pycom14g10650

Det1 complexing ubiquitin ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013398
Physical Location & Seq
Reverse (-)
13385261 .. 13387849
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 294 bp
ATGGGGTCTTTGTTCGGTGACTGGCCGTCGTATAACCCTCACAACTTCAGCCAGGTCCGACCGTCTGATCATTCAAATCCTTCTAAAATGACACCTGCAACCTACCATCCTACCCACGACCGGACTCTTCCGCCACCTGATCAAGTGATAACTAATGAAGCCAAGAACATCCTTTTGAGGCACATGTATCAGCATTCTGAAGAGAAGTTGAGACAAAAGCGGGCAGCGCCGGAAAAACTTTCACCAGAGCCTGGATGCAAGCAACTTAGGTCTTCCGTTTCAGATAATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDA1

Protein Analysis

98

Amino Acids

11.03

Weight (kDa)

8.85

Isoelectric Point (pI)

74.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDA1 PF10172 3 - 68 2.7e-29 Det1 complexing ubiquitin ligase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G41560 AT5G41560
fragaria_vesca FvH4_5g03180 FvH4_5g03180 FvH4_5g03180 FvH4_5g03180
malus_domestica MD06G1090900.v1.1 MD14G1111700.v1.1
prunus_persica Prupe.5G106400_v2.0.a1
pyrus_communis pycom06g08650 pycom14g10650
rosa_chinensis RchiOBHm_Chr7g0196601
rosa_multiflora Rmu_sc0006080.1_g000005
rosa_roxburghii Rroxscaffold_3G00259170
rosa_rugosa Rorug07G0035200 Rorug07G0035300
rosa_samantha Rh7DG160300
rosa_wichuraiana Rw7G013910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 103
Acc36I ACCTGC 1 cut(s) 103
AccB7I CCANNNNNTGG 1 cut(s) 251
AciI CCGC 2 cut(s) 131, 220
AcoI YGGCCR 1 cut(s) 23
AcuI CTGAAG 2 cut(s) 31, 219
AfiI CCNNNNNNNGG 2 cut(s) 120, 251
AflIII ACRYGT 1 cut(s) 183
AgsI TTSAA 1 cut(s) 75
AhdI GACNNNNNGTC 1 cut(s) 25
AjnI CCWGG 2 cut(s) 51, 250
Alw26I GTCTC 1 cut(s) 205
AlwNI CAGNNNCTG 1 cut(s) 251
AoxI GGCC 1 cut(s) 23
ApeKI GCWGC 1 cut(s) 224
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 55
AsuHPI GGTGA 2 cut(s) 29, 234
AvaII GGWCC 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 264
BbvI GCAGC 1 cut(s) 236
BccI CCATC 1 cut(s) 114
BceAI ACGGC 1 cut(s) 10
BciT130I CCWGG 2 cut(s) 53, 252
BclI TGATCA 2 cut(s) 67, 139
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 292
BfoI RGCGCY 1 cut(s) 230
BfuAI ACCTGC 1 cut(s) 103
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
Bme1390I CCNGG 2 cut(s) 53, 252
Bme18I GGWCC 1 cut(s) 55
BmeRI GACNNNNNGTC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 55
BmrFI CCNGG 2 cut(s) 53, 252
BmsI GCATC 1 cut(s) 245
BpiI GAAGAC 1 cut(s) 264
BsaWI WCCGGW 1 cut(s) 120
Bsc4I CCNNNNNNNGG 2 cut(s) 120, 251
Bse1I ACTGG 1 cut(s) 26
BseBI CCWGG 2 cut(s) 53, 252
BseGI GGATG 3 cut(s) 106, 168, 260
BseLI CCNNNNNNNGG 2 cut(s) 120, 251
BseNI ACTGG 1 cut(s) 26
BseXI GCAGC 1 cut(s) 236
Bsh1285I CGRYCG 2 cut(s) 62, 121
BshFI GGCC 1 cut(s) 25
BsiEI CGRYCG 2 cut(s) 62, 121
BsiSI CCGG 2 cut(s) 121, 230
BslI CCNNNNNNNGG 2 cut(s) 120, 251
BsmAI GTCTC 1 cut(s) 205
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 1 cut(s) 25
Bsp143I GATC 2 cut(s) 67, 139
BspACI CCGC 2 cut(s) 131, 220
BspANI GGCC 1 cut(s) 25
BspMI ACCTGC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 26
BssMI GATC 2 cut(s) 67, 139
Bst2UI CCWGG 2 cut(s) 53, 252
Bst4CI ACNGT 1 cut(s) 63
Bst6I CTCTTC 2 cut(s) 132, 195
BstC8I GCNNGC 2 cut(s) 222, 260
BstDEI CTNAG 1 cut(s) 266
BstF5I GGATG 3 cut(s) 106, 168, 260
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 2 cut(s) 70, 142
