pycom13g15420

SAC3/GANP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0007937
Physical Location & Seq
Forward (+)
11230555 .. 11240305
9751 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4731 bp
ATGTACCCGGGATTTGGCAAGGCGTCAGGACCAACAGGACCGCCGGGATCGCAGCCACTTTTCGGAAACCTTGGCGGCGGTCGGCCGCCTTCGCCTTCTCCGCCGACCACCACTCCTTTTTCCGGCGCTGCTCCGCTGCGTTCTCCGTCCAGGGGGCCTGAAGCTCCAGGGCAGCTGTATTCTCTTCCTTTAGGCTTTGAAAGAGTTCGTCCTGCCGCGAGTCAACCTTTCCCATCTGCGGGAGTCTATAGGCGGCCTGAATCTTCAGGGCAGCTGCATTCACCTCCTTTGGCTTTTGAAAGAGCTCGTCCTGCTGCAAGTCATCCTCACCCATCTGCAGGAGTTCATAGGTCCACAGAGTCTCATCCTACTTGGGATCATGGGCAGAGATCCTCCTTTAAGAATTATGATACCCAAGCTCATCAAAGGCCATCTGCTGTCACATCATTTGTTGTTTCTCGTAATTCTGGAACTAGTGTTACTGCCAAGGTGTCCAGATTCCAAGACACTGAAGGGATCAGGTCGCCTCCCTTTCTGTCTAAAGATGTCAATTTTAGAAATTCCACTCAAGGTGCCCCCCGAAGTCACTTAGTTGCTCCAAGAATACGATCACCTCCCTTGGTCTCTTATGAGGATTTGCATCCTTCCGTTGGAGTTGAAGAGCCTTCTGCATCTTCTGTTGGGTTCAATACTAGATCAAATACCAATTTTAGTACTCCTGATTCTCGGGTACATCAAAAGTCTTTGCAATCTGCAAGCAATACTATTTCTGAAGCTGCTGCAGGAAATGCGACCAACATCCCTGTGGCCAAAAGGACGAGGTCACCTCCTTTACTTCCGGAGGATCAAGTGTTTCATGGAAACTCTCATGCCACTCAAGATGGTACTGAACGAGAAATGCAAGCCAAAGCTAAGCGGTTAGCCCGGTTCAAGGTTGAATTAAGTAAAACTCCACAAAACAATCCTGATAATGTAGAACCAGGGGTTTCTGCAAATAGACGTGAGCAATCTAATGTTGAGAGGAACAAATTAGCTGCATATAAATCTACCGAACTGGCTATGGGTGGTAGTGATGGCAATGCATTACCTGAGGATGAAGGCGTGGAGTCATCTGGTATCATTATTGGGTTGTGCCCGGATATGTGTCCAGAGTCAGAAAGGGCAGAACGAGAAAGGAAAGGGGATCTTGATCAATATGAGCGCTTGGATGGGGATAGAAATCAAACCAGCATGTCCCTTGCTGTTAAAAAGTATAACAGAACAGCTGAGCGAGATGCAAACCTGATAAGACCAATGCCAGTCCTGCAGAAGACAATTGATTATTTGCTCAATTTGCTTGATCAGCCTTACAATGATAGGTTTCTTAGAATTTACAACTTCCTGTGGGATCGGATGCGAGCAATTCGAATGGACTTGAGAATGCAGCACATTTTTAACCAAGAGGCTATTACTATGCTGGAGCAAATGATAAGACTTCACATAATTGTCATGCATGAATTATGTGAATACTCAAGAGGAGAAGGCTTTGCTGAGGGATTTGATGCACACCTCAATATCGAACAGATGAATAAAACATCTGTTGAATTGTTCCAATTGTATGATGATCACAGAAAGAAAGGAATAAACATTCCTACAGAAAAAGAGTTTCGAGGTTATTATGCCCTTCTCAAGCTGGACAAGCATCCTGGGTACATGGTTGAACCTGCAGAGCTCTCGCTTGATCTTGCAAAGATGACTCCAGAAATAAGACAAACTTCAGAGGTGCTATTTGCACGTGATGTCGCTAGAGCTTGCAGAACTGGTAATTTTATTGCTTTTTTTCGACTTGCAAGGAAAGCAAGTTACCTCCAATCATGCCTAATGCATGCTCACTTCTCAAAGTTACGAACCCAGGCACTTGCTTCTATACACGCAGGTCTCCAGAATAACCAAGGTCTCCCTATTGCGGATGGTGCCAAGTGGCTGGCAATGGAGGAAGATGAAATTGAAAGCCTTTCAGAGTATCATGGGTTTGTAATAAAGTCATTCCAGGAGCCATATATTGTGAAGGAAGGTCCATTTCTCAATGGTGATGAGGATTATCCTACCAAGTGTTCTAAACTTGTCGATATGAAAAAGTCAAGAAGGATAGTAGAGGATGTTTTGGCTTCTAGTCAAGTCATACCCTCGTCTTCTAAAGCAACAAAAAAGACTCAGTTGACTAAGTCAAATAAGCCTGAACCAAAAACTATTTCATATGTTGAAAAGAAAAGTCCTGTGCGTCGTGCTCCAGCCATTGAAGTTACAAAACCTCTCCATGAAGTTGATGAAGAAATGCCTAATTTTGAAGCTTTTTCACCCCCAAAAGATGCCAGACAAAAGCAGCAAATGATTCAAGCACCAATCTTCTCATCTCCGGAAGATTCCAGACAAAACCAGCAAACGATTCAAACACCAAATTTTGGTATATACAGTGAAGATTCCCCTAAGGTGGCTGCTGTGTCTCCTTCCCCGTGGGGTTTCTCATCATTTACACGTCAGCCGGATAAAGTTGGTACCATTGAGAAGCGAAATTATGATGTTCTTTTTAGTAACTCTCCAGAGAGAAGCATGCATTCTGGCATGGAACGAATGCCACTGCAAATTGAGTCAAAAACATCTGTACAAGAGAGATCTGTTGGTATATATAGTTCCGGTAAGGAATATCCAGATATCCAAATTATGGTCACTGATAACTTGGAAGGCGAGGAACCTTCAGATCTTCATCAGATAGATGAAAACAATGATGTTATGGAGAGTTCTCAACAGGAAGAAATTGCAGAGGCAAAACTTAAGTTGATACTGAGATTATGGAAGCGGCGTGCTCTGAAGCTAAGACAATTGCGAGAGAAAAAGCAGTTGGCAGCAAATGCTGCATTGAATTCTTTATCATTGGGGCCGCCAATTCAACTCAAGACAGATCAACCAAGCACTTCTCGCGAGTTTGACATTGATCTCGTCTTGAGGGAGAGATTTAAAAACCATGGTCGGTCATGGTCAAGACTTAATGTTTCAGATGTAATAGCAGATATACTGGGCACAAGAAACCCGGATGCTAGATGCCTCTGTTGGAAAACTGTTGTATGTTCTCAGATGAACAATCTAGAAGGTGATGACCTGGGGCAGAGGAGCCACTTCTTGGGAGCAGCCCCCTGGTTGCTTTCAAAACTCATGCCTTCGGCAAATGATGCGGACAATGATGATGAGGATCTCGTAGTTTCGTCTCCTGGTGTCTCTATATGGAAGAAATGGGTGCGTGGCCAAGCTGGTTCAGATCTGAACTGCTATTTGTCTGTGGTCAAGGATGCAAGTTTTGATAACCTAAGTCAGAGTGTGTCTGGTGCAAGTGCTGTTCTGTTCCTTACATCTGAAAGTATCTCTTGGAATTTGCAAAAAGTCCGTCTTCATAACCTTCTAATGTTGATACCTTACGGCTCCTGCTTGCCCCTTCTGATCTTAAGTGGCTCGTACAAGAACAATGTTGATGACTCCACTATTGTTGATAATTTGGGTCTCCGTGACTTGGATAAGTCACGAATAAGCAGCTTTCTGGTTGTTCCGCTTGTTGAAAGCCAGCAAATGGGACAATTGGATGGTTTTTTCAGTGACAACCAACTTAGGGAGGGGCTTCGATGGCTTGCAAGTGAATCGCCCCTTCAACCTATTCTTCATCATGTAAAAACACTCGAACTCGTTCTTACCCACTTGAATTCTTCATTGGAGCCACTAGACAGAATGAAAGATTATGAAGCAGGTCCAGACAACTGTATCCTTGCCTTCAACGAAGCATTGGATTGGTCACAGAAGGAAATTGCTGCTGCTGTCAAATCAAATCCATCCAGTTGGCCTTGCCCCGAGATTGCTTTGCTGGAGGAGTTCAGTGATGAGTACAGGGTTGTGAAATGGTGCTTGCCAAGCGTAGGATGGAGCTCAGCGGAAAAAGTTGAACCACTAATGTCAGCTCTAGGGGATTGTAGGCTTCCAGCTTTTCCCGACCATATTTCGTGGCTACCCCAATGTTGTAATGCAGGAAAGGAGATTGAGAACCTGAGAGGTGAACTTGAAAATGGCTTGATCGAATACCTGACGAATTCAAGTAAGATGATGGGACGTGCACTTGCTATAAAAGAGGCACATGTAATGCTACAAAGAAGTTGTCGACTGCAGCGCCATGACTCGTGCTGCTATATAGTCCCGAATTGGGTTATGATCTTCAGACGAATTTTCAACTGGCGGTTAATGGGGTTAGCCAATGGGGCCTCTTCGTCAGCTTATGTCTTGGAGAGTCCTCATCTTAACGCAGTTTTTGAAAATCTGGGTAAGTTGGGGCTTGAAGATGCCGGACCTTCACCCTGTTACCTTAATCAGCCGTCTCTGGATGAAATCATCGAAGTTGGGCGCGCCCCTCTTTCATCCCATAGAGGTCAGCCCTTGGTAGAATCTGGTGTAGCCGTTCCTGAAACATCTCCTAATGGTGAAATCTACGAGACCCCGAATTCGACTGACTGGATGGAGGATGAACGAAGTTTAGCGCACGATGATCAAGCGGAAATAGAAAACGTATCCCACGAAAATGGGAGATTGGAGAATGCTGGCGGAGAAATGGTGGTGACTGGAGAAGTGGGCAAGGGGGCTGAAAAATTAAGCATATTGTTAGAACAGTGTGACATGTTACAGAATGTGATAGACGAGAAGCTGTCCATTTTTTTCTAA

Protein Analysis

1577

Amino Acids

175.72

Weight (kDa)

5.87

