pycom14g00370

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015701
Physical Location & Seq
Forward (+)
235811 .. 237199
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1209 bp
ATGAGTCAGAAGCAAAGACCATGTCGGGCAAGATCTTCTCTTCGTCGGCCAAGATCTTTTGTTGTAGAATCTTTTGATTCTGAGCCCGAGGGGAGGCCCCAAGCTACACCAGCTACATCAACACATCCTTCTCCCAAAGCCATTGGTGAGGGCTCGGGCAGGTTTTCTCAGCCTGTTGCTGAATCCTCTCATTCTGTTCCTACTGAGGTTGCCACTGCTGCTGCTGCTTTTGGTGGCACTAGCGTGATGCCTTCTCTTATGGCTTCTCTTCCAGCTACGACCTCTTTGCCTGAACTAGTTAGAGAGTTCGGGCAAATTAGGATGAAGTTACGATCCCCAAGGCGTCCTTCTGAGACTCAACATCTTCAAGATCAGCGCAGGATCTTCAGAGAATTTATGCATAAGGACTTTTCTGCCTCCTTTAGCCTTAAGGCTCTTCAAGTTGCTGAGAGAGCTCTCACTGAATTATACCAAACCCAGCAGATGACTAAGGTGCAATATGAGTCCTTCATTTCCTTCTTTGAAAATCTAAGGGCCTTGAGGGATCAACATCTTAAAGCCGAAAGGCAAGCCAACAGGGTGAGATGCTACAAGGAGAAGCACACTCAGACCTCCACTAATCTGCAGCAGCTGGTGGAGGAGGGCTTGGCCATGAAGGATTGGATAATAATGGTAGCTGCCGAGATCCAGAAACTGGAGGAACAACTTTATGATCTGAAGGCTGAGCAGATTACTCTCTCAAGCAAGCTATACAAGAAGATCGAGAAAGTGAACATTATACGTTCTTACGAGGATTTTATGCCTTATTGCAAGTTCCTAAAAGAGGAACGATTCGACATTTACCAGATTCCTCTCCATGGTTTCAAGATGGAACAATTCAAGCTATTCTTGAACGACAACAAGGGAACAATAAAGATTGAAGGCCAACGTCCTAAGGGTGGAAGTAGATGGAGCCGCTTCCGCCAAAAATTCAAACTTCCTTGCAATGTTTATAACACTAAGGATATTCAAGCAAGTTTTGGCAATGGTGTCCTTACCATAAGACTGCCAAAAAGGGAACCATTGATGATTTCGAATTCCGACGACAAGTCTGAGCTATTTGTATACAACGGTAAGCTTTTCGGGCTGAAAATCAGCAAAAGAGGAATCCTCGGATCAATTGCACTTGTTGCTGTGGGCGTGGCTGTTGGAGGTTATGTAATCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko01110 ko02026 map00500 map01100 map01110 map02026
Pfam Domains
Protein Families
HSP20

Protein Analysis

403

Amino Acids

45.86

Weight (kDa)

9.76

Isoelectric Point (pI)

55.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 282 - 355 2.5e-07 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015701)

