pycom14g00340

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015701
Physical Location & Seq
Forward (+)
212926 .. 214037
1112 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1029 bp
ATGGATTCTAATGGTGAATATGAGACAGAAGCAGAGACCATGGCTCTTTGTCGGGCAAGATCTTCTCTTCGTCGGGCAAGATCTTCTGTTGTAGAATCTTCTGATTCTGAGCCCGAGTGGAGACCCCAAGCTAGACCAGCTAAAGCAACAAATCCTCCTCCCAAAGCACAAGGCCCTTCCCAGGCCACCAGTGAGGGCTTGGGCAGGTCTCCTCAACTTCCTCAAGCTTCTGGAATTTTAGGGATTCCAATCCCTCGGCCTCCAAAGGTTGTAGCTGCAGAATCCTTTCCTGCTATTCTTGCTGATGTTACCCATGCTGCCCCTGCTGCTCTTGCTTTTGATGGTCCTAGCGTGATGCGTCCTCCTTTGGCTTCTCTTTCAGCCACGACCTCTTTGTCTGAACTAGTCAGAAAGTTCAGGCAAATTGTAACGAAGATAAGATCCACAAGGCGTCCTTCTGAGCCTCAACATCTTCAAGATCAGCGTAGGATCTTCAGAGAATGGATGCAAAGGGACTTTTCTGCCTTCTTTAGCCTTAAGGCTCTTCAAGATGCTGAGATAGCTCTCACTAAATTATACCAAGCCCACCAGATGACTAAGGTGCAATATGAGTCCTTCCTTTCCTTCTTTGAAAATCTAAGGGCCTTAAGGGATCAACATCTTAAGGCCGAGAGGCAAGCCAACAGGGTGAGGTGCTACAAGGAGAAGCACACTCAGACCTCCACTACTCTGCAACAACTGGTGGAGGAGGGCTCGTCCATGGAGGATCGGATAATAGTGGTAGTTGCTGAGATCCAAGAACTGGAGGAACAACTTTCTGCTCTAAATGCTGAGCAGATGACTCTCTCGAGCAAGCTATACAAGAAGATGGAGGAAGTCAAGAAAGTAAACCACGAAGTGGAGGAGTTTGAAGCCCAACTAGCCAACAACAATATAGCTTTAGAAGAGCCGGGTTGTATTTTTACCATCATGCAGACTTATCATTCTAGGATAGCTGCCTTGGCTAAAGATGTAAACTTGTTAATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
HSP20

Protein Analysis

343

Amino Acids

38.44

Weight (kDa)

7.08

Isoelectric Point (pI)

57.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015701)