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAC3_GANP PF03399 381 - 674 1.3e-81 SAC3/GANP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1711, 1904, 3761
Acc65I GGTACC 1 cut(s) 2534
AccB1I GGYRCC 3 cut(s) 572, 1952, 2534
AccB7I CCANNNNNTGG 4 cut(s) 2441, 2701, 3157, 3598
AccI GTMKAC 1 cut(s) 4177
AccII CGCG 3 cut(s) 218, 2958, 4419
AccIII TCCGGA 2 cut(s) 838, 2395
AclWI GGATC 8 cut(s) 55, 384, 384, 524, 852, 1191, 1395, 3234
AcoI YGGCCR 3 cut(s) 83, 807, 3277
AcsI RAATTY 9 cut(s) 559, 1368, 2437, 2899, 3403, 3727, 4108, 4239, 4513
AcuI CTGAAG 8 cut(s) 180, 249, 531, 792, 1740, 2718, 2867, 4216
AcvI CACGTG 1 cut(s) 1775
AcyI GRCGYC 1 cut(s) 23
AfaI GTAC 9 cut(s) 5, 715, 732, 886, 1691, 2536, 2643, 3488, 3908
AfeI AGCGCT 1 cut(s) 1202
AflII CTTAAG 2 cut(s) 2810, 3475
AflIII ACRYGT 3 cut(s) 2513, 4153, 4686
AhlI ACTAGT 1 cut(s) 473
AjiI CACGTC 3 cut(s) 1001, 2516, 4130
AjnI CCWGG 9 cut(s) 149, 166, 979, 1684, 1890, 2028, 3135, 3170, 3244
AjuI GAANNNNNNNTTGG 6 cut(s) 2215, 2247, 2861, 2893, 3719, 3751
Alw21I GWGCWC 6 cut(s) 307, 1713, 2269, 2845, 3950, 4135
Alw44I GTGCAC 1 cut(s) 4131
AlwI GGATC 8 cut(s) 55, 384, 384, 524, 852, 1191, 1395, 3234
Ama87I CYCGRG 3 cut(s) 7, 726, 3872
Aor13HI TCCGGA 2 cut(s) 838, 2395
Aor51HI AGCGCT 1 cut(s) 1202
AoxI GGCC 9 cut(s) 83, 155, 254, 428, 807, 2915, 3277, 3863, 4275
ApaLI GTGCAC 1 cut(s) 4131
ApoI RAATTY 9 cut(s) 559, 1368, 2437, 2899, 3403, 3727, 4108, 4239, 4513
ArsI GACNNNNNNTTYG 6 cut(s) 3403, 3435, 4159, 4191, 4498, 4530
AscI GGCGCGCC 1 cut(s) 4417
Asp700I GAANNNNTTC 2 cut(s) 204, 3711
Asp718I GGTACC 1 cut(s) 2534
AspLEI GCGC 6 cut(s) 128, 1203, 4188, 4419, 4421, 4552
AspS9I GGNCC 9 cut(s) 29, 38, 155, 351, 2054, 2915, 3773, 4275, 4361
AsuC2I CCSGG 6 cut(s) 8, 9, 45, 925, 1136, 3068
AsuII TTCGAA 1 cut(s) 1405
AvaI CYCGRG 3 cut(s) 7, 726, 3872
AvaII GGWCC 6 cut(s) 29, 38, 351, 2054, 3773, 4361
AxyI CCTNAGG 2 cut(s) 1089, 2466
BaeGI GKGCMC 4 cut(s) 577, 1136, 3059, 4135
BaeI ACNNNNGTAYC 2 cut(s) 3769, 3802
BalI TGGCCA 2 cut(s) 809, 3279
BanI GGYRCC 3 cut(s) 572, 1952, 2534
BanII GRGCYC 3 cut(s) 307, 1713, 3950
BauI CACGAG 1 cut(s) 4195
BbrPI CACGTG 1 cut(s) 1775
BbsI GAAGAC 3 cut(s) 1316, 2163, 3413
Bbv12I GWGCWC 6 cut(s) 307, 1713, 2269, 2845, 3950, 4135
BbvCI CCTCAGC 1 cut(s) 1530
BceAI ACGGC 3 cut(s) 3466, 4372, 4454
BcgI CGANNNNNNTGC 4 cut(s) 1695, 1729, 3648, 3682
BciT130I CCWGG 9 cut(s) 151, 168, 981, 1686, 1892, 2030, 3137, 3172, 3246
BciVI GTATCC 2 cut(s) 3797, 4591
BclI TGATCA 4 cut(s) 1189, 1339, 1603, 4558
