pycom13g16480

CRAL/TRIO domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012073
Physical Location & Seq
Reverse (-)
12265575 .. 12268813
3239 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
ATGGGGAAGAAAGATCACCACCCGAGAGATGAACAGAGCAAAGGAAATGCAAGAGTGGAAGCAGTTCTTCAGCTTCTGAGAAAACAAGCCCCACTCACAGTTAAACAGGAGAAGTTTTGCAACACTGCTTGTGTGGAGAGGTTTTTGAAAGCAAAAGGAGATAGTGTTAAGAAGACGGCCAAGCAACTTAGGGCTTGCCTTTCGTGGAGACAGAATATTGGCACTGAGAACTTGATAGCAGATGAGTTCTCAGCTGAGCTCGCTGAAGGTGTTGCCTACGTGTTCACTCGCTTGTTGGTATTCACGCTGGAGGTGGCGATTCAGTCCATGCCAAAAAACGTAGAGCAGTTCGTCCTCCTTTTCGACGCAAGTTTTTTCAGGTCGGCTTCTGCTTTTATGAACTTGCTGGTGGCCGGACTGAAAATTGTGGCGGAGTACTACCCAAGACGGCTCTTCAAAGCGTTCGTAATTGACCCTCCCACAATGTTCTCATATCTTTGGAAGGGTGTTCGACCGTTTGTTGAGCTATCAACGGCTACGATGATGGTATCATCTCTAGACTTTGAGGAGTCGTTGGATTTCAGTGACTTCTCAAATTACCCGCGAGCCTCGTCCCTCCGATTCGACCCTGCCTCAATCAAATCAACGGCCAAGGTCGGCTCTTGCTCATCCTCTCGTTTCTCCTTCACTGTCGCTCAGCATCAGTTCGACTCTCTCAAGCCTTGGTACCTCAGCCTCACAGACACGTCCGCATCCAAAGTAGGCCCCACCACACCCTCCCCGCTGGGACCCGCGCTCATCTCTCCGCTAAACGCCCGGTCCTTCTCCTTCGCATCCCCCGCCGCCAGAACACCACGTGGAACAATCCACGGCTATGGCAACTCCGCTGCCATGAGAAAAAGCCTGTTCCCGTCGACCCCACTCCCCCAACGGTGCTCCGGCAGCGAGCCCAGCAGAACCCCTCACCACAACCAGCCGCCGCGGACCCCACGCCCCTCGTTCCTCCAATCGCCGGCCACGTTCTTCCGCAGGGAGAGCCACGCCAGCAGGACGGAGAAGTCCCGGGAGTCCTTCGCGCCGTTCTTGAAGTTCTACAGGAGGCCGTACGACGAGATGATCTATCAGGTCAAAGATGCGGCCCCCACTGGGCGGACTCATCTCCATCGTCTCTCCCCACATCAGACGCCGCCACGTGTCCGTATCACAACGGTTCTGAACCCAGAGAAAGGGAAGAGAAGTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO

Protein Analysis

415

Amino Acids

46.47

Weight (kDa)

10.03

Isoelectric Point (pI)

52.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 95 - 179 4.5e-12 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012073)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05370 AT3G22410
fragaria_vesca FvH4_4g15420 FvH4_4g15420
malus_domestica MD13G1192100.v1.1 MD16G1192700.v1.1
prunus_persica Prupe.1G012800_v2.0.a1
pyrus_communis pycom13g16480 pycom16g16260
rosa_chinensis RchiOBHm_Chr4g0416361
rosa_laevigata RLG00000007989
rosa_multiflora Rmu_sc0001488.1_g000005
rosa_roxburghii Rroxscaffold_5G00360210
rosa_rugosa Rorug04G0141300
rosa_samantha Rh4AG203700 Rh4BG200900 Rh4CG214900 Rh4DG200600
rosa_wichuraiana Rw4G017200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1057
Acc65I GGTACC 1 cut(s) 726
