pycom14g02970

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016366
Physical Location & Seq
Forward (+)
2282520 .. 2283549
1030 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 861 bp
ATGATGAGCGGTTCTTCCTCACTAGTTCCGGTTCCGAATCCCAAAAGGCTAGAAGGCAAAGTCGCAATTATTACTGGAGGTGCTAGTGGAATTGGAGAGAGCACTGCAAGATTGTTTGTCTTCCATGGTGCAAAAGTCGTCATAGCCGACGTCCAAGATGAACTAGCTCTGTCCCTCTGCAAAGAACTCAACTCAGACGGAGAGTCCATTTCGTACATACGTTGCGATGTCACTATCGATTTGGATGTGAAAAATGTTGTAGACGTGGCTCTGTCAAAGTACGGAAAGCTTGACATCATGTACAACAATGCAGGCATATCCGGCAAATTGGACCCATCGATTTTGGGAGCTGATGCCGAGAACTTCAAGCAGGTGTTTGATGTGAACGTCTACGGAGCTTTCTTGGGCGCCAAGCATGCTGCTAGGGCAATGATCGCTGCTAAAAAAGGTGTCATTCTTTTCACTTCGAGCGTGGCATCAGCTAGTTGCGGCGAGTCTACACACGCGTACACCATGTCAAAGCACGCGGTGGTGGGACTTATGAAGAGCTTGTGTGTGGAGTTGGGGCAGCATGGGATTAGAGTCAATTGCATATCTCCGTGTGCCATGGCAACCCCGTTGCTGACAAATCTTATGGGGATTGAGAAAAATACGGTCGAGAAGTTGATCTGCGCTTCTGCGGTGTTGAAAGAAGCGGTGCCGAGGGCAGAGGATGTGGCGGAGGCTGCCGTGTACTTGGCGAGTGAGGAGTCTAAATTTGTGAACGGACTGAACCTTCTTGTGGATGGAGGTTATAGCACCACTAATCAGTCTCTTAGTATGGTTTTGAGGAATTTAATGTCCTCAAGCCAGGCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

287

Amino Acids

30.05

Weight (kDa)

5.98

Isoelectric Point (pI)