BcnI CCSGG 6 cut(s) 8, 9, 45, 925, 1136, 3068
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 8 cut(s) 474, 693, 1785, 2151, 3075, 3122, 3746, 3983
BfmI CTRYAG 7 cut(s) 247, 336, 780, 1304, 1632, 1704, 4181
BfoI RGCGCY 3 cut(s) 129, 1204, 4189
BfrI CTTAAG 2 cut(s) 2810, 3475
BfuAI ACCTGC 3 cut(s) 1711, 1904, 3761
BfuI GTATCC 2 cut(s) 3797, 4591
BglI GCCNNNNNGGC 1 cut(s) 4274
BglII AGATCT 3 cut(s) 2651, 2737, 3292
BlpI GCTNAGC 3 cut(s) 912, 1266, 3949
BmcAI AGTACT 1 cut(s) 715
Bme18I GGWCC 6 cut(s) 29, 38, 351, 2054, 3773, 4361
BmeT110I CYCGRG 3 cut(s) 7, 726, 3872
BmgBI CACGTC 3 cut(s) 1001, 2516, 4130
BmgT120I GGNCC 9 cut(s) 29, 38, 155, 351, 2054, 2915, 3773, 4275, 4361
BmrI ACTGGG 1 cut(s) 3062
BmuI ACTGGG 1 cut(s) 3062
BpiI GAAGAC 3 cut(s) 1316, 2163, 3413
BplI GAGNNNNNCTC 2 cut(s) 811, 843
BpmI CTGGAG 8 cut(s) 150, 1478, 1722, 1904, 2253, 2562, 3908, 4653
Bpu10I CCTNAGC 1 cut(s) 1530
Bpu1102I GCTNAGC 3 cut(s) 912, 1266, 3949
Bpu14I TTCGAA 1 cut(s) 1405
BpuEI CTTGAG 7 cut(s) 552, 861, 1435, 1495, 1652, 2915, 3001
BpuMI CCSGG 6 cut(s) 8, 9, 45, 925, 1136, 3068
BsaAI YACGTR 1 cut(s) 1775
BsaBI GATNNNNATC 5 cut(s) 639, 1188, 1218, 2742, 3225
BsaHI GRCGYC 1 cut(s) 23
BsaI GGTCTC 5 cut(s) 628, 1922, 1940, 3536, 4499
BsaWI WCCGGW 3 cut(s) 838, 2395, 2672
BsaXI ACNNNNNCTCC 4 cut(s) 97, 127, 4608, 4638
Bse1I ACTGG 8 cut(s) 1059, 1298, 1804, 3057, 3858, 4253, 4529, 4636
Bse21I CCTNAGG 2 cut(s) 1089, 2466
Bse3DI GCAATG 2 cut(s) 1084, 1974
Bse8I GATNNNNATC 5 cut(s) 639, 1188, 1218, 2742, 3225
BseAI TCCGGA 2 cut(s) 838, 2395
BseBI CCWGG 9 cut(s) 151, 168, 981, 1686, 1892, 2030, 3137, 3172, 3246
BseJI GATNNNNATC 5 cut(s) 639, 1188, 1218, 2742, 3225
BseMI GCAATG 2 cut(s) 1084, 1974
BseMII CTCAG 8 cut(s) 1080, 1257, 1521, 2207, 2813, 3122, 3963, 4058
BseNI ACTGG 8 cut(s) 1059, 1298, 1804, 3057, 3858, 4253, 4529, 4636
BsePI GCGCGC 1 cut(s) 4417
BseRI GAGGAG 3 cut(s) 1530, 3160, 3905
BseSI GKGCMC 4 cut(s) 577, 1136, 3059, 4135
BseX3I CGGCCG 1 cut(s) 83
Bsh1236I CGCG 3 cut(s) 218, 2958, 4419
Bsh1285I CGRYCG 2 cut(s) 82, 86
BshFI GGCC 9 cut(s) 85, 157, 256, 430, 809, 2917, 3279, 3865, 4277
BshNI GGYRCC 3 cut(s) 572, 1952, 2534
BsiEI CGRYCG 2 cut(s) 82, 86
BsiHKAI GWGCWC 6 cut(s) 307, 1713, 2269, 2845, 3950, 4135
BsiHKCI CYCGRG 3 cut(s) 7, 726, 3872
BslFI GGGAC 4 cut(s) 1219, 3615, 4140, 4196
BsmBI CGTCTC 2 cut(s) 3246, 4395
BsmFI GGGAC 4 cut(s) 1219, 3615, 4140, 4196
BsmI GAATGC 5 cut(s) 277, 1425, 2593, 2616, 4612
BsnI GGCC 9 cut(s) 85, 157, 256, 430, 809, 2917, 3279, 3865, 4277
