Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr13

Orthogroup ID

OG0004734

Physical Location & Seq

Genomic Coordinates Forward (+)

8015422 .. 8019268

Gene Structure 3847 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2232 bp

ATGATTTTCATTTTTGAATCACTCGATTGCGCAAATAACTGGCATAGAACGTTGAGTTTGGCATTTCAGAGCATTGGGATTGTGTATGGGGACATAGGAACGTCTCCGCTCTATGTGTATGCTAGCACTTTCCCTCATGCTATTGACAACAATGACGACATTATAGGGGTACTGTCTCTCATTATCTATACCATAGCGCTCCTGCCTCTACTTAAGTATGCCTTCATCGTGCTATGGGCTAATGACAACGGTGATGGAGGGACATTTGCATTGTATTCCTTGATGTGCCGACATGCAAAGGTGAGCTTAATTCCAAATAACCAACCAGAAGACAGGGAGCTATCAAACTACAAACTTGAAACACCTTCCAACGAGTTAAAACGATCCCAAACCATCAAGAAGAAGCTCGAGAACAGTAAAATTGCAAAATACATCCTTTTCCTTGTCACCATTATGGGAACTTCAATGGTTATTGGAGATGGGGTTCTTACTCCTTGCATTTCAGTCCTTTCTGCCGTGGGCGGGATCAAGTCATTAGGCACAGATGCTGTCGTGGGGATTTCTATAGCAATCCTGATCATACTCTTTTCTGCTCAAAGATTTGGCACTGACAAAGTGGGATTCACCTTTGCACCTATTATCCTTGTGTGGTTCATGTTCATCGGTGGCATTGGACTTTACAATTTATTCAAACATGACATTAGTGTATTACGTGCTTTCAATCCAAAATATATCATCGATTTCTTTAGACGAAATGGGAAAAAGGGATGGATTTCCCTTGGGGGAATATTCCTCTGCATTACTGGGACCGAGGCCATGTTTGCTGATCTGGGTCACTTCAGTGTCAAGGCAATCCAAGTGAGTTTCTCTTGCATCACATTTCCGGCGTTAGTACTTGCATATAGTGGGCAAGCAGCGTTCCTCAGAAAGTTCCCAGGGAAGGTGGACGAGACATTCTATGACTCAATACCAGACCCTTTGTACTGGCCAACGTTCGTTGTGGCAGTAGCAGCTGCCATCATTGCCAGCCAAGCTATGATATCTGGAGCATTTTCAATTATCTCTCAATCCCTGAGTTTGGGTTGTTTTCCAAGAGTTAAGGTTGTTCATACCTCTGCTAAGTATGAGGGCCAAGTATACATACCTGAGATCAACTACATGCTCATGGTTGCTTGTGTTATAGTCACTGCAGCCTTTAAGACCACAGAAAAAATTGGTAATGCATATGGAATTGCAGTGGTTAGTGTAATGGTGATAACAACATGTTTGCTTACTCTGATAATGTTGGTGATATGGAAGACTAGCATATGGTTGATTGGTCTGTTCTTTGTGGTGTTCTTTTGTGTCGAGGTGGTTTATGCATCTGCGGTGATGTACAAATTCGTTCAAGGCGGTTTCCTTCCTCTGGTCTTTTCTTTTTTCCTTATGGTAATCATGGGGATTTGGCATTATGTGCACCATCAAAGATACATGTTCGAGCTAAAGAATAAGGTTTCTAGTGAATACATGAAACAATTGGCCTCTAACCCCAACATAAACCGTGTGCCAGGAATTGGACTTCTATATTCTGAGCTTGTGCAAGGTATTCCCCCTATATTTTCTCACTTTGTTAACAACATACCGTCCATCCACTCTGTTATAGTGATTGTGTCGATCAAGCCTATTCCATTCAGCAAAGTCGCATTGGAGGAAAGGTTTCTATTTCGACAGTTGGAGCCAAGAGACTACCGAATGTTCCGTTGTGTCGCTAGGTATGGTTACAATGATAGAATGGGGGAACCCAAAGAGTTTGAGGAACAACTAGTTGAGCATTTGAAAGAATTTATCCGCCATGAGCACTTCGTACTTGAAGGAGAAATAACAAACGAGGCTGTTTCAACTGGACAGTCATCGAATGCGGAAGAATCAGCTCAACAAGTTAACCAGCCTCGTGGTTCATCAGGGCGCATTCTATCATTTAATACTGCTGGATCAACATCTAACGGAATAGTCTGTGCACCCGTCAAAGGAGCTGCAGAGGAAACGCAGTTCGTGCAAGAGGCCATGGAGAAAGGCATAGTTTATCTGCTGGGACAGACGGAAGTGGTGGCAGGATCAAACTCGTCATTGTTCAAGAGGATAGTGGTCAACCATGTCTACAGTTTCTTGAGGAAAAACTTTAGGCAAGGGGAGCAAGTCATGAGAATCCCACGGACTAGGCTTCTCAGGGTTGGAATGACCTATGAAATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

744

Amino Acids

82.93

Weight (kDa)

8.72

Isoelectric Point (pI)

27.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	20 - 502	6.9e-167	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	516 - 743	1e-25	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004734)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13420
fragaria_vesca	FvH4_4g22240 FvH4_4g22240 FvH4_4g22260
malus_domestica	MD13G1133100.v1.1 MD13G1133200.v1.1 MD16G1143900.v1.1
prunus_persica	Prupe.1G212100_v2.0.a1 Prupe.1G212200_v2.0.a1
pyrus_communis	pycom13g11630
rosa_chinensis	RchiOBHm_Chr4g0428481 RchiOBHm_Chr4g0428501
rosa_laevigata	RLG00000007145 RLG00000007146
rosa_multiflora	Rmu_sc0002072.1_g000037 Rmu_sc0004623.1_g000012 Rmu_sc0006844.1_g000008
rosa_roxburghii	Rroxscaffold_5G00370180 Rroxscaffold_5G00370210
rosa_rugosa	Rorug04G0225500 Rorug04G0225600
rosa_samantha	Rh4AG283100 Rh4AG283200 Rh4BG288800 Rh4BG288900 Rh4CG304400 Rh4CG304900 Rh4DG285900 Rh4DG286000
rosa_wichuraiana	Rw4G024580 Rw4G024600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	31
AccB7I	CCANNNNNTGG	1 cut(s)	1553
AccBSI	CCGCTC	1 cut(s)	109
AccI	GTMKAC	2 cut(s)	1137, 2136
AciI	CCGC	6 cut(s)	107, 522, 1367, 1392, 1828, 1898
AclI	AACGTT	2 cut(s)	50, 992
AclWI	GGATC	4 cut(s)	378, 533, 1978, 2101
AcoI	YGGCCR	1 cut(s)	986
AcsI	RAATTY	2 cut(s)	1379, 1820
AcuI	CTGAAG	1 cut(s)	823
AfaI	GTAC	5 cut(s)	171, 894, 983, 1376, 1845
AfeI	AGCGCT	1 cut(s)	198
AfiI	CCNNNNNNNGG	6 cut(s)	522, 940, 1078, 1405, 1553, 2006
AflII	CTTAAG	1 cut(s)	212
AflIII	ACRYGT	2 cut(s)	1262, 1470
AhlI	ACTAGT	1 cut(s)	1801
