pycom14g00170

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006099
Physical Location & Seq
Forward (+)
97456 .. 103435
5980 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
ATGGAAGTGTTCAGGACGGCCATATTGCAGCCCGGTCCACCTGAAAGGTTTGCCCTTCAGACAGTTCAACAAGTTATACAACCTCAGAAACAAACAAAGTTAGTCCAAGATGAGAATCAATTGCTAGAAAATATCCTTCGGACATTGCTTCAGGAACTGGTGTCTTCTGCAGTACAGTCTGGTGAGCAGATAATGCAGTATGGTCAATCAATTGATGCTGGAGAAACTGCCCAAGGACATATTCCACGTCTTCTTGACGTTGTGTTGTATCTTTGTGAGAAAGAGCACGTCGAAGGCGGCATGATCTTTCAGCTGTTGGAAGACTTAACAGAAATGTCAACTATGAGAAATTGCAAAGACGTTTTCGGTTATATTGAGAGTAAACAAGATATATTAGGAAAGCCTGAGCTTTTTGCTCGAGGAAAACTTGTGATGTTGAGAACATGCAATCAACTTCTTCGTCGTCTGTCAAAGGCAAATGACGTGGTATTTTGTGGACGAATTCTAATGTTTTTGGCACACTTTTTCCCATTATCTGAGCGTTCAGCTGTTAATATAAAAGGAGTTTTTAACACATCAAACGAGACAAAATATGAGAAAGACCCCCCTGATGGAATTTCCATCGATTTCAACTTTTACAAAACCTTTTGGAGTTTACAGGAAAGCTTCTGTAACCCTCCTTCCTTAACTCTTGGTCCTACCAAGTGGAAGAAATTTACGTCTGGTTTAATGGTTGTTTTGAATACCTTTGAAGCTCAACCATTAAGTGATGAAGAGGGAGATGCCAATAACTTGGAGGAGGAAGCAACAAATTTTAGCATAAAATATCTTACAAGCAGTAAACTGATGGGTTTGGAGTTGAAGGATCCCAGTTTCCGACGTCACATTCTTGTGCAGTGCCTCATATTGTTCGATTATCTGAAGGCCCCGGGAAAAAGTGAAAAAGATTTACCTTCTGAAAGCATGAAAGAGGAAATAAAATCTTGTGAGGAGCGTGTCAAAAAGCTGCTTGAGATGACTCCGCCCAAAGGGAAGGAATTTCTTAATAAAATTGAGCATATATTGGAACGTGAAAGGAATTGGGTATGGTGGAAATGTGACGGTTGTCCCCCATTTGAGAAGCAGCCAATGGAGAAAAAGGTGATCCAAGAAGGAGCCAAAAAGCGAACCCTGCTACCCAAATGCTGCCCTGATACCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0023052 GO:0030422 GO:0031047 GO:0031050 GO:0031503 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035556 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071369 GO:0071407 GO:0071426 GO:0071427 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098542 GO:1901360 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
ko03013 ko03040 map03013 map03040
Pfam Domains
Protein Families
efThoc1

Protein Analysis

401

Amino Acids

45.88

Weight (kDa)