Bso31I GGTCTC 5 cut(s) 628, 1922, 1940, 3536, 4499
BsoBI CYCGRG 3 cut(s) 7, 726, 3872
Bsp119I TTCGAA 1 cut(s) 1405
Bsp1286I GDGCHC 9 cut(s) 307, 577, 1136, 1713, 2269, 2845, 3059, 3950, 4135
Bsp13I TCCGGA 2 cut(s) 838, 2395
Bsp1407I TGTACA 1 cut(s) 2641
Bsp1720I GCTNAGC 3 cut(s) 912, 1266, 3949
Bsp19I CCATGG 1 cut(s) 3001
Bsp68I TCGCGA 1 cut(s) 2958
BspANI GGCC 9 cut(s) 85, 157, 256, 430, 809, 2917, 3279, 3865, 4277
BspCNI CTCAG 8 cut(s) 1081, 1258, 1522, 2206, 2814, 3121, 3962, 4059
BspEI TCCGGA 2 cut(s) 838, 2395
BspFNI CGCG 3 cut(s) 218, 2958, 4419
BspMAI CTGCAG 5 cut(s) 340, 784, 1308, 1708, 4185
BspMI ACCTGC 3 cut(s) 1711, 1904, 3761
BspPI GGATC 8 cut(s) 55, 384, 384, 524, 852, 1191, 1395, 3234
BspQI GCTCTTC 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 1405
BspT107I GGYRCC 3 cut(s) 572, 1952, 2534
BspTI CTTAAG 2 cut(s) 2810, 3475
BspTNI GGTCTC 5 cut(s) 628, 1922, 1940, 3536, 4499
BsrDI GCAATG 2 cut(s) 1084, 1974
BsrGI TGTACA 1 cut(s) 2641
BsrI ACTGG 8 cut(s) 1059, 1298, 1804, 3057, 3858, 4253, 4529, 4636
BssHII GCGCGC 1 cut(s) 4417
BssNI GRCGYC 1 cut(s) 23
BssSI CACGAG 1 cut(s) 4195
BssT1I CCWWGG 6 cut(s) 70, 486, 618, 1930, 3001, 4449
Bst2BI CACGAG 1 cut(s) 4195
Bst2UI CCWGG 9 cut(s) 151, 168, 981, 1686, 1892, 2030, 3137, 3172, 3246
Bst4CI ACNGT 4 cut(s) 2453, 3097, 3785, 4680
Bst6I CTCTTC 3 cut(s) 189, 654, 4285
BstACI GRCGYC 1 cut(s) 23
BstAFI CTTAAG 2 cut(s) 2810, 3475
BstAPI GCANNNNNTGC 4 cut(s) 788, 2888, 2891, 3206
BstAUI TGTACA 1 cut(s) 2641
BstBAI YACGTR 1 cut(s) 1775
BstBI TTCGAA 1 cut(s) 1405
BstDSI CCRYGG 2 cut(s) 2492, 3001
BstEII GGTNACC 1 cut(s) 822
BstFNI CGCG 3 cut(s) 218, 2958, 4419
BstH2I RGCGCY 3 cut(s) 129, 1204, 4189
BstHHI GCGC 6 cut(s) 128, 1203, 4188, 4419, 4421, 4552
BstMCI CGRYCG 2 cut(s) 82, 86
BstNI CCWGG 9 cut(s) 151, 168, 981, 1686, 1892, 2030, 3137, 3172, 3246
BstNSI RCATGY 5 cut(s) 1234, 1868, 2593, 4157, 4690
BstPI GGTNACC 1 cut(s) 822
BstSFI CTRYAG 7 cut(s) 247, 336, 780, 1304, 1632, 1704, 4181
BstSLI GKGCMC 4 cut(s) 577, 1136, 3059, 4135
BstUI CGCG 3 cut(s) 218, 2958, 4419
BstV2I GAAGAC 3 cut(s) 1316, 2163, 3413
BstX2I RGATCY 6 cut(s) 389, 1183, 2651, 2737, 3226, 3292
BstXI CCANNNNNNTGG 3 cut(s) 1963, 3861, 4592
BstYI RGATCY 6 cut(s) 389, 1183, 2651, 2737, 3226, 3292
BstZI CGGCCG 1 cut(s) 83
Bsu36I CCTNAGG 2 cut(s) 1089, 2466
BsuI GTATCC 2 cut(s) 3797, 4591
BsuRI GGCC 9 cut(s) 85, 157, 256, 430, 809, 2917, 3279, 3865, 4277
BtgI CCRYGG 2 cut(s) 2492, 3001
BtrI CACGTC 3 cut(s) 1001, 2516, 4130
BtsI GCAGTG 1 cut(s) 2615