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 67, 139
BstMCI CGRYCG 2 cut(s) 62, 121
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNI CCWGG 2 cut(s) 53, 252
BstNSI RCATGY 1 cut(s) 187
BstSCI CCNGG 2 cut(s) 51, 250
BstV1I GCAGC 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 264
BsuRI GGCC 1 cut(s) 25
BtsCI GGATG 3 cut(s) 106, 168, 260
BveI ACCTGC 1 cut(s) 103
Cac8I GCNNGC 2 cut(s) 222, 260
CaiI CAGNNNCTG 1 cut(s) 251
CfoI GCGC 1 cut(s) 229
Cfr13I GGNCC 1 cut(s) 55
CviAII CATG 1 cut(s) 184
CviJI RGCY 4 cut(s) 25, 51, 161, 250
CviKI_1 RGCY 4 cut(s) 25, 51, 161, 250
DdeI CTNAG 1 cut(s) 266
DpnI GATC 2 cut(s) 69, 141
DpnII GATC 2 cut(s) 67, 139
DriI GACNNNNNGTC 1 cut(s) 25
EaeI YGGCCR 1 cut(s) 23
Eam1104I CTCTTC 2 cut(s) 132, 195
Eam1105I GACNNNNNGTC 1 cut(s) 25
EarI CTCTTC 2 cut(s) 132, 195
EciI GGCGGA 1 cut(s) 120
Eco47I GGWCC 1 cut(s) 55
Eco57I CTGAAG 2 cut(s) 31, 219
EcoRII CCWGG 2 cut(s) 51, 250
FaeI CATG 1 cut(s) 187
FaiI YATR 2 cut(s) 33, 185
FatI CATG 1 cut(s) 183
FauI CCCGC 1 cut(s) 213
FbaI TGATCA 2 cut(s) 67, 139
Fnu4HI GCNGC 1 cut(s) 225
FokI GGATG 3 cut(s) 93, 155, 267
Fsp4HI GCNGC 1 cut(s) 225
FspBI CTAG 1 cut(s) 292
GlaI GCGC 1 cut(s) 228
GluI GCNGC 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 1 cut(s) 25
HapII CCGG 2 cut(s) 121, 230
HhaI GCGC 1 cut(s) 229
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HinfI GANTC 1 cut(s) 124
HpaII CCGG 2 cut(s) 121, 230
HphI GGTGA 2 cut(s) 29, 234
Hpy188I TCNGA 4 cut(s) 59, 67, 199, 283
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 1 cut(s) 90
HpyCH4III ACNGT 1 cut(s) 63
HpyCH4V TGCA 2 cut(s) 98, 258
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 1 cut(s) 266
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 227
Ksp22I TGATCA 2 cut(s) 67, 139
Kzo9I GATC 2 cut(s) 67, 139
Lsp1109I GCAGC 1 cut(s) 236
LweI GCATC 1 cut(s) 245
MaeI CTAG 1 cut(s) 292
MaeIII GTNAC 1 cut(s) 17
MalI GATC 2 cut(s) 69, 141
MboI GATC 2 cut(s) 67, 139
MboII GAAGA 3 cut(s) 119, 212, 264
MlyI GAGTC 1 cut(s) 118
MmeI TCCRAC 1 cut(s) 82
MnlI CCTC 2 cut(s) 48, 171
MspI CCGG 2 cut(s) 121, 230
MspR9I CCNGG 2 cut(s) 53, 252
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 2 cut(s) 53, 252
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeII GATC 2 cut(s) 67, 139
NlaIII CATG 1 cut(s) 187
NmuCI GTSAC 1 cut(s) 17
NspI RCATGY 1 cut(s) 187
PaqCI CACCTGC 1 cut(s) 103
PciI ACATGT 1 cut(s) 183
PctI GAATGC 1 cut(s) 193
PflMI CCANNNNNTGG 1 cut(s) 251
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 118
PpsI GAGTC 1 cut(s) 118
PscI ACATGT 1 cut(s) 183
Psp6I CCWGG 2 cut(s) 51, 250
PspGI CCWGG 2 cut(s) 51, 250
PspPI GGNCC 1 cut(s) 55
PstNI CAGNNNCTG 1 cut(s) 251
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 2 cut(s) 67, 139
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 2 cut(s) 53, 252
SetI ASST 5 cut(s) 57, 97, 104, 139, 272
SfaNI GCATC 1 cut(s) 245
SinI GGWCC 1 cut(s) 55
SsiI CCGC 2 cut(s) 131, 220
SspMI CTAG 1 cut(s) 292
StyD4I CCNGG 2 cut(s) 51, 250
TaaI ACNGT 1 cut(s) 63
TseFI GTSAC 1 cut(s) 17
TseI GCWGC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 17
TspDTI ATGAA 1 cut(s) 171
TspGWI ACGGA 1 cut(s) 265
Van91I CCANNNNNTGG 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 55
XceI RCATGY 1 cut(s) 187
XspI CTAG 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.