pycom14g16430

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013366
Physical Location & Seq
Forward (+)
18709271 .. 18712453
3183 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGGCTTCCAACATTGGGATAATGGACAGTGCTTACTTTGTTGGGAGAAATGAGATTTTGACGTGGATCAACAATCGCCTTCAGCTGAATCTCTCTCGCATTGAAGAGGCTGCATCTGGTGCTGTACAATGTCAAATGATGGATATGACCTATCCTGGTGTTGTTCCAATGCACAAGGTCAATTTTGATGCGAAAACAGAATATGATATGATCCAGAACTATAAGGTTCTACAGGAAGTATTCAACAAATTAAAAATTGCGAAGCATCTTGAAGTCAATAGGCTTGTTAAAGGCAGGCCTTTGGACAACTTGGAATTCTTGCAATGGCTGAAACGTTACTGTGATTCCATAAATGGTGGCATTATGAATGAAAACTATAACCCTGTGGAGCGAAGGAGTAAGGGTGGGAAGGATCGACATCCCAAAGGTGCTCAGAAAATCTCAAAATCTCTGCAATCAAGCAATTTACATAACCCTGGCTCTGGAGATACAGTTGGTCTTAATAAGACCTCAGGACCAAAGCAAGGGAGACCTTATGCAGCTGCAGCCGGTGCCAATTCTTCAGAAGTTCAGGCTTTGTCTAAGGAGATTACAGATCTCAAGCTCTCTGTGGACCTTTTGGAAAAAGAAAGGGACTTTTACTTTGCAAAATTAAGGGATATTGAAATTCTTTGTCAGACATCAGAGTTGGAGGATATCCCGATGTCGGTGGCGGTAAAGAAGATATTGTACGCAGCTGATGCGAAAGAATCACCACTTGCTGAAGCCCAGGAGTACCTCTACCAAACGCTAAACGTCGGTGAAGCCGAAGATGAAACCGAAGCAGAAGCTGAATCCGAAACAGAAACCGGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0000922 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009574 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009629 GO:0009630 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009958 GO:0010005 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030496 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0055028 GO:0060255 GO:0065007 GO:0065009 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

286

Amino Acids

31.88

Weight (kDa)

