pycom13g13250

pectinesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015910
Physical Location & Seq
Forward (+)
9359957 .. 9360817
861 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ATGGATCCAAGGTGCTATACTCAAACGCCTCTCATCATCTTATTCTTCTTCATTTTCGGCTTTAACTCCGCGCAAGCTGTATGCAAGCCCCGGGATTTGAAACTGAGGTACCTAACTATTCTCTCTCCATCACCAAGTTCTCCTAACTCGCAGGCTTCCGTGCCTCCTAGTAAGCCACATCCGGGTCAAGTCTTCCCTTCGTCGCGGCCAGCAGGCCCTCCACCAACCCGTAGTCCTACTCCGGCCCCAACTCCCTCCCCAAACCCTCCTTCACAGCCTCCACAAGCATCACCCCCTGCTGGCAATTCAATTTCTCCATCTTCCTCCTCATCCTTTCTTCGCCGTTCTTCTGACAGCCCACGCGGAAACCTCTTTGCGCAGCCTTTTGTTCTTAACACGGCCTCATCATCAACCCCACCTAACCCGGCGATCCAAGAACTATGCAAAAACACCGACTACCCTGATCTTTGCATCTTATCCCTGACCCCTTTCCAACCACCCAAGGTTGACCCCGTATCCGCTCTTGGGATGACAATCAAGGCTTGCACCGCACACGCCCAGATGTCTCTAGCCGCAGCCTCAAAACTTGTGACGATGCCTAACACTGCACGTGGAACGATTGCTGCGCTCAGTGATTGCAAGGACTTGTACTCTGATGCCTTGGACGGCCTTCAGAGCGCCATTGATGCAATCCAGAGCCACGACATTGGCACGATTAATAGCATGCTTAGCGGGGTGGTGACCGATGCTGTGACGTGTGGGGATGGGTTTGAAGGAGAGCAATCTCCGCTGGGTGATTACGATGACAAACTTCGTAAAATGGCCAGCAATTGCCTTGCCATTGCTTCCTTGATCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

287

Amino Acids

30.04

Weight (kDa)

7.53

Isoelectric Point (pI)