AccB1I GGYRCC 1 cut(s) 726
AccI GTMKAC 1 cut(s) 914
AccII CGCG 4 cut(s) 604, 794, 982, 1076
AcoI YGGCCR 4 cut(s) 177, 411, 648, 1014
AcuI CTGAAG 2 cut(s) 53, 285
AcvI CACGTG 2 cut(s) 857, 1193
AcyI GRCGYC 1 cut(s) 1184
AdeI CACNNNGTG 1 cut(s) 857
AfaI GTAC 4 cut(s) 437, 728, 1106, 1240
AfiI CCNNNNNNNGG 4 cut(s) 1012, 1146, 1149, 1226
AflIII ACRYGT 3 cut(s) 279, 744, 1192
AgsI TTSAA 3 cut(s) 148, 457, 1087
AjiI CACGTC 1 cut(s) 747
AluBI AGCT 4 cut(s) 73, 254, 259, 526
AluI AGCT 4 cut(s) 73, 254, 259, 526
Alw21I GWGCWC 2 cut(s) 261, 938
Alw26I GTCTC 2 cut(s) 202, 1172
AlwNI CAGNNNCTG 1 cut(s) 76
Ama87I CYCGRG 2 cut(s) 22, 1062
AoxI GGCC 7 cut(s) 177, 411, 648, 763, 1014, 1100, 1137
ApeKI GCWGC 2 cut(s) 887, 942
Asp700I GAANNNNTTC 1 cut(s) 63
Asp718I GGTACC 1 cut(s) 726
AspLEI GCGC 2 cut(s) 796, 1078
AspS9I GGNCC 5 cut(s) 764, 788, 819, 984, 1138
AsuC2I CCSGG 3 cut(s) 817, 1063, 1064
AsuHPI GGTGA 2 cut(s) 8, 956
AvaI CYCGRG 2 cut(s) 22, 1062
AvaII GGWCC 3 cut(s) 788, 819, 984
BanI GGYRCC 1 cut(s) 726
BanII GRGCYC 2 cut(s) 261, 951
BbrPI CACGTG 2 cut(s) 857, 1193
BbsI GAAGAC 1 cut(s) 179
Bbv12I GWGCWC 2 cut(s) 261, 938
BbvCI CCTCAGC 1 cut(s) 731
BbvI GCAGC 2 cut(s) 874, 954
BccI CCATC 2 cut(s) 538, 1170
BceAI ACGGC 7 cut(s) 192, 464, 549, 663, 886, 1063, 1087
BcnI CCSGG 3 cut(s) 817, 1063, 1064
BcoDI GTCTC 2 cut(s) 202, 1172
BfaI CTAG 1 cut(s) 557
BfmI CTRYAG 1 cut(s) 1093
BisI GCNGC 7 cut(s) 843, 888, 943, 977, 980, 1137, 1187
BlpI GCTNAGC 2 cut(s) 255, 696
BlsI GCNGC 7 cut(s) 844, 889, 944, 978, 981, 1138, 1188
BmcAI AGTACT 1 cut(s) 437
Bme1390I CCNGG 3 cut(s) 817, 1063, 1064
Bme18I GGWCC 3 cut(s) 788, 819, 984
BmeT110I CYCGRG 2 cut(s) 22, 1062
BmgBI CACGTC 1 cut(s) 747
BmgT120I GGNCC 5 cut(s) 764, 788, 819, 984, 1138
BmiI GGNNCC 6 cut(s) 728, 766, 789, 790, 986, 1140
BmrFI CCNGG 3 cut(s) 817, 1063, 1064
BmrI ACTGGG 1 cut(s) 1155
BmsI GCATC 4 cut(s) 709, 761, 842, 1123
BmuI ACTGGG 1 cut(s) 1155
BpiI GAAGAC 1 cut(s) 179
BpmI CTGGAG 1 cut(s) 329
Bpu10I CCTNAGC 1 cut(s) 731
Bpu1102I GCTNAGC 2 cut(s) 255, 696
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 3 cut(s) 817, 1063, 1064
BsaAI YACGTR 3 cut(s) 280, 857, 1193
BsaHI GRCGYC 1 cut(s) 1184
BsaJI CCNNGG 5 cut(s) 651, 722, 868, 980, 1062
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 4 cut(s) 1012, 1146, 1149, 1226