AjnI	CCWGG	2 cut(s)	934, 1546
AleI	CACNNNNGTG	1 cut(s)	840
AloI	GAACNNNNNNTCC	4 cut(s)	468, 500, 2012, 2044
AluBI	AGCT	9 cut(s)	306, 340, 406, 1013, 1034, 1480, 1573, 1910, 2012
AluI	AGCT	9 cut(s)	306, 340, 406, 1013, 1034, 1480, 1573, 1910, 2012
Alw21I	GWGCWC	3 cut(s)	1458, 1839, 1999
Alw26I	GTCTC	4 cut(s)	108, 180, 944, 1716
Alw44I	GTGCAC	2 cut(s)	1454, 1995
AlwI	GGATC	4 cut(s)	378, 533, 1978, 2101
AlwNI	CAGNNNCTG	1 cut(s)	548
Ama87I	CYCGRG	1 cut(s)	407
Aor51HI	AGCGCT	1 cut(s)	198
AoxI	GGCC	5 cut(s)	813, 986, 1129, 1518, 2040
ApaLI	GTGCAC	2 cut(s)	1454, 1995
ApeKI	GCWGC	5 cut(s)	914, 1010, 1013, 1190, 2012
ApoI	RAATTY	2 cut(s)	1379, 1820
Asp700I	GAANNNNTTC	1 cut(s)	1695
AspLEI	GCGC	3 cut(s)	32, 199, 1947
AspS9I	GGNCC	2 cut(s)	807, 1129
AsuHPI	GGTGA	7 cut(s)	263, 313, 439, 616, 1264, 1300, 1381
AsuNHI	GCTAGC	1 cut(s)	122
AvaI	CYCGRG	1 cut(s)	407
AvaII	GGWCC	1 cut(s)	807
BaeGI	GKGCMC	2 cut(s)	1458, 1999
BalI	TGGCCA	1 cut(s)	988
BauI	CACGAG	1 cut(s)	1929
BbsI	GAAGAC	2 cut(s)	336, 1304
Bbv12I	GWGCWC	3 cut(s)	1458, 1839, 1999
BbvI	GCAGC	5 cut(s)	926, 1000, 1022, 1202, 1999
BccI	CCATC	7 cut(s)	248, 401, 473, 762, 1025, 1467, 1634
BceAI	ACGGC	1 cut(s)	500
BciT130I	CCWGG	2 cut(s)	936, 1548
BclI	TGATCA	1 cut(s)	576
BcoDI	GTCTC	4 cut(s)	108, 180, 944, 1716
BcuI	ACTAGT	1 cut(s)	1801
BfaI	CTAG	6 cut(s)	123, 1302, 1497, 1749, 1802, 2196
BfmI	CTRYAG	4 cut(s)	564, 1188, 2013, 2137
BfoI	RGCGCY	1 cut(s)	200
BfrI	CTTAAG	1 cut(s)	212
BisI	GCNGC	5 cut(s)	915, 1011, 1014, 1191, 2013
BlsI	GCNGC	5 cut(s)	916, 1012, 1015, 1192, 2014
BmcAI	AGTACT	1 cut(s)	894
Bme1390I	CCNGG	2 cut(s)	936, 1548
Bme18I	GGWCC	1 cut(s)	807
BmeT110I	CYCGRG	1 cut(s)	407
BmgT120I	GGNCC	2 cut(s)	807, 1129
BmiI	GGNNCC	3 cut(s)	808, 1716, 1779
BmrFI	CCNGG	2 cut(s)	936, 1548
BmrI	ACTGGG	1 cut(s)	813
BmsI	GCATC	3 cut(s)	535, 882, 1370
BmtI	GCTAGC	1 cut(s)	126
BmuI	ACTGGG	1 cut(s)	813
BpiI	GAAGAC	2 cut(s)	336, 1304
BpmI	CTGGAG	1 cut(s)	1065
BpuEI	CTTGAG	1 cut(s)	2167
Bsa29I	ATCGAT	1 cut(s)	738
BsaAI	YACGTR	1 cut(s)	713
BsaBI	GATNNNNATC	1 cut(s)	1975