68.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 143 - 281 2e-29 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015910)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G36659
fragaria_vesca FvH4_4g19570
malus_domestica MD16G1155100.v1.1
prunus_persica Prupe.1G118800_v2.0.a1
pyrus_communis pycom13g13250
rosa_chinensis RchiOBHm_Chr4g0424831
rosa_laevigata RLG00000007417
rosa_multiflora Rmu_sc0003608.1_g000023
rosa_roxburghii Rroxscaffold_5G00367050
rosa_rugosa Rorug04G0199400
rosa_samantha Rh4AG257200 Rh4BG262500 Rh4CG273500 Rh4DG258300
rosa_wichuraiana Rw4G022190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 378
Acc65I GGTACC 1 cut(s) 108
AccB1I GGYRCC 1 cut(s) 108
AccBSI CCGCTC 1 cut(s) 521
AccII CGCG 3 cut(s) 71, 205, 363
AciI CCGC 8 cut(s) 69, 205, 363, 519, 549, 573, 732, 788
AclWI GGATC 2 cut(s) 12, 424
AcoI YGGCCR 2 cut(s) 206, 822
AcuI CTGAAG 1 cut(s) 656
AcvI CACGTG 1 cut(s) 611
AfaI GTAC 2 cut(s) 110, 650
AfiI CCNNNNNNNGG 3 cut(s) 182, 299, 525
AflIII ACRYGT 1 cut(s) 755
AgsI TTSAA 3 cut(s) 100, 309, 773
AjiI CACGTC 1 cut(s) 756
AjuI GAANNNNNNNTTGG 2 cut(s) 253, 285
AluBI AGCT 1 cut(s) 77
AluI AGCT 1 cut(s) 77
Alw26I GTCTC 1 cut(s) 570
AlwI GGATC 2 cut(s) 12, 424
Ama87I CYCGRG 1 cut(s) 90
AoxI GGCC 6 cut(s) 206, 214, 243, 399, 667, 822
ApeKI GCWGC 3 cut(s) 379, 575, 623
AseI ATTAAT 1 cut(s) 717
Asp718I GGTACC 1 cut(s) 108
AspLEI GCGC 4 cut(s) 73, 379, 628, 680
AspS9I GGNCC 2 cut(s) 215, 244
AsuC2I CCSGG 4 cut(s) 91, 92, 183, 425
AsuHPI GGTGA 4 cut(s) 123, 282, 751, 806
AvaI CYCGRG 1 cut(s) 90
BaeI ACNNNNGTAYC 2 cut(s) 92, 125
BalI TGGCCA 1 cut(s) 824
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 108
BbrPI CACGTG 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 184
BbvI GCAGC 3 cut(s) 391, 587, 610
BccI CCATC 3 cut(s) 136, 325, 758
BceAI ACGGC 3 cut(s) 327, 414, 682
BciVI GTATCC 1 cut(s) 526
BclI TGATCA 1 cut(s) 852
BcnI CCSGG 4 cut(s) 91, 92, 183, 425
BcoDI GTCTC 1 cut(s) 570
BfaI CTAG 2 cut(s) 168, 569
BfoI RGCGCY 1 cut(s) 681
BfuI GTATCC 1 cut(s) 526
BisI GCNGC 5 cut(s) 206, 380, 573, 576, 624
BlpI GCTNAGC 1 cut(s) 728
BlsI GCNGC 5 cut(s) 207, 381, 574, 577, 625
Bme1390I CCNGG 4 cut(s) 91, 92, 183, 425
BmeT110I CYCGRG 1 cut(s) 90
BmgBI CACGTC 1 cut(s) 756
BmgT120I GGNCC 2 cut(s) 215, 244
BmiI GGNNCC 3 cut(s) 6, 110, 246
BmrFI CCNGG 4 cut(s) 91, 92, 183, 425
BmsI GCATC 6 cut(s) 296, 480, 585, 646, 676, 736
BpiI GAAGAC 1 cut(s) 184
Bpu1102I GCTNAGC 1 cut(s) 728
BpuMI CCSGG 4 cut(s) 91, 92, 183, 425
BsaAI YACGTR 1 cut(s) 611
BsaBI GATNNNNATC 1 cut(s) 533
BsaJI CCNNGG 5 cut(s) 8, 89, 90, 501, 660
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 299, 525
Bse3DI GCAATG 1 cut(s) 840
Bse8I GATNNNNATC 1 cut(s) 533
BseDI CCNNGG 5 cut(s) 8, 89, 90, 501, 660
BseGI GGATG 4 cut(s) 178, 329, 534, 769
BseJI GATNNNNATC 1 cut(s) 533
BseLI CCNNNNNNNGG 3 cut(s) 182, 299, 525
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 2 cut(s) 95, 643
BseRI GAGGAG 1 cut(s) 316
BseXI GCAGC 3 cut(s) 391, 587, 610
BseYI CCCAGC 1 cut(s) 790
BsgI GTGCAG 1 cut(s) 591
Bsh1236I CGCG 3 cut(s) 71, 205, 363
BshFI GGCC 6 cut(s) 208, 216, 245, 401, 669, 824