5.93

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
efThoc1 PF11957 83 - 387 1.1e-80 THO complex subunit 1 transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 883
AciI CCGC 2 cut(s) 297, 1022
AclWI GGATC 3 cut(s) 860, 873, 1138
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 5 cut(s) 501, 615, 713, 811, 1037
AcuI CTGAAG 3 cut(s) 41, 134, 941
AcyI GRCGYC 1 cut(s) 880
AfaI GTAC 1 cut(s) 174
AfiI CCNNNNNNNGG 2 cut(s) 611, 1028
AgsI TTSAA 5 cut(s) 68, 631, 742, 752, 862
AjiI CACGTC 3 cut(s) 248, 289, 484
AjuI GAANNNNNNNTTGG 2 cut(s) 225, 257
AluBI AGCT 7 cut(s) 313, 409, 548, 666, 755, 1006, 1200
AluI AGCT 7 cut(s) 313, 409, 548, 666, 755, 1006, 1200
Alw21I GWGCWC 1 cut(s) 288
Alw26I GTCTC 1 cut(s) 578
AlwI GGATC 3 cut(s) 860, 873, 1138
AlwNI CAGNNNCTG 1 cut(s) 157
Ama87I CYCGRG 2 cut(s) 417, 928
AoxI GGCC 2 cut(s) 18, 924
ApeKI GCWGC 4 cut(s) 28, 1006, 1123, 1185
ApoI RAATTY 5 cut(s) 501, 615, 713, 811, 1037
AspS9I GGNCC 3 cut(s) 35, 695, 925
AsuC2I CCSGG 3 cut(s) 33, 929, 930
AsuHPI GGTGA 2 cut(s) 194, 1153
AvaI CYCGRG 2 cut(s) 417, 928
AvaII GGWCC 2 cut(s) 35, 695
BamHI GGATCC 1 cut(s) 865
BarI GAAGNNNNNNTAC 2 cut(s) 664, 696
BbsI GAAGAC 3 cut(s) 156, 242, 327
Bbv12I GWGCWC 1 cut(s) 288
BbvI GCAGC 4 cut(s) 40, 993, 1135, 1172
BccI CCATC 3 cut(s) 605, 629, 841
BceAI ACGGC 1 cut(s) 33
BcnI CCSGG 3 cut(s) 33, 929, 930
BcoDI GTCTC 1 cut(s) 578
BfaI CTAG 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 168
BisI GCNGC 5 cut(s) 29, 298, 1007, 1124, 1186
BlsI GCNGC 5 cut(s) 30, 299, 1008, 1125, 1187
Bme1390I CCNGG 3 cut(s) 33, 929, 930
Bme18I GGWCC 2 cut(s) 35, 695
BmeT110I CYCGRG 2 cut(s) 417, 928
BmgBI CACGTC 3 cut(s) 248, 289, 484
BmgT120I GGNCC 3 cut(s) 35, 695, 925
BmiI GGNNCC 3 cut(s) 867, 927, 1156
BmrFI CCNGG 3 cut(s) 33, 929, 930
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 2 cut(s) 205, 772
BmuI ACTGGG 1 cut(s) 864
BoxI GACNNNNGTC 1 cut(s) 1104
BpiI GAAGAC 3 cut(s) 156, 242, 327
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 1031
BpuMI CCSGG 3 cut(s) 33, 929, 930
Bsa29I ATCGAT 1 cut(s) 624
BsaBI GATNNNNATC 1 cut(s) 114
BsaHI GRCGYC 1 cut(s) 880
BsaJI CCNNGG 3 cut(s) 232, 927, 928
Bsc4I CCNNNNNNNGG 2 cut(s) 611, 1028
Bse1I ACTGG 2 cut(s) 162, 870
Bse3DI GCAATG 1 cut(s) 143
Bse8I GATNNNNATC 1 cut(s) 114
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 3 cut(s) 232, 927, 928
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 2 cut(s) 611, 1028
BseMI GCAATG 1 cut(s) 143
BseMII CTCAG 3 cut(s) 98, 396, 528
BseNI ACTGG 2 cut(s) 162, 870
BseRI GAGGAG 2 cut(s) 812, 1004
BseXI GCAGC 4 cut(s) 40, 993, 1135, 1172
BsgI GTGCAG 1 cut(s) 914
BshFI GGCC 2 cut(s) 20, 926
BshVI ATCGAT 1 cut(s) 624
BsiHKAI GWGCWC 1 cut(s) 288
BsiHKCI CYCGRG 2 cut(s) 417, 928
BsiSI CCGG 2 cut(s) 33, 929
BslFI GGGAC 1 cut(s) 1092
BslI CCNNNNNNNGG 2 cut(s) 611, 1028
BsmAI GTCTC 1 cut(s) 578
BsmFI GGGAC 1 cut(s) 1092
BsnI GGCC 2 cut(s) 20, 926
BsoBI CYCGRG 2 cut(s) 417, 928
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 3 cut(s) 303, 865, 1143