BsaJI	CCNNGG	7 cut(s)	516, 778, 810, 934, 935, 2043, 2189
BsaXI	ACNNNNNCTCC	4 cut(s)	468, 498, 1679, 1709
Bsc4I	CCNNNNNNNGG	6 cut(s)	522, 940, 1078, 1405, 1553, 2006
Bse1I	ACTGG	4 cut(s)	44, 808, 989, 1885
Bse3DI	GCAATG	1 cut(s)	1020
Bse8I	GATNNNNATC	1 cut(s)	1975
BseBI	CCWGG	2 cut(s)	936, 1548
BseCI	ATCGAT	1 cut(s)	738
BseDI	CCNNGG	7 cut(s)	516, 778, 810, 934, 935, 2043, 2189
BseGI	GGATG	3 cut(s)	432, 773, 1626
BseJI	GATNNNNATC	1 cut(s)	1975
BseLI	CCNNNNNNNGG	6 cut(s)	522, 940, 1078, 1405, 1553, 2006
BseMI	GCAATG	1 cut(s)	1020
BseMII	CTCAG	5 cut(s)	937, 1064, 1137, 1560, 2218
BseNI	ACTGG	4 cut(s)	44, 808, 989, 1885
BseSI	GKGCMC	2 cut(s)	1458, 1999
BseXI	GCAGC	5 cut(s)	926, 1000, 1022, 1202, 1999
BseYI	CCCAGC	1 cut(s)	2068
BshFI	GGCC	5 cut(s)	815, 988, 1131, 1520, 2042
BshVI	ATCGAT	1 cut(s)	738
BsiHKAI	GWGCWC	3 cut(s)	1458, 1839, 1999
BsiHKCI	CYCGRG	1 cut(s)	407
BsiSI	CCGG	1 cut(s)	884
BslFI	GGGAC	4 cut(s)	104, 274, 820, 2085
BslI	CCNNNNNNNGG	6 cut(s)	522, 940, 1078, 1405, 1553, 2006
BsmAI	GTCTC	4 cut(s)	108, 180, 944, 1716
BsmBI	CGTCTC	1 cut(s)	108
BsmFI	GGGAC	4 cut(s)	104, 274, 820, 2085
BsmI	GAATGC	2 cut(s)	1900, 1947
BsnI	GGCC	5 cut(s)	815, 988, 1131, 1520, 2042
BsoBI	CYCGRG	1 cut(s)	407
Bsp1286I	GDGCHC	3 cut(s)	1458, 1839, 1999
Bsp1407I	TGTACA	1 cut(s)	1374
Bsp143I	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
Bsp19I	CCATGG	1 cut(s)	2043
BspACI	CCGC	6 cut(s)	107, 522, 1367, 1392, 1828, 1898
BspANI	GGCC	5 cut(s)	815, 988, 1131, 1520, 2042
BspCNI	CTCAG	5 cut(s)	936, 1065, 1138, 1561, 2217
BspDI	ATCGAT	1 cut(s)	738
BspHI	TCATGA	1 cut(s)	2178
BspLI	GGNNCC	3 cut(s)	808, 1716, 1779
BspMAI	CTGCAG	2 cut(s)	1192, 2017
BspOI	GCTAGC	1 cut(s)	126
BspPI	GGATC	4 cut(s)	378, 533, 1978, 2101
BspTI	CTTAAG	1 cut(s)	212
BsrBI	CCGCTC	1 cut(s)	109
BsrDI	GCAATG	1 cut(s)	1020
BsrGI	TGTACA	1 cut(s)	1374
BsrI	ACTGG	4 cut(s)	44, 808, 989, 1885
BssECI	CCNNGG	7 cut(s)	516, 778, 810, 934, 935, 2043, 2189
BssMI	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
BssNAI	GTATAC	1 cut(s)	1138
BssSI	CACGAG	1 cut(s)	1929
