pycom14g09540

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009299
Physical Location & Seq
Forward (+)
11517676 .. 11518681
1006 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 465 bp
ATGGAATCATTGCGTCTCTTCCCACATCCTCCTGTTTTGATAAGAAGAGCTCAAGTAGCTGACGTGCTCCCGGGAAATTACAAAGTCAATGCTGGTCAAGATATTATGATATCAGTATACAATATCCATCGTTCTTCAAAGGTGTGGGAGAGAGCAGAAGACTATGTACCTGAAAGATTTGATCTTGAAGGCTCCGTACCTAATGAAACAAACACAGATTTCAGGTTCATTCCATTCAGCGGAGGGCCACGGAAGTGTGTTGGTGATCAATTTGCTCTGCTGGAAGCGACTGTTGCTCTCACAATCTTTATTCAGAACCTGAACTTTGAGCTGGTTCCGGATCAGAAAATTAGCATGACGACCGGAGCAACAATTCACACCACAAATGGTTTGTACATGAAGTTAAGCCAACGACAGGCAAAATCTGCATTCGCTACATCATCCTCGTCTTCGTCCGCCTCTTAG

Protein Analysis

155

Amino Acids

17.1

Weight (kDa)

6.83

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 2 - 122 4.8e-31 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 117
AccIII TCCGGA 1 cut(s) 337
AciI CCGC 2 cut(s) 240, 456
AclWI GGATC 1 cut(s) 348
AfaI GTAC 3 cut(s) 168, 198, 395
AfiI CCNNNNNNNGG 2 cut(s) 239, 415
AgsI TTSAA 2 cut(s) 138, 188
AjiI CACGTC 1 cut(s) 64
AleI CACNNNNGTG 1 cut(s) 253
AluBI AGCT 3 cut(s) 50, 59, 331
AluI AGCT 3 cut(s) 50, 59, 331
Alw21I GWGCWC 2 cut(s) 52, 69
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 1 cut(s) 348
AlwNI CAGNNNCTG 1 cut(s) 319
Ama87I CYCGRG 1 cut(s) 70
Aor13HI TCCGGA 1 cut(s) 337
AoxI GGCC 1 cut(s) 245
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 2 cut(s) 71, 72
AsuHPI GGTGA 1 cut(s) 275
AvaI CYCGRG 1 cut(s) 70
BanII GRGCYC 1 cut(s) 52
BarI GAAGNNNNNNTAC 4 cut(s) 150, 180, 182, 212
BbsI GAAGAC 2 cut(s) 165, 441
Bbv12I GWGCWC 2 cut(s) 52, 69
BccI CCATC 1 cut(s) 135
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 2 cut(s) 71, 72
BcoDI GTCTC 1 cut(s) 20
Bme1390I CCNGG 2 cut(s) 71, 72
BmeT110I CYCGRG 1 cut(s) 70
BmgBI CACGTC 1 cut(s) 64
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 193, 336
BmrFI CCNGG 2 cut(s) 71, 72
BpiI GAAGAC 2 cut(s) 165, 441
BpuEI CTTGAG 1 cut(s) 36
BpuMI CCSGG 2 cut(s) 71, 72
BsaJI CCNNGG 2 cut(s) 70, 248
BsaWI WCCGGW 2 cut(s) 337, 362
Bsc4I CCNNNNNNNGG 2 cut(s) 239, 415
Bse3DI GCAATG 1 cut(s) 8
BseAI TCCGGA 1 cut(s) 337
BseDI CCNNGG 2 cut(s) 70, 248
BseGI GGATG 2 cut(s) 25, 440
BseLI CCNNNNNNNGG 2 cut(s) 239, 415
BseMI GCAATG 1 cut(s) 8
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 1 cut(s) 247
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 2 cut(s) 52, 69
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 3 cut(s) 71, 338, 363
BslI CCNNNNNNNGG 2 cut(s) 239, 415
BsmAI GTCTC 1 cut(s) 20
BsmBI CGTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 428
BsnI GGCC 1 cut(s) 247
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 52, 69
Bsp13I TCCGGA 1 cut(s) 337
Bsp1407I TGTACA 1 cut(s) 393
Bsp143I GATC 3 cut(s) 181, 265, 340
BspACI CCGC 2 cut(s) 240, 456
BspANI GGCC 1 cut(s) 247
BspEI TCCGGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 193, 336
BspPI GGATC 1 cut(s) 348
BspQI GCTCTTC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 8
BsrGI TGTACA 1 cut(s) 393
BssECI CCNNGG 2 cut(s) 70, 248
BssMI GATC 3 cut(s) 181, 265, 340
BssNAI GTATAC 1 cut(s) 118
Bst1107I GTATAC 1 cut(s) 118
Bst4CI ACNGT 1 cut(s) 292
Bst6I CTCTTC 2 cut(s) 23, 40
BstAPI GCANNNNNTGC 1 cut(s) 425
BstAUI TGTACA 1 cut(s) 393
BstDEI CTNAG 1 cut(s) 462
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 2 cut(s) 25, 440
BstKTI GATC 3 cut(s) 184, 268, 343
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 3 cut(s) 181, 265, 340
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 3 cut(s) 56, 293, 425
BstSCI CCNGG 2 cut(s) 69, 70
BstV2I GAAGAC 2 cut(s) 165, 441