5.33

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 16 - 114 9.3e-10 Calponin homology (CH) domain
EB1 PF03271 207 - 245 2.7e-16 EB1-like C-terminal motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47690
fragaria_vesca FvH4_5g15190 FvH4_5g15190
malus_domestica MD00G1186200.v1.1 MD06G1191400.v1.1 MD14G1197800.v1.1
prunus_persica Prupe.5G189900_v2.0.a1 Prupe.5G189900_v2.0.a1
pyrus_communis pycom14g16430
rosa_chinensis RchiOBHm_Chr7g0180971
rosa_laevigata RLG00000005220
rosa_multiflora Rmu_sc0004056.1_g000004
rosa_roxburghii Rroxscaffold_3G00272370
rosa_rugosa Rorug06G0438000 Rorug06G0438100
rosa_samantha Rh7BG038800 Rh7DG039000
rosa_wichuraiana Rw7G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 551
AciI CCGC 1 cut(s) 713
AclI AACGTT 1 cut(s) 334
AclWI GGATC 3 cut(s) 74, 205, 420
AcsI RAATTY 2 cut(s) 314, 666
AcuI CTGAAG 3 cut(s) 65, 546, 783
AfaI GTAC 3 cut(s) 126, 731, 776
AfiI CCNNNNNNNGG 4 cut(s) 15, 482, 524, 706
AgeI ACCGGT 1 cut(s) 848
AgsI TTSAA 4 cut(s) 104, 244, 272, 665
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 3 cut(s) 154, 475, 768
AluBI AGCT 5 cut(s) 85, 542, 604, 737, 830
AluI AGCT 5 cut(s) 85, 542, 604, 737, 830
Alw21I GWGCWC 1 cut(s) 433
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 3 cut(s) 74, 205, 420
AlwNI CAGNNNCTG 1 cut(s) 830
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 5 cut(s) 110, 539, 542, 545, 734
ApoI RAATTY 2 cut(s) 314, 666
ArsI GACNNNNNNTTYG 2 cut(s) 626, 658
AsiGI ACCGGT 1 cut(s) 848
AspS9I GGNCC 2 cut(s) 515, 613
AsuHPI GGTGA 2 cut(s) 744, 812
AvaII GGWCC 2 cut(s) 515, 613
AxyI CCTNAGG 1 cut(s) 511
BanI GGYRCC 1 cut(s) 551
BarI GAAGNNNNNNTAC 2 cut(s) 713, 745
Bbv12I GWGCWC 1 cut(s) 433
BbvI GCAGC 5 cut(s) 97, 529, 551, 557, 746
BccI CCATC 1 cut(s) 133
BciT130I CCWGG 3 cut(s) 156, 477, 770
BcoDI GTCTC 1 cut(s) 523
BfmI CTRYAG 2 cut(s) 230, 543
BglII AGATCT 1 cut(s) 595
BisI GCNGC 5 cut(s) 111, 540, 543, 546, 735
BlsI GCNGC 5 cut(s) 112, 541, 544, 547, 736
Bme1390I CCNGG 3 cut(s) 156, 477, 770
Bme18I GGWCC 2 cut(s) 515, 613
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 515, 613
BmiI GGNNCC 1 cut(s) 553
BmrFI CCNGG 3 cut(s) 156, 477, 770
BmsI GCATC 4 cut(s) 122, 178, 274, 730
BpmI CTGGAG 1 cut(s) 504
BpuEI CTTGAG 1 cut(s) 584
BsaBI GATNNNNATC 1 cut(s) 417
BsaI GGTCTC 1 cut(s) 523
BsaJI CCNNGG 2 cut(s) 475, 768
BsaWI WCCGGW 1 cut(s) 848
BsaXI ACNNNNNCTCC 4 cut(s) 388, 418, 477, 507
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 482, 524, 706
Bse118I RCCGGY 2 cut(s) 548, 848
Bse21I CCTNAGG 1 cut(s) 511
Bse3DI GCAATG 1 cut(s) 329
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 3 cut(s) 156, 477, 770
BseDI CCNNGG 2 cut(s) 475, 768
BseGI GGATG 1 cut(s) 418
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 4 cut(s) 15, 482, 524, 706
BseMI GCAATG 1 cut(s) 329
BseMII CTCAG 2 cut(s) 446, 525
BseXI GCAGC 5 cut(s) 97, 529, 551, 557, 746
BshFI GGCC 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 551
BshTI ACCGGT 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 433
BsiSI CCGG 2 cut(s) 549, 849
BslFI GGGAC 1 cut(s) 647
BslI CCNNNNNNNGG 4 cut(s) 15, 482, 524, 706
BsmAI GTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 647
BsnI GGCC 1 cut(s) 298
Bso31I GGTCTC 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 433
Bsp1407I TGTACA 1 cut(s) 124
Bsp143I GATC 4 cut(s) 66, 210, 412, 595
BspACI CCGC 1 cut(s) 713
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 2 cut(s) 445, 524
BspLI GGNNCC 1 cut(s) 553
BspMAI CTGCAG 1 cut(s) 547
BspPI GGATC 3 cut(s) 74, 205, 420
BspT107I GGYRCC 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 329
BsrFI RCCGGY 2 cut(s) 548, 848
BsrGI TGTACA 1 cut(s) 124
BssAI RCCGGY 2 cut(s) 548, 848
BssECI CCNNGG 2 cut(s) 475, 768
BssMI GATC 4 cut(s) 66, 210, 412, 595
Bst2UI CCWGG 3 cut(s) 156, 477, 770
Bst4CI ACNGT 3 cut(s) 29, 341, 493
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 3 cut(s) 119, 551, 740
BstAUI TGTACA 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 296
BstDEI CTNAG 3 cut(s) 432, 511, 582
BstF5I GGATG 1 cut(s) 418
BstKTI GATC 4 cut(s) 69, 213, 415, 598
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 4 cut(s) 66, 210, 412, 595
BstMWI GCNNNNNNNGC 4 cut(s) 119, 545, 551, 740
BstNI CCWGG 3 cut(s) 156, 477, 770
BstSCI CCNGG 3 cut(s) 154, 475, 768