BssT1I	CCWWGG	2 cut(s)	778, 2043
Bst1107I	GTATAC	1 cut(s)	1138
Bst2BI	CACGAG	1 cut(s)	1929
Bst2UI	CCWGG	2 cut(s)	936, 1548
Bst4CI	ACNGT	8 cut(s)	174, 251, 416, 1541, 1623, 1710, 1887, 2141
BstAFI	CTTAAG	1 cut(s)	212
BstAPI	GCANNNNNTGC	2 cut(s)	1453, 2032
BstAUI	TGTACA	1 cut(s)	1374
BstBAI	YACGTR	1 cut(s)	713
BstC8I	GCNNGC	3 cut(s)	124, 912, 1027
BstDEI	CTNAG	6 cut(s)	923, 1073, 1119, 1146, 1569, 2204
BstDSI	CCRYGG	3 cut(s)	516, 2043, 2189
BstF5I	GGATG	3 cut(s)	432, 773, 1626
BstH2I	RGCGCY	1 cut(s)	200
BstHHI	GCGC	3 cut(s)	32, 199, 1947
BstKTI	GATC	8 cut(s)	386, 528, 579, 829, 1152, 1656, 1973, 2096
BstMAI	GTCTC	4 cut(s)	108, 180, 944, 1716
BstMBI	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
BstMWI	GCNNNNNNNGC	7 cut(s)	821, 1010, 1022, 1031, 1453, 2032, 2170
BstNI	CCWGG	2 cut(s)	936, 1548
BstNSI	RCATGY	4 cut(s)	296, 1162, 1266, 1474
BstSCI	CCNGG	2 cut(s)	934, 1546
BstSFI	CTRYAG	4 cut(s)	564, 1188, 2013, 2137
BstSLI	GKGCMC	2 cut(s)	1458, 1999
BstV1I	GCAGC	5 cut(s)	926, 1000, 1022, 1202, 1999
BstV2I	GAAGAC	2 cut(s)	336, 1304
BstXI	CCANNNNNNTGG	1 cut(s)	1931
BstZ17I	GTATAC	1 cut(s)	1138
Bsu15I	ATCGAT	1 cut(s)	738
BsuRI	GGCC	5 cut(s)	815, 988, 1131, 1520, 2042
BsuTUI	ATCGAT	1 cut(s)	738
BtgI	CCRYGG	3 cut(s)	516, 2043, 2189
BtsCI	GGATG	3 cut(s)	432, 773, 1626
BtsI	GCAGTG	2 cut(s)	1185, 1242
BtsIMutI	CAGTG	4 cut(s)	606, 847, 1185, 1242
Cac8I	GCNNGC	3 cut(s)	124, 912, 1027
CaiI	CAGNNNCTG	1 cut(s)	548
CciI	TCATGA	1 cut(s)	2178
CfoI	GCGC	3 cut(s)	32, 199, 1947
Cfr13I	GGNCC	2 cut(s)	807, 1129
ClaI	ATCGAT	1 cut(s)	738
Csp6I	GTAC	5 cut(s)	170, 893, 982, 1375, 1844
CviQI	GTAC	5 cut(s)	170, 893, 982, 1375, 1844
DdeI	CTNAG	6 cut(s)	923, 1073, 1119, 1146, 1569, 2204
DpnI	GATC	8 cut(s)	385, 527, 578, 828, 1151, 1655, 1972, 2095
DpnII	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
EaeI	YGGCCR	1 cut(s)	986
EciI	GGCGGA	1 cut(s)	1817
Eco130I	CCWWGG	2 cut(s)	778, 2043
Eco32I	GATATC	1 cut(s)	1041
Eco47I	GGWCC	1 cut(s)	807
Eco47III	AGCGCT	1 cut(s)	198
Eco57I	CTGAAG	1 cut(s)	823
Eco88I	CYCGRG	1 cut(s)	407
EcoRII	CCWGG	2 cut(s)	934, 1546
