pycom14g09140

3-deoxy-D-manno-octulosonic acid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008414
Physical Location & Seq
Forward (+)
10751961 .. 10756009
4049 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1329 bp
ATGGCGGCAACGAAGAGCAAGGCAGTCTACAATGTTTACAGAGCGCTGACCCATGGTCTATCACCATTGCTGTACCTTCACCTCCACTGGCGGAAACTCCGAGGCCTCGAGCACCCACTCCGATGGCGTGAGCGTCTGGGCCGACCTTCCCTCCCTCGCCCCGAAGGCCCACTCATCTGGTTCCACGCCGTCTCATTAGGTGAAGGAATGGCTGCGATTCCGGTGATCAAGCAATGCATTCAGCGGAGGCCTGATTTAACTATTCTGATGACCACTACGACGTCATCTGCGTTTGGAGTAATAGAGAAACGTCTTCCAACTGGTGTCTTACATCAGTTTGCGCCGGTCGATACACCTTCTGCTGTGGACTCATTCCTTGGTTACTGGAAGCCAAATGCGATTGTGCTTTTGGAGAGTGAACTATGGCCAAATTTAATTATGGCTGCTTCAGAAAAGGGTATCATGCTGGCATTGTTAAATGCTCGAGTATCTACTAAATCCTTTAAACGTTGGTCAGGACCAGCGCTTCTTCCTCTTATTTCATTGATGCTATCAAAGTTTTCATTGATCTCCCCATTGAGCAATATGCAGGCCATCCACTTTCAGCTGCTGCATGCCCCGCCTTTTGTGATCAACTTTTCTGGTGACTTGAAGTATGTGGTTGGAGAATTTGACATTTCTGAGGGAGAGATCAAAAGCATGGAAGATTTAAAAGTACACTGTGCCCACAGGAAGGTTTGGATGGCTTCTTCCATACATAAGGGGGAAGAAGAAGTAATACTTGGCATTCACAAAGTGCTGCGACAAGAGTACCCTGACTTGTTAACTATTATTGTGCCTCGACATCCACATCATGGAAAAGAGATTGCTCAAAAATTGTGGAAAGAAGGGCATGGTGTAGGGTTGAGGTCTCAACGTGATAAACTCTTTGGAGACACAAGTATATATGTGGTGGACACATTAGGTGAATTGAAACACCTTTATAAGTTATCCCCTGTAGCTGTAATTGGAGGTTCCTTCTTTCCTAGCCTTTCTGGCCATAACATATCAGAAGCTGCTGCAGCAGGCTGTGCAACTTTGACTGGTCCTTACATCGGGCATTTTTCAATCATGGTACTGGAAATGCAACGGTTAAATCCTCTGTCAGTTCTGCAGGTTGGGGGAAAATTAGAACTTGAAGAAGCTCTTAGAAAGCTGTTTGGTGATGTCAAAGTTCTAGAAGCACATTGTGCTGCAGCAAAACAAGTGTATCATACCCTATCAAGTGGTGTGGTTCAAAACGTTTGGAATCTATTAGATATTCAGATTCTCCAACGAGCTTTGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00540 ko01100 map00540 map01100
Pfam Domains
Protein Families
Glycos_transf_N

Protein Analysis

443

Amino Acids

49.16

Weight (kDa)

9.32

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_N PF04413 41 - 219 6.4e-54 3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 984
AatII GACGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 1144
AccB7I CCANNNNNTGG 1 cut(s) 854
AccI GTMKAC 1 cut(s) 27
AciI CCGC 4 cut(s) 5, 91, 244, 620
AclI AACGTT 2 cut(s) 508, 1281
AcoI YGGCCR 2 cut(s) 425, 1036
AcsI RAATTY 2 cut(s) 430, 668
AcuI CTGAAG 1 cut(s) 432
AcyI GRCGYC 1 cut(s) 281
AdeI CACNNNGTG 2 cut(s) 796, 1229
AfaI GTAC 4 cut(s) 74, 717, 812, 1116
AfeI AGCGCT 2 cut(s) 45, 525
AfiI CCNNNNNNNGG 3 cut(s) 733, 854, 1094
AgsI TTSAA 5 cut(s) 652, 973, 1107, 1178, 1277
AhdI GACNNNNNGTC 1 cut(s) 54
AjuI GAANNNNNNNTTGG 2 cut(s) 765, 797
