pycom14g03450

Trafficking protein particle complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010066
Physical Location & Seq
Forward (+)
2894978 .. 2907526
12549 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3864 bp
ATGGTGGATCCGGCGAATACGCCACTTGGCAGAATGCTATTGGAAGAGATCACTCCCGTTGTCATGGTGCTCCGTACGCCCCTCGTCGAAGAAGCCTGCCTCAAGAACGGCCTCACCTTAGTCCAAATGCTCAAGCCCTTCTGCGTCTTCAACAACATCGACGTGCCTGTGCGGACTGCGAGTGATCAACCCTACAGGCTTCAGAAGTTCGGATTGCGGTTGTTTTATGAGTTGGATATTCGGCAACCGAATTTGGAGGTAGCAAAAGAGAGGTTGAAACAAGTCATAAACCAGGCGGCGGATAAAGATCTTTCTGAATTGTGCTCAGACCTGCCTCAGATTGATAATGCATTATCCATATCGGAATCTGAAGTTCTACCATCATGGTTTCAGTTTTTCAACAAAGAGCTTGTACATAGCGTCTCTTTTTCAGACCATGAAGCTTTTGATCATCCTGTGGCATGTCTCGTTGTAGTTTCTTCAAAGGATGATCAGCCTATCAACAGATTTGTTGACCTCTTTAACACCAAGAAGTTGCCCTCCCTTCTTACTAATGGGGCAATGGACCCAAAGATTTTGAAGCATTATTTACTAGTGCATGATAAACAAGATGGCCCTTTAGAGAAGGCAACTAAAATTTTAACTGAAATGAGGAGCACTTTTGGCTCTGACTGTCAGTTACTGTGCATTAATTCTTCTCAAGATGGTGTGGTTGAACATCAAGATTATCCCTGGGTGCTTTATAAATTTGAAGATTTACCTAGTCAGCCGCTTCGTTGCTTCCTTAATATTGAGGATTTTAATGAGATAAAAGACCTGATGCAAGATTTATCAACTAAACACATTATTCCCTACATGGAGCAAAAAATACGTTTGCTCAATCAGCAGGTTGCTGCAACAAGGAAAGGTTTTAGGAACCAAATAAAAAATCTATGGTGGAGAAAAGGAAAAGATGATGTGGTGGATTCTCCCAGTGGCCCTACGTACACCTTTAGCTCCATTGAATCCCAAATTAGAGTTTTGGGTGATTATGCCTTCATGCTACGTGATTATGAACTTGCATTGTCGAATTATCGCCTAATATCCACAGATTACAAGCTTGACAAAGCCTGGAAACGCTATGCTGGCGTACAGGAAATGATGGGGCTTGCATATTTCATGTCAGACCAGTCAAGAAAGGATGCAGAGTATTGCATGGAAAACGCATTTACTACCTATTTAAAAGTTGCACCATCTAGCCAGCAAAACGCAACTAGGTGTGGTCTTTGGTGGGTGGAGATGTTGAAGGCACGACATCAGTATAAAGAGGCTGCAACTGTTTACTTCCGTGTTTGTACGGAGGAGCCACTGTATTCCGCTGTAATGCTTGAGCAAGCGTCTTATTGTTACTTGCTCTCTAGACCACCTATGTTGCACAAATATGGATTTCATCTTGTTCTTTCTGGTGACCGTTACAAGAAATCTGATCAGGTCAAACATGCAATTCGCACATATAGAGGTGCCATGTCTGTGTATACAGGAACTACGTGGAGCCATATCAAAGACCATGTTCATTTCCATATTGGACAGTGGTATGCTCTTCTTGGGCTATATGATTTGGCTGCAAATCATGTGATGGAGGTCCTGGCTTGTGGTCATCAGTCCAAGAAAACGCAGGAGTTATTCCTGAGAGACTTCCTTCAAATTGTTCAGAAAACAGGGAAAACATTTGAAGTATCAAAGCTTCAATTGCCGGAGATCAATATTTCATCTCTTAGGGTAATTTTTGAGGATCATCGGACCTATGCATCATCTGCAGCTGCAAGTGTCAAGGAAAGAATATGGCTCTCCTTAGAGGAGGAAATGATTCCAAATTTGTCCACCGCCAGAACTAATTGGTTGGAGTTACAATCAAAGCTTATCCCAAAGAAGTACAAAGAGTCAAATGTTTGTGTTGCAGGAGAAGCTGTCAGGGTTGATATTGAATTAAAAAATCCGTTGCAAATTCCCCTCCCTTTGTCTAGTGTTTCCCTATTATGCGAGCTTTCTGCTGTCTCAGATGAAATGAAATCCGATGCAAGTAGCTCGTTAGTGGAGATTCAGGATGGAGAATCTACTAGTTTAATTCATAGGGATGTGAACTTTGAGAGTTCTTTATTTTCTTTGTCTGATGTGGACTTTTCATTAGCAGGAGGTGAAACAATTGTGGTGCAACTAACGGTTACTCCTAGAGTAGAAGGCATTTTACAGATTGTTGGTGTAAAGTGGAAATTGTCAGGCTCTGTTGTTGGCTTCCATAAATTTGACACTAACCCCATGAAGAAGATAAGCAGAAACCGAATACAGAAGGCGAAGCATCCTCATTGTGATAATTTAAAGTTTGTAGTTGTTAAGAGTGTACCAAAGCTTGAGGGTGTCATTCATCCACTACCTAAAAGGGCATATACAGGAGATTTACGGCATCTTGTGTTGGAGTTGAAGAACAAATCTGAATTTGCTGTGAAGAATTTGAAAATGAAGATTAGTCATCCTAGATTCTTGAATCTTGGAAGGCGAGAAAGTTTGAACTTGGAGTTTCCTGCTTGCTTAGAAAACAAAAGTTCTGATCAGTCTGCTGAACATGCTAACCTTAATGATGTTTCTCATGCCCTGTTTCTATTTCCTGAGGACACTATTATTCAAGGGGAAACACCATTATCGTGGCCTCTTTGGTTTCGGGCAGCTGTTCCTGGGAACATTTCCTTGTACATAACAATATATTATGAGATGAGTGATATTTCAAGCACCATGAGATTTCGCACTTTACGCATGCATTACAATTTGCAGGTATTGCCGTCATTGGATGTGTCATTCCTTATCAGTCCTTGTCCATCAAGATTGCAAGAGTTTCTTGTGCGCATGGATGTTGTCAACAAGACAAGCTCTGAAAGTTTCCAAATTCATCAGTTGTCATCTGTTGGGAACCAATGGGAAATCTCATTACTTCAACCCGTTGATGCCATCTTTCCTTCACAATCTTTAACACCTCATCAAGCATTGTCATGCTTCTTCAGGCTCAAGAGTCGTGGCAAGTCATCAACTTCTGAAGACGAGAAATCTTCCCATCCCCGAGTTCAGGGAACTCATTTGAGATTGGGTACTCAGGGCAATAATGGACCACGTTTTGATATAGCTAGCTCTCCTCTGGCAGATTTCCACCTCTCTGAAAGACTGTACCAGGAAGTTTTAAATAAGGGAGACACGAATCCGGTTGACTTCATATTGATTTCCCGGCAGAATGACATTAATCCTGAGGTTTCTGAGCCACCGCGTCTTTTTTCTCATCATGCATGTCACTGCAGTACTGCGACCACGAGTCCTATATCATGGCTAGTGGACGGACCTCGAACCTTATACCATAACTTCTCTGCTTCTTTTTGTGAAATAAATCTTAGCATGACTATATGCAACACATCGGATGTAGTTGCATCTGTACGCATAAACACCTCGGACTCCAGTACTAGTGATCATTTAAGTGATGCAACTCCAGTTCAACCTGCCACGTCTTCTGGCGACCAAGATGGATGGCATGACCTCTCGCCAGTGACTGACATCAAAGTGACTTCCGATGTCCTTGGATCCCGGGCCAGTAAGTCAATTCCCGTGGAGAGCGTCTCCCCATTTATTTGGTCCGGGTCAAGCTCTACTAGAGTCCATCTTGATCCCATGTCAAGAACTGAAATTCCTCTTCAAGTTTGTGTGTTCTCTCCCGGGACATACGATCTGTCGAGCTATGTTTTGCATTGGAATCTTTTACTTTCTAATGATCAAGAAAATCGGGATAGGTCGTCTTCTGGGACGTGCCAGGGATATCCGTATTACCTTACTGTACTCCAGTCGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TRAPPC-Trs85 TRAPPC9-Trs120

Protein Analysis

1288

Amino Acids

145.8

Weight (kDa)

6.28

Isoelectric Point (pI)

47.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAPPC-Trs85 PF12739 16 - 431 2.7e-86 ER-Golgi trafficking TRAPP I complex 85 kDa subunit
Ig_TPPC8_1st PF24545 605 - 758 6.5e-15 TPPC8 first Ig-like domain
Ig_TPPC8_2nd PF24544 810 - 928 9.5e-07 TPPC8 second Ig-like domain