BtsIMutI CAGTG 7 cut(s) 507, 2458, 2615, 2706, 3628, 3904, 4685
BtuMI TCGCGA 1 cut(s) 2958
BveI ACCTGC 3 cut(s) 1711, 1904, 3761
CfoI GCGC 6 cut(s) 128, 1203, 4188, 4419, 4421, 4552
Cfr13I GGNCC 9 cut(s) 29, 38, 155, 351, 2054, 2915, 3773, 4275, 4361
Cfr9I CCCGGG 1 cut(s) 7
CseI GACGC 2 cut(s) 12, 2249
Csp6I GTAC 9 cut(s) 4, 714, 731, 885, 1690, 2535, 2642, 3487, 3907
CspCI CAANNNNNGTGG 2 cut(s) 3499, 3534
CviQI GTAC 9 cut(s) 4, 714, 731, 885, 1690, 2535, 2642, 3487, 3907
DraI TTTAAA 1 cut(s) 2995
EaeI YGGCCR 3 cut(s) 83, 807, 3277
EagI CGGCCG 1 cut(s) 83
Eam1104I CTCTTC 3 cut(s) 189, 654, 4285
EarI CTCTTC 3 cut(s) 189, 654, 4285
EciI GGCGGA 2 cut(s) 90, 4629
Ecl136II GAGCTC 3 cut(s) 305, 1711, 3948
EclXI CGGCCG 1 cut(s) 83
Eco130I CCWWGG 6 cut(s) 70, 486, 618, 1930, 3001, 4449
Eco24I GRGCYC 3 cut(s) 307, 1713, 3950
Eco31I GGTCTC 5 cut(s) 628, 1922, 1940, 3536, 4499
Eco32I GATATC 1 cut(s) 2692
Eco47I GGWCC 6 cut(s) 29, 38, 351, 2054, 3773, 4361
Eco47III AGCGCT 1 cut(s) 1202
Eco52I CGGCCG 1 cut(s) 83
Eco53kI GAGCTC 3 cut(s) 305, 1711, 3948
Eco57I CTGAAG 8 cut(s) 180, 249, 531, 792, 1740, 2718, 2867, 4216
Eco72I CACGTG 1 cut(s) 1775
Eco81I CCTNAGG 2 cut(s) 1089, 2466
Eco88I CYCGRG 3 cut(s) 7, 726, 3872
Eco91I GGTNACC 1 cut(s) 822
EcoICRI GAGCTC 3 cut(s) 305, 1711, 3948
EcoO109I RGGNCCY 2 cut(s) 155, 4275
EcoO65I GGTNACC 1 cut(s) 822
EcoRI GAATTC 4 cut(s) 2899, 3727, 4108, 4513
EcoRII CCWGG 9 cut(s) 149, 166, 979, 1684, 1890, 2028, 3135, 3170, 3244
EcoRV GATATC 1 cut(s) 2692
EcoT14I CCWWGG 6 cut(s) 70, 486, 618, 1930, 3001, 4449
EcoT22I ATGCAT 4 cut(s) 1084, 1494, 1866, 2595
EcoT38I GRGCYC 3 cut(s) 307, 1713, 3950
ErhI CCWWGG 6 cut(s) 70, 486, 618, 1930, 3001, 4449
Esp3I CGTCTC 2 cut(s) 3246, 4395
FalI AAGNNNNNCTT 8 cut(s) 1170, 1202, 1738, 1770, 3382, 3414, 3405, 3437
FaqI GGGAC 4 cut(s) 1219, 3615, 4140, 4196
FauI CCCGC 1 cut(s) 232
FauNDI CATATG 1 cut(s) 2236
FbaI TGATCA 4 cut(s) 1189, 1339, 1603, 4558
FblI GTMKAC 1 cut(s) 4177
FriOI GRGCYC 3 cut(s) 307, 1713, 3950
FspBI CTAG 8 cut(s) 474, 693, 1785, 2151, 3075, 3122, 3746, 3983
GlaI GCGC 6 cut(s) 127, 1202, 4187, 4418, 4420, 4551
GsuI CTGGAG 8 cut(s) 150, 1478, 1722, 1904, 2253, 2562, 3908, 4653
HaeII RGCGCY 3 cut(s) 129, 1204, 4189
HaeIII GGCC 9 cut(s) 85, 157, 256, 430, 809, 2917, 3279, 3865, 4277
HgaI GACGC 2 cut(s) 12, 2249
HhaI GCGC 6 cut(s) 128, 1203, 4188, 4419, 4421, 4552
Hin1I GRCGYC 1 cut(s) 23
Hin6I GCGC 6 cut(s) 126, 1201, 4186, 4417, 4419, 4550
HinP1I GCGC 6 cut(s) 126, 1201, 4186, 4417, 4419, 4550
HincII GTYRAC 3 cut(s) 224, 2199, 4178