25.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 20 - 210 1.9e-51 short chain dehydrogenase
KR PF08659 23 - 183 5.7e-11 KR domain
adh_short_C2 PF13561 28 - 266 1.1e-62 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016366)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19750
malus_domestica MD12G1032700.v1.1 MD14G1033600.v1.1
prunus_persica Prupe.7G099400_v2.0.a1
pyrus_communis pycom12g03020 pycom14g02970
rosa_chinensis RchiOBHm_Chr3g0475091
rosa_laevigata RLG00000023863
rosa_multiflora Rmu_sc0003093.1_g000023
rosa_roxburghii Rroxscaffold_6G00406570
rosa_rugosa Rorug03G0146700
rosa_samantha Rh3AG196500 Rh3BG226500 Rh3DG221900
rosa_wichuraiana Rw3G017880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 361
AatII GACGTC 1 cut(s) 153
Acc36I ACCTGC 1 cut(s) 361
AccB1I GGYRCC 2 cut(s) 407, 697
AccBSI CCGCTC 1 cut(s) 9
AccI GTMKAC 3 cut(s) 261, 390, 497
AccII CGCG 2 cut(s) 506, 527
AciI CCGC 6 cut(s) 9, 489, 527, 680, 695, 719
AcsI RAATTY 2 cut(s) 755, 832
AcyI GRCGYC 2 cut(s) 150, 408
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 5 cut(s) 215, 281, 302, 509, 734
AfiI CCNNNNNNNGG 1 cut(s) 781
AflIII ACRYGT 1 cut(s) 504
AgsI TTSAA 2 cut(s) 367, 688
AhdI GACNNNNNGTC 1 cut(s) 202
AhlI ACTAGT 1 cut(s) 22
AjiI CACGTC 1 cut(s) 265
AjnI CCWGG 1 cut(s) 849
AluBI AGCT 6 cut(s) 167, 289, 350, 398, 482, 549
AluI AGCT 6 cut(s) 167, 289, 350, 398, 482, 549
Alw21I GWGCWC 1 cut(s) 104
Alw26I GTCTC 1 cut(s) 816
ApeKI GCWGC 4 cut(s) 419, 437, 568, 725
ApoI RAATTY 2 cut(s) 755, 832
AspLEI GCGC 2 cut(s) 410, 674
AspS9I GGNCC 1 cut(s) 331
AvaII GGWCC 1 cut(s) 331
BanI GGYRCC 2 cut(s) 407, 697
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 4 cut(s) 406, 424, 580, 712
BccI CCATC 2 cut(s) 343, 779
BceAI ACGGC 1 cut(s) 713
BciT130I CCWGG 1 cut(s) 851
BcoDI GTCTC 1 cut(s) 816
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 6 cut(s) 23, 50, 84, 164, 423, 483
BfoI RGCGCY 1 cut(s) 411
BfuAI ACCTGC 1 cut(s) 361
BisI GCNGC 5 cut(s) 420, 438, 490, 569, 726
BlsI GCNGC 5 cut(s) 421, 439, 491, 570, 727
Bme1390I CCNGG 1 cut(s) 851
Bme18I GGWCC 1 cut(s) 331
BmeRI GACNNNNNGTC 1 cut(s) 202
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 4 cut(s) 33, 333, 409, 699
BmrFI CCNGG 1 cut(s) 851
BmsI GCATC 2 cut(s) 343, 485
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 1 cut(s) 829
Bsa29I ATCGAT 2 cut(s) 237, 338
BsaHI GRCGYC 2 cut(s) 150, 408
BsaJI CCNNGG 3 cut(s) 124, 606, 701
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 781
Bse1I ACTGG 1 cut(s) 79
Bse3DI GCAATG 1 cut(s) 435
BseBI CCWGG 1 cut(s) 851
BseCI ATCGAT 2 cut(s) 237, 338
BseDI CCNNGG 3 cut(s) 124, 606, 701
BseGI GGATG 3 cut(s) 250, 718, 790
BseLI CCNNNNNNNGG 1 cut(s) 781
BseMI GCAATG 1 cut(s) 435
BseMII CTCAG 1 cut(s) 207
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 761
BseXI GCAGC 4 cut(s) 406, 424, 580, 712
Bsh1236I CGCG 2 cut(s) 506, 527
Bsh1285I CGRYCG 1 cut(s) 657
BshNI GGYRCC 2 cut(s) 407, 697
BshVI ATCGAT 2 cut(s) 237, 338
BsiEI CGRYCG 1 cut(s) 657
BsiHKAI GWGCWC 1 cut(s) 104
BsiSI CCGG 2 cut(s) 29, 321
BslFI GGGAC 2 cut(s) 157, 549
BslI CCNNNNNNNGG 1 cut(s) 781
BsmAI GTCTC 1 cut(s) 816
BsmFI GGGAC 2 cut(s) 157, 549
Bsp1286I GDGCHC 1 cut(s) 104
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 2 cut(s) 432, 666
Bsp19I CCATGG 2 cut(s) 124, 606
BspACI CCGC 6 cut(s) 9, 489, 527, 680, 695, 719