BshNI GGYRCC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 4 cut(s) 91, 182, 242, 425
BslI CCNNNNNNNGG 3 cut(s) 182, 299, 525
BsmAI GTCTC 1 cut(s) 570
BsnI GGCC 6 cut(s) 208, 216, 245, 401, 669, 824
BsoBI CYCGRG 1 cut(s) 90
Bsp143I GATC 4 cut(s) 4, 429, 463, 852
Bsp1720I GCTNAGC 1 cut(s) 728
BspACI CCGC 8 cut(s) 69, 205, 363, 519, 549, 573, 732, 788
BspANI GGCC 6 cut(s) 208, 216, 245, 401, 669, 824
BspCNI CTCAG 2 cut(s) 96, 642
BspFNI CGCG 3 cut(s) 71, 205, 363
BspLI GGNNCC 3 cut(s) 6, 110, 246
BspPI GGATC 2 cut(s) 12, 424
BspT107I GGYRCC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 521
BsrDI GCAATG 1 cut(s) 840
BssECI CCNNGG 5 cut(s) 8, 89, 90, 501, 660
BssMI GATC 4 cut(s) 4, 429, 463, 852
BssT1I CCWWGG 3 cut(s) 8, 501, 660
BstBAI YACGTR 1 cut(s) 611
BstC8I GCNNGC 9 cut(s) 75, 86, 153, 210, 214, 301, 544, 725, 826
BstDEI CTNAG 3 cut(s) 104, 629, 728
BstEII GGTNACC 1 cut(s) 739
BstF5I GGATG 4 cut(s) 178, 329, 534, 769
BstFNI CGCG 3 cut(s) 71, 205, 363
BstH2I RGCGCY 1 cut(s) 681
BstHHI GCGC 4 cut(s) 73, 379, 628, 680
BstKTI GATC 4 cut(s) 7, 432, 466, 855
BstMAI GTCTC 1 cut(s) 570
BstMBI GATC 4 cut(s) 4, 429, 463, 852
BstMWI GCNNNNNNNGC 5 cut(s) 548, 675, 686, 729, 787
BstNSI RCATGY 1 cut(s) 727
BstPI GGTNACC 1 cut(s) 739
BstSCI CCNGG 4 cut(s) 89, 90, 181, 423
BstUI CGCG 3 cut(s) 71, 205, 363
BstV1I GCAGC 3 cut(s) 391, 587, 610
BstV2I GAAGAC 1 cut(s) 184
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 707
BstYI RGATCY 1 cut(s) 4
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 6 cut(s) 208, 216, 245, 401, 669, 824
BtrI CACGTC 1 cut(s) 756
BtsCI GGATG 4 cut(s) 178, 329, 534, 769
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 2 cut(s) 603, 637
Cac8I GCNNGC 9 cut(s) 75, 86, 153, 210, 214, 301, 544, 725, 826
CfoI GCGC 4 cut(s) 73, 379, 628, 680
Cfr13I GGNCC 2 cut(s) 215, 244
Cfr9I CCCGGG 1 cut(s) 90
Csp6I GTAC 2 cut(s) 109, 649
CviAII CATG 1 cut(s) 724
CviQI GTAC 2 cut(s) 109, 649
DdeI CTNAG 3 cut(s) 104, 629, 728
DpnI GATC 4 cut(s) 6, 431, 465, 854
DpnII GATC 4 cut(s) 4, 429, 463, 852
EaeI YGGCCR 2 cut(s) 206, 822
Eco130I CCWWGG 3 cut(s) 8, 501, 660
Eco57I CTGAAG 1 cut(s) 656
Eco72I CACGTG 1 cut(s) 611
Eco88I CYCGRG 1 cut(s) 90
Eco91I GGTNACC 1 cut(s) 739
EcoO109I RGGNCCY 1 cut(s) 215
EcoO65I GGTNACC 1 cut(s) 739
EcoT14I CCWWGG 3 cut(s) 8, 501, 660
ErhI CCWWGG 3 cut(s) 8, 501, 660
FaeI CATG 1 cut(s) 727
FaiI YATR 4 cut(s) 18, 82, 442, 725
FatI CATG 1 cut(s) 723
FauI CCCGC 1 cut(s) 725
FbaI TGATCA 1 cut(s) 852
Fnu4HI GCNGC 5 cut(s) 206, 380, 573, 576, 624
FokI GGATG 4 cut(s) 165, 316, 541, 776
Fsp4HI GCNGC 5 cut(s) 206, 380, 573, 576, 624
FspBI CTAG 2 cut(s) 168, 569
FspI TGCGCA 1 cut(s) 378
GlaI GCGC 4 cut(s) 72, 378, 627, 679
GluI GCNGC 5 cut(s) 206, 380, 573, 576, 624
GsaI CCCAGC 1 cut(s) 794
HaeII RGCGCY 1 cut(s) 681
HaeIII GGCC 6 cut(s) 208, 216, 245, 401, 669, 824
HapII CCGG 4 cut(s) 91, 182, 242, 425
HhaI GCGC 4 cut(s) 73, 379, 628, 680
Hin1II CATG 1 cut(s) 727
Hin6I GCGC 4 cut(s) 71, 377, 626, 678
HinP1I GCGC 4 cut(s) 71, 377, 626, 678
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HpaII CCGG 4 cut(s) 91, 182, 242, 425
HphI GGTGA 4 cut(s) 123, 282, 751, 806