EcoRV	GATATC	1 cut(s)	1041
EcoT14I	CCWWGG	2 cut(s)	778, 2043
EcoT22I	ATGCAT	2 cut(s)	1225, 1363
ErhI	CCWWGG	2 cut(s)	778, 2043
Esp3I	CGTCTC	1 cut(s)	108
FalI	AAGNNNNNCTT	4 cut(s)	206, 238, 290, 322
FaqI	GGGAC	4 cut(s)	104, 274, 820, 2085
FauI	CCCGC	1 cut(s)	515
FauNDI	CATATG	2 cut(s)	1225, 1307
FbaI	TGATCA	1 cut(s)	576
FblI	GTMKAC	2 cut(s)	1137, 2136
Fnu4HI	GCNGC	5 cut(s)	915, 1011, 1014, 1191, 2013
FokI	GGATG	3 cut(s)	419, 780, 1613
Fsp4HI	GCNGC	5 cut(s)	915, 1011, 1014, 1191, 2013
FspBI	CTAG	6 cut(s)	123, 1302, 1497, 1749, 1802, 2196
FspI	TGCGCA	1 cut(s)	31
GlaI	GCGC	3 cut(s)	31, 198, 1946
GluI	GCNGC	5 cut(s)	915, 1011, 1014, 1191, 2013
GsaI	CCCAGC	1 cut(s)	2072
GsuI	CTGGAG	1 cut(s)	1065
HaeII	RGCGCY	1 cut(s)	200
HaeIII	GGCC	5 cut(s)	815, 988, 1131, 1520, 2042
HapII	CCGG	1 cut(s)	884
HhaI	GCGC	3 cut(s)	32, 199, 1947
Hin6I	GCGC	3 cut(s)	30, 197, 1945
HinP1I	GCGC	3 cut(s)	30, 197, 1945
HincII	GTYRAC	3 cut(s)	1612, 1921, 2128
HindII	GTYRAC	3 cut(s)	1612, 1921, 2128
HinfI	GANTC	5 cut(s)	17, 621, 962, 1904, 2184
HpaI	GTTAAC	2 cut(s)	1612, 1921
HpaII	CCGG	1 cut(s)	884
HphI	GGTGA	7 cut(s)	263, 313, 439, 616, 1264, 1300, 1381
Hpy166II	GTNNAC	8 cut(s)	946, 1138, 1456, 1612, 1921, 1997, 2128, 2137
Hpy188I	TCNGA	4 cut(s)	69, 926, 1278, 1570
Hpy188III	TCNNGA	7 cut(s)	397, 409, 574, 1044, 2113, 2146, 2179
Hpy8I	GTNNAC	8 cut(s)	946, 1138, 1456, 1612, 1921, 1997, 2128, 2137
HpyAV	CCTTC	5 cut(s)	232, 375, 934, 1409, 1844
HpyCH4III	ACNGT	8 cut(s)	174, 251, 416, 1541, 1623, 1710, 1887, 2141
HpyCH4IV	ACGT	4 cut(s)	50, 101, 712, 992
HpyF10VI	GCNNNNNNNGC	7 cut(s)	821, 1010, 1022, 1031, 1453, 2032, 2170
HpyF3I	CTNAG	6 cut(s)	923, 1073, 1119, 1146, 1569, 2204
HpySE526I	ACGT	4 cut(s)	50, 101, 712, 992
HspAI	GCGC	3 cut(s)	30, 197, 1945
Ksp22I	TGATCA	1 cut(s)	576
KspAI	GTTAAC	2 cut(s)	1612, 1921
Kzo9I	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
LmnI	GCTCC	6 cut(s)	204, 337, 1046, 1714, 2009, 2170
Lsp1109I	GCAGC	5 cut(s)	926, 1000, 1022, 1202, 1999
LweI	GCATC	3 cut(s)	535, 882, 1370
MaeI	CTAG	6 cut(s)	123, 1302, 1497, 1749, 1802, 2196