HindII GTYRAC 3 cut(s) 224, 2199, 4178
HindIII AAGCTT 1 cut(s) 2328
Hpy166II GTNNAC 6 cut(s) 224, 354, 2199, 4076, 4133, 4178
Hpy8I GTNNAC 6 cut(s) 224, 354, 2199, 4076, 4133, 4178
Hpy99I CGWCG 1 cut(s) 2265
HpyCH4III ACNGT 4 cut(s) 2453, 3097, 3785, 4680
HpyCH4IV ACGT 5 cut(s) 1000, 1774, 2515, 4129, 4578
HpySE526I ACGT 5 cut(s) 1000, 1774, 2515, 4129, 4578
Hsp92I GRCGYC 1 cut(s) 23
HspAI GCGC 6 cut(s) 126, 1201, 4186, 4417, 4419, 4550
Kpn2I TCCGGA 2 cut(s) 838, 2395
KpnI GGTACC 1 cut(s) 2538
Ksp22I TGATCA 4 cut(s) 1189, 1339, 1603, 4558
LguI GCTCTTC 1 cut(s) 654
MaeI CTAG 8 cut(s) 474, 693, 1785, 2151, 3075, 3122, 3746, 3983
MaeII ACGT 5 cut(s) 1000, 1774, 2515, 4129, 4578
MfeI CAATTG 4 cut(s) 1314, 1592, 2858, 3605
MflI RGATCY 6 cut(s) 389, 1183, 2651, 2737, 3226, 3292
MhlI GDGCHC 9 cut(s) 307, 577, 1136, 1713, 2269, 2845, 3059, 3950, 4135
MlsI TGGCCA 2 cut(s) 809, 3279
MluNI TGGCCA 2 cut(s) 809, 3279
MmeI TCCRAC 2 cut(s) 631, 3068
Mox20I TGGCCA 2 cut(s) 809, 3279
Mph1103I ATGCAT 4 cut(s) 1084, 1494, 1866, 2595
MroI TCCGGA 2 cut(s) 838, 2395
MroXI GAANNNNTTC 2 cut(s) 204, 3711
MscI TGGCCA 2 cut(s) 809, 3279
MslI CAYNNNNRTG 2 cut(s) 803, 4590
Msp20I TGGCCA 2 cut(s) 809, 3279
MspA1I CMGCKG 5 cut(s) 136, 175, 274, 1265, 3953
MspCI CTTAAG 2 cut(s) 2810, 3475
MunI CAATTG 4 cut(s) 1314, 1592, 2858, 3605
Mva1269I GAATGC 5 cut(s) 277, 1425, 2593, 2616, 4612
MvaI CCWGG 9 cut(s) 151, 168, 981, 1686, 1892, 2030, 3137, 3172, 3246
MvnI CGCG 3 cut(s) 218, 2958, 4419
NciI CCSGG 6 cut(s) 8, 9, 45, 925, 1136, 3068
NcoI CCATGG 1 cut(s) 3001
NdeI CATATG 1 cut(s) 2236
NruI TCGCGA 1 cut(s) 2958
NsiI ATGCAT 4 cut(s) 1084, 1494, 1866, 2595
NspI RCATGY 5 cut(s) 1234, 1868, 2593, 4157, 4690
NspV TTCGAA 1 cut(s) 1405
PaeI GCATGC 2 cut(s) 1868, 2593
PalAI GGCGCGCC 1 cut(s) 4417
PauI GCGCGC 1 cut(s) 4417
PciI ACATGT 2 cut(s) 4153, 4686
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 1 cut(s) 4584
PctI GAATGC 5 cut(s) 277, 1425, 2593, 2616, 4612
PdmI GAANNNNTTC 2 cut(s) 204, 3711
PfeI GAWTC 9 cut(s) 260, 498, 722, 2371, 2402, 2425, 2459, 3665, 4457
PflFI GACNNNGTC 2 cut(s) 820, 2203
PflMI CCANNNNNTGG 4 cut(s) 2441, 2701, 3157, 3598
PfoI TCCNGGA 1 cut(s) 2028
PmaCI CACGTG 1 cut(s) 1775
PmlI CACGTG 1 cut(s) 1775
Ppu21I YACGTR 1 cut(s) 1775
PscI ACATGT 2 cut(s) 4153, 4686
Psp124BI GAGCTC 3 cut(s) 307, 1713, 3950
Psp6I CCWGG 9 cut(s) 149, 166, 979, 1684, 1890, 2028, 3135, 3170, 3244
PspCI CACGTG 1 cut(s) 1775
PspEI GGTNACC 1 cut(s) 822
PspGI CCWGG 9 cut(s) 149, 166, 979, 1684, 1890, 2028, 3135, 3170, 3244