Ig_TPPC8_C PF24542 1191 - 1250 2.9e-07 TPPC8 C-terminal Ig-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010066)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16280 AT5G16280
fragaria_vesca FvH4_6g20450 FvH4_6g20450 FvH4_6g20450 FvH4_6g20450
malus_domestica MD12G1041600.v1.1 MD14G1040700.v1.1
prunus_persica Prupe.7G093600_v2.0.a1
pyrus_communis pycom12g03630 pycom14g03450
rosa_chinensis RchiOBHm_Chr3g0476301
rosa_laevigata RLG00000023780
rosa_multiflora Rmu_sc0000114.1_g000012
rosa_roxburghii Rroxscaffold_6G00405410
rosa_rugosa Rorug03G0153400
rosa_samantha Rh3AG204600 Rh3BG236000 Rh3CG230700 Rh3DG230500
rosa_wichuraiana Rw3G018590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
Acc16I TGCGCA 1 cut(s) 2876
Acc36I ACCTGC 4 cut(s) 339, 877, 2794, 3523
AccB1I GGYRCC 1 cut(s) 1499
AccI GTMKAC 1 cut(s) 1514
AccII CGCG 1 cut(s) 3289
AciI CCGC 8 cut(s) 172, 217, 296, 299, 770, 1356, 1863, 3287
AclWI GGATC 6 cut(s) 2, 15, 1779, 3591, 3604, 3674
AcuI CTGAAG 4 cut(s) 185, 390, 3013, 3084
AfiI CCNNNNNNNGG 2 cut(s) 298, 3094
AhdI GACNNNNNGTC 1 cut(s) 3333
AhlI ACTAGT 3 cut(s) 592, 2096, 3479
AjiI CACGTC 3 cut(s) 163, 3522, 3819
AjnI CCWGG 7 cut(s) 291, 731, 1109, 1623, 2707, 3195, 3822
AloI GAACNNNNNNTCC 2 cut(s) 3685, 3717
Alw21I GWGCWC 3 cut(s) 72, 326, 659
Alw26I GTCTC 6 cut(s) 427, 470, 1665, 2038, 3210, 3637
AlwI GGATC 6 cut(s) 2, 15, 1779, 3591, 3604, 3674
AlwNI CAGNNNCTG 3 cut(s) 682, 2261, 3566
Ama87I CYCGRG 3 cut(s) 3087, 3600, 3728
AoxI GGCC 5 cut(s) 109, 613, 976, 2681, 3603
ApeKI GCWGC 6 cut(s) 893, 1310, 1601, 1796, 1799, 2699
AseI ATTAAT 2 cut(s) 690, 3264
Asp700I GAANNNNTTC 1 cut(s) 1845
AspLEI GCGC 1 cut(s) 2877
AspS9I GGNCC 9 cut(s) 565, 614, 977, 1621, 1779, 3134, 3359, 3603, 3648
AsuC2I CCSGG 6 cut(s) 3250, 3601, 3602, 3652, 3729, 3730
AsuHPI GGTGA 4 cut(s) 106, 1037, 1457, 2186
AsuNHI GCTAGC 1 cut(s) 3152
AvaI CYCGRG 3 cut(s) 3087, 3600, 3728
AvaII GGWCC 6 cut(s) 565, 1621, 1779, 3134, 3359, 3648
AxyI CCTNAGG 2 cut(s) 2643, 3270
BamHI GGATCC 2 cut(s) 7, 3596
BanI GGYRCC 1 cut(s) 1499
BarI GAAGNNNNNNTAC 4 cut(s) 1707, 1739, 2208, 2240
BauI CACGAG 1 cut(s) 3331
BbsI GAAGAC 4 cut(s) 139, 3072, 3516, 3801
Bbv12I GWGCWC 3 cut(s) 72, 326, 659
BbvI GCAGC 6 cut(s) 880, 1297, 1588, 1786, 1808, 2711
BceAI ACGGC 3 cut(s) 124, 2453, 2797