Bse118I RCCGGY 1 cut(s) 1012
Bse1I ACTGG 1 cut(s) 1150
BseDI CCNNGG 5 cut(s) 651, 722, 868, 980, 1062
BseGI GGATG 3 cut(s) 668, 752, 833
BseLI CCNNNNNNNGG 4 cut(s) 1012, 1146, 1149, 1226
BseMII CTCAG 6 cut(s) 68, 216, 246, 264, 710, 745
BseNI ACTGG 1 cut(s) 1150
BseRI GAGGAG 1 cut(s) 581
BseXI GCAGC 2 cut(s) 874, 954
BseYI CCCAGC 2 cut(s) 784, 950
Bsh1236I CGCG 4 cut(s) 604, 794, 982, 1076
Bsh1285I CGRYCG 1 cut(s) 515
BshFI GGCC 7 cut(s) 179, 413, 650, 765, 1016, 1102, 1139
BshNI GGYRCC 1 cut(s) 726
BsiEI CGRYCG 1 cut(s) 515
BsiHKAI GWGCWC 2 cut(s) 261, 938
BsiHKCI CYCGRG 2 cut(s) 22, 1062
BsiSI CCGG 5 cut(s) 414, 817, 939, 1013, 1063
BsiWI CGTACG 1 cut(s) 1104
BslFI GGGAC 3 cut(s) 598, 801, 1045
BslI CCNNNNNNNGG 4 cut(s) 1012, 1146, 1149, 1226
BsmAI GTCTC 2 cut(s) 202, 1172
BsmBI CGTCTC 1 cut(s) 1172
BsmFI GGGAC 3 cut(s) 598, 801, 1045
BsnI GGCC 7 cut(s) 179, 413, 650, 765, 1016, 1102, 1139
BsoBI CYCGRG 2 cut(s) 22, 1062
Bsp1286I GDGCHC 3 cut(s) 261, 938, 951
Bsp143I GATC 2 cut(s) 13, 1116
Bsp1720I GCTNAGC 2 cut(s) 255, 696
BspANI GGCC 7 cut(s) 179, 413, 650, 765, 1016, 1102, 1139
BspCNI CTCAG 6 cut(s) 69, 217, 247, 263, 709, 744
BspFNI CGCG 4 cut(s) 604, 794, 982, 1076
BspLI GGNNCC 6 cut(s) 728, 766, 789, 790, 986, 1140
BspQI GCTCTTC 1 cut(s) 458
BspT107I GGYRCC 1 cut(s) 726
BsrFI RCCGGY 1 cut(s) 1012
BsrI ACTGG 1 cut(s) 1150
BssAI RCCGGY 1 cut(s) 1012
BssECI CCNNGG 5 cut(s) 651, 722, 868, 980, 1062
BssMI GATC 2 cut(s) 13, 1116
BssNI GRCGYC 1 cut(s) 1184
BssT1I CCWWGG 2 cut(s) 651, 722
Bst4CI ACNGT 5 cut(s) 100, 516, 691, 933, 1210
Bst6I CTCTTC 2 cut(s) 458, 1226
BstACI GRCGYC 1 cut(s) 1184
BstBAI YACGTR 3 cut(s) 280, 857, 1193
BstC8I GCNNGC 6 cut(s) 196, 261, 606, 947, 1014, 1045
BstDEI CTNAG 7 cut(s) 77, 188, 225, 250, 255, 696, 731
BstDSI CCRYGG 2 cut(s) 868, 980
BstF5I GGATG 3 cut(s) 668, 752, 833
BstFNI CGCG 4 cut(s) 604, 794, 982, 1076
BstHHI GCGC 2 cut(s) 796, 1078
BstKTI GATC 2 cut(s) 16, 1119
BstMAI GTCTC 2 cut(s) 202, 1172
BstMBI GATC 2 cut(s) 13, 1116
BstMCI CGRYCG 1 cut(s) 515
BstMWI GCNNNNNNNGC 6 cut(s) 260, 839, 942, 951, 1035, 1044
BstSCI CCNGG 3 cut(s) 815, 1061, 1062
BstSFI CTRYAG 1 cut(s) 1093
BstUI CGCG 4 cut(s) 604, 794, 982, 1076
BstV1I GCAGC 2 cut(s) 874, 954
BstV2I GAAGAC 1 cut(s) 179
BstXI CCANNNNNNTGG 1 cut(s) 875