Species Orthologous Gene IDs
malus_domestica MD14G1005100.v1.1 MD14G1005200.v1.1
prunus_persica Prupe.7G008400_v2.0.a1
pyrus_communis pycom14g00340 pycom14g00350 pycom14g00360 pycom14g00370
rosa_chinensis RchiOBHm_Chr3g0496541
rosa_laevigata RLG00000022520
rosa_multiflora Rmu_sc0000741.1_g000008
rosa_roxburghii Rroxscaffold_6G00388950
rosa_rugosa Rorug03G0283900
rosa_samantha Rh3BG365300 Rh3CG362600 Rh3DG365700
rosa_wichuraiana Rw3G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 993
Acc36I ACCTGC 1 cut(s) 150
AccB7I CCANNNNNTGG 1 cut(s) 694
AccI GTMKAC 1 cut(s) 1104
AciI CCGC 2 cut(s) 955, 961
AclWI GGATC 5 cut(s) 327, 389, 552, 679, 1162
AcoI YGGCCR 2 cut(s) 47, 648
AcsI RAATTY 3 cut(s) 392, 968, 1075
AcuI CTGAAG 2 cut(s) 370, 737
AcyI GRCGYC 1 cut(s) 343
AfiI CCNNNNNNNGG 5 cut(s) 93, 694, 823, 857, 938
AflII CTTAAG 1 cut(s) 428
AgsI TTSAA 9 cut(s) 368, 440, 524, 865, 880, 892, 920, 973, 1010
AhdI GACNNNNNGTC 1 cut(s) 1087
AhlI ACTAGT 1 cut(s) 295
AjuI GAANNNNNNNTTGG 2 cut(s) 331, 363
AleI CACNNNNGTG 1 cut(s) 242
Alw21I GWGCWC 1 cut(s) 457
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 5 cut(s) 327, 389, 552, 679, 1162
AlwNI CAGNNNCTG 2 cut(s) 631, 694
Ama87I CYCGRG 2 cut(s) 86, 154
AoxI GGCC 5 cut(s) 47, 95, 534, 648, 922
ApeKI GCWGC 6 cut(s) 218, 221, 224, 625, 628, 677
ApoI RAATTY 3 cut(s) 392, 968, 1075
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 2 cut(s) 96, 534
AsuHPI GGTGA 2 cut(s) 158, 592
AsuII TTCGAA 1 cut(s) 1073
AvaI CYCGRG 2 cut(s) 86, 154
AxyI CCTNAGG 1 cut(s) 933
BalI TGGCCA 1 cut(s) 650
BanII GRGCYC 3 cut(s) 87, 155, 457
Bbv12I GWGCWC 1 cut(s) 457
BbvI GCAGC 6 cut(s) 205, 208, 211, 637, 640, 664
BccI CCATC 2 cut(s) 862, 942
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BcoDI GTCTC 1 cut(s) 347
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 2 cut(s) 240, 296
BfmI CTRYAG 1 cut(s) 623
BfrI CTTAAG 1 cut(s) 428
BfuAI ACCTGC 1 cut(s) 150
BglII AGATCT 2 cut(s) 32, 53
BisI GCNGC 7 cut(s) 219, 222, 225, 626, 629, 678, 955
BlpI GCTNAGC 1 cut(s) 723
BlsI GCNGC 7 cut(s) 220, 223, 226, 627, 630, 679, 956
BmeRI GACNNNNNGTC 1 cut(s) 1087
BmeT110I CYCGRG 2 cut(s) 86, 154
BmgT120I GGNCC 2 cut(s) 96, 534
BmiI GGNNCC 3 cut(s) 98, 953, 1059
BmsI GCATC 2 cut(s) 237, 575
BplI GAGNNNNNCTC 2 cut(s) 1134, 1166
BpmI CTGGAG 1 cut(s) 716
Bpu1102I GCTNAGC 1 cut(s) 723
Bpu14I TTCGAA 1 cut(s) 1073
BpuEI CTTGAG 2 cut(s) 559, 724
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 343
BsaJI CCNNGG 4 cut(s) 87, 338, 856, 1150
