pycom14g09060

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012637
Physical Location & Seq
Reverse (-)
10590104 .. 10592855
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1140 bp
ATGGAGGAAAAGGAGAGCACCTTTTCTGGGTCACCCGGCAACTCGGAGACCGAGTCACTCCCAGTGACTCAGAAGGTGAGTATGCCCATGGCAGCCATGGCGGCCGTGGACGCCACCGTGAGCTCGGAGGAGAATTACAACAACACCCCTGTTTCGGAAACAACCCAAGTCGCAGAAGTTGCAGCAGCAGCAAGTGTTGGGCCTGGTGATTTGTCTGGGAAGAAGAAGAGAGGGAGGCCCAGAAAGTATGATGCAGATGGGAATTTGAGGCCGGGGTATAATAGTAATAAGAAAAGCAATAAGGGTGGCGCTGTCCCACCGCCACCGCCGGGGTTTTATCTTTCATCCCCTCTATCGTCTGAGTTTTCATTTTCTTCCAAAAGAGGAAGGGGGAAGCCTTCTGGATCCGGCAACTATCAGATTCTTGCTTCTTTGGGAGAGTTGTTTGCAAACACAGCTGGTGGGGACTTCACACCACATGTGGTTAATGTTAGCACTGGGGAGGATGTTGCAAGCAAAATATTTTCAATTTCTCACAAGGGTCGAGGCGTTTGCGTTCTCTCAGCAAATGGAGCTGTTTCTAATGTTACCATTCGCCAACCGGGTTCTTCTGGTGGCATTCTCACATATGAGGGACGCTTTGAGTTACTGTCTTTATCAGGGTCATTTACCGTCACTGAGATTGGTGGTGTAAGGAGTAGAACTGGTGGCTTGAGCGTCTCGTTGGCAGGCCCAGACGGTCGCGTAATAGGTGGTGGCATTGCCGGTGTGCTAACTGCTGCGAGCCCTATCCAAATTGTGGTTGGAAGCTTCATGCCAAATGGATACAAGGCACATAAAGTCAGGAAGCATTATCGTGAGAATACAGTAGGTTCCCCAATCTCAAGTGCTCCAGATACAGTCACTGCAGCAACTCCCGTCTCTCAAGCACAACCTGAAACTGAATCCAGACTGAATGCAATATCCCCTTTACCGGCACATAGCCACGGAGGAGAAGCAAATAAAAGCATGATCCAAATGCACGGCACCAGTGTTGTATCCACTAATGCCGGTTGGAATGGTGCAGATCTCAAGTCAGAACACAGGCCGTCCCCAGACATTAATTTATCCGTTCCTGGTGTATCTGTAACCGGTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

380

Amino Acids

38.85

Weight (kDa)

9.02

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 158 - 271 3.3e-22 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1025
AccB7I CCANNNNNTGG 1 cut(s) 799
AccII CGCG 1 cut(s) 744
AciI CCGC 3 cut(s) 101, 320, 326
AclWI GGATC 3 cut(s) 399, 412, 1006
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 1 cut(s) 262
AcyI GRCGYC 1 cut(s) 111
AfiI CCNNNNNNNGG 5 cut(s) 27, 154, 329, 799, 973
AflIII ACRYGT 1 cut(s) 478
AgeI ACCGGT 1 cut(s) 1130
AgsI TTSAA 1 cut(s) 528
AjnI CCWGG 2 cut(s) 202, 1114
AluBI AGCT 4 cut(s) 123, 458, 575, 810
AluI AGCT 4 cut(s) 123, 458, 575, 810
Alw21I GWGCWC 3 cut(s) 20, 125, 892
Alw26I GTCTC 3 cut(s) 41, 724, 925
AlwI GGATC 3 cut(s) 399, 412, 1006
AlwNI CAGNNNCTG 1 cut(s) 905
AoxI GGCC 6 cut(s) 102, 200, 236, 269, 730, 1085
ApeKI GCWGC 6 cut(s) 92, 182, 185, 188, 779, 908
ApoI RAATTY 1 cut(s) 262
AseI ATTAAT 1 cut(s) 1101
AsiGI ACCGGT 1 cut(s) 1130
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 3 cut(s) 200, 237, 731
AsuC2I CCSGG 4 cut(s) 36, 273, 330, 603
AsuHPI GGTGA 3 cut(s) 24, 88, 218
BamHI GGATCC 1 cut(s) 404