BsuRI GGCC 7 cut(s) 179, 413, 650, 765, 1016, 1102, 1139
BtgI CCRYGG 2 cut(s) 868, 980
BtrI CACGTC 1 cut(s) 747
BtsCI GGATG 3 cut(s) 668, 752, 833
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 5 cut(s) 123, 222, 589, 687, 1143
Cac8I GCNNGC 6 cut(s) 196, 261, 606, 947, 1014, 1045
CaiI CAGNNNCTG 1 cut(s) 76
CfoI GCGC 2 cut(s) 796, 1078
Cfr10I RCCGGY 1 cut(s) 1012
Cfr13I GGNCC 5 cut(s) 764, 788, 819, 984, 1138
Cfr42I CCGCGG 1 cut(s) 983
Cfr9I CCCGGG 1 cut(s) 1062
CseI GACGC 2 cut(s) 374, 1192
Csp6I GTAC 4 cut(s) 436, 727, 1105, 1239
CviAII CATG 2 cut(s) 328, 892
CviQI GTAC 4 cut(s) 436, 727, 1105, 1239
DdeI CTNAG 7 cut(s) 77, 188, 225, 250, 255, 696, 731
DpnI GATC 2 cut(s) 15, 1118
DpnII GATC 2 cut(s) 13, 1116
DraIII CACNNNGTG 1 cut(s) 857
DrdI GACNNNNNNGTC 1 cut(s) 1057
DseDI GACNNNNNNGTC 1 cut(s) 1057
EaeI YGGCCR 4 cut(s) 177, 411, 648, 1014
Eam1104I CTCTTC 2 cut(s) 458, 1226
EarI CTCTTC 2 cut(s) 458, 1226
EciI GGCGGA 2 cut(s) 446, 1165
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 2 cut(s) 651, 722
Eco24I GRGCYC 2 cut(s) 261, 951
Eco47I GGWCC 3 cut(s) 788, 819, 984
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 53, 285
Eco72I CACGTG 2 cut(s) 857, 1193
Eco88I CYCGRG 2 cut(s) 22, 1062
EcoICRI GAGCTC 1 cut(s) 259
EcoO109I RGGNCCY 2 cut(s) 764, 788
EcoT14I CCWWGG 2 cut(s) 651, 722
EcoT38I GRGCYC 2 cut(s) 261, 951
ErhI CCWWGG 2 cut(s) 651, 722
Esp3I CGTCTC 1 cut(s) 1172
FaeI CATG 2 cut(s) 331, 895
FaiI YATR 6 cut(s) 329, 398, 493, 876, 893, 1243
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 3 cut(s) 598, 801, 1045
FatI CATG 2 cut(s) 327, 891
FauI CCCGC 4 cut(s) 609, 789, 799, 847
FblI GTMKAC 1 cut(s) 914
Fnu4HI GCNGC 7 cut(s) 843, 888, 943, 977, 980, 1137, 1187
FokI GGATG 3 cut(s) 655, 739, 820
FriOI GRGCYC 2 cut(s) 261, 951
Fsp4HI GCNGC 7 cut(s) 843, 888, 943, 977, 980, 1137, 1187
FspBI CTAG 1 cut(s) 557
GlaI GCGC 2 cut(s) 795, 1077
GluI GCNGC 7 cut(s) 843, 888, 943, 977, 980, 1137, 1187
GsaI CCCAGC 2 cut(s) 788, 954
GsuI CTGGAG 1 cut(s) 329
HaeIII GGCC 7 cut(s) 179, 413, 650, 765, 1016, 1102, 1139
HapII CCGG 5 cut(s) 414, 817, 939, 1013, 1063
HgaI GACGC 2 cut(s) 374, 1192
HhaI GCGC 2 cut(s) 796, 1078
Hin1I GRCGYC 1 cut(s) 1184
Hin1II CATG 2 cut(s) 331, 895
Hin6I GCGC 2 cut(s) 794, 1076
HinP1I GCGC 2 cut(s) 794, 1076
HincII GTYRAC 1 cut(s) 915
