pycom13g23700

At4g14100-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0015595
Physical Location & Seq
Forward (+)
21125850 .. 21126681
832 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 567 bp
ATGCAGGAGCTCAAACTACCACTGCAACTATGCTTAACAACCTGCATAATACTGCAGGTACTGACTTGCTCAAACTCGAACCCAACACCGTCGCCATGGCCACTGCAATTCCATGCACTTCTATTCATGAACTTGACCACAACACACCTCCAGCTCAGCGACCTCTGGTACGACTGGCCGAGAGGTAGGAACGTCAACATATTTCAGAAGCAGCTGGGTGAGGTTTTGTACGACATAGAGTGGAACAACGGCACATCATTTTACTACTCCGTCGGTGAGAACGGAAGTTGTGAGGTTATGGGGTTTGAAGTCGGCATTCCGAGGCCCGATTTTCTTGAAGGAGCAGAGTATCTAGGGACGGAAGTCACAGATGGGTTTCTTTGCAATGTGTGGGAGAAGGTGGATTTTATTTGGTACTATGAGGATGTTTACACCAAGAGGCCAGTTCGTTGGGATTTCTATGATGGAATATCTTCTCAGGTAATGACATTTGAGGTTGGTGCAGTGTTACCAGATACAGTCACTCAAGCACCTGCTTACTGCTTCAACCAAGACAAAGATGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

189

Amino Acids

21.69

Weight (kDa)