BanI GGYRCC 1 cut(s) 1025
BanII GRGCYC 2 cut(s) 125, 788
Bbv12I GWGCWC 3 cut(s) 20, 125, 892
BbvI GCAGC 6 cut(s) 104, 194, 197, 200, 766, 920
BccI CCATC 1 cut(s) 251
BceAI ACGGC 3 cut(s) 89, 1039, 1072
BcgI CGANNNNNNTGC 2 cut(s) 534, 568
BciT130I CCWGG 2 cut(s) 204, 1116
BciVI GTATCC 2 cut(s) 818, 1048
BcnI CCSGG 4 cut(s) 36, 273, 330, 603
BcoDI GTCTC 3 cut(s) 41, 724, 925
BfmI CTRYAG 1 cut(s) 906
BfoI RGCGCY 1 cut(s) 312
BfuI GTATCC 2 cut(s) 818, 1048
BglI GCCNNNNNGGC 1 cut(s) 101
BglII AGATCT 1 cut(s) 1066
BisI GCNGC 7 cut(s) 93, 102, 183, 186, 189, 780, 909
BlsI GCNGC 7 cut(s) 94, 103, 184, 187, 190, 781, 910
Bme1390I CCNGG 6 cut(s) 36, 204, 273, 330, 603, 1116
BmgT120I GGNCC 3 cut(s) 200, 237, 731
BmiI GGNNCC 3 cut(s) 406, 874, 1027
BmrFI CCNGG 6 cut(s) 36, 204, 273, 330, 603, 1116
BmrI ACTGGG 2 cut(s) 56, 507
BmsI GCATC 1 cut(s) 241
BmuI ACTGGG 2 cut(s) 56, 507
BpmI CTGGAG 1 cut(s) 876
BpuEI CTTGAG 4 cut(s) 733, 868, 909, 1055
BpuMI CCSGG 4 cut(s) 36, 273, 330, 603
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 6 cut(s) 87, 96, 105, 272, 329, 985
BsaWI WCCGGW 1 cut(s) 1130
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 5 cut(s) 27, 154, 329, 799, 973
Bse118I RCCGGY 4 cut(s) 764, 973, 1049, 1130
Bse1I ACTGG 4 cut(s) 62, 502, 709, 1029
Bse3DI GCAATG 1 cut(s) 759
BseBI CCWGG 2 cut(s) 204, 1116
BseDI CCNNGG 6 cut(s) 87, 96, 105, 272, 329, 985
BseGI GGATG 2 cut(s) 344, 511
BseLI CCNNNNNNNGG 5 cut(s) 27, 154, 329, 799, 973
BseMI GCAATG 1 cut(s) 759
BseMII CTCAG 4 cut(s) 83, 351, 576, 669
BseNI ACTGG 4 cut(s) 62, 502, 709, 1029
BseRI GAGGAG 2 cut(s) 143, 1005
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 6 cut(s) 104, 194, 197, 200, 766, 920
BsgI GTGCAG 1 cut(s) 1083
Bsh1236I CGCG 1 cut(s) 744
Bsh1285I CGRYCG 2 cut(s) 105, 742
BshFI GGCC 6 cut(s) 104, 202, 238, 271, 732, 1087
BshNI GGYRCC 1 cut(s) 1025
BshTI ACCGGT 1 cut(s) 1130
BsiEI CGRYCG 2 cut(s) 105, 742
BsiHKAI GWGCWC 3 cut(s) 20, 125, 892
BsiSI CCGG 9 cut(s) 36, 272, 329, 408, 602, 765, 974, 1050, 1131
BslFI GGGAC 4 cut(s) 299, 479, 648, 1075
BslI CCNNNNNNNGG 5 cut(s) 27, 154, 329, 799, 973
BsmAI GTCTC 3 cut(s) 41, 724, 925
BsmBI CGTCTC 2 cut(s) 724, 925
BsmFI GGGAC 4 cut(s) 299, 479, 648, 1075
BsmI GAATGC 2 cut(s) 618, 961
BsnI GGCC 6 cut(s) 104, 202, 238, 271, 732, 1087
Bso31I GGTCTC 1 cut(s) 41
Bsp1286I GDGCHC 4 cut(s) 20, 125, 788, 892
Bsp143I GATC 3 cut(s) 404, 1011, 1066
Bsp19I CCATGG 2 cut(s) 87, 96
BspACI CCGC 3 cut(s) 101, 320, 326
BspANI GGCC 6 cut(s) 104, 202, 238, 271, 732, 1087