BsaXI ACNNNNNCTCC 1 cut(s) 1182
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 694, 823, 857, 938
Bse1I ACTGG 1 cut(s) 699
Bse21I CCTNAGG 1 cut(s) 933
Bse3DI GCAATG 2 cut(s) 991, 1030
Bse8I GATNNNNATC 1 cut(s) 549
BseDI CCNNGG 4 cut(s) 87, 338, 856, 1150
BseGI GGATG 2 cut(s) 124, 327
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 5 cut(s) 93, 694, 823, 857, 938
BseMI GCAATG 2 cut(s) 991, 1030
BseMII CTCAG 8 cut(s) 72, 182, 195, 342, 438, 620, 714, 1083
BseNI ACTGG 1 cut(s) 699
BseRI GAGGAG 1 cut(s) 653
BseXI GCAGC 6 cut(s) 205, 208, 211, 637, 640, 664
BseYI CCCAGC 1 cut(s) 477
BshFI GGCC 5 cut(s) 49, 97, 536, 650, 924
BsiHKAI GWGCWC 1 cut(s) 457
BsiHKCI CYCGRG 2 cut(s) 86, 154
BslI CCNNNNNNNGG 5 cut(s) 93, 694, 823, 857, 938
BsmAI GTCTC 1 cut(s) 347
BsnI GGCC 5 cut(s) 49, 97, 536, 650, 924
BsoBI CYCGRG 2 cut(s) 86, 154
Bsp119I TTCGAA 1 cut(s) 1073
Bsp1286I GDGCHC 3 cut(s) 87, 155, 457
Bsp1720I GCTNAGC 1 cut(s) 723
Bsp19I CCATGG 1 cut(s) 856
BspACI CCGC 2 cut(s) 955, 961
BspANI GGCC 5 cut(s) 49, 97, 536, 650, 924
BspCNI CTCAG 8 cut(s) 73, 181, 196, 343, 439, 619, 715, 1084
BspLI GGNNCC 3 cut(s) 98, 953, 1059
BspMAI CTGCAG 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 150
BspPI GGATC 5 cut(s) 327, 389, 552, 679, 1162
BspQI GCTCTTC 1 cut(s) 441
BspT104I TTCGAA 1 cut(s) 1073
BspTI CTTAAG 1 cut(s) 428
BsrDI GCAATG 2 cut(s) 991, 1030
BsrI ACTGG 1 cut(s) 699
BssECI CCNNGG 4 cut(s) 87, 338, 856, 1150
BssNAI GTATAC 1 cut(s) 1105
BssNI GRCGYC 1 cut(s) 343
BssT1I CCWWGG 2 cut(s) 338, 856
Bst1107I GTATAC 1 cut(s) 1105
Bst4CI ACNGT 1 cut(s) 1112
Bst6I CTCTTC 3 cut(s) 45, 273, 441
BstACI GRCGYC 1 cut(s) 343
BstAFI CTTAAG 1 cut(s) 428
BstAPI GCANNNNNTGC 1 cut(s) 1169
BstBI TTCGAA 1 cut(s) 1073
BstC8I GCNNGC 2 cut(s) 570, 746
BstDSI CCRYGG 1 cut(s) 856
BstENI CCTNNNNNAGG 1 cut(s) 821
BstF5I GGATG 2 cut(s) 124, 327
BstHHI GCGC 1 cut(s) 378
BstMAI GTCTC 1 cut(s) 347
BstMWI GCNNNNNNNGC 7 cut(s) 110, 218, 224, 452, 960, 1123, 1169
BstSFI CTRYAG 1 cut(s) 623
BstV1I GCAGC 6 cut(s) 205, 208, 211, 637, 640, 664
BstX2I RGATCY 4 cut(s) 32, 53, 381, 684
BstYI RGATCY 4 cut(s) 32, 53, 381, 684
BstZ17I GTATAC 1 cut(s) 1105
Bsu36I CCTNAGG 1 cut(s) 933
BsuRI GGCC 5 cut(s) 49, 97, 536, 650, 924
BtgI CCRYGG 1 cut(s) 856
BtsCI GGATG 2 cut(s) 124, 327
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 213, 459
BveI ACCTGC 1 cut(s) 150
Cac8I GCNNGC 2 cut(s) 570, 746
CaiI CAGNNNCTG 2 cut(s) 631, 694
CfoI GCGC 1 cut(s) 378
Cfr13I GGNCC 2 cut(s) 96, 534
CseI GACGC 1 cut(s) 332