BstZ17I GTATAC 1 cut(s) 118
BsuRI GGCC 1 cut(s) 247
BtgI CCRYGG 1 cut(s) 248
BtrI CACGTC 1 cut(s) 64
BtsCI GGATG 2 cut(s) 25, 440
CaiI CAGNNNCTG 1 cut(s) 319
Cfr13I GGNCC 1 cut(s) 245
Cfr9I CCCGGG 1 cut(s) 70
CseI GACGC 1 cut(s) 2
Csp6I GTAC 3 cut(s) 167, 197, 394
CviAII CATG 2 cut(s) 355, 397
CviJI RGCY 6 cut(s) 50, 59, 192, 247, 331, 408
CviKI_1 RGCY 6 cut(s) 50, 59, 192, 247, 331, 408
CviQI GTAC 3 cut(s) 167, 197, 394
DdeI CTNAG 1 cut(s) 462
DpnI GATC 3 cut(s) 183, 267, 342
DpnII GATC 3 cut(s) 181, 265, 340
Eam1104I CTCTTC 2 cut(s) 23, 40
EarI CTCTTC 2 cut(s) 23, 40
EciI GGCGGA 1 cut(s) 445
Ecl136II GAGCTC 1 cut(s) 50
Eco24I GRGCYC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 111
Eco53kI GAGCTC 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 50
EcoRV GATATC 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 52
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 2 cut(s) 358, 400
FaiI YATR 5 cut(s) 107, 118, 165, 356, 398
FatI CATG 2 cut(s) 354, 396
FbaI TGATCA 1 cut(s) 265
FblI GTMKAC 1 cut(s) 117
FokI GGATG 2 cut(s) 12, 427
FriOI GRGCYC 1 cut(s) 52
HaeIII GGCC 1 cut(s) 247
HapII CCGG 3 cut(s) 71, 338, 363
HgaI GACGC 1 cut(s) 2
Hin1II CATG 2 cut(s) 358, 400
HinfI GANTC 1 cut(s) 5
HpaII CCGG 3 cut(s) 71, 338, 363
HphI GGTGA 1 cut(s) 275
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 2 cut(s) 315, 345
Hpy188III TCNNGA 3 cut(s) 98, 185, 338
Hpy8I GTNNAC 1 cut(s) 118
HpyAV CCTTC 1 cut(s) 182
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 1 cut(s) 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 293, 425
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 2 cut(s) 358, 400
Kpn2I TCCGGA 1 cut(s) 337
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 3 cut(s) 181, 265, 340
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 3 cut(s) 72, 197, 365
MaeII ACGT 1 cut(s) 63
MalI GATC 3 cut(s) 183, 267, 342
MboI GATC 3 cut(s) 181, 265, 340
MboII GAAGA 5 cut(s) 10, 57, 126, 170, 441
MhlI GDGCHC 2 cut(s) 52, 69
MluCI AATT 4 cut(s) 76, 269, 348, 372
MnlI CCTC 3 cut(s) 39, 236, 454
MroI TCCGGA 1 cut(s) 337
MseI TTAA 1 cut(s) 404
MslI CAYNNNNRTG 1 cut(s) 253
MspA1I CMGCKG 1 cut(s) 240
MspI CCGG 3 cut(s) 71, 338, 363
MspR9I CCNGG 2 cut(s) 71, 72
Mva1269I GAATGC 1 cut(s) 428
MwoI GCNNNNNNNGC 3 cut(s) 56, 293, 425
NciI CCSGG 2 cut(s) 71, 72
NdeII GATC 3 cut(s) 181, 265, 340
NlaIII CATG 2 cut(s) 358, 400
NlaIV GGNNCC 2 cut(s) 193, 336
OliI CACNNNNGTG 1 cut(s) 253
PciSI GCTCTTC 1 cut(s) 40
PctI GAATGC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 5
Psp124BI GAGCTC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 193, 336
PspPI GGNCC 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 319
RsaI GTAC 3 cut(s) 168, 198, 395
RsaNI GTAC 3 cut(s) 167, 197, 394
RseI CAYNNNNRTG 1 cut(s) 253
SacI GAGCTC 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 40
SaqAI TTAA 1 cut(s) 404
Sau3AI GATC 3 cut(s) 181, 265, 340
Sau96I GGNCC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 71, 72
SduI GDGCHC 2 cut(s) 52, 69
SetI ASST 9 cut(s) 52, 61, 66, 144, 172, 202, 227, 321, 333
SmaI CCCGGG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 51
SmoI CTYRAG 1 cut(s) 51
Sse9I AATT 4 cut(s) 76, 269, 348, 372
SsiI CCGC 2 cut(s) 240, 456
SstI GAGCTC 1 cut(s) 52
StyD4I CCNGG 2 cut(s) 69, 70
TaaI ACNGT 1 cut(s) 292
TaiI ACGT 1 cut(s) 66
TasI AATT 4 cut(s) 76, 269, 348, 372
TatI WGTACW 1 cut(s) 393
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 404
Tru9I TTAA 1 cut(s) 404
TspDTI ATGAA 3 cut(s) 217, 219, 413
TspGWI ACGGA 2 cut(s) 184, 265
TspMI CCCGGG 1 cut(s) 70
XmaI CCCGGG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.