BspCNI CTCAG 4 cut(s) 82, 352, 575, 670
BspFNI CGCG 1 cut(s) 744
BspLI GGNNCC 3 cut(s) 406, 874, 1027
BspMAI CTGCAG 1 cut(s) 910
BspPI GGATC 3 cut(s) 399, 412, 1006
BspT107I GGYRCC 1 cut(s) 1025
BspTNI GGTCTC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 759
BsrFI RCCGGY 4 cut(s) 764, 973, 1049, 1130
BsrI ACTGG 4 cut(s) 62, 502, 709, 1029
BssAI RCCGGY 4 cut(s) 764, 973, 1049, 1130
BssECI CCNNGG 6 cut(s) 87, 96, 105, 272, 329, 985
BssMI GATC 3 cut(s) 404, 1011, 1066
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 2 cut(s) 87, 96
Bst2UI CCWGG 2 cut(s) 204, 1116
Bst4CI ACNGT 6 cut(s) 118, 651, 673, 740, 868, 901
Bst6I CTCTTC 1 cut(s) 221
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 514, 730, 784
BstDEI CTNAG 5 cut(s) 69, 360, 562, 678, 1137
BstDSI CCRYGG 4 cut(s) 87, 96, 105, 985
BstEII GGTNACC 1 cut(s) 30
BstF5I GGATG 2 cut(s) 344, 511
BstFNI CGCG 1 cut(s) 744
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 3 cut(s) 407, 1014, 1069
BstMAI GTCTC 3 cut(s) 41, 724, 925
BstMBI GATC 3 cut(s) 404, 1011, 1066
BstMCI CGRYCG 2 cut(s) 105, 742
BstMWI GCNNNNNNNGC 7 cut(s) 98, 101, 110, 179, 188, 455, 572
BstNI CCWGG 2 cut(s) 204, 1116
BstNSI RCATGY 1 cut(s) 482
BstPI GGTNACC 1 cut(s) 30
BstSCI CCNGG 6 cut(s) 34, 202, 271, 328, 601, 1114
BstSFI CTRYAG 1 cut(s) 906
BstUI CGCG 1 cut(s) 744
BstV1I GCAGC 6 cut(s) 104, 194, 197, 200, 766, 920
BstX2I RGATCY 2 cut(s) 404, 1066
BstYI RGATCY 2 cut(s) 404, 1066
BstZI CGGCCG 1 cut(s) 102
BsuI GTATCC 2 cut(s) 818, 1048
BsuRI GGCC 6 cut(s) 104, 202, 238, 271, 732, 1087
BtgI CCRYGG 4 cut(s) 87, 96, 105, 985
BtsCI GGATG 2 cut(s) 344, 511
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 5 cut(s) 69, 495, 675, 903, 1036
Cac8I GCNNGC 3 cut(s) 514, 730, 784
CaiI CAGNNNCTG 1 cut(s) 905
CfoI GCGC 1 cut(s) 311
Cfr10I RCCGGY 4 cut(s) 764, 973, 1049, 1130
Cfr13I GGNCC 3 cut(s) 200, 237, 731
CseI GACGC 3 cut(s) 119, 645, 706
CspAI ACCGGT 1 cut(s) 1130
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 5 cut(s) 88, 97, 479, 814, 1009
DdeI CTNAG 5 cut(s) 69, 360, 562, 678, 1137
DpnI GATC 3 cut(s) 406, 1013, 1068
DpnII GATC 3 cut(s) 404, 1011, 1066
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Ecl136II GAGCTC 1 cut(s) 123
EclXI CGGCCG 1 cut(s) 102
Eco130I CCWWGG 2 cut(s) 87, 96
Eco24I GRGCYC 2 cut(s) 125, 788
Eco31I GGTCTC 1 cut(s) 41
Eco52I CGGCCG 1 cut(s) 102
Eco53kI GAGCTC 1 cut(s) 123
Eco91I GGTNACC 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 123
EcoO65I GGTNACC 1 cut(s) 30
EcoRII CCWGG 2 cut(s) 202, 1114