BcgI CGANNNNNNTGC 4 cut(s) 2009, 2043, 2046, 2080
BciT130I CCWGG 7 cut(s) 293, 733, 1111, 1625, 2709, 3197, 3824
BclI TGATCA 7 cut(s) 184, 448, 490, 1465, 2584, 3484, 3784
BcnI CCSGG 6 cut(s) 3250, 3601, 3602, 3652, 3729, 3730
BcoDI GTCTC 6 cut(s) 427, 470, 1665, 2038, 3210, 3637
BcuI ACTAGT 3 cut(s) 592, 2096, 3479
BfmI CTRYAG 3 cut(s) 193, 1794, 3316
BfuAI ACCTGC 4 cut(s) 339, 877, 2794, 3523
BglII AGATCT 1 cut(s) 307
BisI GCNGC 8 cut(s) 297, 770, 894, 1311, 1602, 1797, 1800, 2700
BlsI GCNGC 8 cut(s) 298, 771, 895, 1312, 1603, 1798, 1801, 2701
BmcAI AGTACT 2 cut(s) 3322, 3478
Bme18I GGWCC 6 cut(s) 565, 1621, 1779, 3134, 3359, 3648
BmeRI GACNNNNNGTC 1 cut(s) 3333
BmeT110I CYCGRG 3 cut(s) 3087, 3600, 3728
BmgBI CACGTC 3 cut(s) 163, 3522, 3819
BmgT120I GGNCC 9 cut(s) 565, 614, 977, 1621, 1779, 3134, 3359, 3603, 3648
BmiI GGNNCC 8 cut(s) 9, 567, 917, 1344, 1501, 1532, 2942, 3598
BmrI ACTGGG 1 cut(s) 966
BmsI GCATC 9 cut(s) 810, 1171, 1796, 2044, 2344, 2449, 2965, 3455, 3487
BmtI GCTAGC 1 cut(s) 3156
BmuI ACTGGG 1 cut(s) 966
BpiI GAAGAC 4 cut(s) 139, 3072, 3516, 3801
BplI GAGNNNNNCTC 2 cut(s) 3617, 3649
BpmI CTGGAG 3 cut(s) 3457, 3489, 3836
BpuEI CTTGAG 6 cut(s) 86, 116, 684, 1388, 2408, 3020
BpuMI CCSGG 6 cut(s) 3250, 3601, 3602, 3652, 3729, 3730
BsaAI YACGTR 3 cut(s) 984, 1046, 1527
BsaBI GATNNNNATC 1 cut(s) 306
BsaJI CCNNGG 9 cut(s) 731, 732, 2708, 3465, 3592, 3600, 3621, 3728, 3823
BsaWI WCCGGW 1 cut(s) 3226
BsaXI ACNNNNNCTCC 6 cut(s) 1334, 1364, 1932, 1962, 2188, 2218
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 3094
Bse1I ACTGG 7 cut(s) 972, 1168, 3474, 3506, 3560, 3606, 3853
Bse21I CCTNAGG 2 cut(s) 2643, 3270
Bse3DI GCAATG 1 cut(s) 567
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 7 cut(s) 293, 733, 1111, 1625, 2709, 3197, 3824
BseDI CCNNGG 9 cut(s) 731, 732, 2708, 3465, 3592, 3600, 3621, 3728, 3823
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 2 cut(s) 298, 3094
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 8 cut(s) 339, 350, 1658, 2049, 2634, 3134, 3261, 3270
BseNI ACTGG 7 cut(s) 972, 1168, 3474, 3506, 3560, 3606, 3853
BseRI GAGGAG 4 cut(s) 667, 1355, 1850, 3150
BseXI GCAGC 6 cut(s) 880, 1297, 1588, 1786, 1808, 2711
Bsh1236I CGCG 1 cut(s) 3289
BshFI GGCC 5 cut(s) 111, 615, 978, 2683, 3605
