pycom13g16640

Lactoylglutathione lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012372
Physical Location & Seq
Reverse (-)
12413461 .. 12415463
2003 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGTTAGTCCGTAATCACAATTCACAAAGCAATGACGTTTCACGACGTCGTATTCACATCACCCTTAGAAGCCTAGCCAATAAAATCAGCCGTTATATAAGGTCCCCCAAACCGCTTGCCAAAAGAAAGAAGAAAGAAATGGCGGCAATAGCAACAGGAGTGAGTCTCAACCACATTTCCAGAGAATCCTCCGACGTTCGGCGTCTCGCCGATTTCTACAAAGAGATATTTGGGCTCGAGGAGATTGAGAGCCCAAACTTCGGGGAGTTCAAGGTGATATGGCTCAAGGCGCCTGGGAATTTCGCTCTCCACCTGATCGAAAGAAACCCTAGTTTTAACCTTCCGGAAGGACCATGGAGCGCCACTTCACCGGTTGCTGACCCGAGCCACCTCCCCAGAGGCCACCACATCTGCTTCAACGTCTCCAATTTCCAGTCTTTTGCGAAAACCCTCAAGGAAAGGGGAATCCAGACGTTTGAGAAGTCCTTACCTGATGGTAAGGTCAAGCAAGTCTTCTTCTTTGATCCAGATGGCAATGGATTGGAGGTTGCAGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyoxalase Glyoxalase_4

Protein Analysis

187

Amino Acids

21.07

Weight (kDa)

9.89

Isoelectric Point (pI)