Species Orthologous Gene IDs
malus_domestica MD14G1005100.v1.1 MD14G1005200.v1.1
prunus_persica Prupe.7G008400_v2.0.a1
pyrus_communis pycom14g00340 pycom14g00350 pycom14g00360 pycom14g00370
rosa_chinensis RchiOBHm_Chr3g0496541
rosa_laevigata RLG00000022520
rosa_multiflora Rmu_sc0000741.1_g000008
rosa_roxburghii Rroxscaffold_6G00388950
rosa_rugosa Rorug03G0283900
rosa_samantha Rh3BG365300 Rh3CG362600 Rh3DG365700
rosa_wichuraiana Rw3G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 394
Acc36I ACCTGC 1 cut(s) 195
AccB7I CCANNNNNTGG 2 cut(s) 802, 898
AclWI GGATC 5 cut(s) 435, 497, 660, 774, 787
AcsI RAATTY 1 cut(s) 234
AcuI CTGAAG 1 cut(s) 478
AcyI GRCGYC 1 cut(s) 451
AdeI CACNNNGTG 1 cut(s) 898
AfiI CCNNNNNNNGG 3 cut(s) 181, 802, 898
AflII CTTAAG 3 cut(s) 536, 646, 662
AgsI TTSAA 4 cut(s) 476, 548, 632, 911
AhlI ACTAGT 1 cut(s) 403
AjnI CCWGG 1 cut(s) 180
AluBI AGCT 8 cut(s) 131, 140, 227, 275, 563, 856, 938, 995
AluI AGCT 8 cut(s) 131, 140, 227, 275, 563, 856, 938, 995
Alw26I GTCTC 4 cut(s) 17, 29, 115, 213
AlwI GGATC 5 cut(s) 435, 497, 660, 774, 787
Ama87I CYCGRG 2 cut(s) 113, 847
AoxI GGCC 5 cut(s) 172, 183, 257, 642, 666
ApeKI GCWGC 4 cut(s) 275, 317, 326, 995
ApoI RAATTY 1 cut(s) 234
Asp700I GAANNNNTTC 1 cut(s) 285
AspS9I GGNCC 3 cut(s) 173, 344, 642
AsuC2I CCSGG 1 cut(s) 951
AsuHPI GGTGA 2 cut(s) 26, 700
AvaI CYCGRG 2 cut(s) 113, 847
AvaII GGWCC 1 cut(s) 344
BanII GRGCYC 2 cut(s) 114, 755
BbvI GCAGC 4 cut(s) 262, 304, 313, 982
BccI CCATC 3 cut(s) 335, 862, 974
BciT130I CCWGG 1 cut(s) 182
BcnI CCSGG 1 cut(s) 951
BcoDI GTCTC 4 cut(s) 17, 29, 115, 213
BcuI ACTAGT 1 cut(s) 403
BfaI CTAG 5 cut(s) 132, 348, 404, 920, 987
BfmI CTRYAG 1 cut(s) 276
BfrI CTTAAG 3 cut(s) 536, 646, 662
BfuAI ACCTGC 1 cut(s) 195
BglII AGATCT 2 cut(s) 59, 80
BisI GCNGC 4 cut(s) 276, 318, 327, 996
BlpI GCTNAGC 1 cut(s) 831
BlsI GCNGC 4 cut(s) 277, 319, 328, 997
Bme1390I CCNGG 2 cut(s) 182, 951
Bme18I GGWCC 1 cut(s) 344
BmeT110I CYCGRG 2 cut(s) 113, 847
BmgT120I GGNCC 3 cut(s) 173, 344, 642
BmrFI CCNGG 2 cut(s) 182, 951
BmsI GCATC 3 cut(s) 345, 495, 541
BplI GAGNNNNNCTC 2 cut(s) 737, 769
BpmI CTGGAG 1 cut(s) 824
Bpu1102I GCTNAGC 1 cut(s) 831
BpuEI CTTGAG 1 cut(s) 207
BpuMI CCSGG 1 cut(s) 951
BsaBI GATNNNNATC 2 cut(s) 248, 657
BsaHI GRCGYC 1 cut(s) 451
BsaI GGTCTC 3 cut(s) 29, 115, 213
BsaJI CCNNGG 5 cut(s) 39, 180, 254, 759, 999
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 740, 770
Bsc4I CCNNNNNNNGG 3 cut(s) 181, 802, 898
Bse1I ACTGG 3 cut(s) 189, 744, 807
Bse8I GATNNNNATC 2 cut(s) 248, 657
BseBI CCWGG 1 cut(s) 182
BseDI CCNNGG 5 cut(s) 39, 180, 254, 759, 999
BseGI GGATG 1 cut(s) 510
BseJI GATNNNNATC 2 cut(s) 248, 657
BseLI CCNNNNNNNGG 3 cut(s) 181, 802, 898
BseMII CTCAG 6 cut(s) 99, 450, 546, 728, 780, 822
BseNI ACTGG 3 cut(s) 189, 744, 807
BseRI GAGGAG 4 cut(s) 147, 201, 761, 917
BseXI GCAGC 4 cut(s) 262, 304, 313, 982
BshFI GGCC 5 cut(s) 174, 185, 259, 644, 668
BsiHKCI CYCGRG 2 cut(s) 113, 847
BsiSI CCGG 1 cut(s) 950
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 3 cut(s) 181, 802, 898
BsmAI GTCTC 4 cut(s) 17, 29, 115, 213
BsmFI GGGAC 1 cut(s) 527
BsnI GGCC 5 cut(s) 174, 185, 259, 644, 668