BspACI CCGC 2 cut(s) 297, 1022
BspANI GGCC 2 cut(s) 20, 926
BspCNI CTCAG 3 cut(s) 97, 397, 529
BspDI ATCGAT 1 cut(s) 624
BspLI GGNNCC 3 cut(s) 867, 927, 1156
BspMAI CTGCAG 1 cut(s) 172
BspPI GGATC 3 cut(s) 860, 873, 1138
BsrDI GCAATG 1 cut(s) 143
BsrI ACTGG 2 cut(s) 162, 870
BssECI CCNNGG 3 cut(s) 232, 927, 928
BssMI GATC 3 cut(s) 303, 865, 1143
BssNI GRCGYC 1 cut(s) 880
BssT1I CCWWGG 1 cut(s) 232
Bst4CI ACNGT 3 cut(s) 64, 177, 1103
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 1 cut(s) 880
BstAPI GCANNNNNTGC 1 cut(s) 193
BstDEI CTNAG 3 cut(s) 84, 405, 537
BstKTI GATC 3 cut(s) 306, 868, 1146
BstMAI GTCTC 1 cut(s) 578
BstMBI GATC 3 cut(s) 303, 865, 1143
BstMWI GCNNNNNNNGC 2 cut(s) 193, 1171
BstNSI RCATGY 1 cut(s) 447
BstPAI GACNNNNGTC 1 cut(s) 1104
BstSCI CCNGG 3 cut(s) 31, 927, 928
BstSFI CTRYAG 1 cut(s) 168
BstV1I GCAGC 4 cut(s) 40, 993, 1135, 1172
BstV2I GAAGAC 3 cut(s) 156, 242, 327
BstX2I RGATCY 1 cut(s) 865
BstXI CCANNNNNNTGG 1 cut(s) 793
BstYI RGATCY 1 cut(s) 865
Bsu15I ATCGAT 1 cut(s) 624
BsuRI GGCC 2 cut(s) 20, 926
BsuTUI ATCGAT 1 cut(s) 624
BtrI CACGTC 3 cut(s) 248, 289, 484
BtsI GCAGTG 1 cut(s) 902
BtsIMutI CAGTG 1 cut(s) 902
CaiI CAGNNNCTG 1 cut(s) 157
Cfr13I GGNCC 3 cut(s) 35, 695, 925
Cfr9I CCCGGG 1 cut(s) 928
ClaI ATCGAT 1 cut(s) 624
Csp6I GTAC 1 cut(s) 173
CspCI CAANNNNNGTGG 2 cut(s) 465, 500
CviAII CATG 3 cut(s) 301, 444, 964
CviQI GTAC 1 cut(s) 173
DdeI CTNAG 3 cut(s) 84, 405, 537
DpnI GATC 3 cut(s) 305, 867, 1145
DpnII GATC 3 cut(s) 303, 865, 1143
EaeI YGGCCR 1 cut(s) 18
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
EciI GGCGGA 1 cut(s) 1011
Eco130I CCWWGG 1 cut(s) 232
Eco47I GGWCC 2 cut(s) 35, 695
Eco57I CTGAAG 3 cut(s) 41, 134, 941
Eco88I CYCGRG 2 cut(s) 417, 928
EcoO109I RGGNCCY 1 cut(s) 925
EcoRI GAATTC 1 cut(s) 501
EcoT14I CCWWGG 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 232
FaeI CATG 3 cut(s) 304, 447, 967
FaqI GGGAC 1 cut(s) 1092
FatI CATG 3 cut(s) 300, 443, 963
Fnu4HI GCNGC 5 cut(s) 29, 298, 1007, 1124, 1186
Fsp4HI GCNGC 5 cut(s) 29, 298, 1007, 1124, 1186
FspBI CTAG 1 cut(s) 125
GluI GCNGC 5 cut(s) 29, 298, 1007, 1124, 1186
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 2 cut(s) 20, 926
HapII CCGG 2 cut(s) 33, 929
Hin1I GRCGYC 1 cut(s) 880
Hin1II CATG 3 cut(s) 304, 447, 967
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 1 cut(s) 664
HinfI GANTC 2 cut(s) 115, 1018
HpaII CCGG 2 cut(s) 33, 929
HphI GGTGA 2 cut(s) 194, 1153
Hpy166II GTNNAC 6 cut(s) 38, 339, 383, 497, 656, 842
Hpy188I TCNGA 7 cut(s) 60, 87, 141, 538, 878, 921, 958
Hpy188III TCNNGA 3 cut(s) 13, 152, 254
Hpy8I GTNNAC 6 cut(s) 38, 339, 383, 497, 656, 842
Hpy99I CGWCG 3 cut(s) 293, 465, 882
HpyAV CCTTC 9 cut(s) 65, 146, 287, 690, 856, 916, 963, 1027, 1145
HpyCH4III ACNGT 3 cut(s) 64, 177, 1103
HpyCH4IV ACGT 8 cut(s) 247, 258, 288, 360, 483, 719, 880, 1069
HpyCH4V TGCA 6 cut(s) 28, 170, 196, 354, 447, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 193, 1171
HpyF3I CTNAG 3 cut(s) 84, 405, 537
HpySE526I ACGT 8 cut(s) 247, 258, 288, 360, 483, 719, 880, 1069