MaeII	ACGT	4 cut(s)	50, 101, 712, 992
MaeIII	GTNAC	4 cut(s)	445, 833, 1183, 1757
MalI	GATC	8 cut(s)	385, 527, 578, 828, 1151, 1655, 1972, 2095
MbiI	CCGCTC	1 cut(s)	109
MboI	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
MboII	GAAGA	4 cut(s)	341, 412, 1309, 1913
MfeI	CAATTG	1 cut(s)	1514
MhlI	GDGCHC	3 cut(s)	1458, 1839, 1999
MlsI	TGGCCA	1 cut(s)	988
MluNI	TGGCCA	1 cut(s)	988
MlyI	GAGTC	1 cut(s)	956
MmeI	TCCRAC	3 cut(s)	393, 1692, 2191
Mox20I	TGGCCA	1 cut(s)	988
Mph1103I	ATGCAT	2 cut(s)	1225, 1363
MroXI	GAANNNNTTC	1 cut(s)	1695
MscI	TGGCCA	1 cut(s)	988
MseI	TTAA	8 cut(s)	213, 308, 377, 1098, 1197, 1611, 1920, 1959
MslI	CAYNNNNRTG	3 cut(s)	452, 840, 1163
Msp20I	TGGCCA	1 cut(s)	988
MspA1I	CMGCKG	1 cut(s)	1013
MspCI	CTTAAG	1 cut(s)	212
MspI	CCGG	1 cut(s)	884
MspR9I	CCNGG	2 cut(s)	936, 1548
MunI	CAATTG	1 cut(s)	1514
Mva1269I	GAATGC	2 cut(s)	1900, 1947
MvaI	CCWGG	2 cut(s)	936, 1548
MwoI	GCNNNNNNNGC	7 cut(s)	821, 1010, 1022, 1031, 1453, 2032, 2170
NcoI	CCATGG	1 cut(s)	2043
NdeI	CATATG	2 cut(s)	1225, 1307
NdeII	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
NheI	GCTAGC	1 cut(s)	122
NlaIV	GGNNCC	3 cut(s)	808, 1716, 1779
NmuCI	GTSAC	3 cut(s)	445, 833, 1183
NsbI	TGCGCA	1 cut(s)	31
NsiI	ATGCAT	2 cut(s)	1225, 1363
NspI	RCATGY	4 cut(s)	296, 1162, 1266, 1474
OliI	CACNNNNGTG	1 cut(s)	840
PaeR7I	CTCGAG	1 cut(s)	407
PagI	TCATGA	1 cut(s)	2178
PasI	CCCWGGG	1 cut(s)	935
PciI	ACATGT	2 cut(s)	1262, 1470
PctI	GAATGC	2 cut(s)	1900, 1947
PdmI	GAANNNNTTC	1 cut(s)	1695
PfeI	GAWTC	4 cut(s)	17, 621, 1904, 2184
PflMI	CCANNNNNTGG	1 cut(s)	1553
PkrI	GCNGC	5 cut(s)	916, 1012, 1015, 1192, 2014
PleI	GAGTC	1 cut(s)	956
PpsI	GAGTC	1 cut(s)	956
Ppu21I	YACGTR	1 cut(s)	713
PscI	ACATGT	2 cut(s)	1262, 1470
Psp1406I	AACGTT	2 cut(s)	50, 992
Psp6I	CCWGG	2 cut(s)	934, 1546
PspFI	CCCAGC	1 cut(s)	2068
PspGI	CCWGG	2 cut(s)	934, 1546
PspN4I	GGNNCC	3 cut(s)	808, 1716, 1779
PspPI	GGNCC	2 cut(s)	807, 1129
PstI	CTGCAG	2 cut(s)	1192, 2017
PstNI	CAGNNNCTG	1 cut(s)	548
PvuII	CAGCTG	1 cut(s)	1013
RsaI	GTAC	5 cut(s)	171, 894, 983, 1376, 1845