AluBI AGCT 7 cut(s) 607, 1001, 1055, 1184, 1195, 1319, 1326
AluI AGCT 7 cut(s) 607, 1001, 1055, 1184, 1195, 1319, 1326
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 3 cut(s) 196, 915, 927
AlwNI CAGNNNCTG 2 cut(s) 610, 1055
Ama87I CYCGRG 2 cut(s) 107, 483
Aor51HI AGCGCT 2 cut(s) 45, 525
AoxI GGCC 7 cut(s) 103, 139, 166, 248, 425, 591, 1036
ApoI RAATTY 2 cut(s) 430, 668
AspLEI GCGC 3 cut(s) 46, 343, 526
AspS9I GGNCC 4 cut(s) 139, 167, 518, 1085
AsuHPI GGTGA 7 cut(s) 54, 71, 212, 235, 656, 977, 1214
AvaI CYCGRG 2 cut(s) 107, 483
AvaII GGWCC 2 cut(s) 518, 1085
BaeGI GKGCMC 1 cut(s) 727
BaeI ACNNNNGTAYC 4 cut(s) 794, 827, 1232, 1265
BalI TGGCCA 2 cut(s) 427, 1038
BarI GAAGNNNNNNTAC 2 cut(s) 762, 794
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 1 cut(s) 114
BccI CCATC 3 cut(s) 117, 602, 736
BceAI ACGGC 1 cut(s) 173
BclI TGATCA 2 cut(s) 225, 630
BcoDI GTCTC 3 cut(s) 196, 915, 927
BfaI CTAG 2 cut(s) 1026, 1217
BfmI CTRYAG 4 cut(s) 996, 1059, 1151, 1233
BfoI RGCGCY 2 cut(s) 47, 527
BfuAI ACCTGC 1 cut(s) 1144
BglI GCCNNNNNGGC 2 cut(s) 165, 1035
Bme18I GGWCC 2 cut(s) 518, 1085
BmeRI GACNNNNNGTC 1 cut(s) 54
BmeT110I CYCGRG 2 cut(s) 107, 483
BmgT120I GGNCC 4 cut(s) 139, 167, 518, 1085
BmiI GGNNCC 2 cut(s) 182, 1015
BmsI GCATC 1 cut(s) 537
BpiI GAAGAC 1 cut(s) 305
BsaHI GRCGYC 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 915
BsaJI CCNNGG 3 cut(s) 52, 100, 376
BsaWI WCCGGW 1 cut(s) 220
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 3 cut(s) 733, 854, 1094
Bse118I RCCGGY 1 cut(s) 343
Bse1I ACTGG 5 cut(s) 92, 325, 389, 1087, 1122
Bse3DI GCAATG 2 cut(s) 65, 239
BseDI CCNNGG 3 cut(s) 52, 100, 376
BseGI GGATG 3 cut(s) 594, 747, 844
BseLI CCNNNNNNNGG 3 cut(s) 733, 854, 1094
BseMI GCAATG 2 cut(s) 65, 239
BseMII CTCAG 1 cut(s) 672
BseNI ACTGG 5 cut(s) 92, 325, 389, 1087, 1122
BseSI GKGCMC 1 cut(s) 727
Bsh1285I CGRYCG 1 cut(s) 348
BshFI GGCC 7 cut(s) 105, 141, 168, 250, 427, 593, 1038
BsiEI CGRYCG 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 114
BsiHKCI CYCGRG 2 cut(s) 107, 483
BsiSI CCGG 2 cut(s) 221, 344
BslI CCNNNNNNNGG 3 cut(s) 733, 854, 1094
BsmAI GTCTC 3 cut(s) 196, 915, 927
BsmBI CGTCTC 1 cut(s) 196
BsmI GAATGC 2 cut(s) 237, 786
BsnI GGCC 7 cut(s) 105, 141, 168, 250, 427, 593, 1038
Bso31I GGTCTC 1 cut(s) 915
BsoBI CYCGRG 2 cut(s) 107, 483
Bsp1286I GDGCHC 2 cut(s) 114, 727
Bsp143I GATC 4 cut(s) 225, 567, 630, 690
Bsp19I CCATGG 1 cut(s) 52
BspACI CCGC 4 cut(s) 5, 91, 244, 620
BspANI GGCC 7 cut(s) 105, 141, 168, 250, 427, 593, 1038
