pycom14g05170
RLK Family

cysteine-rich RLK (RECEPTOR-like protein kinase) 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0018704
Physical Location & Seq
Reverse (-)
4962861 .. 4964240
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGGCAAATGGCACCACTGCACCTATTTTTGGGGCCGGCATTGTGGACCTCACACCGGTCCTCTCCCTCCATCATTGCTTGCTTGTTCCATCATTGTCTCATAATTTGTTATCTATTCCTCAAGTTACTGAGCAATTAGATTATGTTGTACTCACGTATCCACTATTTTGTTTACTTCAGGATATTCAGACCAATGAAACAATTGGGCGTAGCACTAAGAAAGTGGGGTTATATTATGTGGATGACATCGCTCCTGGCAGAGCTAATGCAGTTCGAGCATCTCGTACCAATAATTTACAAGAAGTTTGGTTATTGCATCATCCCCAAGAGGAGAGCACTAATAGGGATCAGGATGATCTTCGATGGTTGGAAATATTAGACAATTGGTTTTGGCAAGAAACCACTGAGAATGAGATACCTGGGTCTCGACAAGAAACTGCCGAAAATGAGATTAATGGGTCTCAGCAAGAAACTGTCGAGTATGTGATAGAGTTTCAGCTGCTACTTGCTAAGATAGACACAGCAGATCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 Retrotran_gag_2 Retrotran_gag_3 Retrotrans_gag gag_pre-integrs rve

Protein Analysis

178

Amino Acids

20.1

Weight (kDa)