BspCNI CTCAG 1 cut(s) 206
BspDI ATCGAT 2 cut(s) 237, 338
BspFNI CGCG 2 cut(s) 506, 527
BspLI GGNNCC 4 cut(s) 33, 333, 409, 699
BspMI ACCTGC 1 cut(s) 361
BspQI GCTCTTC 1 cut(s) 539
BspT107I GGYRCC 2 cut(s) 407, 697
BsrBI CCGCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 435
BsrGI TGTACA 1 cut(s) 300
BsrI ACTGG 1 cut(s) 79
BssECI CCNNGG 3 cut(s) 124, 606, 701
BssMI GATC 2 cut(s) 432, 666
BssNI GRCGYC 2 cut(s) 150, 408
BssT1I CCWWGG 2 cut(s) 124, 606
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 1 cut(s) 655
Bst6I CTCTTC 1 cut(s) 539
BstACI GRCGYC 2 cut(s) 150, 408
BstAUI TGTACA 1 cut(s) 300
BstC8I GCNNGC 3 cut(s) 313, 417, 525
BstDEI CTNAG 2 cut(s) 193, 815
BstDSI CCRYGG 2 cut(s) 124, 606
BstF5I GGATG 3 cut(s) 250, 718, 790
BstFNI CGCG 2 cut(s) 506, 527
BstH2I RGCGCY 1 cut(s) 411
BstHHI GCGC 2 cut(s) 410, 674
BstKTI GATC 2 cut(s) 435, 669
BstMAI GTCTC 1 cut(s) 816
BstMBI GATC 2 cut(s) 432, 666
BstMCI CGRYCG 1 cut(s) 657
BstMWI GCNNNNNNNGC 5 cut(s) 321, 416, 425, 434, 725
BstNI CCWGG 1 cut(s) 851
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 849
BstUI CGCG 2 cut(s) 506, 527
BstV1I GCAGC 4 cut(s) 406, 424, 580, 712
BstV2I GAAGAC 1 cut(s) 112
Bsu15I ATCGAT 2 cut(s) 237, 338
BsuTUI ATCGAT 2 cut(s) 237, 338
BtgI CCRYGG 2 cut(s) 124, 606
BtgZI GCGATG 1 cut(s) 240
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 3 cut(s) 250, 718, 790
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 1 cut(s) 102
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 3 cut(s) 313, 417, 525
CfoI GCGC 2 cut(s) 410, 674
Cfr13I GGNCC 1 cut(s) 331
ClaI ATCGAT 2 cut(s) 237, 338
Csp6I GTAC 5 cut(s) 214, 280, 301, 508, 733
CviAII CATG 6 cut(s) 125, 298, 416, 514, 572, 607
CviQI GTAC 5 cut(s) 214, 280, 301, 508, 733
DdeI CTNAG 2 cut(s) 193, 815
DinI GGCGCC 1 cut(s) 409
DpnI GATC 2 cut(s) 434, 668
DpnII GATC 2 cut(s) 432, 666
DraIII CACNNNGTG 1 cut(s) 529
DriI GACNNNNNGTC 1 cut(s) 202
Eam1104I CTCTTC 1 cut(s) 539
Eam1105I GACNNNNNGTC 1 cut(s) 202
EarI CTCTTC 1 cut(s) 539
EciI GGCGGA 1 cut(s) 734
Eco130I CCWWGG 2 cut(s) 124, 606
Eco47I GGWCC 1 cut(s) 331
EcoRII CCWGG 1 cut(s) 849
EcoT14I CCWWGG 2 cut(s) 124, 606
EgeI GGCGCC 1 cut(s) 409
EheI GGCGCC 1 cut(s) 409
ErhI CCWWGG 2 cut(s) 124, 606
FaeI CATG 6 cut(s) 128, 301, 419, 517, 575, 610
FalI AAGNNNNNCTT 1 cut(s) 838
FaqI GGGAC 2 cut(s) 157, 549
FatI CATG 6 cut(s) 124, 297, 415, 513, 571, 606
FblI GTMKAC 3 cut(s) 261, 390, 497
Fnu4HI GCNGC 5 cut(s) 420, 438, 490, 569, 726
FokI GGATG 3 cut(s) 257, 725, 797
Fsp4HI GCNGC 5 cut(s) 420, 438, 490, 569, 726
FspBI CTAG 6 cut(s) 23, 50, 84, 164, 423, 483
GlaI GCGC 2 cut(s) 409, 673
GluI GCNGC 5 cut(s) 420, 438, 490, 569, 726
GsuI CTGGAG 1 cut(s) 96
HaeII RGCGCY 1 cut(s) 411
HapII CCGG 2 cut(s) 29, 321
HhaI GCGC 2 cut(s) 410, 674
Hin1I GRCGYC 2 cut(s) 150, 408
Hin1II CATG 6 cut(s) 128, 301, 419, 517, 575, 610
Hin6I GCGC 2 cut(s) 408, 672
HinP1I GCGC 2 cut(s) 408, 672
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 5 cut(s) 37, 203, 494, 582, 749
HpaII CCGG 2 cut(s) 29, 321
Hpy166II GTNNAC 7 cut(s) 262, 385, 391, 498, 510, 733, 763
Hpy188I TCNGA 2 cut(s) 36, 196
Hpy188III TCNNGA 1 cut(s) 658
Hpy8I GTNNAC 7 cut(s) 262, 385, 391, 498, 510, 733, 763
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 2 cut(s) 47, 785
HpyCH4III ACNGT 1 cut(s) 655