Bso31I GGTCTC 3 cut(s) 29, 115, 213
BsoBI CYCGRG 2 cut(s) 113, 847
Bsp1286I GDGCHC 2 cut(s) 114, 755
Bsp143I GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 2 cut(s) 39, 759
BspANI GGCC 5 cut(s) 174, 185, 259, 644, 668
BspCNI CTCAG 6 cut(s) 100, 451, 547, 727, 781, 823
BspMAI CTGCAG 1 cut(s) 280
BspMI ACCTGC 1 cut(s) 195
BspPI GGATC 5 cut(s) 435, 497, 660, 774, 787
BspQI GCTCTTC 2 cut(s) 549, 939
BspTI CTTAAG 3 cut(s) 536, 646, 662
BspTNI GGTCTC 3 cut(s) 29, 115, 213
BsrI ACTGG 3 cut(s) 189, 744, 807
BssECI CCNNGG 5 cut(s) 39, 180, 254, 759, 999
BssMI GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
BssNI GRCGYC 1 cut(s) 451
BssT1I CCWWGG 3 cut(s) 39, 759, 999
Bst2UI CCWGG 1 cut(s) 182
Bst6I CTCTTC 3 cut(s) 72, 549, 939
BstACI GRCGYC 1 cut(s) 451
BstAFI CTTAAG 3 cut(s) 536, 646, 662
BstC8I GCNNGC 2 cut(s) 678, 854
BstDEI CTNAG 8 cut(s) 108, 459, 555, 597, 638, 714, 789, 831
BstDSI CCRYGG 2 cut(s) 39, 759
BstF5I GGATG 1 cut(s) 510
BstKTI GATC 8 cut(s) 62, 83, 443, 481, 492, 655, 769, 795
BstMAI GTCTC 4 cut(s) 17, 29, 115, 213
BstMBI GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
BstMWI GCNNNNNNNGC 9 cut(s) 137, 299, 323, 326, 332, 560, 827, 920, 1001
BstNI CCWGG 1 cut(s) 182
BstSCI CCNGG 2 cut(s) 180, 949
BstSFI CTRYAG 1 cut(s) 276
BstV1I GCAGC 4 cut(s) 262, 304, 313, 982
BstX2I RGATCY 5 cut(s) 59, 80, 440, 489, 792
BstYI RGATCY 5 cut(s) 59, 80, 440, 489, 792
BsuRI GGCC 5 cut(s) 174, 185, 259, 644, 668
BtgI CCRYGG 2 cut(s) 39, 759
BtsCI GGATG 1 cut(s) 510
BtsIMutI CAGTG 1 cut(s) 196
BveI ACCTGC 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 678, 854
Cfr13I GGNCC 3 cut(s) 173, 344, 642
CseI GACGC 2 cut(s) 347, 440
CviAII CATG 4 cut(s) 40, 314, 760, 970
DdeI CTNAG 8 cut(s) 108, 459, 555, 597, 638, 714, 789, 831
DpnI GATC 8 cut(s) 61, 82, 442, 480, 491, 654, 768, 794
DpnII GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
DraIII CACNNNGTG 1 cut(s) 898
DrdI GACNNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 394
Eam1104I CTCTTC 3 cut(s) 72, 549, 939
EarI CTCTTC 3 cut(s) 72, 549, 939
Eco130I CCWWGG 3 cut(s) 39, 759, 999
Eco24I GRGCYC 2 cut(s) 114, 755
Eco31I GGTCTC 3 cut(s) 29, 115, 213
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 478
Eco88I CYCGRG 2 cut(s) 113, 847
EcoO109I RGGNCCY 2 cut(s) 173, 642
EcoRII CCWGG 1 cut(s) 180
EcoT14I CCWWGG 3 cut(s) 39, 759, 999
EcoT38I GRGCYC 2 cut(s) 114, 755
ErhI CCWWGG 3 cut(s) 39, 759, 999
FaeI CATG 4 cut(s) 43, 317, 763, 973
FaiI YATR 9 cut(s) 21, 41, 315, 577, 609, 761, 859, 935, 971
FalI AAGNNNNNCTT 2 cut(s) 439, 471
FaqI GGGAC 1 cut(s) 527
FatI CATG 4 cut(s) 39, 313, 759, 969
Fnu4HI GCNGC 4 cut(s) 276, 318, 327, 996
FokI GGATG 1 cut(s) 517
FriOI GRGCYC 2 cut(s) 114, 755
Fsp4HI GCNGC 4 cut(s) 276, 318, 327, 996
FspBI CTAG 5 cut(s) 132, 348, 404, 920, 987
GluI GCNGC 4 cut(s) 276, 318, 327, 996
GsuI CTGGAG 1 cut(s) 824
HaeIII GGCC 5 cut(s) 174, 185, 259, 644, 668
HapII CCGG 1 cut(s) 950
HgaI GACGC 2 cut(s) 347, 440
Hin1I GRCGYC 1 cut(s) 451
Hin1II CATG 4 cut(s) 43, 317, 763, 973
HindIII AAGCTT 1 cut(s) 225
HinfI GANTC 7 cut(s) 5, 95, 104, 244, 281, 611, 841
HpaII CCGG 1 cut(s) 950
HphI GGTGA 2 cut(s) 26, 700