65.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxalase PF00903 56 - 183 1.9e-09 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Glyoxalase_4 PF13669 58 - 167 1.2e-06 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012372)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32090 AT2G32090
fragaria_vesca FvH4_4g16340 FvH4_4g16340
malus_domestica MD13G1194100.v1.1
prunus_persica Prupe.1G014900_v2.0.a1 Prupe.1G014900_v2.0.a1
pyrus_communis pycom13g16640
rosa_chinensis RchiOBHm_Chr4g0416051
rosa_laevigata RLG00000008022
rosa_multiflora Rmu_sc0007573.1_g000005 Rmu_sc0007573.1_g000007
rosa_roxburghii Rroxscaffold_5G00359880
rosa_rugosa Rorug04G0137700
rosa_samantha Rh4AG200000 Rh4BG197600 Rh4CG211300 Rh4DG197800
rosa_wichuraiana Rw4G016980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 49
Acc36I ACCTGC 1 cut(s) 542
AccB1I GGYRCC 1 cut(s) 289
AccIII TCCGGA 1 cut(s) 343
AciI CCGC 2 cut(s) 113, 143
AclWI GGATC 1 cut(s) 518
AcsI RAATTY 1 cut(s) 298
AcyI GRCGYC 3 cut(s) 46, 202, 290
AfiI CCNNNNNNNGG 2 cut(s) 198, 260
AgeI ACCGGT 1 cut(s) 370
AgsI TTSAA 2 cut(s) 271, 418
AjnI CCWGG 1 cut(s) 292
Alw26I GTCTC 3 cut(s) 170, 209, 427
AlwI GGATC 1 cut(s) 518
Ama87I CYCGRG 2 cut(s) 236, 382
Aor13HI TCCGGA 1 cut(s) 343
AoxI GGCC 1 cut(s) 400
ApoI RAATTY 1 cut(s) 298
AsiGI ACCGGT 1 cut(s) 370
AspLEI GCGC 2 cut(s) 292, 362
AspS9I GGNCC 2 cut(s) 102, 350
AsuHPI GGTGA 3 cut(s) 52, 286, 360
AvaI CYCGRG 2 cut(s) 236, 382
AvaII GGWCC 2 cut(s) 102, 350
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 2 cut(s) 237, 254
BbsI GAAGAC 1 cut(s) 505
BccI CCATC 2 cut(s) 488, 524
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 294
BcoDI GTCTC 3 cut(s) 170, 209, 427
BfaI CTAG 2 cut(s) 74, 330
BfoI RGCGCY 2 cut(s) 293, 363
BfuAI ACCTGC 1 cut(s) 542
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme1390I CCNGG 1 cut(s) 294
Bme18I GGWCC 2 cut(s) 102, 350
BmeT110I CYCGRG 2 cut(s) 236, 382
BmgT120I GGNCC 2 cut(s) 102, 350
BmiI GGNNCC 2 cut(s) 104, 291
BmrFI CCNGG 1 cut(s) 294
BpiI GAAGAC 1 cut(s) 505
BplI GAGNNNNNCTC 2 cut(s) 150, 182
BpuEI CTTGAG 2 cut(s) 269, 437
BsaHI GRCGYC 3 cut(s) 46, 202, 290
BsaJI CCNNGG 2 cut(s) 293, 353
BsaWI WCCGGW 2 cut(s) 343, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 260
Bse118I RCCGGY 1 cut(s) 370
Bse1I ACTGG 1 cut(s) 433
Bse3DI GCAATG 2 cut(s) 37, 541
BseAI TCCGGA 1 cut(s) 343
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 293, 353
BseLI CCNNNNNNNGG 2 cut(s) 198, 260
BseMI GCAATG 2 cut(s) 37, 541
BseNI ACTGG 1 cut(s) 433
BseRI GAGGAG 1 cut(s) 254
BshFI GGCC 1 cut(s) 402
BshNI GGYRCC 1 cut(s) 289
BshTI ACCGGT 1 cut(s) 370
BsiHKCI CYCGRG 2 cut(s) 236, 382
BsiSI CCGG 2 cut(s) 344, 371
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 2 cut(s) 198, 260
BsmAI GTCTC 3 cut(s) 170, 209, 427
BsmBI CGTCTC 2 cut(s) 209, 427
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 1 cut(s) 402
BsoBI CYCGRG 2 cut(s) 236, 382
Bsp1286I GDGCHC 2 cut(s) 237, 254
Bsp13I TCCGGA 1 cut(s) 343
Bsp143I GATC 2 cut(s) 315, 523
Bsp19I CCATGG 1 cut(s) 353
BspACI CCGC 2 cut(s) 113, 143
BspANI GGCC 1 cut(s) 402
BspEI TCCGGA 1 cut(s) 343
BspLI GGNNCC 2 cut(s) 104, 291
BspMI ACCTGC 1 cut(s) 542
BspPI GGATC 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 289
BsrDI GCAATG 2 cut(s) 37, 541
BsrFI RCCGGY 1 cut(s) 370
BsrI ACTGG 1 cut(s) 433
BssAI RCCGGY 1 cut(s) 370
BssECI CCNNGG 2 cut(s) 293, 353
BssMI GATC 2 cut(s) 315, 523
BssNI GRCGYC 3 cut(s) 46, 202, 290
BssT1I CCWWGG 1 cut(s) 353
Bst2UI CCWGG 1 cut(s) 294
BstACI GRCGYC 3 cut(s) 46, 202, 290
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 1 cut(s) 65
BstDSI CCRYGG 1 cut(s) 353
BstH2I RGCGCY 2 cut(s) 293, 363
BstHHI GCGC 2 cut(s) 292, 362
BstKTI GATC 2 cut(s) 318, 526
BstMAI GTCTC 3 cut(s) 170, 209, 427
BstMBI GATC 2 cut(s) 315, 523
BstMWI GCNNNNNNNGC 2 cut(s) 149, 289
BstNI CCWGG 1 cut(s) 294
BstSCI CCNGG 1 cut(s) 292
BstV2I GAAGAC 1 cut(s) 505
BsuRI GGCC 1 cut(s) 402
BtgI CCRYGG 1 cut(s) 353