BshNI GGYRCC 1 cut(s) 1499
BsiHKAI GWGCWC 3 cut(s) 72, 326, 659
BsiHKCI CYCGRG 3 cut(s) 3087, 3600, 3728
BsiSI CCGG 7 cut(s) 11, 1733, 3227, 3250, 3601, 3651, 3729
BsiWI CGTACG 1 cut(s) 74
BslFI GGGAC 2 cut(s) 3745, 3829
BslI CCNNNNNNNGG 2 cut(s) 298, 3094
BsmAI GTCTC 6 cut(s) 427, 470, 1665, 2038, 3210, 3637
BsmBI CGTCTC 2 cut(s) 427, 3637
BsmFI GGGAC 2 cut(s) 3745, 3829
BsmI GAATGC 1 cut(s) 39
BsnI GGCC 5 cut(s) 111, 615, 978, 2683, 3605
BsoBI CYCGRG 3 cut(s) 3087, 3600, 3728
Bsp1286I GDGCHC 3 cut(s) 72, 326, 659
Bsp1407I TGTACA 2 cut(s) 412, 2724
BspACI CCGC 8 cut(s) 172, 217, 296, 299, 770, 1356, 1863, 3287
BspANI GGCC 5 cut(s) 111, 615, 978, 2683, 3605
BspCNI CTCAG 8 cut(s) 338, 349, 1659, 2048, 2635, 3133, 3262, 3271
BspFNI CGCG 1 cut(s) 3289
BspLI GGNNCC 8 cut(s) 9, 567, 917, 1344, 1501, 1532, 2942, 3598
BspMAI CTGCAG 2 cut(s) 1798, 3320
BspMI ACCTGC 4 cut(s) 339, 877, 2794, 3523
BspOI GCTAGC 1 cut(s) 3156
BspPI GGATC 6 cut(s) 2, 15, 1779, 3591, 3604, 3674
BspQI GCTCTTC 1 cut(s) 1584
BspT107I GGYRCC 1 cut(s) 1499
BsrDI GCAATG 1 cut(s) 567
BsrGI TGTACA 2 cut(s) 412, 2724
BsrI ACTGG 7 cut(s) 972, 1168, 3474, 3506, 3560, 3606, 3853
BssECI CCNNGG 9 cut(s) 731, 732, 2708, 3465, 3592, 3600, 3621, 3728, 3823
BssNAI GTATAC 1 cut(s) 1515
BssSI CACGAG 1 cut(s) 3331
BssT1I CCWWGG 1 cut(s) 3592
Bst1107I GTATAC 1 cut(s) 1515
Bst2BI CACGAG 1 cut(s) 3331
Bst2UI CCWGG 7 cut(s) 293, 733, 1111, 1625, 2709, 3197, 3824
Bst4CI ACNGT 9 cut(s) 674, 684, 1318, 1350, 1451, 1569, 2200, 3192, 3847
Bst6I CTCTTC 3 cut(s) 39, 1584, 3711
BstAPI GCANNNNNTGC 2 cut(s) 1793, 2809
BstAUI TGTACA 2 cut(s) 412, 2724
BstBAI YACGTR 3 cut(s) 984, 1046, 1527
BstC8I GCNNGC 9 cut(s) 97, 1126, 1149, 1241, 1374, 2021, 2563, 2789, 3154
BstDSI CCRYGG 1 cut(s) 3621
BstEII GGTNACC 1 cut(s) 1445
BstFNI CGCG 1 cut(s) 3289
BstHHI GCGC 1 cut(s) 2877
BstMAI GTCTC 6 cut(s) 427, 470, 1665, 2038, 3210, 3637
BstNI CCWGG 7 cut(s) 293, 733, 1111, 1625, 2709, 3197, 3824
BstNSI RCATGY 5 cut(s) 465, 1481, 2603, 2791, 3312
BstPI GGTNACC 1 cut(s) 1445
BstSFI CTRYAG 3 cut(s) 193, 1794, 3316
BstSNI TACGTA 1 cut(s) 984
BstUI CGCG 1 cut(s) 3289
BstV1I GCAGC 6 cut(s) 880, 1297, 1588, 1786, 1808, 2711
BstV2I GAAGAC 4 cut(s) 139, 3072, 3516, 3801
BstX2I RGATCY 3 cut(s) 7, 307, 3596
BstXI CCANNNNNNTGG 2 cut(s) 2679, 3645