4.49

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018704)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G03940
malus_domestica MD17G1120000.v1.1
pyrus_communis pycom03g19420 pycom10g09460 pycom10g21720 pycom13g28160 pycom14g05170
rosa_chinensis RchiOBHm_Chr3g0491631
rosa_wichuraiana Rw5G011130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 11
AclWI GGATC 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 161
AfaI GTAC 2 cut(s) 150, 286
AfiI CCNNNNNNNGG 2 cut(s) 29, 55
AgeI ACCGGT 1 cut(s) 55
AjnI CCWGG 2 cut(s) 253, 418
AluBI AGCT 2 cut(s) 263, 499
AluI AGCT 2 cut(s) 263, 499
Alw21I GWGCWC 1 cut(s) 338
Alw26I GTCTC 3 cut(s) 102, 429, 465
AlwI GGATC 1 cut(s) 354
AoxI GGCC 1 cut(s) 33
ApeKI GCWGC 1 cut(s) 499
AseI ATTAAT 1 cut(s) 453
AsiGI ACCGGT 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 33, 46, 58
AvaII GGWCC 2 cut(s) 46, 58
BaeI ACNNNNGTAYC 4 cut(s) 140, 173, 407, 440
BanI GGYRCC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 338
BbvI GCAGC 1 cut(s) 486
BccI CCATC 3 cut(s) 78, 97, 357
BciT130I CCWGG 2 cut(s) 255, 420
BciVI GTATCC 1 cut(s) 168
BcoDI GTCTC 3 cut(s) 102, 429, 465
BfuI GTATCC 1 cut(s) 168
BisI GCNGC 1 cut(s) 500
BlsI GCNGC 1 cut(s) 501
Bme1390I CCNGG 2 cut(s) 255, 420
Bme18I GGWCC 2 cut(s) 46, 58
BmgT120I GGNCC 3 cut(s) 33, 46, 58
BmiI GGNNCC 2 cut(s) 13, 34
BmrFI CCNGG 2 cut(s) 255, 420
BmsI GCATC 2 cut(s) 287, 325
BpuEI CTTGAG 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 156
BsaI GGTCTC 2 cut(s) 429, 465
BsaJI CCNNGG 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 55
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 55
Bse118I RCCGGY 2 cut(s) 35, 55
Bse3DI GCAATG 1 cut(s) 73
BseBI CCWGG 2 cut(s) 255, 420
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 3 cut(s) 247, 319, 358
BseLI CCNNNNNNNGG 2 cut(s) 29, 55
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 3 cut(s) 120, 396, 476
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 1 cut(s) 486
BshFI GGCC 1 cut(s) 35
BshNI GGYRCC 1 cut(s) 11
BshTI ACCGGT 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 338
BsiSI CCGG 2 cut(s) 36, 56
BslI CCNNNNNNNGG 2 cut(s) 29, 55
BsmAI GTCTC 3 cut(s) 102, 429, 465
BsnI GGCC 1 cut(s) 35
Bso31I GGTCTC 2 cut(s) 429, 465
Bsp1286I GDGCHC 1 cut(s) 338
Bsp143I GATC 3 cut(s) 346, 355, 526
BspANI GGCC 1 cut(s) 35
BspCNI CTCAG 3 cut(s) 121, 397, 475
BspLI GGNNCC 2 cut(s) 13, 34
BspPI GGATC 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 11
BspTNI GGTCTC 2 cut(s) 429, 465
BsrDI GCAATG 1 cut(s) 73
BsrFI RCCGGY 2 cut(s) 35, 55
BssAI RCCGGY 2 cut(s) 35, 55
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 3 cut(s) 346, 355, 526
Bst2UI CCWGG 2 cut(s) 255, 420
Bst4CI ACNGT 1 cut(s) 475
BstBAI YACGTR 1 cut(s) 156
BstC8I GCNNGC 2 cut(s) 37, 80
BstDEI CTNAG 5 cut(s) 129, 216, 405, 462, 510
BstF5I GGATG 3 cut(s) 247, 319, 358
BstKTI GATC 3 cut(s) 349, 358, 529
BstMAI GTCTC 3 cut(s) 102, 429, 465
BstMBI GATC 3 cut(s) 346, 355, 526
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNI CCWGG 2 cut(s) 255, 420
BstSCI CCNGG 2 cut(s) 253, 418
BstV1I GCAGC 1 cut(s) 486
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 1 cut(s) 35
BtgZI GCGATG 1 cut(s) 232
BtsCI GGATG 3 cut(s) 247, 319, 358
BtsI GCAGTG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 15, 402
Cac8I GCNNGC 2 cut(s) 37, 80
Cfr10I RCCGGY 2 cut(s) 35, 55
Cfr13I GGNCC 3 cut(s) 33, 46, 58
Csp6I GTAC 2 cut(s) 149, 285
CspAI ACCGGT 1 cut(s) 55
CviJI RGCY 3 cut(s) 35, 263, 499
CviKI_1 RGCY 3 cut(s) 35, 263, 499
CviQI GTAC 2 cut(s) 149, 285
DdeI CTNAG 5 cut(s) 129, 216, 405, 462, 510
DpnI GATC 3 cut(s) 348, 357, 528
DpnII GATC 3 cut(s) 346, 355, 526
Eco31I GGTCTC 2 cut(s) 429, 465
Eco47I GGWCC 2 cut(s) 46, 58
Eco57I CTGAAG 1 cut(s) 161
EcoRII CCWGG 2 cut(s) 253, 418
FaiI YATR 5 cut(s) 102, 144, 232, 237, 483
Fnu4HI GCNGC 1 cut(s) 500
FokI GGATG 3 cut(s) 254, 306, 365
Fsp4HI GCNGC 1 cut(s) 500
GluI GCNGC 1 cut(s) 500
HaeIII GGCC 1 cut(s) 35
HapII CCGG 2 cut(s) 36, 56
HpaII CCGG 2 cut(s) 36, 56
Hpy166II GTNNAC 2 cut(s) 46, 173
Hpy188I TCNGA 1 cut(s) 189
Hpy188III TCNNGA 3 cut(s) 179, 350, 426
Hpy8I GTNNAC 2 cut(s) 46, 173
HpyCH4III ACNGT 1 cut(s) 475
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 3 cut(s) 20, 269, 316
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 5 cut(s) 129, 216, 405, 462, 510
HpySE526I ACGT 1 cut(s) 155
KroI GCCGGC 1 cut(s) 35
KroNI GCCGGC 1 cut(s) 37
Kzo9I GATC 3 cut(s) 346, 355, 526
LmnI GCTCC 1 cut(s) 256
LpnPI CCDG 8 cut(s) 49, 69, 164, 240, 267, 335, 405, 432
Lsp1109I GCAGC 1 cut(s) 486
LweI GCATC 2 cut(s) 287, 325
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 1 cut(s) 124
MalI GATC 3 cut(s) 348, 357, 528
MboI GATC 3 cut(s) 346, 355, 526
MboII GAAGA 1 cut(s) 350
MfeI CAATTG 2 cut(s) 201, 382
MhlI GDGCHC 1 cut(s) 338
MluCI AATT 5 cut(s) 103, 134, 201, 292, 382
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 5 cut(s) 59, 71, 77, 129, 322
MroNI GCCGGC 1 cut(s) 35
MseI TTAA 1 cut(s) 453
MspA1I CMGCKG 1 cut(s) 499
MspI CCGG 2 cut(s) 36, 56
MspR9I CCNGG 2 cut(s) 255, 420
MunI CAATTG 2 cut(s) 201, 382
MvaI CCWGG 2 cut(s) 255, 420
MwoI GCNNNNNNNGC 1 cut(s) 275
NaeI GCCGGC 1 cut(s) 37
NdeII GATC 3 cut(s) 346, 355, 526
NgoMIV GCCGGC 1 cut(s) 35
NlaIV GGNNCC 2 cut(s) 13, 34
PcsI WCGNNNNNNNCGW 1 cut(s) 280
PdiI GCCGGC 1 cut(s) 37
PinAI ACCGGT 1 cut(s) 55
PkrI GCNGC 1 cut(s) 501
Ppu21I YACGTR 1 cut(s) 156
PshBI ATTAAT 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 253, 418
PspGI CCWGG 2 cut(s) 253, 418
PspN4I GGNNCC 2 cut(s) 13, 34
PspPI GGNCC 3 cut(s) 33, 46, 58
PvuII CAGCTG 1 cut(s) 499
RsaI GTAC 2 cut(s) 150, 286
RsaNI GTAC 2 cut(s) 149, 285
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 1 cut(s) 500
Sau3AI GATC 3 cut(s) 346, 355, 526
Sau96I GGNCC 3 cut(s) 33, 46, 58
ScrFI CCNGG 2 cut(s) 255, 420
SduI GDGCHC 1 cut(s) 338
SetI ASST 6 cut(s) 25, 51, 158, 265, 421, 501
SfaNI GCATC 2 cut(s) 287, 325
SinI GGWCC 2 cut(s) 46, 58
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
Sse9I AATT 5 cut(s) 103, 134, 201, 292, 382
SspI AATATT 1 cut(s) 375
StyD4I CCNGG 2 cut(s) 253, 418
TaaI ACNGT 1 cut(s) 475
TaiI ACGT 1 cut(s) 158
TaqI TCGA 4 cut(s) 274, 361, 427, 477
TasI AATT 5 cut(s) 103, 134, 201, 292, 382
TatI WGTACW 1 cut(s) 148
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 2 cut(s) 22, 409
TseI GCWGC 1 cut(s) 499
TspDTI ATGAA 1 cut(s) 210
TspRI CASTG 2 cut(s) 22, 409
VpaK11BI GGWCC 2 cut(s) 46, 58
VspI ATTAAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.