RsaNI	GTAC	5 cut(s)	170, 893, 982, 1375, 1844
RseI	CAYNNNNRTG	3 cut(s)	452, 840, 1163
SaqAI	TTAA	8 cut(s)	213, 308, 377, 1098, 1197, 1611, 1920, 1959
SatI	GCNGC	5 cut(s)	915, 1011, 1014, 1191, 2013
Sau3AI	GATC	8 cut(s)	383, 525, 576, 826, 1149, 1653, 1970, 2093
Sau96I	GGNCC	2 cut(s)	807, 1129
ScaI	AGTACT	1 cut(s)	894
SchI	GAGTC	1 cut(s)	956
ScrFI	CCNGG	2 cut(s)	936, 1548
SduI	GDGCHC	3 cut(s)	1458, 1839, 1999
SfaNI	GCATC	3 cut(s)	535, 882, 1370
SfcI	CTRYAG	4 cut(s)	564, 1188, 2013, 2137
Sfr274I	CTCGAG	1 cut(s)	407
SinI	GGWCC	1 cut(s)	807
SlaI	CTCGAG	1 cut(s)	407
SmiMI	CAYNNNNRTG	3 cut(s)	452, 840, 1163
SmlI	CTYRAG	3 cut(s)	212, 407, 2146
SmoI	CTYRAG	3 cut(s)	212, 407, 2146
SpeI	ACTAGT	1 cut(s)	1801
SsiI	CCGC	6 cut(s)	107, 522, 1367, 1392, 1828, 1898
SspI	AATATT	1 cut(s)	789
SspMI	CTAG	6 cut(s)	123, 1302, 1497, 1749, 1802, 2196
StyD4I	CCNGG	2 cut(s)	934, 1546
StyI	CCWWGG	2 cut(s)	778, 2043
TaaI	ACNGT	8 cut(s)	174, 251, 416, 1541, 1623, 1710, 1887, 2141
TaiI	ACGT	4 cut(s)	53, 104, 715, 995
TaqI	TCGA	8 cut(s)	24, 408, 738, 1347, 1476, 1652, 1705, 1892
TaqII	GACCGA	1 cut(s)	824
TatI	WGTACW	3 cut(s)	892, 981, 1374
TfiI	GAWTC	4 cut(s)	17, 621, 1904, 2184
Tru1I	TTAA	8 cut(s)	213, 308, 377, 1098, 1197, 1611, 1920, 1959
Tru9I	TTAA	8 cut(s)	213, 308, 377, 1098, 1197, 1611, 1920, 1959
TscAI	CASTG	4 cut(s)	613, 847, 1192, 1242
TseFI	GTSAC	3 cut(s)	445, 833, 1183
TseI	GCWGC	5 cut(s)	914, 1010, 1013, 1190, 2012
Tsp45I	GTSAC	3 cut(s)	445, 833, 1183
TspDTI	ATGAA	6 cut(s)	214, 643, 649, 1097, 1523, 1926
TspGWI	ACGGA	4 cut(s)	1727, 1998, 2093, 2206
TspRI	CASTG	4 cut(s)	613, 847, 1192, 1242
Van91I	CCANNNNNTGG	1 cut(s)	1553
Vha464I	CTTAAG	1 cut(s)	212
VneI	GTGCAC	2 cut(s)	1454, 1995
VpaK11BI	GGWCC	1 cut(s)	807
XapI	RAATTY	2 cut(s)	1379, 1820
XceI	RCATGY	4 cut(s)	296, 1162, 1266, 1474
XhoI	CTCGAG	1 cut(s)	407
XmiI	GTMKAC	2 cut(s)	1137, 2136
XmnI	GAANNNNTTC	1 cut(s)	1695
XspI	CTAG	6 cut(s)	123, 1302, 1497, 1749, 1802, 2196
ZrmI	AGTACT	1 cut(s)	894
Zsp2I	ATGCAT	2 cut(s)	1225, 1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.