BstYI RGATCY 3 cut(s) 7, 307, 3596
BstZ17I GTATAC 1 cut(s) 1515
Bsu36I CCTNAGG 2 cut(s) 2643, 3270
BsuRI GGCC 5 cut(s) 111, 615, 978, 2683, 3605
BtgI CCRYGG 1 cut(s) 3621
BtrI CACGTC 3 cut(s) 163, 3522, 3819
BtsI GCAGTG 1 cut(s) 3313
BtsIMutI CAGTG 5 cut(s) 979, 1346, 1574, 3313, 3567
BveI ACCTGC 4 cut(s) 339, 877, 2794, 3523
Cac8I GCNNGC 9 cut(s) 97, 1126, 1149, 1241, 1374, 2021, 2563, 2789, 3154
CaiI CAGNNNCTG 3 cut(s) 682, 2261, 3566
CfoI GCGC 1 cut(s) 2877
Cfr13I GGNCC 9 cut(s) 565, 614, 977, 1621, 1779, 3134, 3359, 3603, 3648
Cfr9I CCCGGG 2 cut(s) 3600, 3728
CseI GACGC 5 cut(s) 133, 409, 1365, 3278, 3619
CspCI CAANNNNNGTGG 6 cut(s) 1392, 1427, 3025, 3060, 3603, 3638
DraI TTTAAA 3 cut(s) 1221, 2355, 3207
DriI GACNNNNNGTC 1 cut(s) 3333
Eam1104I CTCTTC 3 cut(s) 39, 1584, 3711
Eam1105I GACNNNNNGTC 1 cut(s) 3333
EarI CTCTTC 3 cut(s) 39, 1584, 3711
EciI GGCGGA 1 cut(s) 314
Eco105I TACGTA 1 cut(s) 984
Eco130I CCWWGG 1 cut(s) 3592
Eco32I GATATC 1 cut(s) 3830
Eco47I GGWCC 6 cut(s) 565, 1621, 1779, 3134, 3359, 3648
Eco57I CTGAAG 4 cut(s) 185, 390, 3013, 3084
Eco81I CCTNAGG 2 cut(s) 2643, 3270
Eco88I CYCGRG 3 cut(s) 3087, 3600, 3728
Eco91I GGTNACC 1 cut(s) 1445
EcoO109I RGGNCCY 1 cut(s) 1621
EcoO65I GGTNACC 1 cut(s) 1445
EcoRII CCWGG 7 cut(s) 291, 731, 1109, 1623, 2707, 3195, 3822
EcoRV GATATC 1 cut(s) 3830
EcoT14I CCWWGG 1 cut(s) 3592
EcoT22I ATGCAT 4 cut(s) 352, 1789, 2793, 3310
ErhI CCWWGG 1 cut(s) 3592
Esp3I CGTCTC 2 cut(s) 427, 3637
FalI AAGNNNNNCTT 2 cut(s) 2853, 2885
FaqI GGGAC 2 cut(s) 3745, 3829
FbaI TGATCA 7 cut(s) 184, 448, 490, 1465, 2584, 3484, 3784
FblI GTMKAC 1 cut(s) 1514
Fnu4HI GCNGC 8 cut(s) 297, 770, 894, 1311, 1602, 1797, 1800, 2700
Fsp4HI GCNGC 8 cut(s) 297, 770, 894, 1311, 1602, 1797, 1800, 2700
FspAI RTGCGCAY 1 cut(s) 2876
FspI TGCGCA 1 cut(s) 2876
GlaI GCGC 1 cut(s) 2876
GluI GCNGC 8 cut(s) 297, 770, 894, 1311, 1602, 1797, 1800, 2700
GsuI CTGGAG 3 cut(s) 3457, 3489, 3836
HaeIII GGCC 5 cut(s) 111, 615, 978, 2683, 3605
HapII CCGG 7 cut(s) 11, 1733, 3227, 3250, 3601, 3651, 3729
HgaI GACGC 5 cut(s) 133, 409, 1365, 3278, 3619
HhaI GCGC 1 cut(s) 2877
Hin6I GCGC 1 cut(s) 2875
HinP1I GCGC 1 cut(s) 2875
HincII GTYRAC 3 cut(s) 514, 2890, 3232
HindII GTYRAC 3 cut(s) 514, 2890, 3232
HindIII AAGCTT 5 cut(s) 441, 1097, 1721, 1895, 2384