HpyCH4IV ACGT 4 cut(s) 150, 220, 264, 387
HpyCH4V TGCA 5 cut(s) 107, 131, 180, 311, 591
HpyF10VI GCNNNNNNNGC 5 cut(s) 321, 416, 425, 434, 725
HpyF3I CTNAG 2 cut(s) 193, 815
HpySE526I ACGT 4 cut(s) 150, 220, 264, 387
Hsp92I GRCGYC 2 cut(s) 150, 408
Hsp92II CATG 6 cut(s) 128, 301, 419, 517, 575, 610
HspAI GCGC 2 cut(s) 408, 672
KasI GGCGCC 1 cut(s) 407
Kzo9I GATC 2 cut(s) 432, 666
LguI GCTCTTC 1 cut(s) 539
LmnI GCTCC 2 cut(s) 347, 395
LpnPI CCDG 6 cut(s) 42, 60, 297, 334, 356, 836
Lsp1109I GCAGC 4 cut(s) 406, 424, 580, 712
LweI GCATC 2 cut(s) 343, 485
MaeI CTAG 6 cut(s) 23, 50, 84, 164, 423, 483
MaeII ACGT 4 cut(s) 150, 220, 264, 387
MaeIII GTNAC 1 cut(s) 229
MalI GATC 2 cut(s) 434, 668
MbiI CCGCTC 1 cut(s) 9
MboI GATC 2 cut(s) 432, 666
MboII GAAGA 3 cut(s) 6, 112, 556
MfeI CAATTG 1 cut(s) 586
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 6 cut(s) 66, 90, 326, 586, 755, 832
MluI ACGCGT 1 cut(s) 504
Mly113I GGCGCC 1 cut(s) 408
MlyI GAGTC 4 cut(s) 212, 503, 591, 758
MseI TTAA 1 cut(s) 836
MspI CCGG 2 cut(s) 29, 321
MspR9I CCNGG 1 cut(s) 851
MunI CAATTG 1 cut(s) 586
MvaI CCWGG 1 cut(s) 851
MvnI CGCG 2 cut(s) 506, 527
MwoI GCNNNNNNNGC 5 cut(s) 321, 416, 425, 434, 725
NarI GGCGCC 1 cut(s) 408
NcoI CCATGG 2 cut(s) 124, 606
NdeII GATC 2 cut(s) 432, 666
NlaIII CATG 6 cut(s) 128, 301, 419, 517, 575, 610
NlaIV GGNNCC 4 cut(s) 33, 333, 409, 699
NmeAIII GCCGAG 2 cut(s) 382, 726
NmuCI GTSAC 1 cut(s) 229
NspI RCATGY 1 cut(s) 419
PaeI GCATGC 1 cut(s) 419
PaqCI CACCTGC 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PfeI GAWTC 1 cut(s) 37
PkrI GCNGC 5 cut(s) 421, 439, 491, 570, 727
PleI GAGTC 4 cut(s) 211, 502, 590, 757
PluTI GGCGCC 1 cut(s) 411
PpsI GAGTC 4 cut(s) 211, 502, 590, 757
Psp6I CCWGG 1 cut(s) 849
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 4 cut(s) 33, 333, 409, 699
PspPI GGNCC 1 cut(s) 331
RsaI GTAC 5 cut(s) 215, 281, 302, 509, 734
RsaNI GTAC 5 cut(s) 214, 280, 301, 508, 733
SapI GCTCTTC 1 cut(s) 539
SaqAI TTAA 1 cut(s) 836
SatI GCNGC 5 cut(s) 420, 438, 490, 569, 726
Sau3AI GATC 2 cut(s) 432, 666
Sau96I GGNCC 1 cut(s) 331
SchI GAGTC 4 cut(s) 212, 503, 591, 758
ScrFI CCNGG 1 cut(s) 851
SduI GDGCHC 1 cut(s) 104
SfaNI GCATC 2 cut(s) 343, 485
SfoI GGCGCC 1 cut(s) 409
SinI GGWCC 1 cut(s) 331
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
SpeI ACTAGT 1 cut(s) 22
SphI GCATGC 1 cut(s) 419
Sse9I AATT 6 cut(s) 66, 90, 326, 586, 755, 832
SsiI CCGC 6 cut(s) 9, 489, 527, 680, 695, 719
SspDI GGCGCC 1 cut(s) 407
SspMI CTAG 6 cut(s) 23, 50, 84, 164, 423, 483
StyD4I CCNGG 1 cut(s) 849
StyI CCWWGG 2 cut(s) 124, 606
TaaI ACNGT 1 cut(s) 655
TaiI ACGT 4 cut(s) 153, 223, 267, 390
TaqI TCGA 4 cut(s) 237, 338, 467, 657
TasI AATT 6 cut(s) 66, 90, 326, 586, 755, 832
TatI WGTACW 2 cut(s) 300, 732
TauI GCSGC 1 cut(s) 492
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 1 cut(s) 836
Tru9I TTAA 1 cut(s) 836
TscAI CASTG 1 cut(s) 109
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 4 cut(s) 419, 437, 568, 725
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 2 cut(s) 174, 557
TspGWI ACGGA 5 cut(s) 213, 297, 408, 588, 780
TspRI CASTG 1 cut(s) 109
VpaK11BI GGWCC 1 cut(s) 331
XapI RAATTY 2 cut(s) 755, 832
XceI RCATGY 1 cut(s) 419
XmiI GTMKAC 3 cut(s) 261, 390, 497
XspI CTAG 6 cut(s) 23, 50, 84, 164, 423, 483
ZraI GACGTC 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.