhlI GDGCHC 4 cut(s) 190, 967, 1155, 1585
MlyI GAGTC 2 cut(s) 1542, 1571
MmeI TCCRAC 9 cut(s) 135, 198, 224, 227, 477, 486, 873, 2241, 2262
MroI TCCGGA 1 cut(s) 60
MroXI GAANNNNTTC 2 cut(s) 14, 1104
MseI TTAA 7 cut(s) 284, 375, 576, 809, 1347, 1644, 1739
MslI CAYNNNNRTG 3 cut(s) 486, 1393, 1866
MspA1I CMGCKG 1 cut(s) 1319
MspI CCGG 6 cut(s) 50, 61, 288, 752, 2278, 2368
Mva1269I GAATGC 1 cut(s) 1785
MvaI CCWGG 8 cut(s) 465, 478, 569, 638, 680, 1530, 2267, 2318
MvnI CGCG 2 cut(s) 131, 1428
MwoI GCNNNNNNNGC 7 cut(s) 578, 1019, 1044, 1594, 1842, 1929, 2071
NciI CCSGG 2 cut(s) 50, 752
NdeI CATATG 2 cut(s) 1114, 1258
NlaIII CATG 7 cut(s) 330, 485, 1051, 1250, 1702, 1877, 2018
NlaIV GGNNCC 8 cut(s) 82, 474, 749, 750, 965, 1527, 2215, 2241
NmuCI GTSAC 3 cut(s) 99, 290, 736
NsbI TGCGCA 1 cut(s) 169
NspV TTCGAA 1 cut(s) 660
OliI CACNNNNGTG 1 cut(s) 1393
PagI TCATGA 1 cut(s) 1246
PciSI GCTCTTC 3 cut(s) 1143, 1191, 1497
PctI GAATGC 1 cut(s) 1785
PdmI GAANNNNTTC 2 cut(s) 14, 1104
PfeI GAWTC 3 cut(s) 221, 683, 1816
Pfl23II CGTACG 1 cut(s) 1423
PfoI TCCNGGA 5 cut(s) 463, 678, 1528, 2265, 2316
PinAI ACCGGT 2 cut(s) 287, 2367
PkrI GCNGC 4 cut(s) 921, 1077, 1430, 1615
Ple19I CGATCG 1 cut(s) 446
PleI GAGTC 2 cut(s) 1541, 1570
PmaCI CACGTG 1 cut(s) 815
PmlI CACGTG 1 cut(s) 815
PpsI GAGTC 2 cut(s) 1541, 1570
Ppu21I YACGTR 3 cut(s) 160, 815, 1494
PpuMI RGGWCCY 2 cut(s) 472, 748
PsiI TTATAA 1 cut(s) 1916
Psp124BI GAGCTC 1 cut(s) 1155
Psp5II RGGWCCY 2 cut(s) 472, 748
Psp6I CCWGG 8 cut(s) 463, 476, 567, 636, 678, 1528, 2265, 2316
PspCI CACGTG 1 cut(s) 815
PspEI GGTNACC 1 cut(s) 52
PspGI CCWGG 8 cut(s) 463, 476, 567, 636, 678, 1528, 2265, 2316
PspLI CGTACG 1 cut(s) 1423
PspN4I GGNNCC 8 cut(s) 82, 474, 749, 750, 965, 1527, 2215, 2241
PspOMI GGGCCC 1 cut(s) 963
PspPI GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1877
PspPPI RGGWCCY 2 cut(s) 472, 748
PstI CTGCAG 1 cut(s) 1854
PsuI RGATCY 4 cut(s) 80, 540, 832, 2239
PvuI CGATCG 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 1319
RsaI GTAC 6 cut(s) 158, 176, 268, 1279, 1425, 2120
RsaNI GTAC 6 cut(s) 157, 175, 267, 1278, 1424, 2119
RseI CAYNNNNRTG 3 cut(s) 486, 1393, 1866
SacI GAGCTC 1 cut(s) 1155
SapI GCTCTTC 3 cut(s) 1143, 1191, 1497
SaqAI TTAA 7 cut(s) 284, 375, 576, 809, 1347, 1644, 1739
SatI GCNGC 4 cut(s) 920, 1076, 1429, 1614
Sau96I GGNCC 7 cut(s) 230, 472, 562, 748, 963, 964, 1877
ScaI AGTACT 1 cut(s) 2120
SchI GAGTC 2 cut(s) 1542, 1571
SduI GDGCHC 4 cut(s) 190, 967, 1155, 1585
SexAI ACCWGGT 1 cut(s) 636
SfaNI GCATC 3 cut(s) 1597, 1981, 2262
SfcI CTRYAG 2 cut(s) 652, 1850
SfuI TTCGAA 1 cut(s) 660
SinI GGWCC 4 cut(s) 472, 562, 748, 1877
SmiMI CAYNNNNRTG 3 cut(s) 486, 1393, 1866
SmlI CTYRAG 1 cut(s) 2258
SmoI CTYRAG 1 cut(s) 2258
SnaBI TACGTA 1 cut(s) 1494
SsiI CCGC 9 cut(s) 6, 19, 129, 565, 1428, 1663, 1801, 2063, 2210
SspI AATATT 1 cut(s) 1572
SspMI CTAG 5 cut(s) 74, 105, 207, 801, 1784
SstI GAGCTC 1 cut(s) 1155
TaaI ACNGT 9 cut(s) 313, 504, 742, 1269, 1277, 1353, 1392, 1881, 2144
TaiI ACGT 8 cut(s) 117, 162, 604, 817, 1240, 1425, 1496, 2008
TaqI TCGA 8 cut(s) 66, 442, 645, 660, 863, 1189, 1705, 1728
TatI WGTACW 2 cut(s) 174, 2118
TauI GCSGC 1 cut(s) 1431
TfiI GAWTC 3 cut(s) 221, 683, 1816
Tru1I TTAA 7 cut(s) 284, 375, 576, 809, 1347, 1644, 1739
Tru9I TTAA 7 cut(s) 284, 375, 576, 809, 1347, 1644, 1739
TscAI CASTG 5 cut(s) 1129, 1163, 1527, 1549, 1590
TseFI GTSAC 3 cut(s) 99, 290, 736
TseI GCWGC 3 cut(s) 919, 1075, 1613
Tsp45I GTSAC 3 cut(s) 99, 290, 736
TspGWI ACGGA 1 cut(s) 180
TspRI CASTG 5 cut(s) 1129, 1163, 1527, 1549, 1590
VneI GTGCAC 1 cut(s) 1581
VpaK11BI GGWCC 4 cut(s) 472, 562, 748, 1877
XagI CCTNNNNNAGG 1 cut(s) 2247
XapI RAATTY 4 cut(s) 694, 932, 1287, 1856
XcmI CCANNNNNNNNNTGG 2 cut(s) 240, 2021
XmnI GAANNNNTTC 2 cut(s) 14, 1104
XspI CTAG 5 cut(s) 74, 105, 207, 801, 1784
ZraI GACGTC 1 cut(s) 2006
ZrmI AGTACT 1 cut(s) 2120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.