HpaII CCGG 7 cut(s) 11, 1733, 3227, 3250, 3601, 3651, 3729
HphI GGTGA 4 cut(s) 106, 1037, 1457, 2186
Hpy99I CGWCG 2 cut(s) 89, 164
HpyCH4III ACNGT 9 cut(s) 674, 684, 1318, 1350, 1451, 1569, 2200, 3192, 3847
HpyCH4IV ACGT 8 cut(s) 162, 871, 983, 1045, 1526, 3139, 3521, 3818
HpySE526I ACGT 8 cut(s) 162, 871, 983, 1045, 1526, 3139, 3521, 3818
HspAI GCGC 1 cut(s) 2875
Ksp22I TGATCA 7 cut(s) 184, 448, 490, 1465, 2584, 3484, 3784
LguI GCTCTTC 1 cut(s) 1584
LmnI GCTCC 6 cut(s) 75, 654, 859, 1001, 1342, 1530
Lsp1109I GCAGC 6 cut(s) 880, 1297, 1588, 1786, 1808, 2711
LweI GCATC 9 cut(s) 810, 1171, 1796, 2044, 2344, 2449, 2965, 3455, 3487
MaeII ACGT 8 cut(s) 162, 871, 983, 1045, 1526, 3139, 3521, 3818
MaeIII GTNAC 9 cut(s) 678, 1385, 1445, 1451, 1884, 2200, 3311, 3562, 3577
MfeI CAATTG 2 cut(s) 1727, 2181
MflI RGATCY 3 cut(s) 7, 307, 3596
MhlI GDGCHC 3 cut(s) 72, 326, 659
MlyI GAGTC 5 cut(s) 1928, 3049, 3343, 3464, 3678
MmeI TCCRAC 4 cut(s) 213, 1860, 2430, 3837
Mph1103I ATGCAT 4 cut(s) 352, 1789, 2793, 3310
MroXI GAANNNNTTC 1 cut(s) 1845
MslI CAYNNNNRTG 8 cut(s) 161, 1419, 1508, 2112, 2677, 3019, 3492, 3575
MspA1I CMGCKG 3 cut(s) 1358, 1799, 2702
MspI CCGG 7 cut(s) 11, 1733, 3227, 3250, 3601, 3651, 3729
MunI CAATTG 2 cut(s) 1727, 2181
Mva1269I GAATGC 1 cut(s) 39
MvaI CCWGG 7 cut(s) 293, 733, 1111, 1625, 2709, 3197, 3824
MvnI CGCG 1 cut(s) 3289
NciI CCSGG 6 cut(s) 3250, 3601, 3602, 3652, 3729, 3730
NheI GCTAGC 1 cut(s) 3152
NlaIV GGNNCC 8 cut(s) 9, 567, 917, 1344, 1501, 1532, 2942, 3598
NmuCI GTSAC 4 cut(s) 1445, 3311, 3562, 3577
NsbI TGCGCA 1 cut(s) 2876
NsiI ATGCAT 4 cut(s) 352, 1789, 2793, 3310
NspI RCATGY 5 cut(s) 465, 1481, 2603, 2791, 3312
PaeI GCATGC 1 cut(s) 2791
PasI CCCWGGG 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 1584
PctI GAATGC 1 cut(s) 39
PdmI GAANNNNTTC 1 cut(s) 1845
Pfl23II CGTACG 1 cut(s) 74
PkrI GCNGC 8 cut(s) 298, 771, 895, 1312, 1603, 1798, 1801, 2701
PleI GAGTC 5 cut(s) 1927, 3048, 3342, 3464, 3677
PpsI GAGTC 5 cut(s) 1927, 3048, 3342, 3464, 3677
Ppu21I YACGTR 3 cut(s) 984, 1046, 1527
PpuMI RGGWCCY 1 cut(s) 1621
PshBI ATTAAT 2 cut(s) 690, 3264
PsiI TTATAA 1 cut(s) 744
Psp5II RGGWCCY 1 cut(s) 1621
Psp6I CCWGG 7 cut(s) 291, 731, 1109, 1623, 2707, 3195, 3822
PspEI GGTNACC 1 cut(s) 1445