CviAII CATG 3 cut(s) 21, 652, 857
DriI GACNNNNNGTC 1 cut(s) 1087
EaeI YGGCCR 2 cut(s) 47, 648
Eam1104I CTCTTC 3 cut(s) 45, 273, 441
Eam1105I GACNNNNNGTC 1 cut(s) 1087
EarI CTCTTC 3 cut(s) 45, 273, 441
EciI GGCGGA 1 cut(s) 950
Ecl136II GAGCTC 1 cut(s) 455
Eco130I CCWWGG 2 cut(s) 338, 856
Eco24I GRGCYC 3 cut(s) 87, 155, 457
Eco53kI GAGCTC 1 cut(s) 455
Eco57I CTGAAG 2 cut(s) 370, 737
Eco81I CCTNAGG 1 cut(s) 933
Eco88I CYCGRG 2 cut(s) 86, 154
EcoICRI GAGCTC 1 cut(s) 455
EcoNI CCTNNNNNAGG 1 cut(s) 821
EcoO109I RGGNCCY 2 cut(s) 96, 534
EcoRI GAATTC 1 cut(s) 1075
EcoT14I CCWWGG 2 cut(s) 338, 856
EcoT22I ATGCAT 1 cut(s) 402
EcoT38I GRGCYC 3 cut(s) 87, 155, 457
ErhI CCWWGG 2 cut(s) 338, 856
FaeI CATG 3 cut(s) 24, 655, 860
FalI AAGNNNNNCTT 4 cut(s) 331, 363, 872, 904
FatI CATG 3 cut(s) 20, 651, 856
FblI GTMKAC 1 cut(s) 1104
Fnu4HI GCNGC 7 cut(s) 219, 222, 225, 626, 629, 678, 955
FokI GGATG 2 cut(s) 111, 334
FriOI GRGCYC 3 cut(s) 87, 155, 457
Fsp4HI GCNGC 7 cut(s) 219, 222, 225, 626, 629, 678, 955
FspBI CTAG 2 cut(s) 240, 296
GlaI GCGC 1 cut(s) 377
GluI GCNGC 7 cut(s) 219, 222, 225, 626, 629, 678, 955
GsaI CCCAGC 1 cut(s) 481
GsuI CTGGAG 1 cut(s) 716
HaeIII GGCC 5 cut(s) 49, 97, 536, 650, 924
HgaI GACGC 1 cut(s) 332
HhaI GCGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 343
Hin1II CATG 3 cut(s) 24, 655, 860
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 1115
HinfI GANTC 9 cut(s) 4, 68, 77, 182, 355, 503, 831, 847, 1146
HphI GGTGA 2 cut(s) 158, 592
Hpy166II GTNNAC 2 cut(s) 772, 1105
Hpy188I TCNGA 9 cut(s) 9, 82, 352, 389, 609, 717, 1081, 1093, 1154
Hpy188III TCNNGA 5 cut(s) 368, 688, 763, 865, 889
Hpy8I GTNNAC 2 cut(s) 772, 1105
Hpy99I CGWCG 2 cut(s) 48, 1085
HpyAV CCTTC 8 cut(s) 138, 261, 357, 517, 526, 649, 712, 914
HpyCH4III ACNGT 1 cut(s) 1112
HpyCH4IV ACGT 2 cut(s) 781, 928
HpyCH4V TGCA 6 cut(s) 400, 496, 625, 810, 984, 1163
HpyF10VI GCNNNNNNNGC 7 cut(s) 110, 218, 224, 452, 960, 1123, 1169
HpySE526I ACGT 2 cut(s) 781, 928
Hsp92I GRCGYC 1 cut(s) 343
Hsp92II CATG 3 cut(s) 24, 655, 860
HspAI GCGC 1 cut(s) 376
LguI GCTCTTC 1 cut(s) 441
LmnI GCTCC 1 cut(s) 951
Lsp1109I GCAGC 6 cut(s) 205, 208, 211, 637, 640, 664
LweI GCATC 2 cut(s) 237, 575
MaeI CTAG 2 cut(s) 240, 296
MaeII ACGT 2 cut(s) 781, 928
MaeIII GTNAC 1 cut(s) 327
MboII GAAGA 7 cut(s) 27, 32, 260, 356, 376, 428, 769
MfeI CAATTG 1 cut(s) 1158
MflI RGATCY 4 cut(s) 32, 53, 381, 684
MhlI GDGCHC 3 cut(s) 87, 155, 457
MlsI TGGCCA 1 cut(s) 650
MluCI AATT 7 cut(s) 315, 392, 464, 875, 968, 1075, 1158
MluNI TGGCCA 1 cut(s) 650