4.26

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08920 FvH4_4g08920 FvH4_4g08920
malus_domestica MD13G1264800.v1.1
prunus_persica Prupe.1G092000_v2.0.a1
pyrus_communis pycom13g23700
rosa_chinensis RchiOBHm_Chr4g0403651
rosa_laevigata RLG00000008962
rosa_multiflora Rmu_co8223106.1_g000001
rosa_roxburghii Rroxscaffold_5G00348250
rosa_rugosa Rorug04G0044800
rosa_samantha Rh4AG119800 Rh4BG113100 Rh4CG128000 Rh4DG112500
rosa_wichuraiana Rw4G009730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 541
Acc36I ACCTGC 3 cut(s) 46, 50, 541
AcoI YGGCCR 2 cut(s) 98, 176
AfaI GTAC 4 cut(s) 60, 170, 230, 416
AgsI TTSAA 3 cut(s) 308, 338, 547
AluBI AGCT 3 cut(s) 10, 154, 214
AluI AGCT 3 cut(s) 10, 154, 214
Alw21I GWGCWC 1 cut(s) 12
AlwNI CAGNNNCTG 1 cut(s) 61
AoxI GGCC 4 cut(s) 98, 176, 323, 440
ApeKI GCWGC 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 472
AspS9I GGNCC 1 cut(s) 324
AsuHPI GGTGA 2 cut(s) 230, 287
BalI TGGCCA 1 cut(s) 100
BanII GRGCYC 1 cut(s) 12
Bbv12I GWGCWC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 223
BccI CCATC 2 cut(s) 365, 458
BceAI ACGGC 1 cut(s) 265
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 1 cut(s) 53
BfuAI ACCTGC 3 cut(s) 46, 50, 541
BisI GCNGC 1 cut(s) 212
BlpI GCTNAGC 1 cut(s) 155
BlsI GCNGC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 324
BmsI GCATC 1 cut(s) 550
BoxI GACNNNNGTC 1 cut(s) 362
BpmI CTGGAG 1 cut(s) 134
Bpu1102I GCTNAGC 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 510
BsaJI CCNNGG 2 cut(s) 95, 320
Bse1I ACTGG 2 cut(s) 179, 443
Bse3DI GCAATG 1 cut(s) 391
BseDI CCNNGG 2 cut(s) 95, 320
BseGI GGATG 1 cut(s) 430
BseMI GCAATG 1 cut(s) 391
BseMII CTCAG 2 cut(s) 169, 491
BseNI ACTGG 2 cut(s) 179, 443
BseXI GCAGC 1 cut(s) 223
BseYI CCCAGC 1 cut(s) 214
BsgI GTGCAG 1 cut(s) 522
BshFI GGCC 4 cut(s) 100, 178, 325, 442
BsiHKAI GWGCWC 1 cut(s) 12
BslFI GGGAC 1 cut(s) 370
BsmFI GGGAC 1 cut(s) 370
BsmI GAATGC 1 cut(s) 315
BsnI GGCC 4 cut(s) 100, 178, 325, 442
Bsp1286I GDGCHC 1 cut(s) 12
Bsp1720I GCTNAGC 1 cut(s) 155
Bsp19I CCATGG 1 cut(s) 95
BspANI GGCC 4 cut(s) 100, 178, 325, 442
BspCNI CTCAG 2 cut(s) 168, 490
BspHI TCATGA 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 57
BspMI ACCTGC 3 cut(s) 46, 50, 541
BsrDI GCAATG 1 cut(s) 391
BsrI ACTGG 2 cut(s) 179, 443
BssECI CCNNGG 2 cut(s) 95, 320
BssT1I CCWWGG 1 cut(s) 95
Bst4CI ACNGT 2 cut(s) 90, 520
BstDEI CTNAG 2 cut(s) 155, 477
BstDSI CCRYGG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 430
BstPAI GACNNNNGTC 1 cut(s) 362
BstSFI CTRYAG 1 cut(s) 53
BstV1I GCAGC 1 cut(s) 223
BstXI CCANNNNNNTGG 1 cut(s) 450
BsuRI GGCC 4 cut(s) 100, 178, 325, 442
BtgI CCRYGG 1 cut(s) 95
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 3 cut(s) 20, 101, 510
BtsIMutI CAGTG 3 cut(s) 20, 101, 510
BveI ACCTGC 3 cut(s) 46, 50, 541
CaiI CAGNNNCTG 1 cut(s) 61
CciI TCATGA 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 324
Csp6I GTAC 4 cut(s) 59, 169, 229, 415
CviAII CATG 3 cut(s) 96, 113, 127
CviJI RGCY 7 cut(s) 10, 100, 154, 178, 214, 325, 442
CviKI_1 RGCY 7 cut(s) 10, 100, 154, 178, 214, 325, 442
CviQI GTAC 4 cut(s) 59, 169, 229, 415
DdeI CTNAG 2 cut(s) 155, 477
EaeI YGGCCR 2 cut(s) 98, 176
Ecl136II GAGCTC 1 cut(s) 10
Eco130I CCWWGG 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 12
Eco53kI GAGCTC 1 cut(s) 10
EcoICRI GAGCTC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 95
EcoT22I ATGCAT 1 cut(s) 565
EcoT38I GRGCYC 1 cut(s) 12
ErhI CCWWGG 1 cut(s) 95
FaeI CATG 3 cut(s) 99, 116, 130
FaqI GGGAC 1 cut(s) 370
FatI CATG 3 cut(s) 95, 112, 126
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 12
Fsp4HI GCNGC 1 cut(s) 212
FspBI CTAG 1 cut(s) 353
GluI GCNGC 1 cut(s) 212
GsaI CCCAGC 1 cut(s) 218
GsuI CTGGAG 1 cut(s) 134
HaeIII GGCC 4 cut(s) 100, 178, 325, 442
Hin1II CATG 3 cut(s) 99, 116, 130
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HphI GGTGA 2 cut(s) 230, 287
Hpy166II GTNNAC 2 cut(s) 196, 430
Hpy188I TCNGA 2 cut(s) 207, 321
Hpy188III TCNNGA 2 cut(s) 127, 335
Hpy8I GTNNAC 2 cut(s) 196, 430
Hpy99I CGWCG 2 cut(s) 94, 275
HpyAV CCTTC 2 cut(s) 332, 391
HpyCH4III ACNGT 2 cut(s) 90, 520
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 9 cut(s) 4, 25, 45, 55, 106, 116, 384, 503, 563
HpyF3I CTNAG 2 cut(s) 155, 477
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 3 cut(s) 99, 116, 130
LmnI GCTCC 2 cut(s) 7, 341
Lsp1109I GCAGC 1 cut(s) 223
LweI GCATC 1 cut(s) 550
MaeI CTAG 1 cut(s) 353
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 3 cut(s) 364, 507, 520
MboII GAAGA 1 cut(s) 465
MhlI GDGCHC 1 cut(s) 12
MlsI TGGCCA 1 cut(s) 100
MluCI AATT 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 100
MnlI CCTC 9 cut(s) 158, 173, 176, 214, 286, 315, 415, 432, 487
Mox20I TGGCCA 1 cut(s) 100
Mph1103I ATGCAT 1 cut(s) 565
MroXI GAANNNNTTC 1 cut(s) 472
MscI TGGCCA 1 cut(s) 100
MseI TTAA 1 cut(s) 35
Msp20I TGGCCA 1 cut(s) 100
MspA1I CMGCKG 1 cut(s) 214
Mva1269I GAATGC 1 cut(s) 315
NcoI CCATGG 1 cut(s) 95
NlaIII CATG 3 cut(s) 99, 116, 130
NmeAIII GCCGAG 1 cut(s) 204
NmuCI GTSAC 2 cut(s) 364, 520
NsiI ATGCAT 1 cut(s) 565
PagI TCATGA 1 cut(s) 126
PaqCI CACCTGC 1 cut(s) 541
PctI GAATGC 1 cut(s) 315
PdmI GAANNNNTTC 1 cut(s) 472
PkrI GCNGC 1 cut(s) 213
PshAI GACNNNNGTC 1 cut(s) 362
Psp124BI GAGCTC 1 cut(s) 12
PspFI CCCAGC 1 cut(s) 214
PspPI GGNCC 1 cut(s) 324
PstI CTGCAG 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 61
PvuII CAGCTG 1 cut(s) 214
RsaI GTAC 4 cut(s) 60, 170, 230, 416
RsaNI GTAC 4 cut(s) 59, 169, 229, 415
SacI GAGCTC 1 cut(s) 12
SaqAI TTAA 1 cut(s) 35
SatI GCNGC 1 cut(s) 212
Sau96I GGNCC 1 cut(s) 324
SduI GDGCHC 1 cut(s) 12
SfaNI GCATC 1 cut(s) 550
SfcI CTRYAG 1 cut(s) 53
SmlI CTYRAG 1 cut(s) 525
SmoI CTYRAG 1 cut(s) 525
Sse9I AATT 1 cut(s) 107
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 1 cut(s) 12
StyI CCWWGG 1 cut(s) 95
TaaI ACNGT 2 cut(s) 90, 520
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 77
TasI AATT 1 cut(s) 107
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 3 cut(s) 27, 108, 510
TseFI GTSAC 2 cut(s) 364, 520
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 2 cut(s) 364, 520
TspDTI ATGAA 2 cut(s) 115, 143
TspGWI ACGGA 3 cut(s) 259, 297, 374
TspRI CASTG 3 cut(s) 27, 108, 510
XmnI GAANNNNTTC 1 cut(s) 472
XspI CTAG 1 cut(s) 353
Zsp2I ATGCAT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.