EcoT14I CCWWGG 2 cut(s) 87, 96
EcoT38I GRGCYC 2 cut(s) 125, 788
ErhI CCWWGG 2 cut(s) 87, 96
Esp3I CGTCTC 2 cut(s) 724, 925
FaeI CATG 5 cut(s) 91, 100, 482, 817, 1012
FaqI GGGAC 4 cut(s) 299, 479, 648, 1075
FatI CATG 5 cut(s) 87, 96, 478, 813, 1008
FauNDI CATATG 1 cut(s) 628
Fnu4HI GCNGC 7 cut(s) 93, 102, 183, 186, 189, 780, 909
FokI GGATG 2 cut(s) 331, 518
FriOI GRGCYC 2 cut(s) 125, 788
Fsp4HI GCNGC 7 cut(s) 93, 102, 183, 186, 189, 780, 909
GlaI GCGC 1 cut(s) 310
GluI GCNGC 7 cut(s) 93, 102, 183, 186, 189, 780, 909
GsuI CTGGAG 1 cut(s) 876
HaeII RGCGCY 1 cut(s) 312
HaeIII GGCC 6 cut(s) 104, 202, 238, 271, 732, 1087
HapII CCGG 9 cut(s) 36, 272, 329, 408, 602, 765, 974, 1050, 1131
HgaI GACGC 3 cut(s) 119, 645, 706
HhaI GCGC 1 cut(s) 311
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 5 cut(s) 91, 100, 482, 817, 1012
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HindIII AAGCTT 1 cut(s) 808
HinfI GANTC 4 cut(s) 53, 67, 421, 944
HpaII CCGG 9 cut(s) 36, 272, 329, 408, 602, 765, 974, 1050, 1131
HphI GGTGA 3 cut(s) 24, 88, 218
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 7 cut(s) 46, 72, 127, 157, 361, 420, 1078
Hpy188III TCNNGA 5 cut(s) 402, 844, 857, 893, 948
Hpy8I GTNNAC 1 cut(s) 109
HpyAV CCTTC 3 cut(s) 67, 381, 408
HpyCH4III ACNGT 6 cut(s) 118, 651, 673, 740, 868, 901
HpyCH4V TGCA 8 cut(s) 182, 254, 449, 512, 908, 959, 1021, 1064
HpyF10VI GCNNNNNNNGC 7 cut(s) 98, 101, 110, 179, 188, 455, 572
HpyF3I CTNAG 5 cut(s) 69, 360, 562, 678, 1137
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 5 cut(s) 91, 100, 482, 817, 1012
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 3 cut(s) 404, 1011, 1066
LmnI GCTCC 2 cut(s) 572, 895
Lsp1109I GCAGC 6 cut(s) 104, 194, 197, 200, 766, 920
LweI GCATC 1 cut(s) 241
MaeIII GTNAC 8 cut(s) 30, 54, 64, 586, 645, 673, 901, 1126
MalI GATC 3 cut(s) 406, 1013, 1068
MboI GATC 3 cut(s) 404, 1011, 1066
MboII GAAGA 5 cut(s) 232, 235, 238, 366, 600
MflI RGATCY 2 cut(s) 404, 1066
MhlI GDGCHC 4 cut(s) 20, 125, 788, 892
MluCI AATT 5 cut(s) 133, 262, 528, 795, 1102
MlyI GAGTC 2 cut(s) 61, 62
MmeI TCCRAC 2 cut(s) 784, 1034
MseI TTAA 2 cut(s) 486, 1101
MslI CAYNNNNRTG 1 cut(s) 855
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 9 cut(s) 36, 272, 329, 408, 602, 765, 974, 1050, 1131
MspR9I CCNGG 6 cut(s) 36, 204, 273, 330, 603, 1116
Mva1269I GAATGC 2 cut(s) 618, 961
MvaI CCWGG 2 cut(s) 204, 1116
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 7 cut(s) 98, 101, 110, 179, 188, 455, 572
NciI CCSGG 4 cut(s) 36, 273, 330, 603