Hpy166II GTNNAC 1 cut(s) 508
Hpy188I TCNGA 3 cut(s) 352, 655, 675
Hpy188III TCNNGA 1 cut(s) 694
Hpy8I GTNNAC 1 cut(s) 508
Hpy99I CGWCG 1 cut(s) 205
HpyAV CCTTC 4 cut(s) 207, 279, 680, 767
HpyCH4IV ACGT 2 cut(s) 610, 755
HpyCH4V TGCA 7 cut(s) 84, 444, 471, 546, 608, 639, 689
HpyF10VI GCNNNNNNNGC 5 cut(s) 548, 675, 686, 729, 787
HpyF3I CTNAG 3 cut(s) 104, 629, 728
HpySE526I ACGT 2 cut(s) 610, 755
Hsp92II CATG 1 cut(s) 727
HspAI GCGC 4 cut(s) 71, 377, 626, 678
KpnI GGTACC 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 852
Kzo9I GATC 4 cut(s) 4, 429, 463, 852
Lsp1109I GCAGC 3 cut(s) 391, 587, 610
LweI GCATC 6 cut(s) 296, 480, 585, 646, 676, 736
MaeI CTAG 2 cut(s) 168, 569
MaeII ACGT 2 cut(s) 610, 755
MaeIII GTNAC 3 cut(s) 589, 739, 751
MalI GATC 4 cut(s) 6, 431, 465, 854
MbiI CCGCTC 1 cut(s) 521
MboI GATC 4 cut(s) 4, 429, 463, 852
MboII GAAGA 6 cut(s) 37, 40, 184, 312, 329, 339
MfeI CAATTG 1 cut(s) 829
MflI RGATCY 1 cut(s) 4
MlsI TGGCCA 1 cut(s) 824
MluCI AATT 3 cut(s) 304, 309, 829
MluNI TGGCCA 1 cut(s) 824
MmeI TCCRAC 1 cut(s) 517
Mox20I TGGCCA 1 cut(s) 824
MscI TGGCCA 1 cut(s) 824
MseI TTAA 3 cut(s) 63, 393, 717
Msp20I TGGCCA 1 cut(s) 824
MspA1I CMGCKG 1 cut(s) 790
MspI CCGG 4 cut(s) 91, 182, 242, 425
MspR9I CCNGG 4 cut(s) 91, 92, 183, 425
MunI CAATTG 1 cut(s) 829
MvnI CGCG 3 cut(s) 71, 205, 363
MwoI GCNNNNNNNGC 5 cut(s) 548, 675, 686, 729, 787
NciI CCSGG 4 cut(s) 91, 92, 183, 425
NdeII GATC 4 cut(s) 4, 429, 463, 852
NlaIII CATG 1 cut(s) 727
NlaIV GGNNCC 3 cut(s) 6, 110, 246
NmuCI GTSAC 3 cut(s) 589, 739, 751
NsbI TGCGCA 1 cut(s) 378
NspI RCATGY 1 cut(s) 727
PaeI GCATGC 1 cut(s) 727
PkrI GCNGC 5 cut(s) 207, 381, 574, 577, 625
PmaCI CACGTG 1 cut(s) 611
PmlI CACGTG 1 cut(s) 611
Ppu21I YACGTR 1 cut(s) 611
PshBI ATTAAT 1 cut(s) 717
PspCI CACGTG 1 cut(s) 611
PspEI GGTNACC 1 cut(s) 739
PspFI CCCAGC 1 cut(s) 790
PspN4I GGNNCC 3 cut(s) 6, 110, 246
PspPI GGNCC 2 cut(s) 215, 244
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 110, 650
RsaNI GTAC 2 cut(s) 109, 649
SaqAI TTAA 3 cut(s) 63, 393, 717
SatI GCNGC 5 cut(s) 206, 380, 573, 576, 624
Sau3AI GATC 4 cut(s) 4, 429, 463, 852
Sau96I GGNCC 2 cut(s) 215, 244
ScrFI CCNGG 4 cut(s) 91, 92, 183, 425
SetI ASST 9 cut(s) 14, 79, 110, 114, 372, 421, 507, 613, 758
SfaNI GCATC 6 cut(s) 296, 480, 585, 646, 676, 736
SmaI CCCGGG 1 cut(s) 92
SphI GCATGC 1 cut(s) 727
Sse9I AATT 3 cut(s) 304, 309, 829
SsiI CCGC 8 cut(s) 69, 205, 363, 519, 549, 573, 732, 788
SspMI CTAG 2 cut(s) 168, 569
StyD4I CCNGG 4 cut(s) 89, 90, 181, 423
StyI CCWWGG 3 cut(s) 8, 501, 660
TaiI ACGT 2 cut(s) 613, 758
TaqII GACCGA 1 cut(s) 758
TasI AATT 3 cut(s) 304, 309, 829
TatI WGTACW 1 cut(s) 648
TauI GCSGC 2 cut(s) 208, 575
Tru1I TTAA 3 cut(s) 63, 393, 717
Tru9I TTAA 3 cut(s) 63, 393, 717
TscAI CASTG 2 cut(s) 610, 637
TseFI GTSAC 3 cut(s) 589, 739, 751
TseI GCWGC 3 cut(s) 379, 575, 623
Tsp45I GTSAC 3 cut(s) 589, 739, 751
TspDTI ATGAA 1 cut(s) 40
TspGWI ACGGA 1 cut(s) 148
TspMI CCCGGG 1 cut(s) 90
TspRI CASTG 2 cut(s) 610, 637
VspI ATTAAT 1 cut(s) 717
XceI RCATGY 1 cut(s) 727
XmaI CCCGGG 1 cut(s) 90
XspI CTAG 2 cut(s) 168, 569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.