PspPI GGNCC 9 cut(s) 29, 38, 155, 351, 2054, 2915, 3773, 4275, 4361
PsrI GAACNNNNNNTAC 4 cut(s) 463, 495, 2653, 2685
PstI CTGCAG 5 cut(s) 340, 784, 1308, 1708, 4185
PsuI RGATCY 6 cut(s) 389, 1183, 2651, 2737, 3226, 3292
PsyI GACNNNGTC 2 cut(s) 820, 2203
PteI GCGCGC 1 cut(s) 4417
PvuII CAGCTG 3 cut(s) 175, 274, 1265
RruI TCGCGA 1 cut(s) 2958
RsaI GTAC 9 cut(s) 5, 715, 732, 886, 1691, 2536, 2643, 3488, 3908
RsaNI GTAC 9 cut(s) 4, 714, 731, 885, 1690, 2535, 2642, 3487, 3907
RseI CAYNNNNRTG 2 cut(s) 803, 4590
SacI GAGCTC 3 cut(s) 307, 1713, 3950
SalI GTCGAC 1 cut(s) 4176
SapI GCTCTTC 1 cut(s) 654
Sau96I GGNCC 9 cut(s) 29, 38, 155, 351, 2054, 2915, 3773, 4275, 4361
ScaI AGTACT 1 cut(s) 715
SduI GDGCHC 9 cut(s) 307, 577, 1136, 1713, 2269, 2845, 3059, 3950, 4135
SfcI CTRYAG 7 cut(s) 247, 336, 780, 1304, 1632, 1704, 4181
SfuI TTCGAA 1 cut(s) 1405
SgsI GGCGCGCC 1 cut(s) 4417
SinI GGWCC 6 cut(s) 29, 38, 351, 2054, 3773, 4361
SmaI CCCGGG 1 cut(s) 9
SmiMI CAYNNNNRTG 2 cut(s) 803, 4590
SmlI CTYRAG 9 cut(s) 567, 876, 1414, 1510, 1667, 2810, 2930, 2980, 3475
SmoI CTYRAG 9 cut(s) 567, 876, 1414, 1510, 1667, 2810, 2930, 2980, 3475
SpeI ACTAGT 1 cut(s) 473
SphI GCATGC 2 cut(s) 1868, 2593
SspMI CTAG 8 cut(s) 474, 693, 1785, 2151, 3075, 3122, 3746, 3983
SstI GAGCTC 3 cut(s) 307, 1713, 3950
StyI CCWWGG 6 cut(s) 70, 486, 618, 1930, 3001, 4449
TaaI ACNGT 4 cut(s) 2453, 3097, 3785, 4680
TaiI ACGT 5 cut(s) 1003, 1777, 2518, 4132, 4581
TaqII GACCGA 1 cut(s) 2997
TatI WGTACW 3 cut(s) 713, 2641, 3906
TauI GCSGC 6 cut(s) 78, 88, 218, 256, 2839, 2920
TfiI GAWTC 9 cut(s) 260, 498, 722, 2371, 2402, 2425, 2459, 3665, 4457
TscAI CASTG 7 cut(s) 514, 2458, 2622, 2713, 3628, 3904, 4685
TspGWI ACGGA 4 cut(s) 135, 637, 3407, 3524
TspMI CCCGGG 1 cut(s) 7
TspRI CASTG 7 cut(s) 514, 2458, 2622, 2713, 3628, 3904, 4685
Tth111I GACNNNGTC 2 cut(s) 820, 2203
Van91I CCANNNNNTGG 4 cut(s) 2441, 2701, 3157, 3598
Vha464I CTTAAG 2 cut(s) 2810, 3475
VneI GTGCAC 1 cut(s) 4131
VpaK11BI GGWCC 6 cut(s) 29, 38, 351, 2054, 3773, 4361
XapI RAATTY 9 cut(s) 559, 1368, 2437, 2899, 3403, 3727, 4108, 4239, 4513
XbaI TCTAGA 1 cut(s) 3121
XceI RCATGY 5 cut(s) 1234, 1868, 2593, 4157, 4690
XcmI CCANNNNNNNNNTGG 2 cut(s) 802, 3939
XmaI CCCGGG 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 4177
XmnI GAANNNNTTC 2 cut(s) 204, 3711
XspI CTAG 8 cut(s) 474, 693, 1785, 2151, 3075, 3122, 3746, 3983
ZrmI AGTACT 1 cut(s) 715
Zsp2I ATGCAT 4 cut(s) 1084, 1494, 1866, 2595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.