PspGI CCWGG 7 cut(s) 291, 731, 1109, 1623, 2707, 3195, 3822
PspLI CGTACG 1 cut(s) 74
PspN4I GGNNCC 8 cut(s) 9, 567, 917, 1344, 1501, 1532, 2942, 3598
PspPI GGNCC 9 cut(s) 565, 614, 977, 1621, 1779, 3134, 3359, 3603, 3648
PspPPI RGGWCCY 1 cut(s) 1621
PstI CTGCAG 2 cut(s) 1798, 3320
PstNI CAGNNNCTG 3 cut(s) 682, 2261, 3566
PsuI RGATCY 3 cut(s) 7, 307, 3596
PvuII CAGCTG 2 cut(s) 1799, 2702
RseI CAYNNNNRTG 8 cut(s) 161, 1419, 1508, 2112, 2677, 3019, 3492, 3575
SapI GCTCTTC 1 cut(s) 1584
SatI GCNGC 8 cut(s) 297, 770, 894, 1311, 1602, 1797, 1800, 2700
Sau96I GGNCC 9 cut(s) 565, 614, 977, 1621, 1779, 3134, 3359, 3603, 3648
ScaI AGTACT 2 cut(s) 3322, 3478
SchI GAGTC 5 cut(s) 1928, 3049, 3343, 3464, 3678
SduI GDGCHC 3 cut(s) 72, 326, 659
SfaNI GCATC 9 cut(s) 810, 1171, 1796, 2044, 2344, 2449, 2965, 3455, 3487
SfcI CTRYAG 3 cut(s) 193, 1794, 3316
SinI GGWCC 6 cut(s) 565, 1621, 1779, 3134, 3359, 3648
SmaI CCCGGG 2 cut(s) 3602, 3730
SmiMI CAYNNNNRTG 8 cut(s) 161, 1419, 1508, 2112, 2677, 3019, 3492, 3575
SmlI CTYRAG 6 cut(s) 101, 131, 699, 1367, 2387, 3035
SmoI CTYRAG 6 cut(s) 101, 131, 699, 1367, 2387, 3035
SnaBI TACGTA 1 cut(s) 984
SpeI ACTAGT 3 cut(s) 592, 2096, 3479
SphI GCATGC 1 cut(s) 2791
SsiI CCGC 8 cut(s) 172, 217, 296, 299, 770, 1356, 1863, 3287
SspI AATATT 2 cut(s) 790, 1744
StyI CCWWGG 1 cut(s) 3592
TaaI ACNGT 9 cut(s) 674, 684, 1318, 1350, 1451, 1569, 2200, 3192, 3847
TaiI ACGT 8 cut(s) 165, 874, 986, 1048, 1529, 3142, 3524, 3821
TaqI TCGA 5 cut(s) 87, 159, 1067, 3364, 3746
TatI WGTACW 6 cut(s) 412, 1911, 2724, 3320, 3476, 3847
TauI GCSGC 2 cut(s) 299, 772
TscAI CASTG 5 cut(s) 979, 1353, 1574, 3320, 3567
TseFI GTSAC 4 cut(s) 1445, 3311, 3562, 3577
TseI GCWGC 6 cut(s) 893, 1310, 1601, 1796, 1799, 2699
Tsp45I GTSAC 4 cut(s) 1445, 3311, 3562, 3577
TspGWI ACGGA 6 cut(s) 62, 1316, 1352, 1965, 3372, 3822
TspMI CCCGGG 2 cut(s) 3600, 3728
TspRI CASTG 5 cut(s) 979, 1353, 1574, 3320, 3567
VpaK11BI GGWCC 6 cut(s) 565, 1621, 1779, 3134, 3359, 3648
VspI ATTAAT 2 cut(s) 690, 3264
XbaI TCTAGA 1 cut(s) 1397
XceI RCATGY 5 cut(s) 465, 1481, 2603, 2791, 3312
XmaI CCCGGG 2 cut(s) 3600, 3728
XmiI GTMKAC 1 cut(s) 1514
XmnI GAANNNNTTC 1 cut(s) 1845
ZrmI AGTACT 2 cut(s) 3322, 3478
Zsp2I ATGCAT 4 cut(s) 352, 1789, 2793, 3310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.