BspCNI CTCAG 1 cut(s) 673
BspLI GGNNCC 2 cut(s) 182, 1015
BspMAI CTGCAG 3 cut(s) 1063, 1155, 1237
BspMI ACCTGC 1 cut(s) 1144
BspQI GCTCTTC 1 cut(s) 8
BspTNI GGTCTC 1 cut(s) 915
BsrDI GCAATG 2 cut(s) 65, 239
BsrFI RCCGGY 1 cut(s) 343
BsrI ACTGG 5 cut(s) 92, 325, 389, 1087, 1122
BssAI RCCGGY 1 cut(s) 343
BssECI CCNNGG 3 cut(s) 52, 100, 376
BssMI GATC 4 cut(s) 225, 567, 630, 690
BssNI GRCGYC 1 cut(s) 281
BssT1I CCWWGG 2 cut(s) 52, 376
Bst4CI ACNGT 2 cut(s) 722, 1131
Bst6I CTCTTC 1 cut(s) 8
BstACI GRCGYC 1 cut(s) 281
BstAPI GCANNNNNTGC 2 cut(s) 1070, 1229
BstC8I GCNNGC 4 cut(s) 468, 591, 615, 1066
BstDEI CTNAG 2 cut(s) 681, 1187
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 3 cut(s) 594, 747, 844
BstH2I RGCGCY 2 cut(s) 47, 527
BstHHI GCGC 3 cut(s) 46, 343, 526
BstKTI GATC 4 cut(s) 228, 570, 633, 693
BstMAI GTCTC 3 cut(s) 196, 915, 927
BstMBI GATC 4 cut(s) 225, 567, 630, 690
BstMCI CGRYCG 1 cut(s) 348
BstMWI GCNNNNNNNGC 6 cut(s) 165, 619, 1035, 1061, 1070, 1229
BstNSI RCATGY 1 cut(s) 617
BstSFI CTRYAG 4 cut(s) 996, 1059, 1151, 1233
BstSLI GKGCMC 1 cut(s) 727
BstV2I GAAGAC 1 cut(s) 305
BstXI CCANNNNNNTGG 2 cut(s) 123, 177
BsuRI GGCC 7 cut(s) 105, 141, 168, 250, 427, 593, 1038
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 3 cut(s) 594, 747, 844
BtsIMutI CAGTG 2 cut(s) 85, 718
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 4 cut(s) 468, 591, 615, 1066
CaiI CAGNNNCTG 2 cut(s) 610, 1055
CfoI GCGC 3 cut(s) 46, 343, 526
Cfr10I RCCGGY 1 cut(s) 343
Cfr13I GGNCC 4 cut(s) 139, 167, 518, 1085
CseI GACGC 1 cut(s) 122
Csp6I GTAC 4 cut(s) 73, 716, 811, 1115
CspCI CAANNNNNGTGG 4 cut(s) 860, 895, 1251, 1286
CviAII CATG 7 cut(s) 53, 463, 614, 700, 854, 893, 1111
CviQI GTAC 4 cut(s) 73, 716, 811, 1115
DdeI CTNAG 2 cut(s) 681, 1187
DpnI GATC 4 cut(s) 227, 569, 632, 692
DpnII GATC 4 cut(s) 225, 567, 630, 690
DraI TTTAAA 2 cut(s) 505, 711
DraIII CACNNNGTG 2 cut(s) 796, 1229
DriI GACNNNNNGTC 1 cut(s) 54
EaeI YGGCCR 2 cut(s) 425, 1036
Eam1104I CTCTTC 1 cut(s) 8
Eam1105I GACNNNNNGTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 1 cut(s) 106
Eco130I CCWWGG 2 cut(s) 52, 376
Eco147I AGGCCT 2 cut(s) 105, 250
Eco31I GGTCTC 1 cut(s) 915
Eco47I GGWCC 2 cut(s) 518, 1085
Eco47III AGCGCT 2 cut(s) 45, 525
Eco57I CTGAAG 1 cut(s) 432
Eco88I CYCGRG 2 cut(s) 107, 483
EcoT14I CCWWGG 2 cut(s) 52, 376
EcoT22I ATGCAT 1 cut(s) 239
ErhI CCWWGG 2 cut(s) 52, 376
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 7 cut(s) 56, 466, 617, 703, 857, 896, 1114