HindII GTYRAC 1 cut(s) 915
HinfI GANTC 6 cut(s) 319, 569, 621, 710, 1067, 1153
HpaII CCGG 5 cut(s) 414, 817, 939, 1013, 1063
HphI GGTGA 2 cut(s) 8, 956
Hpy166II GTNNAC 2 cut(s) 285, 915
Hpy188I TCNGA 4 cut(s) 78, 620, 1182, 1215
Hpy188III TCNNGA 2 cut(s) 557, 1084
Hpy8I GTNNAC 2 cut(s) 285, 915
Hpy99I CGWCG 3 cut(s) 368, 916, 1112
HpyAV CCTTC 6 cut(s) 260, 496, 694, 832, 838, 1081
HpyCH4III ACNGT 5 cut(s) 100, 516, 691, 933, 1210
HpyCH4IV ACGT 6 cut(s) 279, 339, 746, 856, 1019, 1192
HpyCH4V TGCA 2 cut(s) 50, 120
HpyF10VI GCNNNNNNNGC 6 cut(s) 260, 839, 942, 951, 1035, 1044
HpyF3I CTNAG 7 cut(s) 77, 188, 225, 250, 255, 696, 731
HpySE526I ACGT 6 cut(s) 279, 339, 746, 856, 1019, 1192
Hsp92I GRCGYC 1 cut(s) 1184
Hsp92II CATG 2 cut(s) 331, 895
HspAI GCGC 2 cut(s) 794, 1076
KflI GGGWCCC 1 cut(s) 788
KpnI GGTACC 1 cut(s) 730
KroI GCCGGC 1 cut(s) 1012
KroNI GCCGGC 1 cut(s) 1014
KspI CCGCGG 1 cut(s) 983
Kzo9I GATC 2 cut(s) 13, 1116
LguI GCTCTTC 1 cut(s) 458
LmnI GCTCC 1 cut(s) 941
Lsp1109I GCAGC 2 cut(s) 874, 954
LweI GCATC 4 cut(s) 709, 761, 842, 1123
MaeI CTAG 1 cut(s) 557
MaeII ACGT 6 cut(s) 279, 339, 746, 856, 1019, 1192
MaeIII GTNAC 1 cut(s) 584
MalI GATC 2 cut(s) 15, 1118
MboI GATC 2 cut(s) 13, 1116
MboII GAAGA 6 cut(s) 19, 59, 184, 445, 1015, 1243
MhlI GDGCHC 3 cut(s) 261, 938, 951
MluCI AATT 3 cut(s) 423, 468, 595
MlyI GAGTC 4 cut(s) 578, 704, 1076, 1147
MmeI TCCRAC 1 cut(s) 555
MroNI GCCGGC 1 cut(s) 1012
MroXI GAANNNNTTC 1 cut(s) 63
MseI TTAA 2 cut(s) 102, 168
MslI CAYNNNNRTG 1 cut(s) 873
MspA1I CMGCKG 4 cut(s) 254, 784, 887, 982
MspI CCGG 5 cut(s) 414, 817, 939, 1013, 1063
MspR9I CCNGG 3 cut(s) 817, 1063, 1064
MvnI CGCG 4 cut(s) 604, 794, 982, 1076
MwoI GCNNNNNNNGC 6 cut(s) 260, 839, 942, 951, 1035, 1044
NaeI GCCGGC 1 cut(s) 1014
NciI CCSGG 3 cut(s) 817, 1063, 1064
NdeII GATC 2 cut(s) 13, 1116
NgoMIV GCCGGC 1 cut(s) 1012
NlaIII CATG 2 cut(s) 331, 895
NlaIV GGNNCC 6 cut(s) 728, 766, 789, 790, 986, 1140
NmuCI GTSAC 1 cut(s) 584
PciSI GCTCTTC 1 cut(s) 458
PcsI WCGNNNNNNNCGW 1 cut(s) 1016
PdiI GCCGGC 1 cut(s) 1014
PdmI GAANNNNTTC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 319, 621
Pfl23II CGTACG 1 cut(s) 1104
PkrI GCNGC 7 cut(s) 844, 889, 944, 978, 981, 1138, 1188
PleI GAGTC 4 cut(s) 577, 704, 1075, 1147
PmaCI CACGTG 2 cut(s) 857, 1193
PmlI CACGTG 2 cut(s) 857, 1193