Hsp92I GRCGYC 1 cut(s) 880
Hsp92II CATG 3 cut(s) 304, 447, 967
Kzo9I GATC 3 cut(s) 303, 865, 1143
LmnI GCTCC 2 cut(s) 991, 1154
Lsp1109I GCAGC 4 cut(s) 40, 993, 1135, 1172
LweI GCATC 2 cut(s) 205, 772
MaeI CTAG 1 cut(s) 125
MaeII ACGT 8 cut(s) 247, 258, 288, 360, 483, 719, 880, 1069
MaeIII GTNAC 3 cut(s) 671, 881, 1097
MalI GATC 3 cut(s) 305, 867, 1145
MboI GATC 3 cut(s) 303, 865, 1143
MboII GAAGA 6 cut(s) 156, 242, 332, 449, 721, 785
MfeI CAATTG 2 cut(s) 119, 210
MflI RGATCY 1 cut(s) 865
MhlI GDGCHC 1 cut(s) 288
MlyI GAGTC 1 cut(s) 1012
MmeI TCCRAC 2 cut(s) 297, 901
MnlI CCTC 9 cut(s) 93, 413, 687, 769, 790, 793, 911, 964, 982
MseI TTAA 7 cut(s) 326, 552, 570, 686, 728, 764, 1044
MslI CAYNNNNRTG 1 cut(s) 890
MspA1I CMGCKG 3 cut(s) 313, 548, 1200
MspI CCGG 2 cut(s) 33, 929
MspR9I CCNGG 3 cut(s) 33, 929, 930
MunI CAATTG 2 cut(s) 119, 210
MwoI GCNNNNNNNGC 2 cut(s) 193, 1171
NciI CCSGG 3 cut(s) 33, 929, 930
NdeII GATC 3 cut(s) 303, 865, 1143
NlaIII CATG 3 cut(s) 304, 447, 967
NlaIV GGNNCC 3 cut(s) 867, 927, 1156
NmuCI GTSAC 2 cut(s) 881, 1097
NspI RCATGY 1 cut(s) 447
PaeR7I CTCGAG 1 cut(s) 417
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 5 cut(s) 30, 299, 1008, 1125, 1187
PleI GAGTC 1 cut(s) 1012
PpsI GAGTC 1 cut(s) 1012
PshAI GACNNNNGTC 1 cut(s) 1104
PspN4I GGNNCC 3 cut(s) 867, 927, 1156
PspPI GGNCC 3 cut(s) 35, 695, 925
PspXI VCTCGAGB 1 cut(s) 417
PstI CTGCAG 1 cut(s) 172
PstNI CAGNNNCTG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 865
PvuII CAGCTG 3 cut(s) 313, 548, 1200
RsaI GTAC 1 cut(s) 174
RsaNI GTAC 1 cut(s) 173
RseI CAYNNNNRTG 1 cut(s) 890
SaqAI TTAA 7 cut(s) 326, 552, 570, 686, 728, 764, 1044
SatI GCNGC 5 cut(s) 29, 298, 1007, 1124, 1186
Sau3AI GATC 3 cut(s) 303, 865, 1143
Sau96I GGNCC 3 cut(s) 35, 695, 925
SchI GAGTC 1 cut(s) 1012
ScrFI CCNGG 3 cut(s) 33, 929, 930
SduI GDGCHC 1 cut(s) 288
SfaNI GCATC 2 cut(s) 205, 772
SfcI CTRYAG 1 cut(s) 168
Sfr274I CTCGAG 1 cut(s) 417
SinI GGWCC 2 cut(s) 35, 695
SlaI CTCGAG 1 cut(s) 417
SmaI CCCGGG 1 cut(s) 930
SmiMI CAYNNNNRTG 1 cut(s) 890
SmlI CTYRAG 2 cut(s) 417, 1010
SmoI CTYRAG 2 cut(s) 417, 1010
SsiI CCGC 2 cut(s) 297, 1022
SspMI CTAG 1 cut(s) 125
StyD4I CCNGG 3 cut(s) 31, 927, 928
StyI CCWWGG 1 cut(s) 232
TaaI ACNGT 3 cut(s) 64, 177, 1103
TaiI ACGT 8 cut(s) 250, 261, 291, 363, 486, 722, 883, 1072
TaqI TCGA 4 cut(s) 291, 418, 624, 912
TatI WGTACW 1 cut(s) 172
TauI GCSGC 1 cut(s) 300
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 7 cut(s) 326, 552, 570, 686, 728, 764, 1044
Tru9I TTAA 7 cut(s) 326, 552, 570, 686, 728, 764, 1044
TscAI CASTG 1 cut(s) 902
TseFI GTSAC 2 cut(s) 881, 1097
TseI GCWGC 4 cut(s) 28, 1006, 1123, 1185
Tsp45I GTSAC 2 cut(s) 881, 1097
TspDTI ATGAA 2 cut(s) 786, 980
TspMI CCCGGG 1 cut(s) 928
TspRI CASTG 1 cut(s) 902
VpaK11BI GGWCC 2 cut(s) 35, 695
XapI RAATTY 5 cut(s) 501, 615, 713, 811, 1037
XceI RCATGY 1 cut(s) 447
XhoI CTCGAG 1 cut(s) 417
XmaI CCCGGG 1 cut(s) 928
XspI CTAG 1 cut(s) 125
ZraI GACGTC 1 cut(s) 881
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.