FalI AAGNNNNNCTT 4 cut(s) 765, 797, 1170, 1202
FatI CATG 7 cut(s) 52, 462, 613, 699, 853, 892, 1110
FauI CCCGC 1 cut(s) 627
FbaI TGATCA 2 cut(s) 225, 630
FblI GTMKAC 1 cut(s) 27
FokI GGATG 3 cut(s) 581, 754, 831
FspBI CTAG 2 cut(s) 1026, 1217
GlaI GCGC 3 cut(s) 45, 342, 525
HaeII RGCGCY 2 cut(s) 47, 527
HaeIII GGCC 7 cut(s) 105, 141, 168, 250, 427, 593, 1038
HapII CCGG 2 cut(s) 221, 344
HgaI GACGC 1 cut(s) 122
HhaI GCGC 3 cut(s) 46, 343, 526
Hin1I GRCGYC 1 cut(s) 281
Hin1II CATG 7 cut(s) 56, 466, 617, 703, 857, 896, 1114
Hin6I GCGC 3 cut(s) 44, 341, 524
HinP1I GCGC 3 cut(s) 44, 341, 524
HincII GTYRAC 1 cut(s) 825
HindII GTYRAC 1 cut(s) 825
HinfI GANTC 4 cut(s) 217, 368, 1288, 1306
HpaI GTTAAC 1 cut(s) 825
HpaII CCGG 2 cut(s) 221, 344
HphI GGTGA 7 cut(s) 54, 71, 212, 235, 656, 977, 1214
Hpy166II GTNNAC 7 cut(s) 28, 37, 367, 419, 718, 825, 955
Hpy188I TCNGA 7 cut(s) 101, 122, 267, 451, 682, 1051, 1305
Hpy188III TCNNGA 2 cut(s) 516, 1217
Hpy8I GTNNAC 7 cut(s) 28, 37, 367, 419, 718, 825, 955
Hpy99I CGWCG 1 cut(s) 283
HpyAV CCTTC 8 cut(s) 86, 156, 158, 197, 366, 727, 881, 1027
HpyCH4III ACNGT 2 cut(s) 722, 1131
HpyCH4IV ACGT 5 cut(s) 281, 310, 508, 916, 1281
HpyCH4V TGCA 8 cut(s) 237, 589, 613, 1061, 1073, 1126, 1153, 1235
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 619, 1035, 1061, 1070, 1229
HpyF3I CTNAG 2 cut(s) 681, 1187
HpySE526I ACGT 5 cut(s) 281, 310, 508, 916, 1281
Hsp92I GRCGYC 1 cut(s) 281
Hsp92II CATG 7 cut(s) 56, 466, 617, 703, 857, 896, 1114
HspAI GCGC 3 cut(s) 44, 341, 524
Ksp22I TGATCA 2 cut(s) 225, 630
KspAI GTTAAC 1 cut(s) 825
Kzo9I GATC 4 cut(s) 225, 567, 630, 690
LguI GCTCTTC 1 cut(s) 8
LweI GCATC 1 cut(s) 537
MaeI CTAG 2 cut(s) 1026, 1217
MaeII ACGT 5 cut(s) 281, 310, 508, 916, 1281
MaeIII GTNAC 2 cut(s) 380, 644
MalI GATC 4 cut(s) 227, 569, 632, 692
MboI GATC 4 cut(s) 225, 567, 630, 690
MboII GAAGA 8 cut(s) 25, 305, 521, 716, 741, 779, 782, 1190
MhlI GDGCHC 2 cut(s) 114, 727
MlsI TGGCCA 2 cut(s) 427, 1038
MluCI AATT 7 cut(s) 430, 435, 668, 875, 968, 1005, 1166
MluNI TGGCCA 2 cut(s) 427, 1038
MlyI GAGTC 1 cut(s) 362
MmeI TCCRAC 2 cut(s) 341, 643
Mox20I TGGCCA 2 cut(s) 427, 1038
Mph1103I ATGCAT 1 cut(s) 239
MscI TGGCCA 2 cut(s) 427, 1038
MseI TTAA 7 cut(s) 257, 434, 476, 504, 710, 824, 1133
MslI CAYNNNNRTG 1 cut(s) 121
Msp20I TGGCCA 2 cut(s) 427, 1038
MspA1I CMGCKG 2 cut(s) 244, 607
MspI CCGG 2 cut(s) 221, 344
Mva1269I GAATGC 2 cut(s) 237, 786
MwoI GCNNNNNNNGC 6 cut(s) 165, 619, 1035, 1061, 1070, 1229