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 1 cut(s) 117
CfoI GCGC 2 cut(s) 292, 362
Cfr10I RCCGGY 1 cut(s) 370
Cfr13I GGNCC 2 cut(s) 102, 350
CseI GACGC 1 cut(s) 191
CspAI ACCGGT 1 cut(s) 370
CviAII CATG 1 cut(s) 354
CviJI RGCY 8 cut(s) 72, 77, 90, 235, 252, 283, 387, 402
CviKI_1 RGCY 8 cut(s) 72, 77, 90, 235, 252, 283, 387, 402
DdeI CTNAG 1 cut(s) 65
DinI GGCGCC 1 cut(s) 291
DpnI GATC 2 cut(s) 317, 525
DpnII GATC 2 cut(s) 315, 523
Eco130I CCWWGG 1 cut(s) 353
Eco24I GRGCYC 2 cut(s) 237, 254
Eco47I GGWCC 2 cut(s) 102, 350
Eco88I CYCGRG 2 cut(s) 236, 382
EcoO109I RGGNCCY 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 353
EcoT38I GRGCYC 2 cut(s) 237, 254
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 353
Esp3I CGTCTC 2 cut(s) 209, 427
FaeI CATG 1 cut(s) 357
FaiI YATR 4 cut(s) 96, 98, 280, 355
FalI AAGNNNNNCTT 2 cut(s) 497, 529
FaqI GGGAC 1 cut(s) 88
FatI CATG 1 cut(s) 353
Fnu4HI GCNGC 1 cut(s) 144
FriOI GRGCYC 2 cut(s) 237, 254
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 2 cut(s) 74, 330
GlaI GCGC 2 cut(s) 291, 361
GluI GCNGC 1 cut(s) 144
HaeII RGCGCY 2 cut(s) 293, 363
HaeIII GGCC 1 cut(s) 402
HapII CCGG 2 cut(s) 344, 371
HgaI GACGC 1 cut(s) 191
HhaI GCGC 2 cut(s) 292, 362
Hin1I GRCGYC 3 cut(s) 46, 202, 290
Hin1II CATG 1 cut(s) 357
Hin6I GCGC 2 cut(s) 290, 360
HinP1I GCGC 2 cut(s) 290, 360
HinfI GANTC 3 cut(s) 163, 185, 465
HpaII CCGG 2 cut(s) 344, 371
HphI GGTGA 3 cut(s) 52, 286, 360
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 5 cut(s) 42, 180, 344, 469, 527
Hpy99I CGWCG 3 cut(s) 48, 51, 197
HpyAV CCTTC 2 cut(s) 341, 350
HpyCH4IV ACGT 5 cut(s) 36, 46, 195, 420, 473
HpyCH4V TGCA 1 cut(s) 551
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 289
HpyF3I CTNAG 1 cut(s) 65
HpySE526I ACGT 5 cut(s) 36, 46, 195, 420, 473
Hsp92I GRCGYC 3 cut(s) 46, 202, 290
Hsp92II CATG 1 cut(s) 357
HspAI GCGC 2 cut(s) 290, 360
KasI GGCGCC 1 cut(s) 289
Kpn2I TCCGGA 1 cut(s) 343
Kzo9I GATC 2 cut(s) 315, 523
LmnI GCTCC 1 cut(s) 357
MaeI CTAG 2 cut(s) 74, 330
MaeII ACGT 5 cut(s) 36, 46, 195, 420, 473
MalI GATC 2 cut(s) 317, 525
MboI GATC 2 cut(s) 315, 523
MboII GAAGA 3 cut(s) 142, 505, 508
MhlI GDGCHC 2 cut(s) 237, 254
MluCI AATT 3 cut(s) 19, 298, 427
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 1 cut(s) 172
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 6 cut(s) 199, 232, 392, 401, 461, 538
MroI TCCGGA 1 cut(s) 343
MseI TTAA 1 cut(s) 336
MspI CCGG 2 cut(s) 344, 371
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 149, 289
NarI GGCGCC 1 cut(s) 290
NcoI CCATGG 1 cut(s) 353
NdeII GATC 2 cut(s) 315, 523
NlaIII CATG 1 cut(s) 357
NlaIV GGNNCC 2 cut(s) 104, 291
PaeR7I CTCGAG 1 cut(s) 236
PfeI GAWTC 2 cut(s) 185, 465
PinAI ACCGGT 1 cut(s) 370
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 171
PluTI GGCGCC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 171
PpuMI RGGWCCY 1 cut(s) 102
Psp5II RGGWCCY 1 cut(s) 102
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspN4I GGNNCC 2 cut(s) 104, 291
PspPI GGNCC 2 cut(s) 102, 350
PspPPI RGGWCCY 1 cut(s) 102
PspXI VCTCGAGB 1 cut(s) 236
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 2 cut(s) 315, 523
Sau96I GGNCC 2 cut(s) 102, 350
SchI GAGTC 1 cut(s) 172
ScrFI CCNGG 1 cut(s) 294
SduI GDGCHC 2 cut(s) 237, 254
SfoI GGCGCC 1 cut(s) 291
Sfr274I CTCGAG 1 cut(s) 236
SinI GGWCC 2 cut(s) 102, 350
SlaI CTCGAG 1 cut(s) 236
SmlI CTYRAG 3 cut(s) 236, 284, 452
SmoI CTYRAG 3 cut(s) 236, 284, 452
Sse9I AATT 3 cut(s) 19, 298, 427
SsiI CCGC 2 cut(s) 113, 143
SspDI GGCGCC 1 cut(s) 289
SspMI CTAG 2 cut(s) 74, 330
StyD4I CCNGG 1 cut(s) 292
StyI CCWWGG 1 cut(s) 353
TaiI ACGT 5 cut(s) 39, 49, 198, 423, 476
TaqI TCGA 2 cut(s) 237, 318
TasI AATT 3 cut(s) 19, 298, 427
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 2 cut(s) 185, 465
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
VpaK11BI GGWCC 2 cut(s) 102, 350
XapI RAATTY 1 cut(s) 298
XhoI CTCGAG 1 cut(s) 236
XspI CTAG 2 cut(s) 74, 330
ZraI GACGTC 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.