MlyI GAGTC 3 cut(s) 13, 349, 512
MmeI TCCRAC 2 cut(s) 1104, 1168
Mox20I TGGCCA 1 cut(s) 650
Mph1103I ATGCAT 1 cut(s) 402
MscI TGGCCA 1 cut(s) 650
MseI TTAA 2 cut(s) 429, 555
MslI CAYNNNNRTG 1 cut(s) 242
Msp20I TGGCCA 1 cut(s) 650
MspA1I CMGCKG 1 cut(s) 631
MspCI CTTAAG 1 cut(s) 428
MunI CAATTG 1 cut(s) 1158
MwoI GCNNNNNNNGC 7 cut(s) 110, 218, 224, 452, 960, 1123, 1169
NcoI CCATGG 1 cut(s) 856
NlaIII CATG 3 cut(s) 24, 655, 860
NlaIV GGNNCC 3 cut(s) 98, 953, 1059
NmeAIII GCCGAG 1 cut(s) 706
NsiI ATGCAT 1 cut(s) 402
NspV TTCGAA 1 cut(s) 1073
OliI CACNNNNGTG 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 441
PfeI GAWTC 6 cut(s) 68, 77, 182, 831, 847, 1146
PflFI GACNNNGTC 1 cut(s) 21
PflMI CCANNNNNTGG 1 cut(s) 694
PkrI GCNGC 7 cut(s) 220, 223, 226, 627, 630, 679, 956
PleI GAGTC 3 cut(s) 12, 349, 511
PpsI GAGTC 3 cut(s) 12, 349, 511
PsiI TTATAA 1 cut(s) 993
Psp124BI GAGCTC 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 477
PspN4I GGNNCC 3 cut(s) 98, 953, 1059
PspPI GGNCC 2 cut(s) 96, 534
PstI CTGCAG 1 cut(s) 627
PstNI CAGNNNCTG 2 cut(s) 631, 694
PsuI RGATCY 4 cut(s) 32, 53, 381, 684
PsyI GACNNNGTC 1 cut(s) 21
PvuII CAGCTG 1 cut(s) 631
RseI CAYNNNNRTG 1 cut(s) 242
SacI GAGCTC 1 cut(s) 457
SapI GCTCTTC 1 cut(s) 441
SaqAI TTAA 2 cut(s) 429, 555
SatI GCNGC 7 cut(s) 219, 222, 225, 626, 629, 678, 955
Sau96I GGNCC 2 cut(s) 96, 534
SchI GAGTC 3 cut(s) 13, 349, 512
SduI GDGCHC 3 cut(s) 87, 155, 457
SfaNI GCATC 2 cut(s) 237, 575
SfcI CTRYAG 1 cut(s) 623
SfuI TTCGAA 1 cut(s) 1073
SmiMI CAYNNNNRTG 1 cut(s) 242
SmlI CTYRAG 3 cut(s) 428, 538, 739
SmoI CTYRAG 3 cut(s) 428, 538, 739
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 7 cut(s) 315, 392, 464, 875, 968, 1075, 1158
SsiI CCGC 2 cut(s) 955, 961
SspMI CTAG 2 cut(s) 240, 296
SstI GAGCTC 1 cut(s) 457
StyI CCWWGG 2 cut(s) 338, 856
TaaI ACNGT 1 cut(s) 1112
TaiI ACGT 2 cut(s) 784, 931
TaqI TCGA 3 cut(s) 762, 834, 1073
TasI AATT 7 cut(s) 315, 392, 464, 875, 968, 1075, 1158
TauI GCSGC 1 cut(s) 957
TfiI GAWTC 6 cut(s) 68, 77, 182, 831, 847, 1146
Tru1I TTAA 2 cut(s) 429, 555
Tru9I TTAA 2 cut(s) 429, 555
TscAI CASTG 2 cut(s) 220, 466
TseI GCWGC 6 cut(s) 218, 221, 224, 625, 628, 677
TspDTI ATGAA 3 cut(s) 338, 499, 668
TspRI CASTG 2 cut(s) 220, 466
Tth111I GACNNNGTC 1 cut(s) 21
Van91I CCANNNNNTGG 1 cut(s) 694
Vha464I CTTAAG 1 cut(s) 428
XagI CCTNNNNNAGG 1 cut(s) 821
XapI RAATTY 3 cut(s) 392, 968, 1075
XmiI GTMKAC 1 cut(s) 1104
XspI CTAG 2 cut(s) 240, 296
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.