NcoI CCATGG 1 cut(s) 52
NdeII GATC 4 cut(s) 225, 567, 630, 690
NlaIII CATG 7 cut(s) 56, 466, 617, 703, 857, 896, 1114
NlaIV GGNNCC 2 cut(s) 182, 1015
NmuCI GTSAC 1 cut(s) 644
NsiI ATGCAT 1 cut(s) 239
NspI RCATGY 1 cut(s) 617
PaeI GCATGC 1 cut(s) 617
PaeR7I CTCGAG 2 cut(s) 107, 483
PceI AGGCCT 2 cut(s) 105, 250
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PctI GAATGC 2 cut(s) 237, 786
PfeI GAWTC 3 cut(s) 217, 1288, 1306
PflMI CCANNNNNTGG 1 cut(s) 854
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
PsiI TTATAA 1 cut(s) 984
Psp1406I AACGTT 2 cut(s) 508, 1281
PspN4I GGNNCC 2 cut(s) 182, 1015
PspPI GGNCC 4 cut(s) 139, 167, 518, 1085
PspXI VCTCGAGB 2 cut(s) 107, 483
PstI CTGCAG 3 cut(s) 1063, 1155, 1237
PstNI CAGNNNCTG 2 cut(s) 610, 1055
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 4 cut(s) 74, 717, 812, 1116
RsaNI GTAC 4 cut(s) 73, 716, 811, 1115
RseI CAYNNNNRTG 1 cut(s) 121
SapI GCTCTTC 1 cut(s) 8
SaqAI TTAA 7 cut(s) 257, 434, 476, 504, 710, 824, 1133
Sau3AI GATC 4 cut(s) 225, 567, 630, 690
Sau96I GGNCC 4 cut(s) 139, 167, 518, 1085
SchI GAGTC 1 cut(s) 362
SduI GDGCHC 2 cut(s) 114, 727
SfaNI GCATC 1 cut(s) 537
SfcI CTRYAG 4 cut(s) 996, 1059, 1151, 1233
Sfr274I CTCGAG 2 cut(s) 107, 483
SinI GGWCC 2 cut(s) 518, 1085
SlaI CTCGAG 2 cut(s) 107, 483
SmiMI CAYNNNNRTG 1 cut(s) 121
SmlI CTYRAG 2 cut(s) 107, 483
SmoI CTYRAG 2 cut(s) 107, 483
SphI GCATGC 1 cut(s) 617
Sse9I AATT 7 cut(s) 430, 435, 668, 875, 968, 1005, 1166
SseBI AGGCCT 2 cut(s) 105, 250
SsiI CCGC 4 cut(s) 5, 91, 244, 620
SspMI CTAG 2 cut(s) 1026, 1217
StuI AGGCCT 2 cut(s) 105, 250
StyI CCWWGG 2 cut(s) 52, 376
TaaI ACNGT 2 cut(s) 722, 1131
TaiI ACGT 5 cut(s) 284, 313, 511, 919, 1284
TaqI TCGA 4 cut(s) 108, 348, 484, 841
TasI AATT 7 cut(s) 430, 435, 668, 875, 968, 1005, 1166
TatI WGTACW 1 cut(s) 715
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 217, 1288, 1306
Tru1I TTAA 7 cut(s) 257, 434, 476, 504, 710, 824, 1133
Tru9I TTAA 7 cut(s) 257, 434, 476, 504, 710, 824, 1133
TscAI CASTG 2 cut(s) 92, 725
TseFI GTSAC 1 cut(s) 644
Tsp45I GTSAC 1 cut(s) 644
TspDTI ATGAA 2 cut(s) 531, 552
TspRI CASTG 2 cut(s) 92, 725
Van91I CCANNNNNTGG 1 cut(s) 854
VpaK11BI GGWCC 2 cut(s) 518, 1085
XapI RAATTY 2 cut(s) 430, 668
XbaI TCTAGA 1 cut(s) 1216
XceI RCATGY 1 cut(s) 617
XhoI CTCGAG 2 cut(s) 107, 483
XmiI GTMKAC 1 cut(s) 27
XspI CTAG 2 cut(s) 1026, 1217
ZraI GACGTC 1 cut(s) 282
Zsp2I ATGCAT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.