Hpy166II GTNNAC 2 cut(s) 889, 1015
Hpy188I TCNGA 8 cut(s) 103, 109, 400, 410, 460, 497, 717, 771
Hpy188III TCNNGA 5 cut(s) 231, 476, 548, 847, 880
Hpy8I GTNNAC 2 cut(s) 889, 1015
Hpy99I CGWCG 1 cut(s) 75
HpyAV CCTTC 5 cut(s) 186, 465, 535, 625, 634
HpyCH4V TGCA 5 cut(s) 278, 508, 604, 733, 973
HpyF10VI GCNNNNNNNGC 9 cut(s) 137, 299, 323, 326, 332, 560, 827, 920, 1001
HpyF3I CTNAG 8 cut(s) 108, 459, 555, 597, 638, 714, 789, 831
Hsp92I GRCGYC 1 cut(s) 451
Hsp92II CATG 4 cut(s) 43, 317, 763, 973
Kzo9I GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
LguI GCTCTTC 2 cut(s) 549, 939
Lsp1109I GCAGC 4 cut(s) 262, 304, 313, 982
LweI GCATC 3 cut(s) 345, 495, 541
MaeI CTAG 5 cut(s) 132, 348, 404, 920, 987
MaeIII GTNAC 2 cut(s) 307, 427
MalI GATC 8 cut(s) 61, 82, 442, 480, 491, 654, 768, 794
MboI GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
MflI RGATCY 5 cut(s) 59, 80, 440, 489, 792
MhlI GDGCHC 2 cut(s) 114, 755
MluCI AATT 4 cut(s) 234, 423, 572, 1023
MlyI GAGTC 2 cut(s) 620, 835
MroXI GAANNNNTTC 1 cut(s) 285
MseI TTAA 4 cut(s) 537, 647, 663, 1022
MspCI CTTAAG 3 cut(s) 536, 646, 662
MspI CCGG 1 cut(s) 950
MspR9I CCNGG 2 cut(s) 182, 951
MvaI CCWGG 1 cut(s) 182
MwoI GCNNNNNNNGC 9 cut(s) 137, 299, 323, 326, 332, 560, 827, 920, 1001
NciI CCSGG 1 cut(s) 951
NcoI CCATGG 2 cut(s) 39, 759
NdeII GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
NlaIII CATG 4 cut(s) 43, 317, 763, 973
NmeAIII GCCGAG 2 cut(s) 235, 694
PaeR7I CTCGAG 1 cut(s) 847
PciSI GCTCTTC 2 cut(s) 549, 939
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 5 cut(s) 5, 95, 104, 244, 281
PflMI CCANNNNNTGG 2 cut(s) 802, 898
PkrI GCNGC 4 cut(s) 277, 319, 328, 997
PleI GAGTC 2 cut(s) 619, 835
PpsI GAGTC 2 cut(s) 619, 835
Psp6I CCWGG 1 cut(s) 180
PspGI CCWGG 1 cut(s) 180
PspPI GGNCC 3 cut(s) 173, 344, 642
PstI CTGCAG 1 cut(s) 280
PsuI RGATCY 5 cut(s) 59, 80, 440, 489, 792
SapI GCTCTTC 2 cut(s) 549, 939
SaqAI TTAA 4 cut(s) 537, 647, 663, 1022
SatI GCNGC 4 cut(s) 276, 318, 327, 996
Sau3AI GATC 8 cut(s) 59, 80, 440, 478, 489, 652, 766, 792
Sau96I GGNCC 3 cut(s) 173, 344, 642
SchI GAGTC 2 cut(s) 620, 835
ScrFI CCNGG 2 cut(s) 182, 951
SduI GDGCHC 2 cut(s) 114, 755
SfaNI GCATC 3 cut(s) 345, 495, 541
SfcI CTRYAG 1 cut(s) 276
Sfr274I CTCGAG 1 cut(s) 847
SinI GGWCC 1 cut(s) 344
SlaI CTCGAG 1 cut(s) 847
SmlI CTYRAG 5 cut(s) 222, 536, 646, 662, 847
SmoI CTYRAG 5 cut(s) 222, 536, 646, 662, 847
SpeI ACTAGT 1 cut(s) 403
Sse9I AATT 4 cut(s) 234, 423, 572, 1023
SspMI CTAG 5 cut(s) 132, 348, 404, 920, 987
StyD4I CCNGG 2 cut(s) 180, 949
StyI CCWWGG 3 cut(s) 39, 759, 999
TaqI TCGA 1 cut(s) 848
TasI AATT 4 cut(s) 234, 423, 572, 1023
TfiI GAWTC 5 cut(s) 5, 95, 104, 244, 281
Tru1I TTAA 4 cut(s) 537, 647, 663, 1022
Tru9I TTAA 4 cut(s) 537, 647, 663, 1022
TscAI CASTG 1 cut(s) 196
TseI GCWGC 4 cut(s) 275, 317, 326, 995
TspRI CASTG 1 cut(s) 196
Van91I CCANNNNNTGG 2 cut(s) 802, 898
Vha464I CTTAAG 3 cut(s) 536, 646, 662
VpaK11BI GGWCC 1 cut(s) 344
XapI RAATTY 1 cut(s) 234
XcmI CCANNNNNNNNNTGG 1 cut(s) 196
XhoI CTCGAG 1 cut(s) 847
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 5 cut(s) 132, 348, 404, 920, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.