pycom14g15290

Ribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0015311
Physical Location & Seq
Forward (+)
17947626 .. 17948850
1225 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 549 bp
ATGCCTCGTCATGGTTTCGCCCTCCTCGCCGTTTTCGTCCTCGCAATCCTTCCTCATATTCAGGGTTCGGTGAACGATGATCAGATCTCCGTACGATCATCGCCGTTCGAAACAGCCCTCGAAACCCTTCAGGTGCAAATTGGGTATAAGTTCAAAAAAATTAGTCTCCTGCGCCGTGCCATGACCCATGCCTCCTTCTCCGAAGAGAACAACAAGGCACTGAGCATTTTGGGTGCCAATGTCATAGAAACATCGGTCTCTATGAGATTACTTGAAAACAACCTTGAGATATCTGCAAAAGAGTTGAACCGCCGCATTTCTGAGGTCTCAAAAGTAGAATCTTCCTGTGCTACTGACGGGAAGCAATTGGGGTTGCATAAGGTGGTTAGAGTCTCTCCTAAGACCGATTCTTCAACTCCCTTGGTGGTTTGTGGGGCTTTCAGAGCGGTCTTTGGTGCAATTGCTCTTGATACTGAGAAGGCTGATGATGCTGGAAGTTATTTTTGGCGTGTTCATGGTGGCCAAGTTGGAGGAGCTCTTGCAATGTAA

Protein Analysis

183

Amino Acids

19.7

Weight (kDa)