BstSFI CTRYAG 2 cut(s) 230, 543
BstV1I GCAGC 5 cut(s) 97, 529, 551, 557, 746
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
Bsu36I CCTNAGG 1 cut(s) 511
BsuRI GGCC 1 cut(s) 298
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 1 cut(s) 418
BtsIMutI CAGTG 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 296
CaiI CAGNNNCTG 1 cut(s) 830
Cfr10I RCCGGY 2 cut(s) 548, 848
Cfr13I GGNCC 2 cut(s) 515, 613
Csp6I GTAC 3 cut(s) 125, 730, 775
CspAI ACCGGT 1 cut(s) 848
CviQI GTAC 3 cut(s) 125, 730, 775
DdeI CTNAG 3 cut(s) 432, 511, 582
DpnI GATC 4 cut(s) 68, 212, 414, 597
DpnII GATC 4 cut(s) 66, 210, 412, 595
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 298
Eco31I GGTCTC 1 cut(s) 523
Eco32I GATATC 1 cut(s) 697
Eco47I GGWCC 2 cut(s) 515, 613
Eco57I CTGAAG 3 cut(s) 65, 546, 783
Eco81I CCTNAGG 1 cut(s) 511
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 3 cut(s) 154, 475, 768
EcoRV GATATC 1 cut(s) 697
FaiI YATR 9 cut(s) 146, 204, 209, 222, 350, 365, 378, 471, 537
FaqI GGGAC 1 cut(s) 647
Fnu4HI GCNGC 5 cut(s) 111, 540, 543, 546, 735
FokI GGATG 1 cut(s) 405
Fsp4HI GCNGC 5 cut(s) 111, 540, 543, 546, 735
GluI GCNGC 5 cut(s) 111, 540, 543, 546, 735
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 1 cut(s) 298
HapII CCGG 2 cut(s) 549, 849
HinfI GANTC 4 cut(s) 88, 344, 749, 833
HpaII CCGG 2 cut(s) 549, 849
HphI GGTGA 2 cut(s) 744, 812
Hpy166II GTNNAC 1 cut(s) 613
Hpy188I TCNGA 5 cut(s) 435, 565, 678, 685, 838
Hpy188III TCNNGA 5 cut(s) 214, 269, 483, 513, 700
Hpy8I GTNNAC 1 cut(s) 613
Hpy99I CGWCG 1 cut(s) 800
HpyAV CCTTC 3 cut(s) 89, 387, 403
HpyCH4III ACNGT 3 cut(s) 29, 341, 493
HpyCH4IV ACGT 3 cut(s) 62, 334, 795
HpyCH4V TGCA 7 cut(s) 113, 172, 322, 454, 539, 545, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 545, 551, 740
HpyF3I CTNAG 3 cut(s) 432, 511, 582
HpySE526I ACGT 3 cut(s) 62, 334, 795
Kzo9I GATC 4 cut(s) 66, 210, 412, 595
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 5 cut(s) 97, 529, 551, 557, 746
LweI GCATC 4 cut(s) 122, 178, 274, 730
MaeII ACGT 3 cut(s) 62, 334, 795
MaeIII GTNAC 1 cut(s) 335
MalI GATC 4 cut(s) 68, 212, 414, 597
MboI GATC 4 cut(s) 66, 210, 412, 595
MboII GAAGA 4 cut(s) 116, 552, 733, 821
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 433
MluCI AATT 8 cut(s) 181, 248, 255, 314, 463, 556, 650, 666
MmeI TCCRAC 2 cut(s) 33, 669
MnlI CCTC 4 cut(s) 100, 520, 685, 788
MseI TTAA 4 cut(s) 251, 288, 501, 653
MspA1I CMGCKG 3 cut(s) 85, 542, 737
MspI CCGG 2 cut(s) 549, 849
MspR9I CCNGG 3 cut(s) 156, 477, 770
MvaI CCWGG 3 cut(s) 156, 477, 770
MwoI GCNNNNNNNGC 4 cut(s) 119, 545, 551, 740
NdeII GATC 4 cut(s) 66, 210, 412, 595
NlaIV GGNNCC 1 cut(s) 553
PceI AGGCCT 1 cut(s) 298
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PfeI GAWTC 4 cut(s) 88, 344, 749, 833
PinAI ACCGGT 1 cut(s) 848
PkrI GCNGC 5 cut(s) 112, 541, 544, 547, 736
Psp1406I AACGTT 1 cut(s) 334
Psp6I CCWGG 3 cut(s) 154, 475, 768
PspGI CCWGG 3 cut(s) 154, 475, 768
PspN4I GGNNCC 1 cut(s) 553
PspPI GGNCC 2 cut(s) 515, 613
PstI CTGCAG 1 cut(s) 547
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 595
PvuII CAGCTG 3 cut(s) 85, 542, 737
RsaI GTAC 3 cut(s) 126, 731, 776
RsaNI GTAC 3 cut(s) 125, 730, 775
SaqAI TTAA 4 cut(s) 251, 288, 501, 653
SatI GCNGC 5 cut(s) 111, 540, 543, 546, 735
Sau3AI GATC 4 cut(s) 66, 210, 412, 595
Sau96I GGNCC 2 cut(s) 515, 613
ScrFI CCNGG 3 cut(s) 156, 477, 770
SduI GDGCHC 1 cut(s) 433
SfaNI GCATC 4 cut(s) 122, 178, 274, 730
SfcI CTRYAG 2 cut(s) 230, 543
SinI GGWCC 2 cut(s) 515, 613
SmlI CTYRAG 1 cut(s) 599
SmoI CTYRAG 1 cut(s) 599
Sse9I AATT 8 cut(s) 181, 248, 255, 314, 463, 556, 650, 666
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 1 cut(s) 713
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 3 cut(s) 154, 475, 768
TaaI ACNGT 3 cut(s) 29, 341, 493
TaiI ACGT 3 cut(s) 65, 337, 798
TaqI TCGA 1 cut(s) 415
TasI AATT 8 cut(s) 181, 248, 255, 314, 463, 556, 650, 666
TatI WGTACW 1 cut(s) 124
TfiI GAWTC 4 cut(s) 88, 344, 749, 833
Tru1I TTAA 4 cut(s) 251, 288, 501, 653
Tru9I TTAA 4 cut(s) 251, 288, 501, 653
TscAI CASTG 1 cut(s) 34
TseI GCWGC 5 cut(s) 110, 539, 542, 545, 734
TspDTI ATGAA 3 cut(s) 380, 384, 828
TspRI CASTG 1 cut(s) 34
VpaK11BI GGWCC 2 cut(s) 515, 613
XapI RAATTY 2 cut(s) 314, 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.