PpsI GAGTC 4 cut(s) 577, 704, 1075, 1147
Ppu21I YACGTR 3 cut(s) 280, 857, 1193
PpuMI RGGWCCY 1 cut(s) 788
Psp124BI GAGCTC 1 cut(s) 261
Psp5II RGGWCCY 1 cut(s) 788
PspCI CACGTG 2 cut(s) 857, 1193
PspFI CCCAGC 2 cut(s) 784, 950
PspLI CGTACG 1 cut(s) 1104
PspN4I GGNNCC 6 cut(s) 728, 766, 789, 790, 986, 1140
PspPI GGNCC 5 cut(s) 764, 788, 819, 984, 1138
PspPPI RGGWCCY 1 cut(s) 788
PstNI CAGNNNCTG 1 cut(s) 76
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 4 cut(s) 437, 728, 1106, 1240
RsaNI GTAC 4 cut(s) 436, 727, 1105, 1239
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 1 cut(s) 261
SacII CCGCGG 1 cut(s) 983
SalI GTCGAC 1 cut(s) 913
SapI GCTCTTC 1 cut(s) 458
SaqAI TTAA 2 cut(s) 102, 168
SatI GCNGC 7 cut(s) 843, 888, 943, 977, 980, 1137, 1187
Sau3AI GATC 2 cut(s) 13, 1116
Sau96I GGNCC 5 cut(s) 764, 788, 819, 984, 1138
ScaI AGTACT 1 cut(s) 437
SchI GAGTC 4 cut(s) 578, 704, 1076, 1147
ScrFI CCNGG 3 cut(s) 817, 1063, 1064
SduI GDGCHC 3 cut(s) 261, 938, 951
SfaNI GCATC 4 cut(s) 709, 761, 842, 1123
SfcI CTRYAG 1 cut(s) 1093
Sfr303I CCGCGG 1 cut(s) 983
SgrBI CCGCGG 1 cut(s) 983
SinI GGWCC 3 cut(s) 788, 819, 984
SmaI CCCGGG 1 cut(s) 1064
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 1 cut(s) 716
SmoI CTYRAG 1 cut(s) 716
Sse9I AATT 3 cut(s) 423, 468, 595
SspI AATATT 1 cut(s) 217
SspMI CTAG 1 cut(s) 557
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 815, 1061, 1062
StyI CCWWGG 2 cut(s) 651, 722
TaaI ACNGT 5 cut(s) 100, 516, 691, 933, 1210
TaiI ACGT 6 cut(s) 282, 342, 749, 859, 1022, 1195
TaqI TCGA 5 cut(s) 363, 511, 624, 708, 914
TasI AATT 3 cut(s) 423, 468, 595
TatI WGTACW 2 cut(s) 435, 1238
TauI GCSGC 5 cut(s) 845, 979, 982, 1139, 1189
TfiI GAWTC 2 cut(s) 319, 621
Tru1I TTAA 2 cut(s) 102, 168
Tru9I TTAA 2 cut(s) 102, 168
TscAI CASTG 5 cut(s) 130, 229, 589, 694, 1150
TseFI GTSAC 1 cut(s) 584
TseI GCWGC 2 cut(s) 887, 942
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 2 cut(s) 45, 413
TspGWI ACGGA 2 cut(s) 1067, 1187
TspMI CCCGGG 1 cut(s) 1062
TspRI CASTG 5 cut(s) 130, 229, 589, 694, 1150
VpaK11BI GGWCC 3 cut(s) 788, 819, 984
XbaI TCTAGA 1 cut(s) 556
XmaI CCCGGG 1 cut(s) 1062
XmiI GTMKAC 1 cut(s) 914
XmnI GAANNNNTTC 1 cut(s) 63
XspI CTAG 1 cut(s) 557
ZrmI AGTACT 1 cut(s) 437
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.