8.6

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonucleas_3_3 PF14622 55 - 158 2.3e-12 Ribonuclease-III-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015311)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G24450
fragaria_vesca FvH4_5g05090
malus_domestica MD14G1183800.v1.1
prunus_persica Prupe.5G176500_v2.0.a1
pyrus_communis pycom14g15290
rosa_chinensis RchiOBHm_Chr7g0193911
rosa_laevigata RLG00000004234 RLG00000028758
rosa_multiflora Rmu_sc0002389.1_g000010
rosa_roxburghii Rroxscaffold_3G00261710
rosa_rugosa Rorug07G0012800
rosa_samantha Rh7AG139600 Rh7BG140100 Rh7CG143800 Rh7DG141000
rosa_wichuraiana Rw7G011910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccBSI CCGCTC 1 cut(s) 446
AciI CCGC 3 cut(s) 310, 313, 446
AcoI YGGCCR 1 cut(s) 520
AcuI CTGAAG 1 cut(s) 113
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 1 cut(s) 11
AgsI TTSAA 4 cut(s) 154, 275, 307, 414
AjuI GAANNNNNNNTTGG 2 cut(s) 487, 519
AluBI AGCT 1 cut(s) 536
AluI AGCT 1 cut(s) 536
Alw21I GWGCWC 1 cut(s) 538
Alw26I GTCTC 4 cut(s) 170, 262, 331, 397
AoxI GGCC 1 cut(s) 520
Asp700I GAANNNNTTC 1 cut(s) 126
AspLEI GCGC 1 cut(s) 174
AsuHPI GGTGA 1 cut(s) 82
AsuII TTCGAA 1 cut(s) 108
BalI TGGCCA 1 cut(s) 522
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 1 cut(s) 538
Bbv12I GWGCWC 1 cut(s) 538
BceAI ACGGC 3 cut(s) 14, 88, 159
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 4 cut(s) 170, 262, 331, 397
BglII AGATCT 1 cut(s) 84
BisI GCNGC 1 cut(s) 313
BlsI GCNGC 1 cut(s) 314
BmiI GGNNCC 1 cut(s) 235
BmsI GCATC 1 cut(s) 478
Bpu14I TTCGAA 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 305
BsaI GGTCTC 2 cut(s) 262, 331
BsaJI CCNNGG 1 cut(s) 420
Bsc4I CCNNNNNNNGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 420
BseLI CCNNNNNNNGG 1 cut(s) 11
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 3 cut(s) 212, 312, 465
BseRI GAGGAG 2 cut(s) 14, 546
BshFI GGCC 1 cut(s) 522
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 538
BsiWI CGTACG 1 cut(s) 91
BslI CCNNNNNNNGG 1 cut(s) 11
BsmAI GTCTC 4 cut(s) 170, 262, 331, 397
BsnI GGCC 1 cut(s) 522
Bso31I GGTCTC 2 cut(s) 262, 331
Bsp119I TTCGAA 1 cut(s) 108
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 3 cut(s) 79, 84, 95
BspACI CCGC 3 cut(s) 310, 313, 446
BspANI GGCC 1 cut(s) 522
BspCNI CTCAG 3 cut(s) 213, 313, 466
BspLI GGNNCC 1 cut(s) 235
BspT104I TTCGAA 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 2 cut(s) 262, 331
BsrBI CCGCTC 1 cut(s) 446
BsrDI GCAATG 1 cut(s) 549
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 3 cut(s) 79, 84, 95
BssT1I CCWWGG 1 cut(s) 420
Bst6I CTCTTC 1 cut(s) 198
BstBI TTCGAA 1 cut(s) 108
BstDEI CTNAG 4 cut(s) 221, 321, 399, 474
BstHHI GCGC 1 cut(s) 174
BstKTI GATC 3 cut(s) 82, 87, 98
BstMAI GTCTC 4 cut(s) 170, 262, 331, 397
BstMBI GATC 3 cut(s) 79, 84, 95
BstMWI GCNNNNNNNGC 3 cut(s) 26, 443, 488
BstX2I RGATCY 1 cut(s) 84
BstYI RGATCY 1 cut(s) 84
BsuRI GGCC 1 cut(s) 522
BtgZI GCGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 218
CfoI GCGC 1 cut(s) 174
Csp6I GTAC 1 cut(s) 92
CviAII CATG 4 cut(s) 11, 181, 188, 515
CviJI RGCY 5 cut(s) 116, 437, 482, 522, 536
CviKI_1 RGCY 5 cut(s) 116, 437, 482, 522, 536
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 4 cut(s) 221, 321, 399, 474
DpnI GATC 3 cut(s) 81, 86, 97
DpnII GATC 3 cut(s) 79, 84, 95
EaeI YGGCCR 1 cut(s) 520
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Ecl136II GAGCTC 1 cut(s) 536
Eco130I CCWWGG 1 cut(s) 420
Eco24I GRGCYC 1 cut(s) 538
Eco31I GGTCTC 2 cut(s) 262, 331
Eco32I GATATC 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 536
Eco57I CTGAAG 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 536
EcoRV GATATC 1 cut(s) 291
EcoT14I CCWWGG 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 4 cut(s) 14, 184, 191, 518
FaiI YATR 9 cut(s) 12, 57, 147, 182, 189, 245, 263, 378, 516
FatI CATG 4 cut(s) 10, 180, 187, 514
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 1 cut(s) 313
FriOI GRGCYC 1 cut(s) 538
Fsp4HI GCNGC 1 cut(s) 313
GlaI GCGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 313
HaeIII GGCC 1 cut(s) 522
HhaI GCGC 1 cut(s) 174
Hin1II CATG 4 cut(s) 14, 184, 191, 518
Hin6I GCGC 1 cut(s) 172
HinP1I GCGC 1 cut(s) 172
HinfI GANTC 3 cut(s) 338, 390, 407
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 73
Hpy188I TCNGA 4 cut(s) 84, 202, 322, 443
Hpy188III TCNNGA 1 cut(s) 467
Hpy8I GTNNAC 1 cut(s) 73
HpyAV CCTTC 4 cut(s) 59, 137, 205, 472
HpyCH4V TGCA 5 cut(s) 136, 296, 376, 458, 542
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 443, 488
HpyF3I CTNAG 4 cut(s) 221, 321, 399, 474
Hsp92II CATG 4 cut(s) 14, 184, 191, 518
HspAI GCGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 3 cut(s) 79, 84, 95
LmnI GCTCC 1 cut(s) 533
LpnPI CCDG 5 cut(s) 47, 116, 182, 358, 477
LweI GCATC 1 cut(s) 478
MalI GATC 3 cut(s) 81, 86, 97
MbiI CCGCTC 1 cut(s) 446
MboI GATC 3 cut(s) 79, 84, 95
MboII GAAGA 3 cut(s) 215, 333, 402
MfeI CAATTG 2 cut(s) 365, 459
MflI RGATCY 1 cut(s) 84
MhlI GDGCHC 1 cut(s) 538
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 4 cut(s) 138, 159, 365, 459
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 1 cut(s) 399
MmeI TCCRAC 1 cut(s) 508
MnlI CCTC 9 cut(s) 15, 32, 35, 50, 63, 128, 202, 316, 524
Mox20I TGGCCA 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 126
MscI TGGCCA 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 522
MunI CAATTG 2 cut(s) 365, 459
MwoI GCNNNNNNNGC 3 cut(s) 26, 443, 488
NdeII GATC 3 cut(s) 79, 84, 95
NlaIII CATG 4 cut(s) 14, 184, 191, 518
NlaIV GGNNCC 1 cut(s) 235
NspV TTCGAA 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 2 cut(s) 338, 407
Pfl23II CGTACG 1 cut(s) 91
PkrI GCNGC 1 cut(s) 314
PleI GAGTC 1 cut(s) 398
PpsI GAGTC 1 cut(s) 398
Psp124BI GAGCTC 1 cut(s) 538
PspLI CGTACG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 235
PsuI RGATCY 1 cut(s) 84
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
SacI GAGCTC 1 cut(s) 538
SatI GCNGC 1 cut(s) 313
Sau3AI GATC 3 cut(s) 79, 84, 95
SchI GAGTC 1 cut(s) 399
SduI GDGCHC 1 cut(s) 538
SetI ASST 5 cut(s) 135, 285, 327, 384, 538
SfaNI GCATC 1 cut(s) 478
SfuI TTCGAA 1 cut(s) 108
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 4 cut(s) 138, 159, 365, 459
SsiI CCGC 3 cut(s) 310, 313, 446
SstI GAGCTC 1 cut(s) 538
StyI CCWWGG 1 cut(s) 420
TaqI TCGA 2 cut(s) 108, 120
TaqII GACCGA 2 cut(s) 244, 419
TasI AATT 4 cut(s) 138, 159, 365, 459
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 2 cut(s) 338, 407
TscAI CASTG 1 cut(s) 225
TspDTI ATGAA 1 cut(s) 503
TspGWI ACGGA 1 cut(s) 79
TspRI CASTG 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.