NcoI CCATGG 2 cut(s) 87, 96
NdeI CATATG 1 cut(s) 628
NdeII GATC 3 cut(s) 404, 1011, 1066
NlaIII CATG 5 cut(s) 91, 100, 482, 817, 1012
NlaIV GGNNCC 3 cut(s) 406, 874, 1027
NmuCI GTSAC 5 cut(s) 30, 54, 64, 673, 901
NspI RCATGY 1 cut(s) 482
PciI ACATGT 1 cut(s) 478
PctI GAATGC 2 cut(s) 618, 961
PfeI GAWTC 2 cut(s) 421, 944
PflFI GACNNNGTC 1 cut(s) 52
PflMI CCANNNNNTGG 1 cut(s) 799
PinAI ACCGGT 1 cut(s) 1130
PkrI GCNGC 7 cut(s) 94, 103, 184, 187, 190, 781, 910
PleI GAGTC 2 cut(s) 61, 61
PpsI GAGTC 2 cut(s) 61, 61
PscI ACATGT 1 cut(s) 478
PshBI ATTAAT 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 202, 1114
PspEI GGTNACC 1 cut(s) 30
PspGI CCWGG 2 cut(s) 202, 1114
PspN4I GGNNCC 3 cut(s) 406, 874, 1027
PspPI GGNCC 3 cut(s) 200, 237, 731
PstI CTGCAG 1 cut(s) 910
PstNI CAGNNNCTG 1 cut(s) 905
PsuI RGATCY 2 cut(s) 404, 1066
PsyI GACNNNGTC 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 458
RseI CAYNNNNRTG 1 cut(s) 855
SacI GAGCTC 1 cut(s) 125
SaqAI TTAA 2 cut(s) 486, 1101
SatI GCNGC 7 cut(s) 93, 102, 183, 186, 189, 780, 909
Sau3AI GATC 3 cut(s) 404, 1011, 1066
Sau96I GGNCC 3 cut(s) 200, 237, 731
SchI GAGTC 2 cut(s) 61, 62
ScrFI CCNGG 6 cut(s) 36, 204, 273, 330, 603, 1116
SduI GDGCHC 4 cut(s) 20, 125, 788, 892
SetI ASST 9 cut(s) 23, 78, 125, 460, 577, 754, 812, 874, 937
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 906
SmiMI CAYNNNNRTG 1 cut(s) 855
SmlI CTYRAG 4 cut(s) 712, 883, 924, 1070
SmoI CTYRAG 4 cut(s) 712, 883, 924, 1070
Sse9I AATT 5 cut(s) 133, 262, 528, 795, 1102
SsiI CCGC 3 cut(s) 101, 320, 326
SspI AATATT 1 cut(s) 522
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 6 cut(s) 34, 202, 271, 328, 601, 1114
StyI CCWWGG 2 cut(s) 87, 96
TaaI ACNGT 6 cut(s) 118, 651, 673, 740, 868, 901
TaqI TCGA 1 cut(s) 544
TaqII GACCGA 1 cut(s) 65
TasI AATT 5 cut(s) 133, 262, 528, 795, 1102
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 2 cut(s) 421, 944
Tru1I TTAA 2 cut(s) 486, 1101
Tru9I TTAA 2 cut(s) 486, 1101
TscAI CASTG 5 cut(s) 69, 502, 682, 910, 1036
TseFI GTSAC 5 cut(s) 30, 54, 64, 673, 901
TseI GCWGC 6 cut(s) 92, 182, 185, 188, 779, 908
Tsp45I GTSAC 5 cut(s) 30, 54, 64, 673, 901
TspDTI ATGAA 3 cut(s) 333, 357, 802
TspGWI ACGGA 2 cut(s) 1002, 1099
TspRI CASTG 5 cut(s) 69, 502, 682, 910, 1036
Tth111I GACNNNGTC 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 799
VspI ATTAAT 1 cut(s) 1101
XapI RAATTY 1 cut(s) 262
XceI RCATGY 1 cut(s) 482
XcmI CCANNNNNNNNNTGG 3 cut(s) 94, 103, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.